metricas
covid
Buscar en
Annals of Hepatology
Toda la web
Inicio Annals of Hepatology Genotype f prevails in Venezuelan urban patients with chronic hepatitis B(a)
Journal Information

Statistics

Follow this link to access the full text of the article

Genotype f prevails in Venezuelan urban patients with chronic hepatitis B(a)
Irma V. Machado
,
Corresponding author
intediag@movistar.net.ve
intediag@cantv.net

Correspondence and reprint request:
, María del Pilar Fortes*, Berta Vargas-Lovelle*, Aleidy C. Trómpiz*, Danerys A. López*, Roberto V. León, Merita Senior, Lucy Dagher§, Carmen E. López||, Elena Pestaña, Román Bacalao**, Miguel E. Garassini§
* Laboratorio Clínico Especializado Intediag-HV (Integración Diagnóstica en Hepatitis Viral), Caracas, Venezuela
Hospital General “Domingo Luciani” and Instituto Médico La Floresta, Caracas
Hospital de Clínicas Caracas
§ Centro Médico Docente La Trinidad, Caracas
|| Hospital de Niños “J.M. de Los Ríos”, Caracas
Fundación Zuliana del Hígado, Maracaibo, Estado Zulia and Policlínica Metropolitana, Caracas
** Centro Médico de Caracas
Read
1279
Times
was read the article
309
Total PDF
970
Total HTML
Share statistics
 array:24 [
  "pii" => "S1665268119316576"
  "issn" => "16652681"
  "doi" => "10.1016/S1665-2681(19)31657-6"
  "estado" => "S300"
  "fechaPublicacion" => "2010-04-01"
  "aid" => "71343"
  "copyright" => "Fundación Clínica Médica Sur, A.C."
  "copyrightAnyo" => "2010"
  "documento" => "article"
  "crossmark" => 0
  "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
  "subdocumento" => "fla"
  "cita" => "Ann Hepatol. 2010;9:172-6"
  "abierto" => array:3 [
    "ES" => true
    "ES2" => true
    "LATM" => true
  ]
  "gratuito" => true
  "lecturas" => array:2 [
    "total" => 50
    "formatos" => array:3 [
      "EPUB" => 9
      "HTML" => 20
      "PDF" => 21
    ]
  ]
  "itemSiguiente" => array:19 [
    "pii" => "S1665268119316588"
    "issn" => "16652681"
    "doi" => "10.1016/S1665-2681(19)31658-8"
    "estado" => "S300"
    "fechaPublicacion" => "2010-04-01"
    "aid" => "71344"
    "copyright" => "Fundación Clínica Médica Sur, A.C."
    "documento" => "article"
    "crossmark" => 0
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "fla"
    "cita" => "Ann Hepatol. 2010;9:177-80"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 52
      "formatos" => array:3 [
        "EPUB" => 7
        "HTML" => 22
        "PDF" => 23
      ]
    ]
    "en" => array:11 [
      "idiomaDefecto" => true
      "titulo" => "Serum concentrations of substance P in cholestasis"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => "en"
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "177"
          "paginaFinal" => "180"
        ]
      ]
      "contieneResumen" => array:1 [
        "en" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "resumenGrafico" => array:2 [
        "original" => 0
        "multimedia" => array:7 [
          "identificador" => "f0005"
          "etiqueta" => "Figure 1"
          "tipo" => "MULTIMEDIAFIGURA"
          "mostrarFloat" => true
          "mostrarDisplay" => false
          "figura" => array:1 [
            0 => array:4 [
              "imagen" => "gr1.jpeg"
              "Alto" => 606
              "Ancho" => 1002
              "Tamanyo" => 28123
            ]
          ]
          "descripcion" => array:1 [
            "en" => "<p id="sp0005" class="elsevierStyleSimplePara elsevierViewall">Mean &#177; SE serum concentrations of substance P in the study groups&#46; The mean serum concentration of substance P in the group of patients with chronic liver disease &#40;CLD&#41; and pruritus &#40;n &#61; 13&#41; was significantly higher than that of patients with chronic liver disease without pruritus &#40;n &#61; 17&#41;&#44; &#40;p &#60; 0&#46;0001&#41;&#44; and that of the control group of subjects &#40;n &#61; 12&#41; &#40;p &#60; 0&#46;0001&#41;&#46; The mean serum concentration of substance P from the group of patients with cholestasis without pruritus was not significantly different from that of the control group of subjects&#46;</p>"
          ]
        ]
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Mayur Trivedi, Nora V&#46; Bergasa"
          "autores" => array:2 [
            0 => array:2 [
              "nombre" => "Mayur"
              "apellidos" => "Trivedi"
            ]
            1 => array:3 [
              "preGrado" => "M&#46;D&#46;"
              "nombre" => "Nora"
              "apellidos" => "V&#46; Bergasa"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1665268119316588?idApp=UINPBA00004N"
    "url" => "/16652681/0000000900000002/v1_201907070740/S1665268119316588/v1_201907070740/en/main.assets"
  ]
  "itemAnterior" => array:19 [
    "pii" => "S1665268119316564"
    "issn" => "16652681"
    "doi" => "10.1016/S1665-2681(19)31656-4"
    "estado" => "S300"
    "fechaPublicacion" => "2010-04-01"
    "aid" => "71342"
    "copyright" => "Fundaci&#243;n Cl&#237;nica M&#233;dica Sur&#44; A&#46;C&#46;"
    "documento" => "article"
    "crossmark" => 0
    "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/"
    "subdocumento" => "fla"
    "cita" => "Ann Hepatol. 2010;9:166-71"
    "abierto" => array:3 [
      "ES" => true
      "ES2" => true
      "LATM" => true
    ]
    "gratuito" => true
    "lecturas" => array:2 [
      "total" => 72
      "formatos" => array:3 [
        "EPUB" => 11
        "HTML" => 18
        "PDF" => 43
      ]
    ]
    "en" => array:11 [
      "idiomaDefecto" => true
      "titulo" => "Efficacy of maintenance subcutaneous hepatitis B immune globulin &#40;HBIG&#41; post-transplant for prophylaxis against hepatitis B recurrence<span class="elsevierStyleSup">&#8224;</span>"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "tieneResumen" => "en"
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "166"
          "paginaFinal" => "171"
        ]
      ]
      "contieneResumen" => array:1 [
        "en" => true
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "resumenGrafico" => array:2 [
        "original" => 0
        "multimedia" => array:7 [
          "identificador" => "f0005"
          "etiqueta" => "Figure 1"
          "tipo" => "MULTIMEDIAFIGURA"
          "mostrarFloat" => true
          "mostrarDisplay" => false
          "figura" => array:1 [
            0 => array:4 [
              "imagen" => "gr1.jpeg"
              "Alto" => 661
              "Ancho" => 1003
              "Tamanyo" => 58716
            ]
          ]
          "descripcion" => array:1 [
            "en" => "<p id="sp0005" class="elsevierStyleSimplePara elsevierViewall">Mean anti-HBs titres post first injection over time&#46; The number of patients at each time value changes based on patient compliance with bloodwork each week&#44; and with those that need to be removed from this figure as they required a repeat injection&#44; such that n values are shown at each week&#46;</p>"
          ]
        ]
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Janakie Singham, Erica D&#46; Greanya, Kirby Lau, Siegfried R&#46; Erb, Nilu Partovi, Eric M&#46; Yoshida"
          "autores" => array:6 [
            0 => array:2 [
              "nombre" => "Janakie"
              "apellidos" => "Singham"
            ]
            1 => array:2 [
              "nombre" => "Erica D&#46;"
              "apellidos" => "Greanya"
            ]
            2 => array:2 [
              "nombre" => "Kirby"
              "apellidos" => "Lau"
            ]
            3 => array:2 [
              "nombre" => "Siegfried R&#46;"
              "apellidos" => "Erb"
            ]
            4 => array:2 [
              "nombre" => "Nilu"
              "apellidos" => "Partovi"
            ]
            5 => array:2 [
              "nombre" => "Eric M&#46;"
              "apellidos" => "Yoshida"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1665268119316564?idApp=UINPBA00004N"
    "url" => "/16652681/0000000900000002/v1_201907070740/S1665268119316564/v1_201907070740/en/main.assets"
  ]
  "en" => array:16 [
    "idiomaDefecto" => true
    "titulo" => "Genotype f prevails in Venezuelan urban patients with chronic hepatitis B<a class="elsevierStyleCrossRef" href="#fn1"><span class="elsevierStyleSup">&#40;a&#41;</span></a>"
    "tieneTextoCompleto" => true
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "172"
        "paginaFinal" => "176"
      ]
    ]
    "autores" => array:1 [
      0 => array:4 [
        "autoresLista" => "Irma V&#46; Machado, Mar&#237;a del Pilar Fortes, Berta Vargas-Lovelle, Aleidy C&#46; Tr&#243;mpiz, Danerys A&#46; L&#243;pez, Roberto V&#46; Le&#243;n, Merita Senior, Lucy Dagher, Carmen E&#46; L&#243;pez, Elena Pesta&#241;a, Rom&#225;n Bacalao, Miguel E&#46; Garassini"
        "autores" => array:12 [
          0 => array:4 [
            "nombre" => "Irma"
            "apellidos" => "V&#46; Machado"
            "email" => array:2 [
              0 => "intediag@movistar.net.ve"
              1 => "intediag@cantv.net"
            ]
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;</span>"
                "identificador" => "aff1"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;</span>"
                "identificador" => "cor1"
              ]
            ]
          ]
          1 => array:3 [
            "nombre" => "Mar&#237;a"
            "apellidos" => "del Pilar Fortes"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;</span>"
                "identificador" => "aff1"
              ]
            ]
          ]
          2 => array:3 [
            "nombre" => "Berta"
            "apellidos" => "Vargas-Lovelle"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;</span>"
                "identificador" => "aff1"
              ]
            ]
          ]
          3 => array:3 [
            "nombre" => "Aleidy C&#46;"
            "apellidos" => "Tr&#243;mpiz"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;</span>"
                "identificador" => "aff1"
              ]
            ]
          ]
          4 => array:3 [
            "nombre" => "Danerys A&#46;"
            "apellidos" => "L&#243;pez"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;</span>"
                "identificador" => "aff1"
              ]
            ]
          ]
          5 => array:3 [
            "nombre" => "Roberto V&#46;"
            "apellidos" => "Le&#243;n"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#8224;</span>"
                "identificador" => "aff2"
              ]
            ]
          ]
          6 => array:3 [
            "nombre" => "Merita"
            "apellidos" => "Senior"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#8225;</span>"
                "identificador" => "aff3"
              ]
            ]
          ]
          7 => array:3 [
            "nombre" => "Lucy"
            "apellidos" => "Dagher"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#167;</span>"
                "identificador" => "aff4"
              ]
            ]
          ]
          8 => array:3 [
            "nombre" => "Carmen E&#46;"
            "apellidos" => "L&#243;pez"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#124;&#124;</span>"
                "identificador" => "aff5"
              ]
            ]
          ]
          9 => array:3 [
            "nombre" => "Elena"
            "apellidos" => "Pesta&#241;a"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#182;</span>"
                "identificador" => "aff6"
              ]
            ]
          ]
          10 => array:3 [
            "nombre" => "Rom&#225;n"
            "apellidos" => "Bacalao"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#42;&#42;</span>"
                "identificador" => "aff7"
              ]
            ]
          ]
          11 => array:3 [
            "nombre" => "Miguel E&#46;"
            "apellidos" => "Garassini"
            "referencia" => array:1 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">&#167;</span>"
                "identificador" => "aff4"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:7 [
          0 => array:3 [
            "entidad" => "Laboratorio Cl&#237;nico Especializado Intediag-HV &#40;<span class="elsevierStyleItalic">Integraci&#243;n Diagn&#243;stica en Hepatitis Viral</span>&#41;&#44; Caracas&#44; Venezuela"
            "etiqueta" => "&#42;"
            "identificador" => "aff1"
          ]
          1 => array:3 [
            "entidad" => "Hospital General &#8220;Domingo Luciani&#8221; and Instituto M&#233;dico La Floresta&#44; Caracas"
            "etiqueta" => "<span class="elsevierStyleSup">&#8224;</span>"
            "identificador" => "aff2"
          ]
          2 => array:3 [
            "entidad" => "Hospital de Cl&#237;nicas Caracas"
            "etiqueta" => "<span class="elsevierStyleSup">&#8225;</span>"
            "identificador" => "aff3"
          ]
          3 => array:3 [
            "entidad" => "Centro M&#233;dico Docente La Trinidad&#44; Caracas"
            "etiqueta" => "<span class="elsevierStyleSup">&#167;</span>"
            "identificador" => "aff4"
          ]
          4 => array:3 [
            "entidad" => "Hospital de Ni&#241;os &#8220;J&#46;M&#46; de Los R&#237;os&#8221;&#44; Caracas"
            "etiqueta" => "<span class="elsevierStyleSup">&#124;&#124;</span>"
            "identificador" => "aff5"
          ]
          5 => array:3 [
            "entidad" => "Fundaci&#243;n Zuliana del H&#237;gado&#44; Maracaibo&#44; Estado Zulia and Policl&#237;nica Metropolitana&#44; Caracas"
            "etiqueta" => "<span class="elsevierStyleSup">&#182;</span>"
            "identificador" => "aff6"
          ]
          6 => array:3 [
            "entidad" => "Centro M&#233;dico de Caracas"
            "etiqueta" => "&#42;&#42;"
            "identificador" => "aff7"
          ]
        ]
        "correspondencia" => array:1 [
          0 => array:3 [
            "identificador" => "cor1"
            "etiqueta" => "&#42;"
            "correspondencia" => "Correspondence and reprint request&#58;"
          ]
        ]
      ]
    ]
    "resumenGrafico" => array:2 [
      "original" => 0
      "multimedia" => array:7 [
        "identificador" => "f0005"
        "etiqueta" => "Figure 1"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr1.jpeg"
            "Alto" => 711
            "Ancho" => 999
            "Tamanyo" => 49412
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="sp0005" class="elsevierStyleSimplePara elsevierViewall">HBV DNA viral loads shown by the group of urban patients with CHB&#44; genotype F&#44; e-antigen positive and e-antigen negative&#46; HBV DNA levels expressed as log<span class="elsevierStyleInf">10</span> IU&#47;mL&#44; were significantly higher &#40;P &#61; 0&#46;0001&#41; in 74 patients with CHB e-antigen positive compared to 16 patients with CHB e-antigen negative&#46;</p>"
        ]
      ]
    ]
    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Introduction</span><p id="p0005" class="elsevierStylePara elsevierViewall">Hepatitis B virus &#40;HBV&#41; has been classified into 8 genotypes &#40;A-H&#41; by means of molecular evolutionary genetics analysis&#46;<a class="elsevierStyleCrossRef" href="#bib0005"><span class="elsevierStyleSup">1</span></a> There is growing evidence that HBV genotypes may influence the natural history of liver disease including mode of transmission&#44; disease progression&#44; seroconversion to HBe antibody&#44; presence of HBV mutants and response to therapy&#46;<a class="elsevierStyleCrossRef" href="#bib0010"><span class="elsevierStyleSup">2</span></a> Today&#44; the application of nucleic acid tests for clinical management of chronic hepatitis B &#40;CHB&#41; is almost mandatory&#46;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> This battery of tests includes measurement of viral load and genotyping of emergent resistant viral mutants during therapy&#46;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a> Moreover&#44; in countries with high prevalence of HBsAg chronic carriers&#44; HBV genotyping has been introduced as valuable test in the clinical management of CHB&#46;<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">4</span></a></p><p id="p0010" class="elsevierStylePara elsevierViewall">Epidemiological data suggest that 7 to 12 million Latin Americans are infected with hepatitis B virus&#46;<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a> Currently in Venezuela&#44; the mean morbidity rate of HBV is approximately 3&#46;8 x 100&#46;000 inhabitants&#46; Moreover&#44; Venezuela is classified as having an intermediate prevalence of hepatitis B surface antigen &#40;HBsAg&#41; in urban areas&#46;<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">6</span></a><span class="elsevierStyleSup">&#44;</span><a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a> Venezuelan aborigine population is categorized as having the highest rate of HBsAg&#44;<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">6</span></a><span class="elsevierStyleSup">&#44;</span><a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a> and HBV genotype F has been well-documented as the most prevalent&#46;<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a> However&#44; there is scarce data regarding HBV genotypes in HBsAg chronic carriers from Venezuelan urban populations&#46; The present study was designed to identify the prevalence of HBV genotypes in native HBsAg chronic carriers in Venezuelan urban areas&#46; In addition&#44; we correlated the predominant HBV genotype with epidemiological&#44; serological and virological features of the HBV infection&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Subjects and Methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Patient population</span><p id="p0015" class="elsevierStylePara elsevierViewall">This study was approved by the Institutional Research Ethics Committee&#44; and written informed consent was obtained from each participant&#46; Patients with chronic HBV infection from different urban Venezuelan cities and referred to our tertiary diagnostic center &#40;Intediag-HV&#44; Caracas&#41; were recruited into this study&#46; Patients were not on treatment at the time of the investigation&#46; Non-Venezuelan migrant patients were excluded from the study&#46; Serum samples from each patient were collected&#44; aliquoted and stored at-20 &#176;C until analysis&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Serological and virological tests</span><p id="p0020" class="elsevierStylePara elsevierViewall">Hepatitis B e antigen &#40;HBeAg&#41; and antibodies &#40;anti-HBe&#41; were determined using the Axsym&#8482; immunoassay system &#40;Abbott Laboratories&#59; Chicago&#44; IL&#44; USA&#41;&#46; HBV DNA serum levels were measured by real-time PCR &#40;Real-Art&#174; HBV PCR assay&#44; &#40;Artus-Biotech&#44; Qiagen&#44; Hamburg&#44; Germany&#41;<a class="elsevierStyleCrossRefs" href="#bib0040"><span class="elsevierStyleSup">8</span></a><span class="elsevierStyleSup">-</span><a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">10</span></a> HBV genotypes were identified by PCR using type-specific primers in accordance to a commercial assay &#40;Human Hepatitis B Virus Genotyping&#44; Genekam Biote-chnology AG&#44; Duisburg&#44; Germany&#41;&#46;<a class="elsevierStyleCrossRef" href="#bib0055"><span class="elsevierStyleSup">11</span></a> Briefly&#44; a nested PCR is carried-out based on differences in the conserved nucleotides in the envelope ORFs followed by a second PCR using two mixes of primers &#40;group 1&#58; A 68 bp&#44; B 281 bp&#44; C 122 bp&#59; group 2&#58; D 119 bp&#44; E 167 bp&#44; F 97 bp&#41; to differentiate the HBV genotypes based on amplicon length&#46;</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Statistical analysis</span><p id="p0025" class="elsevierStylePara elsevierViewall">Viral loads data were presented as mean &#177; standard deviation &#40;SD&#41;&#46; In all cases&#44; the Kolmogorov-</p><p id="p0030" class="elsevierStylePara elsevierViewall">Smirnov test was applied to test for normal distribution &#40;Graphpad Instant software for Window version 305&#41;&#46; Unpaired Student <span class="elsevierStyleItalic">t</span> test was employed to compare differences in means of viral load levels between the e-antigen positive and the e-antigen negative HBsAg chronic carriers&#46; The significance level was assessed at <span class="elsevierStyleItalic">p</span> &#60; 0&#46;05&#46;</p></span></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Results</span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Patient population</span><p id="p0035" class="elsevierStylePara elsevierViewall">Our study population comprised of 90 patients with chronic HBV infection from different Venezuelan urban cities&#46; Patients were classified in two groups&#46; Group A consisted of 21 children and adolescents &#40;mean age 10 &#177; 6 years&#44; 11 males and 10 females&#41;&#46; The majority of patients were in the immune-tolerant phase of the infection and showing normal values of alanine-aminotransferase &#40;ALT&#41;&#46; Only two patients have mild elevation of ALT&#46; Patients acquired HBV either via blood transfusion or health-care measures during chemotherapy&#46; Group B comprised of 69 adults &#40;mean age 46 &#177; 12 years&#44; 36 males and 33 females&#41;&#44; and they presented elevations &#40;&#62; 2&#46;5 fold normal value&#41; or fluctuations of ALT values&#46; They mainly acquired HBV by sexual contact&#46;</p></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">HBeAg and anti-HBe</span><p id="p0040" class="elsevierStylePara elsevierViewall">All patients from group A were e-antigen positive&#46; In group B&#44; 53 patients showed e-antigen positive and 16 patients presented e-antigen negative with positive anti-HBe antibodies&#46; Overall&#44; 74 patients &#40;82&#46;2&#37;&#41; showed CHB e-antigen positive and 16 patients &#40;17&#46;8&#37;&#41; CHB e-antigen negative&#46;</p></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0055">HBV DNA circulating viral load and HBV genotypes</span><p id="p0045" class="elsevierStylePara elsevierViewall">The whole group showed HBV DNA levels ranging from 4&#46;1 to 8&#46;8 log<span class="elsevierStyleInf">10</span> IU&#47;mL&#46; No difference in viral load was found between groups A and B patient e-antigen positive&#46; However&#44; HBV DNA levels were significantly higher &#40;P &#61; 0&#46;0001&#41; in CHB patients with e-antigen positive compared with those with CHB e-antigen negative &#40;see <a class="elsevierStyleCrossRef" href="#f0005">Figure 1</a>&#41;&#46; Genotype F hepatitis B predominated in 88 patients &#40;97&#46;8&#37;&#41;&#46; Only two non-related patients &#40;child and adult&#41;&#44; both with CHB e-antigen positive&#44; showed to be co-infected with genotype A and genotype F hepatitis B &#40;<a class="elsevierStyleCrossRef" href="#f0010">Figure 2</a>&#41;&#46;</p><elsevierMultimedia ident="f0005"></elsevierMultimedia><elsevierMultimedia ident="f0010"></elsevierMultimedia></span></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0060">Discussion</span><p id="p0050" class="elsevierStylePara elsevierViewall">We have demonstrated that HBV genotype F prevails in Venezuelan urban populations infected with hepatitis B virus regardless of the immune status of patients&#46; This genotype was identified in either CHB e-antigen positive or e-antigen negative cases&#46; Moreover&#44; HBV genotype F was observed in patients who acquired the infection by different modes of transmission &#40;sexual&#44; blood transfusion&#44; chemotherapy&#41;&#46; Only two patients&#44; both in the active phase of the disease&#44; were co-infected with genotypes A and F&#46;</p><p id="p0055" class="elsevierStylePara elsevierViewall">HBV genotype F prevails in Amerindians from Western and Southern Venezuela&#46;<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a> In addition&#44; the circulation of this genotype has been reported in other communities such as hemodialysis patients and few patients with chronic hepatitis B&#46;<a class="elsevierStyleCrossRef" href="#bib0060"><span class="elsevierStyleSup">12</span></a> Our results are in good agreement with previously repor-ted data in South America&#46; Indeed&#44; genotype F has been predominantly found in groups of HBV patients from Colombia &#40;Venezuela&#8217;s eastern-border country&#41;&#44;<a class="elsevierStyleCrossRef" href="#bib0065"><span class="elsevierStyleSup">13</span></a> but differs from those observed in Brazil &#40;Venezuela&#8217;s southern border country&#41; where the frequency of genotype F is lower even in northern areas of this country&#46;<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">14</span></a> This disparity could be related to differences in the ethnic origins of Brazilian inhabitants&#46; Unlike the Venezuelan hybrid mestizo population &#40;Mongoloid&#44; Negroid and Caucasian&#41;&#44; the Native Amerindian inhabitants have only made a minor contribution to the Brazilian population as a whole&#44;<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">14</span></a><span class="elsevierStyleSup">&#44;</span><a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">15</span></a> We have now envisaged future genoty-ping studies including non-Venezuelan migrant HBV patients to determine whether this population exhibits a different genotype pattern&#46; Interestingly&#44; we thus far have identified two patients with genotype F and one with genotype F C C among five HBV carrier immigrants from China&#46; This suggests a high grade of circulation and transmission of genotype F among our urban populations&#46;</p><p id="p0060" class="elsevierStylePara elsevierViewall">Based on previous reports&#44; HBV genotype A has been associated to CHB e-antigen positive while genotypes B&#44; C and D &#40;often present precore mutants&#41; are serologically associated with positive anti-HBe antibodies&#46;<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a><span class="elsevierStyleSup">&#44;</span><a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">4</span></a> One of few published articles on HBV infection and genotype F documented that this pa-tient population&#44; who were all HBeAg positive&#44; had the lowest rate of remission&#46;<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">16</span></a> Furthermore&#44; the proportion of patients who presented with hepatic decompensation or died were higher than those infected with genotype A or D&#46; In addition&#44; none of these subjects cleared HBsAg from the serum&#46; We have previously reported that over 60&#37; of our adults HBV carriers have the presence of anti-HBe antibodies but they are clinically in inactive state&#46;<a class="elsevierStyleCrossRefs" href="#bib0085"><span class="elsevierStyleSup">17</span></a><span class="elsevierStyleSup">-</span><a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">20</span></a> In addition&#44; more than 80&#37; of patients with active disease presented CHB e-antigen positive and 12&#37; to 18&#37; CHB e-antigen negative&#46;<a class="elsevierStyleCrossRef" href="#bib0095"><span class="elsevierStyleSup">19</span></a><span class="elsevierStyleSup">&#44;</span><a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">20</span></a> In the present study&#44; we also found that 82&#37; of patients had CHB e-antigen positive and significantly higher viral load&#46; Taken together&#44; although these results demonstrate that genotype F is mainly associated with CHB e-an-tigen positive&#44; we have to be aware that an important segment of our HBsAg carriers with positive anti-HBe antibodies might represent CHB e-antigen negative instead of inactive HBV carrier state&#46; Therefore&#44; we have to make the effort to quantify HBV-DNA levels to assess the appropriate diagnosis and management of these patients&#46;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a><span class="elsevierStyleSup">&#44;</span><a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">22</span></a></p><p id="p0065" class="elsevierStylePara elsevierViewall">A recently published study among Alaska Native young populations infected with HBV demonstrated that genotype F strain compared to genotypes A&#44; B&#44; C or D was significantly associated with the occurrence of hepatocellular carcinoma &#40;HCC&#41;&#46;<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">23</span></a> The incidence of HBV-induced HCC has not been accurately established in our country&#46; However&#44; Leon&#44; <span class="elsevierStyleItalic">et &#945;l&#46;</span> reported that over a 8-year period they had diagnosed 14 patients infected with HBV &#40;40-50 years old&#41; who developed HCC&#44; twelve of them already with liver cirrhosis and two without&#46;<a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">20</span></a> Another study compiling 68 Venezuelan patients with HCC&#44; most of them in the sixth decade of life&#44; described HBV as the main etiologic agent in 347 of cases&#46;<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">24</span></a> The development of cirrhosis and liver cancer during the fourth and sixth decade of life has also been described in other Latin American countries&#44; where HBV transmission occurs primarily from sexual contact like in Venezuelan urban areas&#46;<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">25</span></a><span class="elsevierStyleSup">&#44;</span><a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">26</span></a> On the other hand&#44; the progress to chronic liver disease and HCC in Asian HBV carriers&#44; who have acquired the infection perinatally&#44; occurs early in childhood&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> In Venezuela&#44; these complications in HBV-infected children have been described in four &#40;10&#46;2 7&#41; out of thirty nine children over a 9-year follow-up&#46;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">28</span></a> However&#44; they acquired the infection during chemotherapy rather than perinatally&#46; This finding is not surprising since nosocomial HBV infection in Venezuela remains a common unsolved problem nationwide from many decades ago&#46;<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">29</span></a><span class="elsevierStyleSup">&#44;</span><a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">30</span></a> It is noteworthy to mention that thus far our children and adolescents with CHB do not seroconvert earlier to antiH-Be antibodies&#46;</p><p id="p0070" class="elsevierStylePara elsevierViewall">In conclusion&#44; we have demonstrated that genotype F is the main circulating HBV strain among HB-sAg carriers from Venezuelan urban areas&#46; This genotype is associated mostly with CHB e-antigen positive and showed a high rate of transmission&#46; Progression to cirrhosis and hepatocellular carcinoma could be major clinical events of this patient population independently of age at acquisition or transmission route&#46; Longitudinal studies are encouraged to further describe the natural history of this disease in Venezuela&#46;</p></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Abbreviations</span><p id="p0075" class="elsevierStylePara elsevierViewall"><ul class="elsevierStyleList" id="li0005"><li class="elsevierStyleListItem" id="list0005"><span class="elsevierStyleLabel">&#8226;</span><p id="p0080" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">HBV&#58;</span> Hepatitis B Virus</p></li><li class="elsevierStyleListItem" id="list0010"><span class="elsevierStyleLabel">&#8226;</span><p id="p0085" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">HBsAg&#58;</span> Hepatitis B surface Antigen</p></li><li class="elsevierStyleListItem" id="list0015"><span class="elsevierStyleLabel">&#8226;</span><p id="p0090" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">CHB&#58;</span> Chronic hepatitis B</p></li><li class="elsevierStyleListItem" id="list0020"><span class="elsevierStyleLabel">&#8226;</span><p id="p0095" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">HBeAg&#58;</span> Hepatitis B e antigen</p></li><li class="elsevierStyleListItem" id="list0025"><span class="elsevierStyleLabel">&#8226;</span><p id="p0100" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">anti-HBe&#58;</span> Hepatitis Be antibody</p></li><li class="elsevierStyleListItem" id="list0030"><span class="elsevierStyleLabel">&#8226;</span><p id="p0105" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">PCR&#58;</span> Polymerase Chain Reaction</p></li><li class="elsevierStyleListItem" id="list0035"><span class="elsevierStyleLabel">&#8226;</span><p id="p0110" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">SD&#58;</span> Standard deviation</p></li><li class="elsevierStyleListItem" id="list0040"><span class="elsevierStyleLabel">&#8226;</span><p id="p0115" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">HCC&#58;</span> Hepatocellular carcinoma</p></li></ul></p></span></span>"
    "textoCompletoSecciones" => array:1 [
      "secciones" => array:8 [
        0 => array:3 [
          "identificador" => "xres1217531"
          "titulo" => "Abstract"
          "secciones" => array:1 [
            0 => array:1 [
              "identificador" => "abs0005"
            ]
          ]
        ]
        1 => array:2 [
          "identificador" => "xpalclavsec1132912"
          "titulo" => "Key words"
        ]
        2 => array:2 [
          "identificador" => "sec0005"
          "titulo" => "Introduction"
        ]
        3 => array:3 [
          "identificador" => "sec0010"
          "titulo" => "Subjects and Methods"
          "secciones" => array:3 [
            0 => array:2 [
              "identificador" => "sec0015"
              "titulo" => "Patient population"
            ]
            1 => array:2 [
              "identificador" => "sec0020"
              "titulo" => "Serological and virological tests"
            ]
            2 => array:2 [
              "identificador" => "sec0025"
              "titulo" => "Statistical analysis"
            ]
          ]
        ]
        4 => array:3 [
          "identificador" => "sec0030"
          "titulo" => "Results"
          "secciones" => array:3 [
            0 => array:2 [
              "identificador" => "sec0035"
              "titulo" => "Patient population"
            ]
            1 => array:2 [
              "identificador" => "sec0040"
              "titulo" => "HBeAg and anti-HBe"
            ]
            2 => array:2 [
              "identificador" => "sec0045"
              "titulo" => "HBV DNA circulating viral load and HBV genotypes"
            ]
          ]
        ]
        5 => array:2 [
          "identificador" => "sec0050"
          "titulo" => "Discussion"
        ]
        6 => array:2 [
          "identificador" => "sec0055"
          "titulo" => "Abbreviations"
        ]
        7 => array:1 [
          "titulo" => "Reference"
        ]
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "PalabrasClave" => array:1 [
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "Key words"
          "identificador" => "xpalclavsec1132912"
          "palabras" => array:5 [
            0 => "Genotype F"
            1 => "Chronic hepatitis B"
            2 => "e-entigen"
            3 => "Anti-eHB antibodies"
            4 => "Urban HBV carrier"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "resumen" => array:1 [
      "en" => array:2 [
        "titulo" => "Abstract"
        "resumen" => "<span id="abs0005" class="elsevierStyleSection elsevierViewall"><p id="sp0015" class="elsevierStyleSimplePara elsevierViewall">Hepatitis B virus &#40;HBV&#41; has been classified into 8 genotypes &#40;A-H&#41;&#46; Genotypes A&#44; D and F have been identified in some South American countries&#44; but in Venezuela studies have been more restricted to aboriginal communities where genotype F is predominant&#46; The aim of the present study was to identify the prevalence of HBV genotypes among native HBsAg carriers in Venezuelan urban areas&#46; In addition&#44; we correlated the predominant HBV genotype with epidemiological&#44; serological and virological features of the infection&#46; Non-Venezuelan migrant patients were excluded from this study&#46; Serum samples from 90 patients &#40;21 children and 69 adults&#41; with chronic hepatitis B &#40;CHB&#41; were analyzed&#46; Seventy-four patients had CHB e-antigen positive and 16 CHB e-antigen negative&#46; HBV DNA serum levels of the whole group ranged from 4&#46;1 to 8&#46;8 log<span class="elsevierStyleInf">1H</span> IU&#47;mL&#46; Patients with CHB e-antigen positive showed significantly higher viral loads &#40;P &#61; 0&#46;0001&#41; than the group with CHB e-antigen negative&#46; Eighty-eight patients &#40;97&#46;8&#37;&#41; exhibited HBV genotype F while two non-related patients &#40;2&#46;2&#37;&#41; were infected with A &#43; F genotypes&#46; Genotype F is the main circulating HBV strain among HBsAg carriers from Venezuelan urban areas&#46; This genotype is associated mostly with CHB e-antigen positive and high rate of transmission&#46; Progression to cirrhosis and hepatocellular carcinoma could be major clinical events of this patient population independently of age at acquisition or transmission route&#46;</p></span>"
      ]
    ]
    "NotaPie" => array:1 [
      0 => array:3 [
        "etiqueta" => "<span class="elsevierStyleSup">&#40;a&#41;</span>"
        "nota" => "<p class="elsevierStyleNotepara" id="np0005">This project was supported&#44; in part&#44; by an award to Intediag-HV granted by Fondo Pro-Salud 2007&#44; C&#225;mara Venezolana de Fabricantes de Cerveza &#40;CAVEFACE&#41;&#44; Venezuela&#46; A preliminary report of this work was presented in an abstract form at the 13<span class="elsevierStyleSup">th</span> International Symposium on Viral Hepatitis and Liver Disease&#44; March&#44; 2009&#44; Washington&#44; USA&#46; www&#46;ishld2009&#46;org&#47;pdf&#47;ISVHLD&#95;Poster&#95;Presentation&#95;Abstractspdf</p>"
        "identificador" => "fn1"
      ]
    ]
    "multimedia" => array:2 [
      0 => array:7 [
        "identificador" => "f0005"
        "etiqueta" => "Figure 1"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr1.jpeg"
            "Alto" => 711
            "Ancho" => 999
            "Tamanyo" => 49412
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="sp0005" class="elsevierStyleSimplePara elsevierViewall">HBV DNA viral loads shown by the group of urban patients with CHB&#44; genotype F&#44; e-antigen positive and e-antigen negative&#46; HBV DNA levels expressed as log<span class="elsevierStyleInf">10</span> IU&#47;mL&#44; were significantly higher &#40;P &#61; 0&#46;0001&#41; in 74 patients with CHB e-antigen positive compared to 16 patients with CHB e-antigen negative&#46;</p>"
        ]
      ]
      1 => array:7 [
        "identificador" => "f0010"
        "etiqueta" => "Figure 2"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr2.jpeg"
            "Alto" => 720
            "Ancho" => 844
            "Tamanyo" => 34013
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="sp0010" class="elsevierStyleSimplePara elsevierViewall">HBV genotypes identified in urban patients with CHB&#46; Genotype F prevails in 97&#46;8&#37; of urban patients with CHB irrespective of e antigen status&#46; Two not-related patients &#40;2&#46;2&#37;&#41; were infected with genotype A and genotype F&#46;</p>"
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "Reference"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bs0005"
          "bibliografiaReferencia" => array:30 [
            0 => array:3 [
              "identificador" => "bib0005"
              "etiqueta" => "1&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Completed genomes&#44; phylogenetic relatedness&#44; and structural proteins of six strains of the hepatitis B virus&#44; four of which represent two new genotypes"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "Norder H&#46;"
                            1 => "Courouce A&#46;M&#46;"
                            2 => "Magnius L&#46;O&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1006/viro.1994.1060"
                      "Revista" => array:6 [
                        "tituloSerie" => "Virology"
                        "fecha" => "1994"
                        "volumen" => "198"
                        "paginaInicial" => "489"
                        "paginaFinal" => "503"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8291231"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0010"
              "etiqueta" => "2&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Clinical Significance of Hepatitis B Virus Genotypes&#44; Variants&#44; and Mutants"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "Wai C&#46;H&#46;-T"
                            1 => "Fontana R&#46;J&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Clin Liv Dis"
                        "fecha" => "2004"
                        "volumen" => "8"
                        "paginaInicial" => "321"
                        "paginaFinal" => "352"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0015"
              "etiqueta" => "3&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Genotyping and Genomic Sequencing in Clinical Practice"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "Gish R&#46;G&#46;"
                            1 => "Locarnini S&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Clin Liv Dis"
                        "fecha" => "2007"
                        "volumen" => "11"
                        "paginaInicial" => "761"
                        "paginaFinal" => "795"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib0020"
              "etiqueta" => "4&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Factors influencing liver disease progression in chronic hepatitis B"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "Liaw Y&#46;F&#46;"
                            1 => "Sollano J&#46;D&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/j.1478-3231.2005.01178.x"
                      "Revista" => array:7 [
                        "tituloSerie" => "Liver Int"
                        "fecha" => "2006"
                        "volumen" => "26"
                        "numero" => "Suppl&#46; 2"
                        "paginaInicial" => "23"
                        "paginaFinal" => "29"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16420506"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib0025"
              "etiqueta" => "5&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Hepatitis B and hepatitis Delta virus infection in South America"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "Torres J&#46;R&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1136/gut.38.suppl_2.s48"
                      "Revista" => array:7 [
                        "tituloSerie" => "Gut"
                        "fecha" => "1996"
                        "volumen" => "38"
                        "numero" => "Suppl&#46; 2"
                        "paginaInicial" => "S48"
                        "paginaFinal" => "S55"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8786054"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib0030"
              "etiqueta" => "6&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Seroepidemiological differences of HBV infection between urban areas and Amerindian population in Venezuela"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "Machado I&#46;V&#46;"
                            1 => "Carvajal J&#46;"
                            2 => "Mondolfi A&#46;"
                            3 => "Marcano N&#46;"
                            4 => "Yarzabal L&#46;"
                            5 => "Bianco N&#46;E&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "LibroEditado" => array:5 [
                        "editores" => "ZuckermanA&#46;J&#46;"
                        "titulo" => "Viral Hepatitis and Liver Disease"
                        "paginaInicial" => "174"
                        "paginaFinal" => "176"
                        "serieFecha" => "1988"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib0035"
              "etiqueta" => "7&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Hepatitis B virus genetic diversity in Latin America"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "Devesa M&#46;"
                            1 => "Pujol F&#46;H&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.virusres.2007.01.004"
                      "Revista" => array:6 [
                        "tituloSerie" => "Virus Res"
                        "fecha" => "2007"
                        "volumen" => "127"
                        "paginaInicial" => "177"
                        "paginaFinal" => "184"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17280734"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib0040"
              "etiqueta" => "8&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Virological techniques for the diagnosis and monitoring of hepatitis B and C"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "Chevaliez S&#46;"
                            1 => "Pawlotsky J&#46;-M&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Ann Hepatol"
                        "fecha" => "2009"
                        "volumen" => "8"
                        "paginaInicial" => "7"
                        "paginaFinal" => "12"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19221527"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib0045"
              "etiqueta" => "9&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Rapid Quantification of hepatitis B virus DNA by automated sample preparation and real-time PCR"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "Stelz E&#46;"
                            1 => "Muller Z&#46;"
                            2 => "Marth E&#46;"
                            3 => "Kessler H&#46;H&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1128/JCM.42.6.2445-2449.2004"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "2004"
                        "volumen" => "42"
                        "paginaInicial" => "2445"
                        "paginaFinal" => "2449"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15184417"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib0050"
              "etiqueta" => "10&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "La frecuencia del genotipo 1 del virus de la hepatitis C no ha variado en Venezuela"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "Fortes M&#46;P&#46;"
                            1 => "Tr&#243;mpiz A&#46;"
                            2 => "Can&#243;nico Y&#46;"
                            3 => "Vargas-Lovelle B&#46;"
                            4 => "Machado I&#46;V&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Rev Col Gastroenterol"
                        "fecha" => "2009"
                        "volumen" => "24"
                        "paginaInicial" => "256"
                        "paginaFinal" => "258"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib0055"
              "etiqueta" => "11&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Rapid and specific genotyping system for hepatitis B virus corresponding to six major genotypes by PCR using type-specific primers"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "Naito H&#46;"
                            1 => "Hayashi S&#46;"
                            2 => "Abe K&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1128/JCM.39.1.362-364.2001"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "2001"
                        "volumen" => "39"
                        "paginaInicial" => "362"
                        "paginaFinal" => "364"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11136801"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            11 => array:3 [
              "identificador" => "bib0060"
              "etiqueta" => "12&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Antigenic Diversity of Hepatitis B Virus Strains of Genotype F in Amerindians and Other Population Groups from Venezuela"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:7 [
                            0 => "Blitz L&#46;"
                            1 => "Pujol F&#46;H&#46;"
                            2 => "Swenson P&#46;D&#46;"
                            3 => "Porto L&#46;"
                            4 => "Atencio R&#46;"
                            5 => "Araujo M&#46;"
                            6 => "Costa L&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "1998"
                        "volumen" => "36"
                        "paginaInicial" => "648"
                        "paginaFinal" => "651"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9508289"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib0065"
              "etiqueta" => "13&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Subgenotype diversity of hepatitis B virus American genotype F in Amerindians from Venezuela and the general population of Colombia"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:7 [
                            0 => "Devesa M&#46;"
                            1 => "Loureiro C&#46;L&#46;"
                            2 => "Rivas Y&#46;"
                            3 => "Monsalve F&#46;"
                            4 => "Cardona N&#46;"
                            5 => "Duarte M&#46;C&#46;"
                            6 => "Poblete F&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1002/jmv.21024"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Med Virol"
                        "fecha" => "2008"
                        "volumen" => "80"
                        "paginaInicial" => "20"
                        "paginaFinal" => "26"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18041024"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib0070"
              "etiqueta" => "14&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Hepatitis B virus genotypes circulating in Brazil&#58; molecular characterization of genotypes F isolates"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:7 [
                            0 => "Mello F&#46;C&#46;A&#46;"
                            1 => "Souto F&#46;J&#46;D&#46;"
                            2 => "Tabuco L&#46;C&#46;"
                            3 => "Villela-Nogueira C&#46;A&#46;"
                            4 => "Coelho H&#46;S&#46;M&#46;"
                            5 => "Franz H&#46;C&#46;F&#46;"
                            6 => "Saraiva J&#46;C&#46;P&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1186/1471-2180-7-103"
                      "Revista" => array:5 [
                        "tituloSerie" => "BMC Microbiology"
                        "fecha" => "2007"
                        "volumen" => "7"
                        "paginaInicial" => "103"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18036224"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            14 => array:3 [
              "identificador" => "bib0075"
              "etiqueta" => "15&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "HLA class I and class II allele and haplotype distribution in the Venezuelan population"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "Makhatadze N&#46;"
                            1 => "Franco M&#46;T&#46;"
                            2 => "Layrisse Z&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Hum Immunol"
                        "fecha" => "1997"
                        "volumen" => "55"
                        "paginaInicial" => "53"
                        "paginaFinal" => "58"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9328790"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            15 => array:3 [
              "identificador" => "bib0080"
              "etiqueta" => "16&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Influence of hepatitis B virus genotype on the long-term outcome of chronic hepatitis B in Western patients"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => "S&#225;nchez-Tapias J&#46;M&#46;"
                            1 => "Costa J&#46;"
                            2 => "Mas A&#46;"
                            3 => "Bruguera M&#46;"
                            4 => "Rodes J&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1053/gast.2002.37041"
                      "Revista" => array:6 [
                        "tituloSerie" => "Gastroenterology"
                        "fecha" => "2002"
                        "volumen" => "123"
                        "paginaInicial" => "1848"
                        "paginaFinal" => "1856"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12454842"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            16 => array:3 [
              "identificador" => "bib0085"
              "etiqueta" => "17&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Assessment of former and newly developed HBV assays in a third world setting"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "Zabaleta M&#46;E&#46;"
                            1 => "Toro F&#46;I&#46;"
                            2 => "Ruiz M&#46;E&#46;"
                            3 => "Colmenares C&#46;J&#46;"
                            4 => "Bianco N&#46;E&#46;"
                            5 => "Machado I&#46;V&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "J Med Virol"
                        "fecha" => "1992"
                        "volumen" => "38"
                        "paginaInicial" => "240"
                        "paginaFinal" => "245"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/1474375"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            17 => array:3 [
              "identificador" => "bib0090"
              "etiqueta" => "18&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Abordaje Inmunocl&#237;nico&#44; Molecular e Inmunopatol&#243;gico de la hepatitis cr&#243;nica viral&#46; Consideraciones Terap&#233;uticas"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:7 [
                            0 => "Machado I&#46;V&#46;"
                            1 => "Deibis L&#46;"
                            2 => "Risquez E&#46;"
                            3 => "Tassinari P&#46;"
                            4 => "Zabaleta M&#46;E&#46;"
                            5 => "Toro F&#46;I&#46;"
                            6 => "Baroja M&#46;L&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "GEN"
                        "fecha" => "1994"
                        "volumen" => "48"
                        "paginaInicial" => "124"
                        "paginaFinal" => "132"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            18 => array:3 [
              "identificador" => "bib0095"
              "etiqueta" => "19&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "T-cell hyperreactivity to IL-6 in chronic non-viremic HBV carriers despite normal IL-6 receptor or gp130 expression"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:7 [
                            0 => "Baroja M&#46;L&#46;"
                            1 => "Sirit F&#46;L&#46;"
                            2 => "Toro F&#46;I&#46;"
                            3 => "Rivera H&#46;"
                            4 => "Garzaro J&#46;"
                            5 => "Bianco N&#46;E&#46;"
                            6 => "Machado I&#46;V&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1089/vim.1996.9.187"
                      "Revista" => array:6 [
                        "tituloSerie" => "Viral Immunol"
                        "fecha" => "1996"
                        "volumen" => "9"
                        "paginaInicial" => "187"
                        "paginaFinal" => "194"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8890477"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            19 => array:3 [
              "identificador" => "bib0100"
              "etiqueta" => "20&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "A further definition of the major consequences of chronic hepatitis B in Venezuela"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "Le&#243;n R&#46;V&#46;"
                            1 => "Machado I&#46;V&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "J Clin Virol"
                        "fecha" => "2006"
                        "volumen" => "36"
                        "numero" => "Suppl&#46; 2"
                        "paginaInicial" => "S208"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            20 => array:3 [
              "identificador" => "bib0105"
              "etiqueta" => "21&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "J Hepatol"
                      "autores" => array:1 [
                        0 => array:2 [
                          "colaboracion" => "EASL Clinical Practice Guidelines&#58; Management of chronic hepatitis B&#46;"
                          "etal" => false
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:4 [
                        "fecha" => "2009"
                        "volumen" => "50"
                        "paginaInicial" => "227"
                        "paginaFinal" => "242"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            21 => array:3 [
              "identificador" => "bib0110"
              "etiqueta" => "22&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Barriers to care of chronic hepatitis patients in Latin America"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "Strauss E&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.arcmed.2007.02.002"
                      "Revista" => array:6 [
                        "tituloSerie" => "Arch Med Res"
                        "fecha" => "2007"
                        "volumen" => "38"
                        "paginaInicial" => "711"
                        "paginaFinal" => "715"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17613362"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            22 => array:3 [
              "identificador" => "bib0115"
              "etiqueta" => "23&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Hepatitis B virus genotypes in Alaska Native people with hepatocellular carcinoma&#58; preponderance of genotype F"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:7 [
                            0 => "Livingston S&#46;E&#46;"
                            1 => "Simonetti J&#46;P&#46;"
                            2 => "McMahon B&#46;J&#46;"
                            3 => "Bulkow L&#46;R&#46;"
                            4 => "Hurlburt K&#46;J&#46;"
                            5 => "Homan C&#46;E&#46;"
                            6 => "Snowball M&#46;M&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1086/509894"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Infect Dis"
                        "fecha" => "2007"
                        "volumen" => "195"
                        "paginaInicial" => "5"
                        "paginaFinal" => "11"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17152003"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            23 => array:3 [
              "identificador" => "bib0120"
              "etiqueta" => "24&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "An&#225;lisis de caracter&#237;sticas etiol&#243;gicas y demogr&#225;ficas asociadas a carcinoma hepatocelular en pacientes tratados en un Servicio de Radiolog&#237;a Intervencionista de Referencia"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "D&#237;az M&#46;S&#46;"
                            1 => "Bastrado G&#46;N&#46;A&#46;"
                            2 => "Roizental M&#46;"
                            3 => "Dagher L&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:4 [
                        "tituloSerie" => "Ann Hepatol"
                        "fecha" => "2008"
                        "volumen" => "302"
                        "paginaInicial" => "8"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            24 => array:3 [
              "identificador" => "bib0125"
              "etiqueta" => "25&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Special aspects of Hepatitis B Virus and Delta Virus Infection in Latin America"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "Torres J&#46;R&#46;"
                            1 => "Machado I&#46;V&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Inf Dis Clin North Am"
                        "fecha" => "1994"
                        "volumen" => "8"
                        "paginaInicial" => "13"
                        "paginaFinal" => "27"
                        "itemHostRev" => array:3 [
                          "pii" => "S0302283812004861"
                          "estado" => "S300"
                          "issn" => "03022838"
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            25 => array:3 [
              "identificador" => "bib0130"
              "etiqueta" => "26&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Assessment of Delta Virus Infection in Venezuelan high-risk population for hepatitis B virus"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "Machado I&#46;V&#46;"
                            1 => "Deibis L&#46;"
                            2 => "Zabaleta M&#46;"
                            3 => "Toro F&#46;I&#46;"
                            4 => "Mari&#241;o V&#46;"
                            5 => "Ram&#237;rez J&#46;L&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Clin Biol Res"
                        "fecha" => "1991"
                        "volumen" => "364"
                        "paginaInicial" => "105"
                        "paginaFinal" => "113"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            26 => array:3 [
              "identificador" => "bib0135"
              "etiqueta" => "27&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Hepatocellular Carcinoma&#58; Epidemiology and Molecular Carcinogenesis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "EL-Serag H&#46;B&#46;"
                            1 => "Lenhard-Rudolph K&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1053/j.gastro.2007.04.061"
                      "Revista" => array:6 [
                        "tituloSerie" => "Gastroenterology"
                        "fecha" => "2007"
                        "volumen" => "132"
                        "paginaInicial" => "2557"
                        "paginaFinal" => "2576"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17570226"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            27 => array:3 [
              "identificador" => "bib0140"
              "etiqueta" => "28&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Hepatitis B y C en pacientes pedi&#225;tricos oncol&#243;gicos"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "L&#243;pez C&#46;E&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:4 [
                        "tituloSerie" => "Rev Chil Nut"
                        "fecha" => "2009"
                        "volumen" => "36"
                        "paginaInicial" => "85"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            28 => array:3 [
              "identificador" => "bib0145"
              "etiqueta" => "29&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Riesgo de exposici&#243;n ocupacional al virus de la hepatitis B en personal de salud venezolano&#46; Estudio multic&#233;ntrico"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:7 [
                            0 => "Machado I&#46;V&#46;"
                            1 => "Marcano N&#46;B&#46;"
                            2 => "Rosales A&#46;"
                            3 => "Rinc&#243;n R&#46;"
                            4 => "Carvajal J&#46;"
                            5 => "Rivero M&#46;T&#46;"
                            6 => "Mu&#241;oz J&#46;F&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "GEN"
                        "fecha" => "1990"
                        "volumen" => "44"
                        "paginaInicial" => "1"
                        "paginaFinal" => "8"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            29 => array:3 [
              "identificador" => "bib0150"
              "etiqueta" => "30&#46;"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Costo-beneficio de la vacunaci&#243;n contra la hepatitis B en trabajadores de hospitales de Venezuela"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "Fern&#225;ndez Barboza R&#46;"
                            1 => "Rivero D&#46;"
                            2 => "Echeverr&#237;a B&#46;"
                            3 => "Machado I&#46;V&#46;"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Bol Of Sanit Panam"
                        "fecha" => "1991"
                        "volumen" => "111"
                        "paginaInicial" => "16"
                        "paginaFinal" => "23"
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
  ]
  "idiomaDefecto" => "en"
  "url" => "/16652681/0000000900000002/v1_201907070740/S1665268119316576/v1_201907070740/en/main.assets"
  "Apartado" => array:4 [
    "identificador" => "77721"
    "tipo" => "SECCION"
    "en" => array:2 [
      "titulo" => "Original Article"
      "idiomaDefecto" => true
    ]
    "idiomaDefecto" => "en"
  ]
  "PDF" => "https://static.elsevier.es/multimedia/16652681/0000000900000002/v1_201907070740/S1665268119316576/v1_201907070740/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1665268119316576?idApp=UINPBA00004N"
]
Article information
ISSN: 16652681
Original language: English
The statistics are updated each day
Year/Month Html Pdf Total
2024 November 2 0 2
2024 October 13 1 14
2024 September 24 2 26
2024 August 17 1 18
2024 July 10 3 13
2024 June 13 0 13
2024 May 13 2 15
2024 April 14 2 16
2024 March 21 7 28
2024 February 21 5 26
2024 January 23 1 24
2023 December 9 5 14
2023 November 24 5 29
2023 October 13 2 15
2023 September 23 4 27
2023 August 13 3 16
2023 July 5 2 7
2023 June 16 2 18
2023 May 52 3 55
2023 April 57 1 58
2023 March 43 2 45
2023 February 21 0 21
2023 January 40 10 50
2022 December 33 4 37
2022 November 40 4 44
2022 October 28 7 35
2022 September 8 12 20
2022 August 14 5 19
2022 July 17 9 26
2022 June 10 7 17
2022 May 9 9 18
2022 April 12 10 22
2022 March 9 9 18
2022 February 9 5 14
2022 January 24 7 31
2021 December 45 10 55
2021 November 22 7 29
2021 October 18 9 27
2021 September 24 14 38
2021 August 7 4 11
2021 July 10 7 17
2021 June 7 10 17
2021 May 10 4 14
2021 April 23 13 36
2021 March 7 8 15
2021 February 8 5 13
2021 January 5 14 19
2020 December 6 4 10
2020 November 6 8 14
2020 October 3 5 8
2020 September 10 6 16
2020 August 14 1 15
2020 July 4 2 6
2020 June 3 1 4
2020 May 6 3 9
2020 April 3 0 3
2020 March 8 1 9
2020 February 4 2 6
2020 January 1 3 4
2019 December 4 7 11
2019 November 4 0 4
2019 October 1 2 3
2019 September 4 0 4
2019 August 1 0 1
2019 July 2 8 10
Show all

Follow this link to access the full text of the article

es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos