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First report of Toxocara cati in the domestic land snail Rumina decollata
Primer reporte de Toxocara cati en el caracol doméstico Rumina decollata
Natalia Cardilloa,
Corresponding author
ncardillo@fvet.uba.ar

Corresponding author.
, Cintia Gonzalez Prousb, Silvio Krivokapichb, Mariana Pittaroa, Mariano Ercolea, Matías Pereza, Mariana Pasqualettia, Fernando Fariñaa, Adriana Rosaa, Graciana Gattib, Mabel Ribicicha
a Cátedra de Parasitología y Enfermedades Parasitarias, Facultad de Ciencias Veterinarias, Universidad de Buenos Aires, CONICET, Av. Chorroarin 280, C.P. C1427CWO Buenos Aires, Argentina
b Instituto Nacional de Enfermedades Infecciosas, ANLIS “Carlos G Malbrán”, Argentina
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          "en" => "<p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">DNA amplification of <span class="elsevierStyleItalic">Toxocara</span> spp&#46; isolated from <span class="elsevierStyleItalic">R&#46; decollata</span> snails by PCR on 2&#46;5&#37; agarose gel&#44; using the pair of primers 5&#8242;-ACGTATGCGTGAGCCG-3&#8242; and 5&#8242;-GTGTTTTTGGTTTTTGGCG-3&#8242;&#58; lane 1&#58; marker 50<span class="elsevierStyleHsp" style=""></span>bp&#59; lane 2&#58; negative control&#59; lane 3&#8211;14&#58; amplification of DNA from different isolated larvae&#44; each lane corresponds to a single larva&#59; lane 15&#58; DNA from <span class="elsevierStyleItalic">T&#46; cati</span> larvae isolated from cats feces&#44; positive control&#46;</p>"
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    "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Toxocara</span> spp&#46;&#44; the common roundworms of dogs and cats&#44; produce eggs that are eliminated with their feces to the environment&#44; which become a source of infection for paratenic hosts like mammals &#40;including humans&#41;&#44; birds and invertebrates<a class="elsevierStyleCrossRef" href="#bib0095"><span class="elsevierStyleSup">4</span></a>&#46; <span class="elsevierStyleItalic">Toxocara</span> spp&#46; larvae are able to migrate through the tissues of their hosts&#44; and in man cause human Toxocariasis<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">12</span></a>&#46; In some countries there are cultural dietary preferences for raw or undercooked meat of paratenic hosts and it became a risk factor for acquiring the human disease&#46; Most human infections are asymptomatic but the infection may lead in clinical syndromes known as visceral larva migrans &#40;VLM&#41;&#44; ocular larva migrans &#40;OLM&#41; and common or covert toxocariasis &#40;CT&#41;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">12</span></a>&#46;</p><p id="par0010" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Rumina decollata</span> &#40;Linnaeus&#44; 1758&#41; is a pulmonate land snail native to southern Europe&#44; northern Africa and western Asia&#44; and it borders the Mediterranean Sea&#46; In America&#44; it was accidentally and intentionally introduced as biological control of the garden snail <span class="elsevierStyleItalic">Helix aspersa</span><a class="elsevierStyleCrossRef" href="#bib0085"><span class="elsevierStyleSup">2</span></a>&#46; In Argentina&#44; it has been recorded in urban areas of Buenos Aires&#44; Mendoza and La Pampa provinces<a class="elsevierStyleCrossRef" href="#bib0085"><span class="elsevierStyleSup">2</span></a>&#46; The snail is omnivorous&#59; they also prey upon other land snails&#44; eggs&#44; worms and insects and feed from decaying fresh vegetable and organic matter like animal faeces<a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">5</span></a>&#46;</p><p id="par0015" class="elsevierStylePara elsevierViewall">This study was aimed at reporting the presence of <span class="elsevierStyleItalic">T&#46; cati</span> in a stray cat population living in a public institution of Buenos Aires city and the evidence of infection in <span class="elsevierStyleItalic">R&#46; decollata</span> snail&#44; as potential paratenic host of the parasite in the same environment&#46;</p><p id="par0020" class="elsevierStylePara elsevierViewall">The study site was the open spaces of a public Hospital from the city of Buenos Aires&#44; inhabited by a feline stray population&#46; The general characteristics of the surrounding fences do not allow the entrance of dogs but permit cats&#8217; access&#46; Seventeen samples of cats&#8217; feces were collected individually from the environment of the open spaces surrounding the buildings of this public Institution&#46; They were stored at 4<span class="elsevierStyleHsp" style=""></span>&#176;C before being processed&#46; <span class="elsevierStyleItalic">R&#46; decollata</span> snails were identified according to the descriptions of Dundee<a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">5</span></a>&#44; and 75 adult specimens were collected from the same environment and were kept in plastic bags<a class="elsevierStyleCrossRef" href="#bib0085"><span class="elsevierStyleSup">2</span></a>&#46;</p><p id="par0025" class="elsevierStylePara elsevierViewall">Fifteen grams of each cat sample feces was weighted and processed by Benbrook&#39;s technique&#44; with modification according to Dolcetti<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">3</span></a>&#44; <span class="elsevierStyleItalic">T&#46; cati</span> eggs were identified under optic microscope &#40;10&#215;&#41;&#44; according to Sprent<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">14</span></a> descriptions&#46; <span class="elsevierStyleItalic">R&#46; decollata</span> snails were processed in 25 pools of three adult snails each one&#44; since its low weight made it difficult for individual processing&#46; They were cleaned individually by brushing the foot and shell in order to remove free living nematodes attached or their eggs&#46; Snails were killed by immersion in tepid water for 24<span class="elsevierStyleHsp" style=""></span>h&#59; shells were removed and bodies were weighed&#44; minced with scissors and processed by artificial digestion technique<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">7</span></a>&#46; Each snail macerated pool was placed in an Erlenmeyer with the digestive fluid &#40;per 1<span class="elsevierStyleHsp" style=""></span>g of snail tissue it was used 0&#46;15<span class="elsevierStyleHsp" style=""></span>g&#44; pepsin&#44; 0&#46;15<span class="elsevierStyleHsp" style=""></span>ml concentrated HCl and 15<span class="elsevierStyleHsp" style=""></span>ml tap water&#41;&#44; incubated in a magnetic shaker &#40;1000<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">g</span>&#41;&#44; for one hour&#44; at 37<span class="elsevierStyleHsp" style=""></span>&#176;C&#46; Afterwards&#44; the fluid of the digestion was filtered in a 500<span class="elsevierStyleHsp" style=""></span>&#956; mesh and centrifuged at 1500<span class="elsevierStyleHsp" style=""></span>rpm for three minutes&#46; The supernatant was discharged and total larvae were counted under the light microscope &#40;10&#215;&#41;&#46; Larvae were identified according to morphological characteristics described by Sprent<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">14</span></a>&#44; like the anterior end with a mouth dorsally inclined and a spine-like cuticular thickening forms the ventral margin of a shallow buccal capsule<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">14</span></a>&#46;</p><p id="par0030" class="elsevierStylePara elsevierViewall">Polymerase chain reaction &#40;PCR&#41; amplification of larval DNA was carried out to confirm <span class="elsevierStyleItalic">Toxocara</span> spp&#46; infection&#46; DNA isolation and extraction was performed using lysis buffer and proteinase K for two hours at 65<span class="elsevierStyleHsp" style=""></span>&#176;C&#46; The PCR mixture &#40;total volume of 25<span class="elsevierStyleHsp" style=""></span>&#956;l&#41; consisted of 2&#46;5<span class="elsevierStyleHsp" style=""></span>&#956;l of 5<span class="elsevierStyleHsp" style=""></span>&#956;l of a single larvae preparation&#44; 2&#46;5<span class="elsevierStyleHsp" style=""></span>&#956;l of 10&#215; PCR Buffer&#44; 0&#46;2<span class="elsevierStyleHsp" style=""></span>&#956;l of 2&#46;5<span class="elsevierStyleHsp" style=""></span>mM each deoxynucleotide triphosphate&#44; 2<span class="elsevierStyleHsp" style=""></span>U Taq polymerase and 1<span class="elsevierStyleHsp" style=""></span>&#956;l &#40;6&#46;25<span class="elsevierStyleHsp" style=""></span>&#956;M&#41; of two pair of primers designed in silico by Gonz&#225;lez Prous et al&#46;<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">8</span></a> &#40;5&#8242;-ACGTATGCGTGAGCCG-3&#8242; and 5&#8242;-GTGTTTTTGGTTTTTGGCG-3&#8242;&#41;&#46; The bioinformatic analysis was performed using ITS-1 from ribosomal DNA from <span class="elsevierStyleItalic">T&#46; canis</span> and <span class="elsevierStyleItalic">T&#46; cati</span> obtained from the public database&#44; GenBank&#46; It has been designed in-silico primers in order to amplify a region that could&#44; after sequencing&#44; reveals species of <span class="elsevierStyleItalic">Toxocara</span>&#46; Direct sequencing was performed on an automated Sequencer&#44; using forward and reverse primers and the Big Dye Terminator kit from Applied Biosystems&#44; according to the manufacturer&#39;s instructions&#46; Sequences were analyzed by alignment with reference sequences from Basic Local Alignment Search Tool &#40;BLAST&#41; algorithm optimized for highly similar sequences &#40;megablast&#41; at the National Center for Biotechnology Information &#40;NCBI&#44; Bethesda&#44; MD&#44; USA&#41; &#40;<a id="intr0005" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/blast/">www&#46;ncbi&#46;nlm&#46;nih&#46;gov&#47;blast&#47;</a>&#41;&#46;</p><p id="par0035" class="elsevierStylePara elsevierViewall">DNA was amplified for 35 cycles&#44; each cycle consisted of 1<span class="elsevierStyleHsp" style=""></span>min at 94<span class="elsevierStyleHsp" style=""></span>&#176;C&#44; 1<span class="elsevierStyleHsp" style=""></span>min at 55<span class="elsevierStyleHsp" style=""></span>&#176;C&#44; and 1<span class="elsevierStyleHsp" style=""></span>min at 72<span class="elsevierStyleHsp" style=""></span>&#176;C&#46; Previously to the first cycle and after the final cycle longer denaturation &#40;3<span class="elsevierStyleHsp" style=""></span>min at 95<span class="elsevierStyleHsp" style=""></span>&#176;C&#41; and extension &#40;10<span class="elsevierStyleHsp" style=""></span>min at 72<span class="elsevierStyleHsp" style=""></span>&#176;C&#41; steps were applied&#44; respectively&#46; Amplification products were visualized after electrophoresis on 2&#46;5&#37; agarose gel and visualized under an UV transiluminator after staining with ethidium bromide&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall">Prevalence of <span class="elsevierStyleItalic">T&#46; cati</span> positive feces was calculated&#46; Proportion of <span class="elsevierStyleItalic">R&#46; decollata</span> positive pools and mean value of <span class="elsevierStyleItalic">T&#46; cati</span> total larvae &#40;L3&#41; per gram and per pool of snail was reported&#46; The estimation of the confidence intervals &#40;CI&#41; was created at the 95&#37; confidence level&#46; Statistical analysis was performed using InfoStat software&#46;</p><p id="par0045" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">T&#46; cati</span> larval eggs were recovered in 23&#46;5&#37; &#40;4&#47;17&#41; of the cat sample feces collected&#46; Most of <span class="elsevierStyleItalic">R&#46; decollata</span> snails were found on buried feces &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>&#41;&#46; Third <span class="elsevierStyleItalic">T&#46; cati</span> larval stages &#40;L3&#41; were recovered from the 20&#37; &#40;5&#47;25&#41; of snails&#8217; pools &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>&#41;&#46; The mean value of <span class="elsevierStyleItalic">T&#46; cati</span> total larvae per gram of snail recovered in total positive pools was 5&#46;1 larvas &#40;CI 95&#37;&#58; &#8722;2&#46;5 to 12&#46;7&#41;&#44; Mean value of total L3&#47;pool was 8&#46;4 &#40;CI 95&#37;&#58; &#8722;8&#46;8 to 25&#46;6&#41;&#44; and the maximum was 33 L3&#47;pool&#46; There have been sequenced two amplicons of 297<span class="elsevierStyleHsp" style=""></span>bp in length &#40;<a class="elsevierStyleCrossRef" href="#fig0015">Fig&#46; 3</a>&#41; from individual larvae that were deposited into the GenBank database under Accession Nos&#46; KR337264 and KR337265&#46; BLASTN searches against GenBank reference sequences for <span class="elsevierStyleItalic">T&#46; cati</span> with a query cover of 100&#37; displayed high degrees of similarities &#40;100&#8211;98&#37;&#41;&#46; The DNA sequence GenBank Accession No KR337264 in study revealed the highest nucleotide identity to <span class="elsevierStyleItalic">T&#46; cati</span> &#40;100&#37;&#44; GenBank Accesi&#243;n No&#46; <a id="intr0010" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=KJ777179">KJ777179</a>&#59; GenBank Accesi&#243;n No&#46; <a id="intr0015" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=AB571303">AB571303</a>&#59; GenBank Accesi&#243;n No&#46; <a id="intr0020" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=AB110025">AB110025</a>&#59; 99&#37;<span class="elsevierStyleHsp" style=""></span>&#44; GenBank Accesi&#243;n No&#46; <a id="intr0025" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=KJ777163">KJ777163</a>&#59; GenBank Accesi&#243;n No&#46; <a id="intr0030" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=JF837172">JF837172</a>&#59; GenBank Accesi&#243;n No&#46; <a id="intr0035" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=AJ002436">AJ002436</a>&#59; 98&#37;&#44; GenBank Accesi&#243;n No&#46; <a id="intr0040" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=AJ002437">AJ002437</a>&#41;&#44; whereas the nucleotide sequence GenBank Accession No KR337265 showed a slightly lower similarity to this <span class="elsevierStyleItalic">Toxocara</span> species &#40;99&#37;&#44; GenBank Accesi&#243;n No&#46; <a id="intr0045" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=KJ777179">KJ777179</a>&#59; GenBank Accesi&#243;n No&#46; <a id="intr0050" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=AB571303">AB571303</a>&#59; GenBank Accesi&#243;n No&#46; <a id="intr0055" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=AB110025">AB110025</a>&#44; GenBank Accesi&#243;n No&#46; <a id="intr0060" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=KJ777163">KJ777163</a>&#44; GenBank Accesi&#243;n No&#46; <a id="intr0065" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=JF837172">JF837172</a>&#44; GenBank Accesi&#243;n No&#46; <a id="intr0070" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=AJ002436">AJ002436</a>&#59; 98&#37;&#44; GenBank Accesi&#243;n No&#46; <a id="intr0075" class="elsevierStyleInterRef" href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=search&db=nucleotide&doptcmdl=genbank&term=AJ002437">AJ002437</a>&#41;&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><elsevierMultimedia ident="fig0010"></elsevierMultimedia><elsevierMultimedia ident="fig0015"></elsevierMultimedia><p id="par0050" class="elsevierStylePara elsevierViewall">In the city of Buenos Aires&#44; there are free cats populations living in semi-wild conditions in public institutions which offer them shelter and protection<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">13</span></a>&#46; The presence of <span class="elsevierStyleItalic">T&#46; cati</span> &#40;23&#46;5&#37;&#41; in cats feces&#44; confirmed that the life cycle of the parasite takes place in the area&#46; Most of snails were found feeding on buried feces so coprophagus habits of <span class="elsevierStyleItalic">R&#46; decollata</span> were confirm&#46; It seems that feces provide food for snails and protective substrate for <span class="elsevierStyleItalic">T&#46; cati</span> eggs by perpetuating the snail and parasite cycle in contaminated environments&#46; These conditions would represent a risk for rodents and birds as paratenic hosts and also for cats&#46; Dubinsky et al<span class="elsevierStyleItalic">&#46;</span><a class="elsevierStyleCrossRef" href="#bib0095"><span class="elsevierStyleSup">4</span></a> reported the importance of earthworms and mice as natural reservoirs of <span class="elsevierStyleItalic">Toxocara</span> spp&#46; larvae in nature&#44; even more&#44; as sentinels of environmental contamination&#44; especially in urban areas&#44; representing an important source of infection for hunter cats&#46;</p><p id="par0055" class="elsevierStylePara elsevierViewall">Sprent<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">14</span></a> recovered <span class="elsevierStyleItalic">T&#46; cati</span> larvae by experimental infection of earthworms and cockroaches&#46; Mizgajska et al&#46;<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">10</span></a>&#44; obtained an 87&#37; of earthworms&#44; collected from urban gardens and courtyards&#44; positive to <span class="elsevierStyleItalic">Toxocara</span> spp&#46; eggs&#46; Umeche et al&#46;<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">15</span></a> found <span class="elsevierStyleItalic">Toxocara</span> spp&#46; eggs in domestic flies captured in Nigeria&#46;</p><p id="par0060" class="elsevierStylePara elsevierViewall">Human cases of toxocariasis acquired by eating raw snails were reported in Spain<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">11</span></a> and in Italy<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">1</span></a>&#46; In all cases&#44; the snail species involved probably was <span class="elsevierStyleItalic">H&#46; aspers</span>&#59; since there is a popular belief in eating raw snails as a good treatment and prevention of gastric ulcer so it was traditionally medicinally used<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">1</span></a>&#46; Romeu et al<span class="elsevierStyleItalic">&#46;</span><a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">11</span></a> suggested that the likely mechanism of transmission might have been the ingestion of <span class="elsevierStyleItalic">Toxocara</span> spp&#46; eggs mechanically attached to snail bodies&#44; eaten raw without a thorough cleaning&#46;</p><p id="par0065" class="elsevierStylePara elsevierViewall">Molecular identification revealed the highest nucleotide identity to <span class="elsevierStyleItalic">T&#46; cati</span> &#40;100&#8211;98&#37;&#41;&#44; and was coincident with that previously reported<a class="elsevierStyleCrossRefs" href="#bib0105"><span class="elsevierStyleSup">6&#44;9</span></a>&#46; Specific PCR assays were both sensitive and specific&#44; and provide molecular tools for diagnosis and epidemiological surveys with species-specific primers designed&#44; using the ITS-1 and ITS-2 sequences for the identification and differentiation of dog and cat <span class="elsevierStyleItalic">Toxocara</span> species<a class="elsevierStyleCrossRefs" href="#bib0105"><span class="elsevierStyleSup">6&#44;9</span></a>&#46; The increase of cats&#8217; populations which live in semi-wild conditions in urban areas without health care needs special attention from sanitary authorities&#46; They may consider the role of these cats in the circulation of emerging pathogens and as reservoirs of other diseases&#44; which include some zoonotic infections like toxocariasis&#46; Moreover&#44; it is necessary to improve epidemiological surveillance of ecological species such as <span class="elsevierStyleItalic">R&#46; decollata</span>&#44; which could become intermediate hosts of animals&#8217; parasites and contribute to maintain them in the environment&#46;</p><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Ethical disclosures</span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Protection of human and animal subjects</span><p id="par0070" class="elsevierStylePara elsevierViewall">The authors declare that no experiments were performed on humans or animals for this study&#46;</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Confidentiality of data</span><p id="par0075" class="elsevierStylePara elsevierViewall">The authors declare that no patient data appear in this article&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Right to privacy and informed consent</span><p id="par0080" class="elsevierStylePara elsevierViewall">The authors declare that no patient data appear in this article&#46;</p></span></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Conflict of interest</span><p id="par0085" class="elsevierStylePara elsevierViewall">The authors declare that they have no conflicts of interest&#46;</p></span></span>"
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          "titulo" => "Resumen"
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          "titulo" => "Ethical disclosures"
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              "identificador" => "sec0010"
              "titulo" => "Protection of human and animal subjects"
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            1 => array:2 [
              "identificador" => "sec0015"
              "titulo" => "Confidentiality of data"
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            2 => array:2 [
              "identificador" => "sec0020"
              "titulo" => "Right to privacy and informed consent"
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        5 => array:2 [
          "identificador" => "sec0025"
          "titulo" => "Conflict of interest"
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        6 => array:1 [
          "titulo" => "References"
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    "fechaRecibido" => "2015-11-19"
    "fechaAceptado" => "2016-04-15"
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          "clase" => "keyword"
          "titulo" => "Keywords"
          "identificador" => "xpalclavsec740648"
          "palabras" => array:3 [
            0 => "<span class="elsevierStyleItalic">Toxocara cati</span>"
            1 => "<span class="elsevierStyleItalic">Rumina decollata</span>"
            2 => "Paratenic host"
          ]
        ]
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        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palabras clave"
          "identificador" => "xpalclavsec740649"
          "palabras" => array:3 [
            0 => "<span class="elsevierStyleItalic">Toxocara cati</span>"
            1 => "<span class="elsevierStyleItalic">Rumina decollata</span>"
            2 => "Hospedador parat&#233;nico"
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        "titulo" => "Abstract"
        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">The prospective role of the land snail <span class="elsevierStyleItalic">Rumina decollata</span> as a potential paratenic host of <span class="elsevierStyleItalic">Toxocara cati</span> for domestic cats was studied&#46; <span class="elsevierStyleItalic">R&#46; decollata</span> specimens and cats&#8217; feces were collected from the open spaces of a Buenos Aires city hospital&#46; Cats&#8217; feces were analyzed and snails were digested to identify <span class="elsevierStyleItalic">T&#46; cati</span> stages&#44; by morphological and molecular analyses&#46; <span class="elsevierStyleItalic">T&#46; cati</span> larval eggs were recovered from 23&#46;5&#37; &#40;4&#47;17&#41; of the sampled feces&#46; Twenty percent of snail pools &#40;5&#47;25&#41; were confirmed to be positive for <span class="elsevierStyleItalic">Toxocara</span> spp&#46; third larval stage &#40;L3&#41; by PCR&#46; The mean value of total larvae recovered per gram of snail in all positive pools was 5&#46;1&#44; with a maximum 33 L3&#47;pool&#46; This is the first report of <span class="elsevierStyleItalic">T&#46; cati</span> infective larvae in <span class="elsevierStyleItalic">R&#46; decollata</span> domestic snail as a paratenic host&#44; since the relationship between infection in snails and in cats&#8217; feces could be demonstrated in a common environment&#46;</p></span>"
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        "titulo" => "Resumen"
        "resumen" => "<span id="abst0010" class="elsevierStyleSection elsevierViewall"><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Se estudi&#243; el rol prospectivo de <span class="elsevierStyleItalic">Rumina decollata</span> como potencial hospedador parat&#233;nico de <span class="elsevierStyleItalic">Toxocara cati</span> para los gatos dom&#233;sticos&#46; Se recolectaron caracoles <span class="elsevierStyleItalic">R&#46; decollata</span> y heces de gatos de un hospital de la Ciudad Aut&#243;noma de Buenos Aires&#46; Se procesaron las heces y los caracoles fueron digeridos para identificar estadios de <span class="elsevierStyleItalic">T&#46; cati</span> por an&#225;lisis morfol&#243;gico y molecular&#46; El 23&#44;5&#37; &#40;4&#47;17&#41; de las muestras de heces resultaron positivas a huevos larvados de <span class="elsevierStyleItalic">T&#46; cati</span>&#46; El 20&#37; &#40;5&#47;25&#41; de los pooles de caracoles fue positivo a larvas de tercer estad&#237;o &#40;L3&#41; de <span class="elsevierStyleItalic">Toxocara</span> spp&#46; por PCR&#46; El promedio de larvas totales recuperadas por gramo de caracol en todos los pooles positivos fue de 5&#46;1&#44; con un m&#225;ximo de 33 L3&#47;pool&#46; Se trata del primer reporte de <span class="elsevierStyleItalic">R&#46; decollata</span> como hospedador parat&#233;nico de <span class="elsevierStyleItalic">T&#46; cati</span>&#44; puesto que ha sido demostrada la infecci&#243;n en caracoles y gatos en un ambiente com&#250;n&#46;</p></span>"
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          "en" => "<p id="spar0015" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">R&#46; decollata</span> snails feeding on buried feces from the open spaces of a public hospital&#44; Autonomous City of Buenos Aires&#44; Argentina&#46;</p>"
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          "en" => "<p id="spar0020" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">T&#46; cati</span> larva 3 obtained from digested snails collected from the open spaces of a public hospital&#44; Autonomous city of Buenos Aires&#44; Argentina&#46; The arrow shows the anterior end with a mouth dorsally inclined &#40;45&#215;&#41;&#46;</p>"
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          "en" => "<p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">DNA amplification of <span class="elsevierStyleItalic">Toxocara</span> spp&#46; isolated from <span class="elsevierStyleItalic">R&#46; decollata</span> snails by PCR on 2&#46;5&#37; agarose gel&#44; using the pair of primers 5&#8242;-ACGTATGCGTGAGCCG-3&#8242; and 5&#8242;-GTGTTTTTGGTTTTTGGCG-3&#8242;&#58; lane 1&#58; marker 50<span class="elsevierStyleHsp" style=""></span>bp&#59; lane 2&#58; negative control&#59; lane 3&#8211;14&#58; amplification of DNA from different isolated larvae&#44; each lane corresponds to a single larva&#59; lane 15&#58; DNA from <span class="elsevierStyleItalic">T&#46; cati</span> larvae isolated from cats feces&#44; positive control&#46;</p>"
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es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos