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Original article
Value of long non-coding RNA HAS2-AS1 as a diagnostic and prognostic marker of glioma
El valor de ARNncl HAS2-AS1 como marcador diagnóstico y pronóstico del glioma
A. Youa, J. Gua, J. Wanga, J. Lia, Y. Zhanga, G. Raoa, X. Gea, K. Zhanga, X. Gaob, D. Wanga,
Corresponding author
docwangdc1981@163.com

Corresponding author.
a The Fourth Department of Neurosurgery, Tangshan Gongren Hospital, 063000 Tangshan, China
b Operating Theatre, Tangshan Central Hospital, 063000 Tangshan, China
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    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Glioma is one of the most common primary tumors in the central nervous system&#44; accounting for about 40&#8211;50&#37; of primary intracranial tumors&#46; Glioma includes a variety of histologic features and the world health organization &#40;WHO&#41; has classified glioma using phenotypic and genotypic parameters since 2016 to make a more objective diagnosis of glioma&#46;<a class="elsevierStyleCrossRef" href="#bib0005"><span class="elsevierStyleSup">1</span></a> Furthermore&#44; the tumor is graded from the lowest to the highest malignancy degree &#40;I&#44; II&#44; III&#44; and IV&#41; according to its expected biological behavior&#46; Grade I is characterized by benign cytology&#44; grade II is characterized by medium cellularity without dysplasia or mitotic activity&#44; and grade III is characterized by cellularity with hypoplasia and mitosis&#44; grade IV is the same as grade III with additional proliferation and necrosis of microvessels&#46;<a class="elsevierStyleCrossRef" href="#bib0010"><span class="elsevierStyleSup">2</span></a> Besides grade I tumor can be cured by surgery&#44; the rest of the glioma is hard to be cured by the existing treatment&#44; and most patients experience relapse&#46; Generally&#44; patients with early diagnosis have a better prognosis&#44; with a 5-year survival rate of 42&#8211;92&#37;&#46;<a class="elsevierStyleCrossRefs" href="#bib0015"><span class="elsevierStyleSup">3&#8211;5</span></a></p><p id="par0010" class="elsevierStylePara elsevierViewall">It is found that one of the main reasons for the unsatisfactory survival of glioma after treatment is that currently&#44; the detection indexes for glioma are generally still on the patient&#39;s age and tumor histology&#44; leading to huge challenges in clinical prognosis&#44; treatment identification and early diagnosis of patients&#46;<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">6</span></a> Therefore&#44; a reliable biomarker for diagnosis and prognosis of glioma is urgently needed in clinic&#46; Long non-coding RNAs &#40;lncRNAs&#41; are a type of RNAs whose transcript length is greater than 200 nucleotides&#46; Studies have reported that lncRNAs can regulate gene expression at epigenetic&#44; transcriptional and post-transcriptional levels through chromosome modification&#44; transcriptional activation or interference&#46;<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a> Some studies have indicated that lncRNAs are closely related to tumors&#44; and there are significant differences in the expression of some lncRNAs in normal tissues and tumor tissues&#46; Abnormally expressed lncRNAs may play an important role in the occurrence of tumors&#44; and lncRNAs may become new markers for tumor diagnosis and evaluation of prognosis as well as therapeutic effect&#46;<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">8</span></a></p><p id="par0015" class="elsevierStylePara elsevierViewall">Hyaluronan &#40;HA&#41; is one of the commonest glycosaminoglycan &#40;GAG&#41; in extracellular matrix &#40;ECM&#41;&#44; wherein hyaluronan synthase 2 &#40;HAS2&#41; is the main HAS participating in HA synthesis&#46;<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">9</span></a> Recent reports pointed out that HAS2 antisense 1 &#40;HAS2-AS1&#41; is a kind of lncRNA belonging to natural antisense transcript&#44; and controls HAS2 <span class="elsevierStyleItalic">via</span> epigenetics&#46;<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">10</span></a> For instance&#44; a study<a class="elsevierStyleCrossRef" href="#bib0055"><span class="elsevierStyleSup">11</span></a> manifested that Sirtuin 1 represses nuclear translocation of NF-&#954;B and lncRNA HAS2-AS1 expression to decrease HAS2 expression&#46; HAS2-AS1 as a natural antisense RNA can generate heterodimer of HAS2 mRNA&#47;HAS2-AS1 to foster HAS2 expression&#46;<a class="elsevierStyleCrossRef" href="#bib0060"><span class="elsevierStyleSup">12</span></a> Besides&#44; HAS2-AS1 as a lncRNA is proved to impose an oncogenic role in multiple cancers including glioblastoma&#44;<a class="elsevierStyleCrossRef" href="#bib0065"><span class="elsevierStyleSup">13</span></a> oral squamous cell carcinoma&#44;<a class="elsevierStyleCrossRef" href="#bib0070"><span class="elsevierStyleSup">14</span></a> and epithelial ovarian cancer&#46;<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">15</span></a> A study<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">16</span></a> authenticated that transcription factor USF1 binds to lncRNA HAS2-AS1 promotor&#44; thereby facilitating invasive and migratory capabilities of glioma&#46; The other study also exhibited that transcription factor C&#47;EBP&#946; stimulates human fetal lung fibroblast-1 &#40;HFL-1&#41; cell migration&#44; proliferation&#44; and inflammation through activating lncRNA HAS2-AS1 in hypoxia&#46;<a class="elsevierStyleCrossRef" href="#bib0085"><span class="elsevierStyleSup">17</span></a> Nonetheless&#44; the specific role of lncRNA HAS2-AS1 in glioma is not clear&#46;</p><p id="par0020" class="elsevierStylePara elsevierViewall">Here&#44; we analyzed the expression level of HAS2-AS1 in 80 clinical samples&#44; constructed receiver operating characteristic &#40;ROC&#41; curves to evaluate the diagnostic and prognostic value of HAS2-AS1 in glioma&#44; and provided a new direction for the clinical treatment of glioma&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Bioinformatics analysis</span><p id="par0025" class="elsevierStylePara elsevierViewall">Gene Expression Quantification data and miRNA Expression Quantification data of TCGA&#95;LGG and TCGA&#95;GBM were downloaded from TCGA database &#40;<a href="https://portal.gdc.cancer.gov/">https&#58;&#47;&#47;portal&#46;gdc&#46;cancer&#46;gov&#47;</a>&#41;&#44; and glioma tissues along with normal tissues of 670 patients were obtained&#46; EdgeR was used for differential analysis with &#124;logFC&#124;<span class="elsevierStyleHsp" style=""></span>&#62;<span class="elsevierStyleHsp" style=""></span>2 and padj<span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05 as the threshold to find out the differentially expressed lncRNAs &#40;DElncRNAs&#41;&#44; and survival analysis was carried out on DElncRNAs&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Sample selection</span><p id="par0030" class="elsevierStylePara elsevierViewall">This study collected tumor and adjacent normal tissue samples from 80 patients with glioma admitted to Tangshan Gongren Hospital from January 2015 to January 2018&#46; All patients had not received any treatment before the operation&#46; The tissue samples of the patients were obtained during the operation and all specimens were stored in liquid nitrogen for rapid freezing after resection&#46; The tumor histology of each sample was assessed by senior pathologists&#46; The specimens were obtained with the approval and consent of the ethics committee of Tangshan Gongren Hospital&#44; and all patients had signed the informed consent&#46; The patients were classified into the WHO &#40;I&#8211;II&#41; grade and the WHO &#40;III&#8211;IV&#41; grade according to the WHO classification standard&#46; The pathological factors of the patients including gender&#44; age&#44; tumor size&#44; pathological classification&#44; tissue type and WHO grade were listed in <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">qRT-PCR</span><p id="par0035" class="elsevierStylePara elsevierViewall">Two &#956;g of total RNA was isolated from tissue samples or cultured cells using Trizol reagent &#40;Invitrogen&#44; Carlsbad&#44; California&#41; following manufacturer&#39;s instructions&#46; The isolated RNA was treated using PrimeScript RT Master Mix &#40;Takara&#44; Dalian&#44; China&#41; to prepare complementary DNA &#40;cDNA&#41; for PCR analysis&#46; PCR reaction was performed on LightCycler 480 system using TB GreenPremix Ex Taq II &#40;Takara&#41;&#46; The corresponding primers were as follows&#58; HAS2-AS1&#58; 5&#8242;-GACAGGACCTTGAAGACTGGG-3&#8242; &#40;forward&#41; and 5&#8242;-GGGATGGAGGTCAGCAACAA-3&#8242; &#40;reverse&#41;&#59; GAPDH&#58; 5&#8242;-ACAACTTTGGTATCGTGGAAGG-3&#8242; &#40;forward&#41; and 5&#8242;-GCCATCACGCCACA GTTTC-3&#8242; &#40;reverse&#41;&#46; GAPDH was the internal reference of HAS2-AS1&#46; HAS2-AS1 expression level was calculated by 2<span class="elsevierStyleSup">&#8722;&#916;&#916;Ct</span> method&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Statistical analysis</span><p id="par0040" class="elsevierStylePara elsevierViewall">GraphPad Prism &#40;version 7&#46;0&#44; GraphPad software&#44; Inc&#46;&#44; La Jolla&#44; CA&#44; USA&#41; and SPSS software &#40;version 23&#46;0&#44; SPSS Inc&#46;&#44; Chicago&#44; IL&#41; were used for data analysis&#46; Kaplan&#8211;Meier curve was used to analyze the overall survival &#40;OS&#41; of glioma patients&#46; Chi-square test was used to examine the correlation between HAS2-AS1 expression level and clinicopathological parameters&#46; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05 indicated a statistically significant difference&#46; The diagnostic ability of HAS2-AS1 in glioma was detected by ROC curve&#46; ROC curve was drawn by using SPSS 23&#46;0&#44; and the area under the curve &#40;AUC&#41; was calculated using rank-sum test&#46; The larger the area&#44; the higher the judgment value&#46; The &#8220;Youden index&#8221; was determined using the best cutoff value&#46; The maximum value of the index was the best boundary value&#46;</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0100">Follow-up visit</span><p id="par0045" class="elsevierStylePara elsevierViewall">80 patients were followed up at least once&#46; The follow-up was mainly to detect the expression of HAS2-AS1 in the patients and observe the prognostic effect&#46; The follow-up period began from the first day of treatment to May 31&#44; 2018&#46; A total of 80 patients were followed up for 6&#8211;36 months and the median follow-up time was 12&#46;3 months&#46; One patient was lost to follow-up&#44; with a total follow-up rate of 98&#46;75&#37;&#46; OS refers to the duration time from the first day after surgery to death or from the last follow-up time to the time of loss of follow-up&#46; Progression-free survival &#40;PFS&#41; is the period from the first day of surgery to recurrence and metastasis of patients&#46;</p></span></span><span id="sec0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0105">Results</span><span id="sec0045" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0110">Results of bioinformatics analysis</span><p id="par0050" class="elsevierStylePara elsevierViewall">EdgeR differential analysis results showed that a total of 557 DElncRNAs were obtained &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>A&#41;&#44; among which HAS2-AS1 was highly expressed in glioma &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>B&#41;&#46; Survival analysis indicated that high expression of HAS2-AS1 indicated an extremely high survival risk&#44; suggesting that HAS2-AS1 was of great survival significance &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>C&#41;&#46; Hence&#44; HAS2-AS1 was likely to be a potential diagnostic and prognostic marker for glioma&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia></span><span id="sec0050" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0115">HAS2-AS1 is up-regulated in glioma tissue with good diagnostic value</span><p id="par0055" class="elsevierStylePara elsevierViewall">The expression level of HAS2-AS1 in clinical glioma tissue and normal tissue of 80 patients was evaluated by qRT-PCR&#46; The results revealed that the relative mRNA expression of HAS2-AS1 in glioma tissues was significantly higher than that in normal tissues &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;0001&#44; <a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>A&#41;&#46; ROC curves were plotted based on HAS2-AS1 expression in glioma and normal tissues in the study&#46; It could be concluded from the ROC curves that AUC value was 0&#46;863 with the cut-off value of 5&#46;3&#44; sensitivity of 75&#37;&#44; specificity of 92&#46;5&#37; and Youden index of 67&#46;5&#37;&#46; HAS2-AS1 was confirmed to possess good sensitivity and specificity in the diagnosis of glioma&#44; along with good diagnostic value indicated by the AUC and Youden index&#46; Furthermore&#44; glioma tissues could be distinguished from normal tissues according to the expression level of HAS2-AS1 &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>B&#41;&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia></span><span id="sec0055" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0120">The relationship between HAS2-AS1 expression and the clinicopathological characteristics of glioma patients</span><p id="par0060" class="elsevierStylePara elsevierViewall">Here&#44; glioma patients were divided into high-expression group &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>40&#41; and low-expression group &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>40&#41; according to median HAS2-AS1 expression level&#44; and the correlation between HAS2-AS1 expression level and clinicopathological factors in glioma patients was detected by chi-square test&#46; There was no significant correlation between HAS2-AS1 expression level and age&#44; gender&#44; pathological classification&#44; and tissue type &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#62;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#41;&#46; While tumor size &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;002&#41; and WHO grade &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>0&#46;01&#41; were remarkably correlated with HAS2-AS1 expression &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46;</p></span><span id="sec0060" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0125">Tumor size and WHO grade are significantly correlated with HAS2-AS1 expression in glioma</span><p id="par0065" class="elsevierStylePara elsevierViewall">It had been concluded from <a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a> that tumor size and WHO grade were dramatically correlated with HAS2-AS1 expression level&#46; Box plots were used to demonstrate the specific correlation&#46; It could be observed that the expression level of HAS2-AS1 in glioma tissues with a tumor size of more than 4<span class="elsevierStyleHsp" style=""></span>cm was obviously higher than that in glioma tissues with a size of less than 4<span class="elsevierStyleHsp" style=""></span>cm &#40;<a class="elsevierStyleCrossRef" href="#fig0015">Fig&#46; 3</a>A&#41;&#46; Additionally&#44; the expression of HAS2-AS1 in WHO grade III&#8211;IV glioma tissues was also significantly higher than that in the I&#8211;II glioma tissues &#40;<a class="elsevierStyleCrossRef" href="#fig0015">Fig&#46; 3</a>B&#41;&#46;</p><elsevierMultimedia ident="fig0015"></elsevierMultimedia></span><span id="sec0065" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0130">High expression of HAS2-AS1 indicates poor prognosis and poor survival rate</span><p id="par0070" class="elsevierStylePara elsevierViewall">Survival analysis revealed that patients with low HAS2-AS1 expression share an relatively optimal survival &#40;<span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;01&#41;&#44; suggesting that high HAS2-AS1 expression was related to poor OS &#40;<a class="elsevierStyleCrossRef" href="#fig0020">Fig&#46; 4</a>A&#41;&#46; PFS curves exhibited that patients with low HAS2-AS1 expression had significantly higher PFS&#44; revealing that high expression of HAS2-AS1 suggested poor PFS &#40;<a class="elsevierStyleCrossRef" href="#fig0020">Fig&#46; 4</a>B&#41;&#46; ROC analysis for the prognostic value of HAS2-AS1 in OS of glioma patients showed the AUC value of 0&#46;906&#44; cut-off value of 5&#46;3&#44; sensitivity of 75&#37;&#44; specificity of 97&#46;5&#37;&#44; and Youden index of 72&#46;5&#37; &#40;<a class="elsevierStyleCrossRef" href="#fig0020">Fig&#46; 4</a>C&#41;&#46; ROC analysis for the prognostic value of HAS2-AS1 in PFS of glioma patients showed the AUC value of 0&#46;88&#44; cut-off value of 4&#46;9&#44; sensitivity of 95&#37;&#44; specificity of 85&#37;&#44; and Youden index of 65&#46;5&#37; &#40;<a class="elsevierStyleCrossRef" href="#fig0020">Fig&#46; 4</a>D&#41;&#46; The above data suggested that high expression of HAS2-AS1 was associated with poor prognosis in glioma patients and had good prognostic value&#46;</p><elsevierMultimedia ident="fig0020"></elsevierMultimedia></span></span><span id="sec0070" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0135">Conclusion</span><p id="par0075" class="elsevierStylePara elsevierViewall">Glioma is a disease in the central nervous system with the highest mortality and is the leading cause of brain diseases related deaths in children and adults&#46;<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">18</span></a> Its incidence increases with age and peaks between 75 and 84&#46;<a class="elsevierStyleCrossRef" href="#bib0095"><span class="elsevierStyleSup">19</span></a> Despite that most aggressive treatment methods have been applied for glioma&#44; the survival and prognosis remain poor&#44; with a median survival of 12&#8211;15 months and a low 5-year survival rate&#46;<a class="elsevierStyleCrossRefs" href="#bib0100"><span class="elsevierStyleSup">20&#44;21</span></a> The traditional treatment methods for glioma include surgical treatment&#44; radiotherapy and chemotherapy&#44; <span class="elsevierStyleItalic">etc&#46;</span> However&#44; due to the invasive growth of glioma&#44; the boundary of tumor tissues and adjacent normal brain tissues is unclear&#44; so it is difficult to completely resect the glioma tumor&#44; resulting in poor treatment and common recurrence&#46; Radiotherapy and chemotherapy are often used as adjuvant means after the surgery&#44; but they could not specifically kill tumor cells&#44; and may also produce toxic and side effects on the central nervous system&#44; so the treatment of glioma is one of the difficulties in neurosurgical research&#46;<a class="elsevierStyleCrossRefs" href="#bib0110"><span class="elsevierStyleSup">22&#8211;24</span></a> In order to change the clinical status that diagnosis and prognosis prediction of glioma disease can only depend on patient&#39;s age and tumor histological phenotypes&#44; a reliable biomarker is in need for clinical diagnosis of glioma and the prognosis prediction after surgical treatment&#46; LncRNAs can be applied in glioma and it has been reported that LINC00599 is down-regulated in glioma tissues and cell lines&#44; and is related to WHO grade and prognosis of glioma patients&#46;<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">25</span></a> Increased expression of lncRNA AB073614 may be a biomarker of poor prognosis in glioma&#46;<a class="elsevierStyleCrossRef" href="#bib0130"><span class="elsevierStyleSup">26</span></a> LncRNA MEG3 plays an important role in glioma cell proliferation&#44; apoptosis and autophagy&#44; and this gene is also significantly associated with the prognosis of glioma patients&#46;<a class="elsevierStyleCrossRef" href="#bib0135"><span class="elsevierStyleSup">27</span></a> All the above studies have proved that 1ncRNAs play a vital role in glioma&#46;</p><p id="par0080" class="elsevierStylePara elsevierViewall">Abnormal expression of the human hyaluronan synthase 2 &#40;HAS2&#41; gene has been linked to malignant tumors&#44; pulmonary arterial hypertension&#44; osteoarthritis&#44; asthma&#44; thyroid dysfunction&#44; and large organ fibrosis&#46;<a class="elsevierStyleCrossRef" href="#bib0140"><span class="elsevierStyleSup">28</span></a> Studies have indicated that HAS2-AS1 knockout can functionally inhibit the proliferation&#44; invasion and tumor growth of ovarian cancer cells <span class="elsevierStyleItalic">in vitro</span> and in <span class="elsevierStyleItalic">vivo</span>&#44; while overexpression of HAS2-AS1 indicates poor clinical prognosis of patients with ovarian cancer&#46;<a class="elsevierStyleCrossRef" href="#bib0075"><span class="elsevierStyleSup">15</span></a> It is found that the reduction of HAS2-AS1 inhibits the migration and invasion of glioma cells <span class="elsevierStyleItalic">in vivo</span> and <span class="elsevierStyleItalic">in vitro</span>&#46;<a class="elsevierStyleCrossRef" href="#bib0065"><span class="elsevierStyleSup">13</span></a> In addition&#44; many investigations reported that HAS2-AS1 is involved in the regulation mechanism of ceRNA network to modulate the proliferation and migration of tumor cells&#46;<a class="elsevierStyleCrossRefs" href="#bib0145"><span class="elsevierStyleSup">29&#44;30</span></a></p><p id="par0085" class="elsevierStylePara elsevierViewall">Glioma tissues and adjacent normal tissues from 80 patients were involved in the study&#46; The results exhibited that the expression of HAS2-AS1 in glioma tissues was significantly higher than that in normal tissues&#44; which was consistent with the bioinformatics analysis results of HAS2-AS1 and glioma&#46;<a class="elsevierStyleCrossRef" href="#bib0150"><span class="elsevierStyleSup">30</span></a> Moreover&#44; the tumor size and WHO grade of glioma were correlated with the expression of HAS2-AS1 in tumors&#44; which was consistent with the results of the review and meta-analysis of lncRNAs and glioma&#46;<a class="elsevierStyleCrossRef" href="#bib0155"><span class="elsevierStyleSup">31</span></a> The OS of patients with high HAS2-AS1 expression was remarkably lower than that of patients with low expression&#46; The ROC curves proved that HAS2-AS1 had better diagnostic and prognostic value in glioma&#46; The survival curves suggested that high expression of HAS2-AS1 was associated with poor survival&#46; However&#44; only 80 patients were included in this study&#44; and the sample size was small&#44; leading to the inaccuracy of HAS2-AS1 expression in glioma&#46; In addition&#44; isocitrate dehydrogenase &#40;IDH&#41; 1&#47;2 mutation is the golden standard for glioma diagnosis&#46; Nevertheless&#44; this study was limited by the absence of discussion about the relationship between HAS2-AS1 and IDH1&#47;2&#46; In future studies&#44; the sample size should be enlarged to determine a more accurate value for the diagnosis and prognosis of glioma&#46;</p><p id="par0090" class="elsevierStylePara elsevierViewall">In summary&#44; HAS2-HA1 can be used as a clinical biomarker for the diagnosis and prognosis prediction of glioma&#46;</p></span><span id="sec0075" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0140">Authors&#8217; contributions</span><p id="par0095" class="elsevierStylePara elsevierViewall">AW Y and X G mainly contributed to revising the article&#46; JS G&#44; XH G and AW Y contributed to data analysis&#46; J L&#44; YY Z and GM R contributed to drafting and modifying the article&#46; JT W and K Z contributed to final approval of the version to be published&#46; DC W agreed to be accountable for all aspects of the work&#46;</p></span><span id="sec0080" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0145">Ethics approval and consent to participate</span><p id="par0100" class="elsevierStylePara elsevierViewall">This study was conducted in accordance with the Helsinki Declaration II and was approved by the Research Ethics Committee of Tangshan Gongren Hospital&#46; Written informed consent was obtained from individual or guardian participants&#46; &#40;GRYY-LL-2019-66&#41;&#46;</p></span><span id="sec0085" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0150">Consent for publication</span><p id="par0110" class="elsevierStylePara elsevierViewall">All authors consent to submit the manuscript for publication&#46;</p></span><span id="sec0090" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0155">Availability of data and materials</span><p id="par0115" class="elsevierStylePara elsevierViewall">The data used to support the findings of this study are included within the article&#46; The data and materials in the current study are available from the corresponding author on reasonable request&#46;</p></span><span id="sec0095" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0160">Funding</span><p id="par0120" class="elsevierStylePara elsevierViewall">Not applicable&#46;</p></span><span id="sec0100" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0165">Conflict of interest</span><p id="par0125" class="elsevierStylePara elsevierViewall">The authors declare no conflicts of interest&#46;</p></span></span>"
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              "titulo" => "The relationship between HAS2-AS1 expression and the clinicopathological characteristics of glioma patients"
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              "titulo" => "Tumor size and WHO grade are significantly correlated with HAS2-AS1 expression in glioma"
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              "titulo" => "High expression of HAS2-AS1 indicates poor prognosis and poor survival rate"
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    "fechaRecibido" => "2021-02-07"
    "fechaAceptado" => "2021-06-11"
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            1 => "Glioma"
            2 => "Biomarker"
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          "titulo" => "Abbreviations"
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          "palabras" => array:5 [
            0 => "lncRNAs"
            1 => "ROC"
            2 => "OS"
            3 => "WHO"
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        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Background</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Glioma presents high incidence and poor prognosis&#44; and therefore more effective treatments are needed&#46; Studies have confirmed that long non-coding RNAs &#40;lncRNAs&#41; basically regulate various human diseases including glioma&#46; It has been theorized that HAS2-AS1 serves as an lncRNA to exert an oncogenic role in varying cancers&#46; This study aimed to assess the value of lncRNA HAS2-AS1 as a diagnostic and prognostic marker for glioma&#46;</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">The miRNA expression data and clinical data of glioma were downloaded from the TCGA database for differential analysis and survival analysis&#46; In addition&#44; pathological specimens and specimens of adjacent normal tissue from 80 patients with glioma were used to observe the expression of HAS2-AS1&#46; The receiver operating characteristic &#40;ROC&#41; curve was used to analyze the diagnostic ability and prognostic value of HAS2-AS1 in glioma&#46; Meanwhile&#44; a Kaplan&#8211;Meier survival curve was plotted to evaluate the survival of glioma patients with different HAS2-AS1 expression levels&#46;</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">HAS2-AS1 was significantly upregulated in glioma tissues compared with normal tissue&#46; The survival curves showed that overexpression of HAS2-AS1 was associated with poor overall survival &#40;OS&#41; and progression-free survival &#40;PFS&#41;&#46; Several clinicopathological factors of glioma patients&#44; including tumor size and WHO grade&#44; were significantly correlated with HAS2-AS1 expression in tissues&#46; The ROC curve showed an area under the curve &#40;AUC&#41; value of 0&#46;863&#44; indicating that HAS2-AS1 had good diagnostic value&#46; The ROC curve for the predicted OS showed an AUC of 0&#46;906&#44; while the ROC curve for predicted PFS showed an AUC of 0&#46;88&#46; Both suggested that overexpression of HAS2-AS1 was associated with poor prognosis&#46;</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusions</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Normal tissues could be clearly distinguished from glioma tissues based on HAS2-AS1 expression&#46; Moreover&#44; overexpression of HAS2-AS1 indicated poor prognosis in glioma patients&#46; Therefore&#44; HAS2-AS1 could be used as a diagnostic and prognostic marker for glioma&#46;</p></span>"
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        "titulo" => "Resumen"
        "resumen" => "<span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Introducci&#243;n</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">Los gliomas presentan una alta incidencia y un mal pron&#243;stico&#44; por lo que es necesario un tratamiento m&#225;s efectivo&#46; Algunos estudios han confirmado que los ARN no codificantes de cadena larga &#40;ARNncl&#41; regulan diferentes enfermedades&#44; entre las que se incluyen los gliomas&#46; Se ha postulado que HAS2-AS1 act&#250;a como un ARNncl&#44; con un efecto oncog&#233;nico en diferentes tipos de c&#225;ncer&#46; Este estudio tiene como objetivo analizar el valor del ARNncl HAS2-AS1 como marcador diagn&#243;stico y pron&#243;stico de glioma&#46;</p></span> <span id="abst0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">M&#233;todos</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">Descargamos los datos cl&#237;nicos y de expresi&#243;n de micro-ARN de la base de datos del Atlas del Genoma del C&#225;ncer &#40;TCGA&#41; para realizar el an&#225;lisis diferencial y de supervivencia&#46; Tambi&#233;n analizamos la expresi&#243;n de HAS2-AS1 en muestras patol&#243;gicas y muestras de tejido adyacente normal de 80 pacientes con glioma&#46; Para analizar la capacidad diagn&#243;stica y el valor pron&#243;stico de HAS2-AS1 en el glioma&#44; recurrimos a la curva ROC&#46; Tambi&#233;n utilizamos curvas de Kaplan-Meier para evaluar la supervivencia de los pacientes con glioma con diferentes niveles de expresi&#243;n de HAS2-AS1&#46;</p></span> <span id="abst0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Resultados</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">La expresi&#243;n de HAS2-AS1 era significativamente mayor en las muestras patol&#243;gicas que en el tejido normal&#46; Las curvas de supervivencia demostraron que la sobreexpresi&#243;n de HAS2-AS1 estaba relacionada con una menor supervivencia general y supervivencia libre de progresi&#243;n&#46; Algunos factores cl&#237;nico-patol&#243;gicos de los pacientes con glioma&#44; como el tama&#241;o del tumor y su grado&#44; seg&#250;n la clasificaci&#243;n de la OMS&#44; mostraron una correlaci&#243;n significativa con la expresi&#243;n de HAS2-AS1 en los tejidos afectados&#46; La curva ROC mostr&#243; un &#225;rea bajo la curva de 0&#44;863&#44; lo que indica que la expresi&#243;n de HAS2-AS1 posee un importante valor diagn&#243;stico&#46; El &#225;rea bajo la curva de la supervivencia general estimada fue de 0&#44;906&#59; para la supervivencia libre de progresi&#243;n estimada&#44; de 0&#44;88&#46; Ambos valores muestran que la sobreexpresi&#243;n de HAS2-AS1 se asocia con un mal pron&#243;stico&#46;</p></span> <span id="abst0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Conclusi&#243;n</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">Los tejidos normales pueden distinguirse claramente de los tejidos afectados por glioma en funci&#243;n de la expresi&#243;n de HAS2-AS1&#46; Adem&#225;s&#44; la sobreexpresi&#243;n de HAS2-HS1 fue indicativa de mal pron&#243;stico en los pacientes con glioma&#46; Por tanto&#44; HAS2-AS1 podr&#237;a utilizarse como marcador diagn&#243;stico y pron&#243;stico en el manejo del glioma&#46;</p></span>"
        "secciones" => array:4 [
          0 => array:2 [
            "identificador" => "abst0025"
            "titulo" => "Introducci&#243;n"
          ]
          1 => array:2 [
            "identificador" => "abst0030"
            "titulo" => "M&#233;todos"
          ]
          2 => array:2 [
            "identificador" => "abst0035"
            "titulo" => "Resultados"
          ]
          3 => array:2 [
            "identificador" => "abst0040"
            "titulo" => "Conclusi&#243;n"
          ]
        ]
      ]
    ]
    "multimedia" => array:5 [
      0 => array:7 [
        "identificador" => "fig0005"
        "etiqueta" => "Figure 1"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr1.jpeg"
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        "descripcion" => array:1 [
          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">TCGA expression profile analysis reveals that lncRNA HAS2-AS1 is significantly overexpressed in glioma and of great survival significance&#46; &#40;A&#41; The volcano map of edgeR differential analysis results&#59; &#40;B&#41; HAS2-AS1 expression level in glioma and normal tissues in TCGA&#59; &#40;C&#41; Survival rate of patients with high or low HAS2-AS1 expression were compared&#46;</p>"
        ]
      ]
      1 => array:7 [
        "identificador" => "fig0010"
        "etiqueta" => "Figure 2"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr2.jpeg"
            "Alto" => 1033
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            "Tamanyo" => 97355
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        "descripcion" => array:1 [
          "en" => "<p id="spar0050" class="elsevierStyleSimplePara elsevierViewall">Expression level of HAS2-AS1 in normal tissues and glioma tissues and its diagnostic value&#46; &#40;A&#41; The expression level of HAS2-AS1 in clinical normal tissues &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>80&#41; and glioma tissues &#40;<span class="elsevierStyleItalic">n</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>80&#41;&#59; &#40;B&#41; The ROC curves were plotted for evaluation of the diagnostic value of HAS2-AS1 expression in glioma &#40;the sensitivity is 75&#37; and the specificity is 92&#46;5&#37;&#41;&#46; AUC refers to area under the curve&#46; &#42;&#42;&#42;&#42; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;0001&#46;</p>"
        ]
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      2 => array:7 [
        "identificador" => "fig0015"
        "etiqueta" => "Figure 3"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr3.jpeg"
            "Alto" => 962
            "Ancho" => 2917
            "Tamanyo" => 86612
          ]
        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">Tumor size and WHO grade are significantly correlated with HAS2-AS1 expression&#46; Box plots were used to display the relationship between HAS2-AS1 expression and &#40;A&#41; tumor size or &#40;B&#41; WHO grade&#46; &#42;&#42;&#42;&#42; <span class="elsevierStyleItalic">P</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;0001&#46;</p>"
        ]
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      3 => array:7 [
        "identificador" => "fig0020"
        "etiqueta" => "Figure 4"
        "tipo" => "MULTIMEDIAFIGURA"
        "mostrarFloat" => true
        "mostrarDisplay" => false
        "figura" => array:1 [
          0 => array:4 [
            "imagen" => "gr4.jpeg"
            "Alto" => 1779
            "Ancho" => 2500
            "Tamanyo" => 119724
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        ]
        "descripcion" => array:1 [
          "en" => "<p id="spar0060" class="elsevierStyleSimplePara elsevierViewall">Effect of HAS2-AS1 expression on OS and PFS in glioma patients and corresponding prognostic value&#46; The &#40;A&#41; OS and &#40;B&#41; PFS of patients with high HAS2-AS1 expression were compared with those of patients with low expression&#59; the predictive value of HAS2-AS1 expression in &#40;C&#41; OS &#40;sensitivity of 75&#37;&#44; specificity of 97&#46;5&#37;&#41; and &#40;D&#41; PFS &#40;sensitivity of 95&#37;&#44; specificity of 85&#37;&#41; of glioma patients&#46;</p>"
        ]
      ]
      4 => array:8 [
        "identificador" => "tbl0005"
        "etiqueta" => "Table 1"
        "tipo" => "MULTIMEDIATABLA"
        "mostrarFloat" => true
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        "detalles" => array:1 [
          0 => array:3 [
            "identificador" => "at1"
            "detalle" => "Table "
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                  \t\t\t\t" scope="col">Characteristics&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t"><span class="elsevierStyleHsp" style=""></span>Female&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t  " align="char" valign="\n
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                  \t\t\t\t">15&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
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                  \t\t\t\t"><span class="elsevierStyleHsp" style=""></span>&#8807;4<span class="elsevierStyleHsp" style=""></span>cm&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t">42&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t">28&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t"><span class="elsevierStyleHsp" style=""></span>&#60;4<span class="elsevierStyleHsp" style=""></span>cm&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="\n
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                  \t\t\t\t">38&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td><td class="td" title="\n
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                  \t\t\t\t</td><td class="td" title="\n
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                  \t\t\t\t</td><td class="td" title="\n
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                  \t\t\t\t"><span class="elsevierStyleHsp" style=""></span>Mixed&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td><td class="td" title="\n
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                  \t\t\t\t</td><td class="td" title="\n
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                  \t\t\t\t">26&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t">60&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t">25&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
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                  \t\t\t\t"><span class="elsevierStyleHsp" style=""></span>III&#8211;IV&nbsp;\t\t\t\t\t\t\n
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                    0 => array:2 [
                      "titulo" => "The 2016 World Health Organization Classification of Tumors of the Central Nervous System&#58; a summary"
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                          "etal" => true
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                        "tituloSerie" => "Virchows Archiv &#58; an international journal of pathology&#46;"
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ISSN: 02134853
Original language: English
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