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Brief report
Characterization of third generation cephalosporin-resistant Escherichia coli clinical isolates from Ushuaia, Argentina
Caracterización de aislamientos clínicos de Escherichia coli resistente a cefalosporinas de tercera generación de Ushuaia, Argentina
Ivan Gramundia, Ezequiel Albornozb, Manuel Boutureiraa, Melina Rapoportb, Sonia Gomezb,c, Alejandra Corsob, Gabriel Castroa, Diego Facconeb,c,
Corresponding author
dfaccone@anlis.gob.ar

Corresponding author.
a Laboratorio de Microbiología, Hospital Regional Ushuaia, Ushuaia, Tierra del Fuego, Argentina
b Servicio Antimicrobianos, Instituto Nacional de Enfermedades Infecciosas-ANLIS “Dr. Carlos G. Malbrán”, Buenos Aires, Argentina
c Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Buenos Aires, Argentina
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coli</span> resistant to &#946;-lactams&#44; particularly to third-generation cephalosporins &#40;TGCs&#41;&#44; represent a major global public health concern&#46; TGCs are used to treat <span class="elsevierStyleItalic">E&#46; coli</span> causing UTIs&#44; bloodstream infections and intra-abdominal infections&#46; In many cases resistance to TGCs is accompanied by resistance to other antimicrobials used for therapy leaving few treatment options&#46; Resistance to TGCs in <span class="elsevierStyleItalic">E&#46; coli</span> can be mediated by different mechanisms&#44; including chromosomally encoded AmpC &#946;-lactamase hyperproduction&#44; increased efflux or reduced outer membrane permeability&#44; however the two main relevant mechanisms are plasmid-mediated extended-spectrum &#946;-lactamases &#40;ESBLs&#41; or plasmid-mediated AmpCs enzymes&#46;<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">13</span></a> There are currently a large number of ESBLs described among <span class="elsevierStyleItalic">E&#46; coli</span> isolates&#44; being CTX-M the most detected family&#46; More than 230 CTX-M variants have been reported&#44; nevertheless CTX-M-2&#44; CTX-M-9&#44; CTX-M-14 and CTX-M-15 are the most frequently described enzymes among <span class="elsevierStyleItalic">Enterobacterales</span><a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">13</span></a>&#40;<a href="https://www.ncbi.nlm.nih.gov/pathogens/refgene/">https&#58;&#47;&#47;www&#46;ncbi&#46;nlm&#46;nih&#46;gov&#47;pathogens&#47;refgene&#47; - ctx-m</a>&#59; last accession 04&#46;19&#46;22&#41;&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">E&#46; coli</span> sequence type 131 &#40;ST131&#41; is a globally disseminated multidrug-resistant &#40;MDR&#41; clone responsible for different human infections including UTI and bloodstream infections&#46;<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">14</span></a> This clone belongs to phylogroup B2 and was successfully spread worldwide&#46; Its main characteristics include the expression of resistance determinants to multiple drugs&#44; including the production of CTX-M-15 ESBL and resistance to fluoroquinolones by chromosomal mutations&#44; and the acquisition of virulence genes&#44; where the possession of the type 1 fimbriae FimH30 allele is common&#46;<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">14</span></a> In addition to <span class="elsevierStyleItalic">E&#46; coli</span> ST131&#44; other relevant ESBL-producing <span class="elsevierStyleItalic">E&#46; coli</span> clones&#44; such as ST10&#44; ST38&#44; ST405&#44; and ST648&#44; were associated with human infections&#46;<a class="elsevierStyleCrossRefs" href="#bib0095"><span class="elsevierStyleSup">2&#44;13</span></a></p><p id="par0045" class="elsevierStylePara elsevierViewall">In Argentina&#44; Marchisio et al&#46; analyzed 71 <span class="elsevierStyleItalic">Enterobacterales</span> recovered from outpatient urine cultures in two health institutions from Santa Fe city during July 2010&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">10</span></a> Among 63 <span class="elsevierStyleItalic">E&#46; coli</span> isolates&#44; only one &#40;1&#46;6&#37;&#41; showed resistance to TGC mediated by CMY-2 plasmidic-AmpC&#46; In another study carried out during October 2010 in 15 hospitals from three regions of Argentina&#44; a total of 1120 <span class="elsevierStyleItalic">E&#46; coli</span> clinical isolates were analyzed&#44; detecting resistance to TGC in 64 strains &#40;5&#46;7&#37;&#41;&#46;<a class="elsevierStyleCrossRef" href="#bib0160"><span class="elsevierStyleSup">15</span></a> Among 13 selected <span class="elsevierStyleItalic">E&#46; coli</span> isolates&#44; 7 harbored <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-15</span>&#44; 3 of them <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-2</span>&#44; and the remaining 3 encoded <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-14</span>&#46;<a class="elsevierStyleCrossRef" href="#bib0160"><span class="elsevierStyleSup">15</span></a> In an additional study performed between November 2012 and April 2013&#44; a total of 3105 community urine samples from adult male patients from a health care institution were analyzed&#46;<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">17</span></a> A total of 374 <span class="elsevierStyleItalic">E&#46; coli</span> isolates were recovered and a frequency of 15&#46;2&#37; of ESBL-producing strains was detected&#44; although no molecular characterization was performed to describe the genes involved&#46;<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">17</span></a> Additionally&#44; according to the WHONET-Argentina network&#44; the national rate of resistance to TGCs among community-onset <span class="elsevierStyleItalic">E&#46; coli</span> infections for the year 2015 was 5&#46;3&#37;&#46;<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">9</span></a></p><p id="par0050" class="elsevierStylePara elsevierViewall">The city of Ushuaia is located in the Isla Grande archipelago&#44; a very important tourist spot&#46; In 2015&#44; Ushuaia had a stable population of &#60;70<span class="elsevierStyleHsp" style=""></span>000 people and received around 400<span class="elsevierStyleHsp" style=""></span>000 national and international tourists &#40;<a href="https://turismoushuaia.com/wp-content/uploads/2018/05/sintesis-2015.pdf">https&#58;&#47;&#47;turismoushuaia&#46;com&#47;wp-content&#47;uploads&#47;2018&#47;05&#47;sintesis-2015&#46;pdf</a>&#41;&#46; A lack of information and studies about the epidemiology of TGC resistance in this city was observed&#46; Therefore<span class="elsevierStyleBold">&#44;</span> the objective of the present work was to characterize and describe TGC-resistant <span class="elsevierStyleItalic">E&#46; coli</span> clinical isolates in the southernmost city of Argentina&#46;</p><p id="par0055" class="elsevierStylePara elsevierViewall">A prospective&#44; descriptive study was performed at the Microbiology Laboratory of Hospital Regional Ushuaia between January 2013 and March 2015&#46; All <span class="elsevierStyleItalic">E&#46; coli</span> isolates were obtained from non-repetitive cultures from inpatients and outpatients&#46; The isolates were recovered from urine&#44; blood&#44; respiratory materials&#44; skin and soft tissue&#44; puncture fluids and prosthetic material&#46; Screening cultures were excluded from the study&#46; Epidemiological data&#44; age and sex were analyzed&#46; Species identification was performed by conventional biochemical methods in accordance to the Manual of Clinical Microbiology&#46; Antimicrobial susceptibility was evaluated by the Kirby-Bauer agar diffusion method&#44; according to CLSI guidelines M100-S23&#46;<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">1</span></a> The antimicrobials tested were defined by the WHONET Argentina Network protocol&#46; Isolates were classified as suspicious of ESBL-production&#44; displaying inhibition halos &#8804;27<span class="elsevierStyleHsp" style=""></span>mm to cefotaxime and&#47;or &#8804;22<span class="elsevierStyleHsp" style=""></span>mm to ceftazidime&#44; and were selected for further characterization&#46; Phenotypic ESBL production was confirmed when there was a positive synergistic effect between discs of amoxicillin-clavulanic acid &#40;10&#47;20<span class="elsevierStyleHsp" style=""></span>&#956;g&#41; and cefotaxime &#40;30<span class="elsevierStyleHsp" style=""></span>&#956;g&#41;&#44; or ceftazidime &#40;30<span class="elsevierStyleHsp" style=""></span>&#956;g&#41;&#46; PCR was performed to detect the following genes&#58; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M</span>&#44; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">PER-2</span>&#44; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">TEM</span>&#44; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">SHV</span> and <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CMY</span>&#46;<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">5</span></a><span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M</span> genes were classified using PCR to differentiate among the following CTX-M groups&#58; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-2</span>&#44; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-1&#47;15</span>&#44; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-8&#47;25</span>&#44; and <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-9&#47;14</span>&#46;<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">5</span></a><span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">TEM</span> gene was amplified and sequenced using the Sanger sequencing method&#46; The presence of clinically relevant <span class="elsevierStyleItalic">E&#46; coli</span> clones from humans was evaluated by multiplex PCR to detect ST69&#44; ST73&#44; ST95&#44; and ST131 clones&#46;<a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">3</span></a> Whole-genome sequencing of selected strains was performed&#44; using DNA extracted with QIAcube&#44; using the QIAamp1 DNA Mini Kit &#40;Qiagen&#41; and sequenced on an Illumina-MiSeq sequencer&#46; Paired-end reads were trimmed with Trim Galore &#40;V&#46;0&#46;6&#46;3&#41; and analyzed for quality with FASTQC &#40;V&#46;0&#46;11&#46;5&#41;&#46; Kraken2 &#40;V&#46;2&#46;0&#46;7-beta&#41; was used to confirm the species&#46; Reads were de novo assembled with Spades ensambler running under Unicycler &#40;v0&#46;4&#46;8-beta&#41; and its quality was evaluated with QUAST &#40;V&#46;5&#46;0&#46;2&#41;&#46; The annotation of the genomes was done with Prokka &#40;V&#46;1&#46;14&#46;0&#41;&#46; ARIBA was run to determine resistance genes &#40;ResFinder&#44; V&#46;2&#46;14&#46;4&#41;&#46; Sequence types &#40;Achtman scheme&#41; for each genome was determined by running ARIBA sequence type &#40;MLST&#44; V&#46;2&#46;14&#46;6&#41; and clonal complexes &#40;CC&#41; were obtained by submitting trimmed reads to Enterobase&#46; The <span class="elsevierStyleItalic">fimH</span> gene&#44; encoding the type 1 fimbriae adhesin&#44; was characterized using fimTyper from the Center of Genomic Epidemiology &#40;<a href="http://www.genomicepidemiology.org/">http&#58;&#47;&#47;www&#46;genomicepidemiology&#46;org&#47;</a>&#41;&#46; The phylogroup assignment was performed in silico according to the Clermont PCR method using the command line tool&#46;<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">16</span></a></p><p id="par0060" class="elsevierStylePara elsevierViewall">During the period from January 2013 to March 2015 a total of 1632 <span class="elsevierStyleItalic">E&#46; coli</span> isolates were recovered at the Hospital Regional de Ushuaia&#44; Tierra del Fuego province&#46; Nearly two percent &#40;32&#47;1632&#59; 1&#46;96&#37;&#41; of the isolates were resistant to TGC and suspected to be ESBL producers&#46; Most suspected isolates were recovered from urine &#40;31&#47;32&#58; 97&#37;&#41;&#44; and one from the peritoneal cavity&#46; The age of the patients ranged from 5 and 84 years&#44; and 87&#37; of them were women&#46; Twenty-seven &#40;84&#37;&#41; isolates were from outpatients while five patients had over 48<span class="elsevierStyleHsp" style=""></span>h of hospitalization at the time of sampling&#46;</p><p id="par0065" class="elsevierStylePara elsevierViewall">Among the 32 <span class="elsevierStyleItalic">E&#46; coli</span> isolates&#44; 91&#37; showed resistance to cefotaxime and 28&#37; to ceftazidime&#46; Twenty-seven &#40;84&#37;&#41; <span class="elsevierStyleItalic">E&#46; coli</span> were phenotypically confirmed as ESBL-producers&#44; therefore the rate of ESBL-producing <span class="elsevierStyleItalic">E&#46; coli</span> from Ushuaia reached 1&#46;65&#37;&#46; The five ESBL-negative <span class="elsevierStyleItalic">E&#46; coli</span> isolates were positive for the plasmid-borne <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CMY</span> gene&#46; Among the 27 ESBL-producer isolates&#44; 26 were positive for <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M</span> gene and one was only positive for <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">TEM</span> gene&#46; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M</span> genes were grouped according to their nucleotide sequence similarity as follows&#58; CTX-M-1&#47;15 &#40;n&#58; 13&#59; 54&#37;&#41;&#59; CTX-M-9&#47;14 &#40;n&#58; 6&#59; 25&#37;&#41;&#59; CTX-M-2 &#40;n&#58; 4&#59; 17&#37;&#41;&#59; and CTX-M-1&#47;15 plus CTX-M-9&#47;14 &#40;n&#58; 1&#59; 4&#37;&#41;&#44; while two isolates were not viable for further studies&#46; The dissociated TGC phenotype&#44; displaying resistance to cefotaxime and susceptibility to ceftazidime&#44; is related to the strong cefotaximase activity of CTX-M family ESBLs&#44; as previously described&#46;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">11</span></a> The TEM-producer <span class="elsevierStyleItalic">E&#46; coli</span> isolate showed a low-level ESBL activity profile and was confirmed by sequencing as ESBL TEM-19&#46; Additional resistance to ampicillin-sulbactam &#40;78&#37;&#41;&#44; ciprofloxacin &#40;75&#37;&#41;&#44; trimethoprim-sulfomethoxazole &#40;65&#37;&#41;&#44; cefoxitin &#40;22&#37;&#41;&#44; and nitrofurantoin &#40;19&#37;&#41; were also observed&#46; No resistance to carbapenems was observed&#46;</p><p id="par0070" class="elsevierStylePara elsevierViewall">Among the 30 available isolates&#44; 12 &#40;40&#37;&#41; were identified as ST131 and one as ST69&#46; Nine out of twelve &#40;75&#37;&#41; <span class="elsevierStyleItalic">E&#46; coli</span> ST131 isolates were positive for the <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-1&#47;15</span> group&#44; one for <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-2</span>&#44; one for <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-9&#47;14</span>&#44; and one for ESBL <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">TEM-19</span>&#46; The ST69 isolate was positive for the <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-2</span> group&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">Considering that the CTX-M-1&#47;15-group was the most prevalent ESBL&#44; seven <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-1&#47;15</span>-positive <span class="elsevierStyleItalic">E&#46; coli</span> isolates &#40;ECO1 to ECO7&#41; were selected for the whole-genome sequencing analysis &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; Four of these strains were confirmed as ST131 &#40;ECO3&#44; ECO4&#44; ECO6 and ECO7&#41;&#44; two isolates &#40;ECO1 and ECO2&#41; were typed as ST617 &#40;clonal complex 10&#41; and one &#40;ECO5&#41; as ST405 &#40;CC405&#41;&#46; The <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-15</span> allele was confirmed by sequencing in all seven isolates&#46; The four ST131-CTX-M-15 producing <span class="elsevierStyleItalic">E&#46; coli</span> isolates harbored additional resistance genes&#58; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">OXA-1</span>&#44; <span class="elsevierStyleItalic">mph&#40;A&#41;</span>&#44; <span class="elsevierStyleItalic">aac&#40;6</span>&#8242;&#41;<span class="elsevierStyleItalic">-Ib-cr</span>&#44; <span class="elsevierStyleItalic">tetA</span>&#44; <span class="elsevierStyleItalic">catB3</span>&#44; <span class="elsevierStyleItalic">sul1</span> and <span class="elsevierStyleItalic">qacE</span> &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; Further resistance genes were found in some of the four ST131 isolates &#40;n&#41;&#58; <span class="elsevierStyleItalic">aadA5</span> &#40;3&#41;&#44; <span class="elsevierStyleItalic">dfrA17</span> &#40;3&#41;&#44; <span class="elsevierStyleItalic">aac&#40;3&#41;-IIa</span> &#40;3&#41;&#44; <span class="elsevierStyleItalic">aadA1</span> &#40;1&#41;&#44; <span class="elsevierStyleItalic">ant&#40;2</span>&#8243;<span class="elsevierStyleItalic">&#41;-Ia</span> &#40;1&#41;&#44; <span class="elsevierStyleItalic">cmlA1</span> &#40;1&#41; &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; All these four ST131-CTX-M-15 producing isolates exhibited the same mutations at the quinolone-resistance determining regions &#40;QRDRs&#41;&#58; Ser83Leu and Asp87Asn of <span class="elsevierStyleItalic">gyrA</span> gene&#44; plus Ser80Ile and Glu84Val of <span class="elsevierStyleItalic">parC</span> gene&#44; and Ile529Leu of <span class="elsevierStyleItalic">parE</span> gene&#46; Additionally&#44; these four ST131 isolates were <span class="elsevierStyleItalic">in silico</span> serotyped as O25&#58;H4 and three of them were typed as fimH30&#44; while the remaining one was 98&#46;65&#37; related to fimH1516 <a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">8</span></a>&#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0080" class="elsevierStylePara elsevierViewall">The two <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-15</span>-producing ST617 <span class="elsevierStyleItalic">E&#46; coli</span> isolates harbored the following acquired resistance genes&#44; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">OXA-1</span>&#44; <span class="elsevierStyleItalic">mph&#40;A&#41;</span>&#44; <span class="elsevierStyleItalic">aac&#40;6</span>&#8242;<span class="elsevierStyleItalic">&#41;-Ib-cr</span>&#44; <span class="elsevierStyleItalic">aadA5</span>&#44; <span class="elsevierStyleItalic">tetB</span>&#44; <span class="elsevierStyleItalic">catB3</span>&#44; <span class="elsevierStyleItalic">dfrA17</span>&#44; <span class="elsevierStyleItalic">sul1</span> and <span class="elsevierStyleItalic">qacE</span> genes&#44; while ECO2 isolate additionally harbored <span class="elsevierStyleItalic">tetA</span>&#44; <span class="elsevierStyleItalic">aac&#40;3&#41;-IIa</span>&#44; <span class="elsevierStyleItalic">aph&#40;3</span>&#8243;<span class="elsevierStyleItalic">&#41;-Ib</span>&#44; <span class="elsevierStyleItalic">aph&#40;6&#41;-Id</span> and <span class="elsevierStyleItalic">sul2</span> genes &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; These isolates showed the following QRDR mutations&#58; Ser83Leu and Asp87Asn of <span class="elsevierStyleItalic">gyrA</span> gene&#44; Ser80Ile of <span class="elsevierStyleItalic">parC</span> gene&#44; and Ser458Ala of <span class="elsevierStyleItalic">parE</span> gene&#46; These two strains&#44; ECO1 and ECO2&#44; were serotyped as O101&#58;H10 but with an untyped <span class="elsevierStyleItalic">fim</span> gene&#46; The ECO5 &#40;ST405&#41; isolate harbored <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-15</span>&#44; <span class="elsevierStyleItalic">mph&#40;A&#41;</span>&#44; <span class="elsevierStyleItalic">tetB</span>&#44; <span class="elsevierStyleItalic">aadA5</span>&#44; <span class="elsevierStyleItalic">aac&#40;3&#41;-IIa</span>&#44; <span class="elsevierStyleItalic">dfrA17</span>&#44; <span class="elsevierStyleItalic">sul1</span> and <span class="elsevierStyleItalic">qacE</span> genes&#44; and showed the same mutations in the QRDR as ST617 strains &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; This strain was serotyped asO102&#58; H6 and showed the fimH27 allele&#46; Additionally&#44; according to the phylogroup analysis&#44; all four <span class="elsevierStyleItalic">E&#46; coli</span> ST131 &#40;CC131&#41; isolates belonged to phylogroup B2&#44; while both ST617 &#40;CC10&#41; belonged to phylogroup A&#44; and <span class="elsevierStyleItalic">E&#46; coli</span> ST405 &#40;CC405&#41; to phylogroup D&#46;</p><p id="par0085" class="elsevierStylePara elsevierViewall">Resistance to TGCs and carbapenems in <span class="elsevierStyleItalic">E&#46; coli</span> is a global public health concern&#44; therefore the prompt detection and characterization of mechanisms involved and the clones associated with the dissemination is essential&#46; Global&#44; regional and local studies are necessary to increase the information about this pathogen&#46; During the period January 2013 to March 2015&#44; 1&#46;96&#37; of <span class="elsevierStyleItalic">E&#46; coli</span> clinical isolates&#44; mainly recovered from urine samples of outpatients in Ushuaia&#44; showed resistance to TGCs&#46; This rate was quite lower than that of other contemporary reports <a class="elsevierStyleCrossRefs" href="#bib0135"><span class="elsevierStyleSup">10&#44;16&#44;17</span></a>and the results of the National surveillance &#40;WHONET-Argentina network&#41; for the year 2015&#44; where the percentage of resistance to TGCs among community-onset <span class="elsevierStyleItalic">E&#46; coli</span> infections was 5&#46;3&#37;&#46;<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">9</span></a></p><p id="par0090" class="elsevierStylePara elsevierViewall">The main mechanism of resistance to TGC was mediated by ESBL production&#44; particularly CTX-M&#46; The CTX-M-1&#47;15 group was the most commonly detected followed by the CTX-M-9&#47;14 and CTX-M-2 groups&#46; The proportion among the CTX-M-groups was similar to a previous report from Argentina&#44; where CTX-M-1&#47;15 enzymes represented &#62;50&#37; of ESBL-producing <span class="elsevierStyleItalic">E&#46; coli</span> isolates&#46;<a class="elsevierStyleCrossRef" href="#bib0160"><span class="elsevierStyleSup">15</span></a> In Ushuaia&#44; urinary tract infections by ESBL-producer organisms are treated with piperacillin-tazobactam or carbapenems&#46;</p><p id="par0095" class="elsevierStylePara elsevierViewall">Plasmid-borne <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CMY</span> gene&#44; which was detected in five ESBL-negative TGC-resistant <span class="elsevierStyleItalic">E&#46; coli</span> isolates&#44; is the second mechanism involved in resistance to TGC&#46; TEM-19 variant&#44; which was also previously described in our country&#44;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">4</span></a> was detected in only one isolate showing marginal ESBL activity&#46; The above information highlights the usefulness of phenotypic assays to confirm even weak ESBL-producers and defines an optimal antimicrobial therapy in infected patients&#46;</p><p id="par0100" class="elsevierStylePara elsevierViewall">There is scarce information from our country about the circulation of the MDR ST131 <span class="elsevierStyleItalic">E&#46; coli</span> clone&#46; The present work analyzes TGC-R <span class="elsevierStyleItalic">E&#46; coli</span> clinical isolates mainly recovered from urine samples and a high proportion &#40;40&#37;&#41; of them were classified as ST131 <span class="elsevierStyleItalic">E&#46; coli</span> clone&#46; A deep analysis by whole-genome sequencing of some of these strains confirmed the main characteristics of this international high-risk clone&#44; such as the MDR phenotype&#44; including resistance to fluoroquinolones and TGC&#44; serotype O25&#58;H4&#44; fimbriae FimH30&#44; and grouping into the phylogroup B2&#46; This information confirms the circulation of this international clone in Ushuaia and contributes to a better understanding of its spread throughout our country&#46; Similarly&#44; the finding of the other two ESBL-producing international high-risk clones&#44; ST617 &#40;CC10&#41; and ST405&#44; highlights a concerning scenario&#46;<a class="elsevierStyleCrossRefs" href="#bib0115"><span class="elsevierStyleSup">6&#44;13</span></a></p><p id="par0105" class="elsevierStylePara elsevierViewall">The present study was conducted in Ushuaia&#44; the southernmost city of Argentina located in the Isla Grande archipelago and could forecast a different behavior of antimicrobial resistance dissemination compared to continental cities&#46; The following were some limitations of our work&#58; &#40;i&#41; it was not possible to analyze if patients were residents or visitors to Ushuaia city&#44; which could have an important effect on the incidence of antimicrobial resistance&#59; ii&#41; the number of fully sequenced isolates was limited&#59; and iii&#41; a complete characterization of <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M</span>-carrying plasmids was not performed&#46;</p><p id="par0110" class="elsevierStylePara elsevierViewall">Nevertheless&#44; the information presented in this manuscript contributes to a better understanding of the dissemination of antimicrobial resistance among <span class="elsevierStyleItalic">E&#46; coli</span> in our country&#44; and alerts about the spread of <span class="elsevierStyleItalic">E&#46; coli</span> ST131&#44; ST617 and ST405 pandemic clones even in remote cities&#46;</p><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Conflict of interest</span><p id="par0115" class="elsevierStylePara elsevierViewall">The authors declare that they have no conflicts of interest&#46;</p></span></span>"
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      "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall"><ul class="elsevierStyleList" id="lis0005"><li class="elsevierStyleListItem" id="lsti0005"><span class="elsevierStyleLabel">&#8226;</span><p id="par0005" class="elsevierStylePara elsevierViewall">Characterization of third generation cephalosporin-resistant <span class="elsevierStyleItalic">Escherichia coli</span> clinical isolates from Ushuaia&#44; Argentina&#46;</p></li><li class="elsevierStyleListItem" id="lsti0010"><span class="elsevierStyleLabel">&#8226;</span><p id="par0010" class="elsevierStylePara elsevierViewall">32 third generation cephalosporin-resistant <span class="elsevierStyleItalic">E&#46; coli</span> from Ushuaia&#44; Argentina were studied&#46;</p></li><li class="elsevierStyleListItem" id="lsti0015"><span class="elsevierStyleLabel">&#8226;</span><p id="par0015" class="elsevierStylePara elsevierViewall">International high-risk clones ST131&#44; ST617 and ST405 were detected&#46;</p></li><li class="elsevierStyleListItem" id="lsti0020"><span class="elsevierStyleLabel">&#8226;</span><p id="par0020" class="elsevierStylePara elsevierViewall">CTX-M ESBL was the main mechanism of resistance to third generation cephalosporins&#46;</p></li><li class="elsevierStyleListItem" id="lsti0025"><span class="elsevierStyleLabel">&#8226;</span><p id="par0025" class="elsevierStylePara elsevierViewall">Whole genome sequence analysis of 7 MDR <span class="elsevierStyleItalic">E&#46; coli</span> allowed full characterization of these isolates&#46;</p></li></ul></p></span>"
    ]
    "resumen" => array:2 [
      "en" => array:2 [
        "titulo" => "Abstract"
        "resumen" => "<span id="abst0010" class="elsevierStyleSection elsevierViewall"><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">Escherichia coli</span> is one of the main human pathogens causing different hospital- and community-acquired infections&#46; During the period from January 2013 to March 2015&#44; 1&#46;96&#37; &#40;32&#47;1632&#41; of <span class="elsevierStyleItalic">E&#46; coli</span> isolates recovered at the Hospital Regional de Ushuaia&#44; Tierra del Fuego province&#44; were resistant to third-generation cephalosporins &#40;TGCs&#41;&#46; These isolates were resistant to cefotaxime &#40;91&#37;&#41; and&#47;or ceftazidime &#40;28&#37;&#41;&#46; No resistance to carbapenems was detected&#46; Twenty-six isolates were positive for <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M</span> gene&#44; grouped as CTX-M-1&#47;15 &#40;54&#37;&#41;&#59; CTX-M-9&#47;14 &#40;25&#37;&#41;&#59; CTX-M-2 &#40;17&#37;&#41;&#59; and CTX-M-1&#47;15 plus CTX-M-9&#47;14 &#40;4&#37;&#41;&#46; Five TGC-resistant strains were positive for <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CMY</span> gene&#44; while one strain harbored TEM-19 ESBL&#46; Twelve isolates were identified as ST131 <span class="elsevierStyleItalic">E&#46; coli</span> hyperepidemic clone&#44; and one as ST69&#46; Genome sequence analysis of seven <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-15</span><span class="elsevierStyleItalic">E&#46; coli</span> selected isolates confirm the circulation of ST131&#44; ST617 and ST405 international high-risk clones in the city of Ushuaia&#46;</p></span>"
      ]
      "es" => array:2 [
        "titulo" => "Resumen"
        "resumen" => "<span id="abst0015" class="elsevierStyleSection elsevierViewall"><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">Escherichia coli</span> es uno de los principales pat&#243;genos humanos causantes de diferentes infecciones de inicio hospitalario y comunitario&#46; Se determin&#243; que el 1&#44;96&#37; &#40;32&#47;1&#46;632&#41; de los aislamientos de <span class="elsevierStyleItalic">E&#46; coli</span> recuperados entre enero de 2013 y marzo de 2015 en el Hospital Regional de Ushuaia&#44; provincia de Tierra del Fuego&#44; fueron resistentes a cefalosporinas de tercera generaci&#243;n &#40;CTG&#41;&#46; Estos aislamientos fueron resistentes a cefotaxima &#40;91&#37;&#41; y&#47;o a ceftazidima &#40;28&#37;&#41;&#46; No se detect&#243; resistencia a los carbapenemes&#46; Veintis&#233;is aislamientos fueron positivos para el gen <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M</span>&#44; agrupados como CTX-M-1&#47;15 &#40;54&#37;&#41;&#44; CTX-M-9&#47;14 &#40;25&#37;&#41;&#44; CTX-M-2 &#40;17&#37;&#41; y CTX-M-1&#47;15 m&#225;s CTX-M-9&#47;14 &#40;4&#37;&#41;&#46; Cinco cepas resistentes a CTG dieron positivo para el gen <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CMY</span>&#44; mientras que un aislamiento present&#243; la BLEE TEM-19&#46; Doce aislamientos se identificaron como clon hiperepid&#233;mico <span class="elsevierStyleItalic">E&#46; coli</span> ST131 y uno como ST69&#46; El an&#225;lisis de las secuencias del genoma de siete aislamientos seleccionados de <span class="elsevierStyleItalic">E&#46; coli</span><span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-15</span> confirm&#243; la circulaci&#243;n de los clones internacionales de alto riesgo ST131&#44; ST617 y ST405 en la ciudad de Ushuaia&#46;</p></span>"
      ]
    ]
    "multimedia" => array:1 [
      0 => array:8 [
        "identificador" => "tbl0005"
        "etiqueta" => "Table 1"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "detalles" => array:1 [
          0 => array:3 [
            "identificador" => "at1"
            "detalle" => "Table "
            "rol" => "short"
          ]
        ]
        "tabla" => array:2 [
          "leyenda" => "<p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">AMP&#58; ampicillin&#59; AMC&#58; amoxicillin-clavulanate&#59; FGC&#58; first generation cephalosporins&#59; TGC&#58; third generation cephalosporins&#59; FOX&#58; cefoxitin&#59; PTZ&#58; piperacillin-tazobactam&#59; FEP&#58; cefepime&#59; CIP&#58; ciprofloxacin&#59; TMS&#58; trimethoprim-sulfamethoxazole&#59; AKN&#58; amikacin&#59; GEN&#58; gentamicin&#59; TET&#58; tetracycline&#46; NA&#58; not available&#46;</p>"
          "tablatextoimagen" => array:1 [
            0 => array:1 [
              "tabla" => array:1 [
                0 => """
                  <table border="0" frame="\n
                  \t\t\t\t\tvoid\n
                  \t\t\t\t" class=""><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Strain&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ECO1&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ECO2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ECO5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ECO3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ECO4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ECO6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ECO7&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Age &#40;years&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">70&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">39&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">62&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">25&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">55&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">62&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">30&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Sex&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">M&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">M&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">F&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Sample&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Urine&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Urine&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Urine&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Urine&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Urine&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Urine&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Urine&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Community&#47;Hospital onset&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Community&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Community&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Community&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Community&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Community&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Hospital&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Community&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Antimicrobial resistance profile&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">AMP&#44; FGC&#44; TGC&#44; FOX&#44; CIP&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">AMP&#44; AMC&#44; FGC&#44; TGC&#44; PTZ&#44; FEP&#44; CIP&#44; TMS&#44; AKN&#44; GEN&#44; TET&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">AMP&#44; FGC&#44; TGC&#44; FEP&#44; FOX&#44; CIP&#44; TMS&#44; TET&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">AMP&#44; FGC&#44; TGC&#44; FEP&#44; CIP&#44; TMS&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">AMP&#44; FGC&#44; TGC&#44; PTZ&#44; FEP&#44; CIP&#44; TMS&#44; AKN&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">AMP&#44; FGC&#44; TGC&#44; PTZ&#44; FEP&#44; CIP&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">AMP&#44; FGC&#44; TGC&#44; FEP&#44; CIP&#44; TMS&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Sample date&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">4-15-14&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">2-4-15&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">10-1-14&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">7-19-13&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">NA&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">11-20-14&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">3-16-15&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Sequence type &#40;CC&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ST617 &#40;CC10&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ST617&#40;CC10&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ST405 &#40;CC405&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ST131 &#40;CC131&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ST131 &#40;CC131&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ST131 &#40;CC131&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">ST131 &#40;CC131&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Acquired resistance genes&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t"><span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-15</span>&#44; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">OXA-1</span>&#44; <span class="elsevierStyleItalic">mdf&#40;A&#41;&#44; mph&#40;A&#41;&#44; tetB&#44; aac&#40;6</span>&#8242;<span class="elsevierStyleItalic">&#41;-Ib-cr&#44; aadA5&#44; dfrA17&#44; catB3&#44; qacE&#44; sul1</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t"><span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-15</span>&#44; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">OXA-1</span>&#44; <span class="elsevierStyleItalic">mdf&#40;A&#41;&#44; mph&#40;A&#41;&#44; tetB&#44; tetA&#44; aac&#40;6</span>&#8242;<span class="elsevierStyleItalic">&#41;-Ib-cr&#44; aadA5&#44; aac&#40;3&#41;-IIa&#44; aph&#40;3</span>&#8243;<span class="elsevierStyleItalic">&#41;-Ib&#44; aph&#40;6&#41;-Id&#44; dfrA17&#44; catB3&#44; qacE&#44; sul1&#44; sul2</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t"><span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-15</span>&#44; <span class="elsevierStyleItalic">mdf&#40;A&#41;&#44; mph&#40;A&#41;&#44; tetB&#44; aadA5&#44; aac&#40;3&#41;-IIa&#44; dfrA17&#44; qacE&#44; sul1</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t"><span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-15</span>&#44; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">OXA-1</span>&#44; <span class="elsevierStyleItalic">mdf&#40;A&#41;&#44; mph&#40;A&#41;&#44; tetA&#44; aac&#40;6</span>&#8242;<span class="elsevierStyleItalic">&#41;-Ib-cr&#44; aadA5&#44; catB3&#44; dfrA17&#44; qacE&#44; sul1</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t"><span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-15</span>&#44; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">OXA-1</span>&#44; <span class="elsevierStyleItalic">mdf&#40;A&#41;&#44; mph&#40;A&#41;&#44; tetA&#44; aac&#40;6</span>&#8242;<span class="elsevierStyleItalic">&#41;-Ib-cr&#44; aadA5&#44; aac&#40;3&#41;-IIa&#44; catB3&#44; dfrA17&#44; qacE&#44; sul1</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t"><span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-15</span>&#44; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">OXA-1</span>&#44; <span class="elsevierStyleItalic">mdf&#40;A&#41;&#44; mph&#40;A&#41;&#44; tetA&#44; aac&#40;6</span>&#8242;<span class="elsevierStyleItalic">&#41;-Ib-cr&#44; aadA1&#44; aac&#40;3&#41;-IIa&#44; ant&#40;2</span>&#8243;<span class="elsevierStyleItalic">&#41;-Ia&#44; catB3&#44; sul1&#44; qacE&#44; cmlA1</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t"><span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">CTX-M-15</span>&#44; <span class="elsevierStyleItalic">bla</span><span class="elsevierStyleInf">OXA-1</span>&#44; <span class="elsevierStyleItalic">mdf&#40;A&#41;&#44; mph&#40;A&#41;&#44; tetA&#44; aac&#40;6</span>&#8242;<span class="elsevierStyleItalic">&#41;-Ib-cr&#44; aadA5&#44; aac&#40;3&#41;-IIa&#44; dfrA17&#44; catB3&#44; qacE&#44; sul1</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Chromosomal mutations associated with fluoroquinolone resistance&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">gyrA &#40;S83L&#59; D87N&#41;&#44; parC &#40;S80I&#41;&#44; parE &#40;S458A&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">gyrA &#40;S83L&#59; D87N&#41;&#44; parC &#40;S80I&#41;&#44; parE &#40;S458A&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">gyrA &#40;S83L&#59; D87N&#41;&#44; parC &#40;S80I&#41;&#44; parE &#40;S458A&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">gyrA &#40;S83L&#59; D87N&#41;&#44; parC &#40;S80I&#59; E84V&#41;&#44; parE &#40;I529L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">gyrA &#40;S83L&#59; D87N&#41;&#44; parC &#40;S80I&#59; E84V&#41;&#44; parE &#40;I529L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">gyrA &#40;S83L&#59; D87N&#41;&#44; parC &#40;S80I&#59; E84V&#41;&#44; parE &#40;I529L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">gyrA &#40;S83L&#59; D87N&#41;&#44; parC &#40;S80I&#59; E84V&#41;&#44; parE &#40;I529L&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Plasmidic incompatibility groups&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Col&#40;MG828&#41;&#44; IncFIA&#44; IncFIB&#44; IncFII&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Col156&#44; IncFIA&#44; IncFIB&#44; IncFII&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Col156&#44; IncFIB&#44; IncFII&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Col&#40;MG828&#41;&#44; Col156&#44; IncFIA&#44; IncFII&#44; IncY&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Col&#40;MG828&#41;&#44; IncFIA&#44; IncFII&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Col&#40;MG828&#41;&#44; Col156&#44; IncFIA&#44; IncFII&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">IncFIA&#44; IncFIB&#44; IncFII&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">SerotypeFinder&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">O101&#44; H10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">O101&#44; H10&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">O102&#44; H6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">O25&#44; H4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">O25&#44; H4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">O25&#44; H4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">O25&#44; H4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t ; entry_with_role_rowhead " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">fimTyper&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Unknown&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">Unknown&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">fimH27&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">fimH30&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">fimH1516-like&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">fimH30&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="\n
                  \t\t\t\t\ttop\n
                  \t\t\t\t">fimH30&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
            ]
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">Epidemiological and genomic results of five selected TGC-resistant <span class="elsevierStyleItalic">E&#46; coli</span> isolates&#46;</p>"
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0015"
          "bibliografiaReferencia" => array:17 [
            0 => array:3 [
              "identificador" => "bib0090"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Performance standards for antimicrobial susceptibility testing&#59; 25th informational supplement&#44; M100-S25&#44; Wayne&#44; PA&#44; EE&#46;UU&#46;"
                      "autores" => array:1 [
                        0 => array:2 [
                          "colaboracion" => "Clinical and Laboratory Standards Institute"
                          "etal" => false
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Libro" => array:1 [
                        "fecha" => "2015"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0095"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Extended-spectrum &#946;-lactamase-producing <span class="elsevierStyleItalic">Escherichia coli</span> in human-derived and foodchain-derived samples from England&#44; Wales&#44; and Scotland&#58; an epidemiological surveillance and typing study"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:13 [
                            0 => "M&#46;J&#46; Day"
                            1 => "K&#46;L&#46; Hopkins"
                            2 => "D&#46;W&#46; Wareham"
                            3 => "M&#46;A&#46; Toleman"
                            4 => "N&#46; Elviss"
                            5 => "L&#46; Randall"
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Article information
ISSN: 03257541
Original language: English
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es en pt

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Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos