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Annals of Hepatology
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Inicio Annals of Hepatology P- 32 HEPATITIS A AND E VIRUSES IN CÓRDOBA, ARGENTINA: WASTEWATER-BASED EPIDEMI...
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Vol. 28. Núm. S1.
Abstracts of the 2022 Annual Meeting of the ALEH
(marzo 2023)
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Vol. 28. Núm. S1.
Abstracts of the 2022 Annual Meeting of the ALEH
(marzo 2023)
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P- 32 HEPATITIS A AND E VIRUSES IN CÓRDOBA, ARGENTINA: WASTEWATER-BASED EPIDEMIOLOGY AS A SILENT SENTINEL OF THE TREND OF VIRUS CIRCULATION IN THE COMMUNITY
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Anabella Fantilli1,2, Guadalupe Di Cola1,2, Paola Sicilia3, Gonzalo Castro3, María De Los Ángeles Marinzalda4,5, Ariana Cachi4,5, Gustavo Ibarra6, Laura López7, Maria Gabriela Barbás8, Silvia Nates1, Gisela Masachessi1,2, Maria Belén Pisano1,2, Viviana Ré1,2
1 Dr. J. M. Vanella Institute of Virology, Faculty of Medical Sciences, National University of Córdoba, Córdoba, Argentina
2 National Council for Scientific and Technical Research (CONICET), CABA, Argentina
3 Central Laboratory Department, Ministry of Health of the Province of Córdoba, Córdoba, Argentina.
4 National Institute of Aeronautic and Space Medicine, FAA, Cordoba, Argentina
5 Faculty of the Air Force, National Defense University, Córdoba, Argentina.
6 Bajo Grande Municipal Sewage Effluent Treatment Plant-Laboratory of Physicochemical and Bacteriological Analysis, Edar Bajo Grande, Córdoba, Argentina
7 Epidemiology Area, Ministry of Health of the Province of Córdoba, Córdoba, Argentina
8 Secretariat of Prevention and Health Promotion, Ministry of Health of the Province of Córdoba, Córdoba, Argentina
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Vol. 28. Núm S1

Abstracts of the 2022 Annual Meeting of the ALEH

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Introduction and Objectives

Monitoring wastewater for traces of viruses allows effective surveillance of entire communities, including symptomatic and asymptomatic infected individuals, providing information on whether a specific pathogen is circulating in a population. Such is the case of hepatitis A and E viruses (HAV, HEV). This study aimed to detect HAV and HEV in wastewater samples from Córdoba, Argentina, to provide insights into their circulation dynamics.

Materials and Methods

Sewage samples were monthly and weekly collected from 2017 to 2020 and from 2020 to 2021, respectively, from 4 wastewater treatment plants located in different regions of Córdoba. Furthermore, sewage collectors of 7 neighborhoods in Córdoba city were weekly sampled during 2021. A standardized methodology was carried out for virus concentration using PEG6000 and NaCl. After automated nucleic acid extraction, HAV and HEV molecular detection were performed by TaqMan® Fast Applied Biosystems single-step multiplex RT-qPCR and specific RT-Nested PCR. Positive samples were sequenced.

Results

From a total of 575 samples analyzed, 16 were RNA-HAV+ (2.80%) and 17 RNA-HEV+ (2.96%). Eight and two sequences were obtained, respectively. The HAV+ specimens were genotype IA. The majority of them belonged to 2017-2018 and were genetically close to those reported in the clinical specimens from the same period when the HAV outbreak in men who have sex with men occurred in Córdoba. The HEV+ samples belonged to genotype 3, and HEV higher occurrence was in 2021, mainly in 2 neighborhoods from Córdoba city.

Conclusions

The results show HAV and HEV circulation in Córdoba, despite the low number of clinical cases reported, suggesting a continuous silent circulation of these viruses in the general population. Environmental surveillance of wastewater, together with clinical monitoring, are key tools to track the viral circulation trends over time in the population and to identify hotspots of virus excretion.

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