Información del artículo
:Como parte del programa de investigación en retinopatía diabética, y utilizando el humor vítreo bovino como material de partida, nuestro grupo ha aislado y caracterizado un nuevo lípido complejo con potente actividad movilizadora de calcio intracelular, no relacionado con ninguno de los factores de crecimiento descritos hasta este momento en el humor vítreo. Este factor lipídico induce movilización de calcio de depósitos intracelulares sensibles a ácido fosfatídico pero no sensibles a D-mio-inositol-1,4,5- trifosfato. Se describe, por una parte, la implicación de factores intrínsecos bioactivos de naturaleza lipídica y, por otra, la activación de “nuevas” rutas de señalización intracelular. La identificación de este tipo de lípidos, con importantes propiedades a escala celular, podría abrir nuevas puertas en el entendimiento de la fisiopatología en el ojo.
Palabras clave:
Retinopatía diabética
Lípidos
Factores de crecimiento
:We have characterized a lipoid agent which is a potent intracelular calcium activater not related to other growth factors in vitreous humor. This agent movilises intracelular calcium sensitive to fosfatidic acid but not sensitive to D-mio-inositol- 1,4,5 triphosphate.
:We describe lipoid bioactive intrinsic factors as new activators of new pathways.
Key words:
Diabetic retinopathy
Lipids
Growth factors
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Biblografía
[1.]
P. Barry.
The management of diabetic disease.
Textbook of diabetes (2.a ed.). Vol 2, pp. 47.1-47.18
[2.]
P. Wiedemann.
Growth factors in retinal diseases: proliferative vitreoretinopathy, proliferative diabetic retinopathy, and retinal degeneration.
Surv Ophthalmol, 36 (1992), pp. 373-384
[3.]
A. Baird, F. Culler, K. Jones.
Angiogenic factor in human ocular fluid.
Lancet, 2 (1985), pp. 563
[4.]
A. Silvalingham, J. Kenney, G. Brown, W. Benson, L. Donoso.
Basic fibroblast growth factor levels in vitreous of patients with proliferative diabetic retinopathy.
Arch Ophthalmol, 108 (1990), pp. 869-872
[5.]
A. Adamis, J. Miller, M. Bernal, D. D'Amico, J. Folkman, T. Yeo, et al.
Increased vascular endothelial growth factor levels in the vitreous of eyes with proliferative diabetic retinopathy.
Am J Ophthalmol, 118 (1994), pp. 445-450
[6.]
L. Aiello, R. Avery, P. Arrigg, B. Keyt, H. Jampel, S. Shah, et al.
Vascular endothelial growth factor in ocular fluid of patients with diabetic retinopathy and other retinal disorders.
N Engl J Med, 331 (1994), pp. 1480-1487
[7.]
R. Meyer Schwickerath, A. Pfeiffer, W. Blum, H. Freyberger, M. Klein, C. Losche, et al.
Vitreous levels of the insulin-like growth factors I and II and the insulin-like growth factor binding proteins 2 and 3, increase in neovascular eye disease. Studies in nondiabetic and diabetic subjects.
J Clin Invest, 92 (1993), pp. 2620-2625
[8.]
C. Hardwick, R. Feist, R. Morris, M. White, D. Witherspoon, R. Angus, et al.
Tractional force generation by porcine Muller cells: stimulation by growth factors in human vitreous.
Invest Ophthalmol Vis Sci, 38 (1997), pp. 2053-2063
[9.]
J. Luna, C. Chan, N. Derevjanik, J. Mahlow, C. Chiu, B. Peng, et al.
Bloodretinal barrier (BRB) breakdown in experimental autoimmune uveoretinitis: comparison with vascular endothelial growth factor, tumor necrosis factor alpha, and interleukin-1beta-mediated breakdown.
J Neurosci Res, 49 (1997), pp. 268-280
[10.]
A. Canton, R. Burgos, C. Hernandez, C. Mateo, R. Segura, J. Mesa, et al.
Hepatocyte growth factor in vitreous and serum from patients with proliferative diabetic retinopathy.
Br J Ophthalmol, 84 (2000), pp. 732-735
[11.]
S. Tang, O. Scheiffarth, S. Thurau, G. Wildner.
Cells of immmune system and their cytokines in epiretinl membranes and in the vitreous of patients with proliferative diabetic retinopathy.
Ophthalmic Res, 25 (1993), pp. 177-185
[12.]
A. Asrar, D. Maimone, P. Morse, S. Gregory, A. Reder.
Cytokines in the vitreous of patients with proliferative diabetic retinopathy.
Am J Ophthalmol, 114 (1992), pp. 731-736
[13.]
M. Boulton, Z. Gregor, D. McLeod, D. Charteris, J. Jarvis Evans, P. Moriarty, et al.
Intravitreal growth factors in proliferative diabetic retinopathy: correlation with neovascular activity and glycaemic management.
Br J Ophthalmol, 81 (1997), pp. 228-233
[14.]
M. Boulton.
A role for hepatocyte growth factor in diabetic retinopathy?.
Br J Ophthalmol, 83 (1999), pp. 763-764
[15.]
G.S. Schultz, M.B. Grant.
Neovascular growth factors.
Eye, 5 (1991), pp. 170-180
[16.]
A. Pfeiffer, J. Spranger, R. Meyer-Schwickerath, H. Schatz.
Growth factor alterations in advanced diabetic retinopathy. A possible role of blood retina barrier breakdown.
Diabetes, 46 (1997), pp. S26-S30
[17.]
W. Moolenaar.
Development of our current understanding of bioactive lysophospholipids.
Ann N Y Acad Sci, 905 (2000), pp. 1-10
[18.]
W. Moolenaar.
Lysiphosphatidic acid signalling.
Curr Opin Cell Biol, 7 (1995), pp. 203-210
[19.]
W. Moolenaar, O. Kranenburg, F. Postma, C. Zondag.
LPA: G-protein signalling and cellular responses.
Curr Opin Cell Biol, 9 (1997), pp. 168-173
[20.]
A. Merrill, E. Schmelz, D. Dillehay, S. Spiegel, J. Shayman, J. Schroeder, et al.
Sphingolipids.
Toxicol Appl Pharmacol, 142 (1997), pp. 208-225
[21.]
S. Spigel.
Sphingosine-1-phosphate: a ligand for the EDG-1 family of G-protein-coupled receptors.
Ann N Y Acad Sci, 905 (2000), pp. 54-60
[22.]
S. Spiegel, S. Milstien.
Sphingosine-1-phosphate: signaling inside and out.
FEBS Lett, 476 (2000), pp. 55-57
[23.]
S. Orlati, A. Porcelli, S. Hrelia, A. Lorenzini, M. Rugolo.
Intracellular calcium mobilization and phospholipid degradation in sphingosylphosphorylcholine-stimulated human airway epithelial cells.
Biochem J, 334 (1998), pp. 641-649
[24.]
C. Pombo, L. Bokser, X. Casabiell, J. Zugaza, M. Capeans, M. Salorio, et al.
Partial characterization of a putative new growth factor present in pathological human vitreous.
Graefe's Arch Clin Exp Ophthalmol, 234 (1996), pp. 155-163
[25.]
J. Camiña, X. Casabiell, F. Pérez, M. Lage, F. Casanueva.
Isolation of a bioactive Ca2+ -mobilizing complex lipid from bovine vitreous body.
Bichem Biophys Res Commun, 244 (1998), pp. 696-700
[26.]
J. Camiña, X. Casabiell, F. Casanueva.
Ins(1,4,5)P3-independent Ca2+ mobilization triggered by a lipid factor isolated from vitreous body.
J Biol Chem, 274 (1999), pp. 28134-28141
[27.]
J. Camiña, F. Casanueva.
Vitreous-derived lipid factor: a new eye related growth factor?.
Recent Res Devel Endocrinol, 1 (2000), pp. 145-154
[28.]
D. Caruelle, B. Groux Muscatelli, A. Gaudric, C. Sestier, G. Coscas, J. Caruelle, et al.
Immunological study of acidic fibroblast growth factor (aFGF) distribution in the eye.
J Cell Biochem, 39 (1989), pp. 117-128
[29.]
G. Neufeld, D. Gospodarowicz.
Basic and acidic fibroblast growth factors interact with the same cell surface receptors.
J Biol Chem, 261 (1986), pp. 5631-5635
[30.]
M. Berridge.
Inositol triphosphate and diacylglycerol: two interacting second messengers.
Annu Rev Biochem, 56 (1987), pp. 159-193
[31.]
M. Berridge.
Inositol triphsophate and calcium signalling.
Londres: Nature,, 361 (1993), pp. 315-326
[32.]
W. Moolenaar.
Bioactive lysophospholipids and their G protein-coupled receptors.
Exp Cell Res, 253 (1999), pp. 230-238
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