P-49 CONCENTRATION OF IL-12 AND CXCL-10 IN CHRONIC LIVER DISEASES

Introduction and Objectives

Chronic liver diseases are characterized by persistent inflammation related to high production of cytokines such as IL-12 and chemokine CXCL-10/IP-10 that attract activated Th1 lymphocytes that increase the production of IFN-g and TNF-a, perpetuating the inflammatory cascade. This study aimed to compare serum levels of IL-12 and CXCL-10 in different etiologies of liver disease.

Materials and Methods

A cross-sectional and multicenter study was carried out, including subjects with alcoholism according to criteria WHO, without (OH) and with liver injury (cirrhosis, CiOH) and (Alcoholic Hepatitis, HA); non-alcoholic fatty liver (NAFLD) and chronic Hepatitis C (CHC), diagnosed by clinical, biochemical data. They were compared with control subjects (CT). For determination of IL-12 and CXCL-10 with Multiplex®-MERCK©. Statistical analysis by SPSS V.22 using U de Mann Whitney, p<0.05; values expressed as mean ± standard error.

Results

Included 20 subjects with NAFLD, 78 CHC, 14 HA, 20 CiOH, 15 OH y 60 CT. IL-12 was found elevated in OH, HA, CHC vs. CT in OH vs. HCc y HGNA (p≤0.05). CXCL-10 was found elevated in CiOH, HA, and CHC vs. CT(p≤0.050).

Conclusions

The IL-12 showed elevated levels in subjects with alcohol consumption and CHC vs. CT that activates other cell types involved in inflammation. CXCL-10 is induced by IFN-γ and was found elevated in CiOH, HA and CHC, exerting their biological effects through CXCR3, including activation of peripheral immune cells and apoptosis. The ratio of IL-12/CXCL-10 in OH increased 4.6 times, ratifying the participation in chronic and continual inflammatory response by alcohol consumption. IL-12 and CXCL-10 have an important role in alcohol-induced liver disease, confirming their contribution to inflammation, being evident in CXCL-10 in advanced stages of the disease by stimulating and favoring the migration of immune cells to the damage sites.

Funding

This work was partially financed by CONACyT SALUD-2016-272579 and PAPIIT- UNAM TA200515.