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Annals of Hepatology
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Inicio Annals of Hepatology O-20 MOLECULAR AND BIOLOGICAL CHARACTERIZATION OF HEPATITIS B VIRUS SUBGENOTYPE ...
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Vol. 28. Núm. S1.
Abstracts of the 2022 Annual Meeting of the ALEH
(marzo 2023)
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Vol. 28. Núm. S1.
Abstracts of the 2022 Annual Meeting of the ALEH
(marzo 2023)
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O-20 MOLECULAR AND BIOLOGICAL CHARACTERIZATION OF HEPATITIS B VIRUS SUBGENOTYPE F1b CLUSTERS: UNRAVELING ITS ROLE IN HEPATOCARCINOGENESIS
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María Mercedes Elizalde1, Laura Mojseijczuk2, Micaela Speroni1, Luciana Tadey2, Belén Bouzas3, Lilia Mammana3, Rodolfo Héctor Campos2, Diego Martín Flichman1
1 Institute for Biomedical Research on Retroviruses and AIDS (INBIRS), CONICET, Buenos Aires University, Buenos Aires, Argentina
2 Department of Microbiology, Immunology, Biotechnology and Genetics, Chair of Virology, University of Buenos Aires, Autonomous City of Buenos Aires, Argentina
3 Virology Unit, Infectious Diseases Hospital Francisco J. Muñíz, Autonomous City of Buenos Aires, Argentina
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Vol. 28. Núm S1

Abstracts of the 2022 Annual Meeting of the ALEH

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Introduction and Objectives

Hepatitis B virus subgenotype F1b infection has been associated with the early occurrence of hepatocellular carcinoma in chronically infected patients from Alaska and Peru. In Argentina, however, despite the high prevalence of subgenotype F1b infection, this relationship has not been described. This study aimed to unravel the observed differences in the progression of the infection, and an in-depth molecular and biological characterization of the subgenotype F1b was performed.

Materials and Methods

99 subgenotype F1b full-length sequences were obtained, and phylogeny was addressed by the maximum likelihood method. The replicative capacity of the subgenotype F1b clones was assessed by qPCR, Southern and Northern blot analysis. Antigen expression was detected by electrochemiluminescence and Western blot. The analysis of signaling pathways associated with hepatocarcinogenesis was assessed by RT-qPCR.

Results

Phylogenetic analysis of subgenotype F1b genomes revealed the existence of two highly supported clusters. One of the clusters, designated as gtF1b Basal included sequences mostly from Alaska, Peru, and Chile, while the other, called gtF1b Cosmopolitan, contained samples mainly from Argentina and Chile. The clusters were characterized by a differential signature pattern of eight nucleotides distributed throughout the genome. In vitro characterization of representative clones from each cluster revealed major differences in viral RNA levels, virion secretion, and antigen expression levels. Interestingly, differential regulation in the expression of genes associated with tumorigenesis was also identified.

Conclusions

This study provides new insights into the molecular and biological characteristics of the subgenotype F1b clusters and contributes to unraveling the different clinical outcomes of subgenotype F1b chronic infections.

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