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Los valores de bootstrap (1.000 repeticiones) se muestran arriba y debajo de los nodos. Los genotipos y especies obtenidos de la base de datos <span class="elsevierStyleItalic">Genbank</span> incluyen los números de acceso (ACCN). Los aislamientos de este estudio están marcados con rombos negros rellenos (♦) (barra horizontal<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>0,05 sustituciones por posición de nucleótido).</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "María del C. Rojas, Marcelo Rodríguez Fermepín, Florencia Gracia Martínez, Sixto R. Costamagna" "autores" => array:4 [ 0 => array:2 [ "nombre" => "María del C." "apellidos" => "Rojas" ] 1 => array:2 [ "nombre" => "Marcelo" "apellidos" => "Rodríguez Fermepín" ] 2 => array:2 [ "nombre" => "Florencia" "apellidos" => "Gracia Martínez" ] 3 => array:2 [ "nombre" => "Sixto R." "apellidos" => "Costamagna" ] ] ] ] ] "idiomaDefecto" => "es" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0325754117300044?idApp=UINPBA00004N" "url" => "/03257541/0000004900000003/v1_201708180123/S0325754117300044/v1_201708180123/es/main.assets" ] "itemAnterior" => array:19 [ "pii" => "S0325754117300482" "issn" => "03257541" "doi" => "10.1016/j.ram.2016.11.006" "estado" => "S300" "fechaPublicacion" => "2017-07-01" "aid" => "180" "copyright" => "Asociación Argentina de Microbiología" "documento" => "article" "crossmark" => 1 "licencia" => "http://creativecommons.org/licenses/by-nc-nd/4.0/" "subdocumento" => "fla" "cita" => "Rev Argent Microbiol. 2017;49:216-23" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:2 [ "total" => 1276 "formatos" => array:3 [ "EPUB" => 42 "HTML" => 913 "PDF" => 321 ] ] "en" => array:13 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Original article</span>" "titulo" => "Prevalence of <span class="elsevierStyleItalic">Trypanosoma cruzi</span> infection in dogs and small mammals in Nuevo León, Mexico" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => array:2 [ 0 => "en" 1 => "es" ] "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "216" "paginaFinal" => "223" ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Prevalencia de la infección por <span class="elsevierStyleItalic">Trypanosoma cruzi</span> en perros y pequeños mamíferos de Nuevo León, México" ] ] "contieneResumen" => array:2 [ "en" => true "es" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0010" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 399 "Ancho" => 1600 "Tamanyo" => 106200 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0020" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleItalic">Trypanosoma cruzi</span> from wild mammals and dogs. (A) Giemsa stained blood smear from an opossum showing trypomastigotes of <span class="elsevierStyleItalic">Trypanosoma cruzi</span>. (B) Histopathological lesions with amastigote nests in the cardiac muscle of <span class="elsevierStyleItalic">T. cruzi</span> infected dogs (arrow indicates amastigote nests). (C) Amastigote forms (arrow) of <span class="elsevierStyleItalic">T. cruzi</span> observed in two mixed-breed puppies.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Lucio Galaviz-Silva, Roberto Mercado-Hernández, José J. Zárate-Ramos, Zinnia J. Molina-Garza" "autores" => array:4 [ 0 => array:2 [ "nombre" => "Lucio" "apellidos" => "Galaviz-Silva" ] 1 => array:2 [ "nombre" => "Roberto" "apellidos" => "Mercado-Hernández" ] 2 => array:2 [ "nombre" => "José J." "apellidos" => "Zárate-Ramos" ] 3 => array:2 [ "nombre" => "Zinnia J." "apellidos" => "Molina-Garza" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S0325754117300482?idApp=UINPBA00004N" "url" => "/03257541/0000004900000003/v1_201708180123/S0325754117300482/v1_201708180123/en/main.assets" ] "en" => array:20 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">Brief report</span>" "titulo" => "Protothecosis in a patient with T cell lymphocytic leukemia" "tieneTextoCompleto" => true "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "224" "paginaFinal" => "226" ] ] "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "Mariana S. Fernández, Florencia D. Rojas, María E. Cattana, Javier E. Mussin, María de los Ángeles Sosa, Carlos D. Benzoni, Gustavo E. Giusiano" "autores" => array:7 [ 0 => array:4 [ "nombre" => "Mariana S." "apellidos" => "Fernández" "email" => array:1 [ 0 => "mariana_f19@hotmail.com" ] "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] 1 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">*</span>" "identificador" => "cor0005" ] ] ] 1 => array:3 [ "nombre" => "Florencia D." "apellidos" => "Rojas" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] ] ] 2 => array:3 [ "nombre" => "María E." "apellidos" => "Cattana" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 3 => array:3 [ "nombre" => "Javier E." "apellidos" => "Mussin" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] 4 => array:3 [ "nombre" => "María" "apellidos" => "de los Ángeles Sosa" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">b</span>" "identificador" => "aff0010" ] ] ] 5 => array:3 [ "nombre" => "Carlos D." "apellidos" => "Benzoni" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">c</span>" "identificador" => "aff0015" ] ] ] 6 => array:3 [ "nombre" => "Gustavo E." "apellidos" => "Giusiano" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "aff0005" ] ] ] ] "afiliaciones" => array:3 [ 0 => array:3 [ "entidad" => "Universidad Nacional del Nordeste, CONICET, Instituto de Medicina Regional, Departamento de Micología, Resistencia, Chaco, Argentina" "etiqueta" => "a" "identificador" => "aff0005" ] 1 => array:3 [ "entidad" => "Universidad Nacional del Nordeste, Instituto de Medicina Regional, Departamento de Micología, Resistencia, Chaco, Argentina" "etiqueta" => "b" "identificador" => "aff0010" ] 2 => array:3 [ "entidad" => "Sanatorio Frangioli 2000, Servicio de Infectología, Resistencia, Chaco, Argentina" "etiqueta" => "c" "identificador" => "aff0015" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor0005" "etiqueta" => "⁎" "correspondencia" => "Corresponding author." ] ] ] ] "titulosAlternativos" => array:1 [ "es" => array:1 [ "titulo" => "Prototecosis en un paciente con leucemia linfocítica de células T" ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 1083 "Ancho" => 1625 "Tamanyo" => 148524 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Cold subcutaneous abscess in the left ankle.</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><p id="par0005" class="elsevierStylePara elsevierViewall">Human protothecosis is a rare infection caused by achlorophyllic algae of the genus <span class="elsevierStyleItalic">Prototheca</span>, being <span class="elsevierStyleItalic">Prototheca wickerhamii</span> the species most commonly isolated<a class="elsevierStyleCrossRefs" href="#bib0105"><span class="elsevierStyleSup">8,10,11</span></a>. These organisms are ubiquitous and mainly found in soil, fresh and salty water, slime flux of trees, sewage, animal waste, as well as in some types of food. Protothecosis have been classified into three clinical forms (i) cutaneous/subcutaneous lesions, (ii) olecranon bursitis, and (iii) disseminated or systemic manifestations<a class="elsevierStyleCrossRefs" href="#bib0070"><span class="elsevierStyleSup">1,6,8,11</span></a>. It is believed that <span class="elsevierStyleItalic">Prototheca</span> species may infect humans through contact with potential sources or by traumatic inoculation of the algae in exposed areas<a class="elsevierStyleCrossRefs" href="#bib0085"><span class="elsevierStyleSup">4–6,8,10</span></a>.</p><p id="par0010" class="elsevierStylePara elsevierViewall">Olecranon bursitis and localized cutaneous infections are more commonly developed in immunocompetent patients, whereas dissemination and visceral compromise mainly affect severely immunocompromised patients with cellular deficiency<a class="elsevierStyleCrossRefs" href="#bib0105"><span class="elsevierStyleSup">8,10</span></a>. The most common clinical presentation is a vesiculobullous and ulcerative lesion with pustules and scabs, simulating bacterial, fungal or herpetic infections or eczema<a class="elsevierStyleCrossRef" href="#bib0085"><span class="elsevierStyleSup">4</span></a>.</p><p id="par0015" class="elsevierStylePara elsevierViewall">We report a case of protothecosis caused by <span class="elsevierStyleItalic">P. wickerhamii</span>. For six years the patient developed diverse cold subcutaneous abscesses without an accurate diagnosis. In this period, she also presented a history of disseminated histoplasmosis and a diagnosis of chronic leukemia.</p><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Case report</span><p id="par0020" class="elsevierStylePara elsevierViewall">A Caucasian 56-year-old woman, born and living in Charata city, located in the southwest of Chaco province (Argentina) attended our Mycology Department in November 2014 (day 0) with an abscess on her left ankle (<a class="elsevierStyleCrossRef" href="#fig0005">Fig. 1</a>). In April 2009, the patient noticed the first abscess in her right hand palm, which disappeared without treatment. In January 2010, the patient is admitted to emergency for pneumonia and histoplasmosis diagnosis was confirmed. The patient was treated during 18 months. In February 2013, a second abscess developed in her left elbow. It was diagnosed as olecranon bursitis and an excision biopsy was performed. Histopathology revealed an inflammatory granuloma with abscessed center. Hematologic and biochemical laboratory findings revealed lymphocytosis and polyclonal hypergammaglobulinemia. Immunophenotyping using flow cytometry in peripheral blood and immunohistochemical techniques in the bone marrow biopsy suggested T-cell large granular lymphocyte (T-LGL) leukemia. In November 2013, a third abscess developed in her left hand palm. The abscess was surgically removed and histopathologically studied. Histopathological results reported organisms compatible with <span class="elsevierStyleItalic">Paracoccidioides brasiliensis</span>, not confirmed by microbiological or serological studies.</p><elsevierMultimedia ident="fig0005"></elsevierMultimedia><p id="par0025" class="elsevierStylePara elsevierViewall">At day 0, the direct examination and Giemsa stain of the fourth abscess puncture revealed non-budding structures with morula-like appearance and sporangia (thecas), containing endospores, suggestive of <span class="elsevierStyleItalic">Prototheca</span> species (<a class="elsevierStyleCrossRef" href="#fig0010">Fig. 2</a>). The sample was inoculated onto Sabouraud's glucose agar (SGA) containing chloramphenicol and incubated at 37<span class="elsevierStyleHsp" style=""></span>°C. After 48<span class="elsevierStyleHsp" style=""></span>h, cultures showed cream whitish yeast-like colonies (<a class="elsevierStyleCrossRef" href="#fig0015">Fig. 3</a>). The agent was identified as <span class="elsevierStyleItalic">P. wickerhamii</span> using the API 20 C AUX V3.0 identification system (Profile 7040040) (bioMérieux, Marcy 64 l’Etoile, France). Antifungal susceptibility testing was determined using the broth microdilution method according to document M27-A3 CLSI<a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">3</span></a>. Minimal inhibitory concentrations obtained were: fluconazole<span class="elsevierStyleHsp" style=""></span>><span class="elsevierStyleHsp" style=""></span>64<span class="elsevierStyleHsp" style=""></span>μg/ml; voriconazole 1<span class="elsevierStyleHsp" style=""></span>μg/ml; itraconazole 2<span class="elsevierStyleHsp" style=""></span>μg/ml; amphotericin B 0.5<span class="elsevierStyleHsp" style=""></span>μg/ml; terbinafine<span class="elsevierStyleHsp" style=""></span>><span class="elsevierStyleHsp" style=""></span>16<span class="elsevierStyleHsp" style=""></span>μg/ml. Treatment was started with amphotericin B deoxycholate 0.7<span class="elsevierStyleHsp" style=""></span>mg/kg/day to complete 3<span class="elsevierStyleHsp" style=""></span>g.</p><elsevierMultimedia ident="fig0010"></elsevierMultimedia><elsevierMultimedia ident="fig0015"></elsevierMultimedia><p id="par0030" class="elsevierStylePara elsevierViewall">In April 2015, the patient's condition worsened and was hospitalized in the intensive care unit, where she died two days later of a possible hemophagocytic syndrome.</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0030">Discussion</span><p id="par0035" class="elsevierStylePara elsevierViewall">Protothecosis is a rare infection that is generally not suspected and its pathogenesis is largely unknown. Protothecal infection occurs mainly in immunocompromised patients with alterations in innate or cellular immunity<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">12</span></a>. Patients under steroid treatment, with diabetes mellitus, solid-tissue or hematologic malignancy (acute myelogenous leukemia, Hodgkin lymphoma, and chronic lymphocytic leukemia) are somehow at risk of protothecosis<a class="elsevierStyleCrossRefs" href="#bib0105"><span class="elsevierStyleSup">8,11,12</span></a>. In this case, T-LGL leukemia could have been the predisposing factor that favored histoplasmosis and protothecosis.</p><p id="par0040" class="elsevierStylePara elsevierViewall"><span class="elsevierStyleItalic">Prototheca</span> spp. are noted on routine hematoxylin–eosin staining but are best visualized with periodic acid-Schiff and Gomori methenamine-silver histochemical stains<a class="elsevierStyleCrossRef" href="#bib0090"><span class="elsevierStyleSup">5</span></a>. If endosporulation is found, <span class="elsevierStyleItalic">Prototheca</span> is easily recognizable in tissue, but if the morula is not present, it may be confused by inexperienced personnel. The lack of characteristic endospores causes <span class="elsevierStyleItalic">Prototheca</span> to resemble non-sporulating cells of <span class="elsevierStyleItalic">Blastomyces dermatitidis</span>, <span class="elsevierStyleItalic">Cryptococcus</span> spp., <span class="elsevierStyleItalic">Paracoccidioides</span> spp., and some stages of <span class="elsevierStyleItalic">Coccidioides</span> spp., <span class="elsevierStyleItalic">Pneumocystis jirovecii</span>, <span class="elsevierStyleItalic">Rhinosporidium seeberi</span>. Thus, diagnosis of <span class="elsevierStyleItalic">Prototheca</span> species infection by histopathology can be difficult<a class="elsevierStyleCrossRefs" href="#bib0090"><span class="elsevierStyleSup">5,6,8,10</span></a>. In our case, direct examination of the purulent collection allowed a fast diagnosis, since asexual sporangia containing endospores were clearly and easily distinguished. However, in the biopsy of the second abscess, <span class="elsevierStyleItalic">Prototheca</span> was not observed and in the third one, <span class="elsevierStyleItalic">P. brasiliensis</span> was reported in the histopathological study. Although the diagnosis of protothecosis was confirmed in our Institute when the fourth abscess developed, the patient spent five years through different health centers without an accurate diagnosis. Protothecosis can be diagnosed from histopathological staining, but microbiological studies are required for an accurate diagnosis. In none of those opportunities the culture was made, which delayed the diagnosis.</p><p id="par0045" class="elsevierStylePara elsevierViewall">At present, there are no available guidelines for the performance and interpretation of susceptibility testing for this genus. Furthermore, it is known that there is no direct correlation between <span class="elsevierStyleItalic">in vitro</span> activity and clinical response, with the exception of a few cases<a class="elsevierStyleCrossRefs" href="#bib0100"><span class="elsevierStyleSup">7,13</span></a>. In the case reported, the performance and breakpoint interpretations were based on CLSI document M27-A3 for <span class="elsevierStyleItalic">Candida</span><a class="elsevierStyleCrossRef" href="#bib0080"><span class="elsevierStyleSup">3</span></a>. In general, <span class="elsevierStyleItalic">Prototheca</span> species are susceptible to amphotericin B and variable to azoles such as fluconazole, itraconazole, and voriconazole<a class="elsevierStyleCrossRefs" href="#bib0075"><span class="elsevierStyleSup">2,7,9</span></a>. In this case, the <span class="elsevierStyleItalic">P. wickerhamii</span> isolate showed high MIC against most tested antifungals except for AMB. Due to the patient's death, the therapeutic response could not be evaluated.</p><p id="par0050" class="elsevierStylePara elsevierViewall">Around 160 cases of protothecosis have been reported worldwide<a class="elsevierStyleCrossRef" href="#bib0125"><span class="elsevierStyleSup">12</span></a>. To our knowledge, this is the first reported clinical case of protothecosis in a patient suffering from chronic leukemia and with a history of histoplasmosis.</p></span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Ethical responsibilities</span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">Protection of human and animal subjects</span><p id="par0055" class="elsevierStylePara elsevierViewall">The authors declare that no experiments were performed on humans or animals for this study.</p></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Confidentiality of data</span><p id="par0060" class="elsevierStylePara elsevierViewall">The authors declare that they have followed the protocols of their work center on the publication of patient data.</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Right to privacy and informed consent</span><p id="par0065" class="elsevierStylePara elsevierViewall">The authors declare that no patient data appear in this article.</p></span></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0055">Conflict of interest</span><p id="par0070" class="elsevierStylePara elsevierViewall">The authors have no conflict of interest to declare.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:10 [ 0 => array:3 [ "identificador" => "xres883259" "titulo" => "Abstract" "secciones" => array:1 [ 0 => array:1 [ "identificador" => "abst0005" ] ] ] 1 => array:2 [ "identificador" => "xpalclavsec869960" "titulo" => "Keywords" ] 2 => array:3 [ "identificador" => "xres883260" "titulo" => "Resumen" "secciones" => array:1 [ 0 => array:1 [ "identificador" => "abst0010" ] ] ] 3 => array:2 [ "identificador" => "xpalclavsec869961" "titulo" => "Palabras clave" ] 4 => array:2 [ "identificador" => "sec0005" "titulo" => "Case report" ] 5 => array:2 [ "identificador" => "sec0010" "titulo" => "Discussion" ] 6 => array:3 [ "identificador" => "sec0015" "titulo" => "Ethical responsibilities" "secciones" => array:3 [ 0 => array:2 [ "identificador" => "sec0020" "titulo" => "Protection of human and animal subjects" ] 1 => array:2 [ "identificador" => "sec0025" "titulo" => "Confidentiality of data" ] 2 => array:2 [ "identificador" => "sec0030" "titulo" => "Right to privacy and informed consent" ] ] ] 7 => array:2 [ "identificador" => "sec0035" "titulo" => "Conflict of interest" ] 8 => array:2 [ "identificador" => "xack295009" "titulo" => "Acknowledgments" ] 9 => array:1 [ "titulo" => "References" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2016-11-16" "fechaAceptado" => "2017-02-24" "PalabrasClave" => array:2 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec869960" "palabras" => array:4 [ 0 => "<span class="elsevierStyleItalic">Prototheca wickerhamii</span>" 1 => "Cold subcutaneous abscesses" 2 => "Amphotericin B" 3 => "Antifungal susceptibility test" ] ] ] "es" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec869961" "palabras" => array:4 [ 0 => "<span class="elsevierStyleItalic">Prototheca wickerhamii</span>" 1 => "Abscesos subcutáneos fríos" 2 => "Anfotericina B" 3 => "Sensibilidad antifúngica" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "en" => array:2 [ "titulo" => "Abstract" "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Human protothecosis is a rare infection caused by algae of the genus <span class="elsevierStyleItalic">Prototheca. Prototheca wickerhamii</span> has been recognized as the main species that causes infection in immunocompromised hosts with deficits in innate or cellular immunity. We report a case of persisting subcutaneous protothecosis in a patient with T-cell large granular lymphocyte leukemia, who also presented a history of disseminated histoplasmosis.</p></span>" ] "es" => array:2 [ "titulo" => "Resumen" "resumen" => "<span id="abst0010" class="elsevierStyleSection elsevierViewall"><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">La prototecosis humana es una infección rara causada por algas del género <span class="elsevierStyleItalic">Prototheca</span>. <span class="elsevierStyleItalic">Prototheca wickerhamii</span> ha sido reconocida como la principal especie causante de infección en huéspedes inmunocomprometidos, con déficit de inmunidad innata o celular. Presentamos un caso de prototecosis subcutánea persistente en un paciente con leucemia linfocítica granular de células T, con antecedentes de histoplasmosis diseminada.</p></span>" ] ] "multimedia" => array:3 [ 0 => array:7 [ "identificador" => "fig0005" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 1083 "Ancho" => 1625 "Tamanyo" => 148524 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">Cold subcutaneous abscess in the left ankle.</p>" ] ] 1 => array:7 [ "identificador" => "fig0010" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 1174 "Ancho" => 1626 "Tamanyo" => 342230 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">Structures having morula-like appearance (sporangia) containing endospores (arrows), typical of <span class="elsevierStyleItalic">Prototheca</span> spp. on Giemsa stain (1000×). Scale bars<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleHsp" style=""></span>μm.</p>" ] ] 2 => array:7 [ "identificador" => "fig0015" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 1265 "Ancho" => 1625 "Tamanyo" => 251825 ] ] "descripcion" => array:1 [ "en" => "<p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">Theca (sporangia) of <span class="elsevierStyleItalic">Prototheca wickerhamii</span> (arrow) (Lactophenol cotton blue, 1000×) from culture at 48<span class="elsevierStyleHsp" style=""></span>h. Scale bars<span class="elsevierStyleHsp" style=""></span>=<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleHsp" style=""></span>μm.</p>" ] ] ] "bibliografia" => array:2 [ "titulo" => "References" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "bibs0005" "bibliografiaReferencia" => array:13 [ 0 => array:3 [ "identificador" => "bib0070" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Protothecosis y neumocistosis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => "R. 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