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Plasmid-mediated colistin resistance in Escherichia coli recovered from healthy poultry
Resistencia a colistina mediada por plásmido en Escherichia coli recuperadas de aves de corral sanas
Johana E. Domingueza,c, Roque A. Figueroa Espinosab,c, Leandro M. Redondoa,c, Daniela Cejasb,c, Gabriel O. Gutkindb,c,
, Pablo A. Chacanaa, José A. Di Conzab,c, Mariano E. Fernández-Miyakawaa,c
Corresponding author
a Instituto Nacional de Tecnología Agropecuaria, Centro Nacional de Investigaciones Agropecuarias, Instituto de Patobiología, Las Cabañas y los Reseros s/n, Casilla de Correo 25, 1712, Castelar, Provincia de Buenos Aires, Buenos Aires, Argentina
b Universidad de Buenos Aires, Facultad de Farmacia y Bioquímica, Junín 954, C1113AAD, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
c Consejo Nacional de Investigaciones Científicas y Tecnológicas, Godoy Cruz 2290, C1425FQB, Cuidad Autónoma de Buenos Aires, Buenos Aires, Argentina
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This use is currently under close scrutiny, as it may be – and for sure is – selecting and collaborating for multi-drug resistant enteric bacteria.</p><p id="par0010" class="elsevierStylePara elsevierViewall">As a service to poultry producers we have been monitoring the susceptibility to antimicrobial agents of indicator bacteria isolated from intensive farming systems to support choosing in advance the most effective promoters and to reduce selection pressure. <span class="elsevierStyleItalic">Escherichia coli</span> is one of the selected species considered markers for antimicrobial resistance evolution.</p><p id="par0015" class="elsevierStylePara elsevierViewall">From 2013 to date, we have obtained 304 <span class="elsevierStyleItalic">E. coli</span> isolates recovered from 129 broiler chicken farms (only poultry growers) located in several provinces of Argentina (Buenos Aires, Córdoba, Entre Rios, Rio Negro and Santa Fe). Isolates were recovered from fresh fecal samples collected randomly from clinically healthy chickens (aged 4–6 weeks). The analysis showed that almost half of them (49%) were found to be resistant to colistin, as determined by microdilution according to EUCAST breakpoint recommendations (version 6.0).</p><p id="par0020" class="elsevierStylePara elsevierViewall">Colistin is considered a last-line antimicrobial agent retaining activity on multiresistant bacteria recovered from humans. Even when resistance was sporadically reported, it was assumed to be obtained by mutation of regulatory genes<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">4</span></a>. However, a gene conferring resistance to colistin was recently reported in conjugative plasmids<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">2</span></a>. This gene (<span class="elsevierStyleItalic">mcr-1</span>) was most frequently found in <span class="elsevierStyleItalic">E. coli</span> but also in other species such as <span class="elsevierStyleItalic">Klebsiella pneumoniae</span> and <span class="elsevierStyleItalic">Salmonella</span><a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">1</span></a>. Up to date, a large number of publications demonstrated its presence in isolates collected mainly from animal samples, and to a lesser extent, in samples of human origin.</p><p id="par0025" class="elsevierStylePara elsevierViewall">After selecting approximately one third of our colistin-resistant <span class="elsevierStyleItalic">E. coli</span>, all of them were confirmed as <span class="elsevierStyleItalic">mcr-1</span> producers by PCR and full gene sequencing. In Argentina, the <span class="elsevierStyleItalic">mcr-1</span> determinant was previously reported in <span class="elsevierStyleItalic">E. coli</span> clinical strains isolated from inpatients<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">5</span></a>.</p><p id="par0030" class="elsevierStylePara elsevierViewall">It should be noted that in our country, many producers have voluntarily stopped using colistin after the technical reports of these (our) results, even before the initial report of plasmid-borne transmission. In this regard, the World Health Organization recommends that the use of colistin be limited for the treatment of clinically affected animals. Moreover, in our country, official regulations governing the administration of these compounds in animal feed have already changed, including a gradual stepwise process with a final goal to completely ban their use by 2019.</p></span>" "pdfFichero" => "main.pdf" "tienePdf" => true "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:5 [ 0 => array:3 [ "identificador" => "bib0030" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Detection of the plasmid-mediated <span class="elsevierStyleItalic">mcr-1</span> gene conferring colistin resistance in human and food isolates of <span class="elsevierStyleItalic">Salmonella enterica</span> and <span class="elsevierStyleItalic">Escherichia coli</span> in England and Wales" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:13 [ 0 => "M. Doumith" 1 => "G. 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Petroni" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1128/AAC.00573-16" "Revista" => array:6 [ "tituloSerie" => "Antimicrob Agents Chemother" "fecha" => "2016" "volumen" => "60" "paginaInicial" => "4412" "paginaFinal" => "4413" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/27090181" "web" => "Medline" ] ] ] ] ] ] ] ] ] ] ] ] ] "idiomaDefecto" => "en" "url" => "/03257541/0000004900000003/v1_201708180123/S0325754117300159/v1_201708180123/en/main.assets" "Apartado" => array:4 [ "identificador" => "37864" "tipo" => "SECCION" "en" => array:2 [ "titulo" => "Carta al Editor" "idiomaDefecto" => true ] "idiomaDefecto" => "en" ] "PDF" => "https://static.elsevier.es/multimedia/03257541/0000004900000003/v1_201708180123/S0325754117300159/v1_201708180123/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0325754117300159?idApp=UINPBA00004N" ]
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| 2020 April | 20 | 8 | 28 |
| 2020 March | 19 | 8 | 27 |
| 2020 February | 15 | 7 | 22 |
| 2020 January | 12 | 5 | 17 |
| 2019 December | 17 | 14 | 31 |
| 2019 November | 8 | 5 | 13 |
| 2019 October | 26 | 16 | 42 |
| 2019 September | 25 | 2 | 27 |
| 2019 August | 13 | 2 | 15 |
| 2019 July | 14 | 15 | 29 |
| 2019 June | 29 | 11 | 40 |
| 2019 May | 75 | 15 | 90 |
| 2019 April | 32 | 6 | 38 |
| 2019 March | 3 | 3 | 6 |
| 2019 February | 8 | 7 | 15 |
| 2019 January | 4 | 4 | 8 |
| 2018 December | 4 | 5 | 9 |
| 2018 November | 18 | 18 | 36 |
| 2018 October | 16 | 18 | 34 |
| 2018 September | 22 | 3 | 25 |
| 2018 August | 6 | 2 | 8 |
| 2018 July | 7 | 5 | 12 |
| 2018 June | 10 | 5 | 15 |
| 2018 May | 22 | 3 | 25 |
| 2018 April | 7 | 4 | 11 |
| 2018 March | 11 | 14 | 25 |
| 2018 February | 10 | 2 | 12 |
| 2018 January | 23 | 2 | 25 |
| 2017 December | 20 | 2 | 22 |
| 2017 November | 23 | 5 | 28 |
| 2017 October | 36 | 6 | 42 |
| 2017 September | 22 | 8 | 30 |
| 2017 August | 14 | 12 | 26 |
| 2017 July | 2 | 7 | 9 |
| 2017 June | 3 | 14 | 17 |
| 2017 May | 2 | 29 | 31 |
| 2017 April | 0 | 2 | 2 |
Show all
