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Se muestra un nervio del estroma de la córnea del grupo B; se observa como una estructura lineal de alta reflexión con porciones gruesas y angostas (señaladas por las flechas) rodeada por queratocitos.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "M. Ramírez Fernández, E. Hernández Quintela, R. Naranjo Tackman" "autores" => array:3 [ 0 => array:2 [ "nombre" => "M." "apellidos" => "Ramírez Fernández" ] 1 => array:2 [ "nombre" => "E." "apellidos" => "Hernández Quintela" ] 2 => array:2 [ "nombre" => "R." 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Linear scan through the fovea. Thickening and loss of continuity of photoreceptor integrity line (arrow).</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "V.M. Asensio-Sánchez, L. Gonzalez-Buendia, M. Marcos-Fernández" "autores" => array:3 [ 0 => array:2 [ "nombre" => "V.M." "apellidos" => "Asensio-Sánchez" ] 1 => array:2 [ "nombre" => "L." "apellidos" => "Gonzalez-Buendia" ] 2 => array:2 [ "nombre" => "M." 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Marking with Indian ink. Flat-mounted cornea technique.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "L.C. Figueroa-Ortiz, O. Martín Rodríguez, A. García-Ben, J. García-Campos" "autores" => array:4 [ 0 => array:2 [ "nombre" => "L.C." "apellidos" => "Figueroa-Ortiz" ] 1 => array:2 [ "nombre" => "O." "apellidos" => "Martín Rodríguez" ] 2 => array:2 [ "nombre" => "A." "apellidos" => "García-Ben" ] 3 => array:2 [ "nombre" => "J." 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Ramírez Fernández, E. Hernández Quintela, R. Naranjo Tackman" "autores" => array:3 [ 0 => array:4 [ "nombre" => "M." "apellidos" => "Ramírez Fernández" "email" => array:2 [ 0 => "mramirez-f@prodigy.net.mx" 1 => "mramirezfdz@gmail.com" ] "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">*</span>" "identificador" => "cor0005" ] ] ] 1 => array:2 [ "nombre" => "E." "apellidos" => "Hernández Quintela" ] 2 => array:2 [ "nombre" => "R." "apellidos" => "Naranjo Tackman" ] ] "afiliaciones" => array:1 [ 0 => array:2 [ "entidad" => "Servicio de Córnea y Cirugía Refractiva, Asociación Para Evitar la Ceguera en México (APEC), Hospital Luis Sánchez Bulnes, Universidad Nacional Autónoma de México, Mexico City, Mexico" "identificador" => "aff0005" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "<span class="elsevierStyleItalic">Corresponding author</span>." ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Análisis de nervios estromales en pacientes con queratocono" ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0020" "etiqueta" => "Fig. 4" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr4.jpeg" "Alto" => 872 "Ancho" => 1314 "Tamanyo" => 42526 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0060" class="elsevierStyleSimplePara elsevierViewall">Mean<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>standard deviation of corneal stroma nerve thickness of group A, normal subjects, and group B, keratoconus patients. Difference in thickness between the two groups was statistically significant (<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05, unpaired <span class="elsevierStyleItalic">t</span> test).</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Corneal confocal microscopy under normal conditions reveals epithelium, subepithelial nerve plexus (the name given to the nerve plexus beneath cornea epithelium), stroma, stroma nerves and endothelium.<a class="elsevierStyleCrossRef" href="#bib0005"><span class="elsevierStyleSup">1</span></a> Regarding nerves, there have been many studies on the subepithelial nerve plexus of the cornea thanks to the ease of imaging on this plexus by confocal microscopy, including studies in keratoconus patients.<a class="elsevierStyleCrossRefs" href="#bib0010"><span class="elsevierStyleSup">2–6</span></a> However, corneal stroma nerves are much less abundant, and it is more difficult to take their images by confocal microscopy; therefore, the study was more limited.<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1,7,8</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">Although corneal stroma nerves are not as abundant as those in the subepithelial nerve plexus, upon usual eye examination by slit lamp, due to their size, corneal stroma nerves are the only ones that can be seen; for years they have been described as more apparent and thicker in keratoconus patients than in normal patients or those without this condition<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">9</span></a>; it has even been suggested that corneal stroma nerves are linked to keratoconus progression.<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">10</span></a> This study aims to take images and thus be able to compare as many nerves in the corneal stroma, between those from normal subjects and those from keratoconus patients, using confocal microscopy.</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Subjects, material and methods</span><p id="par0015" class="elsevierStylePara elsevierViewall">All people involved signed the consent form; the study was divided into two groups: group A, control group where 140 eyes of 70 normal subjects without any ocular or systemic disease were studied; and group B where 122 eyes of 87 patients diagnosed with keratoconus using topography (Bausch & Lomb Surgical, Orbtek Inc., Salt Lake City, UT, USA) in stages <span class="elsevierStyleSmallCaps">ii</span> and <span class="elsevierStyleSmallCaps">iii</span> based on the Amsler-Krumeich, classification, which has been used in multiple keratoconus studies.<a class="elsevierStyleCrossRefs" href="#bib0055"><span class="elsevierStyleSup">11–14</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">Confocal microscopy: At the corneal imaging unit of the Cornea and Refractive Surgery Department of the hospital of the Association to Prevent Blindness in Mexico, after topical anesthesia of cornea with tetracaine hydrochloride 5.0<span class="elsevierStyleHsp" style=""></span>mg per ml (Ponti Ofteno, Laboratorios Sophia, S.A. Guadalajara, Mexico), all study subjects in both groups underwent a central scan of the total cornea thickness using Confoscan 4 confocal microscope (Fortune Technologies, Vigonza, Italy). Each confocal microscopy test rendered scanned images in JPEG format, consisting of 2 consecutive scans of total central cornea thickness depth; this scan is equivalent to the endothelium and epithelium and back to endothelium imaging scan, i.e., from posterior to anterior and back to posterior, to allow movement in the <span class="elsevierStyleItalic">Z</span> axis of central cornea thickness. A Z-Ring Scan (Confoscan, Fortune Technologies, Italy) was used; this device maintains contact with the cornea surface to obtain reliable thickness measurements without anteroposterior eyeball movement.</p><p id="par0025" class="elsevierStylePara elsevierViewall">An average of 350 images per scan were obtained; they were 340<span class="elsevierStyleHsp" style=""></span>μm<span class="elsevierStyleHsp" style=""></span>×<span class="elsevierStyleHsp" style=""></span>255<span class="elsevierStyleHsp" style=""></span>μm at axes <span class="elsevierStyleItalic"><span class="elsevierStyleSmallCaps">X</span></span>, <span class="elsevierStyleItalic">Y</span>; they are automatically saved to a computer hard disk for further analysis using Navis v. 3.5.0 microscopic image analysis software (NIDEK, Multi-Instrument Diagnostic System, Japan).</p><p id="par0030" class="elsevierStylePara elsevierViewall">Each image from every scan obtained by confocal cornea microscopy was checked by searching for those with pictures of nerves in stroma. Only nerves in focus and with sharp edges were assessed and measured. Stroma nerve morphology and thickness were analyzed; they were measured using Navis v. 3.5.0 microscopic image analysis software (NIDEK, Multi-Instrument Diagnostic System, Japan). Nerve thickness was obtained from the average of the thickest and narrowest portion of each nerve tested (<a class="elsevierStyleCrossRefs" href="#fig0005">Figs. 1 and 2</a>). Nerves with bifurcations (<a class="elsevierStyleCrossRef" href="#fig0015">Fig. 3</a>) were not measured. Only corneal stroma nerves were analyzed. Subepithelial nerve plexus nerves were not measured.</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><elsevierMultimedia ident="fig0010"></elsevierMultimedia><elsevierMultimedia ident="fig0015"></elsevierMultimedia></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Results</span><p id="par0035" class="elsevierStylePara elsevierViewall">All confocal and central cornea thickness microscopy scans were integral, given that epithelium, subepithelial nerve plexus, endothelium, and corneal stroma were visible with keratocyte nuclei, starting with the first image beneath the subepithelial nerve plexus, up to the image above the corneal endothelium.</p><p id="par0040" class="elsevierStylePara elsevierViewall">A total of 243 stroma nerves in focus and with well-defined edges were obtained for analysis from all confocal microscopy scan images; 127 were from group A (subjects without pathology), and 116 from group B (keratoconus patients). All stroma nerves were observed in cornea confocal microscopy as well-defined linear and highly reflective structures, surrounded by corneal stroma keratocytes, the latter with moderate reflection (<a class="elsevierStyleCrossRefs" href="#fig0005">Figs. 1 and 2</a>). Corneal stroma nerves were arranged in an oblique direction in 100% of all images assessed, also in all cases with coarse and narrow morphology in some portions (<a class="elsevierStyleCrossRefs" href="#fig0005">Figs. 1 and 2</a>).</p><p id="par0045" class="elsevierStylePara elsevierViewall">Mean stroma nerve thickness in group A, i.e. patients without disease (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>) was 5.7<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>1.7 ranging from 3.3 to 10.4<span class="elsevierStyleHsp" style=""></span>μ, mean stroma nerves thickness in group B, keratoconus patients (<a class="elsevierStyleCrossRef" href="#fig0010">Fig. 2</a>) was 7.2<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>1.9 ranging from 3.5 to 12.0<span class="elsevierStyleHsp" style=""></span>μm. The difference in the thickness of corneal stroma nerves between group A and group B was statistically significant (<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05, unpaired <span class="elsevierStyleItalic">t</span> test) (<a class="elsevierStyleCrossRef" href="#fig0020">Fig. 4</a>).</p><elsevierMultimedia ident="fig0020"></elsevierMultimedia></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Discussion</span><p id="par0050" class="elsevierStylePara elsevierViewall">Under normal conditions, various structures such as corneal surface epithelium, basal epithelial cells, nerve plexus subepithelial, cores stromal keratocytes and endothelial cells may be assessed by confocal microscopy.<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1,7</span></a> Stroma nerves are no exception. In this study, they show as highly reflective linear structures with thick and thin portions, as already reported in the literature,<a class="elsevierStyleCrossRefs" href="#bib0075"><span class="elsevierStyleSup">15,16</span></a> surrounded by keratinocytes nuclei with moderate reflection.<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1,17</span></a></p><p id="par0055" class="elsevierStylePara elsevierViewall">Most studies of corneal nerves using confocal microscopy have focused mainly on subepithelial nerve plexus because it is across the cornea, beneath its epithelium, which makes it very easy to take images of the plexus.<a class="elsevierStyleCrossRefs" href="#bib0015"><span class="elsevierStyleSup">3,4,18</span></a> Therefore, there have been studies of the subepithelial nerve plexus under normal conditions<a class="elsevierStyleCrossRefs" href="#bib0015"><span class="elsevierStyleSup">3,19</span></a> (or changes the nerve plexus undergoes resulting from surgical procedures such as corneal transplantation<a class="elsevierStyleCrossRef" href="#bib0010"><span class="elsevierStyleSup">2</span></a> or refractive surgery<a class="elsevierStyleCrossRefs" href="#bib0020"><span class="elsevierStyleSup">4,20,21</span></a>) and diseases such as dry eye.<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">16</span></a> Subepithelial nerve plexus studies have been conducted on keratoconus patients, including linking them to corneal sensitivity.<a class="elsevierStyleCrossRefs" href="#bib0025"><span class="elsevierStyleSup">5,6</span></a> Furthermore, it is harder to find corneal stroma nerves, since it is not a plexus,<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">22</span></a> and given that images by confocal microscopy are 340<span class="elsevierStyleHsp" style=""></span>μm<span class="elsevierStyleHsp" style=""></span>×<span class="elsevierStyleHsp" style=""></span>255<span class="elsevierStyleHsp" style=""></span>μm in the axes <span class="elsevierStyleItalic"><span class="elsevierStyleSmallCaps">X</span></span>, <span class="elsevierStyleItalic">Y</span> (Confoscan 4 Fortune Technologies, Vigonza, Italy), it has to match the path of a corneal stroma nerve with the tiny image taken in the scan. This study focused on corneal stroma nerves.</p><p id="par0060" class="elsevierStylePara elsevierViewall">Keratoconus is a disease in which the cornea undergoes a usually progressive ectasia, which may appear in childhood or later in life and often leads to the need for a corneal transplant to correct the problem.<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">23</span></a> Early diagnosis of keratoconus today is of utmost importance due to the rise of refractive surgery, since it is a major contraindications for it.<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">24</span></a> Development and evolution of corneal topography equipment have allowed the diagnosis of keratoconus at increasingly early stages.<a class="elsevierStyleCrossRefs" href="#bib0125"><span class="elsevierStyleSup">25–30</span></a> However, for years it has been shown that there are subtle details such as observing corneal stroma nerves, most evident in keratoconus patients undergoing ophthalmic examination by slit lamp, which could prove incipient keratoconus<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">9</span></a> and thus support diagnosis in cases only suspected via corneal topography. There is even speculation on the role corneal nerves may have on keratoconus progression.<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">10</span></a> Even Al-Alqaba et al.<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">31</span></a> reported a case series of 14 corneal buttons disease in keratoconus patients, comparing them to 6 corneal buttons in patients without this condition, where the corneal stroma nerves were thicker. This study was performed to compare the thickness of corneal stroma nerves by confocal microscopy in vivo and in a larger group.</p><p id="par0065" class="elsevierStylePara elsevierViewall">Studies have been conducted using confocal microscopy in corneal stroma keratoconus patients, mainly focused on changes in density and reflection of stromal keratocytes, due to their easy capture by confocal microscopy since keratinocytes are found across stroma thickness.<a class="elsevierStyleCrossRefs" href="#bib0160"><span class="elsevierStyleSup">32,33</span></a> This study aimed to compare morphological characteristics and thickness of corneal stroma nerves using confocal microscopy, between normal subjects and patients diagnosed with keratoconus. We found no morphological differences between the two groups; both showed corneal stroma nerves and well-defined highly reflective linear structures, surrounded by keratinocytes as shown in confocal microscopy images of corneal stroma; they were arranged in an oblique direction in 100% of all images assessed, with various degrees of tortuosity of the thick and narrow portions in both groups. We did find a statistically significant difference regarding thickness of corneal stroma nerves; it was thicker in patients diagnosed with keratoconus, which underpins the classical idea that corneal stroma nerves are more apparent in ophthalmic examination by slit lamp in keratoconus patients.<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">9</span></a></p><p id="par0070" class="elsevierStylePara elsevierViewall">Corneal stroma nerves thickness has been studied in other diseases where they are known to be abnormally thick, such as the Mocan et al. study,<a class="elsevierStyleCrossRef" href="#bib0085"><span class="elsevierStyleSup">17</span></a> in which mean corneal stroma thickness in diabetic patients was 8.99<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>2.32<span class="elsevierStyleHsp" style=""></span>μm. In that study, 35 eyes were assessed in the group of patients with diabetes and 24 in the healthy subject control group; this study assessed 122 in the keratoconics group, and 140 in the control group without disease, and, even so, the latter shows corneal stroma nerve thickness of 5.7<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>1.7<span class="elsevierStyleHsp" style=""></span>μm, similar to the control group reported by Mocan et al. of 5.69<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>2.32<span class="elsevierStyleHsp" style=""></span>μm.<a class="elsevierStyleCrossRef" href="#bib0085"><span class="elsevierStyleSup">17</span></a></p><p id="par0075" class="elsevierStylePara elsevierViewall">In this study, stromal nerve with bifurcations were not measured, to avoid overestimating their thickness or altering the actual mean thickness of measured nerves.</p><p id="par0080" class="elsevierStylePara elsevierViewall">Only patients with a diagnosis of keratoconus in stages <span class="elsevierStyleSmallCaps">ii</span> and <span class="elsevierStyleSmallCaps">iii</span> based on the Amsler-Krumeich classification were included because stage <span class="elsevierStyleSmallCaps">iv</span> patients already have scarring that limits the taking and acquisition of quality corneal stroma images using confocal microscopy. Likewise, for stage <span class="elsevierStyleSmallCaps">i</span> patients, keratoconus diagnosis may be doubtful, and bias the study by measuring nerves in non-keratoconus patients, as we needed to be certain to measure corneal stroma nerves in patients with unquestionable keratoconus diagnosis.</p><p id="par0085" class="elsevierStylePara elsevierViewall">Thanks to confocal microscopy it is possible to evaluate corneal stroma nerves; although they are more difficult to capture in confocal microscopy images than subepithelial nerve plexus, a higher number of patients will yield enough images for analysis and measurement.</p><p id="par0090" class="elsevierStylePara elsevierViewall">This study can confirm that keratoconus patients have corneal stroma nerves thicker than those of normal subjects, and the aforementioned notion that, under ophthalmologic examination with slit lamp, corneal stroma nerves are more evident in keratoconus patients than in those without the disease.<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">9</span></a> The latter may provide additional data on clinical suspicion of keratoconus.</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Funding</span><p id="par0095" class="elsevierStylePara elsevierViewall">APEC, <span class="elsevierStyleSmallCaps">I</span>.A.P., Mexico City, Mexico.</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Conflicts of interest</span><p id="par0100" class="elsevierStylePara elsevierViewall">The authors declare that they have no conflicts of interest.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:11 [ 0 => array:2 [ "identificador" => "xres373921" "titulo" => array:5 [ 0 => "Abstract" 1 => "Objective" 2 => "Material and methods" 3 => "Results" 4 => "Conclusion" ] ] 1 => array:2 [ "identificador" => "xpalclavsec352983" "titulo" => "Keywords" ] 2 => array:2 [ "identificador" => "xres373922" "titulo" => array:5 [ 0 => "Resumen" 1 => "Objetivo" 2 => "Métodos" 3 => "Resultados" 4 => "Conclusiones" ] ] 3 => array:2 [ "identificador" => "xpalclavsec352984" "titulo" => "Palabras clave" ] 4 => array:2 [ "identificador" => "sec0005" "titulo" => "Introduction" ] 5 => array:2 [ "identificador" => "sec0010" "titulo" => "Subjects, material and methods" ] 6 => array:2 [ "identificador" => "sec0015" "titulo" => "Results" ] 7 => array:2 [ "identificador" => "sec0020" "titulo" => "Discussion" ] 8 => array:2 [ "identificador" => "sec0025" "titulo" => "Funding" ] 9 => array:2 [ "identificador" => "sec0030" "titulo" => "Conflicts of interest" ] 10 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2013-07-24" "fechaAceptado" => "2014-02-24" "PalabrasClave" => array:2 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec352983" "palabras" => array:5 [ 0 => "Cornea" 1 => "Stromal" 2 => "Keratoconus" 3 => "Nerves" 4 => "Confocal" ] ] ] "es" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec352984" "palabras" => array:5 [ 0 => "Córnea" 1 => "Estroma" 2 => "Queratocono" 3 => "Nervios" 4 => "Confocal" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "en" => array:2 [ "titulo" => "Abstract" "resumen" => "<span class="elsevierStyleSectionTitle" id="sect0010">Objective</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">To evaluate the differences in stromal corneal nerves between normal patients and keratoconus patients.</p> <span class="elsevierStyleSectionTitle" id="sect0015">Material and methods</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">A total of 140 eyes of 70 normal patients (group A) and 122 eyes of 87 keratoconus patients (group B) were examined with the confocal microscope, with a central scan of the total corneal thickness being taken. The morphology and thickness of the corneal stromal nerves were evaluated by using the Navis v. 3.5.0 software. Nerve thickness was obtained from the mean between the widest and the narrowest portions of each stromal nerve.</p> <span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Corneal stromal nerves were observed as irregular linear hyper-reflective structures with wide and narrow portions in all cases. Mean corneal stromal nerves thickness in group A was 5.7<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>1.7 (range from 3.3 to 10.4<span class="elsevierStyleHsp" style=""></span>μm), mean corneal stromal nerves thickness in group B was 7.2<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>1.9 (range from 3.5 to 12.0<span class="elsevierStyleHsp" style=""></span>μm). There was a statistical significant difference (<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>.05) in stromal corneal nerves thickness between group A and group B.</p> <span class="elsevierStyleSectionTitle" id="sect0025">Conclusion</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Stromal corneal nerves morphology was similar in both groups, but stromal nerves were thicker in keratoconus patients.</p>" ] "es" => array:2 [ "titulo" => "Resumen" "resumen" => "<span class="elsevierStyleSectionTitle" id="sect0035">Objetivo</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Evaluar las diferencias de los nervios del estroma de la córnea entre sujetos normales y pacientes con queratocono.</p> <span class="elsevierStyleSectionTitle" id="sect0040">Métodos</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Un total de 140 ojos de 70 sujetos normales (grupo A) y 122 ojos de 87 pacientes con queratocono (grupo B), fueron evaluados con el microscopio confocal, realizando un rastreo central del espesor total de la córnea. La morfología y el espesor de los nervios fueron evaluados utilizando el programa Navis v. 3.5.0. El espesor de los nervios se obtuvo del promedio de la porción más delgada y la más gruesa de cada nervio.</p> <span class="elsevierStyleSectionTitle" id="sect0045">Resultados</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Los nervios del estroma se observaron como estructuras lineales de alta reflexión e irregulares, con porciones gruesas y angostas en todos los casos. El promedio del espesor de los nervios en el grupo A fue de 5,7<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>1,7 (rango de 3,3 a 10,4<span class="elsevierStyleHsp" style=""></span>μ), en el grupo B fue de 7,2<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>1,9 (rango de 3,5 a 12,0<span class="elsevierStyleHsp" style=""></span>μ). La diferencia en el espesor de los nervios entre el grupo A y el grupo B fue estadísticamente significativa (p<span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0,05).</p> <span class="elsevierStyleSectionTitle" id="sect0050">Conclusiones</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">La morfología de los nervios del estroma de la córnea fue similar en ambos grupos; el espesor de estos fue mayor en los pacientes con queratocono.</p>" ] ] "NotaPie" => array:1 [ 0 => array:2 [ "etiqueta" => "☆" "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Please cite this article as: Ramírez Fernández M, Hernández Quintela E, Naranjo Tackman R. Análisis de nervios estromales en pacientes con queratocono. Arch Soc Esp Oftalmol. 2014;89:308–312.</p>" ] ] "multimedia" => array:4 [ 0 => array:7 [ "identificador" => "fig0005" "etiqueta" => "Fig. 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 713 "Ancho" => 975 "Tamanyo" => 144541 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Corneal stroma confocal microscopy image 340<span class="elsevierStyleHsp" style=""></span>μm<span class="elsevierStyleHsp" style=""></span>×<span class="elsevierStyleHsp" style=""></span>255<span class="elsevierStyleHsp" style=""></span>μm. Corneal stroma nerve of group A is shown, seen as a linear, highly reflective structure with thick and narrow portions (indicated by arrows) surrounded by keratinocytes.</p>" ] ] 1 => array:7 [ "identificador" => "fig0010" "etiqueta" => "Fig. 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 714 "Ancho" => 976 "Tamanyo" => 107152 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">Corneal stroma confocal microscopy image of 340<span class="elsevierStyleHsp" style=""></span>μm<span class="elsevierStyleHsp" style=""></span>×<span class="elsevierStyleHsp" style=""></span>255<span class="elsevierStyleHsp" style=""></span>μm. Corneal stroma nerve of group B is shown, seen as a linear, highly reflective structure with thick and narrow portions (indicated by arrows) surrounded by keratinocytes.</p>" ] ] 2 => array:7 [ "identificador" => "fig0015" "etiqueta" => "Fig. 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 713 "Ancho" => 975 "Tamanyo" => 114135 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">Corneal stroma confocal microscopy image of 340<span class="elsevierStyleHsp" style=""></span>μm<span class="elsevierStyleHsp" style=""></span>×<span class="elsevierStyleHsp" style=""></span>255<span class="elsevierStyleHsp" style=""></span>μm. Corneal stroma nerve bifurcation of group A is shown (indicated by arrows) surrounded by keratocytes.</p>" ] ] 3 => array:7 [ "identificador" => "fig0020" "etiqueta" => "Fig. 4" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr4.jpeg" "Alto" => 872 "Ancho" => 1314 "Tamanyo" => 42526 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0060" class="elsevierStyleSimplePara elsevierViewall">Mean<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>standard deviation of corneal stroma nerve thickness of group A, normal subjects, and group B, keratoconus patients. Difference in thickness between the two groups was statistically significant (<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span><<span class="elsevierStyleHsp" style=""></span>0.05, unpaired <span class="elsevierStyleItalic">t</span> test).</p>" ] ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:33 [ 0 => array:3 [ "identificador" => "bib0005" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Microscopia confocal de la córnea" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "V. Sanchez-Huerta" 1 => "M. Ramirez" 2 => "E. Hernandez-Quintela" 3 => "R. Naranjo Tackman" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Rev Mex Oftalmol" "fecha" => "2001" "volumen" => "75" "paginaInicial" => "57" "paginaFinal" => "61" ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib0010" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Keratocyte and subbasal nerve density after penetrating keratoplasty" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "S.V. Patel" 1 => "J.C. Erie" 2 => "J.W. McLaren" 3 => "W.M. Bourne" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Trans Am Ophthalmol Soc" "fecha" => "2007" "volumen" => "105" "paginaInicial" => "180" "paginaFinal" => "189" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18427608" "web" => "Medline" ] ] ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bib0015" "etiqueta" => "3" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Corneal subbasal nerve density: a comparison of two confocal microscopes" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "E.A. Erie" 1 => "J.W. McLaren" 2 => "K.M. Kittleson" 3 => "S.V. Patel" 4 => "J.C. Erie" 5 => "W.M. Bourne" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1097/ICL.0b013e31818b74f4" "Revista" => array:6 [ "tituloSerie" => "Eye Contact Lens" "fecha" => "2008" "volumen" => "34" "paginaInicial" => "322" "paginaFinal" => "325" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18997541" "web" => "Medline" ] ] ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bib0020" "etiqueta" => "4" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Reinnervation in the cornea after LASIK" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "B.H. Lee" 1 => "J.W. McLaren" 2 => "J.C. Erie" 3 => "D.O. Hodge" 4 => "W.M. Bourne" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Invest Ophthalmol Vis Sci" "fecha" => "2002" "volumen" => "43" "paginaInicial" => "3660" "paginaFinal" => "3664" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12454033" "web" => "Medline" ] ] ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bib0025" "etiqueta" => "5" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Mapping the corneal sub-basal nerve plexus in keratoconus by in vivo laser scanning confocal microscopy" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "D.V. Patel" 1 => "C.N. McGhee" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1167/iovs.05-1217" "Revista" => array:6 [ "tituloSerie" => "Invest Ophthalmol Vis Sci" "fecha" => "2006" "volumen" => "47" "paginaInicial" => "1348" "paginaFinal" => "1351" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16565367" "web" => "Medline" ] ] ] ] ] ] ] ] 5 => array:3 [ "identificador" => "bib0030" "etiqueta" => "6" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Laser scanning in vivo confocal microscopy and quantitative aesthesiometry reveal decreased corneal innervation and sensation in keratoconus" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "D.V. Patel" 1 => "J.Y. Ku" 2 => "R. Johnson" 3 => "C.N. McGhee" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Eye (Lond)" "fecha" => "2009" "volumen" => "23" "paginaInicial" => "586" "paginaFinal" => "592" ] ] ] ] ] ] 6 => array:3 [ "identificador" => "bib0035" "etiqueta" => "7" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Confocal microscopy in ophthalmology" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "J.C. Erie" 1 => "J.W. McLaren" 2 => "S.V. Patel" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.ajo.2009.06.022" "Revista" => array:6 [ "tituloSerie" => "Am J Ophthalmol" "fecha" => "2009" "volumen" => "148" "paginaInicial" => "639" "paginaFinal" => "646" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19674730" "web" => "Medline" ] ] ] ] ] ] ] ] 7 => array:3 [ "identificador" => "bib0040" "etiqueta" => "8" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Análisis de los nervios estromales de la córnea mediante microscopia confocal in vivo" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "M. Ramirez" 1 => "R. Naranjo-Tackman" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Rev Mex Oftalmol" "fecha" => "2011" "volumen" => "85" "paginaInicial" => "1" "paginaFinal" => "3" ] ] ] ] ] ] 8 => array:3 [ "identificador" => "bib0045" "etiqueta" => "9" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Keratoconus and related noninflammatory corneal thinning disorders" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "J.H. Krachmer" 1 => "R.S. Feder" 2 => "M.W. Belin" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Surv Ophthalmol" "fecha" => "1984" "volumen" => "28" "paginaInicial" => "293" "paginaFinal" => "322" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/6230745" "web" => "Medline" ] ] ] ] ] ] ] ] 9 => array:3 [ "identificador" => "bib0050" "etiqueta" => "10" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Involvement of corneal nerves in the progression of keratoconus" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "N.H. Brookes" 1 => "I.P. Loh" 2 => "G.M. Clover" 3 => "C.A. Poole" 4 => "T. Sherwin" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Exp Eye Res" "fecha" => "2003" "volumen" => "77" "paginaInicial" => "515" "paginaFinal" => "524" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12957150" "web" => "Medline" ] ] ] ] ] ] ] ] 10 => array:3 [ "identificador" => "bib0055" "etiqueta" => "11" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Evolution in the use of intrastromal corneal ring segments for corneal ectasia" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "J. Park" 1 => "D.C. Gritz" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1097/ICU.0b013e3283622a2c" "Revista" => array:6 [ "tituloSerie" => "Curr Opin Ophthalmol" "fecha" => "2013" "volumen" => "24" "paginaInicial" => "296" "paginaFinal" => "301" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23665526" "web" => "Medline" ] ] ] ] ] ] ] ] 11 => array:3 [ "identificador" => "bib0060" "etiqueta" => "12" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Correlation of corneal elevation with severity of keratoconus by means of anterior and posterior topographic analysis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "R. Ishii" 1 => "K. Kamiya" 2 => "A. Igarashi" 3 => "K. Shimizu" 4 => "Y. Utsumi" 5 => "T. Kumanomido" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1097/ICO.0B013E31823D1EE0" "Revista" => array:6 [ "tituloSerie" => "Cornea" "fecha" => "2012" "volumen" => "31" "paginaInicial" => "253" "paginaFinal" => "258" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22316650" "web" => "Medline" ] ] ] ] ] ] ] ] 12 => array:3 [ "identificador" => "bib0065" "etiqueta" => "13" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Intacs implantation using a femtosecond laser for management of keratoconus: comparison of 306 cases in different stages" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "A. Ertan" 1 => "G. Kamburoglu" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.jcrs.2008.05.028" "Revista" => array:6 [ "tituloSerie" => "J Cataract Refract Surg" "fecha" => "2008" "volumen" => "34" "paginaInicial" => "1521" "paginaFinal" => "1526" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18721713" "web" => "Medline" ] ] ] ] ] ] ] ] 13 => array:3 [ "identificador" => "bib0070" "etiqueta" => "14" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Biomechanical diagnosis of keratoconus: Evaluation of the keratoconus match index and the keratoconus match probability" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "G. Labiris" 1 => "Z. Gatzioufas" 2 => "H. Sideroudi" 3 => "A. Giarmoukakis" 4 => "V. Kozobolis" 5 => "B. Seitz" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/aos.12056" "Revista" => array:6 [ "tituloSerie" => "Acta Ophthalmol" "fecha" => "2013" "volumen" => "91" "paginaInicial" => "e258" "paginaFinal" => "e262" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23557430" "web" => "Medline" ] ] ] ] ] ] ] ] 14 => array:3 [ "identificador" => "bib0075" "etiqueta" => "15" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Corneal nerves alterations in various types of systemic polyneuropathy, identified by in vivo confocal microscopy" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "C. Zhao" 1 => "S. Lu" 2 => "A. Truffert" 3 => "N. Tajouri" 4 => "K. Zhao" 5 => "A. Mateo Montoya" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1055/s-2008-1027259" "Revista" => array:6 [ "tituloSerie" => "Klin Monbl Augenheilkd" "fecha" => "2008" "volumen" => "225" "paginaInicial" => "413" "paginaFinal" => "417" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18454383" "web" => "Medline" ] ] ] ] ] ] ] ] 15 => array:3 [ "identificador" => "bib0080" "etiqueta" => "16" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The córnea in Sjogren's syndrome: an in vivo confocal study" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "E. Villani" 1 => "D. Galimberti" 2 => "F. Viola" 3 => "C. Mapelli" 4 => "R. Ratiglia" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1167/iovs.06-1129" "Revista" => array:6 [ "tituloSerie" => "Invest Ophthalmol Vis Sci" "fecha" => "2007" "volumen" => "48" "paginaInicial" => "2017" "paginaFinal" => "2022" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17460255" "web" => "Medline" ] ] ] ] ] ] ] ] 16 => array:3 [ "identificador" => "bib0085" "etiqueta" => "17" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Morphologic alterations of both the stromal and subbasal nerves in the corneas of patients with diabetes" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "M.C. Mocan" 1 => "I. Durukan" 2 => "M. Irkec" 3 => "M. Orhan" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1097/01.ico.0000224640.58848.54" "Revista" => array:6 [ "tituloSerie" => "Cornea" "fecha" => "2006" "volumen" => "25" "paginaInicial" => "769" "paginaFinal" => "773" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17068451" "web" => "Medline" ] ] ] ] ] ] ] ] 17 => array:3 [ "identificador" => "bib0090" "etiqueta" => "18" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "An in vivo confocal masked study on corneal epithelium and subbasal nerves in patients with dry eye" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "J.M. Benitez del Castillo" 1 => "M.A. Wasfy" 2 => "C. Fernandez" 3 => "J. Garcia-Sanchez" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1167/iovs.04-0251" "Revista" => array:6 [ "tituloSerie" => "Invest Ophthalmol Vis Sci" "fecha" => "2004" "volumen" => "45" "paginaInicial" => "3030" "paginaFinal" => "3035" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15326117" "web" => "Medline" ] ] ] ] ] ] ] ] 18 => array:3 [ "identificador" => "bib0095" "etiqueta" => "19" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Epithelial innervation of human cornea: a three-dimensional study using confocal laser scanning fluorescence microscopy" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "R.F. Guthoff" 1 => "H. Wienss" 2 => "C. Hahnel" 3 => "A. Wree" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Cornea" "fecha" => "2005" "volumen" => "24" "paginaInicial" => "608" "paginaFinal" => "613" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15968170" "web" => "Medline" ] ] ] ] ] ] ] ] 19 => array:3 [ "identificador" => "bib0100" "etiqueta" => "20" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Comparison of corneal nerve regeneration and sensitivity between LASIK and laser epithelial keratomileusis (LASEK)" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "S.J. Lee" 1 => "J.K. Kim" 2 => "K.Y. Seo" 3 => "E.K. Kim" 4 => "H.K. Lee" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.ajo.2006.01.048" "Revista" => array:6 [ "tituloSerie" => "Am J Ophthalmol" "fecha" => "2006" "volumen" => "141" "paginaInicial" => "1009" "paginaFinal" => "1015" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16765667" "web" => "Medline" ] ] ] ] ] ] ] ] 20 => array:3 [ "identificador" => "bib0105" "etiqueta" => "21" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Confocal microscopy of corneal flap microfolds after LASIK" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "M. Ramirez" 1 => "E. Hernandez-Quintela" 2 => "V. Sanchez-Huerta" 3 => "R. Naranjo-Tackman" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "J Refract Surg" "fecha" => "2006" "volumen" => "22" "paginaInicial" => "155" "paginaFinal" => "158" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16523834" "web" => "Medline" ] ] ] ] ] ] ] ] 21 => array:3 [ "identificador" => "bib0110" "etiqueta" => "22" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Ultrastructural organization of human corneal nerves" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "L.J. Muller" 1 => "L. Pels" 2 => "G.F. Vrensen" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Invest Ophthalmol Vis Sci" "fecha" => "1996" "volumen" => "37" "paginaInicial" => "476" "paginaFinal" => "488" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8595948" "web" => "Medline" ] ] ] ] ] ] ] ] 22 => array:3 [ "identificador" => "bib0115" "etiqueta" => "23" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Clinical and epidemiological features of keratoconus genetic and external factors in the pathogenesis of the disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "A. Ihalainen" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Acta Ophthalmol Suppl" "fecha" => "1986" "volumen" => "178" "paginaInicial" => "1" "paginaFinal" => "64" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/3019073" "web" => "Medline" ] ] ] ] ] ] ] ] 23 => array:3 [ "identificador" => "bib0120" "etiqueta" => "24" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Topographic patterns in refractive surgery candidates" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "D. Varssano" 1 => "I. Kaiserman" 2 => "R. Hazarbassanov" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Cornea" "fecha" => "2004" "volumen" => "23" "paginaInicial" => "602" "paginaFinal" => "607" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15257000" "web" => "Medline" ] ] ] ] ] ] ] ] 24 => array:3 [ "identificador" => "bib0125" "etiqueta" => "25" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Corneal topography of early keratoconus" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "L.J. Maguire" 1 => "W.M. Bourne" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Am J Ophthalmol" "fecha" => "1989" "volumen" => "108" "paginaInicial" => "107" "paginaFinal" => "112" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/2757091" "web" => "Medline" ] ] ] ] ] ] ] ] 25 => array:3 [ "identificador" => "bib0130" "etiqueta" => "26" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Corneal topography" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "Y.S. Rabinowitz" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Curr Opin Ophthalmol" "fecha" => "1993" "volumen" => "4" "paginaInicial" => "68" "paginaFinal" => "74" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10148868" "web" => "Medline" ] ] ] ] ] ] ] ] 26 => array:3 [ "identificador" => "bib0135" "etiqueta" => "27" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Videokeratography of the fellow eye in unilateral keratoconus" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "Y.S. Rabinowitz" 1 => "A.B. Nesburn" 2 => "P.J. McDonnell" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Ophthalmology" "fecha" => "1993" "volumen" => "100" "paginaInicial" => "181" "paginaFinal" => "186" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8437824" "web" => "Medline" ] ] ] ] ] ] ] ] 27 => array:3 [ "identificador" => "bib0140" "etiqueta" => "28" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Keratoconus" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "Y.S. Rabinowitz" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Surv Ophthalmol" "fecha" => "1998" "volumen" => "42" "paginaInicial" => "297" "paginaFinal" => "319" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9493273" "web" => "Medline" ] ] ] ] ] ] ] ] 28 => array:3 [ "identificador" => "bib0145" "etiqueta" => "29" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Corneal elevation indices in normal and keratoconic eyes" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "H.B. Fam" 1 => "K.L. Lim" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.jcrs.2006.02.060" "Revista" => array:6 [ "tituloSerie" => "J Cataract Refract Surg" "fecha" => "2006" "volumen" => "32" "paginaInicial" => "1281" "paginaFinal" => "1287" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16863962" "web" => "Medline" ] ] ] ] ] ] ] ] 29 => array:3 [ "identificador" => "bib0150" "etiqueta" => "30" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Prevalence of Orbscan II corneal abnormalities in relatives of patients with keratoconus" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "T.M. Steele" 1 => "D.C. Fabinyi" 2 => "T.A. Couper" 3 => "M.S. Loughnan" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/j.1442-9071.2009.01908.x" "Revista" => array:6 [ "tituloSerie" => "Clin Experiment Ophthalmol" "fecha" => "2008" "volumen" => "36" "paginaInicial" => "824" "paginaFinal" => "830" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19278476" "web" => "Medline" ] ] ] ] ] ] ] ] 30 => array:3 [ "identificador" => "bib0155" "etiqueta" => "31" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The morphologic characteristics of corneal nerves in advanced keratoconus as evaluated by acetylcholinesterase technique" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "M.A. Al-Aqaba" 1 => "L. Faraj" 2 => "U. Fares" 3 => "A.M. Otri" 4 => "H.S. Dua" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.ajo.2011.03.006" "Revista" => array:6 [ "tituloSerie" => "Am J Ophthalmol" "fecha" => "2011" "volumen" => "152" "paginaInicial" => "364" "paginaFinal" => "376" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/21679914" "web" => "Medline" ] ] ] ] ] ] ] ] 31 => array:3 [ "identificador" => "bib0160" "etiqueta" => "32" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Keratocyte density in keratoconus. A confocal microscopy study(a)" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "J.C. Erie" 1 => "S.V. Patel" 2 => "J.W. McLaren" 3 => "C.B. Nau" 4 => "D.O. Hodge" 5 => "W.M. Bourne" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Am J Ophthalmol" "fecha" => "2002" "volumen" => "134" "paginaInicial" => "689" "paginaFinal" => "695" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12429244" "web" => "Medline" ] ] ] ] ] ] ] ] 32 => array:3 [ "identificador" => "bib0165" "etiqueta" => "33" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "In vivo confocal microscopy findings in keratoconus" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "O.O. Ucakhan" 1 => "A. Kanpolat" 2 => "N. Ylmaz" 3 => "M. Ozkan" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1097/01.icl.0000189038.74139.4a" "Revista" => array:6 [ "tituloSerie" => "Eye Contact Lens" "fecha" => "2006" "volumen" => "32" "paginaInicial" => "183" "paginaFinal" => "191" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16845264" "web" => "Medline" ] ] ] ] ] ] ] ] ] ] ] ] ] "idiomaDefecto" => "en" "url" => "/21735794/0000008900000008/v1_201410030031/S2173579414001571/v1_201410030031/en/main.assets" "Apartado" => array:4 [ "identificador" => "5816" "tipo" => "SECCION" "en" => array:2 [ "titulo" => "Original articles" "idiomaDefecto" => true ] "idiomaDefecto" => "en" ] "PDF" => "https://static.elsevier.es/multimedia/21735794/0000008900000008/v1_201410030031/S2173579414001571/v1_201410030031/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2173579414001571?idApp=UINPBA00004N" ]
Year/Month | Html | Total | |
---|---|---|---|
2018 February | 12 | 3 | 15 |
2018 January | 20 | 4 | 24 |
2017 December | 11 | 2 | 13 |
2017 November | 24 | 3 | 27 |
2017 October | 21 | 2 | 23 |
2017 September | 15 | 0 | 15 |
2017 August | 22 | 3 | 25 |
2017 July | 19 | 4 | 23 |
2017 June | 27 | 10 | 37 |
2017 May | 26 | 4 | 30 |
2017 April | 38 | 9 | 47 |
2017 March | 32 | 20 | 52 |
2017 February | 28 | 4 | 32 |
2017 January | 16 | 2 | 18 |
2016 December | 38 | 5 | 43 |
2016 November | 51 | 4 | 55 |
2016 October | 52 | 8 | 60 |
2016 September | 80 | 1 | 81 |
2016 August | 58 | 4 | 62 |
2016 July | 27 | 1 | 28 |
2016 June | 26 | 4 | 30 |
2016 May | 17 | 12 | 29 |
2016 April | 26 | 7 | 33 |
2016 March | 20 | 12 | 32 |
2016 February | 29 | 16 | 45 |
2016 January | 30 | 15 | 45 |
2015 December | 27 | 7 | 34 |
2015 November | 23 | 16 | 39 |
2015 October | 29 | 10 | 39 |
2015 September | 33 | 6 | 39 |
2015 August | 64 | 10 | 74 |
2015 June | 3 | 0 | 3 |
2015 May | 37 | 8 | 45 |
2015 April | 38 | 13 | 51 |
2015 March | 25 | 11 | 36 |
2015 February | 12 | 5 | 17 |
2015 January | 2 | 0 | 2 |
2014 November | 1 | 1 | 2 |