Scientific letter
Possible intrauterine transmission of SARS-CoV-2: Ultrasound findings and viral molecular detection in amniotic fluid
Posible transmisión intrauterina del SARS-CoV-2: Hallazgos ecográficos y detección molecular viral en líquido amniótico
Maria Carolina Andrade Maiaa, Carolina Santos Souza Tavaresb, Cliomar Alves dos Santosc, Paulo Ricardo Martins-Filhob,
Corresponding author
a Division of Gynaecology and Obstetrics, University Hospital/EBSERH, Federal University of Sergipe, Aracaju, Brazil
b Investigative Pathology Laboratory, Federal University of Sergipe, Aracaju, Brazil
c Health Foundation Parreiras Horta, Central Laboratory of Public Health (LACEN/SE), Sergipe State Health Secretariat, Aracaju, Brazil
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"etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 1 => array:3 [ "nombre" => "Carolina Santos Souza" "apellidos" => "Tavares" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] ] ] 2 => array:3 [ "nombre" => "Cliomar Alves dos" "apellidos" => "Santos" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] ] ] 3 => array:4 [ "nombre" => "Paulo Ricardo" "apellidos" => "Martins-Filho" "email" => array:1 [ 0 => "prmartinsfh@gmail.com" ] "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">*</span>" "identificador" => "cor0005" ] ] ] ] "afiliaciones" => array:3 [ 0 => array:3 [ "entidad" => "Division of Gynaecology and Obstetrics, University Hospital/EBSERH, Federal University of Sergipe, Aracaju, Brazil" "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Investigative Pathology Laboratory, Federal University of Sergipe, Aracaju, Brazil" "etiqueta" => "b" "identificador" => "aff0010" ] 2 => array:3 [ "entidad" => "Health Foundation Parreiras Horta, Central Laboratory of Public Health (LACEN/SE), Sergipe State Health Secretariat, Aracaju, Brazil" "etiqueta" => "c" "identificador" => "aff0015" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Posible transmisión intrauterina del SARS-CoV-2: Hallazgos ecográficos y detección molecular viral en líquido amniótico" ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Fig. 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 774 "Ancho" => 2007 "Tamanyo" => 246414 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Ultrasound images at 25 weeks of gestation showing liver (A) and placental (B) calcifications. At 28 weeks of gestation, there was progression of calcifications and changes in placental shape (C). At 36 weeks of pregnancy, the patient presented amniochorial detachment (D). Two days after birth, the child had persistent calcifications in the right and left liver lobes (E).</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall">The coronavirus disease-19 (COVID-19) is an infectious disease associated to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel single-stranded RNA-enveloped virus primarily transmitted person-to-person by close contact through respiratory droplets. In addition, studies have shown the effects of SARS-CoV-2 infection during pregnancy including potential vertical transmission of the virus.<a class="elsevierStyleCrossRefs" href="#bib0055"><span class="elsevierStyleSup">1–3</span></a> Although the mechanisms of intrauterine transmission are not fully understood,<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">4</span></a> it has been found that SARS-CoV-2 infection may lead to placental vascular lesions, breakdown of the placental barrier and fetal intrauterine viremia.<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">5</span></a> In this rare report, we present fetal ultrasound (US) findings and RT-PCR results in a possible case of vertical transmission of SARS-CoV-2.</p><p id="par0010" class="elsevierStylePara elsevierViewall">We describe the case of a 42-year-old pregnant woman with past medical history of hypertension and gestational diabetes. At 11 and 20 weeks of gestational age, she had flu-like symptoms (runny nose, sore throat, cough, sneezing and headache) and the results of RT-PCR for SARS-CoV-2 were positive at both time points. At 25 weeks of gestation, US findings showed symmetric hyperechogenic signals (“in mirror”) in the right and left fetal hypochondrium suggesting liver calcifications, and placenta exhibiting punctate hyperechogenic foci also compatible with calcifications (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>A and B). At 28 weeks of gestation, there was a significant progression in calcifications and changes in placental shape (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>C). In the 32nd week, the pregnant woman was vaccinated with AstraZeneca/Oxford COVID-19 vaccine.</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0015" class="elsevierStylePara elsevierViewall">STORCH (syphilis, toxoplasmosis, rubella, cytomegalovirus, and herpes simplex virus) screening test results were negative. At 36 weeks of pregnancy, there was a reduction in fetal movements with amniochorial detachment (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>D), and the patient was referred for a cesarean delivery. On admission for delivery, the patient had no symptoms suggestive of COVID-19 and the RT-PCR result from nasopharyngeal swab was negative. A full-term female infant weighing 2238<span class="elsevierStyleHsp" style=""></span>kg was born uneventful with an Apgar score of 9 and 10 in 1 and 5<span class="elsevierStyleHsp" style=""></span>min, respectively. Intraoperative amniotic fluid was collected for RT-PCR analysis, which showed a positive result for SARS-CoV-2 (cycle threshold value: 30). On the 2nd postpartum day, nasopharyngeal swabs were collected from the mother and newborn and the RT-PCR results were negative. SARS-CoV-2 IgG antibodies were detected in neonatal blood serum two days after birth, but IgA results were negative. Intrahepatic calcifications continued to be found on ultrasound examination after birth (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>E). No clinical or neurological complications were documented during the first six months of life.</p><p id="par0020" class="elsevierStylePara elsevierViewall">Vertical transmission can be defined as the generational transmission of viruses from mothers to their offspring, whether intrauterine, intrapartum or in the early postnatal period. There is limited evidence on the vertical transmission of SARS-CoV-2. In a review of 51 studies with 336 infants, only one amniotic fluid sample was positive for SARS-CoV-2 by RT-PCR.<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">6</span></a> It is known that SARS-CoV-2 uses the angiotensin-converting enzyme 2 (ACE2) receptor for the entry into the host cells especially in kidneys, heart, respiratory and gastrointestinal tract tissues. In addition, previous studies have also shown the expression of ACE2 in the placenta.<a class="elsevierStyleCrossRef" href="#bib0085"><span class="elsevierStyleSup">7</span></a> Despite these findings, the potential mechanisms of maternal-fetal transmission of SARS-CoV-2 remain unclear. A recent study has found a high expression of ACE2 in the placenta during the first and second trimesters of pregnancy, which suggests a potential risk for intrauterine infection, placenta dysfunction, and pregnancy complications from SARS-CoV-2.<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">8</span></a> Contrasting results have been observed during the third trimester of pregnancy. In a prospective cohort study performed by Edlow et al.,<a class="elsevierStyleCrossRef" href="#bib0095"><span class="elsevierStyleSup">9</span></a> it was suggested a nonoverlapping placental ACE2 and TMPRSS2 (a vital serine protease for the SARS-CoV-2 infectivity) expression as a protective mechanism against vertical transmission of SARS-CoV-2 during the third trimester, despite the decreased transplacental transfer of anti–SARS-CoV-2 antibodies to the fetus.</p><p id="par0025" class="elsevierStylePara elsevierViewall">In this report, our patient had two positive results for SARS-CoV-2 infection during the first 20 weeks of pregnancy and placental changes in the following weeks. In addition, hepatomegaly and persistent calcifications were found in the fetus. Although intrahepatic calcifications are common findings in chromosomal disorders and infections,<a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">10</span></a> there was no diagnosis of aneuploidy or congenital malformation and the results for STORCH were negative. Furthermore, there is evidence of increased expression of ACE2 in liver fibroblasts and hepatocytes from first to second trimester, which suggests that fetal liver is a vulnerable target organ of SARS-COV-2.<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">8</span></a></p><p id="par0030" class="elsevierStylePara elsevierViewall">Our findings are suggestive of a possible <span class="elsevierStyleItalic">in utero</span> SARS-CoV-2 transmission, since there is evidence of maternal SARS-CoV-2 infection during the first weeks of pregnancy, placental and fetal changes suggestive of viral infection, and a positive RT-PCR result for the detection of SARS-CoV-2 RNA from a sterile sample (amniotic fluid) during childbirth. Although the child had no clinical or neurological complications after delivery, this case reinforces the possibility of vertical transmission of SARS-CoV-2 and the need for vaccination of pregnant women against COVID-19.</p><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0005">Authors’ contributions</span><p id="par0035" class="elsevierStylePara elsevierViewall">All authors contributed equally to the manuscript.</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Funding</span><p id="par0040" class="elsevierStylePara elsevierViewall">The authors declare no financial support.</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Conflict of interest</span><p id="par0045" class="elsevierStylePara elsevierViewall">The authors have no competing interests to declare.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:4 [ 0 => array:2 [ "identificador" => "sec0005" "titulo" => "Authors’ contributions" ] 1 => array:2 [ "identificador" => "sec0010" "titulo" => "Funding" ] 2 => array:2 [ "identificador" => "sec0015" "titulo" => "Conflict of interest" ] 3 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "multimedia" => array:1 [ 0 => array:7 [ "identificador" => "fig0005" "etiqueta" => "Fig. 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 774 "Ancho" => 2007 "Tamanyo" => 246414 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Ultrasound images at 25 weeks of gestation showing liver (A) and placental (B) calcifications. At 28 weeks of gestation, there was progression of calcifications and changes in placental shape (C). At 36 weeks of pregnancy, the patient presented amniochorial detachment (D). Two days after birth, the child had persistent calcifications in the right and left liver lobes (E).</p>" ] ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0015" "bibliografiaReferencia" => array:10 [ 0 => array:3 [ "identificador" => "bib0055" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Vertical transmission and clinical outcome of the neonates born to SARS-CoV-2-positive mothers: a tertiary care hospital-based observational study" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "P. Kumar" 1 => "Fadila" 2 => "A. Prasad" 3 => "A. Akhtar" 4 => "B.K. Chaudhary" 5 => "L.K. 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| Year/Month | Html | Total | |
|---|---|---|---|
| 2024 October | 18 | 8 | 26 |
| 2024 September | 41 | 3 | 44 |
| 2024 August | 31 | 10 | 41 |
| 2024 July | 32 | 13 | 45 |
| 2024 June | 21 | 13 | 34 |
| 2024 May | 17 | 20 | 37 |
| 2024 April | 18 | 63 | 81 |
| 2024 March | 32 | 42 | 74 |
| 2024 February | 44 | 21 | 65 |
| 2024 January | 19 | 18 | 37 |
| 2023 December | 36 | 19 | 55 |
| 2023 November | 52 | 29 | 81 |
| 2023 October | 53 | 47 | 100 |
| 2023 September | 26 | 14 | 40 |
| 2023 August | 16 | 0 | 16 |
| 2023 July | 27 | 45 | 72 |
| 2023 June | 14 | 6 | 20 |
| 2023 May | 9 | 4 | 13 |
| 2023 March | 1 | 0 | 1 |
| 2023 February | 2 | 16 | 18 |
| 2023 January | 2 | 4 | 6 |
| 2022 December | 13 | 10 | 23 |
| 2022 November | 0 | 2 | 2 |
| 2022 October | 0 | 1 | 1 |
| 2022 April | 0 | 2 | 2 |
| 2022 March | 0 | 8 | 8 |
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