P-8 EXTRACELLULAR VESICLE-DERIVED MICRORNA SIGNATURE IN HCV AND HCV/HIV PATIENTS WITH DIFFERENT STAGES OF LIVER FIBROSIS
Victoria Cairoli1, Daniel Valle Millares2, Pablo Ryan3, Lourdes Dominguez4, Luz Martín-Carbonero5, Beatriz Ameigeiras6, Verónica Briz2, Amanda Fernandez Rodriguez2,7, Ma Victoria Preciado1, Pamela Valva1
1 Multidisciplinary Institute for Pediatric Pathology Research (Imipp-Conicet-Gcba), Buenos Aires, Argentina
2 National Microbiology Center, Instituto de Salud Carlos III, Madrid, España
3 Infectious Diseases Department, Hospital Universitario Infanta Leonor, Madrid, España
4 HIV Unit. Internal Medicine Service. Instituto de Investigación Hospital 12 de Octubre (I+12), Madrid, España
5 La Paz Hospital Research Institute (Idipaz), Madrid, España
6 Hepatic Unit, Hospital Ramos Mejía, Buenos Aires, Argentina
7 Alfonso X El Sabio University, Villanueva de la Cañada, Madrid, España
Read
179
Timeswas read the article
61
Total PDF
118
Total HTML
Share statistics
array:22 [ "pii" => "S1665268123000169" "issn" => "16652681" "doi" => "10.1016/j.aohep.2023.100912" "estado" => "S300" "fechaPublicacion" => "2023-03-01" "aid" => "100904.8" "copyrightAnyo" => "2023" "documento" => "simple-article" "crossmark" => 0 "subdocumento" => "abs" "cita" => "Ann Hepatol. 2023;28 Supl 1:" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:1 [ "total" => 0 ] "itemSiguiente" => array:17 [ "pii" => "S1665268123000170" "issn" => "16652681" "doi" => "10.1016/j.aohep.2023.100913" "estado" => "S300" "fechaPublicacion" => "2023-03-01" "aid" => "100904.9" "documento" => "simple-article" "crossmark" => 0 "subdocumento" => "abs" "cita" => "Ann Hepatol. 2023;28 Supl 1:" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:1 [ "total" => 0 ] "en" => array:9 [ "idiomaDefecto" => true "titulo" => "P- 9 BIOMARKERS OF THE BACTERIAL, VIRAL AND HUMAN GUT TRANSCRIPTOME IN METABOLIC ASSOCIATED FATTY LIVER DISEASE (MAFLD) IN ARGENTINA" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => "en" "contieneResumen" => array:1 [ "en" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "María Florencia Mascardi, Flavia Noelia Mazzini, Bárbara Suárez, Vera M. Ruda, Sebastián Marciano, Paola Casciato, Adrián Narvaez, Leila Haddad, Margarita Anders, Federico Orozco, Ana Jesica Tamaroff, Frank Cook, John Gounarides, Susana Gutt, Adrián Gadano, Celia Méndez García, Martín L. Marro, Alberto Penas Steinhardt, Julieta Trinks" "autores" => array:19 [ 0 => array:2 [ "nombre" => "María Florencia" "apellidos" => "Mascardi" ] 1 => array:2 [ "nombre" => "Flavia Noelia" "apellidos" => "Mazzini" ] 2 => array:2 [ "nombre" => "Bárbara" "apellidos" => "Suárez" ] 3 => array:2 [ "nombre" => "Vera M." "apellidos" => "Ruda" ] 4 => array:2 [ "nombre" => "Sebastián" "apellidos" => "Marciano" ] 5 => array:2 [ "nombre" => "Paola" "apellidos" => "Casciato" ] 6 => array:2 [ "nombre" => "Adrián" "apellidos" => "Narvaez" ] 7 => array:2 [ "nombre" => "Leila" "apellidos" => "Haddad" ] 8 => array:2 [ "nombre" => "Margarita" "apellidos" => "Anders" ] 9 => array:2 [ "nombre" => "Federico" "apellidos" => "Orozco" ] 10 => array:2 [ "nombre" => "Ana Jesica" "apellidos" => "Tamaroff" ] 11 => array:2 [ "nombre" => "Frank" "apellidos" => "Cook" ] 12 => array:2 [ "nombre" => "John" "apellidos" => "Gounarides" ] 13 => array:2 [ "nombre" => "Susana" "apellidos" => "Gutt" ] 14 => array:2 [ "nombre" => "Adrián" "apellidos" => "Gadano" ] 15 => array:2 [ "nombre" => "Celia Méndez" "apellidos" => "García" ] 16 => array:2 [ "nombre" => "Martín L." "apellidos" => "Marro" ] 17 => array:2 [ "nombre" => "Alberto Penas" "apellidos" => "Steinhardt" ] 18 => array:2 [ "nombre" => "Julieta" "apellidos" => "Trinks" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1665268123000170?idApp=UINPBA00004N" "url" => "/16652681/00000028000000S1/v2_202308150516/S1665268123000170/v2_202308150516/en/main.assets" ] "itemAnterior" => array:17 [ "pii" => "S1665268123000157" "issn" => "16652681" "doi" => "10.1016/j.aohep.2023.100911" "estado" => "S300" "fechaPublicacion" => "2023-03-01" "aid" => "100904.7" "documento" => "simple-article" "crossmark" => 0 "subdocumento" => "abs" "cita" => "Ann Hepatol. 2023;28 Supl 1:" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:1 [ "total" => 0 ] "en" => array:9 [ "idiomaDefecto" => true "titulo" => "P-7 LIVER INJURY AFTER COVID-19 VACCINATION COMPARED TO POST-INFLUENZA VACCINES: RETROSPECTIVE COHORT STUDY" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => "en" "contieneResumen" => array:1 [ "en" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Marlene Padilla Lopez, Natalia Sobenko, Valeria Ines Aliperti, Vanina Cecilia Stanek, Maria Florencia Grande Ratti, Fernando Ezequiel Jabif, Marcelo Gabriel Vallone, Alejandra Villamil" "autores" => array:8 [ 0 => array:2 [ "nombre" => "Marlene Padilla" "apellidos" => "Lopez" ] 1 => array:2 [ "nombre" => "Natalia" "apellidos" => "Sobenko" ] 2 => array:2 [ "nombre" => "Valeria Ines" "apellidos" => "Aliperti" ] 3 => array:2 [ "nombre" => "Vanina Cecilia" "apellidos" => "Stanek" ] 4 => array:2 [ "nombre" => "Maria Florencia Grande" "apellidos" => "Ratti" ] 5 => array:2 [ "nombre" => "Fernando Ezequiel" "apellidos" => "Jabif" ] 6 => array:2 [ "nombre" => "Marcelo Gabriel" "apellidos" => "Vallone" ] 7 => array:2 [ "nombre" => "Alejandra" "apellidos" => "Villamil" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1665268123000157?idApp=UINPBA00004N" "url" => "/16652681/00000028000000S1/v2_202308150516/S1665268123000157/v2_202308150516/en/main.assets" ] "en" => array:10 [ "idiomaDefecto" => true "titulo" => "P-8 EXTRACELLULAR VESICLE-DERIVED MICRORNA SIGNATURE IN HCV AND HCV/HIV PATIENTS WITH DIFFERENT STAGES OF LIVER FIBROSIS" "tieneTextoCompleto" => true "autores" => array:1 [ 0 => array:3 [ "autoresLista" => "Victoria Cairoli, Daniel Valle Millares, Pablo Ryan, Lourdes Dominguez, Luz Martín-Carbonero, Beatriz Ameigeiras, Verónica Briz, Amanda Fernandez Rodriguez, Ma Victoria Preciado, Pamela Valva" "autores" => array:10 [ 0 => array:3 [ "nombre" => "Victoria" "apellidos" => "Cairoli" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">1</span>" "identificador" => "aff0001" ] ] ] 1 => array:3 [ "nombre" => "Daniel Valle" "apellidos" => "Millares" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">2</span>" "identificador" => "aff0002" ] ] ] 2 => array:3 [ "nombre" => "Pablo" "apellidos" => "Ryan" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">3</span>" "identificador" => "aff0003" ] ] ] 3 => array:3 [ "nombre" => "Lourdes" "apellidos" => "Dominguez" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">4</span>" "identificador" => "aff0004" ] ] ] 4 => array:3 [ "nombre" => "Luz" "apellidos" => "Martín-Carbonero" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">5</span>" "identificador" => "aff0005" ] ] ] 5 => array:3 [ "nombre" => "Beatriz" "apellidos" => "Ameigeiras" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">6</span>" "identificador" => "aff0006" ] ] ] 6 => array:3 [ "nombre" => "Verónica" "apellidos" => "Briz" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">2</span>" "identificador" => "aff0002" ] ] ] 7 => array:3 [ "nombre" => "Amanda Fernandez" "apellidos" => "Rodriguez" "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">2</span>" "identificador" => "aff0002" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">7</span>" "identificador" => "aff0007" ] ] ] 8 => array:3 [ "nombre" => "Ma Victoria" "apellidos" => "Preciado" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">1</span>" "identificador" => "aff0001" ] ] ] 9 => array:3 [ "nombre" => "Pamela" "apellidos" => "Valva" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">1</span>" "identificador" => "aff0001" ] ] ] ] "afiliaciones" => array:7 [ 0 => array:3 [ "entidad" => "Multidisciplinary Institute for Pediatric Pathology Research (Imipp-Conicet-Gcba), Buenos Aires, Argentina" "etiqueta" => "1" "identificador" => "aff0001" ] 1 => array:3 [ "entidad" => "National Microbiology Center, Instituto de Salud Carlos III, Madrid, España" "etiqueta" => "2" "identificador" => "aff0002" ] 2 => array:3 [ "entidad" => "Infectious Diseases Department, Hospital Universitario Infanta Leonor, Madrid, España" "etiqueta" => "3" "identificador" => "aff0003" ] 3 => array:3 [ "entidad" => "HIV Unit. Internal Medicine Service. Instituto de Investigación Hospital 12 de Octubre (I+12), Madrid, España" "etiqueta" => "4" "identificador" => "aff0004" ] 4 => array:3 [ "entidad" => "La Paz Hospital Research Institute (Idipaz), Madrid, España" "etiqueta" => "5" "identificador" => "aff0005" ] 5 => array:3 [ "entidad" => "Hepatic Unit, Hospital Ramos Mejía, Buenos Aires, Argentina" "etiqueta" => "6" "identificador" => "aff0006" ] 6 => array:3 [ "entidad" => "Alfonso X El Sabio University, Villanueva de la Cañada, Madrid, España" "etiqueta" => "7" "identificador" => "aff0007" ] ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><p id="para9001" class="elsevierStylePara elsevierViewall"><elsevierMultimedia ident="fig0001"></elsevierMultimedia></p><p id="para0005" class="elsevierStylePara elsevierViewall">Figure 1. Heatmap showing the top 50 miRNAs between patients with F<2 and F≥2 in A) HCV and B) HCV/HIV cases.</p></span>" "pdfFichero" => "main.pdf" "tienePdf" => true "tieneResumen" => true "resumen" => array:1 [ "en" => array:2 [ "resumen" => "<span id="abss0001" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0001">Introduction and Objectives</span><p id="spara001" class="elsevierStyleSimplePara elsevierViewall">Extracellular vesicles (EVs) are essential players in cell communication, and their cargo modulates the receptor cell response. MicroRNAs (miRNAs) proved to modulate the immune response both in physiological and pathological conditions. Hepatitis C (HCV) and Human Immunodeficiency (HIV) virus infection could modify EVs miRNA content and, therefore, the immune response. This study aimed to analyze the significant differentially expressed (SDE) EVs-derived miRNAs between HCV and HCV/HIV-infected patients, analyze differences according to liver fibrosis stages and explore the associated molecular pathways.</p></span> <span id="abss0002" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0002">Materials and Methods</span><p id="spara002" class="elsevierStyleSimplePara elsevierViewall">Plasma from 21 chronic HCV and 29 HCV/HIV patients were analyzed. EVs were isolated and total EV-containing RNA enriched with small RNAs was high-throughput sequenced (1 × 50). Raw reads were analyzed with Fastqc and trimmed with Cutadapt. Human-miRNA identification was performed with miRDeep2. R package edgeR was used to detect SDE miRNAs between groups and <span class="elsevierStyleItalic">in silico</span> miRNA target prediction was performed with DIANA-mirPath.</p></span> <span id="abss0003" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0003">Results</span><p id="spara003" class="elsevierStyleSimplePara elsevierViewall">HCV patients [54 years (46.5; 62.5), 52.4% F≥2] showed 38 SDE miRNAs compared with the HCV/HIV group [50 years (45; 53), 22.58 % F≥2] that modulate pathways related to fatty acids biosynthesis, extracellular matrix interaction and viral carcinogenesis. Regarding fibrosis, HCV patients with F<2 showed downregulation of hsa-miR-3615 (log<span class="elsevierStyleInf">2</span>FC=-0.92, p=0.039), which modulates genes involved in the cell cycle and the mRNA surveillance pathway. On the other hand, HCV/HIV patients with F<2 had 13 SDE miRNAs compared with F≥2. Among them, hsa-miR-122-5p (downregulated) and hsa-miR-328-3p (upregulated) showed the most significant differences (log<span class="elsevierStyleInf">2</span>FC=-1.22, p=0.034, log2FC=1.33, p=0.042, respectively). Together, they regulate genes involved in cancer-related pathways and fatty acid metabolism.</p></span> <span id="abss0004" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0004">Conclusions</span><p id="spara004" class="elsevierStyleSimplePara elsevierViewall">Differentially expressed EVs-derived miRNAs in HCV and HCV/HIV chronic infection and in different stages of liver fibrosis were observed. The specific miRNA signature of each liver fibrosis stage may elucidate potential mechanisms involved in the clinical evolution of these patients and the identification of biomarkers of unfavorable progression.</p></span>" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abss0001" "titulo" => "Introduction and Objectives" ] 1 => array:2 [ "identificador" => "abss0002" "titulo" => "Materials and Methods" ] 2 => array:2 [ "identificador" => "abss0003" "titulo" => "Results" ] 3 => array:2 [ "identificador" => "abss0004" "titulo" => "Conclusions" ] ] ] ] "multimedia" => array:1 [ 0 => array:6 [ "identificador" => "fig0001" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => false "mostrarDisplay" => true "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr4.jpeg" "Alto" => 2904 "Ancho" => 1591 "Tamanyo" => 467110 ] ] "detalles" => array:1 [ 0 => array:3 [ "identificador" => "alt0001" "detalle" => "Unlabelled imag" "rol" => "short" ] ] ] ] ] "idiomaDefecto" => "en" "url" => "/16652681/00000028000000S1/v2_202308150516/S1665268123000169/v2_202308150516/en/main.assets" "Apartado" => null "PDF" => "https://static.elsevier.es/multimedia/16652681/00000028000000S1/v2_202308150516/S1665268123000169/v2_202308150516/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1665268123000169?idApp=UINPBA00004N" ]
| Year/Month | Html | Total | |
|---|---|---|---|
| 2024 October | 3 | 2 | 5 |
| 2024 September | 6 | 2 | 8 |
| 2024 August | 5 | 4 | 9 |
| 2024 July | 5 | 3 | 8 |
| 2024 June | 6 | 1 | 7 |
| 2024 May | 9 | 3 | 12 |
| 2024 April | 11 | 2 | 13 |
| 2024 March | 5 | 2 | 7 |
| 2024 February | 12 | 6 | 18 |
| 2024 January | 10 | 6 | 16 |
| 2023 December | 5 | 8 | 13 |
| 2023 November | 7 | 2 | 9 |
| 2023 October | 7 | 7 | 14 |
| 2023 September | 7 | 2 | 9 |
| 2023 August | 6 | 0 | 6 |
| 2023 July | 2 | 10 | 12 |
| 2023 June | 4 | 1 | 5 |
| 2023 May | 7 | 0 | 7 |
| 2023 March | 1 | 0 | 1 |
Show all
