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array:23 [ "pii" => "S0301054611001121" "issn" => "03010546" "doi" => "10.1016/j.aller.2011.01.002" "estado" => "S300" "fechaPublicacion" => "2012-01-01" "aid" => "267" "copyright" => "SEICAP" "copyrightAnyo" => "2010" "documento" => "article" "crossmark" => 0 "subdocumento" => "fla" "cita" => "Allergol Immunopathol (Madr). 2012;40:14-9" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:2 [ "total" => 1799 "formatos" => array:3 [ "EPUB" => 10 "HTML" => 1452 "PDF" => 337 ] ] "itemSiguiente" => array:18 [ "pii" => "S030105461100019X" "issn" => "03010546" "doi" => "10.1016/j.aller.2010.10.006" "estado" => "S300" "fechaPublicacion" => "2012-01-01" "aid" => "250" "copyright" => "SEICAP" "documento" => "article" "crossmark" => 0 "subdocumento" => "fla" "cita" => "Allergol Immunopathol (Madr). 2012;40:20-4" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:2 [ "total" => 2044 "formatos" => array:3 [ "EPUB" => 9 "HTML" => 1506 "PDF" => 529 ] ] "en" => array:11 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>" "titulo" => "Effects of physical exercise on lung injury and oxidant stress in children with asthma" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "20" "paginaFinal" => "24" ] ] "contieneResumen" => array:1 [ "en" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Ozlem Gunay, Ece Onur, Ozge Yilmaz, Pinar E. Dundar, Canan Tikiz, Ahmet Var, Hasan Yuksel" "autores" => array:7 [ 0 => array:2 [ "nombre" => "Ozlem" "apellidos" => "Gunay" ] 1 => array:2 [ "nombre" => "Ece" "apellidos" => "Onur" ] 2 => array:2 [ "nombre" => "Ozge" "apellidos" => "Yilmaz" ] 3 => array:2 [ "nombre" => "Pinar E." "apellidos" => "Dundar" ] 4 => array:2 [ "nombre" => "Canan" "apellidos" => "Tikiz" ] 5 => array:2 [ "nombre" => "Ahmet" "apellidos" => "Var" ] 6 => array:2 [ "nombre" => "Hasan" "apellidos" => "Yuksel" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S030105461100019X?idApp=UINPBA00004N" "url" => "/03010546/0000004000000001/v1_201304101101/S030105461100019X/v1_201304101101/en/main.assets" ] "itemAnterior" => array:18 [ "pii" => "S0301054611000218" "issn" => "03010546" "doi" => "10.1016/j.aller.2010.11.002" "estado" => "S300" "fechaPublicacion" => "2012-01-01" "aid" => "252" "copyright" => "SEICAP" "documento" => "article" "crossmark" => 0 "subdocumento" => "fla" "cita" => "Allergol Immunopathol (Madr). 2012;40:9-13" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:2 [ "total" => 1637 "formatos" => array:3 [ "EPUB" => 11 "HTML" => 1152 "PDF" => 474 ] ] "en" => array:11 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>" "titulo" => "The analysis of Hymenoptera hypersensitive patients in Ankara, Turkey" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "9" "paginaFinal" => "13" ] ] "contieneResumen" => array:1 [ "en" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Gul Karakaya, Ebru Celebioglu, A. Ugur Demir, A. Fuat Kalyoncu" "autores" => array:4 [ 0 => array:2 [ "nombre" => "Gul" "apellidos" => "Karakaya" ] 1 => array:2 [ "nombre" => "Ebru" "apellidos" => "Celebioglu" ] 2 => array:2 [ "nombre" => "A. Ugur" "apellidos" => "Demir" ] 3 => array:2 [ "nombre" => "A. Fuat" "apellidos" => "Kalyoncu" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0301054611000218?idApp=UINPBA00004N" "url" => "/03010546/0000004000000001/v1_201304101101/S0301054611000218/v1_201304101101/en/main.assets" ] "en" => array:17 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original Article</span>" "titulo" => "Aging and asthma – changes in CD45RA, CD29 and CD95 T cells subsets" "tieneTextoCompleto" => true "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "14" "paginaFinal" => "19" ] ] "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "A. Todo-Bom, A. Mota-Pinto, V. Alves, M. Santos-Rosa" "autores" => array:4 [ 0 => array:4 [ "nombre" => "A." "apellidos" => "Todo-Bom" "email" => array:1 [ 0 => "flcosta@netcabo.pt" ] "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">¿</span>" "identificador" => "cor0005" ] ] ] 1 => array:3 [ "nombre" => "A." "apellidos" => "Mota-Pinto" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] ] ] 2 => array:3 [ "nombre" => "V." "apellidos" => "Alves" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] ] ] 3 => array:3 [ "nombre" => "M." "apellidos" => "Santos-Rosa" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">d</span>" "identificador" => "aff0020" ] ] ] ] "afiliaciones" => array:4 [ 0 => array:3 [ "entidad" => "ImmunoAllergology Department, Coimbra University Hospital, General Pathology Institute, Faculty of Medicine - University of Coimbra, 3004-504 – Coimbra, Portugal" "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "General Pathology Institute, Faculty of Medicine - University of Coimbra, Coimbra, Portugal" "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] 2 => array:3 [ "entidad" => "Investigator, Immunology Institute, Faculty of Medicine - University of Coimbra, Coimbra, Portugal" "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] 3 => array:3 [ "entidad" => "Immunology Institute, Faculty of Medicine - University of Coimbra, Coimbra, Portugal" "etiqueta" => "<span class="elsevierStyleSup">d</span>" "identificador" => "aff0020" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Thymopoiesis provides the immune system with T cells with a naive phenotype (CD45RA, CD62L CD27, CD28) and although this procedure suffers a reduction during the growth process it can persist during adulthood.<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1,2</span></a> Antigen exposure determines the expression of new surface molecules and confers proliferative properties to cells transforming them into effector and memory cells. Repeated stimulation with identical antigenic proteins elicits responses of cross-reactivity increasing the survival of memory T lymphocytes (TL) and the expansion of these cell lines, while limiting the expansion of naive cell lines.<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">The most profound age related changes described within T cell populations are the decrease in the number of naive cells and the accumulation of sensitised cells. Moreover, a further decrease of this lymphocyte population can be observed when the exposure to new pathogens induces the replacement of old memories for new ones.<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> The decrease of naive T lymphocytes contributes to the impoverishment of lymphocyte repertoires with defective responses to new antigens.<a class="elsevierStyleCrossRefs" href="#bib0020"><span class="elsevierStyleSup">4,5</span></a> However, a substantial naive T-cell pool is maintained even in centenarian individuals and some data suggest that their relative and absolute values may remain virtually unchanged after 40<span class="elsevierStyleHsp" style=""></span>years of life. Extrathymic differentiation of TL can become prevalent in the elderly.<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">6</span></a> Immunohistological studies showed a reduction in the number of CD45RA naive lymphocytes in lymph nodes associated to aging.<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">The CD29 is the β1 subunit of the integrin family from adhesion receptors that is expressed when a cell enters the G1 phase of the cell cycle. This molecule is often present in memory cells and tends to increase in the elderly.<a class="elsevierStyleCrossRefs" href="#bib0040"><span class="elsevierStyleSup">8,9</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">Apoptosis is a key mechanism in the elimination of activated cells that expand in excess during the course of a physiological immune response. The Fas death receptor (also named CD95 or APO-1) is an integral part of the physiological regulation of apoptosis. In aging, the process of apoptosis establishes the preferential elimination of memory senescent cells and its deregulation is associated to disease.<a class="elsevierStyleCrossRefs" href="#bib0050"><span class="elsevierStyleSup">10,11</span></a></p><p id="par0025" class="elsevierStylePara elsevierViewall">Several environmental factors and health conditions have a recognised capacity for eliciting cell activation into memory and effectors populations and to trigger apoptosis with varying impact on successful aging.<a class="elsevierStyleCrossRef" href="#bib0060"><span class="elsevierStyleSup">12</span></a></p><p id="par0030" class="elsevierStylePara elsevierViewall">In allergic asthma there is a continuous stimulation of TL by several aeroallergens, which can lead to a decrease in naive T cells. A decrease in apoptosis can also help to sustain the airway inflammation that characterises the disease.</p><p id="par0035" class="elsevierStylePara elsevierViewall">The aim of this work was to evaluate the imbalance between CD45 and CD29 cells during the aging process and to analyse the changes in these cell phenotypes in elderly asthma patients. A second goal was to compare susceptibility to apoptosis and how it affects aging and chronic respiratory diseases such as asthma.</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Population</span><p id="par0040" class="elsevierStylePara elsevierViewall">A group of 58 elderly healthy volunteers older than 65<span class="elsevierStyleHsp" style=""></span>years of age (mean age 79.2<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>7.1); a group of 33 healthy young adults (mean age 30.2+11.3<span class="elsevierStyleHsp" style=""></span>years); and a group of 95 individuals with controlled asthma also older than 65<span class="elsevierStyleHsp" style=""></span>years of age (mean age 72.4<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>5.1<span class="elsevierStyleHsp" style=""></span>years) attending a chest disease outpatient department were studied. All the patients had a history of intermittent chest tightness, wheezing or shortness of breath for at least 30<span class="elsevierStyleHsp" style=""></span>years prior to study participation consistent with the diagnosis of asthma according to the Global Initiative for Asthma.<a class="elsevierStyleCrossRef" href="#bib0065"><span class="elsevierStyleSup">13</span></a> All the patients were using 250<span class="elsevierStyleHsp" style=""></span>μg to 500<span class="elsevierStyleHsp" style=""></span>μg of beclometasone dipropionate in mild to moderate asthma or 250<span class="elsevierStyleHsp" style=""></span>μg of fluticasone propionate daily associated with long-acting ß2-agonists in severe asthma and short-acting ß2-agonists as needed. Other anti-asthmatic drugs were withdrawn at least four weeks prior to the study. All patients were examined by a physician and underwent spirometric testing using the same equipment (Vitalograph Compact) at least six hours after the last dose of any bronchodilator. Predicted values were measured according to Knudson et al.<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">14</span></a> Subjects’ spirometric performances were assessed by means of a computerised program following the American Thoracic Society (ATS) 94 criteria. Approval for analysis was determined using the ATS 94 criteria, and accuracy was achieved if, within the same evaluation, three curves were acceptable and reproducible. Patients with severe asthma had FEV1 percentage values in spirometry lower than 60% of the predicted (<a class="elsevierStyleCrossRefs" href="#tbl0005">Tables 1, 2</a>).</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><p id="par0045" class="elsevierStylePara elsevierViewall">All the studied individuals were able to perform their own daily tasks, carrying out regular physical activity. None of them had had any respiratory infections in the previous month to their inclusion in the study. No other clinically relevant diseases were reported.</p><p id="par0050" class="elsevierStylePara elsevierViewall">The following were considered exclusion criteria: smoking, cancer, autoimmune disease, infection, diabetes, heart failure, renal failure, chronic hepatic disease, and recent exposure to environmental risk factors for pulmonary diseases.</p><p id="par0055" class="elsevierStylePara elsevierViewall">Approval to this study was received from institutional review board of the Coimbra University Hospital, and all individuals were selected after informed consent.</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Methods</span><p id="par0060" class="elsevierStylePara elsevierViewall">Skin prick tests to 20 common aeroallergens were performed to all individuals involved in the study (ALKABELLO/Lancetter-tames Hollister Stier). Subjects were classified as allergic with one positive test associated with clinical symptoms. The remaining asthmatics were classified as non-allergic.</p><p id="par0065" class="elsevierStylePara elsevierViewall">Immunoglobulin G (IgG), IgA and IgM were measured in total serum by nephelometry and total serum IgE was measured using a commercial kit (Coat-A-Count® Total IgE IRMA, DPC®, CA, USA) based on an Immuno-radiometric assay (IRMA) of solid phase. This kit uses monoclonal antibodies anti-IgE with I125-labelled in the liquid phase and polyclonal antibodies anti-IgE attached to the wall of the polystyrene tube.</p><p id="par0070" class="elsevierStylePara elsevierViewall">Therefore, the IgE present in the sample was retained between these two antibodies. These tubes were measured using a gamma counter (Gamma-C-12, DPC®, CA, USA) for one minute. Total IgE concentration in the sample was directly proportional to the counts per minute. This concentration was determined by comparison with provided calibrators. The kit sensitivity was 0.5 IU/ml.</p><p id="par0075" class="elsevierStylePara elsevierViewall">The study of peripheral blood (PB) lymphocytes was performed using flow cytometry assay. 100ul of PB was incubated with monoclonal antibodies for 10<span class="elsevierStyleHsp" style=""></span>minutes at room temperature in the dark. To determine the main populations, a single tube with Lymphogram (Cytognos, Salamanca, Spain) containing monoclonal antibodies anti-CD4 stained with phycoerythrin cyanin 5 (PECy5), CD8 with fluorescein-isothiocyanate (FITC), CD3 with phycoerythrin (PE) was used. Moreover we evaluated CD45RA, CD29 and CD95 (Immunotech, Marseille, France) stained with FITC, CD8 (Dako, Denmark) with PE, CD3 and CD4 with PECy5 (Dako, Denmark), according to manufacturers’ specifications.</p><p id="par0080" class="elsevierStylePara elsevierViewall">Flow Cytometry (FACS) data were collected on a FACS Calibur (BD Biosciences, San Jose, CA, USA) using the Cellquest® acquisition software and analysed by Paint-a-gate® software.</p></span></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Statistical analysis</span><p id="par0085" class="elsevierStylePara elsevierViewall">Statistical analysis was performed using SPSS 12.0 software package. The Kolmogorov –Smirnov test was used to check if variables were normally distributed. For those with a normal distribution the parametric t test was used for two independent samples. Variables that were not distributed normally were evaluated using the Mann-Whitney non-parametric test. P-values less than 0.05 were considered significant. Statistical comparisons were made between elderly healthy volunteers and healthy young adults and between elderly asthmatics and healthy elderly individuals. Differences between allergic and non-allergic asthmatic patients were also analysed.</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Results</span><p id="par0090" class="elsevierStylePara elsevierViewall">Sixty-five asthmatic patients had positive tests to at least one allergen. The control population and the remaining asthmatics had negative skin prick tests.</p><p id="par0095" class="elsevierStylePara elsevierViewall">Serum immunoglobulin (Ig) IgG, IgA and IgE were higher and IgM lower in allergic patients when compared to non-allergic patients (<a class="elsevierStyleCrossRef" href="#tbl0015">Table 3</a>).</p><elsevierMultimedia ident="tbl0015"></elsevierMultimedia><p id="par0100" class="elsevierStylePara elsevierViewall">The percentual values of CD3,CD4 and CD8 blood lymphocytes were within normal ranges. CD3 lymphocyte percentage in asthmatic patients was similar to the control groups and CD3+CD8+ lymphocytes were slightly decreased without statistical significance in asthmatic patients compared to control group of the same age. Only CD3+CD4+ cells were significantly increased in asthmatic patients (<a class="elsevierStyleCrossRef" href="#tbl0020">Table 4</a>).</p><elsevierMultimedia ident="tbl0020"></elsevierMultimedia><p id="par0105" class="elsevierStylePara elsevierViewall">The mean percentage of naive CD45RA and CD4+ CD45RA + decreased significantly in the elderly individuals studied. The mean percentage of naive CD45RA was also significantly deceased in asthmatic patients when compared to the control group of the same age. CD4+ CD45RA + and CD8+CD45RA + cells were slightly reduced in asthmatics (<a class="elsevierStyleCrossRef" href="#tbl0025">Table 5</a>).</p><elsevierMultimedia ident="tbl0025"></elsevierMultimedia><p id="par0110" class="elsevierStylePara elsevierViewall">The mean percentage of CD4+CD29+ and CD8+CD29+ lymphocytes in the elderly was significantly increased both in asthmatics and healthy individuals. The CD8+CD29+ percentage in the asthmatic patient group was above the percentage found in the young control group but was significantly decreased when compared to the elderly control group (<a class="elsevierStyleCrossRef" href="#tbl0025">Table 5</a>).</p><p id="par0115" class="elsevierStylePara elsevierViewall">The mean percentage of CD95 lymphocytes in elderly health individuals was significantly increased when compared to the young control group. Conversely, the CD95 lymphocytes in the asthmatic patients group was significantly decreased when compared to the elderly control group although the values found were still higher than those of the younger healthy individuals (<a class="elsevierStyleCrossRef" href="#tbl0025">Table 5</a>).</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Discussion and conclusions</span><p id="par0120" class="elsevierStylePara elsevierViewall">Although the skin reactivity and IgE values tend to decrease with age, elderly asthmatics have higher sensitisation to environmental allergens than the general population of the same age group. The asthmatics studied had intense cutaneous reactivity to aeroallergens, a Th2 immune response conserved and high levels of total serum IgE when compared to the control group (p<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>. 0001)<a class="elsevierStyleCrossRefs" href="#bib0075"><span class="elsevierStyleSup">15–17</span></a> The asthmatics also presented a significant increase of CD3+CD4+ lymphocytes compared to the control group (p<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05), which can be associated to preserved humoral response despite the long disease evolution of the patients studied.</p><p id="par0125" class="elsevierStylePara elsevierViewall">A crucial problem of the immune system in the elderly is the reduced proliferative cell capacity and the delay of clonal expansion in response to antigenic exposure as a result of stem cell decrease. The production of specific antibodies in response to vaccines and to environmental antigens is reduced in elderly but the production of autoantibodies is increased and the total serum immunoglobulin levels remain unchanged.<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">18</span></a> A selective decline in IL2 and IFN γ is observed while IL4 and IL6 production is kept within normal values. This adjustment in the cytokines balance is accomplished by a normal or increased humoral response and a decrease in cellular response.<a class="elsevierStyleCrossRef" href="#bib0095"><span class="elsevierStyleSup">19</span></a> The CD45 family (common leukocyte antigen) consists of multiple isoforms, which differ only in length and glycosylation of their extracellular domains. The different distribution of these isoforms in leukocytes and in particular on T cells plays a crucial role in cell activation and pathways signalling mediated by this receptor. The expression of different isoforms of CD45R is thus regulated during the maturation and activation of T cells. T cells with a naive phenotype express CD45RA differentiation markers and diminish throughout life, which is associated with a gradual onset of memory phenotypes. When asthmatics are exposed to allergen stimulation only CD45R0 cells can express activation markers.<a class="elsevierStyleCrossRefs" href="#bib0100"><span class="elsevierStyleSup">20,21</span></a></p><p id="par0130" class="elsevierStylePara elsevierViewall">This study showed a significant reduction in the expression of CD45RA in elderly health individuals when compared with a young control group (p<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05). This finding confirms the reported decrease of naive cells during the aging process. It was also decided to evaluate if the asthma condition posed an additional risk for this reduction. In fact the reported reduction was even more evident in elderly asthmatics (p<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05). The expression of CD45RA on CD4 and CD8 was also reduced in asthma. The CD4+CD45RA+ subtype reached 16% while the values of CD8+CD45RA+ exceeded 20%.</p><p id="par0135" class="elsevierStylePara elsevierViewall">The CD45RA can be expressed in cells previously activated that entered in a resting state. This decreased response to stimulation and lower functional activity, is probably linked to a lack of IL2 production.<a class="elsevierStyleCrossRefs" href="#bib0110"><span class="elsevierStyleSup">22,23</span></a> In asthmatics the existence of a continued exposure to allergenic sources or other airborne hazardous substances is accepted, resulting in a continuous stimulation of the immune system, mainly of the Th2 cells. This stimulation will sustain the setting up of memory phenotypes particularly in chronic diseases. Even in non-allergic asthmatics the bronchi inflammatory response is connected with exposure to inhalatory triggers. This phenomenon was also described in individuals with chronic infections where the offending agent was present as a persistent stimulus for the immune response. In this age group a marked reduction in the ability of identifying new allergens and promoting an effective immune response is observed. This reduction in acquisition of new skills is connected to the preservation of memory phenotypes acquired throughout life, which are specific to an extensive repertoire of antigens. It is therefore assured a prompt and effective response to the recall antigens, while there is a restrict response to new challenges.<a class="elsevierStyleCrossRefs" href="#bib0120"><span class="elsevierStyleSup">24,25</span></a> Naive cells are essential in the defence against acute infections and as so their decrease in elderly asthma, particularly in CD8 phenotypes, should be regarded as a bad prognosis factor.</p><p id="par0140" class="elsevierStylePara elsevierViewall">The CD29 receptor is a homing molecule expressed in activated cells that potentiates T cell adhesion to endothelium and is involved in cellular response to specific antigenic challenge. The CD29 is preferentially expressed in CD4+CD45R0+ memory cells. In asthma this molecule tends to increase following allergen stimulation, and is especially associated with memory phenotypes.<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">26</span></a> CD29 lymphocytes were significantly increased both in elderly healthy individuals and elderly asthmatics. The mechanism leading to increased expression in aging is probably dominant and therefore overlaps the arise expected from asthma.<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a></p><p id="par0145" class="elsevierStylePara elsevierViewall">If we analyse the elderly population, a significant negative correlation between CD29 and CD45RA is observed meaning that the presence of a higher number of cells marked by CD45RA is associated to a smaller number of positive cells for CD29, both in asthmatics (PC<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>-0.25, p<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05) and in group control (PC<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>-0.45, p<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05). This observation strengthens the fact that the decrease of naive cells tends to be inversely related to the expression of CD29, a marker of activation and homing present in memory cells also in elderly.</p><p id="par0150" class="elsevierStylePara elsevierViewall">Apoptosis is a natural defence mechanism against disabled or dead cells that are harmful to the physical integrity of the individual. Susceptibility to apoptosis measured by CD95 expression is reflected by an increase of this receptor in the elderly and suggests that apoptosis is important in the aging processes.<a class="elsevierStyleCrossRefs" href="#bib0130"><span class="elsevierStyleSup">26,27</span></a> The studied elderly healthy individuals had a significantly increased value of CD95 compared to the control group of young adults (p<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.0001) and compared to asthmatic patients (p<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.0001). A positive correlation between age and CD95 was found (PC<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.446, p<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05). The resistance of T lymphocytes to apoptosis, namely the reduction of expression of receptors that can initiate this process of cell death, could act by promoting the maintenance of activated and memory cells and their intervention in the inflammatory process characteristic of bronchial asthma.<a class="elsevierStyleCrossRefs" href="#bib0140"><span class="elsevierStyleSup">28–31</span></a> In asthma there is a higher probability of decreased expression of apoptosis markers by blood cells overcoming, in some cases, the growing trend associated with the aging process. Recent studies have shown an increased incidence of cancer, particularly lung cancer, in elderly asthmatics.<a class="elsevierStyleCrossRefs" href="#bib0160"><span class="elsevierStyleSup">32,33</span></a> The observed reduction of markers expected to initiate processes of apoptosis can also promote the persistence of abnormal or damaged cells and consequent neoplasic condition.</p><p id="par0155" class="elsevierStylePara elsevierViewall">The better knowledge of the immunoinflammatory pathway of elderly asthmatics can provide important information to improve the outcome of these patients.</p></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Conflict of interest</span><p id="par0170" class="elsevierStylePara elsevierViewall">The authors have no conflict of interest to declare.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:9 [ 0 => array:2 [ "identificador" => "xres86066" "titulo" => array:5 [ 0 => "Summary" 1 => "Background" 2 => "Methods" 3 => "Results" 4 => "Conclusions" ] ] 1 => array:2 [ "identificador" => "xpalclavsec74229" "titulo" => "Keywords" ] 2 => array:2 [ "identificador" => "sec0005" "titulo" => "Introduction" ] 3 => array:3 [ "identificador" => "sec0010" "titulo" => "Methods" "secciones" => array:2 [ 0 => array:2 [ "identificador" => "sec0015" "titulo" => "Population" ] 1 => array:2 [ "identificador" => "sec0020" "titulo" => "Methods" ] ] ] 4 => array:2 [ "identificador" => "sec0025" "titulo" => "Statistical analysis" ] 5 => array:2 [ "identificador" => "sec0030" "titulo" => "Results" ] 6 => array:2 [ "identificador" => "sec0035" "titulo" => "Discussion and conclusions" ] 7 => array:2 [ "identificador" => "sec0040" "titulo" => "Conflict of interest" ] 8 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2010-11-23" "fechaAceptado" => "2011-01-18" "PalabrasClave" => array:1 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec74229" "palabras" => array:7 [ 0 => "Asthma" 1 => "Elderly" 2 => "Allergy" 3 => "T cell" 4 => "CD45RA" 5 => "CD29" 6 => "CD95" ] ] ] ] "tieneResumen" => true "resumen" => array:1 [ "en" => array:2 [ "titulo" => "Summary" "resumen" => "<span class="elsevierStyleSectionTitle">Background</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Aging is associated with thymus involution leading to a reduction in naive T cells and to an accumulation of effector-memory cells. Apoptosis is a key mechanism to clear the immune system from activated and harmful cells. In asthma the stimulation of T cells by environmental antigens can decrease naive cells and sustain activated cells. The aim of this work was to evaluate the imbalance between CD45RA and CD29 cells during the aging process and their changes in elderly asthma and to evaluate how elderly and chronic diseases like asthma can affect susceptibility to apoptosis.</p> <span class="elsevierStyleSectionTitle">Methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Elderly and young adult healthy volunteers and elderly asthmatic patients were submitted to skin prick tests, immunoglobulin determination and flow cytometry analyses of CD3, CD4, CD8, CD45RA, CD29, and CD95.</p> <span class="elsevierStyleSectionTitle">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Serum IgE was increased in allergic patients (p<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.0001). Asthmatics presented an increase in CD4 cells (p<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05). CD45RA was significantly decreased in elderly individuals (p<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05) and this decrease was higher in asthmatics (p<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05). CD29 was increased in elderly healthy individuals compared to the control young group (p<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.0001). A negative correlation between CD29 and CD45RA (p<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05) was observed. CD95 lymphocytes increased in elderly (p<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.0001) and a positive correlation between age and CD95 (p<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05) was found. Asthmatic patients showed significant decreases in CD95 (p<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0. 0001).</p> <span class="elsevierStyleSectionTitle">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Naive cells are key cells in the defence against infections and their decrease in the elderly and in asthma is a bad prognosis factor. The reduction of apoptosis markers can promote the persistence of activated cells involved in chronic conditions.</p>" ] ] "multimedia" => array:5 [ 0 => array:7 [ "identificador" => "tbl0005" "etiqueta" => "Table 1" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "tabla" => array:1 [ "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Population \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">n \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Gender (M/F) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Mean age \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Range \t\t\t\t\t\t\n \t\t\t\t</td></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">Control Elderly</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">58 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">19/39 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">79.2<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>7.0 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">65/94 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">Control Young</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">33 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">8/25 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">30.2+11.3 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">22/38 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">Asthmatics</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">95 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">32/63 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">72.4<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>5.1 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">65/87 \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab165363.png" ] ] ] ] "descripcion" => array:1 [ "en" => "<p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">Gender and age distribution in control and asthmatic groups.</p>" ] ] 1 => array:7 [ "identificador" => "tbl0010" "etiqueta" => "Table 2" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "tabla" => array:2 [ "leyenda" => "<p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Predicted values were measured according to Knudson et al.<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">14</span></a></p><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Fev1 - Forced expiratory volume in one second; Fev1BD - <span class="elsevierStyleItalic">FEV1</span> after bronchodilatation;</p><p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">VC - vital capacity.</p>" "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">n \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Fev1 (%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Fev1% BD \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">VC% \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Tiffeneau \t\t\t\t\t\t\n \t\t\t\t</td></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">Severe Asthma</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">19 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">51.8<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>10.0 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">10.2<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>3.7 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">68.1<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>10.2 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">70.1<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>9.7 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">Mild Asthma</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t">76 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">87.6<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>17.8 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">12.4<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>6.2 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">94.5<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>15.5 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">83.5<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>13.0 \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab165362.png" ] ] ] ] "descripcion" => array:1 [ "en" => "<p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Average percentage of predicted spirometric values in asthmatics.</p>" ] ] 2 => array:7 [ "identificador" => "tbl0015" "etiqueta" => "Table 3" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "tabla" => array:3 [ "leyenda" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">IgG, IgM and IgA are expressed in mg/l; IgE is expressed in IU</p>" "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">IgG \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">IgA \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">IgM \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">IgE \t\t\t\t\t\t\n \t\t\t\t</td></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">Allergic asthma</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">11.6<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>2.7 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">3.2<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>2.1 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">1.2<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>1.4 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">362<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>310/<a class="elsevierStyleCrossRef" href="#tblfn0005"><span class="elsevierStyleSup">a</span></a> \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">Non allergic asthma</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">10.8<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>2.3/ns \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">3.1<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>2.2/ns \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">1.3<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>0.7/ns \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">92<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>193 \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab165364.png" ] ] ] "notaPie" => array:1 [ 0 => array:3 [ "identificador" => "tblfn0005" "etiqueta" => "a" "nota" => "<p class="elsevierStyleNotepara">statistical significance p<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.0001; ns = no significance</p>" ] ] ] "descripcion" => array:1 [ "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">Serum Igs values in asthmatics.</p>" ] ] 3 => array:7 [ "identificador" => "tbl0020" "etiqueta" => "Table 4" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "tabla" => array:2 [ "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Control Young \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Control Elderly \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Asthma \t\t\t\t\t\t\n \t\t\t\t</td></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">CD3</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">75.0<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>3.1 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">73.6<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>9.9/ns \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">73.4<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>7.6/ns \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">CD3+CD4+</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">43.2<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>5.1 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">41.8<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>10.4/ns \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">45.4<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>9.6/<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">a</span></a> \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">CD3+CD8+</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">22.2<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>4.3 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">25.0<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>9.5/ns \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">22.9<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>8.0/<span class="elsevierStyleItalic">ns</span> \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab165365.png" ] ] ] "notaPie" => array:1 [ 0 => array:3 [ "identificador" => "tblfn0010" "etiqueta" => "a" "nota" => "<p class="elsevierStyleNotepara">statistical significance p<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05; ns = no significance</p>" ] ] ] "descripcion" => array:1 [ "en" => "<p id="spar0060" class="elsevierStyleSimplePara elsevierViewall">Percentual values of lymphocytes populations in asthmatics and control groups.</p>" ] ] 4 => array:7 [ "identificador" => "tbl0025" "etiqueta" => "Table 5" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "tabla" => array:2 [ "leyenda" => "<p id="spar0070" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleSup">a</span> Statistical significance p<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.0001. ns = no significance.</p><p id="spar0075" class="elsevierStyleSimplePara elsevierViewall">* Only done in 20 individuals (mean age 37.8<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>12.2 years).</p>" "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Control Young \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Elderly Control \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Allergic asthma \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t" style="border-bottom: 2px solid black">Non-allergic asthma \t\t\t\t\t\t\n \t\t\t\t</td></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">CD45RA</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">64.3<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>7.2 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">59.6<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>9.8/<span class="elsevierStyleSup">a</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">55.3<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>9.6/<span class="elsevierStyleSup">a</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">54.0<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>10.4/<span class="elsevierStyleSup">a</span> \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">CD45RA+CD4+</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">22.4<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>6.4 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">16.2<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>9.6/<span class="elsevierStyleSup">a</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">16.2<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>10.0/ns \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">14.8<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>5.0/ns \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">CD45RA+CD8+</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">21.5<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>4.1 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">22.8<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>8.0/ns \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">20.4<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>6.9/ns \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">20.0<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>7.0/ns \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">CD29+CD4+</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">20.1<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>2.1* \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">29.7<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>7.5/<span class="elsevierStyleSup">a</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">27.4<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>7.2/ns \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">28.7<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>7.0/ns \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">CD29+CD8+</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">14.2<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>3.2* \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">27.4<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>9.7/<span class="elsevierStyleSup">a</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">23.1<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>7.7/<span class="elsevierStyleSup">a</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">23.0<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>8.1/<span class="elsevierStyleSup">a</span> \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="\n \t\t\t\t\ttop\n \t\t\t\t"><span class="elsevierStyleBold">CD95</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">13.8<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>10.8* \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">52.6<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>12.1<span class="elsevierStyleSup">a</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">39.7<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>18.6<span class="elsevierStyleSup">a</span> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="\n \t\t\t\t\ttop\n \t\t\t\t">39.5<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>17.9<span class="elsevierStyleSup">a</span> \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab165361.png" ] ] ] ] "descripcion" => array:1 [ "en" => "<p id="spar0065" class="elsevierStyleSimplePara elsevierViewall">Percentual values of 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2024 November | 6 | 0 | 6 |
2024 October | 29 | 7 | 36 |
2024 September | 32 | 3 | 35 |
2024 August | 31 | 3 | 34 |
2024 July | 24 | 7 | 31 |
2024 June | 14 | 4 | 18 |
2024 May | 24 | 3 | 27 |
2024 April | 24 | 4 | 28 |
2024 March | 18 | 13 | 31 |
2024 February | 24 | 6 | 30 |
2024 January | 403 | 8 | 411 |
2023 December | 31 | 4 | 35 |
2023 November | 26 | 4 | 30 |
2023 October | 46 | 14 | 60 |
2023 September | 24 | 17 | 41 |
2023 August | 15 | 4 | 19 |
2023 July | 25 | 3 | 28 |
2023 June | 19 | 5 | 24 |
2023 May | 11 | 5 | 16 |
2023 April | 8 | 5 | 13 |
2023 March | 10 | 3 | 13 |
2023 February | 12 | 9 | 21 |
2023 January | 19 | 10 | 29 |
2022 December | 21 | 9 | 30 |
2022 November | 17 | 11 | 28 |
2022 October | 13 | 14 | 27 |
2022 September | 23 | 10 | 33 |
2022 August | 13 | 6 | 19 |
2022 July | 13 | 8 | 21 |
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2022 May | 15 | 14 | 29 |
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2021 July | 9 | 9 | 18 |
2021 June | 18 | 9 | 27 |
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2021 March | 28 | 8 | 36 |
2021 February | 14 | 6 | 20 |
2021 January | 12 | 10 | 22 |
2020 December | 1 | 2 | 3 |
2020 November | 0 | 4 | 4 |
2020 October | 0 | 1 | 1 |
2020 September | 0 | 1 | 1 |
2020 July | 0 | 1 | 1 |
2020 May | 0 | 2 | 2 |
2020 April | 0 | 2 | 2 |
2020 March | 0 | 3 | 3 |
2020 February | 0 | 5 | 5 |
2019 December | 0 | 1 | 1 |
2019 September | 0 | 2 | 2 |
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2019 July | 0 | 3 | 3 |
2019 June | 0 | 13 | 13 |
2019 May | 0 | 7 | 7 |
2019 April | 0 | 2 | 2 |
2019 March | 0 | 2 | 2 |
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2018 February | 5 | 4 | 9 |
2018 January | 6 | 0 | 6 |
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2017 October | 5 | 4 | 9 |
2017 September | 7 | 1 | 8 |
2017 August | 15 | 3 | 18 |
2017 July | 6 | 2 | 8 |
2017 June | 13 | 10 | 23 |
2017 May | 17 | 2 | 19 |
2017 April | 8 | 1 | 9 |
2017 March | 7 | 5 | 12 |
2017 February | 10 | 3 | 13 |
2017 January | 10 | 3 | 13 |
2016 December | 9 | 6 | 15 |
2016 November | 11 | 2 | 13 |
2016 October | 14 | 0 | 14 |
2016 September | 13 | 1 | 14 |
2016 August | 12 | 3 | 15 |
2016 July | 5 | 1 | 6 |
2016 June | 18 | 3 | 21 |
2016 May | 7 | 12 | 19 |
2016 April | 11 | 11 | 22 |
2016 March | 11 | 7 | 18 |
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2015 October | 19 | 2 | 21 |
2015 September | 8 | 1 | 9 |
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2015 May | 10 | 4 | 14 |
2015 April | 12 | 5 | 17 |
2015 March | 3 | 2 | 5 |
2015 February | 28 | 4 | 32 |
2015 January | 33 | 5 | 38 |
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2014 September | 31 | 4 | 35 |
2014 August | 18 | 3 | 21 |
2014 July | 12 | 1 | 13 |
2014 June | 14 | 5 | 19 |
2014 May | 3 | 2 | 5 |
2014 April | 10 | 1 | 11 |
2014 March | 39 | 11 | 50 |
2014 February | 29 | 3 | 32 |
2014 January | 23 | 7 | 30 |
2013 December | 25 | 5 | 30 |
2013 November | 23 | 9 | 32 |
2013 October | 41 | 11 | 52 |
2013 September | 23 | 15 | 38 |
2013 August | 34 | 5 | 39 |
2013 July | 35 | 7 | 42 |
2013 June | 16 | 0 | 16 |
2013 May | 27 | 8 | 35 |
2013 April | 18 | 8 | 26 |
2013 March | 24 | 4 | 28 |
2013 February | 23 | 10 | 33 |
2013 January | 16 | 3 | 19 |
2012 December | 10 | 5 | 15 |
2012 November | 0 | 3 | 3 |
2012 October | 2 | 3 | 5 |
2012 January | 475 | 0 | 475 |