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Brief report
Saliva screening of health care workers for SARS-CoV-2 detection
Análisis del personal de salud a partir de saliva para detección de SARS-CoV-2
Marcela Echavarriaa,b,
Corresponding author
mechavarria@cemic.edu.ar

Corresponding author.
, Noelia S. Reyesa, Pamela E. Rodrigueza, Carmen Ricartea, Martin Ypasc, Alejandro Seoanec, Marcia Quercid, Marianela Brizioe, Martin E. Stryjewskie, Guadalupe Carballala
a Virology Unit (UNVIR-CONICET), Centro de Educación Médica e Investigaciones Clínicas (CEMIC) University Hospital, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
b Virology Laboratory, Centro de Educación Médica e Investigaciones Clínicas (CEMIC) University Hospital, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
c Emergency Service, Centro de Educación Médica e Investigaciones Clínicas (CEMIC) University Hospital, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
d Division of Infectious Disease, Centro de Educación Médica e Investigaciones Clínicas (CEMIC) University Hospital, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
e Department of Medicine, Centro de Educación Médica e Investigaciones Clínicas (CEMIC) University Hospital, Ciudad Autónoma de Buenos Aires, Buenos Aires, Argentina
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is the most common respiratory sample utilized for SARS-CoV-2 diagnosis&#46; However&#44; NPS is associated with subjects&#8217; discomfort and further exposure to viral aerosols during sample collection<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">8</span></a>&#46; Saliva testing is a non-invasive test to detect SARS-CoV-2 that avoids further HCW exposure&#46; Saliva testing proved to be as sensitive as NPS in detecting SARS-CoV-2 in symptomatic patients<a class="elsevierStyleCrossRefs" href="#bib0115"><span class="elsevierStyleSup">6&#44;7</span></a>&#46; However&#44; the role of saliva testing among asymptomatic HCWs is still to be determined&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall">The objective of this study was to determine the performance of the saliva test to screen for SARS-CoV-2 in HCWs&#46;</p><p id="par0045" class="elsevierStylePara elsevierViewall">A descriptive prospective cohort study in HCWs was conducted at CEMIC University Hospital&#44; Buenos Aires&#44; Argentina&#46; Voluntary SARS-CoV-2 testing in saliva was performed in both hospital sites&#58; CEMIC Saavedra and CEMIC Pombo&#46; HCWs were enrolled at 12 different hospital settings&#46; These settings were classified as &#8220;high&#8221;&#44; &#8220;middle&#8221; or &#8220;low&#8221; in terms of exposure risk to SARS-CoV-2&#46; High exposure risk settings included&#58; emergency room&#44; intensive care unit &#40;ICU&#41;&#44; infectious diseases&#44; and personnel assisting COVID-19 positive inpatients or specimens &#40;technicians&#44; nurses&#44; physicians&#44; physical therapists&#44; laboratory&#41;&#46; Middle exposure risk settings included&#58; personnel assisting COVID-19 negative inpatients &#40;technicians&#44; nurses&#44; physicians&#44; physical therapists&#41;&#46; Low exposure risk areas included those without contact with patients or clinical specimens &#40;pharmacy&#44; administration&#41;&#46;</p><p id="par0050" class="elsevierStylePara elsevierViewall">An electronic form with personal data and information related to COVID-19 disease or exposure was collected at the time of sampling&#46; Clinical follow-up for any signs or symptoms compatible with COVID-19 was obtained from participants for two weeks from the initial collection of saliva&#46; A nasopharyngeal swab and a blood sample for serology were obtained from participants with positive RT-PCR results for SARS-CoV-2&#46;</p><p id="par0055" class="elsevierStylePara elsevierViewall">The study was approved by the CEMIC Ethics Committee &#40;Protocol&#58; 1298&#47;20&#41; and electronic informed consent was obtained from all participants&#46;</p><p id="par0060" class="elsevierStylePara elsevierViewall">At study entry&#44; a self-collected saliva sample was obtained for SARS-CoV-2 diagnosis&#46; Participants were instructed on how to collect the sample in a sterile plastic container without viral transport medium&#46; Samples were conserved at 4<span class="elsevierStyleHsp" style=""></span>&#176;C until processed in a biosafety cabinet within 12<span class="elsevierStyleHsp" style=""></span>h of arrival&#46; Viscous saliva samples were mechanically disrupted by adding 500<span class="elsevierStyleHsp" style=""></span>&#956;l viral transport medium &#91;Minimun Essential Medium &#40;Gibco&#41;&#59; <span class="elsevierStyleSmallCaps">l</span>-Glutamin 200<span class="elsevierStyleHsp" style=""></span>mM&#59; HEPES 1<span class="elsevierStyleHsp" style=""></span>N&#59; bovine serum albumin 5&#37; &#40;Sigma&#41; sodium bicarbonate 7&#46;5&#37;&#59; penicillin&#44; streptomycin and amphotericin &#40;pH<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>7&#46;2&#41;&#93;&#46; Participants in whom SARS-CoV-2 was detected by PCR in the initial sample of saliva were requested to provide additional saliva samples and NPS&#46; NPS were obtained and placed in a sterile tube containing 2<span class="elsevierStyleHsp" style=""></span>ml viral transport media&#46;</p><p id="par0065" class="elsevierStylePara elsevierViewall">Nucleic acid was extracted from 100<span class="elsevierStyleHsp" style=""></span>&#956;l saliva sample and eluted in 15<span class="elsevierStyleHsp" style=""></span>&#956;l using manual columns &#40;Quick-RNA&#8482; Viral Kit&#46; Zymo Research CORP&#46;&#41; following the manufacturer&#39;s recommendation&#46; One-step real-time multiplex RT-PCR laboratory-optimized was performed&#44; targeting the SARS-CoV-2 E gene and the human RNAse P gene as quality control<a class="elsevierStyleCrossRefs" href="#bib0105"><span class="elsevierStyleSup">4&#44;6</span></a>&#46; A positive result was considered when the cycle threshold &#40;Ct&#41; value for the SARS-CoV-2 E gene was lower than 40 and the human RNAse gene was positive&#46; Analytical sensitivity was 1<span class="elsevierStyleHsp" style=""></span>copy&#47;&#956;l&#46; Discrepant PCR results were tested using a commercial RT-PCR kit that amplifies the SARS-CoV-2 E gene and the S gene and includes an internal amplification control &#40;Real Star&#174; SARS-CoV-2 RT-PCR Kit 1&#46;0&#46; Altona Diagnostics Argentina S&#46;R&#46;L&#46;&#41;&#46; Real time assays were performed in a CFX 96 Deep Well&#8482; Real Time System &#40;BioRad&#41;&#46;</p><p id="par0070" class="elsevierStylePara elsevierViewall">Serum samples were analyzed for the presence of SARS-CoV-2 specific IgM and IgG antibodies using ELISA kit COVIDAR IgM&#47;IgG&#46; The assay uses a trimer stabilized spike protein and the RBD &#40;FIL-CONICET-Laboratorio Lemos&#44; Argentina&#41;<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">9</span></a>&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">Ct values of saliva vs&#46; those observed in NPS were compared with a paired <span class="elsevierStyleItalic">Student&#39;s</span><span class="elsevierStyleItalic">t</span>-test&#46; A two-sided <span class="elsevierStyleItalic">p</span>-value of &#60;0&#46;05 was considered significant &#40;GraphPad Prism 8&#46;0&#46;2&#44; San Diego California&#44; USA&#41;&#46;</p><p id="par0080" class="elsevierStylePara elsevierViewall">From September 9th to October 13th 2020&#44; 100 asymptomatic&#47;presymptomatic HCWs were enrolled in the study&#46; Demographic characteristics were described as appropriate&#46; Almost two thirds were female &#40;67&#37;&#41; and the median age was 37 years old &#40;IQR<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>31&#8722;46&#41;&#46; Around half of the participants &#40;53&#37;&#41; described having been in contact with COVID-19 patients at some point and 3&#46;8&#37; of these participants reported previous SARS-CoV-2 infection&#44; but were negative in saliva or NPS sample at the time of this screening&#46;</p><p id="par0085" class="elsevierStylePara elsevierViewall">Of 100 HCWs&#44; 6 asymptomatic subjects &#40;6&#37;&#41; were positive in saliva &#40;2 physicians&#44; 2 nurses&#44; and 2 administrative personnel&#44; respectively&#41;&#46; All positive HCWs belonged to high exposure settings&#46; Upon the initial results in saliva&#44; all these HCWs were separated and licensed from work on the same day of sample collection&#46;</p><p id="par0090" class="elsevierStylePara elsevierViewall">NPS from positive patients were mostly obtained within 24&#8211;48<span class="elsevierStyleHsp" style=""></span>h from saliva testing&#46; Five of six HCWs with positive salivawere positive in subsequent NPS &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#44; no significant differences between Ct values &#40;saliva vs&#46; NPS&#41; were observed &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;3173&#41;&#46; A patient with negative NSP showed a positive saliva test with a high Ct value&#46; This result was confirmed in duplicate and also using a different RT-PCR assay &#40;home-brew and commercial kit Altona Diagnostic&#41;&#46; A follow-up saliva sample obtained 48<span class="elsevierStyleHsp" style=""></span>h later was also positive but with a higher Ct value&#46; Among HCWs with positive saliva&#44; 4&#47;6 developed signs and symptoms compatible with COVID-19 within 2&#8211;4 days after the initial collection of saliva&#46; The other 2 HCWs remained asymptomatic&#46; Median Ct values in saliva were lower in pre-symptomatic &#40;mean<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>28&#46;36&#59; range&#58; 15&#46;26&#8211;36&#46;80&#41; versus asymptomatic &#40;mean<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>34&#46;63&#59; range&#58; 33&#46;71&#8211;35&#46;53&#41; HCW &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;4033&#41;&#46; Of 4 pre-symptomatic HCWs with positive saliva&#44; 3 seroconverted&#46; None of the two HCWs who remained asymptomatic seroconverted &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; A follow-up saliva sample obtained from the asymptomatic HCW &#40;ID &#35;4&#41; confirmed positivity although with a higher Ct value &#40;Ct<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>37&#41;&#46; None of 94 HCWs who tested negative for SARS-CoV-2 in saliva developed symptoms&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0095" class="elsevierStylePara elsevierViewall">Expanding screening protocols for detecting pre-symptomatic or asymptomatic carriers among HCWs is critical to reduce the spread of SARS-CoV-2 in the hospital setting&#46; Some of the challenges to establish these protocols are related to logistical issues&#44; turnaround times and further exposure to HCW during sampling&#46; Self-collected saliva provides an option to facilitate sample collection&#44; reduce discomfort and minimize HCW exposure&#46; There are few reports utilizing saliva samples in the routine testing of asymptomatic or pre-symptomatic patients&#44; including HCWs<a class="elsevierStyleCrossRef" href="#bib0160"><span class="elsevierStyleSup">15</span></a>&#46; Generally&#44; saliva tests were utilized for follow-up after a positive NPS but not as a primary screening method<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">13</span></a>&#46; In this study&#44; we have used saliva samples as a primary screening method to detect SARS-CoV-2 among HCWs&#46; The incidence of SARS-CoV-2 in pre-symptomatic&#47;asymptomatic HCWs reaching 6&#37; was higher than expected&#46; Most of our positive cases corresponded to HCWs performing work activities in high or middle risk areas&#46; Some authors reported negative results for saliva testing among HCWs<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">1</span></a> while others found percentages ranging from 1&#46;5 to 12&#46;5&#37; using NPS in those asymptomatic<a class="elsevierStyleCrossRefs" href="#bib0145"><span class="elsevierStyleSup">12&#44;14&#44;16</span></a>&#46; Our screening results in saliva are comparable with studies using NPS&#46; A high correlation between saliva and NPS was previously demonstrated in symptomatic patients using a highly sensitive home brew RT-PCR<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">6</span></a>&#46; In this study&#44; saliva was a useful&#44; valuable and easy tool for SARS-CoV-2 screening and for identifying pre-symptomatic and asymptomatic individuals&#46; Importantly&#44; rapid identification of positive HCWs permitted early licensing avoiding potential SARS-CoV-2 spreaders within the hospital setting&#46; Furthermore&#44; saliva positivity anticipated clinical disease up to 3 days before symptom onset&#46; Long-term SARS-CoV-2 RNA shedding can occur in saliva&#46; In our study&#44; the only patient who gave a discrepant result between NPS and saliva and remained asymptomatic showed a follow-up saliva with a higher Ct value&#44; probably representing prolonged shedding in this sample type<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">17</span></a>&#46;</p><p id="par0100" class="elsevierStylePara elsevierViewall">Of 6 positive HCWs&#44; 2 remained asymptomatic&#46; Studies evaluating nasopharyngeal swabs in HCWs demonstrated that 15&#37; to 57&#37; remained asymptomatic<a class="elsevierStyleCrossRefs" href="#bib0095"><span class="elsevierStyleSup">2&#44;11</span></a>&#46; Seroconversion was demonstrated in most of our symptomatic patients&#46; The patient who did not seroconvert even 90 days after symptom onset had a very mild disease&#46; This finding is not unexpected since IgG titers have been associated with disease severity<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">9</span></a>&#46; In addition&#44; lack of seroconversion was previously described in 17&#37; of HCWs infected with SARS-CoV-2<a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">3</span></a>&#46;</p><p id="par0105" class="elsevierStylePara elsevierViewall">Saliva is an easy to obtain sample that can be self-collected&#46; It is especially convenient for screening in individuals without respiratory symptoms&#46; Furthermore&#44; special attention should be given to asymptomatic individuals who may spread the virus more easily due to higher human interaction than symptomatic patients<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">10</span></a>&#46; Interestingly&#44; previous studies with saliva testing have shown discrepant results&#46; We believe the reason for such discrepancy is related to sample processing and the PCR techniques employed&#46; We have systematically conducted and applied an optimized PCR &#40;home brew&#41;&#46; Our technique includes mechanical disruption of the saliva&#44; no addition of any stabilizer or buffer and an increased concentration of magnesium &#40;3&#46;8<span class="elsevierStyleHsp" style=""></span>mM&#41; in the PCR mix as well as modifications in cycling conditions<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">6</span></a>&#46; Therefore&#44; the optimization of our PCR resulted in an increased analytical and clinical sensitivity&#46; Furthermore&#44; this home brew assay incurred less costs than those related to commercial PCR kits &#40;data not shown&#41;&#46;</p><p id="par0110" class="elsevierStylePara elsevierViewall">This study has several limitations&#46; Our investigation was based on voluntary participation and this may have introduced some voluntary bias&#46; In addition&#44; other populations need to be tested to define the role of saliva to detect SARS-CoV-2 in symptomatic&#44; pre-symptomatic or asymptomatic subjects&#46; Finally&#44; our sample size was not large&#46; However&#44; the number was deemed to be epidemiologically meaningful&#46; Supporting this concept&#44; we were able to detect 6&#37; of HCWs infected with SARS-CoV-2 while they were pre-symptomatic&#47;asymptomatic&#46;</p><p id="par0115" class="elsevierStylePara elsevierViewall">The screening protocol with the saliva sample test proposed in this work permitted rapid personnel isolation avoiding further transmission of the virus in the hospital setting&#46;</p><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Funding</span><p id="par0120" class="elsevierStylePara elsevierViewall">This work was partially supported by a grant from <span class="elsevierStyleGrantSponsor" id="gs1">Fondo para la Investigaci&#243;n Cient&#237;fica y Tecnol&#243;gica</span> &#40;FONCYT&#41; &#91;IP-COVID 19&#58; 0938&#93; awarded to Dr&#46; Marcela Echavarr&#237;a and by an internal fund from CEMIC and Fundaci&#243;n &#8220;Norberto Quirno&#8221; &#91;01&#47;20&#93;&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Conflict of interest</span><p id="par0125" class="elsevierStylePara elsevierViewall">MS is a consultant to Basilea&#44; a speaker for Pfizer and the principal investigator in Argentina for NIH grant UM1AI104681&#46; The rest of the authors have no conflict of interest&#46;</p></span></span>"
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            1 => "An&#225;lisis"
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    "highlights" => array:2 [
      "titulo" => "Highlights"
      "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall"><ul class="elsevierStyleList" id="lis0005"><li class="elsevierStyleListItem" id="lsti0005"><span class="elsevierStyleLabel">&#8226;</span><p id="par0005" class="elsevierStylePara elsevierViewall">Saliva screening was helpful for identifying SARS-CoV-2 infection in HCWs&#46;</p></li><li class="elsevierStyleListItem" id="lsti0010"><span class="elsevierStyleLabel">&#8226;</span><p id="par0010" class="elsevierStylePara elsevierViewall">Six percent of asymptomatic&#47;pre-symptomatic HCWs from high&#47;middle area&#44; were positive in saliva&#46;</p></li><li class="elsevierStyleListItem" id="lsti0015"><span class="elsevierStyleLabel">&#8226;</span><p id="par0015" class="elsevierStylePara elsevierViewall">Five out of six HCWs were positive in a subsequent nasopharyngeal swab&#46;</p></li><li class="elsevierStyleListItem" id="lsti0020"><span class="elsevierStyleLabel">&#8226;</span><p id="par0020" class="elsevierStylePara elsevierViewall">Four out of six HCWs developed signs and symptoms compatible with COVID-19&#46;</p></li><li class="elsevierStyleListItem" id="lsti0025"><span class="elsevierStyleLabel">&#8226;</span><p id="par0025" class="elsevierStylePara elsevierViewall">Three out of 4 symptomatic seroconverted while asymptomatic HCWs remained seronegative&#46;</p></li></ul></p></span>"
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        "resumen" => "<span id="abst0010" class="elsevierStyleSection elsevierViewall"><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Health care workers &#40;HCWs&#41; are at high risk for SARS-CoV-2&#46; In addition&#44; pre-symptomatic or asymptomatic transmission accounts for around half of the cases&#46; Saliva testing is an option to detect SARS-CoV-2 infection&#46; To determine the performance of saliva samples for screening&#44; HCWs were tested for SARS-CoV-2 by RT-PCR&#46; Those with a positive result in saliva were tested by nasopharyngeal swabbing for viral RNA detection and blood collection to search for the presence of specific antibodies&#46; In September&#8211;October 2020&#44; 100 HCWs were enrolled and followed up&#46; Six subjects &#40;6&#37;&#41; tested positive in saliva&#46; Of them&#44; 5&#47;6 were positive in a subsequent nasopharyngeal swab and 4&#47;6 developed signs and symptoms compatible with COVID-19&#46; Among the latter&#44; 3 seroconverted while asymptomatic HCWs remained seronegative&#46; Saliva screening was helpful for identifying SARS-CoV-2 infection in HCWs&#46; This screening permitted rapid personnel isolation avoiding further transmission of the virus in the hospital setting&#46;</p></span>"
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        "resumen" => "<span id="abst0015" class="elsevierStyleSection elsevierViewall"><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">El personal de salud &#40;PS&#41; tiene un alto riesgo de contraer SARS-CoV-2&#46; La transmisi&#243;n presintom&#225;tica&#47;asintom&#225;tica representa alrededor de la mitad de los casos y el an&#225;lisis a partir de muestras de saliva puede ser una opci&#243;n para detectar la infecci&#243;n&#46; Para determinar el rendimiento de estas muestras&#44; 100 voluntarios del PS se sometieron a la detecci&#243;n de SARS-CoV-2 por RT-PCR en muestras de saliva en el per&#237;odo septiembre-octubre de 2020&#46; De aquellos con resultado positivo en saliva&#44; se tomaron hisopados nasofar&#237;ngeos para detectar ARN viral y muestras de suero para evaluar anticuerpos espec&#237;ficos&#46; Se detect&#243; ARN viral en la saliva de seis individuos &#40;6&#37;&#41;&#46; De ellos&#44; 5&#47;6 fueron SARS-CoV-2 positivos en hisopado nasofar&#237;ngeo y 4&#47;6 desarrollaron signos y s&#237;ntomas compatibles con COVID-19&#46; Entre estos &#250;ltimos&#44; tres seroconvirtieron&#44; en tanto que los voluntarios asintom&#225;ticos permanecieron seronegativos&#46; La muestra de saliva fue &#250;til para identificar la infecci&#243;n por SARS-CoV-2 en esta cohorte del personal de salud y as&#237; proceder al r&#225;pido aislamiento de los individuos infectados&#44; lo que evit&#243; una mayor transmisi&#243;n del virus en el &#225;mbito hospitalario&#46;</p></span>"
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                  \t\t\t\t" scope="col">ID&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t  " align="center" valign="\n
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                  \t\t\t\t" scope="col">Work area exposure&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">Days after saliva test&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">PCR &#40;Ct&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">High &#40;emergency&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">48&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">POS &#40;36&#46;80&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">10&#47;09&#47;2020&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&#43;1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">POS &#40;29&#46;32&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Myalgias &#40;&#43;4&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">IgM&#58; NegIgG&#58; Neg&#40;&#43;90&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">High &#40;emergency&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">45&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">POS &#40;32&#46;20&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">11&#47;09&#47;2020&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&#43;3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">POS &#40;25&#46;60&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Headache&#44; dyspnea&#44; fever and retroocular pain &#40;&#43;4&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">IgM&#58; PosIgG&#58; Pos&#40;&#43;20&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">High &#40;emergency&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">46&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">M&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">POS &#40;15&#46;26&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">14&#47;09&#47;2020&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&#43;1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">POS &#40;16&#46;96&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Fever&#44; myalgias and headache &#40;&#43;2&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">IgM&#58; PosIgG&#58; Pos&#40;&#43;30&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">High &#40;emergency&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">46&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">POS &#40;35&#46;53&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">15&#47;09&#47;2020&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&#43;1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">NEG&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Asymptomatic&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">IgM&#58; NegIgG&#58; Neg&#40;&#43;34&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">High &#40;COVID hospitalization area&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">42&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">POS &#40;28&#46;19&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">17&#47;09&#47;2020&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&#43;7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">POS &#40;21&#46;45&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Anosmia&#44; dysgeusia&#44; nasal congestion and myalgias &#40;&#43;4&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">IgM&#58; NegIgG&#58; Pos&#40;&#43;33&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">High &#40;COVID hospitalization area&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">49&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">POS &#40;33&#46;71&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">23&#47;09&#47;2020&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">&#43;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">POS &#40;26&#46;81&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Asymptomatic&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">IgM&#58; NegIgG&#58; Neg&#40;&#43;26&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">Health care workers with a positive SARS-CoV-2 PCR in saliva&#46; Clinical follow-up and NPS correlation&#46;</p>"
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0015"
          "bibliografiaReferencia" => array:17 [
            0 => array:3 [
              "identificador" => "bib0090"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Prevalence of positive COVID-19 among asymptomatic health care workers who care patients infected with the novel coronavirus&#58; a retrospective study"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:10 [
                            0 => "N&#46;A&#46; Al-zoubi"
                            1 => "B&#46;R&#46; Obeidat"
                            2 => "M&#46;A&#46; Al-Ghazo"
                            3 => "W&#46;A&#46; Hayajneh"
                            4 => "A&#46;H&#46; Alomari"
                            5 => "T&#46;S&#46; Mazahreh"
                            6 => "I&#46;G&#46; Al-Faouri"
                            7 => "K&#46; Obeidat"
                            8 => "A&#46;B&#46; Issa"
                            9 => "A&#46; Aleshawi"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Ann Med Surg"
                        "fecha" => "2020"
                        "volumen" => "57"
                        "paginaInicial" => "14"
                        "paginaFinal" => "16"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0095"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Occurrence and transmission potential of asymptomatic and presymptomatic SARS-CoV-2 infections&#58; a living systematic review and meta-analysis"
                      "autores" => array:1 [
                        0 => array:2 [
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        "texto" => "<p id="par0130" class="elsevierStylePara elsevierViewall">We are very grateful to all volunteers providing saliva samples and permitting their clinical follow up&#46; We specially thank Dr&#46; Gamarnik and COVIDAR group for providing COVIDAR ELISA kits and for their collaboration&#46; We want to thank Maria Elvina Moussou&#44; Fernando Lopes Mateo and Agostina Nicolelli for their help in sample storage and transportation&#46; We also want to thank technician Victoria Ce&#241;al for helping in serum samples separation&#46;</p>"
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Article information
ISSN: 03257541
Original language: English
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2024 February 55 7 62
2024 January 58 7 65
2023 December 48 11 59
2023 November 133 18 151
2023 October 184 17 201
2023 September 77 10 87
2023 August 59 9 68
2023 July 69 4 73
2023 June 46 12 58
2023 May 176 10 186
2023 April 34 4 38
2023 March 20 11 31
2023 February 24 13 37
2023 January 10 7 17
2022 December 17 20 37
2022 November 18 6 24
2022 October 10 7 17
2022 September 14 18 32
2022 August 11 19 30
2022 July 16 8 24
2022 June 18 20 38
2022 May 4 2 6
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es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos