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array:24 [ "pii" => "S0325754116300979" "issn" => "03257541" "doi" => "10.1016/j.ram.2016.10.003" "estado" => "S300" "fechaPublicacion" => "2017-01-01" "aid" => "144" "copyright" => "Asociación Argentina de Microbiología" "copyrightAnyo" => "2016" "documento" => "simple-article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "cor" "cita" => "Rev Argent Microbiol. 2017;49:119" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:2 [ "total" => 1260 "formatos" => array:3 [ "EPUB" => 37 "HTML" => 905 "PDF" => 318 ] ] "itemSiguiente" => array:19 [ "pii" => "S0325754116300992" "issn" => "03257541" "doi" => "10.1016/j.ram.2016.10.004" "estado" => "S300" "fechaPublicacion" => "2017-01-01" "aid" => "146" "copyright" => "Asociación Argentina de Microbiología" "documento" => "article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "sco" "cita" => "Rev Argent Microbiol. 2017;49:120-2" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:2 [ "total" => 999 "formatos" => array:3 [ "EPUB" => 60 "HTML" => 756 "PDF" => 183 ] ] "en" => array:11 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Microbiological image</span>" "titulo" => "Fungi observation in deteriorated wooden heritage: analysis using different imaging techniques" "tienePdf" => "en" "tieneTextoCompleto" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "120" "paginaFinal" => "122" ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Observación de hongos xilófagos en madera perteneciente al patrimonio histórico deteriorada: análisis utilizando diferentes técnicas de obtención de imágenes" ] ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0015" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 2606 "Ancho" => 2835 "Tamanyo" => 1080432 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Microscopy of deteriorated wood samples. Panel A: scanning electron microscopy (SEM) images of ultra slim cut of dark area observed in RX and CT, dense presence of hyphae. Panel B: SEM image of ultra slim cut of gray zone where microstructure loss and the presence of spores are observed. Panel C: Optical microscopy (OM) images of the dark area observed in RX and CT: altered wood appeared compartmentalized by dark secretions deposited in axial/radial parenchyma and in vessel and cell lumina. Panel D: OM images where generalized cell wall erosion is evident (arrowheads holes in vessel elements and erosion of wood cell walls are observed.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Paula V. Alfieri, María V. Correa" "autores" => array:2 [ 0 => array:2 [ "nombre" => "Paula V." "apellidos" => "Alfieri" ] 1 => array:2 [ "nombre" => "María V." "apellidos" => "Correa" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0325754116300992?idApp=UINPBA00004N" "url" => "/03257541/0000004900000001/v1_201704040030/S0325754116300992/v1_201704040030/en/main.assets" ] "itemAnterior" => array:19 [ "pii" => "S0325754116300876" "issn" => "03257541" "doi" => "10.1016/j.ram.2016.08.004" "estado" => "S300" "fechaPublicacion" => "2017-01-01" "aid" => "134" "copyright" => "Asociación Argentina de Microbiología" "documento" => "article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "fla" "cita" => "Rev Argent Microbiol. 2017;49:110-8" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:2 [ "total" => 6242 "formatos" => array:3 [ "EPUB" => 44 "HTML" => 5661 "PDF" => 537 ] ] "es" => array:12 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">ARTÍCULO ESPECIAL</span>" "titulo" => "<span class="elsevierStyleItalic">Blastocystis</span> spp.: avances, controversias y desafíos futuros" "tienePdf" => "es" "tieneTextoCompleto" => "es" "tieneResumen" => array:2 [ 0 => "es" 1 => "en" ] "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "110" "paginaFinal" => "118" ] ] "titulosAlternativos" => array:1 [ "en" => array:1 [ "titulo" => "<span class="elsevierStyleItalic">Blastocystis</span> spp.: Advances, controversies and future challenges" ] ] "contieneResumen" => array:2 [ "es" => true "en" => true ] "contieneTextoCompleto" => array:1 [ "es" => true ] "contienePdf" => array:1 [ "es" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Valeria F. del Coco, Nora B. Molina, Juan A. Basualdo, María A. Córdoba" "autores" => array:4 [ 0 => array:2 [ "nombre" => "Valeria F." "apellidos" => "del Coco" ] 1 => array:2 [ "nombre" => "Nora B." "apellidos" => "Molina" ] 2 => array:2 [ "nombre" => "Juan A." "apellidos" => "Basualdo" ] 3 => array:2 [ "nombre" => "María A." "apellidos" => "Córdoba" ] ] ] ] ] "idiomaDefecto" => "es" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0325754116300876?idApp=UINPBA00004N" "url" => "/03257541/0000004900000001/v1_201704040030/S0325754116300876/v1_201704040030/es/main.assets" ] "en" => array:14 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Letter to the Editor</span>" "titulo" => "<span class="elsevierStyleItalic">Bacillus thuringiensis</span>-based biopesticides, are they as effective as they should be?" "tieneTextoCompleto" => true "saludo" => "<span class="elsevierStyleItalic">Dear Editor:</span>" "paginas" => array:1 [ 0 => array:1 [ "paginaInicial" => "119" ] ] "autores" => array:1 [ 0 => array:3 [ "autoresLista" => "Leopoldo Palma" "autores" => array:1 [ 0 => array:4 [ "nombre" => "Leopoldo" "apellidos" => "Palma" "email" => array:1 [ 0 => "palma.leopoldo@gmail.com" ] "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] ] ] ] "afiliaciones" => array:2 [ 0 => array:3 [ "entidad" => "Centro de Investigaciones y Transferencia de Villa María (CITVM-CONICET), Universidad Nacional de Villa María, (5900) Villa María, Córdoba, Argentina" "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina" "etiqueta" => "b" "identificador" => "aff0010" ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "¿Son los biopesticidas basados en <span class="elsevierStyleItalic">Bacillus thuringiensis</span> tan efectivos como deberían?" ] ] "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Bacillus thuringiensis</span> synthesizes a number of invertebrate toxins that are mainly active against insects and has demonstrated its potential and safety as a biocontrol agent for decades<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">3</span></a>. These proteins include crystal (Cry and Cyt) and vegetative (secretable) insecticidal proteins (Vip) that are highly toxic against insects<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">3</span></a>. To date, <span class="elsevierStyleItalic">B. thuringiensis</span>-based biopesticides represent a clear alternative to chemical insecticides and account for about 80% of all biopesticides marketed worldwide<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">4</span></a>. Chemical insecticides contaminate water and food sources, are harmful for non-target organisms and generate insect resistance. <span class="elsevierStyleItalic">B. thuringiensis</span>-based biopesticides are biodegradable and specific for their targets. In fact, their biodegradability becomes their main disadvantage since their active ingredients, the insecticidal crystal proteins, are susceptible to natural abiotic factors such as pH, temperature and sunlight. This disadvantage has stimulated the development of different encapsulation approaches intended to protect and extend the shelf life of sprayable formulations. The encapsulation of <span class="elsevierStyleItalic">B. thuringiensis</span> toxins into recombinant bacteria is a convenient tool for enhancing their field persistence, which deserves further investigations since it will allow not only to protect the active ingredient but also to concentrate secretable insecticidal proteins (e.g. Vip3 and Cry1I)<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">2</span></a>. However, in order to be successful, the system of choice should meet the following requirements: (i) a GRAS (General Recognized As Safe) bacterium should be used. Several GRAS bacteria have been successfully used for the production of recombinant proteins (e.g. <span class="elsevierStyleItalic">Bacillus megaterium</span> and <span class="elsevierStyleItalic">Bacillus subtilis</span>), representing clear alternatives for the production of encapsulated insecticidal proteins; (ii) the bacterial cell wall should remain intact as a natural microcapsule. This will allow not only to protect the toxin but also the intracellular concentration of secretable insecticidal proteins; (iii) the protein should remain encapsulated into the cell maintaining its activity. Bioassays must be performed in order to rule out loss of activity and to determine the digestibility of the bacterial cell wall by the insect and in comparison against non-encapsulated proteins; (iv) this system should be capable of being produced at industrial scale and be competitive in the biopesticide market; therefore, expensive inductors such as IPTG (isopropyl β-<span class="elsevierStyleSmallCaps">d</span>-1-thiogalactopyranoside) must be avoided; (vi) the recombinant strains should not be capable of transferring recombinant DNA to wild type strains. Some systems prevent ‘leaks’ of recombinant DNA by using chemical methods that kill recombinant bacteria after protein expression (e.g. fixation of recombinant cells with lugol)<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">1</span></a>. <span class="elsevierStyleItalic">B. thuringiensis</span> has been the most used bacterium for the control of insect pests and human-disease vectors during the last forty years by means of the production of formulated pesticides and transgenic crops. However, the active ingredients of formulated <span class="elsevierStyleItalic">B. thuringiensis</span> pesticides are susceptible to different environmental factors that diminish their activity, also limiting their shelf life after application. The improvement of their residual activity will not only allow to formulate secretable toxins but also stimulate their development and increase their presence in the global pesticide market, which is currently as low as 2%<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">5</span></a>.</p></span>" "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2016-04-29" "fechaAceptado" => "2016-10-14" "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:5 [ 0 => array:3 [ "identificador" => "bib0030" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Recombinant cells of <span class="elsevierStyleItalic">Pseudomonas fluorescens</span>: a highly palatable encapsulation for delivery of genetically engineered toxins to subterranean termites (Isoptera: Rhinotermitidae)" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "J.K. Grace" 1 => "D.M. Ewart" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Lett Appl Microbiol" "fecha" => "1996" "volumen" => "23" "paginaInicial" => "183" "paginaFinal" => "186" ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib0035" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Encapsulation of the <span class="elsevierStyleItalic">Bacillus thuringiensis</span> secretable toxins Vip3 Aa and Cry1 Ia in <span class="elsevierStyleItalic">Pseudomonas fluorescens</span>" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "C.S. Hernández-Rodríguez" 1 => "I. Ruiz de Escudero" 2 => "A.C. Asensio" 3 => "J. Ferré" 4 => "P. Caballero" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Biol Control" "fecha" => "2013" "volumen" => "66" "paginaInicial" => "159" "paginaFinal" => "165" ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bib0040" "etiqueta" => "3" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "<span class="elsevierStyleItalic">Bacillus thuringiensis</span> and its pesticidal crystal proteins" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:8 [ 0 => "E. Schnepf" 1 => "N. Crickmore" 2 => "J. Van Rie" 3 => "D. Lereclus" 4 => "J. Baum" 5 => "J. Feitelson" 6 => "D.R. Zeigler" 7 => "D.H. Dean" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Microbiol Mol Biol Rev" "fecha" => "1998" "volumen" => "62" "paginaInicial" => "775" "paginaFinal" => "806" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9729609" "web" => "Medline" ] ] ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bib0045" "etiqueta" => "4" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Bt: mode of action and use" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "M.E. Whalon" 1 => "B.A. Wingerd" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1002/arch.10117" "Revista" => array:6 [ "tituloSerie" => "Arch Insect Biochem Physiol" "fecha" => "2003" "volumen" => "54" "paginaInicial" => "200" "paginaFinal" => "211" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/14635181" "web" => "Medline" ] ] ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bib0050" "etiqueta" => "5" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "pH-controlled <span class="elsevierStyleItalic">Bacillus thuringiensis</span> Cry1 Ac protoxin loading and release from polyelectrolyte microcapsules" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "W. Yang" 1 => "K. He" 2 => "J. Zhang" 3 => "S. Guo" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1371/journal.pone.0045233" "Revista" => array:5 [ "tituloSerie" => "PLoS One" "fecha" => "2012" "volumen" => "7" "paginaInicial" => "e45233" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23024810" "web" => "Medline" ] ] ] ] ] ] ] ] ] ] ] ] ] "idiomaDefecto" => "en" "url" => "/03257541/0000004900000001/v1_201704040030/S0325754116300979/v1_201704040030/en/main.assets" "Apartado" => array:4 [ "identificador" => "37864" "tipo" => "SECCION" "en" => array:2 [ "titulo" => "Carta al Editor" "idiomaDefecto" => true ] "idiomaDefecto" => "en" ] "PDF" => "https://static.elsevier.es/multimedia/03257541/0000004900000001/v1_201704040030/S0325754116300979/v1_201704040030/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0325754116300979?idApp=UINPBA00004N" ]
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