Originales
Monitorización de la enfermedad mínima residual mediante detección conjunta de los reordenamientos PML/RARα y RARα/PML en la leucemia promielocítica aguda
Monitoring of minimal residual disease by combined detection of PML/RARα and RARα/PML rearrangements in acute promyelocytic leukemia
Pascual Bolufer,a,
, Eva Barragána, Miguel Ángel Sanzb, Guillermo Martínb, Enrique Lermaa, Esperanza Afán de Riberaa
Autor para correspondencia
boluferpas@gva.es
Correspondencia: Dr. P. Bolufer Gilabert. Laboratorio de Biología Molecular (Laboratorio de Hormonas). Centro Maternal. Hospital Universitario La Fe. Avda. Campanar, 21. 46009 Valencia
Correspondencia: Dr. P. Bolufer Gilabert. Laboratorio de Biología Molecular (Laboratorio de Hormonas). Centro Maternal. Hospital Universitario La Fe. Avda. Campanar, 21. 46009 Valencia
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Departamento de Biopatología Clínica</span>" "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff00005" ] 1 => array:3 [ "entidad" => "<span class="elsevierStyleItalic">Sección de Hematología Clínica. Servicio de Hematología y Hemoterapia. Hospital Universitario La Fe. Valencia</span>" "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff00010" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "*" "correspondencia" => "Correspondencia: Dr. P. Bolufer Gilabert. Laboratorio de Biología Molecular (Laboratorio de Hormonas). Centro Maternal. Hospital Universitario La Fe. Avda. Campanar, 21. 46009 Valencia" ] ] ] ] "titulosAlternativos" => array:1 [ "en" => array:1 [ "titulo" => "Monitoring of minimal residual disease by combined detection of PML/RARα and RARα/PML rearrangements in acute promyelocytic leukemia" ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "1999-08-27" "fechaAceptado" => "2000-01-20" "PalabrasClave" => array:1 [ "es" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec221300" "palabras" => array:5 [ 0 => "RARa/PML" 1 => "PML/RARa" 2 => "Leucemia promielocítica aguda" 3 => "Monitorización" 4 => "Enfermedad mínima residual" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "es" => array:1 [ "resumen" => "<span class="elsevierStyleSectionTitle">Fundamento</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">: La ajustada sensibilidad del metodo de RT-PCR empleado en la deteccion del reordenamientoPML/RARα en la leucemia promielocitica aguda (LPA), en comparacion con lamayor sensibilidad alcanzada para otros reordenamientos, justifico que en el presente estudiose planteara la monitorizacion de la enfermedad minima residual (EMR) en la LPA determinandoconjuntamente el reordenamiento RARα y el RARα/PML.</p> <span class="elsevierStyleSectionTitle">Pacientes Y Métodos</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">: Se incluyeron 56 pacientes con LPA tratados siguiendo el protocolo PETHEMALPA-96. La deteccion de PML/RARα se efectuo segun el metodo de Biondi et al y la deRARα/PML siguiendo el procedimiento de Grimwade D et al (Human Press Inc.).</p> <span class="elsevierStyleSectionTitle">Resultados</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">: RARα/PML se detecto en el 90% (20/22) de los pacientes al diagnostico positivopara PML/RARα. En la postinduccion, RARα/PML se detecto en el 74% de los pacientes(14/19) frente al 37% (7/19) de PML/RARα. Asimismo, en algunas muestras en la posconsolidacion(2/11) se detecto RARα/PML, siendo negativas para PML/RARα. En los pacientes enmantenimiento se detecto una mayor proporcion de positividades (6/28) para RARα/PML quepara PML/RARα (2/28). En un paciente en remision completa, RARα/PML precedio a la apariciondel PML/RARα y persistio un mayor tiempo tras la negativizacion de PML/RARα. En elgrupo de pacientes seguidos desde el diagnostico se observo que RARα/PML suele negativizarseun mes despues de la negativizacion de PML/RARα.</p> <span class="elsevierStyleSectionTitle">Conclusiones</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">: RARα/PML se expresa en el 90% de los pacientes con LPA al diagnostico.RARα/PML se detecta en una mayor proporcion de muestras recogidas durante la evolucion quePML/RARα. RARα/PML persiste un mayor tiempo que PML/RARα tras el tratamiento inicial.</p>" ] "en" => array:1 [ "resumen" => "<span class="elsevierStyleSectionTitle">Background</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">: Molecular assay commonly used to detect the PML/RARá rearrangement in acutepromyelocytic leukemia (APL) has the limited sensitivity in comparison with the higher sensitivityof RARá/PML detection. This prompted us to perform both assays in parallel to monitor agroup of APL.</p> <span class="elsevierStyleSectionTitle">Patients and Methods</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">: The study included 56 APL patients mainly treated according with thePETHEMA LPA-96 protocol. The PML/RARá was detected according with Biondi's et al methodand the RARá/PML following the Grimwade's et al RT-PCR method (Human Press Inc.).</p> <span class="elsevierStyleSectionTitle">Results</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">: RARá/PML rearrangement was detected in 90% (20/22) of the patients at diagnosis positivesfor PML/RARá. RARá/PML was detected in 74%(14/19) of post-induction samples versus37% (7/19) of positives for PML/RARá. Likewise RARá/PML rearrangement was detected in somepost-consolidation samples (2/11) that all were PMI/RARá negatives. In patients in maintenanceregimen a greater proportion of RARá/PML positives (6/28) versus PML/RARá (2/28) were observed.In a patient in complete remission RARá/PML preceded the positivity of PML/RARá and persistedafter PMI/RARá negativization. The results of the patients monitored since the diagnosisshowed that RARá/PML revert to negative one month after PML/RARá negativization.</p> <span class="elsevierStyleSectionTitle">Conclusions</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">: RARá/PML rearrangement is not expressed in the totality of the APL patients, butin only a 90% of them. RARá/PML rearrangement was detected in a greater proportion of samplesthan PML/RARá. 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| año/Mes | Html | Total | |
|---|---|---|---|
| 2024 Noviembre | 0 | 2 | 2 |
| 2024 Octubre | 0 | 27 | 27 |
| 2024 Septiembre | 0 | 31 | 31 |
| 2024 Agosto | 0 | 12 | 12 |
| 2024 Julio | 0 | 20 | 20 |
| 2024 Junio | 0 | 24 | 24 |
| 2024 Mayo | 0 | 23 | 23 |
| 2024 Abril | 0 | 25 | 25 |
| 2024 Marzo | 0 | 33 | 33 |
| 2024 Febrero | 0 | 29 | 29 |
| 2024 Enero | 0 | 32 | 32 |
| 2023 Diciembre | 0 | 38 | 38 |
| 2023 Noviembre | 0 | 46 | 46 |
| 2023 Octubre | 0 | 48 | 48 |
| 2023 Septiembre | 0 | 26 | 26 |
| 2023 Agosto | 0 | 26 | 26 |
| 2023 Julio | 0 | 66 | 66 |
| 2023 Junio | 0 | 43 | 43 |
| 2023 Mayo | 0 | 47 | 47 |
| 2023 Abril | 0 | 57 | 57 |
| 2023 Marzo | 3 | 38 | 41 |
| 2023 Febrero | 1 | 45 | 46 |
| 2023 Enero | 0 | 27 | 27 |
| 2022 Diciembre | 0 | 31 | 31 |
| 2022 Noviembre | 0 | 25 | 25 |
| 2022 Octubre | 0 | 25 | 25 |
| 2022 Septiembre | 0 | 35 | 35 |
| 2022 Agosto | 0 | 25 | 25 |
| 2022 Julio | 0 | 10 | 10 |
| 2022 Junio | 0 | 10 | 10 |
| 2022 Mayo | 0 | 16 | 16 |
| 2022 Abril | 0 | 10 | 10 |
| 2022 Marzo | 0 | 27 | 27 |
| 2022 Febrero | 0 | 12 | 12 |
| 2022 Enero | 0 | 29 | 29 |
| 2021 Diciembre | 0 | 14 | 14 |
| 2021 Noviembre | 0 | 16 | 16 |
| 2021 Octubre | 0 | 24 | 24 |
| 2021 Septiembre | 0 | 21 | 21 |
| 2021 Agosto | 0 | 25 | 25 |
| 2021 Julio | 0 | 15 | 15 |
| 2021 Junio | 0 | 8 | 8 |
| 2021 Mayo | 0 | 21 | 21 |
| 2021 Abril | 0 | 36 | 36 |
| 2021 Marzo | 0 | 22 | 22 |
| 2021 Febrero | 0 | 11 | 11 |
| 2021 Enero | 0 | 16 | 16 |
| 2020 Diciembre | 0 | 8 | 8 |
| 2020 Noviembre | 0 | 8 | 8 |
| 2020 Octubre | 0 | 5 | 5 |
| 2020 Septiembre | 1 | 8 | 9 |
| 2020 Agosto | 0 | 12 | 12 |
| 2020 Julio | 0 | 3 | 3 |
| 2020 Junio | 0 | 4 | 4 |
| 2020 Mayo | 0 | 7 | 7 |
| 2020 Abril | 0 | 1 | 1 |
| 2020 Marzo | 1 | 6 | 7 |
| 2020 Febrero | 0 | 2 | 2 |
| 2020 Enero | 0 | 6 | 6 |
| 2019 Diciembre | 0 | 13 | 13 |
| 2019 Noviembre | 1 | 7 | 8 |
| 2019 Octubre | 0 | 5 | 5 |
| 2019 Septiembre | 0 | 5 | 5 |
| 2019 Agosto | 0 | 2 | 2 |
| 2019 Julio | 0 | 6 | 6 |
| 2019 Junio | 0 | 4 | 4 |
| 2019 Mayo | 0 | 22 | 22 |
| 2017 Octubre | 3 | 1 | 4 |
| 2017 Septiembre | 8 | 8 | 16 |
| 2017 Agosto | 5 | 2 | 7 |
| 2017 Julio | 6 | 0 | 6 |
| 2017 Junio | 8 | 16 | 24 |
| 2017 Mayo | 11 | 3 | 14 |
| 2017 Abril | 7 | 1 | 8 |
| 2017 Marzo | 6 | 21 | 27 |
| 2017 Febrero | 23 | 5 | 28 |
| 2017 Enero | 11 | 2 | 13 |
| 2016 Diciembre | 20 | 3 | 23 |
| 2016 Noviembre | 27 | 2 | 29 |
| 2016 Octubre | 61 | 3 | 64 |
| 2016 Septiembre | 20 | 3 | 23 |
| 2016 Agosto | 14 | 1 | 15 |
| 2016 Julio | 15 | 2 | 17 |
| 2016 Junio | 26 | 0 | 26 |
| 2016 Mayo | 21 | 2 | 23 |
| 2016 Abril | 13 | 0 | 13 |
| 2016 Marzo | 19 | 2 | 21 |
| 2016 Febrero | 20 | 1 | 21 |
| 2016 Enero | 20 | 0 | 20 |
| 2015 Diciembre | 17 | 0 | 17 |
| 2015 Noviembre | 22 | 0 | 22 |
| 2015 Octubre | 22 | 0 | 22 |
| 2015 Septiembre | 28 | 0 | 28 |
| 2015 Agosto | 36 | 1 | 37 |
| 2015 Julio | 19 | 0 | 19 |
| 2015 Junio | 15 | 0 | 15 |
| 2015 Mayo | 14 | 0 | 14 |
| 2015 Abril | 12 | 0 | 12 |
| 2015 Marzo | 15 | 0 | 15 |
| 2015 Febrero | 16 | 0 | 16 |
| 2015 Enero | 28 | 0 | 28 |
| 2014 Diciembre | 9 | 0 | 9 |
| 2014 Noviembre | 4 | 0 | 4 |
| 2014 Octubre | 6 | 0 | 6 |
| 2014 Septiembre | 7 | 0 | 7 |
| 2014 Agosto | 14 | 0 | 14 |
| 2014 Julio | 11 | 0 | 11 |
| 2014 Junio | 17 | 0 | 17 |
| 2014 Mayo | 12 | 0 | 12 |
| 2014 Abril | 3 | 0 | 3 |
| 2014 Marzo | 3 | 0 | 3 |
| 2014 Febrero | 3 | 0 | 3 |
| 2014 Enero | 7 | 0 | 7 |
| 2013 Diciembre | 4 | 0 | 4 |
| 2013 Noviembre | 7 | 0 | 7 |
| 2013 Octubre | 11 | 0 | 11 |
| 2013 Septiembre | 4 | 0 | 4 |
| 2013 Agosto | 2 | 0 | 2 |
| 2000 Marzo | 1119 | 0 | 1119 |
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