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Role of genes GSTM1, GSTT1, and MnSOD in the development of late-onset Alzheimer disease and their relationship with APOE*4
Papel de las variantes GSTM1, GSTT1 y MnSOD en el desarrollo de enfermedad de Alzheimer de aparición tardía y su relación con el alelo 4 de APOE
E. de Mendonça, E. Salazar Alcalá, M. Fernández-Mestre
Corresponding author
Laboratorio de Fisiopatología, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela
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    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Neurodegenerative diseases&#44; including Alzheimer disease &#40;AD&#41;&#44; display oxidative damage as a common feature&#44; but it is unclear whether this process is the cause or the effect of the disease&#46;<a class="elsevierStyleCrossRef" href="#bib0215"><span class="elsevierStyleSup">1</span></a> Oxidative damage may be caused by the peptide amyloid &#946; &#40;A&#946;&#41; and the paired helical filaments of tau protein that constitute the typical lesions in AD&#46; The A&#946; peptide has been observed to give rise to free radicals<a class="elsevierStyleCrossRef" href="#bib0220"><span class="elsevierStyleSup">2</span></a> and inhibit the cytochrome oxidase enzyme&#46; This contributes to oxidative stress&#46;<a class="elsevierStyleCrossRef" href="#bib0225"><span class="elsevierStyleSup">3</span></a> Observations have also shown that the paired helical filaments of tau protein may form advanced glycation end-products&#44; and this generates enough reactive oxygen species &#40;ROS&#41; to cause oxidative damage&#46;<a class="elsevierStyleCrossRef" href="#bib0230"><span class="elsevierStyleSup">4</span></a> Since the brain is a major consumer of oxygen with a high demand for energy and only limited antioxidant ability compared to other tissues&#44; it is very susceptible to oxidative damage&#46; Antioxidant defences therefore play a vital role as the means of eliminating free radicals&#46;<a class="elsevierStyleCrossRef" href="#bib0235"><span class="elsevierStyleSup">5</span></a> Patients with AD have displayed altered activity of glutathione S-transferase enzymes &#40;GSTs&#41; and manganese superoxide dismutase &#40;MnSOD&#41;&#46;<a class="elsevierStyleCrossRef" href="#bib0240"><span class="elsevierStyleSup">6</span></a> GSTs are a family of enzymes that are crucial to the protection of cells against toxic substances and oxidative stress&#46; They are encoded by some 16 genes&#44; further subdivided into 8 classes&#46;<a class="elsevierStyleCrossRef" href="#bib0245"><span class="elsevierStyleSup">7</span></a> The &#956; class comprises 5 different isoenzymes named GSTM1 through GSTM5&#46; The &#952; class includes only 2 isoenzymes&#44; GSTT1 and GSTT2&#46; The null genotypes of genes <span class="elsevierStyleItalic">GSTM1</span> and <span class="elsevierStyleItalic">GSTT1</span> are characterised by homozygous knockout of the gene&#44; resulting in absent enzyme activity &#40;overview by Cooper<a class="elsevierStyleCrossRef" href="#bib0250"><span class="elsevierStyleSup">8</span></a>&#41;&#46; Both the GSTT1 and GSTM1 enzymes are known for their ability to catalyse the detoxification of reactive oxygen and products of lipid peroxidation&#46;<a class="elsevierStyleCrossRef" href="#bib0255"><span class="elsevierStyleSup">9</span></a> Because of this&#44; inactivity of the GSTT1&#47;M1 enzymes has been linked to greater exposure to oxidative stress&#46;<a class="elsevierStyleCrossRef" href="#bib0260"><span class="elsevierStyleSup">10</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">The superoxide dismutase enzyme &#40;SOD&#41; is one of the main defences against the damage that can be caused by the free radical superoxide &#40;O<span class="elsevierStyleSup">2&#8722;</span>&#41;&#46; The enzyme catalyses the conversion of superoxide into molecular oxygen &#40;O<span class="elsevierStyleInf">2</span>&#41; and hydrogen peroxide &#40;H<span class="elsevierStyleInf">2</span>O<span class="elsevierStyleInf">2</span>&#41;&#59; these molecules will later be converted into water by the action of catalase or glutathione peroxidase enzymes&#46;<a class="elsevierStyleCrossRef" href="#bib0265"><span class="elsevierStyleSup">11</span></a> Superoxide dismutase exists in 3 isoforms&#58; cytosolic &#91;Cu&#47;ZnSOD&#93;&#44; extracellular &#91;EC-SOD&#93;&#44; and mitochondrial &#91;MnSOD&#93;&#46;<a class="elsevierStyleCrossRef" href="#bib0270"><span class="elsevierStyleSup">12</span></a> Since 90&#37; of all ROS originate in the mitochondria&#44; MnSOD is an antioxidant which plays a fundamental role in protecting cells against oxidative stress&#46; The activity of this mitochondrial enzyme defends cells from lipid peroxidation&#59; within the brain&#44; it protects the viability of the neuronal membrane&#46;<a class="elsevierStyleCrossRef" href="#bib0275"><span class="elsevierStyleSup">13</span></a> MnSOD enzyme is synthesised in the cytosol and then transported to the mitochondria&#46; MnSOD transport requires the participation of a sequence of 24 amino acids known as the mitochondrial targeting sequence &#40;MTS&#41;&#46; These amino acids form the amphiphilic helices required to transport the enzyme to the mitochondria&#46;<a class="elsevierStyleCrossRef" href="#bib0280"><span class="elsevierStyleSup">14</span></a> The gene that encodes MnSOD possesses a polymorphism by which thymine &#40;T&#41; is replaced by cytosine &#40;C&#41; at nucleotide 47&#44; resulting in an amino acid substitution &#40;Val&#8594;Ala&#41; at position &#8722;9 of the MTS and altering the enzyme structure&#46; These changes in the structure alter mitochondrial transport of MnSOD&#44; and therefore affect the cell&#39;s ability to defend itself from superoxide&#46;<a class="elsevierStyleCrossRef" href="#bib0280"><span class="elsevierStyleSup">14</span></a> Multiple studies have now demonstrated the role MnSOD plays in how neurons survive oxidative stress&#46;<a class="elsevierStyleCrossRefs" href="#bib0285"><span class="elsevierStyleSup">15&#44;16</span></a> Considering that GSTT1&#44; GSTM1&#44; and MnSOD enzymes contribute to cell defence against oxidative stress&#44; and the possibility that oxidative stress and AD pathogenesis are related&#44; we aim to study the role of polymorphisms of the genes <span class="elsevierStyleItalic">GSTT1&#44; GSTM1</span> and <span class="elsevierStyleItalic">MnSOD</span> in the development of AD and correlate any such associations with the presence of allele ¿4 of the <span class="elsevierStyleItalic">APOE</span> gene&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Materials and methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Patients</span><p id="par0015" class="elsevierStylePara elsevierViewall">The study was carried out in 79 patients &#40;mean age&#44; 70<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>10 years&#41;&#44; all of whom had been diagnosed with sporadic AD&#46; All patients had received treatment in the neurology department at Hospital Cl&#237;nico Universitario de Caracas &#40;Venezuela&#41; at some time between September 2004 and October 2006&#46; These patients were selected according to the clinical protocol implemented in the Luis Borges Neuropsychology Unit of the neurology department at Hospital Cl&#237;nico Universitario de Caracas&#46; The protocol was framed in accordance with the specifications of the American Psychiatric Association &#40;DSM-IV&#41; and the NINCDS-ADRDA Alzheimer&#39;s Criteria &#40;National Institute of Neurological Disorders&#44; Communicative Disorders&#44; and Stroke&#59; Alzheimer&#39;s Disease and Related Disorders Association&#41;&#46;</p><p id="par0020" class="elsevierStylePara elsevierViewall">The control group consisted of 100 healthy Venezuelan residents with a mean age of 71<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>10 years&#46; These subjects completed the Mini-Mental State Examination and laboratory and imaging studies&#46;</p><p id="par0025" class="elsevierStylePara elsevierViewall">All participants in the study gave their informed consent&#59; the consent of participants with AD was authorised by their legal guardians&#46; The consent procedure was approved by the bioethics committees at Instituto Venezolano de Investigaciones Cient&#237;ficas and Hospital Cl&#237;nico Universitario de Caracas&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Genomic DNA extraction</span><p id="par0030" class="elsevierStylePara elsevierViewall">Genomic DNA was extracted from leukocytes and lymphocytes in peripheral blood according to Bunce&#39;s method&#46;<a class="elsevierStyleCrossRef" href="#bib0295"><span class="elsevierStyleSup">17</span></a></p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Detection of <span class="elsevierStyleItalic">GSTT1</span>&#47;<span class="elsevierStyleItalic">GSTM1</span> genes</span><p id="par0035" class="elsevierStylePara elsevierViewall">A multiplex PCR protocol was used to determine presence or absence of the <span class="elsevierStyleItalic">GSTM1</span> and <span class="elsevierStyleItalic">GSTT1</span> genes according to a modified version of the method described by Lin et al&#46;<a class="elsevierStyleCrossRef" href="#bib0300"><span class="elsevierStyleSup">18</span></a> and the specific initiators used by Chen et al&#46;<a class="elsevierStyleCrossRef" href="#bib0305"><span class="elsevierStyleSup">19</span></a></p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Genotyping of the <span class="elsevierStyleItalic">MnSOD</span> gene</span><p id="par0040" class="elsevierStylePara elsevierViewall">The <span class="elsevierStyleItalic">Ala-9Val</span> polymorphism was studied using the PCR-RFLP method with the initiators and protocol described by Ambrosone et al&#46;<a class="elsevierStyleCrossRef" href="#bib0310"><span class="elsevierStyleSup">20</span></a></p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Genotyping of the <span class="elsevierStyleItalic">APOE</span> gene</span><p id="par0045" class="elsevierStylePara elsevierViewall">Researchers genotyped and evaluated the <span class="elsevierStyleItalic">APOE</span> polymorphism using PCR-RFLP with the initiators described by Emi et al&#46;<a class="elsevierStyleCrossRef" href="#bib0315"><span class="elsevierStyleSup">21</span></a> and the protocol published by Hixson and Vernier&#46;<a class="elsevierStyleCrossRef" href="#bib0320"><span class="elsevierStyleSup">22</span></a></p></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Statistical analysis</span><p id="par0050" class="elsevierStylePara elsevierViewall">Genotypic and allelic frequencies were calculated&#46; The statistical significance of intergroup differences in frequency &#40;for alleles&#44; genotypes&#44; and genotypic combinations&#41; was estimated using the Mantel&#8211;Haenszel chi-square statistic and 2<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>2 contingency tables&#46; Researchers adjusted <span class="elsevierStyleItalic">P</span>-values by multiplying them by the number of completed comparisons &#40;Bonferroni correction&#41;&#46; Differences were considered statistically significant for <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>&#46;05&#46;</p></span></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Results</span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0110">Genotype distribution and genotypic combination of <span class="elsevierStyleItalic">GSTT1</span> and <span class="elsevierStyleItalic">GSTM1</span> genes in healthy individuals and patients with Alzheimer disease</span><p id="par0055" class="elsevierStylePara elsevierViewall"><a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a> displays the distribution of the <span class="elsevierStyleItalic">GSTM1</span> &#40;wild-type &#91;&#43;&#93;&#44; null &#91;&#8722;&#93;&#41; and <span class="elsevierStyleItalic">GSTT1</span> &#40;wild-type &#91;&#43;&#93;&#44; null &#91;&#8722;&#93;&#41; in healthy individuals and patients with AD&#46; The comparison of frequencies revealed the wild-type <span class="elsevierStyleItalic">GSTM1</span> genotype to be significantly more frequent in controls than in patients &#40;OR&#58; 0&#46;58&#59; 95&#37; CI&#58; 0&#46;3235-1&#46;0659&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;04&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41;&#46; In contrast&#44; the null <span class="elsevierStyleItalic">GSTM1</span> genotype had a significantly higher frequency in patients than in controls &#40;OR&#58; 1&#46;7&#59; 95&#37; CI&#58; 0&#46;9381-3&#46;0903&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;04&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41;&#46; Nevertheless&#44; differences were not statistically significant once <span class="elsevierStyleItalic">P</span>-values were corrected&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0060" class="elsevierStylePara elsevierViewall">The study of genotype combinations for <span class="elsevierStyleItalic">GSTT1</span> and <span class="elsevierStyleItalic">GSTM1</span> found that all possible combinations were present in both groups&#46; The <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#8722;</span> combination was the most frequent in patients with AD&#44; followed by the combinations <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1</span>&#43;&#44; <span class="elsevierStyleItalic">GSTT1&#8722;&#47;GSTM1</span>&#43;&#44; and <span class="elsevierStyleItalic">GSTT1&#8722;&#47;GSTM1&#8722;</span>&#46; The <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#43;</span> combination was the most frequent among healthy controls&#44; followed by the combinations <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#8722;</span>&#44; <span class="elsevierStyleItalic">GSTT1&#8722;&#47;GSTM1</span>&#43;&#44; and <span class="elsevierStyleItalic">GSTT1&#8722;&#47;GSTM1&#8722;</span>&#46; The comparison of frequencies revealed the combination <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#8722;</span> to be significantly more frequent in patients than in controls &#40;OR&#58; 2&#46;06&#59; 95&#37; CI&#58; 1&#46;1118-3&#46;8036&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;09&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;036&#41;&#46; The <span class="elsevierStyleItalic">P</span>-value remained significant after correction &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46;</p></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0115">Allelic and genotypic frequencies of the <span class="elsevierStyleItalic">MnSOD</span> gene in healthy individuals and patients with Alzheimer disease</span><p id="par0065" class="elsevierStylePara elsevierViewall">In both groups&#44; the <span class="elsevierStyleItalic">Ala&#47;Val</span> genotype had the highest frequency&#44; followed by <span class="elsevierStyleItalic">Ala&#47;Ala</span> and <span class="elsevierStyleItalic">Val&#47;Val</span> in AD patients and <span class="elsevierStyleItalic">Val&#47;Val</span> and <span class="elsevierStyleItalic">Ala&#47;Ala</span> in healthy controls&#46; The comparison of frequencies revealed the <span class="elsevierStyleItalic">Ala&#47;Val</span> genotype to be significantly more frequent in patients than in controls &#40;OR&#58; 1&#46;9&#59; 95&#37; CI&#58; 0&#46;9147-3&#46;9498&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;04&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41;&#46; However&#44; differences were not statistically significant once <span class="elsevierStyleItalic">P</span>-values were corrected &#40;<a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#41;&#46;</p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0120">Allelic and genotypic frequency distributions of the <span class="elsevierStyleItalic">MnSOD</span> gene in healthy individuals and patients with Alzheimer disease</span><p id="par0070" class="elsevierStylePara elsevierViewall"><a class="elsevierStyleCrossRef" href="#tbl0015">Table 3</a> displays the genotypes of the <span class="elsevierStyleItalic">APOE</span> gene for AD patients and healthy controls&#46; The frequencies of genotypes <span class="elsevierStyleItalic">¿2¿3</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;04&#59; 95&#37; CI&#58; 0&#46;002-0&#46;764&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;0007&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;0035&#41; and <span class="elsevierStyleItalic">¿3¿3</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;55&#59; IC 95&#37;&#58; 0&#46;3073-1&#46;0136&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;02&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41; were significantly higher in controls than in AD patients&#46; In contrast&#44; patients displayed higher frequencies of genotypes <span class="elsevierStyleItalic">¿3¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>1&#46;93&#59; 95&#37; CI&#58; 1&#46;0164-3&#46;6980&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;02&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41; and <span class="elsevierStyleItalic">¿4¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>4&#46;29&#59; 95&#37; CI&#58; 1&#46;3241-13&#46;9016&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;004&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;02&#41;&#46; The comparison of allele frequencies also showed that <span class="elsevierStyleItalic">¿2</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;04&#59; 95&#37; CI&#58; 0&#46;0059-0&#46;3651&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;000025&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;000075&#41; and allele <span class="elsevierStyleItalic">¿3</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;5&#59; 95&#37; CI&#58; 0&#46;3220-0&#46;8195&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;0024&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;0072&#41; were significantly more common among controls than in AD patients&#46; Nevertheless&#44; allele <span class="elsevierStyleItalic">¿4</span> was significantly more frequent in AD patients than in controls &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>2&#46;8&#59; 95&#37; CI&#58; 1&#46;7003-4&#46;6221&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;000019&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;000057&#41;&#46;</p><elsevierMultimedia ident="tbl0015"></elsevierMultimedia></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0125">Study of the combined effect of polymorphism in <span class="elsevierStyleItalic">GSTT1</span>&#44; <span class="elsevierStyleItalic">GSTM1</span> and <span class="elsevierStyleItalic">APOE</span> genes</span><p id="par0075" class="elsevierStylePara elsevierViewall">There are 24 possible combinations of <span class="elsevierStyleItalic">GSTT1&#47;GSTM1&#47;APOE</span>&#44; and we observed 11 in the AD group and 15 in the controls&#46; The frequencies of the combinations <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#43;&#47;¿2¿3</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;1&#59; 95&#37; CI&#58; 0&#46;0059-2&#46;004&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;02&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41; and <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#43;&#47;¿3¿3</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;46&#59; 95&#37; CI&#58; 0&#46;2114-1&#46;002&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;02&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41; were significantly higher in controls than in patients&#46; In contrast&#44; frequencies of the combinations <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#8722;&#47;¿3¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3&#46;07&#59; 95&#37; CI&#58; 1&#46;0210-9&#46;2514&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;019&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41; and <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#8722;&#47;¿4¿</span>4 &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>5&#46;52&#59; 95&#37; CI&#58; 1&#46;1381-26&#46;7837&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;009&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41; were significantly higher in AD patients than in controls &#40;<a class="elsevierStyleCrossRef" href="#tbl0020">Table 4</a>&#41;&#46;</p><elsevierMultimedia ident="tbl0020"></elsevierMultimedia></span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0130">Study of the combined effect of the polymorphism <span class="elsevierStyleItalic">Ala-9Val</span> in the genes <span class="elsevierStyleItalic">MnSOD</span> and <span class="elsevierStyleItalic">APOE</span></span><p id="par0080" class="elsevierStylePara elsevierViewall">Of the 18 possible combinations of <span class="elsevierStyleItalic">MnSOD&#47;APOE</span>&#44; 12 were observed in controls and 9 in patients&#46; The combinations <span class="elsevierStyleItalic">AlaAla&#47;¿3¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3&#46;4&#59; 95&#37; CI&#58; 1&#46;0476-11&#46;5486&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;016&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41; and <span class="elsevierStyleItalic">AlaVal&#47;¿4¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>7&#46;9&#59; 95&#37; CI&#58; 1&#46;7027-36&#46;8988&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;001&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41; were significantly more common in AD patients than in controls&#46; We must point out that some genotype combinations were present only in the control group&#58; <span class="elsevierStyleItalic">AlaVal&#47;¿2¿3</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;09&#59; 95&#37; CI&#58; 0&#46;0050-1&#46;6480&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;014&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41;&#44; <span class="elsevierStyleItalic">AlaVal&#47;¿3¿3</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;5&#59; 95&#37; CI&#58; 0&#46;2642-0&#46;9461&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;016&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41;&#44; and <span class="elsevierStyleItalic">ValVal&#47;¿2¿3</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;1&#59; 95&#37; CI&#58; 0&#46;0059-2&#46;0049&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;02&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41; &#40;<a class="elsevierStyleCrossRef" href="#tbl0025">Table 5</a>&#41;&#46;</p><elsevierMultimedia ident="tbl0025"></elsevierMultimedia></span></span><span id="sec0075" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0135">Discussion</span><p id="par0085" class="elsevierStylePara elsevierViewall">The imbalance between the production and the elimination of ROS and nitrogen is known as oxidative stress&#46; The cell must overcome oxidative stress to restore redox balance and thereby avoid the neuronal death and loss of neuronal function that are associated with neurodegenerative diseases&#46;<a class="elsevierStyleCrossRef" href="#bib0325"><span class="elsevierStyleSup">23</span></a> Studies in the area of AD pathogenesis have focused on the role of oxidative stress since the central nervous system is a great consumer of energy with only minimal antioxidant defences&#44; making it highly sensitive to oxidative stress&#46;<a class="elsevierStyleCrossRef" href="#bib0330"><span class="elsevierStyleSup">24</span></a> In the brain with AD&#44; antioxidant systems function less effectively&#44; which may lead to an increase in the reactive species of oxygen and nitrogen that would react to biomolecules&#44; including proteins&#44; lipids&#44; carbohydrates&#44; DNA&#44; and RNA&#46; This would cause changes in their chemical structure&#44; and in turn&#44; loss of function&#46;<a class="elsevierStyleCrossRef" href="#bib0335"><span class="elsevierStyleSup">25</span></a> Oxidative stress in the brains of AD patients is well-documented&#58; researchers have observed high levels of antioxidant enzymes&#44; as well as increased concentrations of oxidative stress markers in the brains of AD patients compared to those of age-matched controls&#46; Furthermore&#44; the role played by oxidative stress in AD progression was observed when we compared the presence of the same brain proteins with oxidative damage in subjects with mild cognitive impairment&#44; early-stage AD&#44; and late-stage AD&#46; This demonstrates that certain major pathways are activated and may be involved in AD progression&#46;<a class="elsevierStyleCrossRef" href="#bib0335"><span class="elsevierStyleSup">25</span></a> Given that the GST family of enzymes conjugates reduced glutathione with electrophilic compounds&#44; thus helping remove it from cells to prevent oxidative damage&#44;<a class="elsevierStyleCrossRef" href="#bib0340"><span class="elsevierStyleSup">26</span></a> several studies have been published on the genes that encode the GSTM1 and GSTT1 enzymes and their role in different diseases&#46; These enzymes proceed from a null phenotype characterised by absence of enzymatic activity due to inherited homozygous elimination of the full gene&#46; In this study&#44; the null <span class="elsevierStyleItalic">GSTM1</span> genotype was significantly more frequent in patients with AD &#40;OR&#58; 1&#46;7&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;04&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41;&#59; the wild-type <span class="elsevierStyleItalic">GSTM1</span> genotype was significantly more common in healthy controls &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;58&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;04&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>ns&#41;&#44; in contrast with findings from other studies&#46;<a class="elsevierStyleCrossRefs" href="#bib0255"><span class="elsevierStyleSup">9&#44;27&#8211;30</span></a> Likewise&#44; the combination <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#8722;</span> was more frequently seen in AD patients than in controls &#40;OR&#58; 2&#46;06&#59; <span class="elsevierStyleItalic">P</span>corr<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;036&#41;&#46; These results suggest that the null genotype <span class="elsevierStyleItalic">GSTM1</span> and the combination <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#8722;</span> could confer up to twice the risk of developing AD&#46; Considering that a variety of studies suggest that variations in the <span class="elsevierStyleItalic">APOE</span> gene may be genetic risk factors for AD&#44;<a class="elsevierStyleCrossRefs" href="#bib0365"><span class="elsevierStyleSup">31&#8211;33</span></a> we recorded the combinations of <span class="elsevierStyleItalic">GSTT1&#47;GSTM1</span> and <span class="elsevierStyleItalic">APOE</span> genotypes and obtained interesting results&#46; The genotype combinations <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#8722;&#47;¿3¿4</span> and <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#8722;&#47;¿4¿4</span> were significantly more frequent in patients than in controls&#46; These combinations may therefore be linked to increased likelihood of developing AD&#46; Additionally&#44; the risk posed by the presence of one or 2 <span class="elsevierStyleItalic">¿4</span> alleles of the <span class="elsevierStyleItalic">APOE</span> gene is higher in the combination in which the <span class="elsevierStyleItalic">GSTM1</span> gene is absent&#46; Considering the above&#44; an increase in A&#946; peptide aggregation due to the E4 isoform of apolipoprotein E&#44;<a class="elsevierStyleCrossRef" href="#bib0380"><span class="elsevierStyleSup">34</span></a> together with the reduction in antioxidant defences caused by homozygotic elimination of the <span class="elsevierStyleItalic">GSTM1</span> gene&#44; could generate greater oxidative stress&#46; This in turn would give rise to more neuronal death and explain how the risk inherent to allele ¿4 of the <span class="elsevierStyleItalic">APOE</span> gene would multiply in the absence of the <span class="elsevierStyleItalic">GSTM1</span> gene&#46; Furthermore&#44; combinations including wild-type <span class="elsevierStyleItalic">GSTT1</span> and <span class="elsevierStyleItalic">GSTM1</span> and at least one <span class="elsevierStyleItalic">¿3</span> allele &#91;<span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#43;&#47;¿2¿3</span> and <span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#43;&#47;¿3¿3</span>&#93; were significantly more frequent in healthy controls than in AD patients&#46; This suggests that these combinations may provide protection against the onset of AD&#46; A&#946; peptides are components of the senile plaques that begin degeneration of brain neurons in AD by increasing the presence of ROS until it exceeds the defence ability of each cell&#46; Kaminsky and Kosenko<a class="elsevierStyleCrossRef" href="#bib0385"><span class="elsevierStyleSup">35</span></a> studied the effects of A&#946; peptides on antioxidant enzymes from mitochondrial and non-mitochondrial sources in the rat brain in vivo&#46; They observed that A&#946; peptides increase the enzymatic activities creating H<span class="elsevierStyleInf">2</span>O<span class="elsevierStyleInf">2</span> while inhibiting the activity of enzymes that consume H<span class="elsevierStyleInf">2</span>O<span class="elsevierStyleInf">2</span> in the mitochondria and cytosol&#46; This finding suggests that the imbalance between enzymes generating and those metabolising H<span class="elsevierStyleInf">2</span>O<span class="elsevierStyleInf">2</span> contribute to the underlying oxidative stress in the neurodegeneration and neuronal death that occur in AD&#46; Furthermore&#44; the A&#946; peptide has been found to be involved in the decreased expression of cytochrome c oxidase in the mitochondria&#44; which in turn affects the electron transport chain and produces ROS&#46;<a class="elsevierStyleCrossRef" href="#bib0225"><span class="elsevierStyleSup">3</span></a> The MnSOD enzyme forms part of the cell&#39;s enzymatic antioxidant defences&#44; and in fact provides the cell&#39;s first line of defence against the superoxide anion by converting it into H<span class="elsevierStyleInf">2</span>O<span class="elsevierStyleInf">2</span>&#46;<a class="elsevierStyleCrossRef" href="#bib0270"><span class="elsevierStyleSup">12</span></a> Multiple studies now support that MnSOD plays a role in how neurons survive oxidative stress&#46; One such study&#44; performed in <span class="elsevierStyleItalic">MnSOD</span> gene-knockout mice&#44; reported that the mice died not long after birth with signs of neurodegeneration&#46;<a class="elsevierStyleCrossRefs" href="#bib0285"><span class="elsevierStyleSup">15&#44;16</span></a> Similarly&#44; studies in transgenic rats have reported that MnSOD deficiency increases the concentrations of the A&#946; peptide&#44; which favours the formation of senile plaques&#46;<a class="elsevierStyleCrossRef" href="#bib0390"><span class="elsevierStyleSup">36</span></a> However&#44; other studies have reported that overexpression of the MnSOD enzyme actually protects neurons from oxidative damage&#46;<a class="elsevierStyleCrossRefs" href="#bib0395"><span class="elsevierStyleSup">37&#8211;39</span></a> In light of this conflicting evidence&#44; we studied the <span class="elsevierStyleItalic">Ala-9Val</span> polymorphism of the <span class="elsevierStyleItalic">MnSOD</span> gene that alters the secondary structure of that protein&#44; and therefore the mitochondrial transport of MnSOD&#46; This affects the cell&#39;s ability to defend itself from superoxide radicals&#46;<a class="elsevierStyleCrossRef" href="#bib0410"><span class="elsevierStyleSup">40</span></a> A comparison of the frequencies of the <span class="elsevierStyleItalic">Ala-9Val</span> genotypes of <span class="elsevierStyleItalic">MnSOD</span> showed that genotype <span class="elsevierStyleItalic">Ala&#47;Ala</span> was significantly more frequent in patients than in healthy controls&#46; This finding does not coincide with the results from an Italian population reported by Ventriglia et al&#46;<a class="elsevierStyleCrossRef" href="#bib0275"><span class="elsevierStyleSup">13</span></a> Furthermore&#44; evaluating the combined effect of the <span class="elsevierStyleItalic">MnSOD</span> and <span class="elsevierStyleItalic">APOE</span> genes revealed that the frequencies of the combinations <span class="elsevierStyleItalic">AlaAla&#47;¿3¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3&#46;4&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;03&#41; and <span class="elsevierStyleItalic">AlaVal&#47;¿4¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>7&#46;9&#59; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;01&#41; were significantly higher in patients than in controls&#44; meaning that these combinations may indicate a greater susceptibility to AD&#46; The presence of the <span class="elsevierStyleItalic">Ala</span> allele&#44; which is associated with greater enzymatic activity of MnSOD in humans&#44; as well as the presence of allele ¿<span class="elsevierStyleItalic">4</span>&#44; promotes the development of AD&#46; We should point out that the risk associated with genotypes ¿<span class="elsevierStyleItalic">3</span>¿<span class="elsevierStyleItalic">4</span> and ¿<span class="elsevierStyleItalic">4</span>¿<span class="elsevierStyleItalic">4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>1&#46;93 and OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>4&#46;29&#44; respectively&#41; is approximately twice as high when the genotypes <span class="elsevierStyleItalic">AlaAla</span> and <span class="elsevierStyleItalic">AlaVal</span> of the <span class="elsevierStyleItalic">MnSOD</span> gene are present &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3&#46;4 and OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>7&#46;9&#44; respectively&#41;&#46; In contrast&#44; the genotypic combinations <span class="elsevierStyleItalic">AlaVal&#47;¿2¿3</span>&#44; <span class="elsevierStyleItalic">AlaVal&#47;¿3¿3</span>&#44; and <span class="elsevierStyleItalic">ValVal&#47;¿2¿3</span> were only present in healthy individuals&#46; This suggests that they may play a protective role against AD&#46; Allele ¿<span class="elsevierStyleItalic">3</span>&#44; whether present homozygously or with allele ¿<span class="elsevierStyleItalic">2</span>&#44; together with one or 2 doses of the <span class="elsevierStyleItalic">Val</span> allele that is associated with abnormal enzymatic activity by MnSOD&#44;<a class="elsevierStyleCrossRefs" href="#bib0410"><span class="elsevierStyleSup">40&#44;41</span></a> would therefore protect individuals from developing AD&#46; Considering that the <span class="elsevierStyleItalic">Ala</span> form of MnSOD is more efficiently transported to the mitochondria than the <span class="elsevierStyleItalic">Val</span> form&#44;<a class="elsevierStyleCrossRef" href="#bib0280"><span class="elsevierStyleSup">14</span></a> and that this enzyme catalyses the dismutation of the superoxide anion in H<span class="elsevierStyleInf">2</span>O<span class="elsevierStyleInf">2</span> so that it can subsequently be removed from the mitochondria by the action of catalase and glutathione peroxidase&#44; researchers have suggested that the increased enzymatic activity of MnSOD would result in more production of H<span class="elsevierStyleInf">2</span>O<span class="elsevierStyleInf">2</span>&#46; This in turn would create an imbalance between H<span class="elsevierStyleInf">2</span>O<span class="elsevierStyleInf">2</span> generation and metabolism that would promote oxidative stress and damage within the cell&#46; Such an imbalance might be due to reduced activity of the enzymes contributing to H<span class="elsevierStyleInf">2</span>O<span class="elsevierStyleInf">2</span> elimination&#44; such as catalase and glutathione peroxidase&#46;<a class="elsevierStyleCrossRefs" href="#bib0385"><span class="elsevierStyleSup">35&#44;42</span></a> The results support the hypothesis that deterioration of the mitochondrial function and increased oxidative damage are involved in AD pathogenesis&#46; It is important&#44; nonetheless&#44; to point out that other genes linked to oxidative stress and antioxidant pathways may also affect susceptibility to developing AD&#44; and that their genetic variability should therefore be studied&#46;</p></span><span id="sec0080" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0140">Conflict of interest</span><p id="par0090" class="elsevierStylePara elsevierViewall">The authors have no conflict of interest to declare&#46;</p></span></span>"
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          "identificador" => "xres735095"
          "titulo" => "Abstract"
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            0 => array:2 [
              "identificador" => "abst0005"
              "titulo" => "Introduction"
            ]
            1 => array:2 [
              "identificador" => "abst0010"
              "titulo" => "Methods"
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            2 => array:2 [
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              "titulo" => "Results"
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              "titulo" => "Conclusions"
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          "identificador" => "xpalclavsec738832"
          "titulo" => "Keywords"
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        2 => array:3 [
          "identificador" => "xres735096"
          "titulo" => "Resumen"
          "secciones" => array:4 [
            0 => array:2 [
              "identificador" => "abst0025"
              "titulo" => "Introducci&#243;n"
            ]
            1 => array:2 [
              "identificador" => "abst0030"
              "titulo" => "M&#233;todos"
            ]
            2 => array:2 [
              "identificador" => "abst0035"
              "titulo" => "Resultados"
            ]
            3 => array:2 [
              "identificador" => "abst0040"
              "titulo" => "Conclusiones"
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        3 => array:2 [
          "identificador" => "xpalclavsec738831"
          "titulo" => "Palabras clave"
        ]
        4 => array:2 [
          "identificador" => "sec0005"
          "titulo" => "Introduction"
        ]
        5 => array:3 [
          "identificador" => "sec0010"
          "titulo" => "Materials and methods"
          "secciones" => array:6 [
            0 => array:2 [
              "identificador" => "sec0015"
              "titulo" => "Patients"
            ]
            1 => array:2 [
              "identificador" => "sec0020"
              "titulo" => "Genomic DNA extraction"
            ]
            2 => array:2 [
              "identificador" => "sec0025"
              "titulo" => "Detection of GSTT1&#47;GSTM1 genes"
            ]
            3 => array:2 [
              "identificador" => "sec0030"
              "titulo" => "Genotyping of the MnSOD gene"
            ]
            4 => array:2 [
              "identificador" => "sec0035"
              "titulo" => "Genotyping of the APOE gene"
            ]
            5 => array:2 [
              "identificador" => "sec0040"
              "titulo" => "Statistical analysis"
            ]
          ]
        ]
        6 => array:3 [
          "identificador" => "sec0045"
          "titulo" => "Results"
          "secciones" => array:5 [
            0 => array:2 [
              "identificador" => "sec0050"
              "titulo" => "Genotype distribution and genotypic combination of GSTT1 and GSTM1 genes in healthy individuals and patients with Alzheimer disease"
            ]
            1 => array:2 [
              "identificador" => "sec0055"
              "titulo" => "Allelic and genotypic frequencies of the MnSOD gene in healthy individuals and patients with Alzheimer disease"
            ]
            2 => array:2 [
              "identificador" => "sec0060"
              "titulo" => "Allelic and genotypic frequency distributions of the MnSOD gene in healthy individuals and patients with Alzheimer disease"
            ]
            3 => array:2 [
              "identificador" => "sec0065"
              "titulo" => "Study of the combined effect of polymorphism in GSTT1&#44; GSTM1 and APOE genes"
            ]
            4 => array:2 [
              "identificador" => "sec0070"
              "titulo" => "Study of the combined effect of the polymorphism Ala-9Val in the genes MnSOD and APOE"
            ]
          ]
        ]
        7 => array:2 [
          "identificador" => "sec0075"
          "titulo" => "Discussion"
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        8 => array:2 [
          "identificador" => "sec0080"
          "titulo" => "Conflict of interest"
        ]
        9 => array:1 [
          "titulo" => "References"
        ]
      ]
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    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2014-05-03"
    "fechaAceptado" => "2014-10-10"
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        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Keywords"
          "identificador" => "xpalclavsec738832"
          "palabras" => array:6 [
            0 => "Polymorphism"
            1 => "Alzheimer disease"
            2 => "<span class="elsevierStyleItalic">GSTM1</span>"
            3 => "<span class="elsevierStyleItalic">GSTT1</span>"
            4 => "<span class="elsevierStyleItalic">MnSOD</span>"
            5 => "Oxidative damage"
          ]
        ]
      ]
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        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palabras clave"
          "identificador" => "xpalclavsec738831"
          "palabras" => array:6 [
            0 => "Polimorfismo"
            1 => "Alzheimer"
            2 => "<span class="elsevierStyleItalic">GSTM1</span>"
            3 => "<span class="elsevierStyleItalic">GSTT1</span>"
            4 => "<span class="elsevierStyleItalic">MnSOD</span>"
            5 => "Da&#241;o oxidativo"
          ]
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    "resumen" => array:2 [
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        "titulo" => "Abstract"
        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Introduction</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Several studies have reported increased oxidation of lipids&#44; proteins and DNA in the brains of patients with Alzheimer disease &#40;AD&#41;&#46; Moreover&#44; these patients display differences in the activity and polymorphisms of the genes encoding the enzymes GST &#40;T1&#44; M1&#41; and MnSOD&#46; For these reasons&#44; we designed a study of the variability in <span class="elsevierStyleItalic">GSTT1</span>&#44; <span class="elsevierStyleItalic">GSTM1</span>&#44; and <span class="elsevierStyleItalic">MnSOD</span> genes in healthy and AD groups from a Venezuelan population&#46;</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">We included 179 unrelated Venezuelan subjects classified as either AD patients &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>79&#41; or healthy individuals &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>100&#41;&#46; Presence or absence of the <span class="elsevierStyleItalic">GSTT1</span>&#47;<span class="elsevierStyleItalic">GSTM1</span> genes was determined using PCR-SSP&#44; and polymorphisms of <span class="elsevierStyleItalic">MnSOD</span> and <span class="elsevierStyleItalic">APOE</span> genes were identified with PCR-RFLP&#46;</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">The genotype <span class="elsevierStyleItalic">GSTT1</span>&#43;&#47;<span class="elsevierStyleItalic">GSTM1</span>&#8722; seems to favour development of AD &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>2&#46;06&#44; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;01&#41;&#46; The risk level is higher when it is combined with the ¿4 allele of the <span class="elsevierStyleItalic">APOE</span> gene&#58; <span class="elsevierStyleItalic">GSTT1</span>&#43;&#47;<span class="elsevierStyleItalic">GSTM1</span>&#8722;&#47;<span class="elsevierStyleItalic">¿3¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3&#46;07&#44; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;05&#41;&#44; <span class="elsevierStyleItalic">GSTT1</span>&#43;&#47;<span class="elsevierStyleItalic">GSTM1</span>&#8722;&#47;<span class="elsevierStyleItalic">¿4¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>5&#46;52&#44; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;02&#41;&#46; The <span class="elsevierStyleItalic">Ala-9Val</span> polymorphism does not appear to be related to AD&#46; However&#44; the presence of the Ala&#47;Ala genotype increases the risk provided by the ¿4 allele of the <span class="elsevierStyleItalic">APOE</span> gene&#58; <span class="elsevierStyleItalic">AlaAla&#47;¿3¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3&#46;47&#44; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;03&#41;&#44; <span class="elsevierStyleItalic">AlaAla&#47;¿4¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>6&#46;3&#44; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>&#46;01&#41;&#46;</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">The results support the hypothesis that impaired mitochondrial function and increased oxidative damage are involved in the pathogenesis of AD&#46; It is important to study other genes related to oxidative stress and antioxidant pathways which could be involved in susceptibility to AD&#46;</p></span>"
        "secciones" => array:4 [
          0 => array:2 [
            "identificador" => "abst0005"
            "titulo" => "Introduction"
          ]
          1 => array:2 [
            "identificador" => "abst0010"
            "titulo" => "Methods"
          ]
          2 => array:2 [
            "identificador" => "abst0015"
            "titulo" => "Results"
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          3 => array:2 [
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            "titulo" => "Conclusions"
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      "es" => array:3 [
        "titulo" => "Resumen"
        "resumen" => "<span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Introducci&#243;n</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">Diversos estudios han descrito que en los cerebros de pacientes con enfermedad de Alzheimer &#40;EA&#41; hay una mayor oxidaci&#243;n de l&#237;pidos&#44; prote&#237;nas y ADN&#46; Adem&#225;s&#44; en estos pacientes se ha observado diferencias en la actividad y polimorfismos de los genes que codifican las enzimas GST &#40;T1 y M1&#41; y MnSOD&#46; En virtud de ello se plante&#243; estudiar la variabilidad de los genes <span class="elsevierStyleItalic">GSTT1</span>&#44; <span class="elsevierStyleItalic">GSTM1</span> y <span class="elsevierStyleItalic">MnSOD</span> en individuos venezolanos sanos y con EA&#46;</p></span> <span id="abst0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">M&#233;todos</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Se incluyeron 179 individuos venezolanos&#44; no relacionados&#44; agrupados en pacientes con EA &#40;n<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>79&#41; e individuos sanos &#40;n<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>100&#41;&#46; La presencia o ausencia de los genes <span class="elsevierStyleItalic">GSTT1</span>&#47;<span class="elsevierStyleItalic">GSTM1</span> se determin&#243; por PCR-SSP y los polimorfismo de los genes <span class="elsevierStyleItalic">MnSOD</span> y <span class="elsevierStyleItalic">APOE</span> por PCR-RFLP&#46;</p></span> <span id="abst0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Resultados</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">El genotipo <span class="elsevierStyleItalic">GSTT1</span>&#43;&#47;<span class="elsevierStyleItalic">GSTM1</span>&#8722; parece favorecer el desarrollo de la EA &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>2&#44;06&#59; p<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#44;01&#41;&#44; siendo el riesgo mayor al estar en combinaci&#243;n con el alelo ¿4 del gen <span class="elsevierStyleItalic">APOE</span>&#58; <span class="elsevierStyleItalic">GSTT1</span>&#43;&#47;<span class="elsevierStyleItalic">GSTM1</span>&#8722;&#47;<span class="elsevierStyleItalic">¿3¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3&#44;07&#59; p<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#44;05&#41;&#44; <span class="elsevierStyleItalic">GSTT1</span>&#43;&#47;<span class="elsevierStyleItalic">GSTM1</span>&#8722;&#47;<span class="elsevierStyleItalic">¿4¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>5&#44;52&#59; p<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#44;02&#41;&#46; El polimorfismo <span class="elsevierStyleItalic">Ala-9Val</span> por s&#237; solo no parece estar relacionado con la EA&#44; sin embargo&#44; la presencia del genotipo <span class="elsevierStyleItalic">Ala&#47;Ala</span> incrementa el riesgo que proporciona el alelo <span class="elsevierStyleItalic">¿4</span> del gen <span class="elsevierStyleItalic">APOE</span>&#58; <span class="elsevierStyleItalic">AlaAla&#47;¿3¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3&#44;47&#59; p<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#44;03&#41;&#44; <span class="elsevierStyleItalic">AlaAla&#47;¿4¿4</span> &#40;OR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>6&#44;3&#59; p<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#44;01&#41;&#46;</p></span> <span id="abst0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Conclusiones</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Los resultados apoyan la hip&#243;tesis de que el deterioro de la funci&#243;n mitocondrial y el aumento de da&#241;o oxidativo est&#225;n involucrados en la patog&#233;nesis de la EA&#46; Es importante estudiar otros genes relacionados con estr&#233;s oxidativo y v&#237;as antioxidantes&#44; los cuales pudiesen estar involucrados en la susceptibilidad a desarrollar la EA&#46;</p></span>"
        "secciones" => array:4 [
          0 => array:2 [
            "identificador" => "abst0025"
            "titulo" => "Introducci&#243;n"
          ]
          1 => array:2 [
            "identificador" => "abst0030"
            "titulo" => "M&#233;todos"
          ]
          2 => array:2 [
            "identificador" => "abst0035"
            "titulo" => "Resultados"
          ]
          3 => array:2 [
            "identificador" => "abst0040"
            "titulo" => "Conclusiones"
          ]
        ]
      ]
    ]
    "NotaPie" => array:1 [
      0 => array:2 [
        "etiqueta" => "&#9734;"
        "nota" => "<p class="elsevierStyleNotepara" id="npar0005">Please cite this article as&#58; de Mendon&#231;a E&#44; Salazar Alcal&#225; E&#44; Fern&#225;ndez-Mestre M&#46; Papel de las variantes <span class="elsevierStyleItalic">GSTM1</span>&#44; <span class="elsevierStyleItalic">GSTT1</span> y <span class="elsevierStyleItalic">MnSOD</span> en el desarrollo de enfermedad de Alzheimer de aparici&#243;n tard&#237;a y su relaci&#243;n con el alelo 4 de <span class="elsevierStyleItalic">APOE</span>&#46; Neurolog&#237;a&#46; 2016&#59;31&#58;535&#8211;542&#46;</p>"
      ]
    ]
    "multimedia" => array:5 [
      0 => array:8 [
        "identificador" => "tbl0005"
        "etiqueta" => "Table 1"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "detalles" => array:1 [
          0 => array:3 [
            "identificador" => "at1"
            "detalle" => "Table "
            "rol" => "short"
          ]
        ]
        "tabla" => array:2 [
          "leyenda" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">AD&#58; Alzheimer disease&#59; 95&#37; CI&#58; confidence interval&#59; ns&#58; not significant&#59; OR&#58; odds ratio&#46;</p><p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">Values shown in parentheses indicate the number of individuals bearing the genotype for the polymorphic site under study&#46; Frequencies are expressed as percentages&#46;</p><p id="spar1045" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleSup">&#42;</span> Significant after applying the Bonferroni correction&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">AD<br><span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>79&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Healthy<br><span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>100&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">OR<br>95&#37; CI&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">P</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " colspan="5" align="left" valign="top"><span class="elsevierStyleItalic">GSTT1 alleles</span></td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Wild-type &#40;&#43;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">83&#46;5 &#40;66&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">78 &#40;78&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">1&#46;43&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">ns&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Null &#40;&#8722;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">16&#46;5 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">22 &#40;22&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">0&#46;69&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">ns&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="5" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="5" align="left" valign="top"><span class="elsevierStyleItalic">GSTM1 alleles</span></td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Wild-type &#40;&#43;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">46&#46;8 &#40;37&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">60 &#40;60&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">0&#46;58&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;04&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Null &#40;&#8722;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">53&#46;2 &#40;42&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">40 &#40;40&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">1&#46;7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;04&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="5" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="5" align="left" valign="top"><span class="elsevierStyleItalic">Allele combinations</span></td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">GSTT1&#43; GSTM1&#8722;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">35&#46;4 &#40;28&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">47 &#40;47&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">0&#46;61&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;08&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">GSTT1&#8722; GSTM1&#8722;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">5&#46;1 &#40;4&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">9 &#40;9&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">0&#46;53&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">ns&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">GSTT1&#43; GSTM1&#8722;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">48&#46;1 &#40;38&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">31 &#40;31&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">2&#46;06&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;009<span class="elsevierStyleSup">&#42;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">GSTT1&#8722; GSTM1&#43;</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">11&#46;4 &#40;9&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">13 &#40;13&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">0&#46;86&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">ns&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab1213264.png"
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Distribution of genotypes and genotype combinations for the genes <span class="elsevierStyleItalic">GSTT1</span> and <span class="elsevierStyleItalic">GSTM1</span> in patients with AD and healthy controls&#46;</p>"
        ]
      ]
      1 => array:8 [
        "identificador" => "tbl0010"
        "etiqueta" => "Table 2"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "detalles" => array:1 [
          0 => array:3 [
            "identificador" => "at2"
            "detalle" => "Table "
            "rol" => "short"
          ]
        ]
        "tabla" => array:2 [
          "leyenda" => "<p id="spar0065" class="elsevierStyleSimplePara elsevierViewall">AD&#58; Alzheimer disease&#59; 95&#37; CI&#58; confidence interval&#59; ns&#58; not significant&#59; OR&#58; odds ratio&#46;</p><p id="spar0070" class="elsevierStyleSimplePara elsevierViewall">Values shown in parentheses indicate the number of repeats of the allele or the number of individuals bearing the genotype for the polymorphic site under study&#46; Frequencies are expressed as percentages&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">AD<br><span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>79&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Healthy<br><span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>100&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">OR<br>95&#37; CI&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">P</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " colspan="5" align="left" valign="top"><span class="elsevierStyleItalic">Genotypes</span></td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Ala&#47;Ala</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">26&#46;6 &#40;21&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">16 &#40;16&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">1&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;04&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Ala&#47;Val</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">54&#46;4 &#40;43&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">63 &#40;63&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">0&#46;7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">ns&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Val&#47;Val</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">19 &#40;15&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">21 &#40;21&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">0&#46;88&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">ns&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="5" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="5" align="left" valign="top"><span class="elsevierStyleItalic">Alleles</span></td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Ala</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">53&#46;8 &#40;85&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">47&#46;5 &#40;95&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">1&#46;28&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">ns&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Val</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">46&#46;2 &#40;73&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">52&#46;5 &#40;105&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">0&#46;77&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">ns&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
              "imagenFichero" => array:1 [
                0 => "xTab1213263.png"
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        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0060" class="elsevierStyleSimplePara elsevierViewall">Allelic and genotypic frequency of the <span class="elsevierStyleItalic">Ala-9Val</span> polymorphism of the <span class="elsevierStyleItalic">MnSOD</span> gene in patients and controls&#46;</p>"
        ]
      ]
      2 => array:8 [
        "identificador" => "tbl0015"
        "etiqueta" => "Table 3"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "detalles" => array:1 [
          0 => array:3 [
            "identificador" => "at3"
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          "leyenda" => "<p id="spar0080" class="elsevierStyleSimplePara elsevierViewall">AD&#58; Alzheimer disease&#59; 95&#37; CI&#58; confidence interval&#59; ns&#58; not significant&#59; OR&#58; odds ratio&#46;</p><p id="spar0085" class="elsevierStyleSimplePara elsevierViewall">Values in parentheses indicate the number of individuals bearing the genotype&#46; Frequencies are expressed as percentages&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">AD<br><span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>79&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Controls<br><span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>100&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">OR<br>95&#37; CI&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">P</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " colspan="5" align="left" valign="top"><span class="elsevierStyleItalic">Genotype</span></td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">¿</span>2&#47;<span class="elsevierStyleItalic">¿</span>3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0 &#40;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">12 &#40;12&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;04&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;0007&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">¿</span>2&#47;<span class="elsevierStyleItalic">¿</span>4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;3 &#40;1&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0 &#40;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&#46;84&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">ns&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">¿</span>3&#47;<span class="elsevierStyleItalic">¿</span>3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">45&#46;6 &#40;36&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">60 &#40;60&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#46;55&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;02&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">¿</span>3&#47;<span class="elsevierStyleItalic">¿</span>4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">37&#46;9 &#40;30&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">24 &#40;24&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;93&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;02&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">¿</span>4&#47;<span class="elsevierStyleItalic">¿</span>4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">15&#46;2 &#40;12&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4 &#40;4&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&#46;29&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;004&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="5" align="left" valign="top"><span class="elsevierStyleVsp" style="height:0.5px"></span></td></tr><tr title="table-row"><td class="td" title="table-entry  " colspan="5" align="left" valign="top"><span class="elsevierStyleItalic">Alleles</span></td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">¿</span>2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;6 &#40;1&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">6 &#40;12&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;04&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;00002&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">¿</span>3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">64&#46;6 &#40;102&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">78 &#40;156&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#46;513&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;0024&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">¿</span>4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">34&#46;8 &#40;55&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">16 &#40;32&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&#46;8&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;000019&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
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                0 => "xTab1213265.png"
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        "descripcion" => array:1 [
          "en" => "<p id="spar0075" class="elsevierStyleSimplePara elsevierViewall">Frequencies of genotypes and alleles of the <span class="elsevierStyleItalic">APOE</span> gene in patients with AD and controls&#46;</p>"
        ]
      ]
      3 => array:8 [
        "identificador" => "tbl0020"
        "etiqueta" => "Table 4"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "detalles" => array:1 [
          0 => array:3 [
            "identificador" => "at4"
            "detalle" => "Table "
            "rol" => "short"
          ]
        ]
        "tabla" => array:2 [
          "leyenda" => "<p id="spar0095" class="elsevierStyleSimplePara elsevierViewall">AD&#58; Alzheimer disease&#59; 95&#37; CI&#58; confidence interval&#59; OR&#58; odds ratio&#59; <span class="elsevierStyleItalic">&#967;</span><span class="elsevierStyleSup">2</span>&#58; chi-square test&#46;</p><p id="spar0100" class="elsevierStyleSimplePara elsevierViewall">Values in parentheses indicate the number of individuals bearing the genotype&#46; Frequencies are expressed as percentages&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">AD<br><span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>79&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Controls<br><span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>100&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">OR<br>95&#37; CI&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">P</span> &#40;<span class="elsevierStyleItalic">&#967;</span><span class="elsevierStyleSup">2</span>&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " colspan="5" align="left" valign="top"><span class="elsevierStyleItalic">Genotype</span></td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#43;&#47;¿2¿3</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0 &#40;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">5 &#40;5&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">0&#46;10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;02&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#43;&#47;¿3¿3</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">14 &#40;11&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">26 &#40;26&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">0&#46;46&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;02&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#8722;&#47;¿3¿4</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">14 &#40;11&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">5 &#40;5&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">3&#46;07&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;019&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">GSTT1&#43;&#47;GSTM1&#8722;&#47;¿4¿4</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">10 &#40;8&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2 &#40;2&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">5&#46;52&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;009&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
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                0 => "xTab1213262.png"
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        "descripcion" => array:1 [
          "en" => "<p id="spar0090" class="elsevierStyleSimplePara elsevierViewall">Frequencies of genotype combinations for <span class="elsevierStyleItalic">GSTT1&#47;GSTM1&#47;APOE</span> in patients with AD and controls&#46;</p>"
        ]
      ]
      4 => array:8 [
        "identificador" => "tbl0025"
        "etiqueta" => "Table 5"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "detalles" => array:1 [
          0 => array:3 [
            "identificador" => "at5"
            "detalle" => "Table "
            "rol" => "short"
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        ]
        "tabla" => array:2 [
          "leyenda" => "<p id="spar0110" class="elsevierStyleSimplePara elsevierViewall">AD&#58; Alzheimer disease&#59; 95&#37; CI&#58; confidence interval&#59; OR&#58; odds ratio&#59; <span class="elsevierStyleItalic">&#967;</span><span class="elsevierStyleSup">2</span>&#58; chi-square test&#46;</p><p id="spar0115" class="elsevierStyleSimplePara elsevierViewall">Values in parentheses show the number of individuals bearing the genotype for the polymorphic site under study&#46; Frequencies are expressed as percentages&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:2 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">AD<br><span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>79&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Controls<br><span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>100&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">OR<br>95&#37; CI&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">P</span> &#40;<span class="elsevierStyleItalic">&#967;</span><span class="elsevierStyleSup">2</span>&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2 &#40;2&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#46;05&nbsp;\t\t\t\t\t\t\n
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          "en" => "<p id="spar0105" class="elsevierStyleSimplePara elsevierViewall">Frequencies of genotype combinations of the <span class="elsevierStyleItalic">MnSOD&#47;APOE</span> gene in patients with AD and controls&#46;</p>"
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