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array:23 [ "pii" => "S0301054616300428" "issn" => "03010546" "doi" => "10.1016/j.aller.2016.02.007" "estado" => "S300" "fechaPublicacion" => "2016-09-01" "aid" => "753" "copyright" => "SEICAP" "copyrightAnyo" => "2016" "documento" => "article" "crossmark" => 1 "subdocumento" => "fla" "cita" => "Allergol Immunopathol (Madr). 2016;44:467-71" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:2 [ "total" => 85 "formatos" => array:3 [ "EPUB" => 10 "HTML" => 54 "PDF" => 21 ] ] "itemSiguiente" => array:18 [ "pii" => "S0301054616300143" "issn" => "03010546" "doi" => "10.1016/j.aller.2015.12.003" "estado" => "S300" "fechaPublicacion" => "2016-09-01" "aid" => "742" "copyright" => "SEICAP" "documento" => "article" "crossmark" => 1 "subdocumento" => "fla" "cita" => "Allergol Immunopathol (Madr). 2016;44:472-4" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:2 [ "total" => 69 "formatos" => array:3 [ "EPUB" => 9 "HTML" => 49 "PDF" => 11 ] ] "en" => array:12 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original Article</span>" "titulo" => "Endophthalmitis related to lemon allergy in a heroin addict" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "472" "paginaFinal" => "474" ] ] "contieneResumen" => array:1 [ "en" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 1291 "Ancho" => 2720 "Tamanyo" => 336976 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">IgE-western blot with allergic patient's serum. MW, molecular weight standard; lane 1, lemon pulp; lane 2, lemon peel; lane 3, lemon seeds; lane 4, Candida sp; lane 5, Penicillium sp; lane 6, Mucor sp; lane 7, Aspergillus sp.; lane 8, Alternaria alternate.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "A. Armentia, F. Pineda, B. Martin-Armentia, C. Ramos, F.J. Gil Martin, R. Palacios" "autores" => array:6 [ 0 => array:2 [ "nombre" => "A." "apellidos" => "Armentia" ] 1 => array:2 [ "nombre" => "F." "apellidos" => "Pineda" ] 2 => array:2 [ "nombre" => "B." "apellidos" => "Martin-Armentia" ] 3 => array:2 [ "nombre" => "C." "apellidos" => "Ramos" ] 4 => array:2 [ "nombre" => "F.J." "apellidos" => "Gil Martin" ] 5 => array:2 [ "nombre" => "R." "apellidos" => "Palacios" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0301054616300143?idApp=UINPBA00004N" "url" => "/03010546/0000004400000005/v1_201609110107/S0301054616300143/v1_201609110107/en/main.assets" ] "itemAnterior" => array:18 [ "pii" => "S0301054616300441" "issn" => "03010546" "doi" => "10.1016/j.aller.2016.03.002" "estado" => "S300" "fechaPublicacion" => "2016-09-01" "aid" => "755" "copyright" => "SEICAP" "documento" => "article" "crossmark" => 1 "subdocumento" => "fla" "cita" => "Allergol Immunopathol (Madr). 2016;44:461-6" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:2 [ "total" => 84 "formatos" => array:3 [ "EPUB" => 3 "HTML" => 60 "PDF" => 21 ] ] "en" => array:12 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original Article</span>" "titulo" => "Awareness of allergic enterocolitis among primary-care paediatricians: A web-based pilot survey" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "461" "paginaFinal" => "466" ] ] "contieneResumen" => array:1 [ "en" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0015" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 912 "Ancho" => 1576 "Tamanyo" => 68666 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Physicians who made the diagnosis of FPIES in the cohort of children cared by survey respondents.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "P. Comberiati, M. Landi, A. Martelli, G.L. Piacentini, C. Capristo, G. Paiola, D.G. Peroni" "autores" => array:7 [ 0 => array:2 [ "nombre" => "P." "apellidos" => "Comberiati" ] 1 => array:2 [ "nombre" => "M." "apellidos" => "Landi" ] 2 => array:2 [ "nombre" => "A." "apellidos" => "Martelli" ] 3 => array:2 [ "nombre" => "G.L." "apellidos" => "Piacentini" ] 4 => array:2 [ "nombre" => "C." "apellidos" => "Capristo" ] 5 => array:2 [ "nombre" => "G." "apellidos" => "Paiola" ] 6 => array:2 [ "nombre" => "D.G." "apellidos" => "Peroni" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0301054616300441?idApp=UINPBA00004N" "url" => "/03010546/0000004400000005/v1_201609110107/S0301054616300441/v1_201609110107/en/main.assets" ] "en" => array:19 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original Article</span>" "titulo" => "Increased prevalence of <span class="elsevierStyleItalic">Mycoplasma pneumoniae</span> serological positivity in Chilean young children" "tieneTextoCompleto" => true "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "467" "paginaFinal" => "471" ] ] "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "J. Carcey, P. Garcia, O. Padilla, J.A. Castro-Rodriguez" "autores" => array:4 [ 0 => array:3 [ "nombre" => "J." "apellidos" => "Carcey" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 1 => array:3 [ "nombre" => "P." "apellidos" => "Garcia" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] ] ] 2 => array:3 [ "nombre" => "O." "apellidos" => "Padilla" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] ] ] 3 => array:4 [ "nombre" => "J.A." "apellidos" => "Castro-Rodriguez" "email" => array:1 [ 0 => "jacastro17@hotmail.com" ] "referencia" => array:3 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] 2 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">*</span>" "identificador" => "cor0005" ] ] ] ] "afiliaciones" => array:3 [ 0 => array:3 [ "entidad" => "Division of Pediatrics, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile" "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Microbiology Unit, Clinical Service Laboratories Red Salud Christus-UC, Pontificia Universidad Católica de Chile, Santiago, Chile" "etiqueta" => "b" "identificador" => "aff0010" ] 2 => array:3 [ "entidad" => "Division of Public Health, School of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile" "etiqueta" => "c" "identificador" => "aff0015" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0015" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 1008 "Ancho" => 1659 "Tamanyo" => 94418 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Percentage of <span class="elsevierStyleItalic">M. pneumoniae</span> IgM seropositivity by month of the year during the 10-year study period (2004–2013).</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Mycoplasma pneumoniae</span> is a common cause of respiratory infections in children, and is recognised as having been one of the aetiologies of atypical pneumonia over the last 50 years.<a class="elsevierStyleCrossRefs" href="#bib0100"><span class="elsevierStyleSup">1,2</span></a> In children, <span class="elsevierStyleItalic">M. pneumoniae</span> is responsible for 10%–40% of all cases of community-acquired pneumonia.<a class="elsevierStyleCrossRefs" href="#bib0105"><span class="elsevierStyleSup">2,3</span></a> Although the infection is mild and self-limiting, some patients of all ages can develop severe and fulminating disease. <span class="elsevierStyleItalic">M. pneumoniae</span> can also manifest with extra-pulmonary symptoms.<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">4</span></a> Since there are no specific clinical symptoms or signs of help in diagnosing community-acquired pneumonia caused by this agent, epidemiological suspicion proves very important.<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">5</span></a> The early diagnosis of this microorganism allows specific antimicrobial therapy and limits its spread within the community.<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">6</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">Up until the early 1990s it was thought that <span class="elsevierStyleItalic">M. pneumoniae</span> was a pathogen that causes pneumonia in patients over five years of age. Since then, however, specialised diagnostic techniques have shown that <span class="elsevierStyleItalic">M. pneumoniae</span> may play an important role as a cause of upper and lower respiratory tract infections also in children under five years of age.<a class="elsevierStyleCrossRefs" href="#bib0105"><span class="elsevierStyleSup">2,7</span></a> A study has shown that in the last 16 years, the average age of <span class="elsevierStyleItalic">M. pneumoniae</span> seropositivity in patients with prolonged respiratory infections has decreased from 7 years to 2.9 years.<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">8</span></a> The more recent outbreaks in Korea have shown a peak in the early years of life in comparison to the situation found in the past.<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">9</span></a> Another study has reported the peak incidence of <span class="elsevierStyleItalic">M. pneumoniae</span> pneumonia in children to be in the 4–6 years age range.<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">2</span></a> Furthermore, the prevalence of <span class="elsevierStyleItalic">M. pneumoniae</span> pneumonia among hospitalised children under five years of age has been found to be 17%.<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">10</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">All these changes in the prevalence of <span class="elsevierStyleItalic">M. pneumoniae</span> should have inferences in the current treatment guidelines. However, there is little information on the relationship between this infection and age distribution in Latin America,<a class="elsevierStyleCrossRefs" href="#bib0135"><span class="elsevierStyleSup">8,11</span></a> and no previous epidemiological studies have been made in the concrete case of Chile. A single study conducted in ambulatory clinics in Santiago (Chile) reported a 2% prevalence of <span class="elsevierStyleItalic">M. pneumoniae</span> pharyngeal carrier status in 185 asymptomatic children.<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">6</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">The aim of this study is to determine the variation in prevalence over the last 10 years of <span class="elsevierStyleItalic">M. pneumoniae</span> IgM seropositivity according to age, particularly in pre-schoolers. Our working hypothesis is that seropositivity in children under five years of age has increased over the last 10 years in Santiago (Chile).</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Methods</span><p id="par0025" class="elsevierStylePara elsevierViewall">A cross-sectional study was made between 2004 and 2013 in our clinical university laboratory (UC-Christus C.M. San Joaquin, Santiago, Chile). Inclusion criteria: all samples collected for <span class="elsevierStyleItalic">M. pneumoniae</span> IgM serological testing requested by the attending physician were analysed. A browser was added with the program Query Builder (v. 6.0.7.0.0) to highlight all the results of <span class="elsevierStyleItalic">M. pneumoniae</span> IgM serological testing performed during this period. The result of such serological testing was considered to be positive based on the cut-off value used in our laboratory employing enzyme immunoassay (ELISA IgM M. pneumoniae Test System<span class="elsevierStyleSup">®</span>, Zeus Scientific, Raritan, NJ, USA) testing during 2004–2008, or indirect immunofluorescence (IFI M. pneumoniae IgM IFI Antibody Test System<span class="elsevierStyleSup">®</span>, Zeus Scientific, Raritan, NJ, USA) during 2009–2013. The sensitivity and specificity of these techniques were 89.1% and 92.8% for the ELISA, and 100% and 97.5%, respectively, for the IFI. The IFI technique was reported positivity with a cut-off point >1/16 dilutions, and indeterminate when the solution could not be clearly defined as either positive or negative.</p><p id="par0030" class="elsevierStylePara elsevierViewall">The following patient parameters were analysed: age, gender, year and month of processing of the test, and the origin of the test sample (ambulatory or in-hospital). For the purposes of this study, the results were stratified according to five age groups: patients under 5 years of age, 5–18 years of age, 19–50 years of age, 51–70 years of age, and over 70 years of age.</p><p id="par0035" class="elsevierStylePara elsevierViewall">The Ethics Committee of the Pontificia Universidad Catolica de Chile approved the study protocol (# 13-052).</p><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Statistical analysis</span><p id="par0040" class="elsevierStylePara elsevierViewall">In assessing the differences between positive versus negative <span class="elsevierStyleItalic">M. pneumoniae</span> serology, the chi-squared test was used for categorical variables and the Student <span class="elsevierStyleItalic">t</span>-test for continuous variables. A <span class="elsevierStyleItalic">p</span>-value of <0.05 using a two-tailed test was taken to indicate significance. Logistic regression analysis was performed to discard confounding variables in the analysis of IgM seropositivity over time. The SPSS<span class="elsevierStyleSup">®</span> version 17.0 statistical package (IBM, Armonk, NY, USA) was used throughout.</p></span></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0055">Results</span><p id="par0045" class="elsevierStylePara elsevierViewall">During the 10-year study period, a total of 20,020<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">M. pneumoniae</span> serological tests were retrieved from the database (54.3% corresponding to tests in females). Ambulatory patients were more frequently involved (83.2%) than hospitalised patients. A total of 29.4% (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>5886) of the study sample corresponded to children under the age of five years; while 35.6% (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>3824) corresponded to children between 5 and 18 years of age; 19.1% (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>7127) to patients between 19 and 50 years of age; 10.4% (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>2082) to patients between 51 and 70 years of age; and 5.4% (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>1081) to patients >70 years of age. The largest annual number of requested tests corresponded to the year 2011 with 2866 tests (13.3%) and the lowest to the year 2004 with 1481 tests (7.4%).</p><p id="par0050" class="elsevierStylePara elsevierViewall">Of the total serological tests, 31.9% yielded a positive result (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>6385), while 64.5% proved negative (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>12,915) and 3.6% were indeterminate (<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>720). The age group with the highest percentage <span class="elsevierStyleItalic">M. pneumoniae</span> seropositivity was the 5–18 years age group (45.6%), followed by the patients under five years of age (31.4%). The lowest percentage seropositivity corresponded to patients over 70 years of age (7.4%). From the age of 18 years to the oldest patients, percentage seropositivity was found to decrease with increasing age (from 24.3% to 7.4%).</p><p id="par0055" class="elsevierStylePara elsevierViewall">Over the 10-year study period, all age groups showed an increase in percentage seropositivity, although the two groups with the highest increments were the 5–18 years age group (from 15.8% to 54%), followed by the patients under five years of age (from 8.6% to 30%) (<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.001) (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>). Among the children under five years of age, those over two years of age showed a greater increase than those under two years of age (33% versus 17%, respectively; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.001). The group with the lowest increment corresponded to the patients over 70 years of age.</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0060" class="elsevierStylePara elsevierViewall">In all the age groups women showed the highest percentage seropositivity (<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05), except in the 51–70 years age group, where no significant gender differences were observed (<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.47) (<a class="elsevierStyleCrossRef" href="#fig0010">Fig. 2</a>).</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia><p id="par0065" class="elsevierStylePara elsevierViewall">As regards seasonal distribution in the course of the year, increased seropositivity was recorded in May, June, August, September and October. In general terms, a seropositivity increment of 0.5% monthly was observed over the years (<a class="elsevierStyleCrossRef" href="#fig0015">Fig. 3</a>).</p><elsevierMultimedia ident="fig0015"></elsevierMultimedia></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0060">Discussion</span><p id="par0070" class="elsevierStylePara elsevierViewall">The present study recorded a constant increase in the prevalence of positive <span class="elsevierStyleItalic">M. pneumoniae</span> serological tests in all age groups over the course of the 10-year study period. Following the patients between 5 and 18 years of age, the second highest increment corresponded to those younger than five years of age (particularly infants between 2 and 4 years of age).</p><p id="par0075" class="elsevierStylePara elsevierViewall">A number of factors may explain these findings, such as a progressive increase in the incidence of infections caused by this microorganism or an increase in clinical suspicion on the part of physicians over time. An important observation supporting this latter explanation is the fact that the requests for serological testing increased on a constant basis year-to-year from 2004 to 2013. Another possible explanation is that the new virological diagnostic techniques (e.g., molecular PCR) would cause the request for <span class="elsevierStyleItalic">M. pneumoniae</span> serological testing to be made in more selected patients than in the past.</p><p id="par0080" class="elsevierStylePara elsevierViewall">On the other hand, our results support the change in the classical conception that <span class="elsevierStyleItalic">M. pneumoniae</span> is infrequent in the first years of life.<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">7</span></a> The cause of the observed increase among children under the age of five years may be the more frequent existence of pre-school nurseries.<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">8</span></a> Accordingly, changes in therapy for respiratory infections in patients under the age of five years should be taken into account.<a class="elsevierStyleCrossRefs" href="#bib0110"><span class="elsevierStyleSup">3,8,10</span></a> A recent nationwide surveillance in Taiwan reports that children young than five years of age with community-acquired mycoplasma pneumonia have a significantly longer hospitalisation, higher intensive care unit admission rates and more complications than older children.<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">11</span></a></p><p id="par0085" class="elsevierStylePara elsevierViewall">In our study the greatest frequency of infection by <span class="elsevierStyleItalic">M. pneumoniae</span> corresponded to the female gender in patients less than 50 years of age. This is consistent with the literature, which describes an increase in frequency in this gender between 25 and 40 years of age.<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">3</span></a> The larger percentage of ambulatory origin can be interpreted as resulting from the fact that handling and suspicion of this infection occur mainly in the ambulatory care setting. However, due to the characteristics of the study, it is not known whether these patients needed further hospitalisation.</p><p id="par0090" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">M. pneumoniae</span> is endemic all over the world, at any time and in any season, although it is a little more frequent in the Northern hemisphere towards the end of summer and the beginning of autumn. Furthermore, outbreaks are common and occur in cycles of 4–7 years.<a class="elsevierStyleCrossRefs" href="#bib0110"><span class="elsevierStyleSup">3,4,12,13</span></a> In Chile (located in the Southern hemisphere) there was an increase in positive test results in May and June (autumn), follow by August, September and October (end of winter and early spring). These latter months have not been frequently cited.<a class="elsevierStyleCrossRefs" href="#bib0110"><span class="elsevierStyleSup">3,13</span></a> The prevalence of all clinical syndromes caused by <span class="elsevierStyleItalic">M. pneumoniae</span> varies from 2% during an endemic year to 35%–10% in epidemic periods.<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">3</span></a> In our study we observed an increase in percentage seropositivity in the year 2011 (from 20.5% to 60% in the period 2005–2011) that could be interpreted as an outbreak – although this can only be corroborated by follow-up data. Interestingly, in 2011 there was an increase also in England, Wales and Finland (e.g., Finland registered a 4-fold increment from 2005 to 2011).<a class="elsevierStyleCrossRefs" href="#bib0165"><span class="elsevierStyleSup">14,15</span></a></p><p id="par0095" class="elsevierStylePara elsevierViewall">A strong point of our study is the great number of tests performed in the same laboratory during these 10 years. However, the study also has some weaknesses. Firstly, obtaining the results referred only to the test caused a loss of information as to whether the seropositive results had any clinical repercussion (e.g., severity or need for treatment). Secondly, this is a single-centre study, and the results therefore cannot be generalised. On the other hand, two different laboratory techniques were used for <span class="elsevierStyleItalic">M. pneumoniae</span> testing (ELISA and IFI), although their sensitivity and specificity were similar. In turn, it is not known whether one same patient had two <span class="elsevierStyleItalic">M. pneumoniae</span> IgM samples during the acute infection episode (since negative conversion may take some time). Lastly, it is known that serological tests are difficult of interpret in the case of acute infection due to <span class="elsevierStyleItalic">M. pneumoniae</span>, especially when based on a single sample. However, in children the sole presence of IgM antibodies is a good indicator of acute infection.<a class="elsevierStyleCrossRef" href="#bib0175"><span class="elsevierStyleSup">16</span></a> Currently a very useful but expensive and not very widespread technique for the diagnosis of <span class="elsevierStyleItalic">M. pneumoniae</span> infection is real time-PCR (RT-PCR) testing in respiratory fluid samples. A recent study found that during the acute phase of the disease, the detection of IgM antibodies in combination with RT-PCR allows for a precise and reliable diagnosis of <span class="elsevierStyleItalic">M. pneumoniae</span> infection in children.<a class="elsevierStyleCrossRef" href="#bib0180"><span class="elsevierStyleSup">17</span></a> Another new available technique is the Ribotest<span class="elsevierStyleSup">®</span> (a rapid antigen kit for the detection of <span class="elsevierStyleItalic">M. pneumoniae</span> ribosomal protein L7/L12 using immunochromatographic assay), however their sensitivity is only 60% compared with RT-PCR.<a class="elsevierStyleCrossRef" href="#bib0185"><span class="elsevierStyleSup">18</span></a> In contrast, the study of the protein MPN474 (which plays an important role in cytoadherence of the cells during <span class="elsevierStyleItalic">M. pneumoniae</span> infection) has a high sensitivity (around 90.7%).<a class="elsevierStyleCrossRef" href="#bib0190"><span class="elsevierStyleSup">19</span></a></p><p id="par0100" class="elsevierStylePara elsevierViewall">In conclusion, following the 5–18 years age group, children under five years of age have shown the greatest increase in <span class="elsevierStyleItalic">M. pneumoniae</span> seropositivity over the last 10 years. It is therefore important for clinicians to suspect infection due to <span class="elsevierStyleItalic">M. pneumoniae</span> in this age group in order to ensure adequate diagnosis and treatment.</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Ethical disclosures</span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Confidentiality of data</span><p id="par0105" class="elsevierStylePara elsevierViewall">The authors declare that they have followed the protocols of their work centre on the publication of patient data and that all the patients included in the study have received sufficient information and have given their informed consent in writing to participate in that study.</p></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Right to privacy and informed consent</span><p id="par0110" class="elsevierStylePara elsevierViewall">The authors declare that no patient data appears in this article.</p></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Protection of human subjects and animals in research</span><p id="par0115" class="elsevierStylePara elsevierViewall">The authors declare that the procedures followed were in accordance with the regulations of the responsible Clinical Research Ethics Committee and in accordance with those of the World Medical Association and the Helsinki Declaration.</p></span></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Source of funding</span><p id="par0120" class="elsevierStylePara elsevierViewall">None.</p></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Financial disclosure</span><p id="par0125" class="elsevierStylePara elsevierViewall">The authors have no financial relationships relevant to this article.</p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Conflicts of interest</span><p id="par0130" class="elsevierStylePara elsevierViewall">The authors declare that they have no conflicts of interest.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:12 [ 0 => array:3 [ "identificador" => "xres729363" "titulo" => "Abstract" "secciones" => array:5 [ 0 => array:2 [ "identificador" => "abst0005" "titulo" => "Background" ] 1 => array:2 [ "identificador" => "abst0010" "titulo" => "Objective" ] 2 => array:2 [ "identificador" => "abst0015" "titulo" => "Method" ] 3 => array:2 [ "identificador" => "abst0020" "titulo" => "Results" ] 4 => array:2 [ "identificador" => "abst0025" "titulo" => "Conclusion" ] ] ] 1 => array:2 [ "identificador" => "xpalclavsec733418" "titulo" => "Keywords" ] 2 => array:2 [ "identificador" => "sec0005" "titulo" => "Introduction" ] 3 => array:3 [ "identificador" => "sec0010" "titulo" => "Methods" "secciones" => array:1 [ 0 => array:2 [ "identificador" => "sec0015" "titulo" => "Statistical analysis" ] ] ] 4 => array:2 [ "identificador" => "sec0020" "titulo" => "Results" ] 5 => array:2 [ "identificador" => "sec0025" "titulo" => "Discussion" ] 6 => array:3 [ "identificador" => "sec0030" "titulo" => "Ethical disclosures" "secciones" => array:3 [ 0 => array:2 [ "identificador" => "sec0035" "titulo" => "Confidentiality of data" ] 1 => array:2 [ "identificador" => "sec0040" "titulo" => "Right to privacy and informed consent" ] 2 => array:2 [ "identificador" => "sec0045" "titulo" => "Protection of human subjects and animals in research" ] ] ] 7 => array:2 [ "identificador" => "sec0050" "titulo" => "Source of funding" ] 8 => array:2 [ "identificador" => "sec0055" "titulo" => "Financial disclosure" ] 9 => array:2 [ "identificador" => "sec0060" "titulo" => "Conflicts of interest" ] 10 => array:2 [ "identificador" => "xack242413" "titulo" => "Acknowledgments" ] 11 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2015-12-11" "fechaAceptado" => "2016-02-08" "PalabrasClave" => array:1 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec733418" "palabras" => array:8 [ 0 => "<span class="elsevierStyleItalic">Mycoplasma pneumonia</span>" 1 => "Serology" 2 => "Epidemiology" 3 => "Prevalence" 4 => "Pre-schoolers" 5 => "Children" 6 => "IgM" 7 => "Latin America" ] ] ] ] "tieneResumen" => true "resumen" => array:1 [ "en" => array:3 [ "titulo" => "Abstract" "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Background</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">Mycoplasma pneumoniae</span> is a frequent cause of respiratory infections in school children and adolescents. Epidemiological suspicion is important, since there are no specific symptoms or signs to help in diagnosing infection caused by this agent.</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Objective</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">To determine the variation in prevalence over the last 10 years of <span class="elsevierStyleItalic">M. pneumoniae</span> IgM seropositivity according to age, particularly in pre-schoolers.</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Method</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">The results of <span class="elsevierStyleItalic">M. pneumoniae</span> IgM serological testing between January 2004 and December 2013 were analysed. Variables such as gender and month and year of sample processing were studied according to age groups (<5, 5–18, 19–50, 51–70 and >70 years of age).</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Results</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Of a total of 20,020 serological samples, 31.9% proved positive for <span class="elsevierStyleItalic">M. pneumoniae</span>. All age groups showed increases in percentage seropositivity over the last 10 years, although the most significant increase corresponded to the 5–18 years group (from 15.8% to 54%), followed by children <5 years of age (from 8.6% to 30%). Seropositivity was significantly higher in women in all age groups, except in those over 50 years of age.</p></span> <span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Conclusion</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">Children under five years of age were the group with the second highest increase in seropositivity.</p></span>" "secciones" => array:5 [ 0 => array:2 [ "identificador" => "abst0005" "titulo" => "Background" ] 1 => array:2 [ "identificador" => "abst0010" "titulo" => "Objective" ] 2 => array:2 [ "identificador" => "abst0015" "titulo" => "Method" ] 3 => array:2 [ "identificador" => "abst0020" "titulo" => "Results" ] 4 => array:2 [ "identificador" => "abst0025" "titulo" => "Conclusion" ] ] ] ] "multimedia" => array:3 [ 0 => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 1453 "Ancho" => 1584 "Tamanyo" => 117344 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Percentage of <span class="elsevierStyleItalic">M. pneumoniae</span> IgM seropositivity per year by age group during the 10-year study period (2003–2014). **<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0.001 for trend, between the results of the different age groups in relation to <5 years of age.</p>" ] ] 1 => array:7 [ "identificador" => "fig0010" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 1389 "Ancho" => 1570 "Tamanyo" => 78142 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Percentage of <span class="elsevierStyleItalic">M. pneumoniae</span> IgM seropositivity by gender during the 10-year study period (2004–2013).</p>" ] ] 2 => array:7 [ "identificador" => "fig0015" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 1008 "Ancho" => 1659 "Tamanyo" => 94418 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Percentage of <span class="elsevierStyleItalic">M. pneumoniae</span> IgM seropositivity by month of the year during the 10-year study period (2004–2013).</p>" ] ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:19 [ 0 => array:3 [ "identificador" => "bib0100" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Caracteri¿sticas cli¿nicas de la enfermedad respiratoria causada por <span class="elsevierStyleItalic">Mycoplasma pneumoniae</span> en nin¿os hospitalizeds" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "M.A. Paul" 1 => "L. 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Year/Month | Html | Total | |
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2024 November | 8 | 0 | 8 |
2024 October | 47 | 17 | 64 |
2024 September | 67 | 15 | 82 |
2024 August | 52 | 15 | 67 |
2024 July | 56 | 15 | 71 |
2024 June | 39 | 6 | 45 |
2024 May | 44 | 4 | 48 |
2024 April | 50 | 5 | 55 |
2024 March | 104 | 21 | 125 |
2024 February | 119 | 9 | 128 |
2024 January | 142 | 15 | 157 |
2023 December | 245 | 27 | 272 |
2023 November | 149 | 12 | 161 |
2023 October | 111 | 27 | 138 |
2023 September | 76 | 3 | 79 |
2023 August | 46 | 12 | 58 |
2023 July | 68 | 9 | 77 |
2023 June | 53 | 9 | 62 |
2023 May | 101 | 14 | 115 |
2023 April | 76 | 9 | 85 |
2023 March | 64 | 1 | 65 |
2023 February | 49 | 6 | 55 |
2023 January | 46 | 4 | 50 |
2022 December | 33 | 13 | 46 |
2022 November | 83 | 11 | 94 |
2022 October | 61 | 10 | 71 |
2022 September | 124 | 14 | 138 |
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2022 June | 36 | 6 | 42 |
2022 May | 52 | 7 | 59 |
2022 April | 40 | 6 | 46 |
2022 March | 27 | 10 | 37 |
2022 February | 20 | 5 | 25 |
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2021 December | 17 | 9 | 26 |
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2021 October | 20 | 12 | 32 |
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2021 April | 25 | 13 | 38 |
2021 March | 33 | 15 | 48 |
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2020 December | 1 | 0 | 1 |
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2020 July | 0 | 1 | 1 |
2018 February | 4 | 2 | 6 |
2018 January | 8 | 0 | 8 |
2017 December | 4 | 1 | 5 |
2017 November | 10 | 4 | 14 |
2017 October | 7 | 0 | 7 |
2017 September | 3 | 3 | 6 |
2017 August | 8 | 3 | 11 |
2017 July | 6 | 4 | 10 |
2016 October | 1 | 0 | 1 |
2016 September | 3 | 4 | 7 |