Estadísticas

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      "titulo" => "Caracterización de las mutaciones del gen rpoB en aislamientos clínicos de Mycobacterium tuberculosis resistentes a rifampicina"
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        "resumen" => "Objetivo&#58; Evaluar un protocolo para la detecci&#243;n del virus del papiloma humano &#40;VPH&#41; mediante la reacci&#243;n en cadena de la polimerasa &#40;PCR&#41;&#46; M&#233;todo&#58; Se estudiaron mediante dos protocolos de PCR en nuestro laboratorio y en otro de referencia c&#233;lulas exfoliativas del cuello uterino de 93 mujeres de alto riesgo para la infecci&#243;n por VPH&#46; Cada laboratorio trabaj&#243; sin conocer los resultados del otro&#46; <br/>Resultados&#58; La detecci&#243;n del ADN del VPH aument&#243; del 29&#44;8 al 50&#44;5&#37; tras modificar la cantidad de c&#233;lulas para la extracci&#243;n de ADN&#46; La comparaci&#243;n con el laboratorio de referencia result&#243; en un porcentaje de acuerdo del 99&#44;0&#37;&#46; La hibridaci&#243;n espec&#237;fica identific&#243; la siguiente distribuci&#243;n de genotipos de VPH&#58; VPH 16&#44; 29&#44;8&#37;&#59; VPH 33&#44; 17&#37;&#59; VPH 11&#47;6&#44; 12&#44;7&#37;&#59; VPH 18&#44; 12&#44;7&#37;&#59; VPH 31&#44; 23&#44;4&#37;&#59; VPH 39&#44; 3&#44;6&#37;&#59; y VPH 51&#44; 4&#44;3&#37;&#46; La comparaci&#243;n con el laboratorio de referencia fue indicativa de que las sondas utilizadas subestiman la prevalencia de VPH 51&#46; El 100&#37; de las mujeres con lesiones displ&#225;sicas fueron VPH positivas&#44; confirmando la validez del protocolo&#46; <br/>Conclusiones&#58; La sensibilidad de la PCR en la detecci&#243;n de ADN del VPH puede incrementarse con m&#237;nimas modificaciones de protocolos est&#225;ndar&#46; Es por ello importante realizar estudios de validaci&#243;n constante para asegurar y mantener buenos niveles de sensibilidad&#46;"
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