Caracterización de Perfiles Proteicos y Peptídicos en Periodontitis Crónica

Hernández, M.; Obregón, F.; Pozo, P.; Barriga, A.; Valenzuela, M.A.

doi:10.1016/S0718-5391(08)70008-9

Información del artículo

Resumen

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Estadísticas

Resumen

La destrucción de los tejidos de soporte durante la periodontitis crónica está mediada por proteasas específicas. Producto de la actividad proteolítica se generarán cambios en la composición peptídica y proteica globales del fluido crevicular gingival (FCG). El objetivo de este estudio es evaluar la existencia de patrones de proteicos y peptídicos característicos en el FCG de pacientes con periodontitis crónica y sanos.

Materiales y métodos

Se seleccionaron 8 pacientes con periodontitis crónica moderada a severa y 5 sujetos controles y se obtuvieron muestras de FCG. Las muestras se eluyeron en 2 tampones, y se prepararon en 3 matrices distintas, y se obtuvieron los espectros peptídicos y proteicos mediante espectrometría de masas MALDI-TOF. Se procesaron 3 espectros de cada grupo para la obtención final de espectros diferenciales en enfermos.

Resultados

Se obtuvieron patrones proteicos y peptídicos reproducibles en el FCG. En pacientes con periodontitis crónica encontramos perfiles característicos representados por más de 100 proteínas que no fueron detectables en sujetos sanos.

Conclusiones

Demostramos la utilidad de la aplicación de la espectrometría de masas MALDI-TOF en el análisis de cambios cualitativos en la composición global del FCG y presentamos los perfiles proteicos y peptídicos característicos del FCG en periodontitis crónica y sujetos sanos.

Palabras claves:

Periodontitis crónica

FCG

MALDI-TOF

perfiles proteicos

Abstract

Inflammatory response mediates periodontal tissue destruction occurring in chronic periodontitis. As result, qualitative changes in global peptide and protein GCF composition will generate.

Aim

To evaluate existence of characteristic peptide and protein profiles in gingival crevicular fluid (GCF) from patients with chronic periodontitis and controls.

Methods

Patients with moderate to severe chronic periodontitis (n=8) and healthy controls (n=5) were selected and samples of GCF were obtained. Samples were eluted in 2 different buffers and prepared with 3 matrices to obtain peptide and protein spectra by MALDI-TOF mass spectrometry analysis. Subsequently, 3 spectra from each group were processed to obtain final differential spectra.

Results

Reproducible peptide and protein profiles were obtained from both stages in GCF. In disease samples we found characteristic patterns represented by more than 100 proteins which were undetectable in controls.

Conclusions

We demonstrate for the first time the usefulness of MALDI-TOF mass spectrometry in the analysis of qualitative global changes in GCF composition and we present protein and peptide characteristic GCF profiles associated with chronic periodontitis and healthy periodontium.

Key words:

Chronic periodontitis

GCF

MALDI-TOF

protein profiles

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