metricas
covid
Buscar en
Clínica e Investigación en Ginecología y Obstetricia
Toda la web
Inicio Clínica e Investigación en Ginecología y Obstetricia Análisis del factor cervical de esterilidad
Journal Information
Vol. 30. Issue 2.
Pages 53-58 (January 2003)
Share
Share
Download PDF
More article options
Vol. 30. Issue 2.
Pages 53-58 (January 2003)
Full text access
Análisis del factor cervical de esterilidad
Visits
11014
R. Rodrígueza, R. Hernándeza, L. Cabreraa, A. Fernándeza, J. Albertoa
a Facultad de Medicina.
P. Prietob
b Facultad de Psicología. Universidad de La Laguna. Hospital Universitario de Canarias. Tenerife. España.
This item has received
Article information
Summary
Objective

To investigate the role of both cervical mucus and semen parameters in diagnostic tests of the cervical factor: Insler test, postcoital test (PCT) and in vitro sperm-cervical mucus penetration test (PT), with special interest in identified pathogenic organisms.

Material and methods

We investigated infertility in 376 infertile patients, concentrating on the cervical factor in 281 of them (74.73%), and using three test methods. Insler (n = 201), postcoital (n = 254), and PT (n = 79), and endocervical and vaginal secretion cultures in all of them. Also the direct microimmuno-fluorescence test (Micro-Trak)®and 222 routine sperm tests on their partners.

Results

Insler test was normal in 72.6% of the cases, without significant (NS) correlation with infection. The postcoital test was pathological in 72.55% of cases. We correlated these with the Insler test results (P< 0.005; χ21 = 105.670), TPV results (NS), semen parameters (P < 0.005; χ21 = 4.441) and infection (NS), particularly with Chlamydia trachomatis21= 0.529; P< 0.005). The PT showed in 45.8% of cases the sperm did not penetrate, in 24.1% it did, in 3.6% mobility was lowered, and in 26.5% it was immobile, noting that PT was in correlation with sperm analysis (P < 0.005; χ21= 3.920).

Conclusion:

The results of the postcoital test depend on the properties of the mucus and semen, these being altered by the presence of C. trachomatisinfection, so that PT is related to the sperm parameters.

Resumen
Objetivo:

Se ha evaluado la influencia tanto del moco cervical como de las características del semen en las diversas pruebas diagnósticas del factor cervical: test de Insler,test poscoital y test de penetración in vitro,con especial atención al papel que desempeñan los microorganismos patógenos identificados.

Material y métodos:

Se efectuó el protocolo de esterilidad a un total de 376 mujeres,centrándonos en el análisis del factor cervical,llevado a cabo en 281 de ellas (74,73%), con tres pruebas:de Insler (n = 201), poscoital (n = 254) y de penetración in vitro (n = 79),además de cultivos de la secreción endocervical y vaginal a todas ellas, y test de microinmunofluorescencia directa para la identificación de Chlamy dia trachomatis (Micro-Trak®),así como 222 espermiogramas a sus parejas.

Resultados:

El test de Insler fue normal en el 72,6% de los casos,no hallando relación con la infección (NS). El test poscoital se estableció como patoló gicoen el 72,5% de los casos. Se relacionaron sus resultados con el test de Insler (p < 0,005; χ21= 5,670), el test de penetración in vitro (NS),con los del espermiograma (p < 0,005; χ21= 4,441) y con la infección demanera global (NS) y por C. trachomatisen particular(χ21= 0,529; p < 0,005). En el test de penetración in vitro se observó que en un 45,8% de los casos los espermatozoides no penetraban,en un 24,1% sí lo hacían,en tanto que en un 3,6% se disminuía su movilidad y en un 26,5% se inmovilizaban,observando que el test de penetración in vitro se relacionaba con el espermiograma (p < 0,005:χ21 = 3,920).

Conclusión:

Los resultados del test poscoital dependen de las características del moco y del semen, y se encuentran alterados ante la presencia de infección por C. trachomatis,en tanto que el test de penetración in vitro se relaciona con los valores del espermiograma.

Full text is only aviable in PDF
Bibliografía
[1.]
W. Eggert-Kruse, A. Hofsäß, E. Haury, W. Tilgen, I. Runnenbaum R. Gerhard.
Relationship between local anti-sperm antibodies and sperm-mucus interaction in vitroand in vivo.
Hum Reprod, 6 (1991), pp. 267-276
[2.]
A. Beltsos, S. Fisher, M. Uhler, E. Clegg, M. Zinaman.
The relationship of the postcoital test and semen characteristics to pregnancy rates in 200 presumed fertile couples.
Int J Fertil Menopausal Stud, 41 (1996), pp. 405-411
[3.]
E. Barnea, R. McInnes.
Reappraisal of the postcoital test: A controlled study.
Int J Fertil, 31 (1996), pp. 46-49
[4.]
V. Annapurna, L.K. Dhaliwal, S. Gopalan.
Effect of two anti-estrogens, clomiphene citrate and tamoxifen, on cervical mucus and sperm-cervical mucus interaction.
Int J Fertil Womens Med, 42 (1997), pp. 225-228
[5.]
M.R. Bush, D.K. Walmer, G.M. Couchman, A.F. Haney.
Evaluation of the postcoital test in cycles involving exogenous gonadotropins.
Obstet Gynecol, 89 (1997), pp. 780-784
[6.]
C.S. Griffith, D.A. Grimes.
The validity of the postcoital test.
Am J Obstet Gynecol, 162 (1990), pp. 615-620
[7.]
I. Glatstein, C.L. Best, A. Palumbo, L.A. Sleeper, A.J. Friedman, M. Hornstein.
The reproductive of the postcoital test: a prospective study.
Obst Gynecol, 85 (1995), pp. 396-400
[8.]
M. Seibel.
Work up of the infertile couple.
Infertility: a comprehensive text, pp. 10-12
[9.]
S. Guid Oei, K. Bloemenkamp, F.M. Helmerhorst, N. Naaktgeboren, H. Keirse.
Evaluation of the postcoital test for assessment of «cervical factors» infertility.
Eur J Obstet Gynecol Reprod Biol, 64 (1996), pp. 217-220
[10.]
J. Belaisch.
Que reste-t-il du test post-coital?.
Contracept Fertil Sex, 27 (1999), pp. 823-825
[11.]
S. Guid Oei, M. Keirse, K. Bloemenkamp, F.M. Helmerhorst.
European postcoital test: opinions and practice.
Br J Obst Gynaecol, 102 (1995), pp. 621-624
[12.]
A. Johnson, B. Bassham, L.I. Lipshultz, D.J. Lamb.
A quality control system for the optimized sperm penetration assay.
Fertil Steril, 64 (1995), pp. 832-837
[13.]
J. Farhi, G. Bahadur, F. Henfield, S.J. Steele, H.S. Jacobs.
In vitro cervical mucus sperm penetration tests and outcome of infertility treatments in couples with repeatedly negative postcoital tests.
Hum Reprod, 10 (1995), pp. 85-90
[14.]
R. Rodríguez, R. Hernández, F. Fuster, A. Torres, P. Prieto, J. Alberto.
Infección genital y esterilidad.
Enferm Infecc Microbiol Clin, 19 (2001), pp. 261-266
[15.]
C. Wang, G. Waites, E. Neischlag.
The World Health Organization laboratory manual for the examination of human semen and sperm-cervical mucus interaction.
[16.]
K.S. Moghissi.
Postcoital test: phisiologic basis, technique and interpretation.
Fertil Steril, 27 (1976), pp. 117-129
[17.]
American Fertility Society.
Investigation of the infertile couple.
[18.]
E. Moreno Pacha, F. Sánchez, J.L. Moliní.
Metodología diagnóstica y terapéutica de la interacción mocosemen.
Act Reprod Hum, 1 (1996), pp. 2-14
[19.]
W.D. Schalaff, J.A. Rock.
Decision making in reproductive en-docrinology.
[20.]
W. Eggert-Kruse, A. Köhler, G. Rohr, B. Runnebaum.
The pH as an important determinant of sperm-mucus interaction.
Fertil Steril, 59 (1993), pp. 617-628
[21.]
S. Guid Oei, F.M. Helmerhorst, K.W.M. Bloemenkamp, M. Dersjant-Rooarda, M. Keirse.
Predicting optimal cervical mucus for infertility diagnosis.
Eur J Obst Gynecol Reprod Biol, 73 (1997), pp. 63-66
[22.]
D.R. Franken, S. Pretorius, S. Globler, J.I. De Wet.
Important semen parameters during postcoital testing.
Arch Androl, 14 (1985), pp. 213-215
[23.]
J. Kremer, S. Jager, T. Van Slochteren-Draaisma.
The «unex-plained», poor post coital test.
Int J Fertil, 23 (1978), pp. 277-281
[24.]
F. Lombardo, L. Gandini, F. Dondero, A. Lenzi.
Antisperm immunitiy in natural and assisted reproduction.
Hum Reprod Update, 5 (2001), pp. 450-456
[25.]
Eggert-Kruse W, Doll A, Böckem S, Runnenbaum B. Evaluation of cervical mucus antibodies with indirect mixed antiglobulin reaction (MAR) test. Kongres proceedings, Dreiländertagung Fertilität und Sterilität, Goslar, Alete Wiss. Dienst 1991;283-4
[26.]
Ten Brug CS, De Jogh-Mulders M, Vermeiden JPW, Bernardus RE, Schoemaker J. Pregnancy rate and incidence of agglutinating antisperm antibodies (Ag-ASA): a retrospective study. In: Human Reproduction: abstract of the 10th Annual Meeting of the ESHRE, 1994; p. 97
[27.]
J. Núñez-Troconis.
Mycoplasma hominisy Ureaplasma ure-alyticumen diferentes patologías ginecológicas.
Invest Clin, 40 (1999), pp. 9-24
[28.]
W. Eggert-Kruse, S. Pohl, H. Näher, W. Tilgen, B. Runnebaum.
Microbial colonization and sperm-mucus interaction: results in 1000 infertile couples.
Hum Reprod, 7 (1992), pp. 612-620
[29.]
D.R. Tredway, JW Jr Wortham, M. Condon-Mahony, D. Baker, J.M. Shane.
Correlation of postcoital evaluation with in vitro sperm cervical mucus determinations and Ureaplasmacultures.
Fertil Steril, 43 (1985), pp. 286-289
Copyright © 2003. Elsevier España, S.L.. Todos los derechos reservados
Download PDF
Article options
es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos