Estadísticas

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      "titulo" => "Detección del ADN del virus del papiloma humano por PCR en mujeres de alto riesgo. Validación de un protocolo"
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          "titulo" => "Detection of the DNA of the human papiloma virus by PCR in women of high risk. Validation of a protocol"
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    "titulo" => "Utilización de la reacción en cadena de la polimerasa (PCR) para el diagnóstico de la infección por Helicobacter pylori en pacientes pediátricos"
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        "titulo" => "The use of polymerase chain reaction &#40;PCR&#41; in the diagnosis of infection by Helicobacter pylori in pediatric patients"
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        "resumen" => "<span class="elsevierStyleBold">Fundamento&#58;</span> Para diagnosticar la infecci&#243;n producida por Helicobacter pylori se pueden utilizar diferentes m&#233;todos directos o indirectos&#46; En este trabajo hemos utilizado un m&#233;todo molecular&#58; la reacci&#243;n en cadena de la polimerasa &#40;PCR&#41; con iniciadores espec&#237;ficos del gen ureA&#44; a partir de 43 muestras de biopsia g&#225;strica de pacientes pedi&#225;tricos con sintomatolog&#237;a digestiva y se han comparado los resultados con los obtenidos mediante histolog&#237;a y cultivo&#46; M&#233;todos&#58; La histolog&#237;a y el cultivo se realizaron a partir de la muestra de biopsia g&#225;strica&#44; obtenida mediante endoscopia digestiva&#44; siguiendo la metodolog&#237;a habitual&#46; La PCR se realiz&#243; con iniciadores espec&#237;ficos del gen ureA amplificando un fragmento de 411 pb&#44; y detectado mediante electroforesis en geles de agarosa&#46; <br/>Resultados&#58; De las 43 muestras de biopsia g&#225;strica estudiadas&#44; se obtuvo resultado positivo por cultivo en 21 &#40;48&#44;8&#37;&#41;&#44; por histolog&#237;a en 25 &#40;58&#44;1&#37;&#41; y por PCR en 29 &#40;67&#44;4&#37;&#41;&#46; En 30 biopsias &#40;69&#44;7&#37;&#41; los resultados de PCR concordaron con los obtenidos con los m&#233;todos considerados de referencia &#40;histolog&#237;a y&#47;o cultivo&#41;&#46; En 7 muestras &#40;16&#44;3&#37;&#41; la PCR era positiva mientras que el m&#233;todo de referencia era negativo&#44; y en 6 biopsias &#40;13&#44;9&#37;&#41; la PCR fue negativa siendo positiva la histolog&#237;a &#40;5 muestras&#41; o la histolog&#237;a y el cultivo &#40;una muestra&#41;&#46; <br/>Conclusiones&#58; Con la t&#233;cnica de PCR se obtuvo el porcentaje m&#225;s alto de positividad de los m&#233;todos estudiados&#46; La utilizaci&#243;n del mayor n&#250;mero posible de m&#233;todos de diagn&#243;stico para la infecci&#243;n por H&#46; pylori aumenta la posibilidad de conseguir un diagn&#243;stico positivo&#46;"
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        "resumen" => "<span class="elsevierStyleBold">Background&#58;</span> Several direct or non direct methods may be used to detect H&#46; pylori infection&#46; A molecular method&#44; PCR with ureA primers&#44; was used in this study in 43 gastric biopsies obtained from paediatric patients with digestive symptomathology and the results were compared with those obtained by histology and culture&#46; <br/>Methods&#58; Culture and histological stain were performed from gastric biopsies taken at routine endoscopy following standard methodology&#46; A PCR with specific primers for the ureA gene was applied and the 411 pb fragment detected by agarose gel electrophoresis&#46; <br/>Results&#58; From the 43 gastric biopsies&#44; 21 were positive by culture &#40;48&#44;8&#37;&#41;&#44; 25 by histology &#40;58&#44;1&#37;&#41; and 29 by PCR &#40;67&#44;4&#37;&#41;&#46; In 30 biopsies &#40;69&#44;7&#37;&#41; PCR results were in good agreement with the reference method &#40;histology and&#47;or culture&#41;&#46; In 7 biopsies the PCR was positive &#40;16&#44;3&#37;&#41;&#44; although the reference method was negative and in 6 &#40;13&#44;9&#37;&#41; the PCR was negative being positive the histology &#40;5 samples&#41; or histology and culture &#40;1 sample&#41;&#46; <br/> <span class="elsevierStyleBold">Conclusions&#46;</span>&#58; PCR shows the highest positive percentage among the methods used&#46; The use of as many methods as possible in the H&#46; pylori infection&#44; increases the diagnosis of the infection&#46;"
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