Scientific letter
Coexistence of JAK2V617F mutation and isolated del(5q) in a patient with thrombocytosis
Coexistencia de la mutación JAK2V617F y la del(5q) aislada en un paciente con trombocitosis
a ICO Badalona-Hospital Germans Trias i Pujol, Institut de Recerca contra la Leucèmia Josep Carreras, UAB, Badalona, Spain
b Laboratori de Citologia Hematològica, Servei de Patologia, Hospital del Mar and GRETNHE, Cancer Research Program, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain
Read
3
Timeswas read the article
2
Total PDF
1
Total HTML
Share statistics
array:24 [ "pii" => "S2387020621002539" "issn" => "23870206" "doi" => "10.1016/j.medcle.2020.04.048" "estado" => "S300" "fechaPublicacion" => "2021-06-25" "aid" => "5320" "copyright" => "Elsevier España, S.L.U.. All rights reserved" "copyrightAnyo" => "2020" "documento" => "simple-article" "crossmark" => 1 "subdocumento" => "crp" "cita" => "Med Clin. 2021;156:625-7" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "Traduccion" => array:1 [ "en" => array:19 [ "pii" => "S0025775320304875" "issn" => "00257753" "doi" => "10.1016/j.medcli.2020.04.065" "estado" => "S300" "fechaPublicacion" => "2021-06-25" "aid" => "5320" "copyright" => "Elsevier España, S.L.U." "documento" => "simple-article" "crossmark" => 1 "subdocumento" => "crp" "cita" => "Med Clin. 2021;156:625-7" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "en" => array:11 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Scientific letter</span>" "titulo" => "Coexistence of JAK2V617F mutation and isolated del(5q) in a patient with thrombocytosis" "tienePdf" => "en" "tieneTextoCompleto" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "625" "paginaFinal" => "627" ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Coexistencia de la mutación JAK2V617F y la del(5q) aislada en un paciente con trombocitosis" ] ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Fig. 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 3480 "Ancho" => 3166 "Tamanyo" => 773100 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Hyperlobulated nuclei megakaryocytes with great size typical of the essential thrombocytemia (A) and small monolobated nuclei megakaryocytes, characteristic of the MDS isolated del(5q) (B) present in the bone marrow aspirate. Immunohistochemistry techniques in the bone marrow biopsy showed the presence of monolobated nuclei megakaryocytes with CD61 staining (C) and grade II fibrosis with reticulin stain (D), both highlighted with arrows. JAK2V617F mutation by ASO-PCR (E) and fluorescence in situ hybridization (FISH) (F) demonstrating the absence of del(5q) (two control signals in 5p15.2 and two signals for 5q31 region) in all the red colonies studied.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Silvia Marcé, Neus Ruiz-Xivillé, Leonor Arenillas" "autores" => array:3 [ 0 => array:2 [ "nombre" => "Silvia" "apellidos" => "Marcé" ] 1 => array:2 [ "nombre" => "Neus" "apellidos" => "Ruiz-Xivillé" ] 2 => array:2 [ "nombre" => "Leonor" "apellidos" => "Arenillas" ] ] ] ] ] "idiomaDefecto" => "en" "Traduccion" => array:1 [ "en" => array:9 [ "pii" => "S2387020621002539" "doi" => "10.1016/j.medcle.2020.04.048" "estado" => "S300" "subdocumento" => "" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2387020621002539?idApp=UINPBA00004N" ] ] "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0025775320304875?idApp=UINPBA00004N" "url" => "/00257753/0000015600000012/v1_202106110528/S0025775320304875/v1_202106110528/en/main.assets" ] ] "itemSiguiente" => array:18 [ "pii" => "S2387020621002436" "issn" => "23870206" "doi" => "10.1016/j.medcle.2020.05.037" "estado" => "S300" "fechaPublicacion" => "2021-06-25" "aid" => "5300" "copyright" => "Elsevier España, S.L.U." "documento" => "article" "crossmark" => 1 "subdocumento" => "crp" "cita" => "Med Clin. 2021;156:627-8" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "en" => array:10 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Scientific letter</span>" "titulo" => "Jaw osteonecrosis related to cabozantinib" "tienePdf" => "en" "tieneTextoCompleto" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "627" "paginaFinal" => "628" ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Osteonecrosis mandibular en relación al empleo de cabozantinib" ] ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "José Darío Sánchez López, Juan Andrés Rodríguez Ruiz, Miguel Pérez de Perceval Tara" "autores" => array:3 [ 0 => array:2 [ "nombre" => "José Darío" "apellidos" => "Sánchez López" ] 1 => array:2 [ "nombre" => "Juan Andrés" "apellidos" => "Rodríguez Ruiz" ] 2 => array:2 [ "nombre" => "Miguel" "apellidos" => "Pérez de Perceval Tara" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2387020621002436?idApp=UINPBA00004N" "url" => "/23870206/0000015600000012/v1_202106160926/S2387020621002436/v1_202106160926/en/main.assets" ] "itemAnterior" => array:18 [ "pii" => "S2387020621002576" "issn" => "23870206" "doi" => "10.1016/j.medcle.2020.11.020" "estado" => "S300" "fechaPublicacion" => "2021-06-25" "aid" => "5580" "copyright" => "Elsevier España, S.L.U." "documento" => "simple-article" "crossmark" => 1 "subdocumento" => "crp" "cita" => "Med Clin. 2021;156:622-4" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:1 [ "total" => 0 ] "en" => array:12 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Clinical report</span>" "titulo" => "May-Thurner syndrome and deep vein thrombosis: A series of 8 patients" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => array:2 [ 0 => "en" 1 => "es" ] "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "622" "paginaFinal" => "624" ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Síndrome de May-Thurner y trombosis venosa profunda: estudio de ocho pacientes" ] ] "contieneResumen" => array:2 [ "en" => true "es" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Alejandra García-García, Crhistian-Mario Oblitas, Francisco Galeano-Valle, Andrea Olmos-Nieto, Ana Ortega-Soudant, Pablo Demelo-Rodríguez" "autores" => array:6 [ 0 => array:2 [ "nombre" => "Alejandra" "apellidos" => "García-García" ] 1 => array:2 [ "nombre" => "Crhistian-Mario" "apellidos" => "Oblitas" ] 2 => array:2 [ "nombre" => "Francisco" "apellidos" => "Galeano-Valle" ] 3 => array:2 [ "nombre" => "Andrea" "apellidos" => "Olmos-Nieto" ] 4 => array:2 [ "nombre" => "Ana" "apellidos" => "Ortega-Soudant" ] 5 => array:2 [ "nombre" => "Pablo" "apellidos" => "Demelo-Rodríguez" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2387020621002576?idApp=UINPBA00004N" "url" => "/23870206/0000015600000012/v1_202106160926/S2387020621002576/v1_202106160926/en/main.assets" ] "en" => array:16 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Scientific letter</span>" "titulo" => "Coexistence of JAK2V617F mutation and isolated del(5q) in a patient with thrombocytosis" "tieneTextoCompleto" => true "saludo" => "Dear Editor," "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "625" "paginaFinal" => "627" ] ] "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "Silvia Marcé, Neus Ruiz-Xivillé, Leonor Arenillas" "autores" => array:3 [ 0 => array:4 [ "nombre" => "Silvia" "apellidos" => "Marcé" "email" => array:1 [ 0 => "smarce@iconcologia.net" ] "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">?</span>" "identificador" => "cor0005" ] ] ] 1 => array:3 [ "nombre" => "Neus" "apellidos" => "Ruiz-Xivillé" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 2 => array:3 [ "nombre" => "Leonor" "apellidos" => "Arenillas" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] ] ] ] "afiliaciones" => array:2 [ 0 => array:3 [ "entidad" => "ICO Badalona-Hospital Germans Trias i Pujol, Institut de Recerca contra la Leucèmia Josep Carreras, UAB, Badalona, Spain" "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Laboratori de Citologia Hematològica, Servei de Patologia, Hospital del Mar and GRETNHE, Cancer Research Program, IMIM (Hospital del Mar Medical Research Institute), Barcelona, Spain" "etiqueta" => "b" "identificador" => "aff0010" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "?" "correspondencia" => "Corresponding author." ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Coexistencia de la mutación JAK2V617F y la del(5q) aislada en un paciente con trombocitosis" ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Fig. 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 3480 "Ancho" => 3166 "Tamanyo" => 773100 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Hyperlobulated nuclei megakaryocytes with great size typical of the essential thrombocytemia (A) and small monolobated nuclei megakaryocytes, characteristic of the MDS isolated del(5q) (B) present in the bone marrow aspirate. Immunohistochemistry techniques in the bone marrow biopsy showed the presence of monolobated nuclei megakaryocytes with CD61 staining (C) and grade II fibrosis with reticulin stain (D), both highlighted with arrows. JAK2V617F mutation by ASO-PCR (E) and fluorescence in situ hybridization (FISH) (F) demonstrating the absence of del(5q) (two control signals in 5p15.2 and two signals for 5q31 region) in all the red colonies studied.</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall">Interstitial deletion of chromosome 5q is the most common cytogenetic abnormality in myelodisplastic syndromes (MDS). Patients with MDS isolated del(5q) have a refractory macrocytic anemia with or without other cytopenias and/or thrombocytosis, displastyc megakaryocytes and an indolent clinical course.<a class="elsevierStyleCrossRefs" href="#bib0030"><span class="elsevierStyleSup">1,2</span></a> Isolated del(5q) can occur also in myeloid malignancies including myeloproliferative neoplasms (MPN).<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">1</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">The JAK2V617F mutation is a frequent event in a large proportion of patients with MPN. Essential thrombocythemia (ET) is a MPN characterized by sustained thrombocytosis in the peripheral blood, increased number of large mature megakaryocytes in the bone marrow and by episodes of thrombosis and/or hemorrhage. Primary myelofibrosis (PMF) is the most frequent MPN associated with del(5q) and increased grade of reticulin fibrosis (grade III or more) in the bone marrow. However, JAK2V617F mutation is rare in other hematopoietic disorders, except in MDS with myelofibrosis, suggesting a myeloproliferative nature in a subset of MDS patients.<a class="elsevierStyleCrossRefs" href="#bib0040"><span class="elsevierStyleSup">3,4</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">Here we present an 82-year-old woman with persistent thrombocytosis and an increased proportion of megakaryocytes, some of them of big size and hyperlobulated nuclei, and others of small size and monolobated nuclei (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>A and B). Conventional cytogenetic revealed the presence of del(5)(q12;q33) and molecular studies demonstrated the JAK2V617F mutation. The biopsy obtained during the clinical course demonstrated augmented cellularity with decreased fat marrow and presence of grade II reticulin fibrosis with some focus of collagen fibrosis that suggests that it could be a consequence of the progression of an ET. It also showed a megakaryocytic hyperplasia with dysplastic features and monolobated nuclei megakaryocytes (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>C and D), postulating the existence of a MDS with isolated del(5q). Due to the suspicion of the coexistence of two hematological entities, we cultured in vitro erythroid burst-forming units (BFU-E) showing that 100% of the colonies harbored the JAK2V617F mutation. Simultaneously, fluorescence in situ hybridization (FISH) with a chromosome 5q31 deletion probe was also performed in these colonies and we could not demonstrate the 5q deletion in any of them (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>E and F), suggesting that both alterations may derive from genetically independent clones (MDS with isolated (5q) clone and NMP clone), and that only the most proliferative cells grew in the culture.</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0020" class="elsevierStylePara elsevierViewall">The concomitant presence of del(5q) and JAK2V617F mutation is a rare event which occurs prevalently in patients with a possibly MDS and MPN coexistence. Some studies investigated the clonal origin of JAK2V617F mutation in a patient with del(5q). Interestingly, they found that not all del(5q) cells were JAK2V617F positive suggesting that both alterations may derive from genetically independent clones. Ingram et al.<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">5</span></a> evidenced that JAK2V617F appeared earlier in these patients arising in a pluripotent stem cell and that del(5q) may be present in the same CD34+ cells though not all del(5q) cells are JAK2V617F positive. Sokol et al.<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">2</span></a> detected JAK2V617F mutant in two distinct multipotent stem cell progeny that lacked del(5q) indicating that JAK2V617F and del(5q) derived from independent clones, as we observed in our patient. Nevertheless, this does not exclude the possibility that both clones derived from a genetically unstable hierarchal stem cell. Cytogenetic and molecular genetics show overlaps in some MDS and MPN cases which indicate common pathways in the etiology of both disorders.<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">4</span></a> A literature review indicated that isolated deletion of 5q is uncommon in JAK2V617F positive ET but, when deletion is present, the disease often shows mixed features of both ET and MDS with isolated del(5q).<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">4</span></a></p><p id="par0025" class="elsevierStylePara elsevierViewall">In conclusion, BFU-E study may help to identify one neoplasm with two alterations or two genetically independent entities in cases with JAK2V617F and del(5q). The presence of del(5q) and JAK2V617F in patients combining both MDS and MPN requires further biological and clinical studies and longer follow-up to determine the prognosis of these patients. However, the combination of molecular, cytogenetic and colonies cell cultures studies in these patients may help to improve clinical management and biological knowledge.</p><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0005">Funding</span><p id="par0030" class="elsevierStylePara elsevierViewall">This work was supported in part by a grant from <span class="elsevierStyleGrantSponsor" id="gs1">Instituto de Salud Carlos III, Ministerio de Sanidad y Consumo</span>, Spain (PI 11/02519); <span class="elsevierStyleGrantSponsor" id="gs2">2014 SGR225 (GRE) Generalitat de Catalunya; Fundació Josep Carreras and Celgene Spain</span>.</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Conflict of interest</span><p id="par0035" class="elsevierStylePara elsevierViewall">Authors may describe any financial or personal relationship which can cause a conflict of interest regarding this article.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:4 [ 0 => array:2 [ "identificador" => "sec0005" "titulo" => "Funding" ] 1 => array:2 [ "identificador" => "sec0010" "titulo" => "Conflict of interest" ] 2 => array:2 [ "identificador" => "xack536465" "titulo" => "Acknowledgments" ] 3 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "multimedia" => array:1 [ 0 => array:7 [ "identificador" => "fig0005" "etiqueta" => "Fig. 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 3480 "Ancho" => 3166 "Tamanyo" => 773100 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Hyperlobulated nuclei megakaryocytes with great size typical of the essential thrombocytemia (A) and small monolobated nuclei megakaryocytes, characteristic of the MDS isolated del(5q) (B) present in the bone marrow aspirate. Immunohistochemistry techniques in the bone marrow biopsy showed the presence of monolobated nuclei megakaryocytes with CD61 staining (C) and grade II fibrosis with reticulin stain (D), both highlighted with arrows. JAK2V617F mutation by ASO-PCR (E) and fluorescence in situ hybridization (FISH) (F) demonstrating the absence of del(5q) (two control signals in 5p15.2 and two signals for 5q31 region) in all the red colonies studied.</p>" ] ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0015" "bibliografiaReferencia" => array:5 [ 0 => array:3 [ "identificador" => "bib0030" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "WHO classification of tumours of haematopoietic and lymphoid tissues" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "S.H. Swerdlow" 1 => "E. Campo" 2 => "N.L. Harris" 3 => "E.S. Jaffe" 4 => "S.A. Pileri" 5 => "H. Stein" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Libro" => array:4 [ "edicion" => "4th ed." "fecha" => "2017" "editorial" => "IARC Press" "editorialLocalizacion" => "Lyon, France" ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib0035" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "JAK2 (V617F) mutation in myelodysplastic syndrome (MDS) with del(5q) arises in genetically discordant clones" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "L. Sokol" 1 => "G. Caceres" 2 => "K. Rocha" 3 => "K.J. Stockero" 4 => "D.W. Dewald" 5 => "A.F. List" ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Leukemia Res" "fecha" => "2010" "volumen" => "34" "paginaInicial" => "821" "paginaFinal" => "823" ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bib0040" "etiqueta" => "3" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Clinical features and course of refractory anemia with ring sideroblasts associated with marked thrombocytosis" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "J. Broseus" 1 => "L. Florensa" 2 => "E. Zipperer" 3 => "S. Schnittger" 4 => "L. Malcovati" 5 => "S. Richebourg" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.3324/haematol.2011.053918" "Revista" => array:6 [ "tituloSerie" => "Haematologica" "fecha" => "2012" "volumen" => "97" "paginaInicial" => "1036" "paginaFinal" => "1041" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22532522" "web" => "Medline" ] ] ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bib0045" "etiqueta" => "4" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Distribution of cytogenetic abnormalities in myelodysplastic syndromes. Philadelphia negative myeloproliferative neoplasms, and the overlap MDS/MPN category" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => "U. Bacher" 1 => "S. Schnittger" 2 => "W. Kern" 3 => "T. Weiss" 4 => "T. Haferlach" 5 => "C. Haferlach" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1007/s00277-009-0745-3" "Revista" => array:6 [ "tituloSerie" => "Ann Hematol" "fecha" => "2009" "volumen" => "88" "paginaInicial" => "1207" "paginaFinal" => "1213" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19415278" "web" => "Medline" ] ] ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bib0050" "etiqueta" => "5" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The JAK2 V617F mutation identifies a subgroup of MDS patients with isolated 5q and a proliferative bone marrow" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => "W. Ingram" 1 => "N.C. Lea" 2 => "J. Cervera" 3 => "U. Germing" 4 => "P. Fenaux" 5 => "B. Cassinat" ] ] ] ] ] "host" => array:1 [ 0 => array:2 [ "doi" => "10.1038/sj.leu.2404215" "Revista" => array:6 [ "tituloSerie" => "Leukemia" "fecha" => "2006" "volumen" => "20" "paginaInicial" => "1319" "paginaFinal" => "1321" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16617322" "web" => "Medline" ] ] ] ] ] ] ] ] ] ] ] ] "agradecimientos" => array:1 [ 0 => array:4 [ "identificador" => "xack536465" "titulo" => "Acknowledgments" "texto" => "<p id="par0040" class="elsevierStylePara elsevierViewall">The authors would like to thank Diana Domínguez, Lurdes Zamora, Lourdes Florensa, Marta Cabezón, Alberto Pineda, Gustavo Tapia and José Tomás Navarro for their contribution to this publication.</p>" "vista" => "all" ] ] ] "idiomaDefecto" => "en" "url" => "/23870206/0000015600000012/v1_202106160926/S2387020621002539/v1_202106160926/en/main.assets" "Apartado" => array:4 [ "identificador" => "43311" "tipo" => "SECCION" "en" => array:2 [ "titulo" => "Scientific letters" "idiomaDefecto" => true ] "idiomaDefecto" => "en" ] "PDF" => "https://static.elsevier.es/multimedia/23870206/0000015600000012/v1_202106160926/S2387020621002539/v1_202106160926/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S2387020621002539?idApp=UINPBA00004N" ]
| Year/Month | Html | Total | |
|---|---|---|---|
| 2023 March | 1 | 2 | 3 |
