metricas
covid
Buscar en
Enfermedades Infecciosas y Microbiología Clínica (English Edition)
Toda la web
Inicio Enfermedades Infecciosas y Microbiología Clínica (English Edition) Synergistic anti-malarial effects of Ocimum sanctum leaf extract and artemisinin
Journal Information

Statistics

Follow this link to access the full text of the article

Scientific letter
Synergistic anti-malarial effects of Ocimum sanctum leaf extract and artemisinin
Efectos antipalúdicos sinérgicos del extracto de hoja de Ocimum sanctum y artemisinina
Zulvikar Syambani Ulhaqa,b
a Research Center for Pre-Clinical and Clinical Medicine, National Research and Innovation Agency Republic of Indonesia, Cibinong, Indonesia
b Department of Biochemistry, Faculty of Medicine and Health Sciences, Maulana Malik Ibrahim Islamic State University of Malang, Batu, East Java, Indonesia
Read
174
Times
was read the article
43
Total PDF
131
Total HTML
Share statistics
 array:23 [
  "pii" => "S2529993X23000187"
  "issn" => "2529993X"
  "doi" => "10.1016/j.eimce.2022.06.016"
  "estado" => "S300"
  "fechaPublicacion" => "2023-04-01"
  "aid" => "2618"
  "copyright" => "Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica"
  "copyrightAnyo" => "2022"
  "documento" => "simple-article"
  "crossmark" => 1
  "subdocumento" => "crp"
  "cita" => "Enferm Infecc Microbiol Clin. 2023;41:250-1"
  "abierto" => array:3 [
    "ES" => false
    "ES2" => false
    "LATM" => false
  ]
  "gratuito" => false
  "lecturas" => array:1 [
    "total" => 0
  ]
  "itemSiguiente" => array:19 [
    "pii" => "S2529993X23000771"
    "issn" => "2529993X"
    "doi" => "10.1016/j.eimce.2023.02.002"
    "estado" => "S300"
    "fechaPublicacion" => "2023-04-01"
    "aid" => "2616"
    "copyright" => "Sociedad Española de Enfermedades Infecciosas y Microbiología Clínica"
    "documento" => "article"
    "crossmark" => 1
    "subdocumento" => "crp"
    "cita" => "Enferm Infecc Microbiol Clin. 2023;41:251-3"
    "abierto" => array:3 [
      "ES" => false
      "ES2" => false
      "LATM" => false
    ]
    "gratuito" => false
    "lecturas" => array:1 [
      "total" => 0
    ]
    "en" => array:11 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Scientific letter</span>"
      "titulo" => "High rates of colonization by <span class="elsevierStyleItalic">Staphylococcus aureus</span> in medical students before their clinical practices"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "251"
          "paginaFinal" => "253"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "es" => array:1 [
          "titulo" => "Elevadas tasas de colonizaci&#243;n por <span class="elsevierStyleItalic">Staphylococcus aureus</span> en los estudiantes de medicina antes de realizar sus pr&#225;cticas cl&#237;nicas"
        ]
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "resumenGrafico" => array:2 [
        "original" => 0
        "multimedia" => array:8 [
          "identificador" => "fig0005"
          "etiqueta" => "Figure 1"
          "tipo" => "MULTIMEDIAFIGURA"
          "mostrarFloat" => true
          "mostrarDisplay" => false
          "figura" => array:1 [
            0 => array:4 [
              "imagen" => "gr1.jpeg"
              "Alto" => 1362
              "Ancho" => 3183
              "Tamanyo" => 214924
            ]
          ]
          "detalles" => array:1 [
            0 => array:3 [
              "identificador" => "at0030"
              "detalle" => "Figure "
              "rol" => "short"
            ]
          ]
          "descripcion" => array:1 [
            "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Distribution of the rates of <span class="elsevierStyleItalic">Staphylococcus aureus</span> carriers according to the academic year and location &#40;nasal&#44; pharyngeal and both&#41;&#46;</p> <p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">&#42;Total percentage of students colonized at least in the nose&#46;</p>"
          ]
        ]
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Esther R&#237;os, Iciar Rodr&#237;guez-Avial, Esther Culebras, Carmen Rodr&#237;guez-Avial"
          "autores" => array:4 [
            0 => array:2 [
              "nombre" => "Esther"
              "apellidos" => "R&#237;os"
            ]
            1 => array:2 [
              "nombre" => "Iciar"
              "apellidos" => "Rodr&#237;guez-Avial"
            ]
            2 => array:2 [
              "nombre" => "Esther"
              "apellidos" => "Culebras"
            ]
            3 => array:2 [
              "nombre" => "Carmen"
              "apellidos" => "Rodr&#237;guez-Avial"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "Traduccion" => array:1 [
      "es" => array:9 [
        "pii" => "S0213005X22001458"
        "doi" => "10.1016/j.eimc.2022.06.003"
        "estado" => "S300"
        "subdocumento" => ""
        "abierto" => array:3 [
          "ES" => false
          "ES2" => false
          "LATM" => false
        ]
        "gratuito" => false
        "lecturas" => array:1 [
          "total" => 0
        ]
        "idiomaDefecto" => "es"
        "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0213005X22001458?idApp=UINPBA00004N"
      ]
    ]
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2529993X23000771?idApp=UINPBA00004N"
    "url" => "/2529993X/0000004100000004/v2_202304072117/S2529993X23000771/v2_202304072117/en/main.assets"
  ]
  "itemAnterior" => array:19 [
    "pii" => "S2529993X23000783"
    "issn" => "2529993X"
    "doi" => "10.1016/j.eimce.2023.02.003"
    "estado" => "S300"
    "fechaPublicacion" => "2023-04-01"
    "aid" => "2619"
    "copyright" => "Sociedad Espa&#241;ola de Enfermedades Infecciosas y Microbiolog&#237;a Cl&#237;nica"
    "documento" => "article"
    "crossmark" => 1
    "subdocumento" => "crp"
    "cita" => "Enferm Infecc Microbiol Clin. 2023;41:248-50"
    "abierto" => array:3 [
      "ES" => false
      "ES2" => false
      "LATM" => false
    ]
    "gratuito" => false
    "lecturas" => array:1 [
      "total" => 0
    ]
    "en" => array:11 [
      "idiomaDefecto" => true
      "cabecera" => "<span class="elsevierStyleTextfn">Scientific letter</span>"
      "titulo" => "Phenotypic and genotypic characterization of <span class="elsevierStyleItalic">Shigella sonnei</span> carrying the extended-spectrum beta-lactamase CTX-M-27&#46; A report of two cases in Spain in men who have sex with men"
      "tienePdf" => "en"
      "tieneTextoCompleto" => "en"
      "paginas" => array:1 [
        0 => array:2 [
          "paginaInicial" => "248"
          "paginaFinal" => "250"
        ]
      ]
      "titulosAlternativos" => array:1 [
        "es" => array:1 [
          "titulo" => "Caracterizaci&#243;n fenot&#237;pica y genot&#237;pica de <span class="elsevierStyleItalic">Shigella sonnei</span> portadora de la betalactamasa de espectro extendido CTX-M-27&#46; A prop&#243;sito de dos casos en Espa&#241;a en hombres que tienen sexo con hombres"
        ]
      ]
      "contieneTextoCompleto" => array:1 [
        "en" => true
      ]
      "contienePdf" => array:1 [
        "en" => true
      ]
      "resumenGrafico" => array:2 [
        "original" => 0
        "multimedia" => array:8 [
          "identificador" => "fig0005"
          "etiqueta" => "Figure 1"
          "tipo" => "MULTIMEDIAFIGURA"
          "mostrarFloat" => true
          "mostrarDisplay" => false
          "figura" => array:1 [
            0 => array:4 [
              "imagen" => "gr1.jpeg"
              "Alto" => 926
              "Ancho" => 2101
              "Tamanyo" => 259157
            ]
          ]
          "detalles" => array:1 [
            0 => array:3 [
              "identificador" => "at0005"
              "detalle" => "Figure "
              "rol" => "short"
            ]
          ]
          "descripcion" => array:1 [
            "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Identification of <span class="elsevierStyleItalic">Shigella</span> spp&#46; and detection of the presence of an extended-spectrum beta-lactamase &#40;ESBL&#41;&#46;</p> <p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">&#40;A&#41; Left&#58; TSI &#40;triple sugar-iron&#41; medium&#44; glucose fermentation can be seen but not producing gas&#59; right&#58; LIA &#40;lysine-iron agar&#41; medium&#44; there is no decarboxylation or deamination of the lysine&#46; &#40;B&#41; Disc-diffusion technique with the confirmation kits &#34;Total ESBL&#44; AmpC and ESBL<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>AmpC Confirm kit&#34; &#40;ROSCO Diagnostica A&#47;S&#44; Taastrup&#44; Denmark&#41;&#46; The increase in the cefotaxime and ceftazidime halo &#40;&#62;5<span class="elsevierStyleHsp" style=""></span>mm&#41; can be seen in the presence of clavulanic acid but not in cloxacillin&#44; confirming the presence of ESBL&#46;</p>"
          ]
        ]
      ]
      "autores" => array:1 [
        0 => array:2 [
          "autoresLista" => "Domingo Fern&#225;ndez Vecilla, Kristina Zugazaga Inchaurza, Itxaso Lombide Aguirre, Jos&#233; Luis D&#237;az de Tuesta del Arco"
          "autores" => array:4 [
            0 => array:2 [
              "nombre" => "Domingo"
              "apellidos" => "Fern&#225;ndez Vecilla"
            ]
            1 => array:2 [
              "nombre" => "Kristina"
              "apellidos" => "Zugazaga Inchaurza"
            ]
            2 => array:2 [
              "nombre" => "Itxaso"
              "apellidos" => "Lombide Aguirre"
            ]
            3 => array:2 [
              "nombre" => "Jos&#233; Luis"
              "apellidos" => "D&#237;az de Tuesta del Arco"
            ]
          ]
        ]
      ]
    ]
    "idiomaDefecto" => "en"
    "Traduccion" => array:1 [
      "es" => array:9 [
        "pii" => "S0213005X22001483"
        "doi" => "10.1016/j.eimc.2022.06.006"
        "estado" => "S300"
        "subdocumento" => ""
        "abierto" => array:3 [
          "ES" => false
          "ES2" => false
          "LATM" => false
        ]
        "gratuito" => false
        "lecturas" => array:1 [
          "total" => 0
        ]
        "idiomaDefecto" => "es"
        "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0213005X22001483?idApp=UINPBA00004N"
      ]
    ]
    "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2529993X23000783?idApp=UINPBA00004N"
    "url" => "/2529993X/0000004100000004/v2_202304072117/S2529993X23000783/v2_202304072117/en/main.assets"
  ]
  "en" => array:14 [
    "idiomaDefecto" => true
    "cabecera" => "<span class="elsevierStyleTextfn">Scientific letter</span>"
    "titulo" => "Synergistic anti-malarial effects of <span class="elsevierStyleItalic">Ocimum sanctum</span> leaf extract and artemisinin"
    "tieneTextoCompleto" => true
    "paginas" => array:1 [
      0 => array:2 [
        "paginaInicial" => "250"
        "paginaFinal" => "251"
      ]
    ]
    "autores" => array:1 [
      0 => array:3 [
        "autoresLista" => "Zulvikar Syambani Ulhaq"
        "autores" => array:1 [
          0 => array:4 [
            "nombre" => "Zulvikar Syambani"
            "apellidos" => "Ulhaq"
            "email" => array:2 [
              0 => "zulvikar.syambani.ulhaq@brin.go.id"
              1 => "zulhaq@kedokteran.uin-malang.ac.id"
            ]
            "referencia" => array:2 [
              0 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">a</span>"
                "identificador" => "aff0005"
              ]
              1 => array:2 [
                "etiqueta" => "<span class="elsevierStyleSup">b</span>"
                "identificador" => "aff0010"
              ]
            ]
          ]
        ]
        "afiliaciones" => array:2 [
          0 => array:3 [
            "entidad" => "Research Center for Pre-Clinical and Clinical Medicine&#44; National Research and Innovation Agency Republic of Indonesia&#44; Cibinong&#44; Indonesia"
            "etiqueta" => "a"
            "identificador" => "aff0005"
          ]
          1 => array:3 [
            "entidad" => "Department of Biochemistry&#44; Faculty of Medicine and Health Sciences&#44; Maulana Malik Ibrahim Islamic State University of Malang&#44; Batu&#44; East Java&#44; Indonesia"
            "etiqueta" => "b"
            "identificador" => "aff0010"
          ]
        ]
      ]
    ]
    "titulosAlternativos" => array:1 [
      "es" => array:1 [
        "titulo" => "Efectos antipal&#250;dicos sin&#233;rgicos del extracto de hoja de <span class="elsevierStyleItalic">Ocimum sanctum</span> y artemisinina"
      ]
    ]
    "resumenGrafico" => array:2 [
      "original" => 0
      "multimedia" => array:7 [
        "identificador" => "fig0005"
        "etiqueta" => "Fig&#46; 1"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr1.jpeg"
            "Alto" => 2693
            "Ancho" => 1676
            "Tamanyo" => 242102
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Synergistic anti-malarial effects of <span class="elsevierStyleItalic">Ocimum sanctum</span> leaf extract and artemisinin&#46; &#40;A&#41; Reduction of parasitemia&#46; &#40;B&#41; TGF-&#946; level by peritoneal macrophages during mice malaria infections measured by ELISA after 24<span class="elsevierStyleHsp" style=""></span>h <span class="elsevierStyleItalic">in vitro</span> culture&#46; Data are expressed as a mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>standard deviation &#40;SD&#41;&#46; &#40;&#42;&#41; Indicates a significant difference compared to the baseline level or between two groups indicated in the graphs &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#41;&#46; OS1<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">O&#46; Sanctum</span> 0&#46;25<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day&#59; OS2<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">O&#46; Sanctum</span> 0&#46;5<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day&#59; ART<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>artemisinin 0&#46;036<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day&#59; ART<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>OS1<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>artemisinin 0&#46;036<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">O&#46; Sanctum</span> 0&#46;25<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day&#59; ART<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>OS2<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>artemisinin 0&#46;036<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">O&#46; Sanctum</span> 0&#46;5<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day&#59; NC<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>negative control&#59; PC&#44; positive control&#46;</p>"
        ]
      ]
    ]
    "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall">Malaria remains a major health problem in Indonesia&#46; It has been reported that the prevalence of parasitemia in Timika&#44; Papua was 16&#46;3&#37;&#44; and almost 50&#37; of cases were caused by <span class="elsevierStyleItalic">Plasmodium falciparum</span> &#40;<span class="elsevierStyleItalic">P&#46; falciparum</span>&#41;&#46;<a class="elsevierStyleCrossRef" href="#bib0060"><span class="elsevierStyleSup">1</span></a> Papua province has not only the highest prevalence of malaria in Indonesia but also the highest prevalence of multidrug-resistance to both <span class="elsevierStyleItalic">P&#46; vivax</span> and <span class="elsevierStyleItalic">P&#46; falciparum</span>&#46;<a class="elsevierStyleCrossRef" href="#bib0065"><span class="elsevierStyleSup">2</span></a> Although artemisinin-based combination therapy &#40;ACT&#41; was adopted as the first-line anti-malarial treatment&#44; studies have demonstrated the failure of ACT towards malaria elimination in several Southeast Asian countries&#46;<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">3</span></a> Hence&#44; an alternative combination therapy against malaria is needed&#46;</p><p id="par0010" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Ocimum sanctum</span> &#40;<span class="elsevierStyleItalic">O&#46; sanctum</span>&#44; Indonesian name Kemangi&#41; belongs to the Lamiaceae family and is widely distributed throughout Indonesia&#46; <span class="elsevierStyleItalic">O&#46; sanctum</span> is used by Indonesian to treat several diseases&#44; including malaria&#59; however&#44; the underlying mechanism remains elusive&#46; A study revealed that the ethanolic leaf extract of <span class="elsevierStyleItalic">O&#46; sanctum</span> displays more potent antiplasmodial activity than other Ocimum species <span class="elsevierStyleItalic">in vitro</span>&#46;<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">4</span></a> Therefore&#44; this study aims to evaluate the synergistic anti-malarial properties of <span class="elsevierStyleItalic">O&#46; sanctum</span> and artemisinin &#40;ART&#41; against the <span class="elsevierStyleItalic">Plasmodium</span> infection <span class="elsevierStyleItalic">in vivo</span>&#46; In addition&#44; the level of transforming growth factor-beta &#40;TGF-&#946;&#41; was examined&#46;</p><p id="par0015" class="elsevierStylePara elsevierViewall">The leaves of <span class="elsevierStyleItalic">O&#46; sanctum</span> were collected from Malang&#44; East Java&#44; Indonesia&#46; The species was identified and confirmed by a plant taxonomist of the herbarium unit&#44; UPT Materia Medica&#44; Batu&#44; East Java&#44; Indonesia&#46; The ethanolic extract preparation was conducted as previously described&#46;<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">5</span></a> For <span class="elsevierStyleItalic">in vivo</span> experiments&#44; female Balb&#47;c mice between 8 and 12 weeks of age were used&#46; Sixty-three mice were randomly assigned into seven groups &#40;9 mice per group&#41;&#44; namely negative control&#44; positive control&#44; infected mice treated with ART &#40;0&#46;036<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day&#41;&#59; two different doses of <span class="elsevierStyleItalic">O&#46; Sanctum</span> extract &#40;0&#46;25 and 0&#46;5<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day&#41;&#59; and combinations of artemisinin and <span class="elsevierStyleItalic">O&#46; Sanctum</span> extract&#46; The malaria model was performed by i&#46;p&#46; injection of <span class="elsevierStyleItalic">Plasmodium berghei</span> adjusted to 10<span class="elsevierStyleSup">6</span> parasites in 0&#46;2<span class="elsevierStyleHsp" style=""></span>mL blood per mouse&#46; Infected mice were then treated on the sixth day of infection with parasitemia approximately around 10&#37; for seven consecutive days&#46; To examine TGF-&#946; levels&#44; mice peritoneal macrophages on day seven post-treatment were cultured as described previously&#44;<a class="elsevierStyleCrossRef" href="#bib0085"><span class="elsevierStyleSup">6</span></a> and the supernatants were used to quantify the concentration of TGF-&#946; &#40;BioLegend&#41; by ELISA&#46;<a class="elsevierStyleCrossRefs" href="#bib0090"><span class="elsevierStyleSup">7&#8211;10</span></a> The study was approved by the medical ethics committee of Brawijaya University with Reference No&#46; 27-KE&#46; The reduction of parasitemia and TGF-&#946; levels were analyzed by two and one-way ANOVA&#44; respectively&#44; followed by the Fisher LSD <span class="elsevierStyleItalic">post hoc</span> test using StatPlus&#46; Significant differences were accepted when <span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#46;</p><p id="par0020" class="elsevierStylePara elsevierViewall">The reduction of parasitemia was observed in all groups started on day five of the treatment&#46; Notably&#44; the administration of ART and <span class="elsevierStyleItalic">O&#46; Sanctum</span> extract at a dose of 0&#46;5<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day demonstrated a higher effectivity to speed up <span class="elsevierStyleItalic">Plasmodium</span> clearance than other groups &#40;at day 3 compared to the baseline level&#44; <a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>A&#41;&#46; Moreover&#44; the suppression of TGF-&#946; was only observed in mice treated with combination therapy but not with monotherapy &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>B&#41;&#44; thereby implying that combination therapy exhibits synergistic anti-malarial effects towards <span class="elsevierStyleItalic">Plasmodium</span> elimination&#46; Various active constituents have been identified in the ethanolic extract of <span class="elsevierStyleItalic">O&#46; Sanctum&#44;</span> such as alkaloids&#44; glycosides&#44; flavonoids&#44; phenols&#44; saponins&#44; tannins&#44; steroids&#44; and triterpenoids&#46;<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">4</span></a> The possible mechanisms of <span class="elsevierStyleItalic">O&#46; Sanctum</span> extract in eliminating <span class="elsevierStyleItalic">P&#46; berghei</span> may have occurred through the inhibition of hemozoin biocrystallization&#44; protein synthesis&#44; or &#946;-haematin formation&#44; stimulation of DNA fragmentation&#44; and cytoplasmic acidification&#46;<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">4</span></a></p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0025" class="elsevierStylePara elsevierViewall">In line with previous findings&#44; this study showed that the upregulation of TGF-&#946; levels was observed in <span class="elsevierStyleItalic">P&#46; berghei</span> infected mice&#46; Furthermore&#44; the upregulation of TGF-&#946; is known to be associated with the risk of complicated malaria&#46;<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">11</span></a> These results imply that TGF-&#946; is linked to the disease severity of malaria&#46; Therefore&#44; a treatment that modulates the suppression of TGF-&#946; would be beneficial to minimize malaria progression&#46; In summary&#44; the combination of ART and the ethanolic extract of <span class="elsevierStyleItalic">O&#46; Sanctum</span> displays synergistic anti-plasmodial activity <span class="elsevierStyleItalic">in vivo</span>&#46; Further studies are warranted to investigate the potential of <span class="elsevierStyleItalic">O&#46; Sanctum</span> as an alternative ACT regimen in clinical settings&#46;</p><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0005">Authors&#8217; contribution</span><p id="par0030" class="elsevierStylePara elsevierViewall">Z&#46;S&#46;U&#46; conceived&#44; performed&#44; analyzed&#44; and wrote the article&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Funding</span><p id="par0035" class="elsevierStylePara elsevierViewall">N&#47;A&#46;</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Conflict of interest</span><p id="par0040" class="elsevierStylePara elsevierViewall">None to declare&#46;</p></span></span>"
    "textoCompletoSecciones" => array:1 [
      "secciones" => array:4 [
        0 => array:2 [
          "identificador" => "sec0005"
          "titulo" => "Authors&#8217; contribution"
        ]
        1 => array:2 [
          "identificador" => "sec0010"
          "titulo" => "Funding"
        ]
        2 => array:2 [
          "identificador" => "sec0015"
          "titulo" => "Conflict of interest"
        ]
        3 => array:1 [
          "titulo" => "References"
        ]
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "multimedia" => array:1 [
      0 => array:7 [
        "identificador" => "fig0005"
        "etiqueta" => "Fig&#46; 1"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr1.jpeg"
            "Alto" => 2693
            "Ancho" => 1676
            "Tamanyo" => 242102
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Synergistic anti-malarial effects of <span class="elsevierStyleItalic">Ocimum sanctum</span> leaf extract and artemisinin&#46; &#40;A&#41; Reduction of parasitemia&#46; &#40;B&#41; TGF-&#946; level by peritoneal macrophages during mice malaria infections measured by ELISA after 24<span class="elsevierStyleHsp" style=""></span>h <span class="elsevierStyleItalic">in vitro</span> culture&#46; Data are expressed as a mean<span class="elsevierStyleHsp" style=""></span>&#177;<span class="elsevierStyleHsp" style=""></span>standard deviation &#40;SD&#41;&#46; &#40;&#42;&#41; Indicates a significant difference compared to the baseline level or between two groups indicated in the graphs &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#41;&#46; OS1<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">O&#46; Sanctum</span> 0&#46;25<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day&#59; OS2<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">O&#46; Sanctum</span> 0&#46;5<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day&#59; ART<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>artemisinin 0&#46;036<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day&#59; ART<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>OS1<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>artemisinin 0&#46;036<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">O&#46; Sanctum</span> 0&#46;25<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day&#59; ART<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>OS2<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>artemisinin 0&#46;036<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">O&#46; Sanctum</span> 0&#46;5<span class="elsevierStyleHsp" style=""></span>mg&#47;g&#47;day&#59; NC<span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>negative control&#59; PC&#44; positive control&#46;</p>"
        ]
      ]
    ]
    "bibliografia" => array:2 [
      "titulo" => "References"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "bibs0015"
          "bibliografiaReferencia" => array:11 [
            0 => array:3 [
              "identificador" => "bib0060"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The risk of morbidity and mortality following recurrent malaria in Papua&#44; Indonesia&#58; a retrospective cohort study"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "S&#46; Dini"
                            1 => "N&#46;M&#46; Douglas"
                            2 => "J&#46;R&#46; Poespoprodjo"
                            3 => "E&#46; Kenangalem"
                            4 => "P&#46; Sugiarto"
                            5 => "I&#46;D&#46; Plumb"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1186/s12916-020-1497-0"
                      "Revista" => array:5 [
                        "tituloSerie" => "BMC Med"
                        "fecha" => "2020"
                        "volumen" => "18"
                        "paginaInicial" => "28"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/32075649"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib0065"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Multidrug-resistant <span class="elsevierStyleItalic">Plasmodium vivax</span> associated with severe and fatal malaria&#58; a prospective study in Papua&#44; Indonesia"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "E&#46; Tjitra"
                            1 => "N&#46;M&#46; Anstey"
                            2 => "P&#46; Sugiarto"
                            3 => "N&#46; Warikar"
                            4 => "E&#46; Kenangalem"
                            5 => "M&#46; Karyana"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1371/journal.pmed.0050128"
                      "Revista" => array:5 [
                        "tituloSerie" => "PLoS Med"
                        "fecha" => "2008"
                        "volumen" => "5"
                        "paginaInicial" => "e128"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18563962"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0070"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Plasmodium falciparum resistance to artemisinin-based combination therapies&#58; a sword of Damocles in the path toward malaria elimination"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "M&#46; Ouji"
                            1 => "J&#46;M&#46; Augereau"
                            2 => "L&#46; Paloque"
                            3 => "F&#46; Benoit-Vical"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:4 [
                        "tituloSerie" => "Parasite Paris Fr"
                        "fecha" => "2018"
                        "volumen" => "25"
                        "paginaInicial" => "24"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib0075"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "In vitro antiplasmodial effect of ethanolic extracts of traditional medicinal plant <span class="elsevierStyleItalic">Ocimum</span> species against <span class="elsevierStyleItalic">Plasmodium falciparum</span>"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "S&#46;J&#46; Inbaneson"
                            1 => "R&#46; Sundaram"
                            2 => "P&#46; Suganthi"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Asian Pac J Trop Med"
                        "fecha" => "2012"
                        "volumen" => "5"
                        "paginaInicial" => "103"
                        "paginaFinal" => "106"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib0080"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Antibacterial activity of <span class="elsevierStyleItalic">Citrus hystrix</span> toward <span class="elsevierStyleItalic">Salmonella</span> spp&#46; infection"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "Z&#46;S&#46; Ulhaq"
                            1 => "T&#46;H&#46; Hendyatama"
                            2 => "F&#46; Hameed"
                            3 => "D&#46; Santosaningsih"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Enfermedades Infecc Microbiol Clin Engl Ed"
                        "fecha" => "2021"
                        "volumen" => "39"
                        "paginaInicial" => "283"
                        "paginaFinal" => "286"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib0085"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The isolation and characterization of murine macrophages"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "X&#46; Zhang"
                            1 => "R&#46; Goncalves"
                            2 => "D&#46;M&#46; Mosser"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:3 [
                        "tituloSerie" => "Curr Protoc Immunol"
                        "fecha" => "2008"
                        "paginaInicial" => "1"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib0090"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Association between serum interleukin-6 levels with the risk and clinical severity of primary open-angle glaucoma"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "Z&#46;S&#46; Ulhaq"
                            1 => "Y&#46;T&#46;N&#46; Hasan"
                            2 => "L&#46;N&#46; Rachma"
                            3 => "G&#46;V&#46; Soraya"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Expert Rev Ophthalmol"
                        "fecha" => "2021"
                        "volumen" => "16"
                        "paginaInicial" => "505"
                        "paginaFinal" => "510"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            7 => array:3 [
              "identificador" => "bib0095"
              "etiqueta" => "8"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Serum IL-6&#47;IL-10 ratio as a biomarker for the diagnosis and severity assessment of primary-open angle glaucoma"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "Z&#46;S&#46; Ulhaq"
                            1 => "G&#46;V&#46; Soraya"
                            2 => "Y&#46;T&#46;N&#46; Hasan"
                            3 => "L&#46;N&#46; Rachma"
                            4 => "E&#46; Rachmawati"
                            5 => "S&#46; Shodry"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:2 [
                        "tituloSerie" => "Eur J Ophthalmol"
                        "fecha" => "2021"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib0100"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Comparative performance of ELISA and dot blot assay for TSH-receptor antibody detection in Graves&#8217; disease"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "Z&#46; Zulkarnain"
                            1 => "Z&#46;S&#46; Ulhaq"
                            2 => "H&#46; Sujuti"
                            3 => "D&#46;W&#46; Soeatmadji"
                            4 => "H&#46; Zufry"
                            5 => "D&#46;K&#46; Wuragil"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1002/jcla.24288"
                      "Revista" => array:5 [
                        "tituloSerie" => "J Clin Lab Anal"
                        "fecha" => "2022"
                        "volumen" => "36"
                        "paginaInicial" => "e24288"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/35187713"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib0105"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The 40-kDa protein of <span class="elsevierStyleItalic">Lumbricus rubellus</span> eradicates methicillin-resistant <span class="elsevierStyleItalic">Staphylococcusaureus</span> in an long-term nasal carriage model"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "Z&#46;S&#46; Ulhaq"
                            1 => "A&#46;S&#46;K&#46; Putri"
                            2 => "W&#46;P&#46;S&#46; Atmaja"
                            3 => "D&#46; Santosaningsih"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Enferm Infecc Microbiol Clin"
                        "fecha" => "2021"
                        "volumen" => "39"
                        "paginaInicial" => "310"
                        "paginaFinal" => "311"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib0110"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Dysregulation of cytokines expression in complicated falciparum malaria with increased TGF-&#946; and IFN-&#947; and decreased IL-2 and IL-12"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "S&#46;D&#46; Lourembam"
                            1 => "C&#46;E&#46; Sawian"
                            2 => "S&#46; Baruah"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Cytokine"
                        "fecha" => "2013"
                        "volumen" => "64"
                        "paginaInicial" => "503"
                        "paginaFinal" => "508"
                      ]
                    ]
                  ]
                ]
              ]
            ]
          ]
        ]
      ]
    ]
  ]
  "idiomaDefecto" => "en"
  "url" => "/2529993X/0000004100000004/v2_202304072117/S2529993X23000187/v2_202304072117/en/main.assets"
  "Apartado" => array:4 [
    "identificador" => "63562"
    "tipo" => "SECCION"
    "en" => array:2 [
      "titulo" => "Scientific letters"
      "idiomaDefecto" => true
    ]
    "idiomaDefecto" => "en"
  ]
  "PDF" => "https://static.elsevier.es/multimedia/2529993X/0000004100000004/v2_202304072117/S2529993X23000187/v2_202304072117/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/"
  "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2529993X23000187?idApp=UINPBA00004N"
]
Article information
ISSN: 2529993X
Original language: English
The statistics are updated each day
Year/Month Html Pdf Total
2024 November 2 1 3
2024 October 16 4 20
2024 September 16 5 21
2024 August 10 4 14
2024 July 14 3 17
2024 June 12 3 15
2024 May 7 5 12
2024 April 10 4 14
2024 March 9 1 10
2024 February 4 2 6
2024 January 4 3 7
2023 December 4 1 5
2023 November 9 1 10
2023 October 11 4 15
2023 September 3 2 5
Show all

Follow this link to access the full text of the article

es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos