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Inicio Annals of Hepatology Effect of S-Nitrosoglutathione (GSNO) added to the University of Wisconsin Solut...
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Vol. 3. Núm. 3.
Páginas 113 (julio - septiembre 2004)
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Vol. 3. Núm. 3.
Páginas 113 (julio - septiembre 2004)
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Effect of S-Nitrosoglutathione (GSNO) added to the University of Wisconsin Solution (UW): Mast cell degranulation during normothermic reperfusion
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Alejandra B. Quintana1, Joaquín V. Rodríguez2, Henrique L. Lenzi3, Edgardo E. Guibert4,
Autor para correspondencia
eguibert@fbioyf.unr.edu.ar

Address for correspondence:
1 Morfología, Departamento de Ciencias Fisiológicas
2 Farmacología, Departamento de Ciencias Fisiológicas
3 Departamento de Patología, Fundação Oswaldo Cruz (FIOCRUZ), Río de Janeiro, Brasil
4 Biología Molecular, Departamento de Ciencias Biológicas, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Suipacha 531 (S2002LRK) Rosario, Argentina
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Morphology of portal mast cells in rat liver after cold preservation/normothermic reperfusion: Rat livers were cold preserved (0°C) during 48 h in UW solution with the addition of 500 μM GSNO to improve liver preservation. GSNO is a S-nitrosothiol, which releases the vasodilator Nitric Oxide that acts on hepatic microvascular system protecting the liver from preservation/reperfusion injuries. Apart from hepatocytes and non-parenchymal cells, the resident cells, such as mast cells, appear to be involved in the pathogenesis of these injuries. Any increment in oxygen-free radicals induces mast cell degranulation and these alterations promote granulocyte infiltration during graft reperfusion in vivo.

Rat liver slices were stained with Giemsa of Lennet. Mast cells (M) were observed granulated and incremented in number and size in portal spaces (PS) that showed interstitial edema (*), after normothermic reperfusion (Figure A).

Figure 1A.
(0.09MB).

Figure B showed a magnification of the area delimitated with the rectangle in Figure A. In this picture, interstitial edema (*) and red granules of secretion around mast cells (arrows) can be seen.

Figure 1B.
(0.05MB).

Damages on mast cells were avoided replacing the concentration of 500 μM GSNO for one of 100 μM.

Copyright © 2004. Fundación Clínica Médica Sur, A.C.
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