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Brief report
Protothecosis in a patient with T cell lymphocytic leukemia
Prototecosis en un paciente con leucemia linfocítica de células T
Mariana S. Fernándeza,
Corresponding author
mariana_f19@hotmail.com

Corresponding author.
, Florencia D. Rojasb, María E. Cattanaa, Javier E. Mussina, María de los Ángeles Sosab, Carlos D. Benzonic, Gustavo E. Giusianoa
a Universidad Nacional del Nordeste, CONICET, Instituto de Medicina Regional, Departamento de Micología, Resistencia, Chaco, Argentina
b Universidad Nacional del Nordeste, Instituto de Medicina Regional, Departamento de Micología, Resistencia, Chaco, Argentina
c Sanatorio Frangioli 2000, Servicio de Infectología, Resistencia, Chaco, Argentina
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    "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall">Human protothecosis is a rare infection caused by achlorophyllic algae of the genus <span class="elsevierStyleItalic">Prototheca</span>&#44; being <span class="elsevierStyleItalic">Prototheca wickerhamii</span> the species most commonly isolated<a class="elsevierStyleCrossRefs" href="#bib0105"><span class="elsevierStyleSup">8&#44;10&#44;11</span></a>&#46; These organisms are ubiquitous and mainly found in soil&#44; fresh and salty water&#44; slime flux of trees&#44; sewage&#44; animal waste&#44; as well as in some types of food&#46; Protothecosis have been classified into three clinical forms &#40;i&#41; cutaneous&#47;subcutaneous lesions&#44; &#40;ii&#41; olecranon bursitis&#44; and &#40;iii&#41; disseminated or systemic manifestations<a class="elsevierStyleCrossRefs" href="#bib0070"><span class="elsevierStyleSup">1&#44;6&#44;8&#44;11</span></a>&#46; It is believed that <span class="elsevierStyleItalic">Prototheca</span> species may infect humans through contact with potential sources or by traumatic inoculation of the algae in exposed areas<a class="elsevierStyleCrossRefs" href="#bib0085"><span class="elsevierStyleSup">4&#8211;6&#44;8&#44;10</span></a>&#46;</p><p id="par0010" class="elsevierStylePara elsevierViewall">Olecranon bursitis and localized cutaneous infections are more commonly developed in immunocompetent patients&#44; whereas dissemination and visceral compromise mainly affect severely immunocompromised patients with cellular deficiency<a class="elsevierStyleCrossRefs" href="#bib0105"><span class="elsevierStyleSup">8&#44;10</span></a>&#46; The most common clinical presentation is a vesiculobullous and ulcerative lesion with pustules and scabs&#44; simulating bacterial&#44; fungal or herpetic infections or eczema<a class="elsevierStyleCrossRef" href="#bib0085"><span class="elsevierStyleSup">4</span></a>&#46;</p><p id="par0015" class="elsevierStylePara elsevierViewall">We report a case of protothecosis caused by <span class="elsevierStyleItalic">P&#46; wickerhamii</span>&#46; For six years the patient developed diverse cold subcutaneous abscesses without an accurate diagnosis&#46; In this period&#44; she also presented a history of disseminated histoplasmosis and a diagnosis of chronic leukemia&#46;</p><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Case report</span><p id="par0020" class="elsevierStylePara elsevierViewall">A Caucasian 56-year-old woman&#44; born and living in Charata city&#44; located in the southwest of Chaco province &#40;Argentina&#41; attended our Mycology Department in November 2014 &#40;day 0&#41; with an abscess on her left ankle &#40;<a class="elsevierStyleCrossRef" href="#fig0005">Fig&#46; 1</a>&#41;&#46; In April 2009&#44; the patient noticed the first abscess in her right hand palm&#44; which disappeared without treatment&#46; In January 2010&#44; the patient is admitted to emergency for pneumonia and histoplasmosis diagnosis was confirmed&#46; The patient was treated during 18 months&#46; In February 2013&#44; a second abscess developed in her left elbow&#46; It was diagnosed as olecranon bursitis and an excision biopsy was performed&#46; Histopathology revealed an inflammatory granuloma with abscessed center&#46; Hematologic and biochemical laboratory findings revealed lymphocytosis and polyclonal hypergammaglobulinemia&#46; Immunophenotyping using flow cytometry in peripheral blood and immunohistochemical techniques in the bone marrow biopsy suggested T-cell large granular lymphocyte &#40;T-LGL&#41; leukemia&#46; In November 2013&#44; a third abscess developed in her left hand palm&#46; The abscess was surgically removed and histopathologically studied&#46; Histopathological results reported organisms compatible with <span class="elsevierStyleItalic">Paracoccidioides brasiliensis</span>&#44; not confirmed by microbiological or serological studies&#46;</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0025" class="elsevierStylePara elsevierViewall">At day 0&#44; the direct examination and Giemsa stain of the fourth abscess puncture revealed non-budding structures with morula-like appearance and sporangia &#40;thecas&#41;&#44; containing endospores&#44; suggestive of <span class="elsevierStyleItalic">Prototheca</span> species &#40;<a class="elsevierStyleCrossRef" href="#fig0010">Fig&#46; 2</a>&#41;&#46; The sample was inoculated onto Sabouraud&#39;s glucose agar &#40;SGA&#41; containing chloramphenicol and incubated at 37<span class="elsevierStyleHsp" style=""></span>&#176;C&#46; After 48<span class="elsevierStyleHsp" style=""></span>h&#44; cultures showed cream whitish yeast-like colonies &#40;<a class="elsevierStyleCrossRef" href="#fig0015">Fig&#46; 3</a>&#41;&#46; The agent was identified as <span class="elsevierStyleItalic">P&#46; wickerhamii</span> using the API 20 C AUX V3&#46;0 identification system &#40;Profile 7040040&#41; &#40;bioM&#233;rieux&#44; Marcy 64 l&#8217;Etoile&#44; France&#41;&#46; Antifungal susceptibility testing was determined using the broth microdilution method according to document M27-A3 CLSI<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">3</span></a>&#46; Minimal inhibitory concentrations obtained were&#58; fluconazole<span class="elsevierStyleHsp" style=""></span>&#62;<span class="elsevierStyleHsp" style=""></span>64<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;ml&#59; voriconazole 1<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;ml&#59; itraconazole 2<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;ml&#59; amphotericin B 0&#46;5<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;ml&#59; terbinafine<span class="elsevierStyleHsp" style=""></span>&#62;<span class="elsevierStyleHsp" style=""></span>16<span class="elsevierStyleHsp" style=""></span>&#956;g&#47;ml&#46; Treatment was started with amphotericin B deoxycholate 0&#46;7<span class="elsevierStyleHsp" style=""></span>mg&#47;kg&#47;day to complete 3<span class="elsevierStyleHsp" style=""></span>g&#46;</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia><elsevierMultimedia ident="fig0015"></elsevierMultimedia><p id="par0030" class="elsevierStylePara elsevierViewall">In April 2015&#44; the patient&#39;s condition worsened and was hospitalized in the intensive care unit&#44; where she died two days later of a possible hemophagocytic syndrome&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Discussion</span><p id="par0035" class="elsevierStylePara elsevierViewall">Protothecosis is a rare infection that is generally not suspected and its pathogenesis is largely unknown&#46; Protothecal infection occurs mainly in immunocompromised patients with alterations in innate or cellular immunity<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">12</span></a>&#46; Patients under steroid treatment&#44; with diabetes mellitus&#44; solid-tissue or hematologic malignancy &#40;acute myelogenous leukemia&#44; Hodgkin lymphoma&#44; and chronic lymphocytic leukemia&#41; are somehow at risk of protothecosis<a class="elsevierStyleCrossRefs" href="#bib0105"><span class="elsevierStyleSup">8&#44;11&#44;12</span></a>&#46; In this case&#44; T-LGL leukemia could have been the predisposing factor that favored histoplasmosis and protothecosis&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Prototheca</span> spp&#46; are noted on routine hematoxylin&#8211;eosin staining but are best visualized with periodic acid-Schiff and Gomori methenamine-silver histochemical stains<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">5</span></a>&#46; If endosporulation is found&#44; <span class="elsevierStyleItalic">Prototheca</span> is easily recognizable in tissue&#44; but if the morula is not present&#44; it may be confused by inexperienced personnel&#46; The lack of characteristic endospores causes <span class="elsevierStyleItalic">Prototheca</span> to resemble non-sporulating cells of <span class="elsevierStyleItalic">Blastomyces dermatitidis</span>&#44; <span class="elsevierStyleItalic">Cryptococcus</span> spp&#46;&#44; <span class="elsevierStyleItalic">Paracoccidioides</span> spp&#46;&#44; and some stages of <span class="elsevierStyleItalic">Coccidioides</span> spp&#46;&#44; <span class="elsevierStyleItalic">Pneumocystis jirovecii</span>&#44; <span class="elsevierStyleItalic">Rhinosporidium seeberi</span>&#46; Thus&#44; diagnosis of <span class="elsevierStyleItalic">Prototheca</span> species infection by histopathology can be difficult<a class="elsevierStyleCrossRefs" href="#bib0090"><span class="elsevierStyleSup">5&#44;6&#44;8&#44;10</span></a>&#46; In our case&#44; direct examination of the purulent collection allowed a fast diagnosis&#44; since asexual sporangia containing endospores were clearly and easily distinguished&#46; However&#44; in the biopsy of the second abscess&#44; <span class="elsevierStyleItalic">Prototheca</span> was not observed and in the third one&#44; <span class="elsevierStyleItalic">P&#46; brasiliensis</span> was reported in the histopathological study&#46; Although the diagnosis of protothecosis was confirmed in our Institute when the fourth abscess developed&#44; the patient spent five years through different health centers without an accurate diagnosis&#46; Protothecosis can be diagnosed from histopathological staining&#44; but microbiological studies are required for an accurate diagnosis&#46; In none of those opportunities the culture was made&#44; which delayed the diagnosis&#46;</p><p id="par0045" class="elsevierStylePara elsevierViewall">At present&#44; there are no available guidelines for the performance and interpretation of susceptibility testing for this genus&#46; Furthermore&#44; it is known that there is no direct correlation between <span class="elsevierStyleItalic">in vitro</span> activity and clinical response&#44; with the exception of a few cases<a class="elsevierStyleCrossRefs" href="#bib0100"><span class="elsevierStyleSup">7&#44;13</span></a>&#46; In the case reported&#44; the performance and breakpoint interpretations were based on CLSI document M27-A3 for <span class="elsevierStyleItalic">Candida</span><a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">3</span></a>&#46; In general&#44; <span class="elsevierStyleItalic">Prototheca</span> species are susceptible to amphotericin B and variable to azoles such as fluconazole&#44; itraconazole&#44; and voriconazole<a class="elsevierStyleCrossRefs" href="#bib0075"><span class="elsevierStyleSup">2&#44;7&#44;9</span></a>&#46; In this case&#44; the <span class="elsevierStyleItalic">P&#46; wickerhamii</span> isolate showed high MIC against most tested antifungals except for AMB&#46; Due to the patient&#39;s death&#44; the therapeutic response could not be evaluated&#46;</p><p id="par0050" class="elsevierStylePara elsevierViewall">Around 160 cases of protothecosis have been reported worldwide<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">12</span></a>&#46; To our knowledge&#44; this is the first reported clinical case of protothecosis in a patient suffering from chronic leukemia and with a history of histoplasmosis&#46;</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Ethical responsibilities</span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Protection of human and animal subjects</span><p id="par0055" class="elsevierStylePara elsevierViewall">The authors declare that no experiments were performed on humans or animals for this study&#46;</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Confidentiality of data</span><p id="par0060" class="elsevierStylePara elsevierViewall">The authors declare that they have followed the protocols of their work center on the publication of patient data&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Right to privacy and informed consent</span><p id="par0065" class="elsevierStylePara elsevierViewall">The authors declare that no patient data appear in this article&#46;</p></span></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0055">Conflict of interest</span><p id="par0070" class="elsevierStylePara elsevierViewall">The authors have no conflict of interest to declare&#46;</p></span></span>"
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        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Human protothecosis is a rare infection caused by algae of the genus <span class="elsevierStyleItalic">Prototheca&#46; Prototheca wickerhamii</span> has been recognized as the main species that causes infection in immunocompromised hosts with deficits in innate or cellular immunity&#46; We report a case of persisting subcutaneous protothecosis in a patient with T-cell large granular lymphocyte leukemia&#44; who also presented a history of disseminated histoplasmosis&#46;</p></span>"
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ISSN: 03257541
Original language: English
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