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"documento" => "article" "crossmark" => 1 "subdocumento" => "rev" "cita" => "Gastroenterol Hepatol. 2020;43:34-45" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:2 [ "total" => 79 "formatos" => array:2 [ "HTML" => 48 "PDF" => 31 ] ] "en" => array:13 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Review</span>" "titulo" => "Donor-specific antibodies in liver transplantation" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => array:2 [ 0 => "en" 1 => "es" ] "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "34" "paginaFinal" => "45" ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Anticuerpos específicos del donante en el trasplante de hígado" ] ] "contieneResumen" => array:2 [ "en" => true "es" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 2638 "Ancho" => 2276 "Tamanyo" => 256054 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">(A) Complement-dependent cytotoxicity (CDC) assay. (1) A 56- (class I HLA) or 26-well (class II HLA) plate contains frozen living cells expressing HLA antigens representative of the HLA antigen distribution in the population of interest. (2) Patient serum is added to each well and donor-specific antibodies (DSA), if present, bind to the corresponding HLA antigens (<span class="elsevierStyleItalic">upper panel</span>: serum without DSA; <span class="elsevierStyleItalic">lower panel</span>: serum with DSA). (3) Non donor-specific antibodies are washed away, whereas DSA remain attached to their complementary HLA antigens. (4) Complement (blue star) and vital fluorescent dye are added to the wells, resulting in cell lysis through a membrane attack complex (yellow cylinder)-dependent mechanism only in patients with DSA; lysed cells are stained by the dye (viable cell in green, lysed cell in red). (5) The plate is finally read with a fluorescence microscope: in each well, the cell population is described as viable or dead; and the result is expressed as a panel reactive antibody (PRA) value, defined as the percentage of cells/wells in the panel that give a positive reaction (in our example, 5/56<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>9%). (B) Solid-phase assay (SPA). Fluorescent beads (each bead has a specific known color) coated with known HLA antigens are incubated with patient serum. If present, HLA-specific antibodies bind to the HLA antigens on the beads. The red laser excites the fluorochrome within the beads and classifies the bead, while the green laser detects the fluorescence signal of phycoerythrin conjugated to the secondary antibody. The combination of the two signals defines antibody specificity. Data are acquired, processed and analyzed by the Luminex platform and fluorescence intensity is expressed as mean fluorescence intensity (MFI).</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Julien Vionnet, Christine Sempoux, Manuel Pascual, Alberto Sánchez-Fueyo, Jordi Colmenero" "autores" => array:5 [ 0 => array:2 [ "nombre" => "Julien" "apellidos" => "Vionnet" ] 1 => array:2 [ "nombre" => "Christine" "apellidos" => "Sempoux" ] 2 => array:2 [ "nombre" => "Manuel" "apellidos" => "Pascual" ] 3 => array:2 [ "nombre" => "Alberto" "apellidos" => "Sánchez-Fueyo" ] 4 => array:2 [ "nombre" => "Jordi" "apellidos" => "Colmenero" ] ] ] ] ] "idiomaDefecto" => "en" "Traduccion" => array:1 [ "en" => array:9 [ "pii" => "S2444382419302482" "doi" => "10.1016/j.gastre.2019.09.004" "estado" => "S300" "subdocumento" => "" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2444382419302482?idApp=UINPBA00004N" ] ] "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0210570519302547?idApp=UINPBA00004N" "url" => "/02105705/0000004300000001/v1_202001221437/S0210570519302547/v1_202001221437/en/main.assets" ] "itemAnterior" => array:19 [ "pii" => "S0210570519301761" "issn" => "02105705" "doi" => "10.1016/j.gastrohep.2019.07.008" "estado" => "S300" "fechaPublicacion" => "2020-01-01" "aid" => "1432" "copyright" => "Elsevier España, S.L.U." "documento" => "article" "crossmark" => 1 "subdocumento" => "sco" "cita" => "Gastroenterol Hepatol. 2020;43:30-1" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:2 [ "total" => 110 "formatos" => array:2 [ "HTML" => 84 "PDF" => 26 ] ] "es" => array:11 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Imagen del mes</span>" "titulo" => "Divertículo gigante de sigma" "tienePdf" => "es" "tieneTextoCompleto" => "es" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "30" "paginaFinal" => "31" ] ] "titulosAlternativos" => array:1 [ "en" => array:1 [ "titulo" => "Giant sigmoid diverticulum" ] ] "contieneTextoCompleto" => array:1 [ "es" => true ] "contienePdf" => array:1 [ "es" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0010" "etiqueta" => "Figura 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 888 "Ancho" => 805 "Tamanyo" => 93771 ] ] "descripcion" => array:1 [ "es" => "<p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">RM coronal.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Mikel Fiallegas Cano, Irene Moraleja Yudego, Blanca Sampedro Andrada, Leticia de Castro Santos, Itxaso Jiménez Iragorri, Noelia Martín Vicente, Jose Luis Cabriada Nuno" "autores" => array:7 [ 0 => array:2 [ "nombre" => "Mikel" "apellidos" => "Fiallegas Cano" ] 1 => array:2 [ "nombre" => "Irene" "apellidos" => "Moraleja Yudego" ] 2 => array:2 [ "nombre" => "Blanca" "apellidos" => "Sampedro Andrada" ] 3 => array:2 [ "nombre" => "Leticia" "apellidos" => "de Castro Santos" ] 4 => array:2 [ "nombre" => "Itxaso" "apellidos" => "Jiménez Iragorri" ] 5 => array:2 [ "nombre" => "Noelia" "apellidos" => "Martín Vicente" ] 6 => array:2 [ "nombre" => "Jose Luis" "apellidos" => "Cabriada Nuno" ] ] ] ] ] "idiomaDefecto" => "es" "Traduccion" => array:1 [ "en" => array:9 [ "pii" => "S2444382419302524" "doi" => "10.1016/j.gastre.2019.07.007" "estado" => "S300" "subdocumento" => "" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2444382419302524?idApp=UINPBA00004N" ] ] "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0210570519301761?idApp=UINPBA00004N" "url" => "/02105705/0000004300000001/v1_202001221437/S0210570519301761/v1_202001221437/es/main.assets" ] "en" => array:13 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Image of the month</span>" "titulo" => "Late closure by secondary intention of a large gastrocutaneous fistula after complicated percutaneous endoscopic gastrostomy" "tieneTextoCompleto" => true "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "32" "paginaFinal" => "33" ] ] "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "Marisa Linhares, Flávio Pereira, Richard Azevedo, Rui Sousa, António Banhudo" "autores" => array:5 [ 0 => array:4 [ "nombre" => "Marisa" "apellidos" => "Linhares" "email" => array:1 [ 0 => "marisa.d.linhares@gmail.com" ] "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">*</span>" "identificador" => "cor0005" ] ] ] 1 => array:2 [ "nombre" => "Flávio" "apellidos" => "Pereira" ] 2 => array:2 [ "nombre" => "Richard" "apellidos" => "Azevedo" ] 3 => array:2 [ "nombre" => "Rui" "apellidos" => "Sousa" ] 4 => array:2 [ "nombre" => "António" "apellidos" => "Banhudo" ] ] "afiliaciones" => array:1 [ 0 => array:2 [ "entidad" => "Department of Gastroenterology, ULS Castelo Branco, Portugal" "identificador" => "aff0005" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Cicatrización tardía por segunda intención de una fístula enterocutánea de gran tamaño tras gastrostomía endoscópica percutánea complicada" ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 616 "Ancho" => 1250 "Tamanyo" => 67881 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Large gastrocutaneous fistula after PEG tube removal (A) and its closure after one month of conservative treatment (B).</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall">This is a case of 81 years-old male, with previous diagnosis of a laryngeal stage IV tumour with chronic use of nasogastric tube. He is referenced to be submitted to a percutaneous endoscopy gastrostomy.</p><p id="par0010" class="elsevierStylePara elsevierViewall">The procedure was successful with no immediate complications. Next day, the patient developed local and systemic inflammatory signs. Is observed a peristomal leakage and wound infection which are managed with conservative treatment. After four days there is no improvement and PEG tube is removed to facilitate the fistula closure.</p><p id="par0015" class="elsevierStylePara elsevierViewall">Unlike expected, the fistula did not closed in next hours. The gastrocutaneous fistula became large with a high output drainage (400<span class="elsevierStyleHsp" style=""></span>mL/24<span class="elsevierStyleHsp" style=""></span>h) that caused an important cutaneous irritation (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>A). The fistula orifice had an important necrosed surround area that unable an endoscopic and surgical treatment. At this point, the last option was to intensify the conservative treatment with piperacillin-tazobactam (4.5<span class="elsevierStyleHsp" style=""></span>g, every 8<span class="elsevierStyleHsp" style=""></span>h for 10 days), proton pump inhibitors bid and intensive dressing care with adhesive powder, and wait for treatment response. One week later, gastrocutaneous fistula finally shows improvement, and one month later it was practically closed (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>B). We can observe a late spontaneous closure of a large gastrocutaneous fistula by secondary intention.</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0005">Funding</span><p id="par0020" class="elsevierStylePara elsevierViewall">All authors declare no funding on this work.</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Conflict of interest</span><p id="par0025" class="elsevierStylePara elsevierViewall">All authors declare no conflict of interest.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:2 [ 0 => array:2 [ "identificador" => "sec0005" "titulo" => "Funding" ] 1 => array:2 [ "identificador" => "sec0010" "titulo" => "Conflict of interest" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "multimedia" => array:1 [ 0 => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 616 "Ancho" => 1250 "Tamanyo" => 67881 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Large gastrocutaneous fistula after PEG tube removal (A) and its closure after one month of conservative treatment (B).</p>" ] ] ] ] "idiomaDefecto" => "en" "url" => "/02105705/0000004300000001/v1_202001221437/S0210570519301645/v1_202001221437/en/main.assets" "Apartado" => array:4 [ "identificador" => "75365" "tipo" => "SECCION" "es" => array:2 [ "titulo" => "Imágenes del mes" "idiomaDefecto" => true ] "idiomaDefecto" => "es" ] "PDF" => "https://static.elsevier.es/multimedia/02105705/0000004300000001/v1_202001221437/S0210570519301645/v1_202001221437/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0210570519301645?idApp=UINPBA00004N" ]
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