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Original article
Vitamin B12 in type 2 diabetic patients treated with metformin
Vitamina B12 en pacientes diabéticos tipo 2 en tratamiento con metformina
José María Calvo Romero
Corresponding author
jm.calvo@orangemail.es

Corresponding author.
, José Manuel Ramiro Lozano
Servicio de Medicina Interna, Hospital Ciudad de Coria, Coria, Cáceres, Spain
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    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Vitamin B<span class="elsevierStyleInf">12</span> deficiency is relatively common&#44; particularly in the elderly&#46;<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1&#44;2</span></a> Its clinical signs are mainly hematological and neuropsychiatric in nature&#44; and may sometimes be difficult to detect&#46; Vitamin B<span class="elsevierStyleInf">12</span> deficiency is usually due to vitamin malabsorption arising from various causes&#44; including the classical pernicious anemia &#40;PA&#41;&#46;<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1&#44;2</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">As far back as 1971 it was reported that 4 &#40;5&#46;6&#37;&#41; out of 71 diabetic patients on long-term treatment with metformin had low plasma vitamin B<span class="elsevierStyleInf">12</span> levels due to impaired absorption&#46;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> Various reports confirming this association have subsequently been published&#46; However&#44; to our knowledge &#40;and a search on MEDLINE&#41;&#44; no observational studies have been conducted in Spain comparing patients with type 2 diabetes treated and not treated with metformin&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Methods</span><p id="par0015" class="elsevierStylePara elsevierViewall">An observational&#44; cross-sectional study was conducted on consecutive patients with type 2 diabetes mellitus on drug treatment seen at two internal medicine clinics of a first level hospital in the north of the province of C&#225;ceres&#46; Plasma levels of vitamin B<span class="elsevierStyleInf">12</span> were measured in all patients&#46; Vitamin B<span class="elsevierStyleInf">12</span> deficiency was defined as levels less than 197<span class="elsevierStyleHsp" style=""></span>pg&#47;mL &#40;the lower limit of normal in our laboratory&#41;&#46; Patients with vitamin B<span class="elsevierStyleInf">12</span> deficiency were asked about their dietary habits and antiparietal cell and anti-intrinsic factor antibodies were measured&#46; An upper GI tract endoscopy was also performed to rule out atrophic chronic gastritis when it was considered indicated and with the patient&#39;s consent&#46; PA was diagnosed based on plasma vitamin B<span class="elsevierStyleInf">12</span> levels less than 197<span class="elsevierStyleHsp" style=""></span>pg&#47;mL&#44; the presence of anti-intrinsic factor antibodies and&#47;or atrophic chronic gastritis in gastric biopsies&#44; and the response to treatment with vitamin B<span class="elsevierStyleInf">12</span> in patients with hematological or neurological evidence of PA&#46;<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1&#44;2</span></a></p><p id="par0020" class="elsevierStylePara elsevierViewall">Statistical analysis was performed using a Chi-square test&#44; and a Fisher exact test when any of the expected values was less than 5&#44; to compare proportions&#44; and a Student&#39;s <span class="elsevierStyleItalic">t</span>-test for means comparison&#46; A value of <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05 was considered statistically significant&#46;</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Results</span><p id="par0025" class="elsevierStylePara elsevierViewall">The study sample consisted of 114 patients&#44; of whom 5 &#40;4&#46;4&#37;&#41; were excluded due to PA having been diagnosed&#46; The characteristics of patients treated and not treated with metformin are reported in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#46; Among the patients treated with metformin&#44; mean treatment time was 43&#46;5 months &#40;range 6&#8211;200 months&#41; and mean dose 1779<span class="elsevierStyleHsp" style=""></span>mg&#47;day &#40;range 425&#8211;2550<span class="elsevierStyleHsp" style=""></span>mg&#47;day&#41;&#46; Patients treated with metformin had significantly lower plasma vitamin B<span class="elsevierStyleInf">12</span> levels &#40;393&#46;5<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>184&#46;2 versus 509<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>176&#46;4<span class="elsevierStyleHsp" style=""></span>pg&#47;mL&#44; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;0008&#41;&#46; Seven &#40;8&#46;6&#37;&#41; of the 81 patients treated with metformin and none of the 28 patients given metformin had plasma vitamin B<span class="elsevierStyleInf">12</span> levels less than 197<span class="elsevierStyleHsp" style=""></span>pg&#47;mL&#46; No patient with vitamin B<span class="elsevierStyleInf">12</span> deficiency &#40;excluding those diagnosed with pernicious anemia&#41; had macrocytic anemia&#44; neuropathy&#44; or cognitive impairment&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0030" class="elsevierStylePara elsevierViewall">No correlation was found between metformin dose and plasma vitamin B<span class="elsevierStyleInf">12</span> levels &#40;<span class="elsevierStyleItalic">r</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#8722;0&#46;02&#44; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;45&#41; or between treatment time with metformin and plasma vitamin B<span class="elsevierStyleInf">12</span> levels &#40;<span class="elsevierStyleItalic">r</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;15&#44; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;78&#41;&#46; Among the patients treated with metformin&#44; there was no significant difference in plasma vitamin B<span class="elsevierStyleInf">12</span> levels between those taking and not taking a proton pump inhibitor &#40;PPI&#41; &#40;409&#46;4<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>205&#46;3 versus 385&#46;5<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>174&#46;1<span class="elsevierStyleHsp" style=""></span>pg&#47;mL&#44; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;58&#41;&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Discussion</span><p id="par0035" class="elsevierStylePara elsevierViewall">Metformin causes vitamin B<span class="elsevierStyleInf">12</span> malabsorption&#46;<a class="elsevierStyleCrossRefs" href="#bib0010"><span class="elsevierStyleSup">2&#44;3</span></a> The absorption of vitamin B<span class="elsevierStyleInf">12</span> bound to intrinsic factor produced from ileum is calcium-dependent&#46; Calcium in ileal lumen enhances uptake of the vitamin B<span class="elsevierStyleInf">12</span>-intrinsic factor complex by the ileal cell receptor&#44; and metformin impairs calcium availability at ileal level&#46;<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">4</span></a> Various studies&#44; including the current study&#44; support the association of metformin therapy with decreased plasma levels of vitamin B<span class="elsevierStyleInf">12</span>&#46; A study published in 1976 reported low plasma vitamin B<span class="elsevierStyleInf">12</span> levels in 5 &#40;16&#46;7&#37;&#41; out of 30 diabetic patients treated with metformin&#46;<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a> In another retrospective study&#44; patients treated with metformin had significantly lower plasma vitamin B<span class="elsevierStyleInf">12</span> levels &#40;496 versus 637<span class="elsevierStyleHsp" style=""></span>pg&#47;mL&#41;&#46;<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">6</span></a> In a large observational study&#44; 5&#46;8&#37; of diabetic patients over 50 years of age treated with metformin for a mean of 5 years had vitamin B<span class="elsevierStyleInf">12</span> deficiency&#44; as compared to 2&#46;4&#37; of diabetic patients not given metformin&#46;<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a> In a prospective study of patients with type 2 diabetes mellitus treated with insulin&#44; 9&#46;9&#37; of patients given metformin at doses of 2&#44;550<span class="elsevierStyleHsp" style=""></span>mg&#47;day for longer than 4 years had vitamin B<span class="elsevierStyleInf">12</span> levels less than 150<span class="elsevierStyleHsp" style=""></span>pmol&#47;L &#40;200<span class="elsevierStyleHsp" style=""></span>pg&#47;mL&#41;&#44; as compared to 2&#46;7&#37; of patients who received placebo&#46;<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">8</span></a> These results may be considered similar to those of the present study&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall">The effect of metformin in decreasing plasma vitamin B<span class="elsevierStyleInf">12</span> levels is not transient and appears to increase with treatment duration&#46;<a class="elsevierStyleCrossRefs" href="#bib0030"><span class="elsevierStyleSup">6&#44;8&#44;9</span></a> A progressive decrease over time &#40;during more than 4 years of follow-up&#41; in mean plasma vitamin B<span class="elsevierStyleInf">12</span> levels has been reported in patients treated with metformin&#46;<a class="elsevierStyleCrossRefs" href="#bib0030"><span class="elsevierStyleSup">6&#44;8</span></a> A case&#8211;control study showed an association between vitamin B<span class="elsevierStyleInf">12</span> deficiency and the dose and duration of metformin treatment&#46;<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">9</span></a> By contrast&#44; our study found no correlation between metformin treatment dose and duration and plasma vitamin B<span class="elsevierStyleInf">12</span> levels&#46; It should be noted&#44; however&#44; that short-term treatment with metformin already decreases vitamin B<span class="elsevierStyleInf">12</span> levels&#46; In a 6-month study&#44; treatment with metformin caused a mean 20<span class="elsevierStyleHsp" style=""></span>pg&#47;mL reduction in vitamin B<span class="elsevierStyleInf">12</span> levels&#46;<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">10</span></a> An additional 16-week study also showed a 14&#37; reduction in plasma vitamin B<span class="elsevierStyleInf">12</span> levels&#46;<a class="elsevierStyleCrossRef" href="#bib0055"><span class="elsevierStyleSup">11</span></a> The use of PPIs has also been associated with vitamin B<span class="elsevierStyleInf">12</span> deficiency&#46;<a class="elsevierStyleCrossRef" href="#bib0060"><span class="elsevierStyleSup">12</span></a> Acid secretion inhibition impairs vitamin B<span class="elsevierStyleInf">12</span> release from food&#46;<a class="elsevierStyleCrossRef" href="#bib0065"><span class="elsevierStyleSup">13</span></a> However&#44; our study found no negative impact of treatment with PPIs on plasma vitamin B<span class="elsevierStyleInf">12</span> levels&#46; This observation agrees with the findings in another study&#46;<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">9</span></a></p><p id="par0045" class="elsevierStylePara elsevierViewall">All the patients in this study with vitamin B<span class="elsevierStyleInf">12</span> deficiency associated with metformin had so-called &#8220;asymptomatic&#8221; deficiency &#40;defined as low plasma vitamin B<span class="elsevierStyleInf">12</span> levels with no associated macrocytic anemia&#44; neuropathy&#44; or cognitive impairment&#41;&#46;<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1&#44;2</span></a> However&#44; this deficiency is not always asymptomatic&#46; In a series of 10 patients with vitamin B<span class="elsevierStyleInf">12</span> deficiency associated with metformin&#44; 9 patients had mild anemia and 3 had peripheral neuropathy&#46;<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">14</span></a> Isolated cases of patients with symptomatic vitamin B<span class="elsevierStyleInf">12</span> deficiency associated with metformin have also been reported&#46;<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">15</span></a> There is no agreement as to whether patients with &#8220;asymptomatic&#8221; vitamin B<span class="elsevierStyleInf">12</span> deficiency should be treated&#46;<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1&#44;2</span></a> There are signs of vitamin B<span class="elsevierStyleInf">12</span> deficiency&#44; particularly neurological signs&#44; which are difficult to diagnose and may become irreversible&#46;<a class="elsevierStyleCrossRef" href="#bib0010"><span class="elsevierStyleSup">2</span></a> In addition&#44; vitamin B<span class="elsevierStyleInf">12</span> deficiency is invariably associated with elevated homocysteine levels<a class="elsevierStyleCrossRefs" href="#bib0040"><span class="elsevierStyleSup">8&#44;11</span></a> and their potentially harmful consequences&#46;<a class="elsevierStyleCrossRefs" href="#bib0080"><span class="elsevierStyleSup">16&#44;17</span></a> On the other hand&#44; one study did not show an improvement in cognitive function after vitamin B<span class="elsevierStyleInf">12</span> administration to elderly patients with mild vitamin B<span class="elsevierStyleInf">12</span> deficiency&#46;<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">18</span></a></p><p id="par0050" class="elsevierStylePara elsevierViewall">An additional concept&#44; functional vitamin B<span class="elsevierStyleInf">12</span> deficiency&#44; is defined as the presence of normal vitamin B<span class="elsevierStyleInf">12</span> levels together with increased plasma levels of methylmalonic acid and is based on the fact that vitamin B<span class="elsevierStyleInf">12</span> deficiency invariably causes elevated methylmalonic acid levels&#46;<a class="elsevierStyleCrossRefs" href="#bib0095"><span class="elsevierStyleSup">19&#8211;21</span></a> Such a functional deficiency has been associated with neuropathy and anemia&#46;<a class="elsevierStyleCrossRefs" href="#bib0095"><span class="elsevierStyleSup">19&#8211;21</span></a> An increased frequency of neuropathy has recently been reported in patients with type 2 diabetes mellitus and this functional deficiency&#44; as well as an improvement in neuropathy after the administration of vitamin B<span class="elsevierStyleInf">12</span> and the normalization of plasma levels of methylmalonic acid&#46;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a></p><p id="par0055" class="elsevierStylePara elsevierViewall">There is no agreement regarding the convenience of regular measurements of plasma vitamin B<span class="elsevierStyleInf">12</span> levels in patients treated with metformin&#46;<a class="elsevierStyleCrossRefs" href="#bib0040"><span class="elsevierStyleSup">8&#44;9&#44;14&#44;15&#44;22</span></a> Controversy also exists concerning the management of patients with vitamin B<span class="elsevierStyleInf">12</span> deficiency associated with metformin&#44; and some authors even suggest that metformin should be discontinued&#46; In agreement with other authors&#44;<a class="elsevierStyleCrossRefs" href="#bib0015"><span class="elsevierStyleSup">3&#44;8</span></a> we think it is appropriate to regularly measure plasma vitamin B<span class="elsevierStyleInf">12</span> levels in patients treated with metformin&#44; although other authors advise against such measurements&#46;<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">22</span></a> Moreover&#44; we think it is appropriate to continue treatment with metformin in patients with vitamin B<span class="elsevierStyleInf">12</span> deficiency and to treat such patients with oral or intramuscular vitamin B<span class="elsevierStyleInf">12</span> even if the deficiency is &#8220;asymptomatic&#8221;&#46; In our experience &#40;data not collected in the study&#41; and that of other authors&#44;<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">14</span></a> plasma vitamin B<span class="elsevierStyleInf">12</span> levels easily normalized after the administration of oral or intramuscular vitamin B<span class="elsevierStyleInf">12</span>&#46; This approach is based on the benefits of metformin for patients with type 2 diabetes mellitus and on the fact that treatment with vitamin B<span class="elsevierStyleInf">12</span> is simple&#44; inexpensive&#44; and safe and also has potential benefits&#46;<a class="elsevierStyleCrossRefs" href="#bib0005"><span class="elsevierStyleSup">1&#44;2</span></a> Oral calcium supplements may reverse vitamin B<span class="elsevierStyleInf">12</span> malabsorption induced by metformin&#44; and could be a treatment option&#46;<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">4</span></a></p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle">Conflicts of interest</span><p id="par0060" class="elsevierStylePara elsevierViewall">The authors state that they have no conflicts of interest&#46;</p></span></span>"
    "textoCompletoSecciones" => array:1 [
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          "identificador" => "xres148832"
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        1 => array:2 [
          "identificador" => "xpalclavsec136741"
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        2 => array:2 [
          "identificador" => "xres148833"
          "titulo" => array:5 [
            0 => "Resumen"
            1 => "Objetivo"
            2 => "M&#233;todos"
            3 => "Resultados"
            4 => "Conclusiones"
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        3 => array:2 [
          "identificador" => "xpalclavsec136740"
          "titulo" => "Palabras clave"
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        4 => array:2 [
          "identificador" => "sec0005"
          "titulo" => "Introduction"
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        5 => array:2 [
          "identificador" => "sec0010"
          "titulo" => "Methods"
        ]
        6 => array:2 [
          "identificador" => "sec0015"
          "titulo" => "Results"
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        7 => array:2 [
          "identificador" => "sec0020"
          "titulo" => "Discussion"
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        8 => array:2 [
          "identificador" => "sec0025"
          "titulo" => "Conflicts of interest"
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        9 => array:1 [
          "titulo" => "References"
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    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2012-04-11"
    "fechaAceptado" => "2012-06-11"
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        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Keywords"
          "identificador" => "xpalclavsec136741"
          "palabras" => array:4 [
            0 => "Cobalamin"
            1 => "Diabetes mellitus"
            2 => "Metformin"
            3 => "Vitamin B<span class="elsevierStyleInf">12</span>"
          ]
        ]
      ]
      "es" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palabras clave"
          "identificador" => "xpalclavsec136740"
          "palabras" => array:4 [
            0 => "Cobalamina"
            1 => "Diabetes mellitus"
            2 => "Metformina"
            3 => "Vitamina B<span class="elsevierStyleInf">12</span>"
          ]
        ]
      ]
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    "resumen" => array:2 [
      "en" => array:2 [
        "titulo" => "Abstract"
        "resumen" => "<span class="elsevierStyleSectionTitle">Objective</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">To test vitamin B<span class="elsevierStyleInf">12</span> plasma levels in type 2 diabetic patients treated with metformin in our area&#46;</p> <span class="elsevierStyleSectionTitle">Methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">A cross-sectional&#44; observational study of consecutive type 2 diabetic patients on drug treatment attending an internal medicine outpatient clinic&#46;</p> <span class="elsevierStyleSectionTitle">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">One hundred and nine patients &#40;81 treated with metformin&#41; were enrolled into the study&#46; Mean time on metformin treatment was 43&#46;5 months and mean drug dose was 1779<span class="elsevierStyleHsp" style=""></span>mg&#47;day&#46; Patients treated with metformin had significantly lower vitamin B<span class="elsevierStyleInf">12</span> plasma levels &#40;393&#46;5 vs 509<span class="elsevierStyleHsp" style=""></span>pg&#47;mL&#44; <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;0008&#41;&#46; Seven &#40;8&#46;6&#37;&#41; of 81 patients treated with metformin and none of the 28 patients not treated with the drug had vitamin B<span class="elsevierStyleInf">12</span> plasma levels lower than 197<span class="elsevierStyleHsp" style=""></span>pg&#47;mL&#46; No correlation was found between vitamin B<span class="elsevierStyleInf">12</span> plasma levels and metformin treatment time or dosage&#46;</p> <span class="elsevierStyleSectionTitle">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">In type 2 diabetic patients&#44; treatment with metformin is associated to lower vitamin B<span class="elsevierStyleInf">12</span> plasma levels&#46; Vitamin B<span class="elsevierStyleInf">12</span> deficiency associated with metformin is relatively common in our area&#46;</p>"
      ]
      "es" => array:2 [
        "titulo" => "Resumen"
        "resumen" => "<span class="elsevierStyleSectionTitle">Objetivo</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">Estudiar los niveles plasm&#225;ticos de vitamina B<span class="elsevierStyleInf">12</span> en pacientes diab&#233;ticos tipo 2 tratados con metformina en nuestro medio&#46;</p> <span class="elsevierStyleSectionTitle">M&#233;todos</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Estudio observacional transversal de pacientes consecutivos con diabetes mellitus tipo 2 en tratamiento farmacol&#243;gico atendidos en consulta de Medicina Interna&#46;</p> <span class="elsevierStyleSectionTitle">Resultados</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Se estudiaron 109 pacientes &#40;81 en tratamiento con metformina&#41;&#46; El tiempo medio en tratamiento con metformina fue 43&#44;5 meses y la dosis media fue 1&#46;779<span class="elsevierStyleHsp" style=""></span>mg&#47;d&#237;a&#46; Los pacientes tratados con metformina tuvieron unas concentraciones plasm&#225;ticas de vitamina B<span class="elsevierStyleInf">12</span> significativamente menores &#40;393&#44;5 frente a 509 pg&#47;ml&#44; p<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#44;0008&#41;&#46; Siete &#40;8&#44;6&#37;&#41; de 81 pacientes tratados con metformina y ninguno de los 28 no tratados con metformina tuvieron unas concentraciones plasm&#225;ticas inferiores a 197 pg&#47;ml&#46; No hubo correlaci&#243;n entre las concentraciones de vitamina B<span class="elsevierStyleInf">12</span> y el tiempo en tratamiento con metformina o la dosis de metformina&#46;</p> <span class="elsevierStyleSectionTitle">Conclusiones</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">En pacientes diab&#233;ticos tipo 2&#44; el tratamiento con metformina se asocia a concentraciones plasm&#225;ticas de vitamina B<span class="elsevierStyleInf">12</span> m&#225;s bajas&#46; El d&#233;ficit de vitamina B<span class="elsevierStyleInf">12</span> asociado a metformina es relativamente frecuente en nuestro medio&#46;</p>"
      ]
    ]
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        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara">Please cite this article as&#58; Calvo Romero JM&#44; Ramiro Lozano JM&#46; Vitamina B<span class="elsevierStyleInf">12</span> en pacientes diab&#233;ticos tipo 2 en tratamiento con metformina&#46; Endocrinol Nutr&#46; 2012&#59;59&#40;8&#41;&#58;487&#8211;90&#46;</p>"
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          "leyenda" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">HbA<span class="elsevierStyleInf">1c</span>&#58; glycosylated hemoglobin&#59; PPIs&#58; proton pump inhibitors&#59; BMI&#58; body mass index&#59; NS&#58; no statistically significant &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#8805;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#41;&#46;</p>"
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                  \t\t\t\t  " align="" valign="\n
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                  \t\t\t\t" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t" style="border-bottom: 2px solid black">With metformin &#40;n<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>81&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t" style="border-bottom: 2px solid black">Without metformin &#40;n<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>28&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">p</span>&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t">Age&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t">71&#46;6<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>12&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">75&#46;4<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>8&#46;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">NS&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t">Sex &#40;female&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t">56 &#40;69&#46;1&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t">16 &#40;57&#46;1&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t">NS&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t">BMI &#40;kg&#47;m<span class="elsevierStyleSup">2</span>&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t">30&#46;4<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>5&#46;4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t">29&#46;3<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>3&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">NS&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
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                  \t\t\t\t">Treatment with PPIs&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">27 &#40;33&#46;3&#37;&#41;&nbsp;\t\t\t\t\t\t\n
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      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0005"
          "bibliografiaReferencia" => array:22 [
            0 => array:3 [
              "identificador" => "bib0005"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "An update on cobalamin deficiency in adults"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "N&#46; Dali-Youcef"
                            1 => "E&#46; Andr&#232;s"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1093/qjmed/hcn138"
                      "Revista" => array:6 [
                        "tituloSerie" => "QJM"
                        "fecha" => "2009"
                        "volumen" => "102"
                        "paginaInicial" => "17"
                        "paginaFinal" => "28"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18990719"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0010"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Vitamin B-12 deficiency in the elderly&#58; current dilemmas"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "S&#46;P&#46; Stabler"
                            1 => "J&#46; Lindenbaum"
                            2 => "R&#46;H&#46; Allen"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Clin Nutr"
                        "fecha" => "1997"
                        "volumen" => "66"
                        "paginaInicial" => "741"
                        "paginaFinal" => "749"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9322547"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0015"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Vitamin-B12 status of patients on long-term metformin therapy"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "G&#46;H&#46; Tomkin"
                            1 => "D&#46;R&#46; Hadden"
                            2 => "J&#46;A&#46; Weaver"
                            3 => "D&#46;A&#46; Montgomery"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "BMJ"
                        "fecha" => "1971"
                        "volumen" => "2"
                        "paginaInicial" => "685"
                        "paginaFinal" => "687"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20792936"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib0020"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Increased intake of calcium reverses vitamin B12 malabsorption induced by metformin"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "W&#46;A&#46; Bauman"
                            1 => "S&#46; Shaw"
                            2 => "E&#46; Jayatilleke"
                            3 => "A&#46;M&#46; Spungen"
                            4 => "V&#46; Herbert"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Diabetes Care"
                        "fecha" => "2000"
                        "volumen" => "23"
                        "paginaInicial" => "1227"
                        "paginaFinal" => "1231"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10977010"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib0025"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Vitamin B 12 and folic acid serum levels in diabetics under various therapeutic regimens"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "J&#46;L&#46; Carpentier"
                            1 => "J&#46; Bury"
                            2 => "A&#46; Luyckx"
                            3 => "P&#46; Lefebvre"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Diabete Metab"
                        "fecha" => "1976"
                        "volumen" => "2"
                        "paginaInicial" => "187"
                        "paginaFinal" => "190"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/1017538"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib0030"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The effect of metformin therapy on vitamin D and B12 levels in patients with diabetes mellitus type 2"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "E&#46; Kos"
                            1 => "M&#46;J&#46; Liszek"
                            2 => "M&#46;A&#46; Emanuele"
                            3 => "R&#46; Durazo-Arvizu"
                            4 => "P&#46; Camacho"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.4158/EP11009.OR"
                      "Revista" => array:6 [
                        "tituloSerie" => "Endocr Pract"
                        "fecha" => "2012"
                        "volumen" => "18"
                        "paginaInicial" => "179"
                        "paginaFinal" => "184"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/21940283"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib0035"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Association of biochemical B12 deficiency with metformin therapy and vitamin B12 supplements&#58; the national health and nutrition examination survey&#44; 1999&#8211;2006"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "L&#46; Reinstatler"
                            1 => "Y&#46;P&#46; Qi"
                            2 => "R&#46;S&#46; Williamson"
                            3 => "J&#46;V&#46; Garn"
                            4 => "G&#46;P&#46; Oakley Jr&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.2337/dc11-1582"
                      "Revista" => array:6 [
                        "tituloSerie" => "Diabetes Care"
                        "fecha" => "2012"
                        "volumen" => "35"
                        "paginaInicial" => "327"
                        "paginaFinal" => "333"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22179958"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib0040"
              "etiqueta" => "8"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Long term treatment with metformin in patients with type 2 diabetes and risk of vitamin B-12 deficiency&#58; randomised placebo controlled trial"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "J&#46; de Jager"
                            1 => "A&#46; Kooy"
                            2 => "P&#46; Lehert"
                            3 => "M&#46;G&#46; Wulffel&#233;"
                            4 => "J&#46; van der Kolk"
                            5 => "D&#46; Bets"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "BMJ"
                        "fecha" => "2010"
                        "volumen" => "340"
                        "paginaInicial" => "c2181"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20488910"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib0045"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Risk factors of vitamin B&#40;12&#41; deficiency in patients receiving metformin"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "R&#46;Z&#46; Ting"
                            1 => "C&#46;C&#46; Szeto"
                            2 => "M&#46;H&#46; Chan"
                            3 => "K&#46;K&#46; Ma"
                            4 => "K&#46;M&#46; Chow"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Arch Internal Med"
                        "fecha" => "2006"
                        "volumen" => "166"
                        "paginaInicial" => "1975"
                        "paginaFinal" => "1979"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib0050"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Effects of metformin or rosiglitazone on serum concentrations of homocysteine&#44; folate&#44; and vitamin B12 in patients with type 2 diabetes mellitus"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "M&#46; Sahin"
                            1 => "N&#46;B&#46; Tutuncu"
                            2 => "D&#46; Ertugrul"
                            3 => "N&#46; Tanaci"
                            4 => "N&#46;D&#46; Guvener"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.jdiacomp.2005.10.005"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Diabetes Complications"
                        "fecha" => "2007"
                        "volumen" => "21"
                        "paginaInicial" => "118"
                        "paginaFinal" => "123"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17331860"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib0055"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Effects of short-term treatment with metformin on serum concentrations of homocysteine&#44; folate and vitamin B12 in type 2 diabetes mellitus&#58; a randomized&#44; placebo-controlled trial"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "M&#46;G&#46; Wulffel&#233;"
                            1 => "A&#46; Kooy"
                            2 => "P&#46; Lehert"
                            3 => "D&#46; Bets"
                            4 => "J&#46;C&#46; Ogterop"
                            5 => "B&#46; Borger van der Burg"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Internal Med"
                        "fecha" => "2003"
                        "volumen" => "254"
                        "paginaInicial" => "455"
                        "paginaFinal" => "463"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/14535967"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            11 => array:3 [
              "identificador" => "bib0060"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Effect of proton pump inhibitors on vitamins and iron"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "K&#46;E&#46; McColl"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1038/ajg.2008.135"
                      "Revista" => array:7 [
                        "tituloSerie" => "Am J Gastroenterol"
                        "fecha" => "2009"
                        "volumen" => "104"
                        "numero" => "Suppl&#46; 2"
                        "paginaInicial" => "5"
                        "paginaFinal" => "9"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19098839"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib0065"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Metformin and vitamin B12 deficiency&#58; the role of H2 receptor antagonists and proton pump inhibitors"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "G&#46;I&#46; Varughese"
                            1 => "J&#46;H&#46; Scarpello"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1093/ageing/afl139"
                      "Revista" => array:6 [
                        "tituloSerie" => "Age Ageing"
                        "fecha" => "2007"
                        "volumen" => "36"
                        "paginaInicial" => "110"
                        "paginaFinal" => "111"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17264140"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib0070"
              "etiqueta" => "14"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Metformin-associated vitamin B12 deficiency"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "E&#46; Andr&#232;s"
                            1 => "E&#46; Noel"
                            2 => "B&#46; Goichot"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Arch Internal Med"
                        "fecha" => "2002"
                        "volumen" => "162"
                        "paginaInicial" => "2251"
                        "paginaFinal" => "2252"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            14 => array:3 [
              "identificador" => "bib0075"
              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Metformin-related vitamin B12 deficiency"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "K&#46;W&#46; Liu"
                            1 => "L&#46;K&#46; Dai"
                            2 => "W&#46; Jean"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1093/ageing/afj042"
                      "Revista" => array:6 [
                        "tituloSerie" => "Age Ageing"
                        "fecha" => "2006"
                        "volumen" => "35"
                        "paginaInicial" => "200"
                        "paginaFinal" => "201"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16495296"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            15 => array:3 [
              "identificador" => "bib0080"
              "etiqueta" => "16"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The homocysteine controversy"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "Y&#46;M&#46; Smulders"
                            1 => "H&#46;J&#46; Blom"
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                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1007/s10545-010-9151-1"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Inherit Metab Dis"
                        "fecha" => "2011"
                        "volumen" => "34"
                        "paginaInicial" => "93"
                        "paginaFinal" => "99"
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                      ]
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                ]
              ]
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                      "titulo" => "Homocysteine in neurological disease&#58; a marker or a cause&#63;"
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                          "etal" => false
                          "autores" => array:4 [
                            0 => "S&#46; Khanna"
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                            2 => "K&#46;K&#46; Pillai"
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                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
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                        "tituloSerie" => "CNS Neurol Disord Drug Targets"
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                        "volumen" => "10"
                        "paginaInicial" => "361"
                        "paginaFinal" => "369"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20874694"
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                          ]
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                      ]
                    ]
                  ]
                ]
              ]
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              "etiqueta" => "18"
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                          "etal" => true
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                            5 => "P&#46;M&#46; Ueland"
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                      ]
                    ]
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                        "paginaInicial" => "361"
                        "paginaFinal" => "370"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16895884"
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                      ]
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                  ]
                ]
              ]
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              "etiqueta" => "19"
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                0 => array:2 [
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                    0 => array:2 [
                      "titulo" => "Disorders of cobalamin &#40;vitamin B12&#41; metabolism&#58; emerging concepts in pathophysiology&#44; diagnosis and treatment"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
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                    0 => array:2 [
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                        "tituloSerie" => "Blood Rev"
                        "fecha" => "2007"
                        "volumen" => "21"
                        "paginaInicial" => "113"
                        "paginaFinal" => "130"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16814909"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            19 => array:3 [
              "identificador" => "bib0100"
              "etiqueta" => "20"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Functional vitamin B12 deficiency"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "M&#46;R&#46; Turner"
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                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1136/jnnp.2008.161968"
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                        "tituloSerie" => "Pract Neurol"
                        "fecha" => "2009"
                        "volumen" => "9"
                        "paginaInicial" => "37"
                        "paginaFinal" => "41"
                        "link" => array:1 [
                          0 => array:2 [
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                      ]
                    ]
                  ]
                ]
              ]
            ]
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                0 => array:2 [
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                    0 => array:2 [
                      "titulo" => "Diabetes as a cause of clinically significant functional cobalamin deficiency"
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                          "autores" => array:1 [
                            0 => "L&#46;R&#46; Solomon"
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                    0 => array:2 [
                      "doi" => "10.2337/dc11-0009"
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                        "tituloSerie" => "Diabetes Care"
                        "fecha" => "2011"
                        "volumen" => "34"
                        "paginaInicial" => "1077"
                        "paginaFinal" => "1080"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/21421801"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
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                    0 => array:2 [
                      "titulo" => "Reduced serum vitamin B-12 in patients taking metformin"
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                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20488912"
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