Genomas virales fragmentados sugieren contaminación para aguas de consumo humano

Article information

Resumen

Objetivo

Determinar la presencia de rotavirus y norovirus en agua para consumo humano en una localidad de Bogotá.

Materiales y métodos

Se recolectaron ocho muestras de agua por semana en ocho lugares diferentes del predio en estudio. Para determinar la presencia del genoma viral, las muestras se ultrafiltraron y se hizo detección viral por medio de la técnica RT-PCR, amplificando un segmento del gen VP6 del rotavirus y un segmento del ORF2 del norovirus. Este estudio se llevó a cabo en una institución universitaria ubicada en una zona periurbana cercana a Bogotá, a 45km del acueducto que surte de agua al predio. La zona se encuentra rodeada por fincas con ganado lechero, centros comerciales y cuatro cementerios. El agua se recolectó en recipientes de polipropileno. En total, fueron 64 muestras de cinco litros de agua cada una.

Resultados

La presencia viral fue de 12,5%, encontrándose rotavirus en cuatro y norovirus en cuatro de las 64 muestras colectadas. Además de presentar la importancia de la presencia viral en agua para consumo humano, en este documento se discute el significado que tiene el encontrar solo segmentos virales y no la partícula viral completa e infecciosa.

Palabras clave:

agua potable

rotavirus

norovirus

Abstract

Objective

To determine the presence of rotavirus and norovirus in drinking water in a northern neighborhood in Bogotá, Colombia.

Materials and methods

Eight weekly samples of water were collected and ultrafiltered in order to detect the viral genome presence. The technique used for detection of genome segments was RT-PCR. VP6 gene from rotavirus and ORF2 segment from norovirus were selected in order to find the virus in the water. This study was performed in a higher education institution located in a periurban zone near Bogotá, 45km away from the aqueduct that services the area. The zone is surrounded by dairy cattle farms, malls and four cemeteries. Water was collected in clean polypropylene containers. There were a total of 64 samples of 5 liters of water each.

Results

Viral segments were found in 12.5% of the samples. We found rotavirus in four samples and norovirus in another 4. Besides discussing the importance of viral contamination in drinking water, we discussed the meaning of finding only viral segments and not complete and infectious viral particles.

Key words:

drinking water

rotavirus

norovirus

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