Actividad de la telomerasa e infección por VPH en raspados cervicales de mujeres que desarrollaron lesiones escamosas intraepiteliales de alto grado. Un estudio de seguimiento

Article information

Resumen

Objetivo

Describir la AT y la infeccion por VPH en el seguimiento de mujeres que pertenecen a la cohorte de Bogota.

Métodos

Se analizaron 79 muestras del seguimiento de 25 mujeres que desarrollaron LEI-AG y 149 muestras del seguimiento de 34 mujeres con citologia normal. La deteccion del VPH se realizo usando PCR-EIA GP5+/GP6+ y RLB. La AT se midio mediante TRAP-ELISA.

Resultados

El analisis mostro que de los 25 casos, 8 fueron casos prevalentes (ingresaron al estudio con la LEI-AG) y los 17 casos restantes fueron incidentes (la lesion se detecto durante el seguimiento). De estas 17 mujeres, 12 (70,5%) presentaron AT y VPH al momento del diagnostico o en una visita previa, con VPH de alto riesgo (VPH-AR), principalmente de la especie α-9. Tres mujeres (17,7%) mostraron infecciones transitorias por VPH y 2 (11,8%) no tuvieron VPH o AT al diagnostico. El seguimiento de la mujeres con citologia normal mostro que solo ocho mujeres tuvieron VPH y AT al mismo tiempo (23,5%), 21/34 mujeres (61,8%) tuvieron eventos transitorios de VPH durante el seguimiento y 5 (14,7%) no tuvieron VPH durante todo el seguimiento.

Conclusiones

Detectar AT e infeccion por VPH-AR al mismo tiempo parecen predecir el riesgo de LEI-AG.

Palabras clave:

virus del papiloma humano

telomerasa

cancer de cuello uterino

estudios de seguimiento

Abstract

Objective

To describe telomerase activity (TA) and HPV infection in follow up of women in the Bogota cohort.

Methods

Analysis was carried out on 79 follow up samples from 25 women who developed LEI-AG, and 149 follow up samples from 34 women with normal cytology. HPV detection was made with PCR-EIA GP5+/GP6+ and RLB. TA was measured with TRAP-ELISA.

Results

Analysis revealed that out of the 25 cases, 8 were prevalent (enrolled in the study with LEI-AG), and the remaining 17 incidental (lesion was detected during follow up). Among these 17 women, 12 (70.5%) had, at diagnosis or during a previous checkup, TA and high-risk HPV (HPV-AR), primarily type α-9. Three women (17.7%) had transitory HPV infections, and 2 (11.8%) had neither HPV nor TA at diagnosis. Follow up on women with normal cytology revealed that only eight women (23.5%) had HPV and TA at the same time, 21/34 women (61.8%) had transitory HPV event during follow up, and 5 (14.7%) had no HPV during entirety of follow up.

Conclusions

Detection of TA and simultaneous HPV-AR infection apparently predicts LEI-AR risk.

Key words:

Human papillomavirus

telomerase

cervical cancer

follow-up studies

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