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        "resumen" => "<span id="abss0001" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0001">Introduction and Objectives</span><p id="spara001" class="elsevierStyleSimplePara elsevierViewall">To achieve WHO&#39;s goal of eliminating HCV&#44; innovative strategies must be designed to diagnose and treat more patients&#46; This study aimed to describe an implementation strategy to identify patients with HCV who were lost to follow-up &#40;LTFU&#41; and offer them re-linkage to Figure 1&#46; Analysis of global DNA methylation&#46; A&#41; Representative dot blot using anti-5mC which recognizes global methylated DNA&#44; anti-IgG as negative control and methylene blue staining as total DNA loading control&#46; B&#41; Graphs shows mean &#177; standard deviation of 5mC densitometry brand intensity of study groups&#46; C&#41; Graph that represents the percentage of global methylation of the DNA analyzed with ELISA&#46;A one-way ANOVA statistical test and a Tukey post hoc test were performed&#46; Group NT&#58; only received vehicle&#59; Group HCC&#58; damage group induced by weekly administration of DEN and 2-AAF for 12 weeks&#59; and Group HCC&#47;PFD&#58; which received the same treatment as Group HCC&#44; plus PFD &#40;300 mg&#47;kg&#41; &#40;&#42;&#42;p&#60;0&#46;005&#41;Figure 1&#46; Analysis of global DNA methylation&#46; A&#41; Representative dot blot using anti-5mC which recognizes global methylated DNA&#44; anti-IgG as negative control and methylene blue staining as total DNA loading control&#46; B&#41; Graphs shows mean &#177; standard deviation of 5mC densitometry brand intensity of study groups&#46; C&#41; Graph that represents the percentage of global methylation of the DNA analyzed with ELISA&#46;A one-way ANOVA statistical test and a Tukey post hoc test were performed&#46; Group NT&#58; only received vehicle&#59; Group HCC&#58; damage group induced by weekly administration of DEN and 2-AAF for 12 weeks&#59; and Group HCC&#47;PFD&#58; which received the same treatment as Group HCC&#44; plus PFD &#40;300 mg&#47;kg&#41; &#40;&#42;&#42;p&#60;0&#46;005&#41;Figure 1&#46; Analysis of global DNA methylation&#46; A&#41; Representative dot blot using anti-5mC which recognizes global methylated DNA&#44; anti-IgG as negative control and methylene blue staining as total DNA loading control&#46; B&#41; Graphs shows mean &#177; standard deviation of 5mC densitometry brand intensity of study groups&#46; C&#41; Graph that represents the percentage of global methylation of the DNA analyzed with ELISA&#46;A one-way ANOVA statistical test and a Tukey post hoc test were performed&#46; Group NT&#58; only received vehicle&#59; Group HCC&#58; damage group induced by weekly administration of DEN and 2-AAF for 12 weeks&#59; and Group HCC&#47;PFD&#58; which received the same treatment as Group HCC&#44; plus PFD &#40;300 mg&#47;kg&#41; &#40;&#42;&#42;p&#60;0&#46;005&#41;</p></span> <span id="abss0002" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0002">Materials and Methods</span><p id="spara002" class="elsevierStyleSimplePara elsevierViewall">We conducted an implementation study utilizing a strategy to contact patients with HCV who were not under regular follow-up in 45 centers from 13 Latin American countries&#46; Patients with HCV were identified by the international classification of diseases &#40;ICD-9&#47;10&#41; or equivalent&#46; Medical records were then reviewed to confirm the diagnosis of chronic HCV infection defined by anti-HCV&#43; and detectable HCV-RNA&#46; Identified patients who were not under follow-up by a liver specialist were contacted by telephone or email and offered a medical reevaluation&#46;</p></span> <span id="abss0003" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0003">Results</span><p id="spara003" class="elsevierStyleSimplePara elsevierViewall">A total of 10364 patients were classified to have HCV&#46; After reviewing their medical charts&#44; 1349 &#40;13&#37;&#41; had undetectable HCV-RNA or were wrongly coded &#40;figure&#41;&#46; Overall&#44; 9015 &#40;86&#46;9&#37;&#41; individuals were identified with chronic HCV infection&#46; A total of 5096 &#40;56&#46;5&#37;&#41; patients were under routine HCV care and 3919 &#40;43&#46;5&#37;&#41; had been LTFU&#46; We were able to contact 1617 &#40;41&#46;3&#37;&#41; of the 3919 patients who were LTFU at the primary medical institution&#44; of which 427 &#40;26&#46;4&#37;&#41; were cured at different institutions or were dead&#46; Of the remaining patients&#44; 906 &#40;76&#46;1&#37;&#41; were candidates for retrieval&#46; Overall&#44; patients who were LTFU were younger &#40;58&#46;7 vs&#46; 61&#46;1 years&#59; p&#60;0&#46;001&#41;&#44; were more likely to be men &#40;57&#46;4&#37; vs&#46; 49&#46;5&#37;&#59; p&#60;0&#46;001&#41;&#44; and to have a concomitant infection of HIV &#40;13&#46;8&#37; vs&#46; 7&#46;3&#37;&#59; p&#60;0&#46;001&#41; and HBV &#40;3&#46;1&#37; vs&#46; 1&#46;7&#37;&#59; p&#60;0&#46;001&#41;&#46;</p></span> <span id="abss0004" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0004">Conclusions</span><p id="spara004" class="elsevierStyleSimplePara elsevierViewall">In our cohort&#44; about 1 out of 4 patients with chronic HCV who were LTFU were candidates to receive treatment&#46; This strategy has the potential to be effective and accessible and significantly impacts the HCV care cascade&#46; &#40;NCT04470271&#41;</p></span>"
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