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Nissl staining indicated that rat cortical cell numbers in the VF + PBS group were significantly lower than in the VF + PBS group 72 h after ROSC (A, B). There was a greater number of TUNEL-positive neurons (indicated by the white arrow) in rat cortices in the VF + PBS group than in the VF + UTI group 72 h after ROSC (C, D) (72 standard fields in the VF + PBS group and 126 standard fields in the VF+UTI group were analyzed). ∗ VF + PBS <span class="elsevierStyleItalic">versus</span> VF + UTI, <span class="elsevierStyleItalic">p</span><0.05.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Chun Lin Hu, Jin Ming Xia, Jie Cai, Xin Li, Xiao Xing Liao, Hui Li, Hong Zhan, Gang Dai, Xiao Li Jing" "autores" => array:9 [ 0 => array:2 [ "nombre" => "Chun Lin" "apellidos" => "Hu" ] 1 => array:2 [ "nombre" => "Jin Ming" "apellidos" => "Xia" ] 2 => array:2 [ "nombre" => "Jie" "apellidos" => "Cai" ] 3 => array:2 [ "nombre" => "Xin" "apellidos" => "Li" ] 4 => array:2 [ "nombre" => "Xiao Xing" "apellidos" => "Liao" ] 5 => array:2 [ "nombre" => "Hui" "apellidos" => "Li" ] 6 => array:2 [ "nombre" => "Hong" "apellidos" => "Zhan" ] 7 => array:2 [ "nombre" => "Gang" "apellidos" => "Dai" ] 8 => array:2 [ "nombre" => "Xiao Li" "apellidos" => "Jing" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1807593222016271?idApp=UINPBA00004N" "url" => "/18075932/0000006800000009/v1_202212010704/S1807593222016271/v1_202212010704/en/main.assets" ] 1 => array:19 [ "pii" => "S1807593222016477" "issn" => "18075932" "doi" => "10.6061/clinics/2013(08)11" "estado" => "S300" "fechaPublicacion" => "2013-08-01" "aid" => "1647" "copyright" => "CLINICS" "documento" => "article" "crossmark" => 0 "licencia" => "https://creativecommons.org/licenses/by-nc/3.0/" "subdocumento" => "fla" "cita" => "Clinics. 2013;68:1128-33" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:1 [ "total" => 0 ] "en" => array:12 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">CLINICAL SCIENCE</span>" "titulo" => "Colonization pressure as a risk factor for colonization by multiresistant <span class="elsevierStyleItalic">Acinetobacter</span> spp and carbapenem-resistant <span class="elsevierStyleItalic">Pseudomonas aeruginosa</span> in an intensive care unit" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "1128" "paginaFinal" => "1133" ] ] "contieneResumen" => array:1 [ "en" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig1" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 714 "Ancho" => 796 "Tamanyo" => 31637 ] ] "descripcion" => array:1 [ "en" => "<p id="spara10" class="elsevierStyleSimplePara elsevierViewall">Kaplan-Meier curves indicating the probability of not becoming colonized by carbapenem-resistant <span class="elsevierStyleItalic">P. aeruginosa</span> and/or multiresistant <span class="elsevierStyleItalic">Acinetobacter</span> spp. during each of the three study periods. Neurology Intensive Care Unit, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil, April 2000 to July 2002.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Mirian Freitas DalBen, Mariusa Basso, Cilmara Polido Garcia, Silvia Figueiredo Costa, Cristiana Maria Toscano, William Robert Jarvis, Renata Desordi Lobo, Maura Salaroli Oliveira, Anna Sara Levin" "autores" => array:9 [ 0 => array:2 [ "nombre" => "Mirian Freitas" "apellidos" => "DalBen" ] 1 => array:2 [ "nombre" => "Mariusa" "apellidos" => "Basso" ] 2 => array:2 [ "nombre" => "Cilmara Polido" "apellidos" => "Garcia" ] 3 => array:2 [ "nombre" => "Silvia" "apellidos" => "Figueiredo Costa" ] 4 => array:2 [ "nombre" => "Cristiana" "apellidos" => "Maria Toscano" ] 5 => array:2 [ "nombre" => "William" "apellidos" => "Robert Jarvis" ] 6 => array:2 [ "nombre" => "Renata Desordi" "apellidos" => "Lobo" ] 7 => array:2 [ "nombre" => "Maura Salaroli" "apellidos" => "Oliveira" ] 8 => array:2 [ "nombre" => "Anna Sara" "apellidos" => "Levin" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1807593222016477?idApp=UINPBA00004N" "url" => "/18075932/0000006800000008/v1_202212010718/S1807593222016477/v1_202212010718/en/main.assets" ] 2 => array:19 [ "pii" => "S1807593222017999" "issn" => "18075932" "doi" => "10.6061/clinics/2012(06)14" "estado" => "S300" "fechaPublicacion" => "2012-06-01" "aid" => "1799" "copyright" => "CLINICS" "documento" => "article" "crossmark" => 0 "licencia" => "https://creativecommons.org/licenses/by-nc/3.0/" "subdocumento" => "fla" "cita" => "Clinics. 2012;67:629-37" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:1 [ "total" => 0 ] "en" => array:12 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">BASIC RESEARCH</span>" "titulo" => "<span class="elsevierStyleItalic">In vitro</span> comparative study of white and dark polycaprolactone trifumarate <span class="elsevierStyleItalic">in situ</span> cross-linkable scaffolds seeded with rat bone marrow stromal cells" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "629" "paginaFinal" => "637" ] ] "contieneResumen" => array:1 [ "en" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig5" "etiqueta" => "Figure 5" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr5.jpeg" "Alto" => 568 "Ancho" => 781 "Tamanyo" => 118717 ] ] "descripcion" => array:1 [ "en" => "<p id="spara50" class="elsevierStyleSimplePara elsevierViewall">SEM analysis of unseeded (A and B), 1 day after MSC seeding (C and D) and 14 days after MSC seeding (E and F) white and dark PCLTF scaffolds.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Kama Bistari Muhammad, Wan Abu Bakar Wan Abas, Kah Hwi Kim, Belinda Pingguan-Murphy, Norita Mohd Zain, Haris Akram" "autores" => array:6 [ 0 => array:2 [ "nombre" => "Kama Bistari" "apellidos" => "Muhammad" ] 1 => array:2 [ "nombre" => "Wan Abu Bakar Wan" "apellidos" => "Abas" ] 2 => array:2 [ "nombre" => "Kah Hwi" "apellidos" => "Kim" ] 3 => array:2 [ "nombre" => "Belinda" "apellidos" => "Pingguan-Murphy" ] 4 => array:2 [ "nombre" => "Norita Mohd" "apellidos" => "Zain" ] 5 => array:2 [ "nombre" => "Haris" "apellidos" => "Akram" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1807593222017999?idApp=UINPBA00004N" "url" => "/18075932/0000006700000006/v1_202212011548/S1807593222017999/v1_202212011548/en/main.assets" ] ] "en" => array:11 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">ERRATA</span>" "titulo" => "ERRATA" "tieneTextoCompleto" => true "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "1559" "paginaFinal" => "1560" ] ] "textoCompleto" => "<span class="elsevierStyleSections"><span id="cesec10" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle10">CLINICS 2013;68(9):1231-8</span><p id="para10" class="elsevierStylePara elsevierViewall">Xiao Li Jing is the corresponding author.<elsevierMultimedia ident="fig1"></elsevierMultimedia></p></span><span id="cesec20" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle20">CLINICS 2013;68(8):1128-33</span><span id="cesec30" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle30"><span class="elsevierStyleBold">Page 1132</span></span><p id="para20" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Replace</span> ACKNOWLEDGMENTS This study was supported by the Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil, and by the U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA. The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention or the Agency for Toxic Substances and Disease Registry.</p><p id="para30" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">For</span> ACKNOWLEDGMENTS This study was supported by the Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, Brazil, by FAPESP (grant # 2013/08308-4), and by the U.S. Centers for Disease Control and Prevention, Atlanta, GA, USA. The findings and conclusions in this report are those of the authors and do not necessarily represent the official position of the Centers for Disease Control and Prevention or the Agency for Toxic Substances and Disease Registry.</p></span></span><span id="cesec40" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle40">CLINICS 2012;67(6):629-37</span><span id="cesec50" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle50"><span class="elsevierStyleBold">Page 629 (Abstract)</span></span><p id="para40" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Replace</span> RESULTS: The degradation of the white scaffold was significantly lower compared with the dark scaffold but was within the acceptable time range for bone-healing processes. The deoxyribonucleic acid and collagen contents increased up to day 28 with no significant difference between the two scaffolds, but the glycosaminoglycan content was slightly higher in the white scaffold throughout 14 days of incubation. Scanning electron microscopy at days 1 and 14 revealed cellular growth and attachment.</p><p id="para50" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">For</span> RESULTS: The degradation of the white scaffold was significantly lower compared with the dark scaffold but was within the acceptable time range for bone-healing processes. The deoxyribonucleic acid and collagen contents increased up to day 28 with no significant difference between the two scaffolds, but the glycosaminoglycan content was slightly higher in the white scaffold throughout 14 days of incubation. Scanning electron microscopy at day 1 revealed cellular growth and attachment.</p></span><span id="cesec60" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle60"><span class="elsevierStyleBold">Page 631 (Scanning Electron Microscopy (SEM))</span></span><p id="para60" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Replace</span> Samples of unseeded and seeded (day 1 and 14) white and dark PCLTF scaffolds were processed for SEM. The samples were fixed in 4% glutaraldehyde followed by 1% osmium tetroxide and dehydrated with an ethanol series to 100% before being gold coated. The images of BMSC attachments and cell interactions between each group were compared.</p><p id="para70" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">For</span> Samples of unseeded and seeded (day 1) white and dark PCLTF scaffolds were processed for SEM. The samples were fixed in 4% glutaraldehyde followed by 1% osmium tetroxide and dehydrated with an ethanol series to 100% before being gold coated. The images of BMSC attachments and cell interactions between each group were compared.</p></span><span id="cesec70" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle70"><span class="elsevierStyleBold">Page 634-5 (Scanning Electron Microscopy (SEM))</span></span><p id="para80" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Replace</span> The interaction of BMSCs with both PCLTF scaffolds at days 1 and 14 was qualitatively characterized using SEM. The interaction test shows no advantage between white and dark PCLTF scaffolds at these time points. The unseeded scaffolds (both white and dark) have interconnected micropores, as shown in Figure 5A and 5B. The seeded BMSCs attached firmly to the scaffold surface by day 1 after incubation (Figures 5C and D). By day 14 after incubation, a well-formed cellular layer with active extracellular matrix secretion was visible (Figures 5E and F).</p><p id="para90" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">For</span> The interaction of BMSCs with both PCLTF scaffolds at day 1 after seeding was qualitatively characterized using SEM. The interaction test shows no advantage between white and dark PCLTF scaffolds at these time points. The unseeded scaffolds (both white and dark) have interconnected micropores, as shown in Figure 5A and 5B. The seeded BMSCs attached firmly to the scaffold surface by day 1 after incubation (Figures 5C and D).</p></span><span id="cesec80" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle80"><span class="elsevierStyleBold">Page 635</span></span><p id="para100" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Replace</span> Figure 5</p><p id="para110" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">For</span></p></span></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:3 [ 0 => array:2 [ "identificador" => "cesec10" "titulo" => "CLINICS 2013;68(9):1231-8" ] 1 => array:3 [ "identificador" => "cesec20" "titulo" => "CLINICS 2013;68(8):1128-33" "secciones" => array:1 [ 0 => array:2 [ "identificador" => "cesec30" "titulo" => "Page 1132" ] ] ] 2 => array:3 [ "identificador" => "cesec40" "titulo" => "CLINICS 2012;67(6):629-37" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "cesec50" "titulo" => "Page 629 (Abstract)" ] 1 => array:2 [ "identificador" => "cesec60" "titulo" => "Page 631 (Scanning Electron Microscopy (SEM))" ] 2 => array:2 [ "identificador" => "cesec70" "titulo" => "Page 634-5 (Scanning Electron Microscopy (SEM))" ] 3 => array:2 [ "identificador" => "cesec80" "titulo" => "Page 635" ] ] ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "NotaPie" => array:1 [ 0 => array:1 [ "nota" => "<p class="elsevierStyleNotepara" id="cenpara10">No potential conflict of interest was reported.</p>" ] ] "multimedia" => array:1 [ 0 => array:5 [ "identificador" => "fig1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => false "mostrarDisplay" => true "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 542 "Ancho" => 748 "Tamanyo" => 112017 ] ] ] ] ] "idiomaDefecto" => "en" "url" => "/18075932/0000006800000012/v1_202212011241/S1807593222020464/v1_202212011241/en/main.assets" "Apartado" => null "PDF" => "https://static.elsevier.es/multimedia/18075932/0000006800000012/v1_202212011241/S1807593222020464/v1_202212011241/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1807593222020464?idApp=UINPBA00004N" ]
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