Mycoplasma maculosum and Mycoplasma spumans associated with fertility disorders in dogs from a Bernese Mountain dog kennel

array:24 ["pii" => "S0325754121000523" "issn" => "03257541" "doi" => "10.1016/j.ram.2021.04.001" "estado" => "S300" "fechaPublicacion" => "2022-01-01" "aid" => "462" "copyright" => "Asociación Argentina de Microbiología" "copyrightAnyo" => "2021" "documento" => "article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "fla" "cita" => "Rev Argent Microbiol. 2022;54:39-42" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:1 [ "total" => 0 ] "itemSiguiente" => array:19 [ "pii" => "S0325754121000468" "issn" => "03257541" "doi" => "10.1016/j.ram.2021.02.011" "estado" => "S300" "fechaPublicacion" => "2022-01-01" "aid" => "456" "copyright" => "Asociación Argentina de Microbiología" "documento" => "article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "fla" "cita" => "Rev Argent Microbiol. 2022;54:43-7" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:1 [ "total" => 0 ] "en" => array:14 [ "idiomaDefecto" => true "cabecera" => "Brief report" "titulo" => "Clinical and epidemiological features of tuberculosis isolated from critically ill patients" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => array:3 [ 0 => "en" 1 => "en" 2 => "es" ] "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "43" "paginaFinal" => "47" ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Tuberculosis en la unidad de cuidados intensivos: características clínicas y epidemiológicas" ] ] "contieneResumen" => array:2 [ "en" => true "es" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 1804 "Ancho" => 3175 "Tamanyo" => 299112 ] ] "descripcion" => array:1 [ "en" => "Minimum spanning tree cluster analysis. (A) Samples in red correspond to ICU samples; samples in blue correspond to community samples. Samples of both groups were present in all clusters. (B) Same analysis colored by lineage. LAM and Haarlem lineages correspond to the most frequent isolates." ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Joaquín Hurtado, Cecilia Coitinho, Nicolás Nin, María Buroni, Francisco Javier Hurtado, Carlos Robello, Gonzalo Greif" "autores" => array:7 [ 0 => array:2 [ "nombre" => "Joaquín" "apellidos" => "Hurtado" ] 1 => array:2 [ "nombre" => "Cecilia" "apellidos" => "Coitinho" ] 2 => array:2 [ "nombre" => "Nicolás" "apellidos" => "Nin" ] 3 => array:2 [ "nombre" => "María" "apellidos" => "Buroni" ] 4 => array:2 [ "nombre" => "Francisco Javier" "apellidos" => "Hurtado" ] 5 => array:2 [ "nombre" => "Carlos" "apellidos" => "Robello" ] 6 => array:2 [ "nombre" => "Gonzalo" "apellidos" => "Greif" ] ] ] ] "resumen" => array:1 [ 0 => array:3 [ "titulo" => "Highlights" "clase" => "author-highlights" "resumen" => "<ul class="elsevierStyleList" id="lis0005"><li class="elsevierStyleListItem" id="lsti0005">•Genetic structure analysis of 60 TB strains isolated from patients from an ICU.</li><li class="elsevierStyleListItem" id="lsti0010">•We present a comparison with population circulating strains.</li><li class="elsevierStyleListItem" id="lsti0015">•We evaluate the correlation between the clinical outcome and genotypic data.</li><li class="elsevierStyleListItem" id="lsti0020">•We present an extensive cohort study done in critically ill patients with tuberculosis.</li></ul>" ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0325754121000468?idApp=UINPBA00004N" "url" => "/03257541/0000005400000001/v1_202204020607/S0325754121000468/v1_202204020607/en/main.assets" ] "itemAnterior" => array:19 [ "pii" => "S0325754121000444" "issn" => "03257541" "doi" => "10.1016/j.ram.2021.02.009" "estado" => "S300" "fechaPublicacion" => "2022-01-01" "aid" => "454" "copyright" => "Asociación Argentina de Microbiología" "documento" => "article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "fla" "cita" => "Rev Argent Microbiol. 2022;54:35-8" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:1 [ "total" => 0 ] "en" => array:13 [ "idiomaDefecto" => true "cabecera" => "Brief report" "titulo" => "Expanded distribution of orthohantavirus Pergamino genotype: First record in Entre Rios province, Argentina" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => array:2 [ 0 => "en" 1 => "es" ] "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "35" "paginaFinal" => "38" ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Ampliación de la distribución del Orthohantavirus genotipo Pergamino: Primer registro en la Provincia de Entre Ríos, Argentina" ] ] "contieneResumen" => array:2 [ "en" => true "es" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 1120 "Ancho" => 2341 "Tamanyo" => 162142 ] ] "descripcion" => array:1 [ "en" => "Molecular phylogenetic analysis by Maximum Likelihood method. Phylogenetic analysis of hantaviruses based on 853bp of S-segment genome of Andes virus, and other orthohantaviruses characterized previously. Taxa in the branches of the tree indicate virus name and province of origin. GenBank accession numbers and reservoir rodent specie were: AF482717, Pergamino (Akodon azarae); AF482716, Maciel (Necromys benefactus); AF482715, Orán (Oligoryzomys chacoensis); AF482713, Bermejo (Oligoryzomys occidentalis); AF482714, Lechiguanas (Oligoryzomysflavescens and O. nigripes); AF482711, Buenos Aires (undescribed); AY740624, Araucaria (O. nigripes); KC422346, Juquitiba (O. nigripes); AF291702, AF004660 and AF324902 Andes (Oligoryzomys longicaudatus; HM582091, Jaborá (Akodon montensis) and NC038505 Laguna Negra (Calomys laucha). The model selection retained GTR+G+I as the best model that shows the lowest Akaike (6384.96) and BIC (6645.48) values." ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Isabel E. Gómez Villafañe, Eliana F. Burgos, Rocio M. Coelho, Carla M. Bellomo, Jerónimo Garcilazo Amatti, Valeria P. Martinez" "autores" => array:6 [ 0 => array:2 [ "nombre" => "Isabel E." "apellidos" => "Gómez Villafañe" ] 1 => array:2 [ "nombre" => "Eliana F." "apellidos" => "Burgos" ] 2 => array:2 [ "nombre" => "Rocio M." "apellidos" => "Coelho" ] 3 => array:2 [ "nombre" => "Carla M." "apellidos" => "Bellomo" ] 4 => array:2 [ "nombre" => "Jerónimo" "apellidos" => "Garcilazo Amatti" ] 5 => array:2 [ "nombre" => "Valeria P." "apellidos" => "Martinez" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0325754121000444?idApp=UINPBA00004N" "url" => "/03257541/0000005400000001/v1_202204020607/S0325754121000444/v1_202204020607/en/main.assets" ] "en" => array:19 [ "idiomaDefecto" => true "cabecera" => "Brief report" "titulo" => "Mycoplasma maculosum and Mycoplasma spumans associated with fertility disorders in dogs from a Bernese Mountain dog kennel" "tieneTextoCompleto" => true "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "39" "paginaFinal" => "42" ] ] "autores" => array:1 [ 0 => array:3 [ "autoresLista" => "Pablo Jesús Tamiozzo" "autores" => array:1 [ 0 => array:4 [ "nombre" => "Pablo Jesús" "apellidos" => "Tamiozzo" "email" => array:1 [ 0 => "ptamiozzo@ayv.unrc.edu.ar" ] "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "b" "identificador" => "aff0010" ] ] ] ] "afiliaciones" => array:2 [ 0 => array:3 [ "entidad" => "Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Córdoba, Argentina" "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Laboratorio ACERCA, Jorge Newbery 268, Las Higueras, Córdoba, Argentina" "etiqueta" => "b" "identificador" => "aff0010" ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Mycoplasma maculosum y Mycoplasma spumans asociados con desórdenes de fertilidad en perros de un criadero de boyero de Berna" ] ] "textoCompleto" => "Mollicutes which include the genus Mycoplasma are wall-less bacteria that are found in avian, insect, mammalian and reptilian hosts<a class="elsevierStyleCrossRef" href="#bib0135">11</a>. Until now, more than 15 species have been isolated or detected in dogs<a class="elsevierStyleCrossRef" href="#bib0105">5</a>. In spite of the fact that mycoplasmas can be found in healthy and diseased dogs, some studies have associated the presence of these agents with infertility, respiratory disease, arthritis and colitis<a class="elsevierStyleCrossRef" href="#bib0105">5</a>. Considering that information about the association of canine mycoplasmas with infections in dogs is scarce, this short communication describes a case of fertility disorders and other anomalies attributed to Mycoplasma spumans and Mycoplasma maculosum in a Bernese Mountain dog kennel.The Bernese Mountain dog kennel was located in the city of Mar del Plata (Buenos Aires province). It has been operating normally for 21 years, with high rates of pregnancy, producing two to three litters per year. The kennel was considered to be free of brucellosis and leptospirosis because of the lack of antecedents of these diseases and the negative serology of the dogs, which were tested regularly. Dogs were vaccinated with the sextuple vaccine (polyvalent vaccine that confers immunity against canine distemper virus, canine parvovirus, Leptospira interrogans serovars canicola and icterohaemorrhagiae, canine adenovirus type 2 and canine parainfluenza virus) and dewormed regularly 2–4 times a year. In October 2017 a female (Catalina) and a male (Gucci) dogs were imported from Brazil and incorporated into the breeding stock. These dogs were tested only against Brucella (immunochromatographic rapid test, FASTest® BRUCELLA canis and blood culture), giving negative results. Thereafter, fertility disorders and other anomalies began to be observed: (i) after two unsuccessful matings between a female named Wish and Gucci, a singleton puppy with tracheal aplasia was born, (ii) Gucci's sperm analysis showed low sperm counts (1.5mill/ml), without motility, 100% of dead forms and 70% of abnormalities in head, midpiece and tail, reason enough for being excluded from the kennel, (iii) after a male called Egmont mated Catalina, a puppy died of a fatal pneumonia after an unsuccessful treatment with amoxicillin-clavulanic acid. By June 2019, all the bitches still had pregnancy loses. After having ruled out several infectious agents by bacteriology (Brucella sp., Escherichia coli, Citrobacter freundii and beta-hemolytic streptococci) and serology (microscopic agglutination test for Leptospira sp.) in all the dogs, the owners sent vaginal swabs from three non-pregnant bitches (Catalina, Clara and Troya) for Mycoplasma diagnosis. Once in the laboratory, DNA extraction from vaginal swabs was performed using the Puriprep-S commercial kit (Inbio Highway, Argentina) following the manufacturer's instructions. For Mycoplasma spp. detection, a nested PCR targeting the 16S-23S rRNA intergenic spacer region (ITS) was performed under the conditions reported by the authors<a class="elsevierStyleCrossRef" href="#bib0145">13</a>. Only Catalina rendered a positive result, with two different size bands (330bp and 210bp, approximately) observed in the agarose gel. In order to identify Mycoplasma spp., both PCR products were purified (Puriprep-GP Kit, Inbio Highway), quantified and sequenced (ABI 3130xl; Applied Biosystems) using the inner primers described by Tang et al.<a class="elsevierStyleCrossRef" href="#bib0145">13</a> The sequences were curated using the BioEdit software<a class="elsevierStyleCrossRef" href="#bib0120">8</a> and aligned against the database using nucleotide BLAST (<a href="http://www.ncbi.nlm.nih.gov/blast">http://www.ncbi.nlm.nih.gov/blast</a>). The 16S-23S rRNA ITS sequence obtained from the 310bp amplicon (GenBank accession number MW646397) showed 97.44% similarity with the same region of Mycoplasma maculosum strains NCTC10168 (LR215037.1), Skotti B (FJ595090.1), PG15 (AF443610.1), ATCC 19327 (AY973564.1), 97.12% with Mycoplasma leopharyngis (AY762644.1) and 97.14% with Mycoplasma sp. isolate BA019827 (KX863544.1). The 214bp (GenBank accession number MW646398) sequence showed 98.1% and 97.63 similarity with M. spumans (AF538684.1 and AY762642.1) respectively. Based on these results, in July 2019, the 13 male and female adult dogs from the kennel (even Gucci, the male dog) were treated with doxycycline (500mg/day) for a 15-day period. Two months after the treatment with doxycycline, Gucci's sperm analysis revealed the following parameters: normal sperm counts (52mill/ml), 61% rapid progressive, 19% slow progressive and 12% in situ motility) with 10% of dead forms and 75% of normal forms. Despite the doxycycline treatment, four female dogs (Wish, Catalina, Eureka and Clara) could not get pregnant. For this reason, in August 2019, the owner sent vaginal swabs from two females to the laboratory (Catalina and Eureka). Both samples rendered positive results with one and two bands present in agarose gel, respectively. This time sequencing was not performed. These females were treated with azithromycin (750mg/day) for a nine-day period starting on the day of mating. After that, Gucci mated successfully with Eureka and Troya and, Egmont mated Catalina, the three bitches became pregnant. In December 2019, Troya and Eureka delivered healthy puppies. Catalina could not deliver because during her pregnancy she suffered from a gastric torsion and needed surgery. After this episode, no reproductive disorders were observed. During 2020, 27 healthy puppies were born.The success of the antibiotic treatment used, the absence of other pathogens causing infertility, the detection of Mycoplasmas, the changes in Gucci's sperm analysis and the recovery from the bitches’ previous subfertility after the antibiotic treatments, suggest that Mycoplasma species were associated with that subfertility episode in this dog kennel. Although the results of Gucci's sperm analysis improved after treatment with doxycycline, it was only after the treatment with azithromycin that successful pregnancies were achieved. In this regard, azithromycin, doxycycline, enrofloxacin, marbofloxacin, minocycline, orbifloxacin and pradofloxacin have been pointed out as effective against Mycoplasma-associated respiratory infections in dogs<a class="elsevierStyleCrossRef" href="#bib0125">9</a>. However, azithromycin would be the most suitable antibiotic for the treatment of infections caused by Mycoplasma<a class="elsevierStyleCrossRef" href="#bib0125">9</a>. Although M. canis is the predominant species identified from the vaginas of fertile and infertile bitches<a class="elsevierStyleCrossRefs" href="#bib0110">6,10,12</a> and Mycoplasmacynos had been associated with pneumonia<a class="elsevierStyleCrossRefs" href="#bib0100">4,16</a>, we did not find any of those species. M. spumans and M. maculosum have been identified from the respiratory and genitourinary systems of dogs with and without respiratory infection<a class="elsevierStyleCrossRefs" href="#bib0085">1,2,7</a> and from fertile and infertile dogs<a class="elsevierStyleCrossRefs" href="#bib0090">2,6,10</a>. These antecedents might explain the subfertility episode in this kennel, the fatal pneumonia of Catalina's puppy and even, the tracheal aplasia of Wish's puppy. Further experimental studies are necessary to confirm our results. With regard to M. leopharyngis, we did not find any antecedents about its presence in dogs, since together with other six Mycoplasma species, it affects felines<a class="elsevierStyleCrossRef" href="#bib0095">3</a>. Considering the high similarity (98.6%) in the 16S-23S rRNA ITS region between M. maculosum and M. leopharyngis<a class="elsevierStyleCrossRef" href="#bib0150">14</a>, the detected species could be M. maculosum. However, the presence of M. leopharyngis in the urogenital tract of dogs cannot be strictly ruled out. The analysis of other target sequences would have allowed the unambiguous identification of Mycoplasma species. Taking into account the phylogenetic relatedness and pairwise sequence similarities of the species within the family Mycoplasmataceae, a reliable and useful taxonomic tool, analyzing 16S-23S rRNA ITS, RNA polymerase beta subunit (rpoB) and 16S rRNA genes, has been proposed<a class="elsevierStyleCrossRef" href="#bib0155">15</a>. Regrettably, rpoB and 16S rRNA genes were not addressed in this study. In this way, the analysis of the three-target sequence has been pointed out as a valuable tool for routine analyses<a class="elsevierStyleCrossRef" href="#bib0155">15</a>.Unfortunately, veterinarians do not often consider Mycoplasma in the differential diagnosis of respiratory and reproductive diseases in dogs. For this reason, Mycoplasma detection either by culture or molecular-based tests is rarely requested. From a practical point of view, the detection of these agents is important for the implementation of an adequate antibiotic treatment, beyond the identification of the involved species. Due to the lack of cell wall in Mycoplasma, they are inherently resistant to β-lactam antibiotics. In this report, prolonged therapy with doxycycline and azithromycin demonstrated to be effective against Mycoplasma infections, agreeing with previous data<a class="elsevierStyleCrossRef" href="#bib0125">9</a>. In spite of the limitation of this study, since Mycoplasma culture was not tried, the importance of the diagnosis of these agents was demonstrated.Conflict of interestThe author declares that he has no conflicts of interest." "textoCompletoSecciones" => array:1 [ "secciones" => array:8 [ 0 => array:3 [ "identificador" => "xres1688163" "titulo" => "Highlights" "secciones" => array:1 [ 0 => array:1 [ "identificador" => "abst0005" ] ] ] 1 => array:3 [ "identificador" => "xres1688164" "titulo" => "Abstract" "secciones" => array:1 [ 0 => array:1 [ "identificador" => "abst0010" ] ] ] 2 => array:2 [ "identificador" => "xpalclavsec1497090" "titulo" => "Keywords" ] 3 => array:3 [ "identificador" => "xres1688162" "titulo" => "Resumen" "secciones" => array:1 [ 0 => array:1 [ "identificador" => "abst0015" ] ] ] 4 => array:2 [ "identificador" => "xpalclavsec1497091" "titulo" => "Palabras clave" ] 5 => array:2 [ "identificador" => "sec0005" "titulo" => "Conflict of interest" ] 6 => array:2 [ "identificador" => "xack595658" "titulo" => "Acknowledgements" ] 7 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2020-09-16" "fechaAceptado" => "2021-04-12" "PalabrasClave" => array:2 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec1497090" "palabras" => array:6 [ 0 => "Dogs" 1 => "Fertility disorders" 2 => "Urogenital tract" 3 => "Mycoplasma maculosum" 4 => "Mycoplasma spumans" 5 => "Antibiotic treatment" ] ] ] "es" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec1497091" "palabras" => array:6 [ 0 => "Perros" 1 => "Trastornos de fertilidad" 2 => "Tracto urogenital" 3 => "Mycoplasma maculosum" 4 => "Mycoplasma spumans" 5 => "Tratamiento antibiótico" ] ] ] ] "tieneResumen" => true "highlights" => array:2 [ "titulo" => "Highlights" "resumen" => "<ul class="elsevierStyleList" id="lis0005"><li class="elsevierStyleListItem" id="lsti0005">•Mycoplasma maculosum and Mycoplasma spumans were detected by PCR in dogs.</li><li class="elsevierStyleListItem" id="lsti0010">•After antibiotic treatment reproductive disorders disappeared.</li><li class="elsevierStyleListItem" id="lsti0015">•Veterinarians not often consider Mycoplasma in the differential diagnosis of reproductive diseases.</li></ul>" ] "resumen" => array:2 [ "en" => array:2 [ "titulo" => "Abstract" "resumen" => "The aim of this short communication is to describe a case of subfertility and other anomalies associated with the presence of Mycoplasma spumans and Mycoplasma maculosum in a Bernese Mountain Dog kennel. After the arrival of two dogs from abroad, some fertility disorders, such as unsuccessful mating, pregnancy losses and abnormal sperm analysis results, were observed. Two consecutive samplings (vaginal swabs) of three and two bitches with problems, respectively, were performed and M. spumans and M. maculosum were identified by PCR and sequencing. After treatment for 15 days with doxycycline and 9 days with azithromycin, successful pregnancies were achieved and the results of the sperm analyses were reversed. Considering that no other infectious agents causing subfertility problems were detected and that no management measures or other medication apart from these antibiotics were applied, it was concluded that fertility problems were due to the presence of these two Mycoplasma species." ] "es" => array:2 [ "titulo" => "Resumen" "resumen" => "El objetivo de esta comunicación es describir un caso de subfertilidad y otras anomalías asociadas a la presencia de Mycoplasma spumans y Mycoplasma maculosum en un criadero de perros boyero de Berna. Después de la entrada de 2 perros del exterior, comenzaron a observarse algunos trastornos de la fertilidad, como apareamientos infructuosos, pérdidas de preñez y resultados anormales en el análisis espermático. Se realizaron 2 muestreos consecutivos (hisopados vaginales) de 3 y 2 perras con problemas, respectivamente, y se identificó M. spumans y M. maculosum mediante PCR y secuenciación. Después del tratamiento con doxiciclina y azitromicina durante 15 y 9 días, respectivamente, se lograron obtener preñeces exitosas y se revirtieron los resultados del espermograma. Considerando que no se detectaron otros agentes infecciosos causantes de problemas de subfertilidad y que no se aplicó ninguna medida de manejo ni otra medicación, aparte de los tratamientos antibióticos mencionados, se concluyó que los problemas de fertilidad se debieron a la presencia de estas 2 especies de Mycoplasma." ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0015" "bibliografiaReferencia" => array:16 [ 0 => array:3 [ "identificador" => "bib0085" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Previously uncharacterized Mycoplasma isolates from an investigation of canine pneumonia" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "D. Armstrong" 1 => "J.G. Tully" 2 => "B. Yu" 3 => "V. Morton" 4 => "M.H. Friedman" 5 => "L. Steger" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Infect Immun" "fecha" => "1970" "volumen" => "1" "paginaInicial" => "1" "paginaFinal" => "7" ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib0090" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Isolation and rapid identification of Mycoplasma species from canine tissues by plate immunofluorescence" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "M.F. Barile" 1 => "R.A. Del Giudice" 2 => "T.R. Carski" 3 => "H.M. Yamashiroya" 4 => "J.A. Verna" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.3181/00379727-134-34747" "Revista" => array:6 [ "tituloSerie" => "Proc Soc Exp Biol Med" "fecha" => "1970" "volumen" => "134" "paginaInicial" => "146" "paginaFinal" => "148" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/4912557" "web" => "Medline" ] ] ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bib0095" "etiqueta" => "3" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Taxonomy of the feline mycoplasmas Mycoplasma felifaucium, Mycoplasma feliminutum, Mycoplasma felis, Mycoplasma gateae, Mycoplasma leocaptivus, Mycoplasma leopharyngis, and Mycoplasma simbae by 16S rRNA gene sequence comparisons" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "D.R. Brown" 1 => "G.S. McLaughlin" 2 => "M.B. Brown" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1099/00207713-45-3-560" "Revista" => array:6 [ "tituloSerie" => "Int J Syst Bacteriol" "fecha" => "1995" "volumen" => "45" "paginaInicial" => "560" "paginaFinal" => "564" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8590684" "web" => "Medline" ] ] ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bib0100" "etiqueta" => "4" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Mycoplasmas associated with canine infectious respiratory disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:7 [ 0 => "V.J. Chalker" 1 => "W.M. Owen" 2 => "C. Paterson" 3 => "E. Barker" 4 => "H. Brooks" 5 => "A.N. Rycroft" 6 => "J. Brownlie" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1099/mic.0.26848-0" "Revista" => array:6 [ "tituloSerie" => "Microbiology" "fecha" => "2004" "volumen" => "150" "paginaInicial" => "3491" "paginaFinal" => "3497" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15470126" "web" => "Medline" ] ] ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bib0105" "etiqueta" => "5" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Canine mycoplasmas" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "V.J. Chalker" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.rvsc.2004.10.002" "Revista" => array:6 [ "tituloSerie" => "Res Vet Sci" "fecha" => "2005" "volumen" => "79" "paginaInicial" => "1" "paginaFinal" => "8" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15894017" "web" => "Medline" ] ] ] ] ] ] ] ] 5 => array:3 [ "identificador" => "bib0110" "etiqueta" => "6" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The genital Mycoplasma and Ureaplasma flora of healthy and diseased dogs" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "P.A. Doig" 1 => "H.L. Ruhnke" 2 => "W.T. Bosu" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Can J Comp Med" "fecha" => "1981" "volumen" => "45" "paginaInicial" => "233" "paginaFinal" => "328" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7340908" "web" => "Medline" ] ] ] ] ] ] ] ] 6 => array:3 [ "identificador" => "bib0115" "etiqueta" => "7" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The isolation of organisms of the pleuropneumonia group from dogs" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "D.G. Edward" 1 => "W.A. Fitzgerald" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1099/00221287-5-3-566" "Revista" => array:6 [ "tituloSerie" => "J Gen Microbiol" "fecha" => "1951" "volumen" => "5" "paginaInicial" => "566" "paginaFinal" => "575" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/14873903" "web" => "Medline" ] ] ] ] ] ] ] ] 7 => array:3 [ "identificador" => "bib0120" "etiqueta" => "8" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "T.A. Hall" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Nucleic Acids Symp Ser" "fecha" => "1999" "volumen" => "41" "paginaInicial" => "95" "paginaFinal" => "98" ] ] ] ] ] ] 8 => array:3 [ "identificador" => "bib0125" "etiqueta" => "9" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Antimicrobial use guidelines for treatment of respiratory tract disease in dogs and cats: antimicrobial guidelines working group of the International Society for Companion Animal Infectious Diseases" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:11 [ 0 => "M.R. Lappin" 1 => "J. Blondeau" 2 => "D. Boothe" 3 => "E.B. Breitschwerdt" 4 => "L. Guardabassi" 5 => "D.H. Lloyd" 6 => "M.G. Papich" 7 => "S.C. Rankin" 8 => "J.E. Sykes" 9 => "J. Turnidge" 10 => "J.S. Weese" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/jvim.14627" "Revista" => array:6 [ "tituloSerie" => "J Vet Intern Med" "fecha" => "2017" "volumen" => "31" "paginaInicial" => "279" "paginaFinal" => "284" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/28185306" "web" => "Medline" ] ] ] ] ] ] ] ] 9 => array:3 [ "identificador" => "bib0130" "etiqueta" => "10" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Genital mycoplasmas of healthy bitches" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "Z. Maksimović" 1 => "A. Maksimović" 2 => "A. Halilbašić" 3 => "M. Rifatbegović" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1177/1040638718778745" "Revista" => array:6 [ "tituloSerie" => "J Vet Diagn Invest" "fecha" => "2018" "volumen" => "30" "paginaInicial" => "651" "paginaFinal" => "653" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/29790451" "web" => "Medline" ] ] ] ] ] ] ] ] 10 => array:3 [ "identificador" => "bib0135" "etiqueta" => "11" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Molecular biology and pathogenicity of mycoplasmas" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "S. Razin" 1 => "D. Yogev" 2 => "Y. Naot" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1128/MMBR.62.4.1094-1156.1998" "Revista" => array:6 [ "tituloSerie" => "Microbiol Mol Biol Rev" "fecha" => "1998" "volumen" => "62" "paginaInicial" => "1094" "paginaFinal" => "1156" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9841667" "web" => "Medline" ] ] ] ] ] ] ] ] 11 => array:3 [ "identificador" => "bib0140" "etiqueta" => "12" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Identification and differentiation of canine Mycoplasma isolates by 16S-23S rDNA PCR-RFLP" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "J. Spergser" 1 => "R. Rosengarten" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.vetmic.2007.04.045" "Revista" => array:6 [ "tituloSerie" => "Vet Microbiol" "fecha" => "2007" "volumen" => "125" "paginaInicial" => "170" "paginaFinal" => "174" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17544231" "web" => "Medline" ] ] ] ] ] ] ] ] 12 => array:3 [ "identificador" => "bib0145" "etiqueta" => "13" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "A polymerase chain reaction-based method for detecting Mycoplasma/Acholeplasma contaminants in cell culture" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "J. Tang" 1 => "M. Hu" 2 => "S. Lee" 3 => "R. Roblin" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/s0167-7012(99)00107-4" "Revista" => array:6 [ "tituloSerie" => "J Microbiol Methods" "fecha" => "2000" "volumen" => "39" "paginaInicial" => "121" "paginaFinal" => "126" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10576701" "web" => "Medline" ] ] ] ] ] ] ] ] 13 => array:3 [ "identificador" => "bib0150" "etiqueta" => "14" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Sequencing of the intergenic 16S-23S rRNA spacer (ITS) region of Mollicutes species and their identification using microarray-based assay and DNA sequencing" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "D.V. Volokhov" 1 => "J. George" 2 => "S.X. Liu" 3 => "P. Ikonomi" 4 => "C. Anderson" 5 => "V. Chizhikov" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1007/s00253-005-0280-7" "Revista" => array:6 [ "tituloSerie" => "Appl Microbiol Biotechnol" "fecha" => "2006" "volumen" => "71" "paginaInicial" => "680" "paginaFinal" => "698" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16470366" "web" => "Medline" ] ] ] ] ] ] ] ] 14 => array:3 [ "identificador" => "bib0155" "etiqueta" => "15" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "RNA polymerase beta subunit (rpoB) gene and the 16S-23S rRNA intergenic transcribed spacer region (ITS) as complementary molecular markers in addition to the 16S rRNA gene for phylogenetic analysis and identification of the species of the family Mycoplasmataceae" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "D.V. Volokhov" 1 => "V. Simonyan" 2 => "M.K. Davidson" 3 => "V.E. Chizhikov" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.ympev.2011.11.002" "Revista" => array:6 [ "tituloSerie" => "Mol Phylogenet Evol" "fecha" => "2012" "volumen" => "62" "paginaInicial" => "515" "paginaFinal" => "528" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22115576" "web" => "Medline" ] ] ] ] ] ] ] ] 15 => array:3 [ "identificador" => "bib0160" "etiqueta" => "16" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Lethal bronchopneumonia caused by Mycoplasma cynos in a litter of golden retriever puppies" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "F. Zeugswetter" 1 => "H. Weissenböck" 2 => "S. Shibly" 3 => "J. Hassan" 4 => "J. Spergser" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1136/vr.161.18.626" "Revista" => array:6 [ "tituloSerie" => "Vet Rec" "fecha" => "2007" "volumen" => "161" "paginaInicial" => "626" "paginaFinal" => "627" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17982143" "web" => "Medline" ] ] ] ] ] ] ] ] ] ] ] ] "agradecimientos" => array:1 [ 0 => array:4 [ "identificador" => "xack595658" "titulo" => "Acknowledgements" "texto" => "This study was financially supported in part by FONCyT-ANPCyT-MinCyT, República Argentina [PICT 02148/2018], PPI 2019-2021 SeCyT-UNRC and IDi division of ACERCA Laboratorio. The author is deeply grateful to the owners of the Bernese Mountain dog kennel, Silvina Otero and Gustavo Pisani and to the veterinarians Ricardo Abaurrea, Carlos Enrique Sorribas and Isabel Zubiros." "vista" => "all" ] ] ] "idiomaDefecto" => "en" "url" => "/03257541/0000005400000001/v1_202204020607/S0325754121000523/v1_202204020607/en/main.assets" "Apartado" => array:4 [ "identificador" => "37861" "tipo" => "SECCION" "en" => array:2 [ "titulo" => "Microbiología clínica y enfermedades infecciosas" "idiomaDefecto" => true ] "idiomaDefecto" => "en" ] "PDF" => "https://static.elsevier.es/multimedia/03257541/0000005400000001/v1_202204020607/S0325754121000523/v1_202204020607/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0325754121000523?idApp=UINPBA00004N"]

Statistics

Brief report

Mycoplasma maculosum y Mycoplasma spumans asociados con desórdenes de fertilidad en perros de un criadero de boyero de Berna

Pablo Jesús Tamiozzoa,b

a Departamento de Patología Animal, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Córdoba, Argentina

b Laboratorio ACERCA, Jorge Newbery 268, Las Higueras, Córdoba, Argentina

Read

2267

Times
was read the article

987

Total PDF

1280

Total HTML

Share statistics

 array:24 [
  "pii" => "S0325754121000523"
  "issn" => "03257541"
  "doi" => "10.1016/j.ram.2021.04.001"
  "estado" => "S300"
  "fechaPublicacion" => "2022-01-01"
  "aid" => "462"
  "copyright" => "Asociaci&#243;n Argentina de Microbiolog&#237;a"
  "copyrightAnyo" => "2021"
  "documento" => "article"
  "crossmark" => 1
  "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
  "subdocumento" => "fla"
  "cita" => "Rev Argent Microbiol. 2022;54:39-42"
  "abierto" => array:3 [
    "ES" => true
    "ES2" => true
    "LATM" => true
  ]
  "gratuito" => true
  "lecturas" => array:1 [
    "total" => 0
  ]
  "itemSiguiente" => array:19 [
    "pii" => "S0325754121000468"
    "issn" => "03257541"
    "doi" => "10.1016/j.ram.2021.02.011"
    "estado" => "S300"
    "fechaPublicacion" => "2022-01-01"
    "aid" => "456"
    "copyright" => "Asociaci&#243;n Argentina de Microbiolog&#237;a"
    "documento" => "article"
    "crossmark" => 1
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "fla"
    "cita" => "Rev Argent Microbiol. 2022;54:43-7"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:1 [
      "total" => 0
    ]
    "en" => array:14 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Brief report</span>"
      "titulo" => "Clinical and epidemiological features of tuberculosis isolated from critically ill patients"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => array:3 [
        0 => "en"
        1 => "en"
        2 => "es"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "43"
          "paginaFinal" => "47"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "es" => array:1 [
          "titulo" => "Tuberculosis en la unidad de cuidados intensivos&#58; caracter&#237;sticas cl&#237;nicas y epidemiol&#243;gicas"
        ]
      ]
      "contieneResumen" => array:2 [
        "en" => true
        "es" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "resumenGrafico" => array:2 [
        "original" => 0
        "multimedia" => array:7 [
          "identificador" => "fig0005"
          "etiqueta" => "Figure 1"
          "tipo" => "MULTIMEDIAFIGURA"
          "mostrarFloat" => true
          "mostrarDisplay" => false
          "figura" => array:1 [
            0 => array:4 [
              "imagen" => "gr1.jpeg"
              "Alto" => 1804
              "Ancho" => 3175
              "Tamanyo" => 299112
            ]
          ]
          "descripcion" => array:1 [
            "en" => "<p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Minimum spanning tree cluster analysis&#46; &#40;A&#41; Samples in red correspond to ICU samples&#59; samples in blue correspond to community samples&#46; Samples of both groups were present in all clusters&#46; &#40;B&#41; Same analysis colored by lineage&#46; LAM and Haarlem lineages correspond to the most frequent isolates&#46;</p>"
          ]
        ]
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Joaqu&#237;n Hurtado, Cecilia Coitinho, Nicol&#225;s Nin, Mar&#237;a Buroni, Francisco Javier Hurtado, Carlos Robello, Gonzalo Greif"
          "autores" => array:7 [
            0 => array:2 [
              "nombre" => "Joaqu&#237;n"
              "apellidos" => "Hurtado"
            ]
            1 => array:2 [
              "nombre" => "Cecilia"
              "apellidos" => "Coitinho"
            ]
            2 => array:2 [
              "nombre" => "Nicol&#225;s"
              "apellidos" => "Nin"
            ]
            3 => array:2 [
              "nombre" => "Mar&#237;a"
              "apellidos" => "Buroni"
            ]
            4 => array:2 [
              "nombre" => "Francisco Javier"
              "apellidos" => "Hurtado"
            ]
            5 => array:2 [
              "nombre" => "Carlos"
              "apellidos" => "Robello"
            ]
            6 => array:2 [
              "nombre" => "Gonzalo"
              "apellidos" => "Greif"
            ]
          ]
        ]
      ]
      "resumen" => array:1 [
        0 => array:3 [
          "titulo" => "Highlights"
          "clase" => "author-highlights"
          "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall"><ul class="elsevierStyleList" id="lis0005"><li class="elsevierStyleListItem" id="lsti0005"><span class="elsevierStyleLabel">&#8226;</span><p id="par0005" class="elsevierStylePara elsevierViewall">Genetic structure analysis of 60 TB strains isolated from patients from an ICU&#46;</p></li><li class="elsevierStyleListItem" id="lsti0010"><span class="elsevierStyleLabel">&#8226;</span><p id="par0010" class="elsevierStylePara elsevierViewall">We present a comparison with population circulating strains&#46;</p></li><li class="elsevierStyleListItem" id="lsti0015"><span class="elsevierStyleLabel">&#8226;</span><p id="par0015" class="elsevierStylePara elsevierViewall">We evaluate the correlation between the clinical outcome and genotypic data&#46;</p></li><li class="elsevierStyleListItem" id="lsti0020"><span class="elsevierStyleLabel">&#8226;</span><p id="par0020" class="elsevierStylePara elsevierViewall">We present an extensive cohort study done in critically ill patients with tuberculosis&#46;</p></li></ul></p></span>"
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0325754121000468?idApp=UINPBA00004N"
    "url" => "/03257541/0000005400000001/v1_202204020607/S0325754121000468/v1_202204020607/en/main.assets"
  ]
  "itemAnterior" => array:19 [
    "pii" => "S0325754121000444"
    "issn" => "03257541"
    "doi" => "10.1016/j.ram.2021.02.009"
    "estado" => "S300"
    "fechaPublicacion" => "2022-01-01"
    "aid" => "454"
    "copyright" => "Asociaci&#243;n Argentina de Microbiolog&#237;a"
    "documento" => "article"
    "crossmark" => 1
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "fla"
    "cita" => "Rev Argent Microbiol. 2022;54:35-8"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:1 [
      "total" => 0
    ]
    "en" => array:13 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Brief report</span>"
      "titulo" => "Expanded distribution of orthohantavirus Pergamino genotype&#58; First record in Entre Rios province&#44; Argentina"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => array:2 [
        0 => "en"
        1 => "es"
      ]
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "35"
          "paginaFinal" => "38"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "es" => array:1 [
          "titulo" => "Ampliaci&#243;n de la distribuci&#243;n del <span class="elsevierStyleItalic">Orthohantavirus</span> genotipo Pergamino&#58; Primer registro en la Provincia de Entre R&#237;os&#44; Argentina"
        ]
      ]
      "contieneResumen" => array:2 [
        "en" => true
        "es" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "resumenGrafico" => array:2 [
        "original" => 0
        "multimedia" => array:7 [
          "identificador" => "fig0005"
          "etiqueta" => "Figure 1"
          "tipo" => "MULTIMEDIAFIGURA"
          "mostrarFloat" => true
          "mostrarDisplay" => false
          "figura" => array:1 [
            0 => array:4 [
              "imagen" => "gr1.jpeg"
              "Alto" => 1120
              "Ancho" => 2341
              "Tamanyo" => 162142
            ]
          ]
          "descripcion" => array:1 [
            "en" => "<p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Molecular phylogenetic analysis by Maximum Likelihood method&#46; Phylogenetic analysis of hantaviruses based on 853<span class="elsevierStyleHsp" style=""></span>bp of S-segment genome of Andes virus&#44; and other orthohantaviruses characterized previously&#46; Taxa in the branches of the tree indicate virus name and province of origin&#46; GenBank accession numbers and reservoir rodent specie were&#58; <span class="elsevierStyleInterRef" id="intr0005" href="ncbi-n:AF482717">AF482717</span>&#44; Pergamino &#40;<span class="elsevierStyleItalic">Akodon azarae</span>&#41;&#59; <span class="elsevierStyleInterRef" id="intr0010" href="ncbi-n:AF482716">AF482716</span>&#44; Maciel &#40;<span class="elsevierStyleItalic">Necromys benefactus</span>&#41;&#59; <span class="elsevierStyleInterRef" id="intr0015" href="ncbi-n:AF482715">AF482715</span>&#44; Or&#225;n &#40;<span class="elsevierStyleItalic">Oligoryzomys chacoensis</span>&#41;&#59; <span class="elsevierStyleInterRef" id="intr0020" href="ncbi-n:AF482713">AF482713</span>&#44; Bermejo &#40;<span class="elsevierStyleItalic">Oligoryzomys occidentalis</span>&#41;&#59; <span class="elsevierStyleInterRef" id="intr0025" href="ncbi-n:AF482714">AF482714</span>&#44; Lechiguanas &#40;<span class="elsevierStyleItalic">Oligoryzomys</span><span class="elsevierStyleItalic">flavesc</span>ens and <span class="elsevierStyleItalic">O&#46; nigripes</span>&#41;&#59; <span class="elsevierStyleInterRef" id="intr0030" href="ncbi-n:AF482711">AF482711</span>&#44; Buenos Aires &#40;undescribed&#41;&#59; <span class="elsevierStyleInterRef" id="intr0035" href="ncbi-n:AY740624">AY740624</span>&#44; Araucaria &#40;<span class="elsevierStyleItalic">O&#46; nigripes</span>&#41;&#59; <span class="elsevierStyleInterRef" id="intr0040" href="ncbi-n:KC422346">KC422346</span>&#44; Juquitiba &#40;<span class="elsevierStyleItalic">O&#46; nigripes</span>&#41;&#59; <span class="elsevierStyleInterRef" id="intr0045" href="ncbi-n:AF291702">AF291702</span>&#44; <span class="elsevierStyleInterRef" id="intr0050" href="ncbi-n:AF004660">AF004660</span> and <span class="elsevierStyleInterRef" id="intr0055" href="ncbi-n:AF324902">AF324902</span> Andes &#40;<span class="elsevierStyleItalic">Oligoryzomys longicaudatus</span>&#59; <span class="elsevierStyleInterRef" id="intr0060" href="ncbi-n:HM582091">HM582091</span>&#44; Jabor&#225; &#40;<span class="elsevierStyleItalic">Akodon montensis</span>&#41; and NC038505 Laguna Negra &#40;<span class="elsevierStyleItalic">Calomys laucha</span>&#41;&#46; The model selection retained GTR&#43;G&#43;I as the best model that shows the lowest Akaike &#40;6384&#46;96&#41; and BIC &#40;6645&#46;48&#41; values&#46;</p>"
          ]
        ]
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Isabel E&#46; G&#243;mez Villafa&#241;e, Eliana F&#46; Burgos, Rocio M&#46; Coelho, Carla M&#46; Bellomo, Jer&#243;nimo Garcilazo Amatti, Valeria P&#46; Martinez"
          "autores" => array:6 [
            0 => array:2 [
              "nombre" => "Isabel E&#46;"
              "apellidos" => "G&#243;mez Villafa&#241;e"
            ]
            1 => array:2 [
              "nombre" => "Eliana F&#46;"
              "apellidos" => "Burgos"
            ]
            2 => array:2 [
              "nombre" => "Rocio M&#46;"
              "apellidos" => "Coelho"
            ]
            3 => array:2 [
              "nombre" => "Carla M&#46;"
              "apellidos" => "Bellomo"
            ]
            4 => array:2 [
              "nombre" => "Jer&#243;nimo"
              "apellidos" => "Garcilazo Amatti"
            ]
            5 => array:2 [
              "nombre" => "Valeria P&#46;"
              "apellidos" => "Martinez"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0325754121000444?idApp=UINPBA00004N"
    "url" => "/03257541/0000005400000001/v1_202204020607/S0325754121000444/v1_202204020607/en/main.assets"
  ]
  "en" => array:19 [
    "idiomaDefecto" => true
    "cabecera" => "<span class="elsevierStyleTextfn">Brief report</span>"
    "titulo" => "<span class="elsevierStyleItalic">Mycoplasma maculosum</span> and <span class="elsevierStyleItalic">Mycoplasma spumans</span> associated with fertility disorders in dogs from a Bernese Mountain dog kennel"
    "tieneTextoCompleto" => true
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "39"
        "paginaFinal" => "42"
      ]
    ]
    "autores" => array:1 [
      0 => array:3 [
        "autoresLista" => "Pablo Jes&#250;s Tamiozzo"
        "autores" => array:1 [
          0 => array:4 [
            "nombre" => "Pablo Jes&#250;s"
            "apellidos" => "Tamiozzo"
            "email" => array:1 [
              0 => "ptamiozzo@ayv.unrc.edu.ar"
            ]
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff0010"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:2 [
          0 => array:3 [
            "entidad" => "Departamento de Patolog&#237;a Animal&#44; Facultad de Agronom&#237;a y Veterinaria&#44; Universidad Nacional de R&#237;o Cuarto&#44; C&#243;rdoba&#44; Argentina"
            "etiqueta" => "a"
            "identificador" => "aff0005"
          ]
          1 => array:3 [
            "entidad" => "Laboratorio ACERCA&#44; Jorge Newbery 268&#44; Las Higueras&#44; C&#243;rdoba&#44; Argentina"
            "etiqueta" => "b"
            "identificador" => "aff0010"
          ]
        ]
      ]
    ]
    "titulosAlternativos" => array:1 [
      "es" => array:1 [
        "titulo" => "<span class="elsevierStyleItalic">Mycoplasma maculosum</span> y <span class="elsevierStyleItalic">Mycoplasma spumans</span> asociados con des&#243;rdenes de fertilidad en perros de un criadero de boyero de Berna"
      ]
    ]
    "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0020" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Mollicutes</span> which include the genus <span class="elsevierStyleItalic">Mycoplasma</span> are wall-less bacteria that are found in avian&#44; insect&#44; mammalian and reptilian hosts<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">11</span></a>&#46; Until now&#44; more than 15 species have been isolated or detected in dogs<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">5</span></a>&#46; In spite of the fact that <span class="elsevierStyleItalic">mycoplasmas</span> can be found in healthy and diseased dogs&#44; some studies have associated the presence of these agents with infertility&#44; respiratory disease&#44; arthritis and colitis<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">5</span></a>&#46; Considering that information about the association of canine <span class="elsevierStyleItalic">mycoplasmas</span> with infections in dogs is scarce&#44; this short communication describes a case of fertility disorders and other anomalies attributed to <span class="elsevierStyleItalic">Mycoplasma spumans</span> and <span class="elsevierStyleItalic">Mycoplasma maculosum</span> in a Bernese Mountain dog kennel&#46;</p><p id="par0025" class="elsevierStylePara elsevierViewall">The Bernese Mountain dog kennel was located in the city of Mar del Plata &#40;Buenos Aires province&#41;&#46; It has been operating normally for 21 years&#44; with high rates of pregnancy&#44; producing two to three litters per year&#46; The kennel was considered to be free of brucellosis and leptospirosis because of the lack of antecedents of these diseases and the negative serology of the dogs&#44; which were tested regularly&#46; Dogs were vaccinated with the sextuple vaccine &#40;polyvalent vaccine that confers immunity against canine distemper virus&#44; canine parvovirus&#44; <span class="elsevierStyleItalic">Leptospira interrogans</span> serovars <span class="elsevierStyleItalic">canicola</span> and <span class="elsevierStyleItalic">icterohaemorrhagiae</span>&#44; canine adenovirus type 2 and canine parainfluenza virus&#41; and dewormed regularly 2&#8211;4 times a year&#46; In October 2017 a female &#40;Catalina&#41; and a male &#40;Gucci&#41; dogs were imported from Brazil and incorporated into the breeding stock&#46; These dogs were tested only against <span class="elsevierStyleItalic">Brucella</span> &#40;immunochromatographic rapid test&#44; FASTest&#174; BRUCELLA canis and blood culture&#41;&#44; giving negative results&#46; Thereafter&#44; fertility disorders and other anomalies began to be observed&#58; &#40;i&#41; after two unsuccessful matings between a female named Wish and Gucci&#44; a singleton puppy with tracheal aplasia was born&#44; &#40;ii&#41; Gucci&#39;s sperm analysis showed low sperm counts &#40;1&#46;5<span class="elsevierStyleHsp" style=""></span>mill&#47;ml&#41;&#44; without motility&#44; 100&#37; of dead forms and 70&#37; of abnormalities in head&#44; midpiece and tail&#44; reason enough for being excluded from the kennel&#44; &#40;iii&#41; after a male called Egmont mated Catalina&#44; a puppy died of a fatal pneumonia after an unsuccessful treatment with amoxicillin-clavulanic acid&#46; By June 2019&#44; all the bitches still had pregnancy loses&#46; After having ruled out several infectious agents by bacteriology &#40;<span class="elsevierStyleItalic">Brucella</span> sp&#46;&#44; <span class="elsevierStyleItalic">Escherichia coli</span>&#44; <span class="elsevierStyleItalic">Citrobacter freundii</span> and beta-hemolytic streptococci&#41; and serology &#40;microscopic agglutination test for <span class="elsevierStyleItalic">Leptospira</span> sp&#46;&#41; in all the dogs&#44; the owners sent vaginal swabs from three non-pregnant bitches &#40;Catalina&#44; Clara and Troya&#41; for <span class="elsevierStyleItalic">Mycoplasma</span> diagnosis&#46; Once in the laboratory&#44; DNA extraction from vaginal swabs was performed using the Puriprep-S commercial kit &#40;Inbio Highway&#44; Argentina&#41; following the manufacturer&#39;s instructions&#46; For <span class="elsevierStyleItalic">Mycoplasma</span> spp&#46; detection&#44; a nested PCR targeting the 16S-23S rRNA intergenic spacer region &#40;ITS&#41; was performed under the conditions reported by the authors<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">13</span></a>&#46; Only Catalina rendered a positive result&#44; with two different size bands &#40;330<span class="elsevierStyleHsp" style=""></span>bp and 210<span class="elsevierStyleHsp" style=""></span>bp&#44; approximately&#41; observed in the agarose gel&#46; In order to identify <span class="elsevierStyleItalic">Mycoplasma</span> spp&#46;&#44; both PCR products were purified &#40;Puriprep-GP Kit&#44; Inbio Highway&#41;&#44; quantified and sequenced &#40;ABI 3130xl&#59; Applied Biosystems&#41; using the inner primers described by Tang et al&#46;<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">13</span></a> The sequences were curated using the BioEdit software<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">8</span></a> and aligned against the database using nucleotide BLAST &#40;<a href="http://www.ncbi.nlm.nih.gov/blast">http&#58;&#47;&#47;www&#46;ncbi&#46;nlm&#46;nih&#46;gov&#47;blast</a>&#41;&#46; The 16S-23S rRNA ITS sequence obtained from the 310<span class="elsevierStyleHsp" style=""></span>bp amplicon &#40;GenBank accession number MW646397&#41; showed 97&#46;44&#37; similarity with the same region of <span class="elsevierStyleItalic">Mycoplasma maculosum</span> strains NCTC10168 &#40;LR215037&#46;1&#41;&#44; Skotti B &#40;FJ595090&#46;1&#41;&#44; PG15 &#40;AF443610&#46;1&#41;&#44; ATCC 19327 &#40;AY973564&#46;1&#41;&#44; 97&#46;12&#37; with <span class="elsevierStyleItalic">Mycoplasma leopharyngis</span> &#40;AY762644&#46;1&#41; and 97&#46;14&#37; with <span class="elsevierStyleItalic">Mycoplasma</span> sp&#46; isolate BA019827 &#40;KX863544&#46;1&#41;&#46; The 214<span class="elsevierStyleHsp" style=""></span>bp &#40;GenBank accession number MW646398&#41; sequence showed 98&#46;1&#37; and 97&#46;63 similarity with <span class="elsevierStyleItalic">M&#46; spumans</span> &#40;AF538684&#46;1 and AY762642&#46;1&#41; respectively&#46; Based on these results&#44; in July 2019&#44; the 13 male and female adult dogs from the kennel &#40;even Gucci&#44; the male dog&#41; were treated with doxycycline &#40;500<span class="elsevierStyleHsp" style=""></span>mg&#47;day&#41; for a 15-day period&#46; Two months after the treatment with doxycycline&#44; Gucci&#39;s sperm analysis revealed the following parameters&#58; normal sperm counts &#40;52<span class="elsevierStyleHsp" style=""></span>mill&#47;ml&#41;&#44; 61&#37; rapid progressive&#44; 19&#37; slow progressive and 12&#37; <span class="elsevierStyleItalic">in situ</span> motility&#41; with 10&#37; of dead forms and 75&#37; of normal forms&#46; Despite the doxycycline treatment&#44; four female dogs &#40;Wish&#44; Catalina&#44; Eureka and Clara&#41; could not get pregnant&#46; For this reason&#44; in August 2019&#44; the owner sent vaginal swabs from two females to the laboratory &#40;Catalina and Eureka&#41;&#46; Both samples rendered positive results with one and two bands present in agarose gel&#44; respectively&#46; This time sequencing was not performed&#46; These females were treated with azithromycin &#40;750<span class="elsevierStyleHsp" style=""></span>mg&#47;day&#41; for a nine-day period starting on the day of mating&#46; After that&#44; Gucci mated successfully with Eureka and Troya and&#44; Egmont mated Catalina&#44; the three bitches became pregnant&#46; In December 2019&#44; Troya and Eureka delivered healthy puppies&#46; Catalina could not deliver because during her pregnancy she suffered from a gastric torsion and needed surgery&#46; After this episode&#44; no reproductive disorders were observed&#46; During 2020&#44; 27 healthy puppies were born&#46;</p><p id="par0030" class="elsevierStylePara elsevierViewall">The success of the antibiotic treatment used&#44; the absence of other pathogens causing infertility&#44; the detection of <span class="elsevierStyleItalic">Mycoplasmas</span>&#44; the changes in Gucci&#39;s sperm analysis and the recovery from the bitches&#8217; previous subfertility after the antibiotic treatments&#44; suggest that <span class="elsevierStyleItalic">Mycoplasma</span> species were associated with that subfertility episode in this dog kennel&#46; Although the results of Gucci&#39;s sperm analysis improved after treatment with doxycycline&#44; it was only after the treatment with azithromycin that successful pregnancies were achieved&#46; In this regard&#44; azithromycin&#44; doxycycline&#44; enrofloxacin&#44; marbofloxacin&#44; minocycline&#44; orbifloxacin and pradofloxacin have been pointed out as effective against <span class="elsevierStyleItalic">Mycoplasma-</span>associated respiratory infections in dogs<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">9</span></a>&#46; However&#44; azithromycin would be the most suitable antibiotic for the treatment of infections caused by <span class="elsevierStyleItalic">Mycoplasma</span><a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">9</span></a>&#46; Although <span class="elsevierStyleItalic">M&#46; canis</span> is the predominant species identified from the vaginas of fertile and infertile bitches<a class="elsevierStyleCrossRefs" href="#bib0110"><span class="elsevierStyleSup">6&#44;10&#44;12</span></a> and <span class="elsevierStyleItalic">Mycoplasma</span><span class="elsevierStyleItalic">cynos</span> had been associated with pneumonia<a class="elsevierStyleCrossRefs" href="#bib0100"><span class="elsevierStyleSup">4&#44;16</span></a>&#44; we did not find any of those species&#46; <span class="elsevierStyleItalic">M&#46; spumans</span> and <span class="elsevierStyleItalic">M&#46; maculosum</span> have been identified from the respiratory and genitourinary systems of dogs with and without respiratory infection<a class="elsevierStyleCrossRefs" href="#bib0085"><span class="elsevierStyleSup">1&#44;2&#44;7</span></a> and from fertile and infertile dogs<a class="elsevierStyleCrossRefs" href="#bib0090"><span class="elsevierStyleSup">2&#44;6&#44;10</span></a>&#46; These antecedents might explain the subfertility episode in this kennel&#44; the fatal pneumonia of Catalina&#39;s puppy and even&#44; the tracheal aplasia of Wish&#39;s puppy&#46; Further experimental studies are necessary to confirm our results&#46; With regard to <span class="elsevierStyleItalic">M&#46; leopharyngis</span>&#44; we did not find any antecedents about its presence in dogs&#44; since together with other six <span class="elsevierStyleItalic">Mycoplasma</span> species&#44; it affects felines<a class="elsevierStyleCrossRef" href="#bib0095"><span class="elsevierStyleSup">3</span></a>&#46; Considering the high similarity &#40;98&#46;6&#37;&#41; in the 16S-23S rRNA ITS region between <span class="elsevierStyleItalic">M&#46; maculosum</span> and <span class="elsevierStyleItalic">M&#46; leopharyngis</span><a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">14</span></a>&#44; the detected species could be <span class="elsevierStyleItalic">M&#46; maculosum&#46;</span> However&#44; the presence of <span class="elsevierStyleItalic">M&#46; leopharyngis</span> in the urogenital tract of dogs cannot be strictly ruled out&#46; The analysis of other target sequences would have allowed the unambiguous identification of <span class="elsevierStyleItalic">Mycoplasma</span> species&#46; Taking into account the phylogenetic relatedness and pairwise sequence similarities of the species within the family <span class="elsevierStyleItalic">Mycoplasmataceae</span>&#44; a reliable and useful taxonomic tool&#44; analyzing 16S-23S rRNA ITS&#44; RNA polymerase beta subunit &#40;rpoB&#41; and 16S rRNA genes&#44; has been proposed<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">15</span></a>&#46; Regrettably&#44; <span class="elsevierStyleItalic">rpoB</span> and <span class="elsevierStyleItalic">16S rRNA</span> genes were not addressed in this study&#46; In this way&#44; the analysis of the three-target sequence has been pointed out as a valuable tool for routine analyses<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">15</span></a>&#46;</p><p id="par0035" class="elsevierStylePara elsevierViewall">Unfortunately&#44; veterinarians do not often consider <span class="elsevierStyleItalic">Mycoplasma</span> in the differential diagnosis of respiratory and reproductive diseases in dogs&#46; For this reason&#44; <span class="elsevierStyleItalic">Mycoplasma</span> detection either by culture or molecular-based tests is rarely requested&#46; From a practical point of view&#44; the detection of these agents is important for the implementation of an adequate antibiotic treatment&#44; beyond the identification of the involved species&#46; Due to the lack of cell wall in <span class="elsevierStyleItalic">Mycoplasma</span>&#44; they are inherently resistant to &#946;-lactam antibiotics&#46; In this report&#44; prolonged therapy with doxycycline and azithromycin demonstrated to be effective against <span class="elsevierStyleItalic">Mycoplasma</span> infections&#44; agreeing with previous data<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">9</span></a>&#46; In spite of the limitation of this study&#44; since <span class="elsevierStyleItalic">Mycoplasma</span> culture was not tried&#44; the importance of the diagnosis of these agents was demonstrated&#46;</p><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Conflict of interest</span><p id="par0040" class="elsevierStylePara elsevierViewall">The author declares that he has no conflicts of interest&#46;</p></span></span>"
    "textoCompletoSecciones" => array:1 [
      "secciones" => array:8 [
        0 => array:3 [
          "identificador" => "xres1688163"
          "titulo" => "Highlights"
          "secciones" => array:1 [
            0 => array:1 [
              "identificador" => "abst0005"
            ]
          ]
        ]
        1 => array:3 [
          "identificador" => "xres1688164"
          "titulo" => "Abstract"
          "secciones" => array:1 [
            0 => array:1 [
              "identificador" => "abst0010"
            ]
          ]
        ]
        2 => array:2 [
          "identificador" => "xpalclavsec1497090"
          "titulo" => "Keywords"
        ]
        3 => array:3 [
          "identificador" => "xres1688162"
          "titulo" => "Resumen"
          "secciones" => array:1 [
            0 => array:1 [
              "identificador" => "abst0015"
            ]
          ]
        ]
        4 => array:2 [
          "identificador" => "xpalclavsec1497091"
          "titulo" => "Palabras clave"
        ]
        5 => array:2 [
          "identificador" => "sec0005"
          "titulo" => "Conflict of interest"
        ]
        6 => array:2 [
          "identificador" => "xack595658"
          "titulo" => "Acknowledgements"
        ]
        7 => array:1 [
          "titulo" => "References"
        ]
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2020-09-16"
    "fechaAceptado" => "2021-04-12"
    "PalabrasClave" => array:2 [
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Keywords"
          "identificador" => "xpalclavsec1497090"
          "palabras" => array:6 [
            0 => "Dogs"
            1 => "Fertility disorders"
            2 => "Urogenital tract"
            3 => "<span class="elsevierStyleItalic">Mycoplasma maculosum</span>"
            4 => "<span class="elsevierStyleItalic">Mycoplasma spumans</span>"
            5 => "Antibiotic treatment"
          ]
        ]
      ]
      "es" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Palabras clave"
          "identificador" => "xpalclavsec1497091"
          "palabras" => array:6 [
            0 => "Perros"
            1 => "Trastornos de fertilidad"
            2 => "Tracto urogenital"
            3 => "<span class="elsevierStyleItalic">Mycoplasma maculosum</span>"
            4 => "<span class="elsevierStyleItalic">Mycoplasma spumans</span>"
            5 => "Tratamiento antibi&#243;tico"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "highlights" => array:2 [
      "titulo" => "Highlights"
      "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall"><ul class="elsevierStyleList" id="lis0005"><li class="elsevierStyleListItem" id="lsti0005"><span class="elsevierStyleLabel">&#8226;</span><p id="par0005" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Mycoplasma maculosum</span> and <span class="elsevierStyleItalic">Mycoplasma spumans</span> were detected by PCR in dogs&#46;</p></li><li class="elsevierStyleListItem" id="lsti0010"><span class="elsevierStyleLabel">&#8226;</span><p id="par0010" class="elsevierStylePara elsevierViewall">After antibiotic treatment reproductive disorders disappeared&#46;</p></li><li class="elsevierStyleListItem" id="lsti0015"><span class="elsevierStyleLabel">&#8226;</span><p id="par0015" class="elsevierStylePara elsevierViewall">Veterinarians not often consider <span class="elsevierStyleItalic">Mycoplasma</span> in the differential diagnosis of reproductive diseases&#46;</p></li></ul></p></span>"
    ]
    "resumen" => array:2 [
      "en" => array:2 [
        "titulo" => "Abstract"
        "resumen" => "<span id="abst0010" class="elsevierStyleSection elsevierViewall"><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">The aim of this short communication is to describe a case of subfertility and other anomalies associated with the presence of <span class="elsevierStyleItalic">Mycoplasma spumans</span> and <span class="elsevierStyleItalic">Mycoplasma maculosum</span> in a Bernese Mountain Dog kennel&#46; After the arrival of two dogs from abroad&#44; some fertility disorders&#44; such as unsuccessful mating&#44; pregnancy losses and abnormal sperm analysis results&#44; were observed&#46; Two consecutive samplings &#40;vaginal swabs&#41; of three and two bitches with problems&#44; respectively&#44; were performed and <span class="elsevierStyleItalic">M&#46; spumans</span> and <span class="elsevierStyleItalic">M&#46; maculosum</span> were identified by PCR and sequencing&#46; After treatment for 15 days with doxycycline and 9 days with azithromycin&#44; successful pregnancies were achieved and the results of the sperm analyses were reversed&#46; Considering that no other infectious agents causing subfertility problems were detected and that no management measures or other medication apart from these antibiotics were applied&#44; it was concluded that fertility problems were due to the presence of these two <span class="elsevierStyleItalic">Mycoplasma</span> species&#46;</p></span>"
      ]
      "es" => array:2 [
        "titulo" => "Resumen"
        "resumen" => "<span id="abst0015" class="elsevierStyleSection elsevierViewall"><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">El objetivo de esta comunicaci&#243;n es describir un caso de subfertilidad y otras anomal&#237;as asociadas a la presencia de <span class="elsevierStyleItalic">Mycoplasma spumans</span> y <span class="elsevierStyleItalic">Mycoplasma maculosum</span> en un criadero de perros boyero de Berna&#46; Despu&#233;s de la entrada de 2 perros del exterior&#44; comenzaron a observarse algunos trastornos de la fertilidad&#44; como apareamientos infructuosos&#44; p&#233;rdidas de pre&#241;ez y resultados anormales en el an&#225;lisis esperm&#225;tico&#46; Se realizaron 2 muestreos consecutivos &#40;hisopados vaginales&#41; de 3 y 2 perras con problemas&#44; respectivamente&#44; y se identific&#243; <span class="elsevierStyleItalic">M&#46; spumans</span> y <span class="elsevierStyleItalic">M&#46; maculosum</span> mediante PCR y secuenciaci&#243;n&#46; Despu&#233;s del tratamiento con doxiciclina y azitromicina durante 15 y 9 d&#237;as&#44; respectivamente&#44; se lograron obtener pre&#241;eces exitosas y se revirtieron los resultados del espermograma&#46; Considerando que no se detectaron otros agentes infecciosos causantes de problemas de subfertilidad y que no se aplic&#243; ninguna medida de manejo ni otra medicaci&#243;n&#44; aparte de los tratamientos antibi&#243;ticos mencionados&#44; se concluy&#243; que los problemas de fertilidad se debieron a la presencia de estas 2 especies de <span class="elsevierStyleItalic">Mycoplasma</span>&#46;</p></span>"
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0015"
          "bibliografiaReferencia" => array:16 [
            0 => array:3 [
              "identificador" => "bib0085"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Previously uncharacterized <span class="elsevierStyleItalic">Mycoplasma</span> isolates from an investigation of canine pneumonia"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "D&#46; Armstrong"
                            1 => "J&#46;G&#46; Tully"
                            2 => "B&#46; Yu"
                            3 => "V&#46; Morton"
                            4 => "M&#46;H&#46; Friedman"
                            5 => "L&#46; Steger"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Infect Immun"
                        "fecha" => "1970"
                        "volumen" => "1"
                        "paginaInicial" => "1"
                        "paginaFinal" => "7"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0090"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Isolation and rapid identification of <span class="elsevierStyleItalic">Mycoplasma</span> species from canine tissues by plate immunofluorescence"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "M&#46;F&#46; Barile"
                            1 => "R&#46;A&#46; Del Giudice"
                            2 => "T&#46;R&#46; Carski"
                            3 => "H&#46;M&#46; Yamashiroya"
                            4 => "J&#46;A&#46; Verna"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.3181/00379727-134-34747"
                      "Revista" => array:6 [
                        "tituloSerie" => "Proc Soc Exp Biol Med"
                        "fecha" => "1970"
                        "volumen" => "134"
                        "paginaInicial" => "146"
                        "paginaFinal" => "148"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/4912557"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0095"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Taxonomy of the feline mycoplasmas <span class="elsevierStyleItalic">Mycoplasma felifaucium</span>&#44; <span class="elsevierStyleItalic">Mycoplasma feliminutum</span>&#44; <span class="elsevierStyleItalic">Mycoplasma felis</span>&#44; <span class="elsevierStyleItalic">Mycoplasma gateae</span>&#44; <span class="elsevierStyleItalic">Mycoplasma leocaptivus</span>&#44; <span class="elsevierStyleItalic">Mycoplasma leopharyngis</span>&#44; and <span class="elsevierStyleItalic">Mycoplasma simbae</span> by 16S rRNA gene sequence comparisons"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "D&#46;R&#46; Brown"
                            1 => "G&#46;S&#46; McLaughlin"
                            2 => "M&#46;B&#46; Brown"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1099/00207713-45-3-560"
                      "Revista" => array:6 [
                        "tituloSerie" => "Int J Syst Bacteriol"
                        "fecha" => "1995"
                        "volumen" => "45"
                        "paginaInicial" => "560"
                        "paginaFinal" => "564"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8590684"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib0100"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "<span class="elsevierStyleItalic">Mycoplasmas</span> associated with canine infectious respiratory disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:7 [
                            0 => "V&#46;J&#46; Chalker"
                            1 => "W&#46;M&#46; Owen"
                            2 => "C&#46; Paterson"
                            3 => "E&#46; Barker"
                            4 => "H&#46; Brooks"
                            5 => "A&#46;N&#46; Rycroft"
                            6 => "J&#46; Brownlie"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1099/mic.0.26848-0"
                      "Revista" => array:6 [
                        "tituloSerie" => "Microbiology"
                        "fecha" => "2004"
                        "volumen" => "150"
                        "paginaInicial" => "3491"
                        "paginaFinal" => "3497"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15470126"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib0105"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Canine <span class="elsevierStyleItalic">mycoplasmas</span>"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "V&#46;J&#46; Chalker"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.rvsc.2004.10.002"
                      "Revista" => array:6 [
                        "tituloSerie" => "Res Vet Sci"
                        "fecha" => "2005"
                        "volumen" => "79"
                        "paginaInicial" => "1"
                        "paginaFinal" => "8"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15894017"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib0110"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The genital <span class="elsevierStyleItalic">Mycoplasma</span> and <span class="elsevierStyleItalic">Ureaplasma</span> flora of healthy and diseased dogs"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "P&#46;A&#46; Doig"
                            1 => "H&#46;L&#46; Ruhnke"
                            2 => "W&#46;T&#46; Bosu"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Can J Comp Med"
                        "fecha" => "1981"
                        "volumen" => "45"
                        "paginaInicial" => "233"
                        "paginaFinal" => "328"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/7340908"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib0115"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The isolation of organisms of the pleuropneumonia group from dogs"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "D&#46;G&#46; Edward"
                            1 => "W&#46;A&#46; Fitzgerald"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1099/00221287-5-3-566"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Gen Microbiol"
                        "fecha" => "1951"
                        "volumen" => "5"
                        "paginaInicial" => "566"
                        "paginaFinal" => "575"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/14873903"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib0120"
              "etiqueta" => "8"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "BioEdit&#58; a user-friendly biological sequence alignment editor and analysis program for Windows 95&#47;98&#47;NT"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "T&#46;A&#46; Hall"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Nucleic Acids Symp Ser"
                        "fecha" => "1999"
                        "volumen" => "41"
                        "paginaInicial" => "95"
                        "paginaFinal" => "98"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib0125"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Antimicrobial use guidelines for treatment of respiratory tract disease in dogs and cats&#58; antimicrobial guidelines working group of the International Society for Companion Animal Infectious Diseases"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:11 [
                            0 => "M&#46;R&#46; Lappin"
                            1 => "J&#46; Blondeau"
                            2 => "D&#46; Boothe"
                            3 => "E&#46;B&#46; Breitschwerdt"
                            4 => "L&#46; Guardabassi"
                            5 => "D&#46;H&#46; Lloyd"
                            6 => "M&#46;G&#46; Papich"
                            7 => "S&#46;C&#46; Rankin"
                            8 => "J&#46;E&#46; Sykes"
                            9 => "J&#46; Turnidge"
                            10 => "J&#46;S&#46; Weese"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/jvim.14627"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Vet Intern Med"
                        "fecha" => "2017"
                        "volumen" => "31"
                        "paginaInicial" => "279"
                        "paginaFinal" => "284"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/28185306"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib0130"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Genital <span class="elsevierStyleItalic">mycoplasmas</span> of healthy bitches"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "Z&#46; Maksimovi&#263;"
                            1 => "A&#46; Maksimovi&#263;"
                            2 => "A&#46; Halilba&#353;i&#263;"
                            3 => "M&#46; Rifatbegovi&#263;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1177/1040638718778745"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Vet Diagn Invest"
                        "fecha" => "2018"
                        "volumen" => "30"
                        "paginaInicial" => "651"
                        "paginaFinal" => "653"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/29790451"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib0135"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Molecular biology and pathogenicity of <span class="elsevierStyleItalic">mycoplasmas</span>"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "S&#46; Razin"
                            1 => "D&#46; Yogev"
                            2 => "Y&#46; Naot"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1128/MMBR.62.4.1094-1156.1998"
                      "Revista" => array:6 [
                        "tituloSerie" => "Microbiol Mol Biol Rev"
                        "fecha" => "1998"
                        "volumen" => "62"
                        "paginaInicial" => "1094"
                        "paginaFinal" => "1156"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9841667"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            11 => array:3 [
              "identificador" => "bib0140"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Identification and differentiation of canine <span class="elsevierStyleItalic">Mycoplasma</span> isolates by 16S-23S rDNA PCR-RFLP"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "J&#46; Spergser"
                            1 => "R&#46; Rosengarten"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.vetmic.2007.04.045"
                      "Revista" => array:6 [
                        "tituloSerie" => "Vet Microbiol"
                        "fecha" => "2007"
                        "volumen" => "125"
                        "paginaInicial" => "170"
                        "paginaFinal" => "174"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17544231"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib0145"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "A polymerase chain reaction-based method for detecting <span class="elsevierStyleItalic">Mycoplasma&#47;Acholeplasma</span> contaminants in cell culture"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "J&#46; Tang"
                            1 => "M&#46; Hu"
                            2 => "S&#46; Lee"
                            3 => "R&#46; Roblin"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/s0167-7012(99)00107-4"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Microbiol Methods"
                        "fecha" => "2000"
                        "volumen" => "39"
                        "paginaInicial" => "121"
                        "paginaFinal" => "126"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10576701"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib0150"
              "etiqueta" => "14"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Sequencing of the intergenic 16S-23S rRNA spacer &#40;ITS&#41; region of <span class="elsevierStyleItalic">Mollicutes</span> species and their identification using microarray-based assay and DNA sequencing"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "D&#46;V&#46; Volokhov"
                            1 => "J&#46; George"
                            2 => "S&#46;X&#46; Liu"
                            3 => "P&#46; Ikonomi"
                            4 => "C&#46; Anderson"
                            5 => "V&#46; Chizhikov"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1007/s00253-005-0280-7"
                      "Revista" => array:6 [
                        "tituloSerie" => "Appl Microbiol Biotechnol"
                        "fecha" => "2006"
                        "volumen" => "71"
                        "paginaInicial" => "680"
                        "paginaFinal" => "698"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16470366"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            14 => array:3 [
              "identificador" => "bib0155"
              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "RNA polymerase beta subunit &#40;<span class="elsevierStyleItalic">rpoB</span>&#41; gene and the 16S-23S rRNA intergenic transcribed spacer region &#40;ITS&#41; as complementary molecular markers in addition to the 16S rRNA gene for phylogenetic analysis and identification of the species of the family <span class="elsevierStyleItalic">Mycoplasmataceae</span>"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "D&#46;V&#46; Volokhov"
                            1 => "V&#46; Simonyan"
                            2 => "M&#46;K&#46; Davidson"
                            3 => "V&#46;E&#46; Chizhikov"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.ympev.2011.11.002"
                      "Revista" => array:6 [
                        "tituloSerie" => "Mol Phylogenet Evol"
                        "fecha" => "2012"
                        "volumen" => "62"
                        "paginaInicial" => "515"
                        "paginaFinal" => "528"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22115576"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            15 => array:3 [
              "identificador" => "bib0160"
              "etiqueta" => "16"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Lethal bronchopneumonia caused by <span class="elsevierStyleItalic">Mycoplasma cynos</span> in a litter of golden retriever puppies"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "F&#46; Zeugswetter"
                            1 => "H&#46; Weissenb&#246;ck"
                            2 => "S&#46; Shibly"
                            3 => "J&#46; Hassan"
                            4 => "J&#46; Spergser"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1136/vr.161.18.626"
                      "Revista" => array:6 [
                        "tituloSerie" => "Vet Rec"
                        "fecha" => "2007"
                        "volumen" => "161"
                        "paginaInicial" => "626"
                        "paginaFinal" => "627"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17982143"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
    "agradecimientos" => array:1 [
      0 => array:4 [
        "identificador" => "xack595658"
        "titulo" => "Acknowledgements"
        "texto" => "<p id="par0045" class="elsevierStylePara elsevierViewall">This study was financially supported in part by FONCyT-ANPCyT-MinCyT&#44; Rep&#250;blica Argentina &#91;PICT 02148&#47;2018&#93;&#44; PPI 2019-2021 SeCyT-UNRC and IDi division of ACERCA Laboratorio&#46; The author is deeply grateful to the owners of the Bernese Mountain dog kennel&#44; Silvina Otero and Gustavo Pisani and to the veterinarians Ricardo Abaurrea&#44; Carlos Enrique Sorribas and Isabel Zubiros&#46;</p>"
        "vista" => "all"
      ]
    ]
  ]
  "idiomaDefecto" => "en"
  "url" => "/03257541/0000005400000001/v1_202204020607/S0325754121000523/v1_202204020607/en/main.assets"
  "Apartado" => array:4 [
    "identificador" => "37861"
    "tipo" => "SECCION"
    "en" => array:2 [
      "titulo" => "Microbiolog&#237;a cl&#237;nica y enfermedades infecciosas"
      "idiomaDefecto" => true
    ]
    "idiomaDefecto" => "en"
  ]
  "PDF" => "https://static.elsevier.es/multimedia/03257541/0000005400000001/v1_202204020607/S0325754121000523/v1_202204020607/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0325754121000523?idApp=UINPBA00004N"
]

Article information

ISSN: 03257541
Original language: English

DOI:10.1016/j.ram.2021.04.001

The statistics are updated each day

Year/Month	Html	Pdf	Total

2024 November	5	1	6
2024 October	26	21	47
2024 September	58	40	98
2024 August	40	29	69
2024 July	42	33	75
2024 June	32	18	50
2024 May	34	24	58
2024 April	45	18	63
2024 March	37	29	66
2024 February	29	42	71
2024 January	27	34	61
2023 December	27	30	57
2023 November	42	34	76
2023 October	37	36	73
2023 September	25	17	42
2023 August	22	15	37
2023 July	23	36	59
2023 June	50	22	72
2023 May	59	32	91
2023 April	50	21	71
2023 March	23	24	47
2023 February	22	31	53
2023 January	42	26	68
2022 December	54	25	79
2022 November	35	21	56
2022 October	29	24	53
2022 September	35	51	86
2022 August	33	46	79
2022 July	29	31	60
2022 June	35	28	63
2022 May	33	39	72
2022 April	41	17	58
2022 March	33	14	47
2022 February	19	10	29
2022 January	24	11	35
2021 December	18	11	29
2021 November	7	8	15
2021 October	13	13	26
2021 September	16	9	25
2021 August	10	4	14
2021 July	11	10	21
2021 June	8	2	10

Show all

Indexed in:

Follow us:

Statistics

Indexed in:

Follow us:

Statistics

Subscribe to our newsletter