metricas
covid
Buscar en
Annals of Hepatology
Toda la web
Inicio Annals of Hepatology Liquid biopsy of patients with advanced liver fibrosis reveals the association o...
Journal Information
Vol. 27. Issue S3.
Abstracts from XVII Mexican Congress of Hepatology
(December 2022)
Share
Share
Download PDF
More article options
Vol. 27. Issue S3.
Abstracts from XVII Mexican Congress of Hepatology
(December 2022)
Open Access
Liquid biopsy of patients with advanced liver fibrosis reveals the association of methylation in CpGs and miRNAs expression with the degree of severity
Visits
243
R De la Rosa-Bibiano1,2, R Escutia-Gutiérrez1, S Rodríguez-Sanabria1, E Cerda-Reyes2, JM Aguilar2, A Santos3, AS Sandoval-Rodríguez1, J Armendáriz-Borunda1,3,4
1 Institute of Molecular Biology in Medicine. CUCS. University of Guadalajara
2 Hospital Central Militar
3 Faculty of Medicine and Health Sciences. Tecnológico de Monterrey. Zapopan, Guadalajara, Mexico
4 Departments of Gastroenterology, Radiology, Pathology and Clinical Laboratory. Central Military Hospital. Mexico
This item has received

Under a Creative Commons license
Article information
Special issue
This article is part of special issue:
Vol. 27. Issue S3

Abstracts from XVII Mexican Congress of Hepatology

More info
Introduction and Objective

This study aimed to assess whether the expression of specific miRNAs and the percentage of DNA methylation of the Peroxisomal Proliferator-Activated Receptors (PPAR) α, γ and δ genes in tissue and liquid biopsy from patients with advanced liver fibrosis (F3 and F4) is associated to the degree of severity.

Materials and methods

Transjugular liver biopsy and liquid biopsy were collected from 23 patients with sustained viral response to the hepatitis C virus, with advanced residual fibrosis (F3 and F4). The percentage of methylation in CpG islands of the promoters of the PPARα, PPARγ and PPARδ genes and the expression levels of miRNAs were determined. Masson's trichrome hematoxylin-eosin staining was performed. DNA was extracted from tissues and plasma, and percent methylation was measured by pyrosequencing. Extraction and isolation of miRNAs from liver tissue were performed: miR-21, miR-34, miR-122, miR181b, miR192, miR-200a/b and their expression level compared to miR-16 was evaluated. The trial was approved by the research ethics committee, and informed consent was obtained.

Results

Higher promoter methylation percentages were observed in patients with more severe degrees of fibrosis (F4), both in tissue and in liquid biopsy. In addition, overexpression of miRNAs was associated with the degree of fibrosis.

Discussion

Epigenetic mechanisms (DNA methylation and microRNA expression) regulate the expression of multiple genes and their status may be a biomarker associated with the degree of fibrosis.

Conclusion

Liquid biopsy is an effective and accessible method for evaluating the degree of fibrosis.

Funding

The resources used in this study were from the hospital without any additional financing

Declaration of interest

The authors declare no potential conflicts of interest.

Full text is only aviable in PDF
Download PDF
Article options
es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos

Quizás le interese:
10.1016/j.aohep.2022.100863
No mostrar más