Original articles
Effects of physical training on the metabolic profile of rats exposed to chronic restraint stress
Carlos H.O. Reisa, Sabriny G. Manzollia, Leonardo dos Santosb,
, Ariana A. Silvaa, Ana Paula Lima-Leopoldoa, André S. Leopoldoa, Danilo S. Bocalinia
Corresponding author
a Experimental Physiology and Biochemistry Laboratory, Physical Education and Sports Center of the Universidade Federal do Espirito Santo – Campus Goiabeiras, Vitória, ES, Brazil
b Cardiac Electromechanics Laboratory, Postgraduation Program in Physiological Sciences, Health Sciences Center, Universidade Federal do Espirito Santo - Campus Maruipe, Vitoria, ES, Brazil
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(A) HPV E6 was analyzed by Real-time PCR in primary LSCC cells. (B) HPV E7 was analyzed by Real-time PCR in primary LSCC cells.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Weiquan Ding, Weiwei Cai, Haili Wang" "autores" => array:3 [ 0 => array:2 [ "nombre" => "Weiquan" "apellidos" => "Ding" ] 1 => array:2 [ "nombre" => "Weiwei" "apellidos" => "Cai" ] 2 => array:2 [ "nombre" => "Haili" "apellidos" => "Wang" ] ] ] ] "resumen" => array:1 [ 0 => array:3 [ "titulo" => "Highlights" "clase" => "author-highlights" "resumen" => "<span id="abss0001" class="elsevierStyleSection elsevierViewall"><p id="spara007" class="elsevierStyleSimplePara elsevierViewall"><ul class="elsevierStyleList" id="celist0001"><li class="elsevierStyleListItem" id="celistitem0001"><span class="elsevierStyleLabel">•</span><p id="para0001" class="elsevierStylePara elsevierViewall">Apoptosis is required for radiation sensitivity in HPV+ LSCC cells.</p></li><li class="elsevierStyleListItem" id="celistitem0002"><span class="elsevierStyleLabel">•</span><p id="para0002" class="elsevierStylePara elsevierViewall">P53 and pRB are required for radiation sensitivity of HPV+ LSCC cells.</p></li><li class="elsevierStyleListItem" id="celistitem0003"><span class="elsevierStyleLabel">•</span><p id="para0003" class="elsevierStylePara elsevierViewall">P53 and pRB are required for radiation sensitivity of HPV+ LSCC tumors <span class="elsevierStyleItalic">in vivo</span>.</p></li></ul></p></span>" ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1807593224000929?idApp=UINPBA00004N" "url" => "/18075932/000000790000000C/v123_202411010523/S1807593224000929/v123_202411010523/en/main.assets" ] "itemAnterior" => array:17 [ "pii" => "S1807593224000875" "issn" => "18075932" "doi" => "10.1016/j.clinsp.2024.100410" "estado" => "S250" "fechaPublicacion" => "2024-01-01" "aid" => "100410" "copyright" => "HCFMUSP" "documento" => "article" "crossmark" => 1 "subdocumento" => "fla" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "en" => array:12 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original articles</span>" "titulo" => "Identification of potential crucial cuproptosis-related genes in myocardial ischemia-reperfusion injury through the bioinformatic analysis" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => array:2 [ 0 => "en" 1 => "en" ] "contieneResumen" => array:1 [ "en" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:8 [ "identificador" => "fig0006" "etiqueta" => "Fig. 6" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr6.jpeg" "Alto" => 2002 "Ancho" => 3000 "Tamanyo" => 314992 ] ] "detalles" => array:1 [ 0 => array:3 [ "identificador" => "alt0006" "detalle" => "Fig " "rol" => "short" ] ] "descripcion" => array:1 [ "en" => "<p id="spara006" class="elsevierStyleSimplePara elsevierViewall">The expression and AUC value of Dlat, Pdhb, and Pdhα1 in MI/RI patients. (A) The Dlat, Pdhb, and Pdhα1 expressions in the serum samples of the ischemia-reperfusion injury patients were detected by qPCR. (B) Receiver operating characteristics curve analysis was conducted to predict the diagnostic value of Pdhb, Dlat, and Pdhα1 (** p < 0.01, *** p < 0.001).</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Rong Huang, Rongfeng Xu, Rui Zhang, Wenjie Zuo, Zhenjun Ji, Zaixiao Tao, Yongjun Li, Genshan Ma" "autores" => array:8 [ 0 => array:2 [ "nombre" => "Rong" "apellidos" => "Huang" ] 1 => array:2 [ "nombre" => "Rongfeng" "apellidos" => "Xu" ] 2 => array:2 [ "nombre" => "Rui" "apellidos" => "Zhang" ] 3 => array:2 [ "nombre" => "Wenjie" "apellidos" => "Zuo" ] 4 => array:2 [ "nombre" => "Zhenjun" "apellidos" => "Ji" ] 5 => array:2 [ "nombre" => "Zaixiao" "apellidos" => "Tao" ] 6 => array:2 [ "nombre" => "Yongjun" "apellidos" => "Li" ] 7 => array:2 [ "nombre" => "Genshan" "apellidos" => "Ma" ] ] ] ] "resumen" => array:1 [ 0 => array:3 [ "titulo" => "Highlights" "clase" => "author-highlights" "resumen" => "<span id="abss0001" class="elsevierStyleSection elsevierViewall"><p id="spara007" class="elsevierStyleSimplePara elsevierViewall"><ul class="elsevierStyleList" id="celist0001"><li class="elsevierStyleListItem" id="celistitem0001"><span class="elsevierStyleLabel">•</span><p id="para0002" class="elsevierStylePara elsevierViewall">Cuproptosis-related genes are involved in acute myocardial infarction progression.</p></li><li class="elsevierStyleListItem" id="celistitem0002"><span class="elsevierStyleLabel">•</span><p id="para0003" class="elsevierStylePara elsevierViewall">Dlat, Pdhb, and Pdhα1 levels were downregulated in acute myocardial infarction.</p></li><li class="elsevierStyleListItem" id="celistitem0003"><span class="elsevierStyleLabel">•</span><p id="para0004" class="elsevierStylePara elsevierViewall">Dlat, Pdhb, and Pdhα1 may be diagnostic markers in acute myocardial infarction.</p></li><li class="elsevierStyleListItem" id="celistitem0004"><span class="elsevierStyleLabel">•</span><p id="para0005" class="elsevierStylePara elsevierViewall">Pdhb showed the best diagnostic value for acute myocardial infarction.</p></li></ul></p></span>" ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1807593224000875?idApp=UINPBA00004N" "url" => "/18075932/000000790000000C/v123_202411010523/S1807593224000875/v123_202411010523/en/main.assets" ] "en" => array:19 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original articles</span>" "titulo" => "Effects of physical training on the metabolic profile of rats exposed to chronic restraint stress" "tieneTextoCompleto" => true "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "Carlos H.O. Reis, Sabriny G. Manzolli, Leonardo dos Santos, Ariana A. Silva, Ana Paula Lima-Leopoldo, André S. Leopoldo, Danilo S. Bocalini" "autores" => array:7 [ 0 => array:3 [ "nombre" => "Carlos H.O." "apellidos" => "Reis" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0001" ] ] ] 1 => array:3 [ "nombre" => "Sabriny G." "apellidos" => "Manzolli" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0001" ] ] ] 2 => array:4 [ "nombre" => "Leonardo" "apellidos" => "dos Santos" "email" => array:1 [ 0 => "leonardo.santos@ufes.br" ] "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0002" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">*</span>" "identificador" => "PC_cor2Xeth1Z2VU" ] ] ] 3 => array:3 [ "nombre" => "Ariana A." "apellidos" => "Silva" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0001" ] ] ] 4 => array:3 [ "nombre" => "Ana Paula" "apellidos" => "Lima-Leopoldo" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0001" ] ] ] 5 => array:3 [ "nombre" => "André S." "apellidos" => "Leopoldo" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0001" ] ] ] 6 => array:3 [ "nombre" => "Danilo S." "apellidos" => "Bocalini" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0001" ] ] ] ] "afiliaciones" => array:2 [ 0 => array:3 [ "entidad" => "Experimental Physiology and Biochemistry Laboratory, Physical Education and Sports Center of the Universidade Federal do Espirito Santo – Campus Goiabeiras, Vitória, ES, Brazil" "etiqueta" => "a" "identificador" => "aff0001" ] 1 => array:3 [ "entidad" => "Cardiac Electromechanics Laboratory, Postgraduation Program in Physiological Sciences, Health Sciences Center, Universidade Federal do Espirito Santo - Campus Maruipe, Vitoria, ES, Brazil" "etiqueta" => "b" "identificador" => "aff0002" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "PC_cor2Xeth1Z2VU" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:8 [ "identificador" => "fig0002" "etiqueta" => "Fig. 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 1010 "Ancho" => 3458 "Tamanyo" => 119414 ] ] "detalles" => array:1 [ 0 => array:3 [ "identificador" => "alt0002" "detalle" => "Fig " "rol" => "short" ] ] "descripcion" => array:1 [ "en" => "<p id="spara002" class="elsevierStyleSimplePara elsevierViewall">Values expressed as mean ± the SEM of corticosterone levels (Panel A), blood glucose curves after loading test (Panel B) and areas under the glycemic curves (Panel C) of groups C (control), T (trained), S (stressed), and TS (trained stressed) after 12 weeks of intervention (* p < 0.05 vs. C; <span class="elsevierStyleSup">†</span> p < 0.05 vs. T; <span class="elsevierStyleSup">‡</span> p < 0.05 vs. TS).</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0001" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0008">Introduction</span><p id="para0009" class="elsevierStylePara elsevierViewall">Stress is considered a state of homeostasis disruption characterized by specific and generalized adaptive responses to a perceived threat, challenge, or demand caused by psychological, environmental or physiological agents.<a class="elsevierStyleCrossRef" href="#bib0001"><span class="elsevierStyleSup">1</span></a> It can be categorized as either acute or chronic, both eliciting Sympathetic Nervous System (SNS) hyperactivity, catecholamine release, and dysregulation of the Hypothalamic-Pituitary-Adrenal (HPA) axis, resulting in elevated production of glucocorticoids ‒ cortisol in humans and corticosterone in rodents,<a class="elsevierStyleCrossRef" href="#bib0002"><span class="elsevierStyleSup">2</span></a> recognized as primary stress indicators.<a class="elsevierStyleCrossRef" href="#bib0003"><span class="elsevierStyleSup">3</span></a></p><p id="para0010" class="elsevierStylePara elsevierViewall">While short-term stress responses help individuals cope with immediate challenges, chronic or severe stress can adversely affect both physical and mental health, contributing to the development of pathological conditions, particularly cardiovascular diseases.<a class="elsevierStyleCrossRef" href="#bib0004"><span class="elsevierStyleSup">4</span></a> Additionally, the increased SNS and HPA activities are associated not only with increased heart rate and blood pressure, and suppression of the immune system but also with insulin resistance<a class="elsevierStyleCrossRef" href="#bib0007"><span class="elsevierStyleSup">7</span></a> and increased serum levels of glucose and lipids.<a class="elsevierStyleCrossRef" href="#bib0005"><span class="elsevierStyleSup">5</span></a></p><p id="para0011" class="elsevierStylePara elsevierViewall">Several strategies have been explored to combat stress-related complications.<a class="elsevierStyleCrossRef" href="#bib0008"><span class="elsevierStyleSup">8</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0009"><span class="elsevierStyleSup">9</span></a> Among these, physical exercise stands out as a clinically important tool in the control of stress symptoms.<a class="elsevierStyleCrossRef" href="#bib0010"><span class="elsevierStyleSup">10</span></a> Increased physical activity and reduced sedentary behavior are particularly crucial strategies in preventing, managing, and treating various stages of functional impairment caused by chronic stress.<a class="elsevierStyleCrossRef" href="#bib0008"><span class="elsevierStyleSup">8</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0011"><span class="elsevierStyleSup">11</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0012"><span class="elsevierStyleSup">12</span></a></p><p id="para0012" class="elsevierStylePara elsevierViewall">Actually, a Silva et al.<a class="elsevierStyleCrossRef" href="#bib0006"><span class="elsevierStyleSup">6</span></a> study conducted on rats exposed to chronic stress and physical training showed that adaptations induced by exercise attenuate stress-induced hypertension in rats but not the impairments on the myocardial mechanics. The significance of physical exercise is further evidenced by previous studies<a class="elsevierStyleCrossRef" href="#bib0008"><span class="elsevierStyleSup">8</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0011"><span class="elsevierStyleSup">11</span></a> that have demonstrated positive outcomes in clinical indicators of stress. Furthermore, physical exercise had been considered such effective strategy to reduce blood pressure,<a class="elsevierStyleCrossRef" href="#bib0013"><span class="elsevierStyleSup">13</span></a> improved blood glucose control,<a class="elsevierStyleCrossRef" href="#bib0014"><span class="elsevierStyleSup">14</span></a> increased insulin sensitivity,<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">15</span></a> enhanced immune response,<a class="elsevierStyleCrossRef" href="#bib0016"><span class="elsevierStyleSup">16</span></a> and improved functional capacity.<a class="elsevierStyleCrossRef" href="#bib0017"><span class="elsevierStyleSup">17</span></a></p><p id="para0013" class="elsevierStylePara elsevierViewall">However, if considering the research with basic approaches, the knowledge of physical exercise interventions by swimming to treat and in preventing metabolic outcomes associated with stress is still unclear. To understand the effects of stress in a more invasive way, protocols used in animal models are developed to advance the discovery of the mechanisms involved in stress, as well as the physiological and morphological repercussions<a class="elsevierStyleCrossRef" href="#bib0018"><span class="elsevierStyleSup">18</span></a> and understanding the interaction between various stimuli associated with physical activity.<a class="elsevierStyleCrossRef" href="#bib0019"><span class="elsevierStyleSup">19</span></a> Thus, this study aimed to evaluate the effect of physical training by swimming on the metabolic parameters of rats subjected to restraint stress.</p></span><span id="sec0002" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0009">Material and methods</span><span id="sec0003" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0010">Animals and experimental groups</span><p id="para0014" class="elsevierStylePara elsevierViewall">Twenty-nine male young Wistar rats (10‒12 weeks-old), weighing 200‒250g, were sourced from the Animal Facility at the Federal University of Espirito Santo for inclusion in this study. The animals were housed individually in cages, provided with “<span class="elsevierStyleItalic">ad libitum</span>” water and Nuvital CR-1 rat feed, in a controlled environment maintained at the temperature of approximately 22°C, humidity at 54%, and a 12-hour light/dark cycle. All protocols were in accordance with the recommendations of the National Council for Animal Experimentation. This study was previously approved by the Institutional Ethics Committee on Animal Use (number 21/2021).</p><p id="para0015" class="elsevierStylePara elsevierViewall">The animals were randomly divided into four experimental groups as follows: Control (C; n = 10), consisting of animals that remained at rest for twelve weeks; Trained (T; n = 10), with animals that underwent twelve weeks of swimming training; Stressed (S; n = 10), animals that remained at rest and submitted to restraint stress for twelve weeks; and Trained/Stressed (TS; n = 10), comprising animals that underwent twelve weeks of swimming training while simultaneously being subjected to restraint stress.</p></span><span id="sec0004" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0011">Physical exercise protocol</span><p id="para0016" class="elsevierStylePara elsevierViewall">The swimming exercise protocol follows the procedures previously established by this group.<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">20</span></a> The training program began with a 6-day adaptation phase, during which animals swam for 10 minutes on the first day, with each subsequent session adding ten minutes until completing 60 minutes per session. Then, a training phase was applied and maintained (60 minutes per session, five days a week) for 12 weeks. The pool water was constantly agitated using a pump to prevent the animals from diving or floating during the sessions, and a thermostat-controlled electric heating system maintained the water temperature between 32‒34°C, according to recommendations for rodent exercise regimes.<a class="elsevierStyleCrossRef" href="#bib0021"><span class="elsevierStyleSup">21</span></a></p></span><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0012">Chronic stress protocol</span><p id="para0017" class="elsevierStylePara elsevierViewall">Chronic stress was attained using immobilization individually in a Polyvinyl chloride (PVC) cylinder. Restraint stress is a widely utilized model of emotional stress in animal studies.<a class="elsevierStyleCrossRef" href="#bib0012"><span class="elsevierStyleSup">12</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0022"><span class="elsevierStyleSup">22</span></a> In the present study, animals were individually restrained in an opaque PVC cylinder (brown), measuring 20 cm in length and 6 cm in diameter, with closed ends and perforations for air circulation and maintenance of the ambient temperature at 25°C. This restraint lasted for 60 minutes per day, five days a week, over 12 weeks, according to established protocol.<a class="elsevierStyleCrossRef" href="#bib0012"><span class="elsevierStyleSup">12</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0022"><span class="elsevierStyleSup">22</span></a> This confinement severely restricts the animal movement, allowing only respiratory movements. In addition, the animal was kept away from contact with feces and with the tail raised, since these factors could intensify the stress stimulus beyond the intended level.</p></span><span id="sec0006" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0013">Physical fitness assessment</span><p id="para0018" class="elsevierStylePara elsevierViewall">The physical fitness and capacity were assessed in all experimental groups at the beginning and end of the protocols, using the maximal physical capacity test as previously established.<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">20</span></a> Briefly, animals were weighed, and loads equivalent to 10% of their body mass were affixed to their tails to assess physical capacity. The maximum swimming duration until the animal reached complete exhaustion was recorded, with exhaustion defined as submersion for at least 10 seconds.<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">20</span></a></p></span><span id="sec0007" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0014">Blood biomarkers associated with stress</span><p id="para0019" class="elsevierStylePara elsevierViewall">After undergoing a 15-hour fast, glucose, cholesterol and triglyceride concentrations were measured from blood samples collected from the caudal vein using the equipment Accuttrend (Roche Diagnostic Brazil Ltda., Brazil).</p><p id="para0020" class="elsevierStylePara elsevierViewall">Subsequently, rats underwent a glucose tolerance test. Baseline blood samples were obtained from the tail, followed by glucose administration (2 g/kg, i.p.), as protocols were previously established.<a class="elsevierStyleCrossRef" href="#bib0023"><span class="elsevierStyleSup">23</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0024"><span class="elsevierStyleSup">24</span></a> Blood samples were then collected at 30, 60, 120, 180 and 280 minutes post-glucose administration. Glucose levels were determined using the same equipment (Accuttrend, Roche Diagnostic Brazil Ltda., Brazil), and glucose tolerance was analyzed based on the area under the glycemic response curve.</p></span><span id="sec0008" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0015">Assessment of stress intensity</span><p id="para0021" class="elsevierStylePara elsevierViewall">After 48 hours of physical fitness assessment, all animals underwent a 12-hour fast and were anesthetized with pentobarbital (Fontoveter Animal Pharmaceuticals and Cosmetics Ltd, São Paulo, Brazil) at a dosage of 50 mg/kg and euthanized by decapitation. Blood samples were collected in heparinized tubes, centrifuged at 3000 <span class="elsevierStyleItalic">g</span> for 15 minutes at 4°C, and the serum was stored in an ultra-freezer at −80°C. Corticosterone levels were determined using a specific radioimmunoassay kit (Coat-A-Count Rat Corticosterone – Diagnostic Products Corporation, Los Angeles, USA), with a detection limit of approximately 16 nmoL/L.</p></span><span id="sec0009" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0016">Statistical analyses</span><p id="para0022" class="elsevierStylePara elsevierViewall">Data are presented as mean ± Standard Error of Mean (SEM). The D'Agostino-Pearson test was applied to analyze the normality distribution. For comparisons, the two-way ANOVA test was used, complemented with Bonferroni's or Dunn's post hoc tests, as necessary. All tests and graphics were performed using GraphPad Prism software (version 8.0, GraphPad Softwares Inc., San Diego, CA, USA). The significance level was set at 0.05.</p></span></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0017">Results</span><p id="para0023" class="elsevierStylePara elsevierViewall">As shown in <a class="elsevierStyleCrossRef" href="#fig0001">Fig. 1</a>, there was no significant difference (p > 0.05) in the physical capacity of the groups before the protocols. However, after 12 weeks of training, the groups T (before: 102 ± 23 vs. after: 273 ± 61 seconds) and TS (before: 97 ± 14 vs. after: 256 ± 50 seconds) improved their physical capacity, without distinction between them. Moreover, the physical capacity of the untrained groups remained unchanged after this period (group C, before: 86 ± 45 vs. after: 92 ± 46 seconds and group S, before: 96 ± 33 vs. after: 104 ± 32 seconds). As a result, the training protocol was able to improve 169 ± 21% and 162 ± 22% of the physical capacity of groups T and TS, respectively.</p><elsevierMultimedia ident="fig0001"></elsevierMultimedia><p id="para0024" class="elsevierStylePara elsevierViewall">The corticosterone levels (<a class="elsevierStyleCrossRef" href="#fig0002">Fig. 2A</a>), as measured in the experimental groups 12 weeks after protocols, was significantly increased in the stressed animals (335 ± 9 nmoL/L) them C (141 ± 3 nmoL/L), T (174 ± 3 nmoL/L) and TS (231 ± 7 nmoL/L). However, the strength training slightly elevated corticosterone levels in nonstressed groups, this exercise protocol was able to partially prevent the corticosterone increase induced by the chronic stress. which also differed from each other. No effects on the basal levels (fasting) of serum glucose (C: 100 ± 4, T: 97 ± 3, S: 100 ± 4, TS: 97 ± 5 mg/dL), cholesterol (C: 161 ± 3, T: 156 ± 4, S: 178 ± 8, TS: 258 ± 2 mg/dL) and triglyceride (C: 123 ± 6, T: 123 ± 6, S: 130 ± 8, TS: 127 ± 9 mg/dL).</p><elsevierMultimedia ident="fig0002"></elsevierMultimedia><p id="para0025" class="elsevierStylePara elsevierViewall">In terms of glycemic tolerance, as shown in <a class="elsevierStyleCrossRef" href="#fig0002">Fig. 2B</a>, the glucose levels in group S remained consistently higher (p < 0.05) following glucose administration compared to groups C, T, and TS, which showed no significant differences between them. When considering the overall blood glucose patterns throughout this protocol, the area under the curve values (<a class="elsevierStyleCrossRef" href="#fig0002">Fig. 2C</a>) for group S (913 ± 30 AU) was notably elevated compared to groups C (644 ± 10 AU), T (649 ± 9 AU), and TS (673 ± 12 AU), with no significant variances observed between the lattes three groups. Together, these findings clearly indicate impaired glycemic control in animals submitted to chronic stress protocol, which was significantly prevented by the strength-training program.</p></span><span id="sec0011" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0018">Discussion</span><p id="para0026" class="elsevierStylePara elsevierViewall">Physical inactivity is directly associated with various health risk factors, including stress,<a class="elsevierStyleCrossRef" href="#bib0026"><span class="elsevierStyleSup">26</span></a> which can diminish life expectancy within the population.<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">25</span></a> Conversely, regular physical exercise is recognized as an important protective measure against adverse effects of stress<a class="elsevierStyleCrossRef" href="#bib0006"><span class="elsevierStyleSup">6</span></a> and numerous stress-associated chronic diseases such as cardiovascular diseases, diabetes, obesity<a class="elsevierStyleCrossRef" href="#bib0027"><span class="elsevierStyleSup">27</span></a> and atherosclerosis.<a class="elsevierStyleCrossRef" href="#bib0028"><span class="elsevierStyleSup">28</span></a></p><p id="para0027" class="elsevierStylePara elsevierViewall">Considering the exercise protocol used in this study, this type of training has been widely employed to investigate the repercussions of physical exercise,<a class="elsevierStyleCrossRef" href="#bib0006"><span class="elsevierStyleSup">6</span></a> proving effective in enhancing physical capacity,<a class="elsevierStyleCrossRef" href="#bib0029"><span class="elsevierStyleSup">29</span></a> albeit potentially inducing acute stress-like effects.<a class="elsevierStyleCrossRef" href="#bib0006"><span class="elsevierStyleSup">6</span></a> In the present study, even the physical capacity of animals subjected to stress was significantly improved by the training protocol, aligning with findings from previous reports.<a class="elsevierStyleCrossRef" href="#bib0006"><span class="elsevierStyleSup">6</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">30</span></a> Additionally, serum corticosterone levels in animals exposed to chronic stress were not elevated when combined with physical exercise. Given that glucocorticoids are considered biological markers for chronic stress, elevated levels have been associated with an increased risk of developing conditions such as hypertension, atherosclerosis, and heart failure.<a class="elsevierStyleCrossRef" href="#bib0027"><span class="elsevierStyleSup">27</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0028"><span class="elsevierStyleSup">28</span></a></p><p id="para0028" class="elsevierStylePara elsevierViewall">No changes in glucose, cholesterol, and triglyceride levels were observed in this study, diverging from findings in other studies.<a class="elsevierStyleCrossRef" href="#bib0031"><span class="elsevierStyleSup">31</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0032"><span class="elsevierStyleSup">32</span></a> It is postulated that duration, intensity, and volume of stress exposure may have influenced this outcome. Prior research<a class="elsevierStyleCrossRef" href="#bib0006"><span class="elsevierStyleSup">6</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0033"><span class="elsevierStyleSup">33</span></a> indicated that different types of exercise confer benefits against stress-induced pathological changes. Thus, as demonstrated in the present study, physical exercise led to enhanced regulation of glycemic control.<a class="elsevierStyleCrossRef" href="#bib0034"><span class="elsevierStyleSup">34</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">35</span></a> Although the precise mechanism underlying this improvement remains unexplored, increased glucose uptake by the muscle due to the increased amount and activity of the GLUT4 are suggested to play an important role in this response.<a class="elsevierStyleCrossRef" href="#bib0036"><span class="elsevierStyleSup">36</span></a> Therefore, employing distinct pathways for GLUT4 translocation emerges as a principal mechanism for glucose uptake by skeletal muscle tissue. The energy discrepancy induced by muscle fiber contraction serves as the primary stimulus for AMP-Kinase (AMPKK), an enzyme that activates AMPK, subsequently facilitating the translocation of GLUT4 vesicles to the cell membrane.<a class="elsevierStyleCrossRef" href="#bib0036"><span class="elsevierStyleSup">36</span></a></p><p id="para0029" class="elsevierStylePara elsevierViewall">Furthermore, stress has been shown to compromise the insulin response.<a class="elsevierStyleCrossRef" href="#bib0037"><span class="elsevierStyleSup">37</span></a> Since insulin plays a critical role in regulating blood glucose homeostasis, the development of insulin resistance is one of the main factors contributing to the onset of type II diabetes, a condition already correlated with stress.<a class="elsevierStyleCrossRef" href="#bib0038"><span class="elsevierStyleSup">38</span></a> From this perspective, regular physical exercise has been recognized as an important strategy to improve insulin response.<a class="elsevierStyleCrossRef" href="#bib0032"><span class="elsevierStyleSup">32</span></a> Although our the authors did not evaluate the insulin response in the present study, the authors believe that the preserved glycemic behavior of stressed and trained animals to glucose loading may be justified by an improved insulin response by some mechanisms previously documented in the literature.<a class="elsevierStyleCrossRef" href="#bib0014"><span class="elsevierStyleSup">14</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">35</span></a> Nonetheless, further studies are needed to confirm this hypothesis.</p><p id="para0030" class="elsevierStylePara elsevierViewall">As evidenced in this study, physical exercise led to a reduction in corticosterone levels<a class="elsevierStyleCrossRef" href="#bib0002"><span class="elsevierStyleSup">2</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0039"><span class="elsevierStyleSup">39</span></a> and improved regulation of glycemic control.<a class="elsevierStyleCrossRef" href="#bib0034"><span class="elsevierStyleSup">34</span></a> The decrease in corticosterone levels has been linked to a reduction in stress intensity, as observed in previous research;<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">40</span></a><span class="elsevierStyleSup">,</span><span class="elsevierStyleSup">41</span> however, further investigations are warranted to fully understand this response. Elevated circulating glucocorticoid levels diminish the sensitivity of hepatic and peripheral tissues to insulin, resulting in increased hepatic glucose production and impaired glucose absorption by muscles and adipose tissue.<a class="elsevierStyleCrossRef" href="#bib0038"><span class="elsevierStyleSup">38</span></a> In line with the authors's findings, plasma corticosterone levels were found to rise during and up to 1 hour after an episode of acute stress, coinciding with a return to baseline glycemic levels.<a class="elsevierStyleCrossRef" href="#bib0038"><span class="elsevierStyleSup">38</span></a> As demonstrated in the present study, physical exercise attenuated corticosterone levels<a class="elsevierStyleCrossRef" href="#bib0002"><span class="elsevierStyleSup">2</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0039"><span class="elsevierStyleSup">39</span></a> and improved regulation of glycemic control<a class="elsevierStyleCrossRef" href="#bib0034"><span class="elsevierStyleSup">34</span></a> of the stressed rat. Actually, the decrease in corticosterone levels has been linked to a reduction in stress intensity, as observed in other studies;<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">40</span></a><span class="elsevierStyleSup">,</span><span class="elsevierStyleSup">41</span> however, more investigations are warranted to clarify this response. Elevated circulating glucocorticoid levels diminish the sensitivity of hepatic and peripheral tissues to insulin, leading to an increase in hepatic glucose production and impaired absorption by muscles and adipose tissue.<a class="elsevierStyleCrossRef" href="#bib0038"><span class="elsevierStyleSup">38</span></a> In line with the present findings, plasma corticosterone levels were found to increase during and up to 1 hour after an episode of acute stress, coinciding with a return to baseline glycemic levels.<a class="elsevierStyleCrossRef" href="#bib0038"><span class="elsevierStyleSup">38</span></a></p><p id="para0031" class="elsevierStylePara elsevierViewall">In general, the results of this study suggest that exercise effectively attenuated the stress effects on glucose control and corticosterone levels, thus serving as a protective strategy that influences the hypothalamic-pituitary-adrenal axis, and the secretion of Adrenocorticotropic Hormone (ACTH). This reduction in serum corticosterone levels aligns with similar results found in previous studies.<a class="elsevierStyleCrossRef" href="#bib0006"><span class="elsevierStyleSup">6</span></a><span class="elsevierStyleSup">,</span><a class="elsevierStyleCrossRef" href="#bib0039"><span class="elsevierStyleSup">39</span></a> For instance, by Droste et al.<span class="elsevierStyleSup">42</span> observed substantial changes in HPA axis regulation and body composition among exercised mice. Notably, their findings included the loss of adrenal asymmetry due to adrenocortical enlargement and sympathoadrenomedullary overstimulation, indicative of potential changes in the sympathoadrenomedullary system and hormonal secretion patterns in the HPA axis. The increased circulating corticosterone levels found in exercised animals compared to controls may be considered as anticipatory and adaptive responses aimed at supporting metabolism in the face of the demand of physical activity, without concomitant increases in ACTH release by the HPA axis.<span class="elsevierStyleSup">43</span> Therefore, sympathetic activity in the adrenal medulla serves as a positive modulator of adrenocortical sensitivity to ACTH. Thus, physical exercise appears to attenuate HPA axis activity in response to other stressors, providing an adaptive state with reduced ACTH stimulation,<span class="elsevierStyleSup">43</span> which may explain the observed reduction in plasma corticosterone levels.</p></span><span id="sec0012" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0019">Conclusion</span><p id="para0032" class="elsevierStylePara elsevierViewall">Animals subjected to restraint stress, even in the absence of basal glycemic alterations, exhibited impaired glycemic control, accompanied by glucose intolerance. However, this response was mitigated by physical training. Given that physical exercise is advocated by the World Health Organization and other health organizations for maintaining health, additional research is warranted to uncover its additional benefits in mitigating the adverse effects of chronic stress. Animals submitted to restraint stress, even in the absence of basal glycemic alterations, exhibited impaired glycemic control characterized by glucose intolerance. However, this alteration was mitigated by physical training. Given that physical exercise is advocated by the World Health Organization and other health organizations for maintaining health, additional research is warranted to uncover its additional benefits in mitigating the adverse effects of chronic stress.</p></span><span id="sec0013" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0020">Funding</span><p id="para0033" class="elsevierStylePara elsevierViewall">This research received no specific grant from any funding agency in the public, commercial, or not-for-profit sectors.</p></span><span id="sec0013a" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0020a">CRediT authorship contribution statement</span><p id="para0033a" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleBold">Carlos H.O. Reis:</span> Visualization, Investigation, Writing – original draft. <span class="elsevierStyleBold">Sabriny G. Manzolli:</span> Investigation. <span class="elsevierStyleBold">Leonardo dos Santos:</span> . <span class="elsevierStyleBold">Ariana A. Silva:</span> . <span class="elsevierStyleBold">Ana Paula Lima-Leopoldo:</span> Visualization, Investigation, Writing – review & editing. <span class="elsevierStyleBold">André S. Leopoldo:</span> Visualization, Investigation, Writing – review & editing. <span class="elsevierStyleBold">Danilo S. Bocalini:</span> Visualization, Investigation, Writing – original draft, Writing – review & editing.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:12 [ 0 => array:3 [ "identificador" => "xres2291017" "titulo" => "Highlights" "secciones" => array:1 [ 0 => array:1 [ "identificador" => "abss0001" ] ] ] 1 => array:3 [ "identificador" => "xres2291016" "titulo" => "Abstract" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abss0002" "titulo" => "Introduction" ] 1 => array:2 [ "identificador" => "abss0003" "titulo" => "Methods" ] 2 => array:2 [ "identificador" => "abss0004" "titulo" => "Results" ] 3 => array:2 [ "identificador" => "abss0005" "titulo" => "Conclusion" ] ] ] 2 => array:2 [ "identificador" => "xpalclavsec1903364" "titulo" => "Keyword" ] 3 => array:2 [ "identificador" => "sec0001" "titulo" => "Introduction" ] 4 => array:3 [ "identificador" => "sec0002" "titulo" => "Material and methods" "secciones" => array:7 [ 0 => array:2 [ "identificador" => "sec0003" "titulo" => "Animals and experimental groups" ] 1 => array:2 [ "identificador" => "sec0004" "titulo" => "Physical exercise protocol" ] 2 => array:2 [ "identificador" => "sec0005" "titulo" => "Chronic stress protocol" ] 3 => array:2 [ "identificador" => "sec0006" "titulo" => "Physical fitness assessment" ] 4 => array:2 [ "identificador" => "sec0007" "titulo" => "Blood biomarkers associated with stress" ] 5 => array:2 [ "identificador" => "sec0008" "titulo" => "Assessment of stress intensity" ] 6 => array:2 [ "identificador" => "sec0009" "titulo" => "Statistical analyses" ] ] ] 5 => array:2 [ "identificador" => "sec0010" "titulo" => "Results" ] 6 => array:2 [ "identificador" => "sec0011" "titulo" => "Discussion" ] 7 => array:2 [ "identificador" => "sec0012" "titulo" => "Conclusion" ] 8 => array:2 [ "identificador" => "sec0013" "titulo" => "Funding" ] 9 => array:2 [ "identificador" => "sec0013a" "titulo" => "CRediT authorship contribution statement" ] 10 => array:2 [ "identificador" => "xack786276" "titulo" => "Acknowledgments" ] 11 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2023-10-27" "fechaAceptado" => "2024-05-28" "PalabrasClave" => array:1 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keyword" "identificador" => "xpalclavsec1903364" "palabras" => array:3 [ 0 => "Stress" 1 => "Glucose Tolerance" 2 => "Physical exercise and fitness" ] ] ] ] "tieneResumen" => true "highlights" => array:2 [ "titulo" => "Highlights" "resumen" => "<span id="abss0001" class="elsevierStyleSection elsevierViewall"><p id="spara003" class="elsevierStyleSimplePara elsevierViewall"><ul class="elsevierStyleList" id="celist0001"><li class="elsevierStyleListItem" id="celistitem0001"><span class="elsevierStyleLabel">•</span><p id="para0001" class="elsevierStylePara elsevierViewall">Swimming training significantly prevented stress-induced corticosterone increase in rats.</p></li><li class="elsevierStyleListItem" id="celistitem0002"><span class="elsevierStyleLabel">•</span><p id="para0002" class="elsevierStylePara elsevierViewall">Serum glucose, cholesterol and triglyceride were unchanged by chronic stress or exercise.</p></li><li class="elsevierStyleListItem" id="celistitem0003"><span class="elsevierStyleLabel">•</span><p id="para0003" class="elsevierStylePara elsevierViewall">Chronic stress protocol induced glucose intolerance in the rats.</p></li><li class="elsevierStyleListItem" id="celistitem0004"><span class="elsevierStyleLabel">•</span><p id="para0004" class="elsevierStylePara elsevierViewall">The training preserved the normal glycemic response after glucose loading in stressed rats.</p></li></ul></p></span>" ] "resumen" => array:1 [ "en" => array:3 [ "titulo" => "Abstract" "resumen" => "<span id="abss0002" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0003">Introduction</span><p id="spara004" class="elsevierStyleSimplePara elsevierViewall">Despite strong evidences supporting the protective role of exercise against stress-induced repercussions, the literature remains inconclusive regarding metabolic aspects. Therefore, this study aimed to evaluate the effect of Physical Training (PT) by swimming on the metabolic parameters of rats subjected to restraint stress.</p></span> <span id="abss0003" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0004">Methods</span><p id="spara005" class="elsevierStyleSimplePara elsevierViewall">Wistar rats (n = 40) were divided into four groups: Control (C), Trained (T), Stressed (S), and Trained/Stressed (TS). The restraint stress protocol involved confining the animals in PVC pipes for 60 minutes/day for 12 weeks. Concurrently, the swimming PT protocol was performed without additional load in entailed sessions of 60 minutes conducted five days a week for the same duration. The following parameters were analyzed: fitness progression assessed by the physical capacity test, body mass, serum level of glucose, triglyceride, cholesterol and corticosterone, as well as glycemic tolerance test, evaluated after glucose administration (2 g/kg, i.p.).</p></span> <span id="abss0004" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0005">Results</span><p id="spara006" class="elsevierStyleSimplePara elsevierViewall">Trained groups (T and TS) exhibited enhanced physical capacity (169 ± 21 and 162 ± 22% increase, respectively) compared to untrained groups (C: 9 ± 5 and S: 11 ± 13% increase). Corticosterone levels were significantly higher in the S group (335 ± 9 nmoL/L) compared to C (141 ± 3 nmoL/L), T (174 ± 3 nmoL/L) and TS (231 ± 7 nmoL/L), which did not differ from each other. There were no significant changes in serum glucose, cholesterol, and triglyceride levels among the groups. However, the glycemic curve after glucose loading revealed increased glycemia in the S group (area under curve 913 ± 30 AU) but the TS group exhibited values (673 ± 12 AU) similar to the groups C (644 ± 10 AU) and T (649 ± 9 AU).</p></span> <span id="abss0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cesectitle0006">Conclusion</span><p id="spara007" class="elsevierStyleSimplePara elsevierViewall">Swimming-based training attenuated stress-induced corticosterone release and prevented glucose intolerance in rats, reinforcing the importance of exercise as a potential strategy to mitigate the pathophysiological effects of stress.</p></span>" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abss0002" "titulo" => "Introduction" ] 1 => array:2 [ "identificador" => "abss0003" "titulo" => "Methods" ] 2 => array:2 [ "identificador" => "abss0004" "titulo" => "Results" ] 3 => array:2 [ "identificador" => "abss0005" "titulo" => "Conclusion" ] ] ] ] "multimedia" => array:2 [ 0 => array:8 [ "identificador" => "fig0001" "etiqueta" => "Fig. 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 815 "Ancho" => 1750 "Tamanyo" => 57155 ] ] "detalles" => array:1 [ 0 => array:3 [ "identificador" => "alt0001" "detalle" => "Fig " "rol" => "short" ] ] "descripcion" => array:1 [ "en" => "<p id="spara001" class="elsevierStyleSimplePara elsevierViewall">Values expressed as mean ± SEM of the physical capacity of animals in experimental groups before and after 12 weeks of intervention (* p < 0.05 vs. before; <span class="elsevierStyleSup">†</span> p < 0.05 vs. C; <span class="elsevierStyleSup">‡</span> p < 0.05 vs. E).</p>" ] ] 1 => array:8 [ "identificador" => "fig0002" "etiqueta" => "Fig. 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 1010 "Ancho" => 3458 "Tamanyo" => 119414 ] ] "detalles" => array:1 [ 0 => array:3 [ "identificador" => "alt0002" "detalle" => "Fig " "rol" => "short" ] ] "descripcion" => array:1 [ "en" => "<p id="spara002" class="elsevierStyleSimplePara elsevierViewall">Values expressed as mean ± the SEM of corticosterone levels (Panel A), blood glucose curves after loading test (Panel B) and areas under the glycemic curves (Panel C) of groups C (control), T (trained), S (stressed), and TS (trained stressed) after 12 weeks of intervention (* p < 0.05 vs. C; <span class="elsevierStyleSup">†</span> p < 0.05 vs. T; <span class="elsevierStyleSup">‡</span> p < 0.05 vs. TS).</p>" ] ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "cebibsec1" "bibliografiaReferencia" => array:40 [ 0 => array:3 [ "identificador" => "bib0001" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The concepts of stress and stress system disorders. Overview of physical and behavioral homeostasis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "GP Chrousos" 1 => "PW. Gold" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:7 [ "tituloSerie" => "JAMA" "fecha" => "1992" "volumen" => "267" "numero" => "9" "paginaInicial" => "1244" "paginaFinal" => "1252" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/1538563" "web" => "Medline" ] ] ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib0002" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Physical activity, exercise, depression and anxiety disorders" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "A. Ströhle" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1007/s00702-008-0092-x" "Revista" => array:7 [ "tituloSerie" => "J Neural Transm" "fecha" => "2009" "volumen" => "116" "numero" => "6" "paginaInicial" => "777" "paginaFinal" => "784" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18726137" "web" => "Medline" ] ] ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bib0003" "etiqueta" => "3" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Different stress modalities result in distinct steroid hormone responses by male rats" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "ML Andersen" 1 => "M Bignotto" 2 => "RB Machado" 3 => "S. Tufik" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1590/s0100-879x2004000600003" "Revista" => array:7 [ "tituloSerie" => "Braz J Med Biol Res" "fecha" => "2004" "volumen" => "37" "numero" => "6" "paginaInicial" => "791" "paginaFinal" => "797" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15264021" "web" => "Medline" ] ] ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bib0004" "etiqueta" => "4" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Effects of stress on the development and progression of cardiovascular disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "M Kivimäki" 1 => "A. Steptoe" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1038/nrcardio.2017.189" "Revista" => array:7 [ "tituloSerie" => "Nat Rev Cardiol" "fecha" => "2018" "volumen" => "15" "numero" => "4" "paginaInicial" => "215" "paginaFinal" => "229" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/29213140" "web" => "Medline" ] ] ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bib0005" "etiqueta" => "5" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Peripheral and central effects of circulating catecholamines" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "AW Tank" 1 => "WD. Lee" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1002/cphy.c140007" "Revista" => array:7 [ "tituloSerie" => "Compr Physiol" "fecha" => "2015" "volumen" => "5" "numero" => "1" "paginaInicial" => "1" "paginaFinal" => "15" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/25589262" "web" => "Medline" ] ] ] ] ] ] ] ] 5 => array:3 [ "identificador" => "bib0006" "etiqueta" => "6" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Physical exercise attenuates stress-induced hypertension in rats but not the impairments on the myocardial mechanics" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "AA Silva" 1 => "MS Perilhão" 2 => "LA Portes" 3 => "AJ Serra" 4 => "PJF Tucci" 5 => "AS Leopoldo" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1097/HJH.0000000000003044" "Revista" => array:7 [ "tituloSerie" => "J Hypertens" "fecha" => "2022" "volumen" => "40" "numero" => "3" "paginaInicial" => "528" "paginaFinal" => "535" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/34772858" "web" => "Medline" ] ] ] ] ] ] ] ] 6 => array:3 [ "identificador" => "bib0007" "etiqueta" => "7" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Stress-induced cardiometabolic perturbations, increased oxidative stress and ACE/ACE2 imbalance are improved by endurance training in rats" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "V Guzzoni" 1 => "A Sanches" 2 => "R Costa" 3 => "LB Souza" 4 => "LT Firoozmand" 5 => "ICME Abreu" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:3 [ "tituloSerie" => "Life Sci" "fecha" => "2022" "volumen" => "305" ] ] ] ] ] ] 7 => array:3 [ "identificador" => "bib0008" "etiqueta" => "8" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Nível de estresse em adultos após 12 meses de prática regular de atividade física" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "M Nunomura" 1 => "LAC Teixeira" 2 => "MRF. Caruso" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Rev Mackenzie Educação Física Esporte" "fecha" => "2004" "volumen" => "3" "numero" => "3" "paginaInicial" => "125" "paginaFinal" => "134" ] ] ] ] ] ] 8 => array:3 [ "identificador" => "bib0009" "etiqueta" => "9" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Oxidative stress and aging: role of physical exercise" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "C Moraes" 1 => "RC. Sampaio" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Motriz" "fecha" => "2010" "volumen" => "16" "numero" => "2" "paginaInicial" => "506" "paginaFinal" => "515" ] ] ] ] ] ] 9 => array:3 [ "identificador" => "bib0010" "etiqueta" => "10" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Exercise training in healthy Type A middle-aged men: effects on behavioral and cardiovascular responses" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "JA Blumenthal" 1 => "CF Emery" 2 => "MA Walsh" 3 => "DR Cox" 4 => "CM Kuhn" 5 => "RB Williams" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Psychosomatic Med" "fecha" => "1988" "volumen" => "50" "paginaInicial" => "418" "paginaFinal" => "433" ] ] ] ] ] ] 10 => array:3 [ "identificador" => "bib0011" "etiqueta" => "11" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Controle do estresse e hipertensão arterial sistêmica" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "MNL. Lipp" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Rev Bras Hipertens" "fecha" => "2007" "volumen" => "14" "numero" => "2" "paginaInicial" => "89" "paginaFinal" => "93" ] ] ] ] ] ] 11 => array:3 [ "identificador" => "bib0012" "etiqueta" => "12" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Chronic stress improves the myocardial function without altering L-type Ca+2 channel activity in rats" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "TB Nascimento" 1 => "DHS Campos" 2 => "AS Leopoldo" 3 => "AP Lima-Leopoldo" 4 => "K Okoshi" 5 => "S Cordellini" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Arq Bras Cardiol" "fecha" => "2012" "volumen" => "99" "numero" => "4" "paginaInicial" => "907" "paginaFinal" => "914" ] ] ] ] ] ] 12 => array:3 [ "identificador" => "bib0013" "etiqueta" => "13" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Swimming training lowers the resting blood pressure in individuals with hypertension" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "H Tanaka" 1 => "DR Basset Jr" 2 => "ET Howley" 3 => "DL Thompson" 4 => "M Ashraf" 5 => "FL Rawson" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1097/00004872-199715060-00012" "Revista" => array:6 [ "tituloSerie" => "J Hypertens" "fecha" => "1997" "volumen" => "15" "paginaInicial" => "651" "paginaFinal" => "657" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9218185" "web" => "Medline" ] ] ] ] ] ] ] ] 13 => array:3 [ "identificador" => "bib0014" "etiqueta" => "14" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Effect of frequency of physical exercise on glycemic control and body composition in type 2 diabetic patients" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "DMM Vancea" 1 => "JN Vancea" 2 => "MIF Pires" 3 => "AM Reis" 4 => "RB Moura" 5 => "SA. Dib" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1590/s0066-782x2009000100005" "Revista" => array:7 [ "tituloSerie" => "Arq Bras Cardiol" "fecha" => "2009" "volumen" => "92" "numero" => "1" "paginaInicial" => "23" "paginaFinal" => "30" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19219261" "web" => "Medline" ] ] ] ] ] ] ] ] 14 => array:3 [ "identificador" => "bib0015" "etiqueta" => "15" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Efeito do exercício físico na sensibilidade à insulina em mulheres obesas submetidas a programa de perda de peso: um ensaio clínico" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "MFF Mediano" 1 => "JSO Barbosa" 2 => "R Sichieri" 3 => "RA. Pereira" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Arq Bras Endocrinol Metab" "fecha" => "2007" "volumen" => "51" "numero" => "6" "paginaInicial" => "993" "paginaFinal" => "999" ] ] ] ] ] ] 15 => array:3 [ "identificador" => "bib0016" "etiqueta" => "16" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Exercício físico e sistema imunológico: mecanismos e integrações" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "CG Leandro" 1 => "E Nascimento" 2 => "R Manhães-de-Castro" 3 => "JA Duarte" 4 => "CMMB. de Castro" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Rev Port Ciênc Desp" "fecha" => "2002" "volumen" => "2" "numero" => "5" "paginaInicial" => "80" "paginaFinal" => "90" ] ] ] ] ] ] 16 => array:3 [ "identificador" => "bib0017" "etiqueta" => "17" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The role of exercise training in heart failure" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "J Downing" 1 => "GJ. Balady" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "J Am Coll Cardiol" "fecha" => "2011" "volumen" => "58" "numero" => "6" "paginaInicial" => "561" "paginaFinal" => "569" ] ] ] ] ] ] 17 => array:3 [ "identificador" => "bib0018" "etiqueta" => "18" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Efeito do estresse crônico de contenção sobre o reparo de cavidades ósseas: estudo histológico em tíbia de ratos" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "MCR Alves-Rezende" 1 => "R Kusuda" 2 => "LMN Alves" 3 => "RC Felipini" 4 => "R Okamoto" 5 => "T Okamoto" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Rev Odontol Araçatuba" "fecha" => "2009" "volumen" => "30" "numero" => "2" "paginaInicial" => "71" "paginaFinal" => "76" ] ] ] ] ] ] 18 => array:3 [ "identificador" => "bib0019" "etiqueta" => "19" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Chronic stress improves NO<span class="elsevierStyleInf">-</span> and Ca<span class="elsevierStyleInf">2+</span> flux-dependent vascular function: a pharmacological study" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "T Burder-Nascimento" 1 => "DHS Campos" 2 => "AC Cicogna" 3 => "S. Cordellini" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.5935/abc.20140207" "Revista" => array:7 [ "tituloSerie" => "Arq Bras Cardiol" "fecha" => "2015" "volumen" => "104" "numero" => "3" "paginaInicial" => "226" "paginaFinal" => "233" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/25884770" "web" => "Medline" ] ] ] ] ] ] ] ] 19 => array:3 [ "identificador" => "bib0020" "etiqueta" => "20" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Exercise training-induced enhancement in myocardial mechanics is lost after 2 weeks of detraining in rats" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "DS Bocalini" 1 => "EV Carvalho" 2 => "AF de Souza" 3 => "RF Levy" 4 => "PJ. Tucci" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1007/s00421-010-1406-x" "Revista" => array:7 [ "tituloSerie" => "Eur J Appl Physiol" "fecha" => "2010" "volumen" => "109" "numero" => "5" "paginaInicial" => "909" "paginaFinal" => "914" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20300769" "web" => "Medline" ] ] ] ] ] ] ] ] 20 => array:3 [ "identificador" => "bib0021" "etiqueta" => "21" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Resource book for the design of animal exercise protocols" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "KC Kregel" 1 => "DL Allen" 2 => "FW Booth" 3 => "MR Fleshner" 4 => "EJ Henriksen" 5 => "TI Much" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "edicion" => "1rd ed." "fecha" => "2006" "editorial" => "American Physiological Society" ] ] ] ] ] ] 21 => array:3 [ "identificador" => "bib0022" "etiqueta" => "22" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Efeitos adaptativos induzidos pelo estresse crônico imprevisível nos receptores do fator liberador de corticotrofina tipo 2 e de glicocorticóides no sistema nervoso central de ratos [tese]" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "MB. Malta" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:3 [ "fecha" => "2012" "editorial" => "Universidade de São Paulo" "editorialLocalizacion" => "São Paulo" ] ] ] ] ] ] 22 => array:3 [ "identificador" => "bib0023" "etiqueta" => "23" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Impact of stress reduction on negative school behavior in adolescents" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => "VA Barnes" 1 => "LB Bauza" 2 => "FA. Treiber" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Health Qual Life Outcomes" "fecha" => "2003" "volumen" => "23" "numero" => "1" "paginaInicial" => "10" ] ] ] ] ] ] 23 => array:3 [ "identificador" => "bib0024" "etiqueta" => "24" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Diffential vascular dysfunction in response to diets of differing macronutrient composition: a phenomenonological study" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "S Fatani" 1 => "LC Pickavance" 2 => "CJ Sadler" 3 => "JA Harrold" 4 => "R Cassidy" 5 => "JPH Wilding" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1186/1743-7075-4-1" "Revista" => array:6 [ "tituloSerie" => "Nutr Metab (Lond)" "fecha" => "2007" "volumen" => "4" "paginaInicial" => "1" "paginaFinal" => "6" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17204161" "web" => "Medline" ] ] ] ] ] ] ] ] 24 => array:3 [ "identificador" => "bib0025" "etiqueta" => "25" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Health predictors and conditions associated to moderate and vigorous physical activity among adults and elderly from Southern Brazil" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "SC Dumith" 1 => "FV Maciel" 2 => "JL Borchardt" 3 => "VS Alam" 4 => "FC Silveira" 5 => "RG Paulitsch" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:3 [ "tituloSerie" => "Rev Bras Epidemiol" "fecha" => "2019" "volumen" => "22" ] ] ] ] ] ] 25 => array:3 [ "identificador" => "bib0026" "etiqueta" => "26" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Role of physical activity and sedentary behavior in the mental health of preschoolers, children and adolescents: a systematic review and meta-analysis" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "M Rodriguez-Ayllon" 1 => "C Cadenas-Sánchez" 2 => "F Estévez-López" 3 => "EN Muñoz" 4 => "J Mora-Gonzalex" 5 => "JH Migueles" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1007/s40279-019-01099-5" "Revista" => array:7 [ "tituloSerie" => "Sports Med" "fecha" => "2019" "volumen" => "49" "numero" => "9" "paginaInicial" => "1383" "paginaFinal" => "1410" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/30993594" "web" => "Medline" ] ] ] ] ] ] ] ] 26 => array:3 [ "identificador" => "bib0027" "etiqueta" => "27" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Exercise as medicine - evidence for prescribing exercise as therapy in 26 different chronic diseases" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => "BK Pedersen" 1 => "B. Saltin" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Scand J Med Sci Sport" "fecha" => "2015" "volumen" => "25" "numero" => "Suppl 3" "paginaInicial" => "1" "paginaFinal" => "72" ] ] ] ] ] ] 27 => array:3 [ "identificador" => "bib0028" "etiqueta" => "28" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Exercise training mitigates ER stress and UCP2 deficiency-associated coronary vascular dysfunction in atherosclerosis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "J Hong" 1 => "E Park" 2 => "J Lee" 3 => "Y Lee" 4 => "BV Rooney" 5 => "Y. Park" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Sci Rep" "fecha" => "2021" "volumen" => "11" "numero" => "1" "paginaInicial" => "15449" ] ] ] ] ] ] 28 => array:3 [ "identificador" => "bib0029" "etiqueta" => "29" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Exercise downregulates HIPK2 and HIPK2 inhibition protects against myocardial infarction" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "Q Zhou" 1 => "J Deng" 2 => "J Yao" 3 => "J Song" 4 => "D Meng" 5 => "Y Zhu" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:3 [ "tituloSerie" => "EBioMedicine" "fecha" => "2021" "volumen" => "74" ] ] ] ] ] ] 29 => array:3 [ "identificador" => "bib0030" "etiqueta" => "30" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The impact of nine weeks swimming exercise on heart function in hypertensive and normotensive rats: role of cardiac oxidative stress" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "V Prokic" 1 => "S Plecevic" 2 => "J Bradic" 3 => "A Petkovic" 4 => "I Srejovic" 5 => "S Bolevich" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.23736/S0022-4707.19.09798-6" "Revista" => array:7 [ "tituloSerie" => "J Sports Med Phys Fitness" "fecha" => "2019" "volumen" => "59" "numero" => "12" "paginaInicial" => "2075" "paginaFinal" => "2083" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/31240902" "web" => "Medline" ] ] ] ] ] ] ] ] 30 => array:3 [ "identificador" => "bib0031" "etiqueta" => "31" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Vascular non-endothelial nitric oxide induced by swimming exercise stress in rats" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "AB Chies" 1 => "FM Corrêa" 2 => "CR de Andrade" 3 => "AA Rosa-e-Silva" 4 => "FC Pereira" 5 => "AM. de Oliveira" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1111/j.1440-1681.2003.03935.x" "Revista" => array:7 [ "tituloSerie" => "Clin Exp Pharmacol Physiol" "fecha" => "2003" "volumen" => "30" "numero" => "12" "paginaInicial" => "951" "paginaFinal" => "957" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/14678235" "web" => "Medline" ] ] ] ] ] ] ] ] 31 => array:3 [ "identificador" => "bib0032" "etiqueta" => "32" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Performance during a strenuous swimming session is associated with high blood lactate: pyruvate ratio and hypoglycemia in fasted rats" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "PB Travassos" 1 => "G Godoy" 2 => "HM De Souza" 3 => "R Curi" 4 => "RB. Bazotte" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Braz J Med Biol Res" "fecha" => "2018" "volumen" => "51" "numero" => "5" "paginaInicial" => "e7057" ] ] ] ] ] ] 32 => array:3 [ "identificador" => "bib0033" "etiqueta" => "33" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Wanderley-Teixeira V. Effect of swimming exercise, insulin-associated or not, on inflammatory cytokines, apoptosis, and collagen in diabetic rat placentas" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "MMS Pereira" 1 => "IMF de Melo" 2 => "VAÁ Braga" 3 => "ÁAC Teixeira" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1007/s00418-021-02069-7" "Revista" => array:7 [ "tituloSerie" => "Histochem Cell Biol" "fecha" => "2022" "volumen" => "157" "numero" => "4" "paginaInicial" => "467" "paginaFinal" => "479" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/35022821" "web" => "Medline" ] ] ] ] ] ] ] ] 33 => array:3 [ "identificador" => "bib0034" "etiqueta" => "34" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Effect of combination of chlorella intake and aerobic exercise training on glycemic control in type 2 diabetic rats" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "N Horii" 1 => "N Hasegawa" 2 => "S Fujie" 3 => "M Uchida" 4 => "K Iemitsu" 5 => "K Inoue" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.nut.2019.01.008" "Revista" => array:6 [ "tituloSerie" => "Nutrition" "fecha" => "2019" "volumen" => "63-64" "paginaInicial" => "45" "paginaFinal" => "50" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/30928787" "web" => "Medline" ] ] ] ] ] ] ] ] 34 => array:3 [ "identificador" => "bib0035" "etiqueta" => "35" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Glicocorticóides e síndrome metabólica: aspectos favoráveis do exercício físico nesta patofisiologia" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => "JR Pauli" 1 => "L Souza" 2 => "G Rogatto" 3 => "R Gomes" 4 => "E. Luciano" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Rev Port Cien Desp" "fecha" => "2007" "volumen" => "6" "numero" => "2" "paginaInicial" => "17" "paginaFinal" => "228" ] ] ] ] ] ] 35 => array:3 [ "identificador" => "bib0036" "etiqueta" => "36" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Effect of forced exercise and exercise withdrawal on memory, serum and hippocampal corticosterone levels in rats" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "M Radahmadi" 1 => "H Alaei" 2 => "MR Sharifi" 3 => "N. Hosseini" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1007/s00221-015-4349-y" "Revista" => array:7 [ "tituloSerie" => "Exp Brain Res" "fecha" => "2015" "volumen" => "233" "numero" => "10" "paginaInicial" => "2789" "paginaFinal" => "2799" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26070900" "web" => "Medline" ] ] ] ] ] ] ] ] 36 => array:3 [ "identificador" => "bib0037" "etiqueta" => "37" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Early life stress increases testosterone and corticosterone and alters stress physiology in zebra finches" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "JB Zito" 1 => "A Hanna" 2 => "N Kadoo" 3 => "ML. Tomaszycki" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1016/j.yhbeh.2017.08.001" "Revista" => array:6 [ "tituloSerie" => "Horm Behav" "fecha" => "2017" "volumen" => "95" "paginaInicial" => "57" "paginaFinal" => "64" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/28782547" "web" => "Medline" ] ] ] ] ] ] ] ] 37 => array:3 [ "identificador" => "bib0038" "etiqueta" => "38" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Stress coping and evolution of aerobic exercise performance: corticosterone levels in voles from a selection experiment" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "MM Lipowska" 1 => "ET Sadowska" 2 => "U Bauchinger" 3 => "P. Koteja" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:4 [ "tituloSerie" => "J Exp Biol" "fecha" => "2019" "volumen" => "222" "numero" => "Pt 20" ] ] ] ] ] ] 38 => array:3 [ "identificador" => "bib0039" "etiqueta" => "39" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Effects of long-term voluntary exercise on the mouse hypothalamic-pituitary-adrenocortical axis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "SK Droste" 1 => "A Gesing" 2 => "S Ulbricht" 3 => "MB Muller" 4 => "ACE Linthorst" 5 => "JMHM. Reul" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1210/en.2003-0097" "Revista" => array:7 [ "tituloSerie" => "Endocrinology" "fecha" => "2003" "volumen" => "144" "numero" => "7" "paginaInicial" => "3012" "paginaFinal" => "3023" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/12810557" "web" => "Medline" ] ] ] ] ] ] ] ] 39 => array:3 [ "identificador" => "bib0040" "etiqueta" => "40" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Biomarkers of stress in rats exercised in swimming at intensities equal and superior to the maximal estable lactate phase" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => "RVL Contarteze" 1 => "FB Manchado" 2 => "CA Gobatto" 3 => "MAR de Mello" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:6 [ "tituloSerie" => "Rev Bras Med Esporte" "fecha" => "2007" "volumen" => "13" "numero" => "3" "paginaInicial" => "150e" "paginaFinal" => "154e" ] ] ] ] ] ] ] ] ] ] "agradecimientos" => array:1 [ 0 => array:4 [ "identificador" => "xack786276" "titulo" => "Acknowledgments" "texto" => "<p id="para0035" class="elsevierStylePara elsevierViewall">The authors would like to thank <span class="elsevierStyleGrantSponsor" id="gs0001">Fundação de Amparo à Pesquisa do Espírito Santo</span> (FAPES, grants numbers <span class="elsevierStyleGrantNumber" refid="gs0001">506/2020</span>, <span class="elsevierStyleGrantNumber" refid="gs0001">637/2022</span>, <span class="elsevierStyleGrantNumber" refid="gs0001">1007/2022</span>, <span class="elsevierStyleGrantNumber" refid="gs0001">2022-VT4KM</span>, <span class="elsevierStyleGrantNumber" refid="gs0001">2022-J72BB</span> and <span class="elsevierStyleGrantNumber" refid="gs0001">2022-5SBS</span>), <span class="elsevierStyleGrantSponsor" id="gs0002">Conselho Nacional de Desenvolvimento Científico e Tecnológico</span> (CNPq, grant number <span class="elsevierStyleGrantNumber" refid="gs0002">307749/2021-5</span>) and scientific assistance address to Carlos H. O. Reis. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.</p>" "vista" => "all" ] ] ] "idiomaDefecto" => "en" "url" => "/18075932/000000790000000C/v123_202411010523/S1807593224000887/v123_202411010523/en/main.assets" "Apartado" => array:4 [ "identificador" => "94924" "tipo" => "SECCION" "en" => array:2 [ "titulo" => "Original articles" "idiomaDefecto" => true ] "idiomaDefecto" => "en" ] "PDF" => "https://static.elsevier.es/multimedia/18075932/000000790000000C/v123_202411010523/S1807593224000887/v123_202411010523/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1807593224000887?idApp=UINPBA00004N" ]
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