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Objetivo. Se trata de estudiar, según un modelo experimental, los efectos de la isquemia de 45 min sobre la microcirculación del hígado remanente a una resección hepática, así como de comprobar si dicha resección influye en la microcirculación del hígado no resecado durante el período de reperfusión. Material y método. Se han utilizado cuatro grupos de 8 ratas: grupo control, no sometido a isquemia prolongada; grupo control con resección del lóbulo hepático izquierdo; grupo sometido a isquemia de 45 min, y grupo sometido a isquemia de 45 min con resección del lóbulo hepático izquierdo. El efecto sobre la microcirculación se estudió mediante flujometría de láser-Doppler y estudio histológico.
Resultados. La isquemia de 45 min produce un descenso del flujo tisular hepático, en la primera hora de reperfusión, hasta del 35,48 y del 39,93% en los grupos con y sin resección asociada, respectivamente, sin diferencias entre ellos.
Conclusiones. La isquemia hepática de 45 min presenta diferencias en el flujo de la microcirculación posreperfusión, independientemente de haberse resecado o no el equivalente a un 40% del hígado.
Resultados. La isquemia de 45 min produce un descenso del flujo tisular hepático, en la primera hora de reperfusión, hasta del 35,48 y del 39,93% en los grupos con y sin resección asociada, respectivamente, sin diferencias entre ellos.
Conclusiones. La isquemia hepática de 45 min presenta diferencias en el flujo de la microcirculación posreperfusión, independientemente de haberse resecado o no el equivalente a un 40% del hígado.
Palabras clave:
Isquemia reperfusión-hepática
Microcirculación hepática
Láser-Doppler
Objective. The purpose of the present report was to carry out an experimental study of the effects of a 45-minute period of ischemia on the microcirculation of remnant liver following partial liver resection and to determine whether resection has any impact on the microcirculation of the nonresected liver during reperfusion.
Material and method. Four groups of eight rats each were used: a control group that was not subjected to prolonged ischemia; a control group that underwent resection of left liver lobe; a group subjected to 45 minutes of ischemia; and a group that underwent both a 45-minute period of ischemia and resection of left liver lobe. The effect on the microcirculation was assessed by means of laser-Doppler flowmetry and histological study.
Results. The 45-minute period of ischemia reduced the blood flow in the liver tissue during the first hour of reperfusion by as much as 35.48% and 39.43% in the groups with and without associated resection, respectively, rates that did not differ significantly from one to the other.
Conclusions.. The 45-minute period of liver ischemia changes postreperfusion blood flow through the hepatic microcirculation regardless of whether or not 40% of the liver has been resected.
Material and method. Four groups of eight rats each were used: a control group that was not subjected to prolonged ischemia; a control group that underwent resection of left liver lobe; a group subjected to 45 minutes of ischemia; and a group that underwent both a 45-minute period of ischemia and resection of left liver lobe. The effect on the microcirculation was assessed by means of laser-Doppler flowmetry and histological study.
Results. The 45-minute period of ischemia reduced the blood flow in the liver tissue during the first hour of reperfusion by as much as 35.48% and 39.43% in the groups with and without associated resection, respectively, rates that did not differ significantly from one to the other.
Conclusions.. The 45-minute period of liver ischemia changes postreperfusion blood flow through the hepatic microcirculation regardless of whether or not 40% of the liver has been resected.
Keywords:
Liver reperfusion ischemia
Hepatic microcirculation
Laser Doppler
