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Letter to the Editor
Bacillus thuringiensis-based biopesticides, are they as effective as they should be?
¿Son los biopesticidas basados en Bacillus thuringiensis tan efectivos como deberían?
Leopoldo Palmaa,b
a Centro de Investigaciones y Transferencia de Villa María (CITVM-CONICET), Universidad Nacional de Villa María, (5900) Villa María, Córdoba, Argentina
b Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
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    "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Bacillus thuringiensis</span> synthesizes a number of invertebrate toxins that are mainly active against insects and has demonstrated its potential and safety as a biocontrol agent for decades<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">3</span></a>&#46; These proteins include crystal &#40;Cry and Cyt&#41; and vegetative &#40;secretable&#41; insecticidal proteins &#40;Vip&#41; that are highly toxic against insects<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">3</span></a>&#46; To date&#44; <span class="elsevierStyleItalic">B&#46; thuringiensis</span>-based biopesticides represent a clear alternative to chemical insecticides and account for about 80&#37; of all biopesticides marketed worldwide<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">4</span></a>&#46; Chemical insecticides contaminate water and food sources&#44; are harmful for non-target organisms and generate insect resistance&#46; <span class="elsevierStyleItalic">B&#46; thuringiensis</span>-based biopesticides are biodegradable and specific for their targets&#46; In fact&#44; their biodegradability becomes their main disadvantage since their active ingredients&#44; the insecticidal crystal proteins&#44; are susceptible to natural abiotic factors such as pH&#44; temperature and sunlight&#46; This disadvantage has stimulated the development of different encapsulation approaches intended to protect and extend the shelf life of sprayable formulations&#46; The encapsulation of <span class="elsevierStyleItalic">B&#46; thuringiensis</span> toxins into recombinant bacteria is a convenient tool for enhancing their field persistence&#44; which deserves further investigations since it will allow not only to protect the active ingredient but also to concentrate secretable insecticidal proteins &#40;e&#46;g&#46; Vip3 and Cry1I&#41;<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">2</span></a>&#46; However&#44; in order to be successful&#44; the system of choice should meet the following requirements&#58; &#40;i&#41; a GRAS &#40;General Recognized As Safe&#41; bacterium should be used&#46; Several GRAS bacteria have been successfully used for the production of recombinant proteins &#40;e&#46;g&#46; <span class="elsevierStyleItalic">Bacillus megaterium</span> and <span class="elsevierStyleItalic">Bacillus subtilis</span>&#41;&#44; representing clear alternatives for the production of encapsulated insecticidal proteins&#59; &#40;ii&#41; the bacterial cell wall should remain intact as a natural microcapsule&#46; This will allow not only to protect the toxin but also the intracellular concentration of secretable insecticidal proteins&#59; &#40;iii&#41; the protein should remain encapsulated into the cell maintaining its activity&#46; Bioassays must be performed in order to rule out loss of activity and to determine the digestibility of the bacterial cell wall by the insect and in comparison against non-encapsulated proteins&#59; &#40;iv&#41; this system should be capable of being produced at industrial scale and be competitive in the biopesticide market&#59; therefore&#44; expensive inductors such as IPTG &#40;isopropyl &#946;-<span class="elsevierStyleSmallCaps">d</span>-1-thiogalactopyranoside&#41; must be avoided&#59; &#40;vi&#41; the recombinant strains should not be capable of transferring recombinant DNA to wild type strains&#46; Some systems prevent &#8216;leaks&#8217; of recombinant DNA by using chemical methods that kill recombinant bacteria after protein expression &#40;e&#46;g&#46; fixation of recombinant cells with lugol&#41;<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">1</span></a>&#46; <span class="elsevierStyleItalic">B&#46; thuringiensis</span> has been the most used bacterium for the control of insect pests and human-disease vectors during the last forty years by means of the production of formulated pesticides and transgenic crops&#46; However&#44; the active ingredients of formulated <span class="elsevierStyleItalic">B&#46; thuringiensis</span> pesticides are susceptible to different environmental factors that diminish their activity&#44; also limiting their shelf life after application&#46; The improvement of their residual activity will not only allow to formulate secretable toxins but also stimulate their development and increase their presence in the global pesticide market&#44; which is currently as low as 2&#37;<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">5</span></a>&#46;</p></span>"
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Article information
ISSN: 03257541
Original language: English
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2018 April 5 2 7
2018 March 9 1 10
2018 February 9 1 10
2018 January 2 0 2
2017 December 9 0 9
2017 November 7 1 8
2017 October 8 4 12
2017 September 8 0 8
2017 August 8 4 12
2017 July 7 2 9
2017 June 21 3 24
2017 May 24 3 27
2017 April 13 5 18
2017 March 5 14 19
2017 February 7 13 20
2017 January 1 10 11
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es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos