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"cita" => "Enferm Infecc Microbiol Clin. 2017;35:283-6" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:2 [ "total" => 1824 "formatos" => array:3 [ "EPUB" => 16 "HTML" => 1311 "PDF" => 497 ] ] "en" => array:12 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>" "titulo" => "Frequency and molecular characterization of invasive isolates of <span class="elsevierStyleItalic">Streptococcus pneumoniae</span> serotypes 6C and 6D in Colombia" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => array:2 [ 0 => "en" 1 => "es" ] "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "283" "paginaFinal" => "286" ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Frecuencia y caracterización molecular de aislamientos invasivos de <span class="elsevierStyleItalic">Streptococcus pneumoniae</span> serotipos 6C y 6D en Colombia" ] ] "contieneResumen" => array:2 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"nombre" => "Diego" "apellidos" => "Domingo" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 4 => array:3 [ "nombre" => "Laura" "apellidos" => "Llorca" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 5 => array:3 [ "nombre" => "Ana" "apellidos" => "Correa" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 6 => array:3 [ "nombre" => "Manuel" "apellidos" => "López-Brea" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] ] "afiliaciones" => array:4 [ 0 => array:3 [ "entidad" => "Servicio de Microbiologia, Hospital Universitario de la Princesa, Instituto de Investigación Sanitaria Princesa (IIS-IP), Madrid, Spain" "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Departamento de Medicina Preventiva, Salud Pública y Microbiologia, Facultad de Medicina, Universidad Autónoma de Madrid, Spain" "etiqueta" => "b" "identificador" => "aff0010" ] 2 => array:3 [ "entidad" => "Unidad de Gastroenterología Pediátrica, Hospital Universitario Doce de Octubre, <span class="elsevierStyleItalic">Instituto</span> de <span class="elsevierStyleItalic">Investigación</span> Hospital <span class="elsevierStyleItalic">12 de Octubre</span>, Madrid, Spain" "etiqueta" => "c" "identificador" => "aff0015" ] 3 => array:3 [ "entidad" => "Unidad de Gastroenterología Pediátrica, Hospital Universitario Niño Jesus, Instituto de Investigación Sanitaria Princesa (IIS-IP), Madrid, Spain" "etiqueta" => "d" "identificador" => "aff0020" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Sensibilidad de aislamientos clínicos de <span class="elsevierStyleItalic">Helicobacter pylori</span> a seis antimicrobianos utilizando los criterios EUCAST y comparando los resultados con criterios utilizados anteriormente" ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 2701 "Ancho" => 2542 "Tamanyo" => 361966 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Distribution of antibiotic MICs for <span class="elsevierStyleItalic">H. pylori</span> clinical isolates. MICs were determined by E-Test and adjusted to the highest two-fold dilution values. MICs of amoxicillin (A) tetracycline (B), metronidazole (C), clarithromycin (D), rifampicin (E) and levofloxacin (F) are shown. Filled arrows indicate the EUCAST resistance breakpoints and dashed arrows the previously used breakpoints. Exe Y shows the number of <span class="elsevierStyleItalic">H. pylori</span> isolates for each MIC value and Exe X shows each MIC value (mg/L). Arrows indicates the EUCAST (<elsevierMultimedia ident="201804050444524911"></elsevierMultimedia>) or previous (<elsevierMultimedia ident="201804050444524912"></elsevierMultimedia>) resistance breakpoint.</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Helicobacter pylori</span> is a Gram-negative spiral rod colonizing the gastric mucosa mainly at the antrum, producing gastric inflammation. Patient could remain without symptomatology for his o her whole life or develop several pathologies such as duodenal or gastric ulcer; mucosa-associated lymphoid tissue (MALT) or gastric cancer.<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">1</span></a> Association with non-digestive diseases has also been described.<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">2</span></a> When treatment is needed, several triple or quadruple therapies could be used. Amoxicillin, tetracycline, metronidazole and clarithromycin are the antimicrobials most frequently used combined with proton pump inhibitors or bismuth salts.<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">3</span></a> Several papers have stressed the importance of doing <span class="elsevierStyleItalic">H. pylori</span> susceptibility testing before administering the treatment.<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">4</span></a> However, different methodology could be performed for <span class="elsevierStyleItalic">in vitro</span> susceptibility testing. Several Societies and Committees have defined reference methods and breakpoints for categorized organisms as susceptible or resistant to antimicrobial agents.</p><p id="par0010" class="elsevierStylePara elsevierViewall">The National Committee for Clinical Laboratory Standard (NCCLS) (now the Clinical and Laboratory Standards Institute, CLSI) proposed in 1999 and continued recommending <span class="elsevierStyleItalic">H. pylori</span> breakpoints only for clarithromycin and using an agar dilution.<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">5</span></a> The British Society for Antimicrobial Chemotherapy proposed the Epsilometer test (E-test)<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">6</span></a> and recommended breakpoints for four antimicrobials. Several studies conducted by the European <span class="elsevierStyleItalic">Helicobacter pylori</span> Study Group also used the E-test and proposed breakpoints for six antimicrobials.<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">7</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">The European Committee for Antimicrobial Susceptibility Testing (EUCAST) was initiated by the European Society of Clinical Microbiology and Infectious Diseases (ESCMID) to harmonize minimum inhibitory concentration (MIC) breakpoints across Europe.<a class="elsevierStyleCrossRef" href="#bib0145"><span class="elsevierStyleSup">8</span></a> In March–April 2011 the EUCAST group proposed breakpoints for six antimicrobials used to treat <span class="elsevierStyleItalic">H. pylori.</span><a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">9</span></a> The breakpoints are based on the epidemiological cut-off value (ECOFF), on clinical validation or on the study of resistance mechanisms.</p><p id="par0020" class="elsevierStylePara elsevierViewall">The adoption of new guidelines or changes in breakpoints can have an important effect on antimicrobial-resistance surveillance.<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">10</span></a></p><p id="par0025" class="elsevierStylePara elsevierViewall">The aim of this study was to determine the resistance percentage in <span class="elsevierStyleItalic">H. pylori</span> clinical isolates using EUCAST breakpoints comparing the results obtained with others previously used. MIC value distribution in <span class="elsevierStyleItalic">H. pylori</span> clinical isolates was also studied.</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Patients and <span class="elsevierStyleItalic">H. pylori</span> strains</span><p id="par0030" class="elsevierStylePara elsevierViewall">824 <span class="elsevierStyleItalic">H. pylori</span> strains were isolated from antral gastric mucosal biopsy specimens obtained from symptomatic patients from January 1, 2007 to December 2014. 641 (77.8%) were children (median age 8.99<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>3.3) and 183 (22.2%) were adults (median age 43.1<span class="elsevierStyleHsp" style=""></span>±<span class="elsevierStyleHsp" style=""></span>15.9). 59.1% were females and 46.8% were males. No data of previous eradication treatment were available.</p><p id="par0035" class="elsevierStylePara elsevierViewall">Biopsies were cultured in selective and non-selective media obtained commercially (Blood-supplemented Columbia Agar plates and Pylori agar, BioMerieux). <span class="elsevierStyleItalic">H. pylori</span> strains were cultured under microaerobic conditions obtained in an anaerobiosis jar with a microaerobic gas-generating envelopment. Strains were identified by colony and Gram stain morphology, and urease, oxidase and catalase positive test. From November 2012, any strain with a rare resistance was confirmed to be <span class="elsevierStyleItalic">H. pylori</span> by MALDI-TOF.</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Susceptibility methods</span><p id="par0040" class="elsevierStylePara elsevierViewall">Susceptibility to amoxicillin (AMX), tetracycline (TET), metronidazole (MET), clarithromycin (CLA), rifampicin (RIF) and levofloxacin (LEV) was performed by the E-test.<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">7</span></a> The bacteria were subcultured for 48<span class="elsevierStyleHsp" style=""></span>h in Blood-supplemented Columbia agar and a bacterial suspension adjusted to 10<span class="elsevierStyleSup">7</span><span class="elsevierStyleHsp" style=""></span>CFU/mL was inoculated directly onto Mueller-Hinton agar supplemented with 5% sheep blood obtained commercially (bioMerieux).</p><p id="par0045" class="elsevierStylePara elsevierViewall">E-test was applied over the culture media within 30<span class="elsevierStyleHsp" style=""></span>min of inoculation. Plates containing the E-test were incubated under microaerobic atmosphere. After 72<span class="elsevierStyleHsp" style=""></span>h of incubation, the Minimal Inhibitory Concentration (MIC) was determined by considering the point were elipse growth cut with the scale number in the E-test strip.</p><p id="par0050" class="elsevierStylePara elsevierViewall">To analyze the data each MIC value was adjusted to the next higher twofold dilutions (as studied by broth microdilution or agar dilution).</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Interpretative criteria</span><p id="par0055" class="elsevierStylePara elsevierViewall">The MICs obtained in the entire period were analyzed according to the breakpoints previously used and to EUCAST breakpoints (<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>).<a class="elsevierStyleCrossRefs" href="#bib0130"><span class="elsevierStyleSup">5,6,9,11,12</span></a></p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Statistical analysis</span><p id="par0060" class="elsevierStylePara elsevierViewall">95% Confidence intervals (95%CI) of prevalence rates were calculated. Data were analyzed using EpiInfo 6.04 (CDC, USA) computer software.</p></span></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Results</span><p id="par0065" class="elsevierStylePara elsevierViewall">The percentage of strains that are susceptible, intermediate or resistant by using the EUCAST breakpoints and the previously used breakpoints is in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>. The overall percentage of resistance to the 6 antibiotics by the new EUCAST breakpoints and by the previous breakpoints was 8.5% and 3.2% for amoxicillin, 0.6% and 0.1% for tetracycline, 39.2% and 39.7% for metronidazole, 51.2% and 51.2% for clarithromycin, 32% and 3.1% for rifampicin and 6.7% and 6.7% for levofloxacin.</p><p id="par0070" class="elsevierStylePara elsevierViewall">The distributions of MICs for amoxicillin, tetracycline, metronidazole, clarithromycin, rifampicin and levofloxacin in <span class="elsevierStyleItalic">H. pylori</span> clinical isolates are shown in <a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>.</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Discussion</span><p id="par0075" class="elsevierStylePara elsevierViewall">Several triple or quadruple therapies are recommended for treatment of <span class="elsevierStyleItalic">H. pylori</span> infection, those being omeprazole, clarithromycin and amoxicillin which are the most frequently used. However, infection by a clarithromycin-resistant strain is a risk factor for treatment failure<a class="elsevierStyleCrossRef" href="#bib0170"><span class="elsevierStyleSup">13</span></a> and other antimicrobial agents used are tetracycline, metronidazole, levofloxacin or rifabutin.<a class="elsevierStyleCrossRef" href="#bib0175"><span class="elsevierStyleSup">14</span></a> Treatment for <span class="elsevierStyleItalic">H. pylori</span> infection is usually started on an empirical basis, and when an infecting strain is resistant to the antimicrobial agents used, its successful eradication is hampered.</p><p id="par0080" class="elsevierStylePara elsevierViewall">The amoxicillin breakpoint, proposed by EUCAST, was based on the epidemiological cut-off value although no evidence exists which determines whether treatment is successful for infections caused by isolates with MICs >0.12<span class="elsevierStyleHsp" style=""></span>mg/L. Metronidazole cut-off was the current and widely accepted breakpoint, but there is no clinical validation. Clarithromycin breakpoints have been clinically validated and isolates with MIC above 0.5<span class="elsevierStyleHsp" style=""></span>mg/L have a resistance mechanism (23S RNA mutation). Tetracycline breakpoint correlates with mutations in 16S RNA, levofloxacin breakpoint with <span class="elsevierStyleItalic">gyrA</span> mutations, and rifampicin breakpoint with <span class="elsevierStyleItalic">rpoB</span> mutation, although there are no outcome data and there is no clinical validation.<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">9</span></a></p><p id="par0085" class="elsevierStylePara elsevierViewall">EUCAST guidelines for antimicrobial susceptibility testing (AST) are being implemented in European Laboratories.<a class="elsevierStyleCrossRef" href="#bib0180"><span class="elsevierStyleSup">15</span></a> Adoption of new guidelines and breakpoints can have a significant effect on reports and prior to implementing new guidelines, the consequences should be considered to prevent misunderstandings in interpretations. In this study AST's interpretation of the EUCAST breakpoint compared with the previous one was investigated and EUCAST will lead to significantly more isolates of <span class="elsevierStyleItalic">H. pylori</span> being resistant to rifampicin and amoxicillin. However, there are no important changes with the rest of the antimicrobial agents used for treatment of this infection, A total of 8.5% of the strains were amoxicillin-resistant when using the EUCAST breakpoints compared with 3.2% for the previous breakpoints. Amoxicillin is included in most treatments, such as sequential, concomitant and hybrid treatment; resistance to this antibiotic is clinically important and requires attention. On the other hand, there are no clinical data to confirm if strains with MIC of 0.25–0.5<span class="elsevierStyleHsp" style=""></span>mg/L, although they are amoxicillin-resistant according to EUCAST breakpoints, could be eliminated with oral amoxicillin of 1<span class="elsevierStyleHsp" style=""></span>g/12<span class="elsevierStyleHsp" style=""></span>h. In our study most of the amoxicillin-resistant strains had low MICs.</p><p id="par0090" class="elsevierStylePara elsevierViewall">In the study done by Kim et al.<a class="elsevierStyleCrossRef" href="#bib0185"><span class="elsevierStyleSup">16</span></a> no amoxicillin breakpoint was used to categorized the strains, although 5.6% of strains studied in 1994 and 18.5% of the strains studied in 2003 had MIC >0.5<span class="elsevierStyleHsp" style=""></span>mg/L. Moreover, two of these strains in 1994 and 3 in 2003 had an MIC of 8<span class="elsevierStyleHsp" style=""></span>mg/L. Wu et al.<a class="elsevierStyleCrossRef" href="#bib0190"><span class="elsevierStyleSup">17</span></a> reported a surprisingly high prevalence of amoxicillin resistance using a breakpoint of >0.5<span class="elsevierStyleHsp" style=""></span>mg/L, 71.9%, with 115 out of 153 being higher than 0.125 and 44 strains with MIC >16<span class="elsevierStyleHsp" style=""></span>mg/l.</p><p id="par0095" class="elsevierStylePara elsevierViewall">An increase of the resistance to tetracycline has been described by Kim et al.<a class="elsevierStyleCrossRef" href="#bib0185"><span class="elsevierStyleSup">16</span></a> reporting a resistance rate of a 5.9% in 1987 and 12.3% in 2003 with MICs >4<span class="elsevierStyleHsp" style=""></span>mg/L. Moreover, Wu et al.<a class="elsevierStyleCrossRef" href="#bib0190"><span class="elsevierStyleSup">17</span></a> reported a surprisingly high prevalence of tetracycline resistance, 58.8% by using a breakpoint of >16, with 104 out of 153 being higher than 1 and 79 strains with MIC >32<span class="elsevierStyleHsp" style=""></span>mg/l.</p><p id="par0100" class="elsevierStylePara elsevierViewall">Metronidazole has been widely prescribed for infections such as parasitic or female genital infections and could contribute to the high resistance rate found everywhere.</p><p id="par0105" class="elsevierStylePara elsevierViewall">In a European Study using the new EUCAST breakpoints, <span class="elsevierStyleItalic">H. pylori</span> resistance rates for adults were 17.5% for clarithromycin, 14.1% for levofloxacin and 34.9% for metronidazole, and were higher for clarithromycin and levofloxacin in Western/Central and Southern Europe (resistance was higher than 20%) than those in Northern European countries (resistance lower than 10%).<a class="elsevierStyleCrossRef" href="#bib0195"><span class="elsevierStyleSup">18</span></a> Moreover, an association was found between outpatient quinolone use and the proportion of levofloxacin resistance and between the use of long-acting macrolides and clarithromycin resistance.</p><p id="par0110" class="elsevierStylePara elsevierViewall">MIC distribution of the strains tested according to the MIC value was bimodal for metronidazole and clarithromycin, whilst a continuous distribution was observed for amoxicillin, tetracycline, rifampicin and levofloxacin. The wild type population is the subpopulation of isolates with no detectable acquired resistance mechanisms,<a class="elsevierStyleCrossRefs" href="#bib0200"><span class="elsevierStyleSup">19,20</span></a> but the mechanism of resistance was not studied herein.</p><p id="par0115" class="elsevierStylePara elsevierViewall">There is a need for continuous surveillance of resistance to antimicrobial agents in <span class="elsevierStyleItalic">H. pylori</span> infections as well as in other infections. The knowledge of the breakpoints used in each study is mandatory when comparing data obtained from different studies.</p><p id="par0120" class="elsevierStylePara elsevierViewall">Recently Boyanova et al.<a class="elsevierStyleCrossRef" href="#bib0210"><span class="elsevierStyleSup">21</span></a> compared EUCAST with previously used breakpoint in 299 strains in Bulgaria and found similar results: although there were differences for 3 of the antibiotics tested, the differences in the resistant percentage was lower than 4%. Unfortunately, rifampicin resistance is determined only with EUCAST.</p><p id="par0125" class="elsevierStylePara elsevierViewall">According to the results of this study, no matter which breakpoint was used, similar results were found for the antibiotics with high clinical relevance, whilst there were differences with amoxicillin and rifampicin.</p></span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Conflict of interest</span><p id="par0130" class="elsevierStylePara elsevierViewall">None declared.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:11 [ 0 => array:3 [ "identificador" => "xres1012321" "titulo" => "Abstract" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abst0005" "titulo" => "Introduction" ] 1 => array:2 [ "identificador" => "abst0010" "titulo" => "Methods" ] 2 => array:2 [ "identificador" => "abst0015" "titulo" => "Results" ] 3 => array:2 [ "identificador" => "abst0020" "titulo" => "Conclusions" ] ] ] 1 => array:2 [ "identificador" => "xpalclavsec971488" "titulo" => "Keywords" ] 2 => array:3 [ "identificador" => "xres1012322" "titulo" => "Resumen" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abst0025" "titulo" => "Introducción" ] 1 => array:2 [ "identificador" => "abst0030" "titulo" => "Métodos" ] 2 => array:2 [ "identificador" => "abst0035" "titulo" => "Resultados" ] 3 => array:2 [ "identificador" => "abst0040" "titulo" => "Conclusión" ] ] ] 3 => array:2 [ "identificador" => "xpalclavsec971489" "titulo" => "Palabras clave" ] 4 => array:2 [ "identificador" => "sec0005" "titulo" => "Introduction" ] 5 => array:3 [ "identificador" => "sec0010" "titulo" => "Methods" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "sec0015" "titulo" => "Patients and H. pylori strains" ] 1 => array:2 [ "identificador" => "sec0020" "titulo" => "Susceptibility methods" ] 2 => array:2 [ "identificador" => "sec0025" "titulo" => "Interpretative criteria" ] 3 => array:2 [ "identificador" => "sec0030" "titulo" => "Statistical analysis" ] ] ] 6 => array:2 [ "identificador" => "sec0035" "titulo" => "Results" ] 7 => array:2 [ "identificador" => "sec0040" "titulo" => "Discussion" ] 8 => array:2 [ "identificador" => "sec0045" "titulo" => "Conflict of interest" ] 9 => array:2 [ "identificador" => "xack341476" "titulo" => "Acknowledgement" ] 10 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2015-11-17" "fechaAceptado" => "2016-02-09" "PalabrasClave" => array:2 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec971488" "palabras" => array:3 [ 0 => "Clarithromycin resistance" 1 => "EUCAST breakpoints" 2 => "Multi-resistance" ] ] ] "es" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec971489" "palabras" => array:3 [ 0 => "<span class="elsevierStyleItalic">Helicobacter pylori</span>" 1 => "Resistencia a Claritromicina" 2 => "Punto de corte EUCAST" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "en" => array:3 [ "titulo" => "Abstract" "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Introduction</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">The aim of this study was to determine the differences in percentage resistance in <span class="elsevierStyleItalic">H. pylori</span> clinical isolates using EUCAST breakpoints compared with previously used breakpoints. MIC value distribution in <span class="elsevierStyleItalic">H. pylori</span> clinical isolates was also studied.</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">Susceptibility to amoxicillin, tetracycline, metronidazole, clarithromycin, rifampicin and levofloxacin was performed by E-test in 824 <span class="elsevierStyleItalic">H. pylori</span> clinical isolates. EUCAST and previous breakpoints defined resistance as follows: MIC >0.12<span class="elsevierStyleHsp" style=""></span>mg/L and ≥2<span class="elsevierStyleHsp" style=""></span>mg/L for amoxicillin, >8<span class="elsevierStyleHsp" style=""></span>mg/L and ≥8<span class="elsevierStyleHsp" style=""></span>mg/L for metronidazole, >0.5<span class="elsevierStyleHsp" style=""></span>mg/L and ≥1<span class="elsevierStyleHsp" style=""></span>mg/L for clarithromycin, >1<span class="elsevierStyleHsp" style=""></span>mg/L and ≥32<span class="elsevierStyleHsp" style=""></span>mg/L for rifampicin, and >1<span class="elsevierStyleHsp" style=""></span>mg/L and ≥4<span class="elsevierStyleHsp" style=""></span>mg/L for tetracycline and >1<span class="elsevierStyleHsp" style=""></span>mg/L levofloxacin.</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Overall resistance rate by EUCAST and by previous breakpoints was 8.5% and 3.2% for amoxicillin, 0.6% and 0.1% for tetracycline, 39.2% and 39.7% for metronidazole, 51.2% and 51.2% for clarithromycin, 32% and 3.1% for rifampicin, and 6.7% and 6.7% for levofloxacin.</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">When using the different breakpoints for antimicrobial susceptibility testing, similar results were found with most antibiotics tested (tetracycline, metronidazole, clarithromycin, and levofloxacin), except for amoxicillin and rifampicin.</p></span>" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abst0005" "titulo" => "Introduction" ] 1 => array:2 [ "identificador" => "abst0010" "titulo" => "Methods" ] 2 => array:2 [ "identificador" => "abst0015" "titulo" => "Results" ] 3 => array:2 [ "identificador" => "abst0020" "titulo" => "Conclusions" ] ] ] "es" => array:3 [ "titulo" => "Resumen" "resumen" => "<span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Introducción</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">El objetivo de este estudio era determinar las diferencias en el porcentaje de resistencia de aislamientos clínicos de <span class="elsevierStyleItalic">H. pylori</span> usando los puntos de corte de EUCAST comparado con los puntos de corte usados anteriormente. También se estudió la distribución de los valores de CMI en los aislamientos de <span class="elsevierStyleItalic">H. pylori</span>.</p></span> <span id="abst0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Métodos</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">La sensibilidad de amoxicilina, tetraciclina, metronidazol, claritromicina, rifampicina y levo-floxacina se determinó mediante E-test en 824 aislamientos clínicos de <span class="elsevierStyleItalic">H. pylori</span>. Los puntos de corte utilizados fueron EUCAST: CMI >0,12<span class="elsevierStyleHsp" style=""></span>mg/L para amoxicilina, >8<span class="elsevierStyleHsp" style=""></span>mg/L para metronidazol, >0,5<span class="elsevierStyleHsp" style=""></span>mg/L para claritromicina y >1<span class="elsevierStyleHsp" style=""></span>mg/L para rifampicina, tetraciclina y levofloxacina. Los puntos de corte que se habían utilizado antes de EUCAST fueron: CMI ≥2<span class="elsevierStyleHsp" style=""></span>mg/L para amoxicilina, ≥8<span class="elsevierStyleHsp" style=""></span>mg/L para metronidazol, ≥1<span class="elsevierStyleHsp" style=""></span>mg/L para claritromicina, ≥32<span class="elsevierStyleHsp" style=""></span>mg/L para rifampicina, ≥4<span class="elsevierStyleHsp" style=""></span>mg/L para tetraciclina y >1<span class="elsevierStyleHsp" style=""></span>mg/L para levofloxacina.</p></span> <span id="abst0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Resultados</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">La resistencia global con los puntos de corte EUCAST y con los puntos de corte anteriores fue: 8,5% y 3,2% para amoxicilina, 0,6% y 0,1% para tetraciclina, 39,2% y 39,7% para metronidazol, 51,2% y 51,2% para claritromicina, 32% y 3,1% para rifampicina y 6,7% y 6,7% para levofloxacina.</p></span> <span id="abst0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Conclusión</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">A pesar de la utilización de diferentes puntos de corte, se obtuvieron resultados de resistencia similares para la mayoría de los antibióticos probados (tetraciclina, metronidazol, claritrnnñomicina, y levofloxacino), con la única excepción de amoxicilina y rifampicina.</p></span>" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "abst0025" "titulo" => "Introducción" ] 1 => array:2 [ "identificador" => "abst0030" "titulo" => "Métodos" ] 2 => array:2 [ "identificador" => "abst0035" "titulo" => "Resultados" ] 3 => array:2 [ "identificador" => "abst0040" "titulo" => "Conclusión" ] ] ] ] "NotaPie" => array:1 [ 0 => array:2 [ "etiqueta" => "☆" "nota" => "<p class="elsevierStyleNotepara" id="npar0025">This work has enjoyed the cooperation of the Research Fund Health FIS 08/1775 (Institute of Health Carlos III, Ministry of Economy and Competitiveness).</p>" ] ] "multimedia" => array:4 [ 0 => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 2701 "Ancho" => 2542 "Tamanyo" => 361966 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Distribution of antibiotic MICs for <span class="elsevierStyleItalic">H. pylori</span> clinical isolates. MICs were determined by E-Test and adjusted to the highest two-fold dilution values. MICs of amoxicillin (A) tetracycline (B), metronidazole (C), clarithromycin (D), rifampicin (E) and levofloxacin (F) are shown. Filled arrows indicate the EUCAST resistance breakpoints and dashed arrows the previously used breakpoints. Exe Y shows the number of <span class="elsevierStyleItalic">H. pylori</span> isolates for each MIC value and Exe X shows each MIC value (mg/L). Arrows indicates the EUCAST (<elsevierMultimedia ident="201804050444524911"></elsevierMultimedia>) or previous (<elsevierMultimedia ident="201804050444524912"></elsevierMultimedia>) resistance breakpoint.</p>" ] ] 1 => array:8 [ "identificador" => "tbl0005" "etiqueta" => "Table 1" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "detalles" => array:1 [ 0 => array:3 [ "identificador" => "at1" "detalle" => "Table " "rol" => "short" ] ] "tabla" => array:3 [ "leyenda" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">S<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>susceptible, I<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>Intermediate, R<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>resistant, ns<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>no significant.</p>" "tablatextoimagen" => array:1 [ 0 => array:2 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head " align="" valign="top" scope="col"> \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " colspan="4" align="center" valign="top" scope="col" style="border-bottom: 2px solid black">EUCAST Breakpoints</th><th class="td" title="table-head " colspan="4" align="center" valign="top" scope="col" style="border-bottom: 2px solid black">Previous Breakpoints</th><th class="td" title="table-head " align="left" valign="top" scope="col"><span class="elsevierStyleItalic">P</span> value \t\t\t\t\t\t\n \t\t\t\t</th></tr><tr title="table-row"><th class="td" title="table-head " align="" valign="top" scope="col" style="border-bottom: 2px solid black"> \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Breakpoint<span class="elsevierStyleSup">9</span> S/R (MIC in mg/L) \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">% S (CI) \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">% I (CI) \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">% R (CI) \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Breakpoint S/R (MIC in mg/L) \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">% S (CI) \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">% I (CI) \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">% R (CI) \t\t\t\t\t\t\n \t\t\t\t</th><th class="td" title="table-head " align="" valign="top" scope="col" style="border-bottom: 2px solid black"> \t\t\t\t\t\t\n \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">AMX \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><0.12/>0.12 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">91.5% (89.1–93.3%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="" valign="top"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">8.5% (6.6–11%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><1/>2<a class="elsevierStyleCrossRef" href="#tblfn0005"><span class="elsevierStyleSup">a</span></a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">96.8% (95.2–97.8%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">0.4% (0.1–1.2%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">3.2% (2.1–5.4.7%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><0.01 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">TET \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><1/>1 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">99.4% (98.5–99.7%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="" valign="top"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.6% (0.2–1.5%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><2/>4<a class="elsevierStyleCrossRef" href="#tblfn0005"><span class="elsevierStyleSup">a</span></a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">99.9% (99.1–99.9%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="" valign="top"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">0.1% (0.02–0.8%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">ns \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">MET \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><8/>8 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">60.8% (57.4–64%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="" valign="top"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">39.2% (35.9–42.5%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><4/>8<a class="elsevierStyleCrossRef" href="#tblfn0005"><span class="elsevierStyleSup">a</span></a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">60.3% (56.9–63.6%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">0.2% (0.1–0.8%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">39.7% (36.3–43%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">ns \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">CLA \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><0.25/>0.5 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">48.2% (44.8–51.6%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">0.6% (0.2–1.4%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">51.2% (47.7–54.5%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><0.25/>1<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">b</span></a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">48.2% (44.8–52%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">0.9% (0.4–1.9%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">51.2% (47.7–54.5%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">ns \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">RIF \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><1/>1 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">68% (64.4 –71.2%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="" valign="top"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">32% (28.7–35.5%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><32/>32<a class="elsevierStyleCrossRef" href="#tblfn0015"><span class="elsevierStyleSup">c</span></a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">96.9% (95.3–97.9%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="" valign="top"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">3.1% (2–4.6%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><0.01 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td-with-role" title="table-entry ; entry_with_role_rowhead " align="left" valign="top">LEV \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><1/>1 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">93.3% (91.3–94.9%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="" valign="top"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">6.7% (5–8.7%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top"><1/>1<a class="elsevierStyleCrossRef" href="#tblfn0020"><span class="elsevierStyleSup">d</span></a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">93.3% (91.3–94.9%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="" valign="top"> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="char" valign="top">6.7% (5–8.7%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="table-entry " align="left" valign="top">ns \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] "imagenFichero" => array:1 [ 0 => "xTab1714370.png" ] ] ] "notaPie" => array:4 [ 0 => array:3 [ "identificador" => "tblfn0005" "etiqueta" => "a" "nota" => "<p class="elsevierStyleNotepara" id="npar0005">BSAC breakpoints.<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">6</span></a></p>" ] 1 => array:3 [ "identificador" => "tblfn0010" "etiqueta" => "b" "nota" => "<p class="elsevierStyleNotepara" id="npar0010">CLSI and BSAC breakpoint.<a class="elsevierStyleCrossRefs" href="#bib0130"><span class="elsevierStyleSup">5,6</span></a></p>" ] 2 => array:3 [ "identificador" => "tblfn0015" "etiqueta" => "c" "nota" => "<p class="elsevierStyleNotepara" id="npar0015">From Fujimura et al.<a class="elsevierStyleCrossRef" href="#bib0160"><span class="elsevierStyleSup">11</span></a></p>" ] 3 => array:3 [ "identificador" => "tblfn0020" "etiqueta" => "d" "nota" => "<p class="elsevierStyleNotepara" id="npar0020">From Wueppenhorst et al.<a class="elsevierStyleCrossRef" href="#bib0165"><span class="elsevierStyleSup">12</span></a></p>" ] ] ] "descripcion" => array:1 [ "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">Assignment of <span class="elsevierStyleItalic">H. pylori</span> clinical isolates to interpretative categories according to the EUCAST breakpoint and the previous breakpoint (percentage and confidence interval, CI). 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Year/Month | Html | Total | |
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2024 November | 16 | 1 | 17 |
2024 October | 70 | 12 | 82 |
2024 September | 111 | 11 | 122 |
2024 August | 91 | 9 | 100 |
2024 July | 87 | 13 | 100 |
2024 June | 68 | 13 | 81 |
2024 May | 93 | 20 | 113 |
2024 April | 76 | 28 | 104 |
2024 March | 62 | 13 | 75 |
2024 February | 81 | 12 | 93 |
2024 January | 116 | 20 | 136 |
2023 December | 127 | 23 | 150 |
2023 November | 110 | 47 | 157 |
2023 October | 136 | 35 | 171 |
2023 September | 85 | 25 | 110 |
2023 August | 75 | 13 | 88 |
2023 July | 84 | 53 | 137 |
2023 June | 88 | 39 | 127 |
2023 May | 107 | 13 | 120 |
2023 April | 64 | 11 | 75 |
2023 March | 84 | 13 | 97 |
2023 February | 72 | 4 | 76 |
2023 January | 68 | 7 | 75 |
2022 December | 56 | 20 | 76 |
2022 November | 63 | 28 | 91 |
2022 October | 52 | 20 | 72 |
2022 September | 56 | 22 | 78 |
2022 August | 74 | 14 | 88 |
2022 July | 50 | 13 | 63 |
2022 June | 53 | 16 | 69 |
2022 May | 50 | 22 | 72 |
2022 April | 46 | 27 | 73 |
2022 March | 58 | 23 | 81 |
2022 February | 50 | 12 | 62 |
2022 January | 86 | 12 | 98 |
2021 December | 65 | 22 | 87 |
2021 November | 75 | 47 | 122 |
2021 October | 65 | 26 | 91 |
2021 September | 64 | 23 | 87 |
2021 August | 78 | 19 | 97 |
2021 July | 61 | 19 | 80 |
2021 June | 49 | 12 | 61 |
2021 May | 78 | 16 | 94 |
2021 April | 206 | 20 | 226 |
2021 March | 84 | 16 | 100 |
2021 February | 85 | 13 | 98 |
2021 January | 77 | 18 | 95 |
2020 December | 64 | 13 | 77 |
2020 November | 56 | 15 | 71 |
2020 October | 43 | 12 | 55 |
2020 September | 75 | 21 | 96 |
2020 August | 76 | 25 | 101 |
2020 July | 48 | 12 | 60 |
2020 June | 47 | 17 | 64 |
2020 May | 69 | 21 | 90 |
2020 April | 52 | 10 | 62 |
2020 March | 88 | 20 | 108 |
2020 February | 73 | 24 | 97 |
2020 January | 71 | 35 | 106 |
2019 December | 75 | 38 | 113 |
2019 November | 53 | 13 | 66 |
2019 October | 60 | 11 | 71 |
2019 September | 58 | 7 | 65 |
2019 August | 55 | 6 | 61 |
2019 July | 50 | 23 | 73 |
2019 June | 92 | 40 | 132 |
2019 May | 264 | 86 | 350 |
2019 April | 107 | 35 | 142 |
2019 March | 41 | 22 | 63 |
2019 February | 30 | 19 | 49 |
2019 January | 20 | 13 | 33 |
2018 December | 25 | 13 | 38 |
2018 November | 40 | 4 | 44 |
2018 October | 36 | 27 | 63 |
2018 September | 32 | 4 | 36 |
2018 August | 14 | 4 | 18 |
2018 July | 12 | 3 | 15 |
2018 June | 14 | 6 | 20 |
2018 May | 13 | 6 | 19 |
2018 April | 224 | 1 | 225 |
2018 March | 15 | 0 | 15 |
2018 February | 7 | 1 | 8 |
2018 January | 15 | 0 | 15 |
2017 December | 12 | 2 | 14 |
2017 November | 20 | 1 | 21 |
2017 October | 18 | 0 | 18 |
2017 September | 2 | 0 | 2 |
2017 July | 2 | 0 | 2 |
2017 June | 4 | 9 | 13 |
2017 May | 80 | 81 | 161 |
2017 April | 1 | 0 | 1 |
2017 March | 2 | 0 | 2 |
2017 February | 17 | 9 | 26 |
2017 January | 7 | 6 | 13 |
2016 December | 3 | 11 | 14 |
2016 November | 3 | 13 | 16 |
2016 October | 10 | 8 | 18 |
2016 September | 6 | 8 | 14 |
2016 August | 4 | 8 | 12 |
2016 July | 1 | 4 | 5 |