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      "titulo" => "Hepatitis C virus-infected patients carriers of the TT &#40;C&#42;52T&#44; rs14158&#41; genotype of the <span class="elsevierStyleItalic">LDL</span> receptor and <span class="elsevierStyleItalic">Apo3</span> present severe liver damage in West Mexico"
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      "titulo" => "The use of molecular adsorbent recirculating system and single-pass albumin dialysis as liver support&#58; The experience of the Centro Medico Nacional 20 de Noviembre"
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    "titulo" => "Hepatitis C Virus NS5A and Core protein induce hepatic stellate cells activation promoting fibrosis-related gene regulation on hepatocytes&#46;"
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        "autoresLista" => "Tania G&#46; Heredia-Torres, Sonia A&#46; Lozano-Sep&#250;lveda, Ana R&#46; Rinc&#243;n-S&#225;nchez, Ana M&#46; Guadalupe Rivas-Estilla"
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            "entidad" => "Departamento de Bioqu&#237;mica y Medicina Molecular&#44; Centro de Investigaci&#243;n e Innovaci&#243;n en Virolog&#237;a M&#233;dicaFacultad de Medicina&#44; Universidad Aut&#243;noma de Nuevo Le&#243;n&#44; Monterrey&#44; Nuevo Le&#243;n&#44; Mexico"
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            "entidad" => "IBMMTG&#44; Departamento de Biolog&#237;a Molecular y Gen&#243;mica&#44; Centro Universitario de Ciencias de la Salud&#44; Universidad de Guadalajara&#44; Guadalajara&#44; Jalisco&#44; M&#233;xico"
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    "textoCompleto" => "<span class="elsevierStyleSections"><p id="para0005" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Ethical statement</span></p><p id="para0006" class="elsevierStylePara elsevierViewall">The protocol was registered and approved by the Ethics Committee&#46;</p><p id="para0007" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Declaration of interests</span></p><p id="para0008" class="elsevierStylePara elsevierViewall">None</p><p id="para0009" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Funding</span></p><p id="para0010" class="elsevierStylePara elsevierViewall">The study was funded by the Department of Biochemistry and Molecular Medicine and CIIViM&#44; School of Medicine&#44; and by PAICYT 171-CS-2022 from Universidad Aut&#243;noma de Nuevo Le&#243;n &#40;UANL&#41;&#44; Monterrey&#44; Nuevo Le&#243;n 64460&#44; Mexico&#46;</p></span>"
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        "resumen" => "<span id="abss0001" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0001">Introduction and Objectives</span><p id="spara001" class="elsevierStyleSimplePara elsevierViewall">Chronic HCV infection leads to the development of liver fibrosis mediated by intercellular communication between hepatocytes and hepatic stellate cells &#40;HSCs&#41;&#46; The diverse molecular pathways involved in the development of fibrosis in hepatocytes derived from HSC activation induced by viral proteins remain to be fully determined&#46; Our aim is to determine differentially expressed genes associated with fibrotic processes in hepatocytes &#40;Huh7&#41; that express the HCV NS5A or Core protein during co-culture with HSC &#40;LX2&#41;&#46;</p></span> <span id="abss0002" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0002">Materials and methods</span><p id="spara002" class="elsevierStyleSimplePara elsevierViewall">Huh7 cells were transfected to express NS5A or Core proteins and co-cultured with HSC-LX2 cells&#46; Viral protein expression and expression of TGF&#946;1&#44; Col1&#44; and aSMA was determined to assess LX2 activation&#46; A profile of 84 genes associated with fibrosis during co-cultivation was determined and analyzed&#46;</p></span> <span id="abss0003" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0003">Results</span><p id="spara003" class="elsevierStyleSimplePara elsevierViewall">HSC-LX2 co-cultured with transfected Huh7 showed an 8&#46;3&#44; 6&#46;7 and 4-fold increase in collagen1&#44; TGFB1 and timp1 expression respectively induced by NS5A and a 6&#46;5&#44; 1&#46;8 and 6&#46;2-fold increase respectively induced by Core&#44; all these compared to HSC-LX2 co-cultured with untransfected Huh7&#46; We detected 28 overexpressed genes in Huh7 &#40;NS5A&#43;&#41; and 46 differentially expressed genes in Huh7 &#40;Core&#43;&#41; in co-culture with HSC-LX2&#44; compared to untransfected Huh7 in co-culture with HSC-LX2&#46; Analysis of the expression profile showed that the TGF&#946;1&#44; the ECM regulation&#44; and growth factors pathways are the molecular mechanisms involved during the co-culture of Huh7 transfected with NS5A or Core with HSC-LX2&#46;</p></span> <span id="abss0004" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0004">Conclusions</span><p id="spara004" class="elsevierStyleSimplePara elsevierViewall">HCV NS5A and Core proteins expression in Huh7 cells induces the HSC-LX2 activation&#44; regulating the expression of diverse genes in hepatocytes that trigger different molecular mechanisms involved in the fibrosis development&#44; this information provide the identification of possible anti-fibrotic targets drugs associated with HCV infection for further study&#46;</p></span>"
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