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Inicio Revista Clínica de Periodoncia, Implantología y Rehabilitación Oral Participación de MT1-MMP en la Remodelación del Ligamento Periodontal Durante ...
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Vol. 3. Núm. 3.
Páginas 113-117 (diciembre 2010)
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Vol. 3. Núm. 3.
Páginas 113-117 (diciembre 2010)
Open Access
Participación de MT1-MMP en la Remodelación del Ligamento Periodontal Durante la Movilización Dentaria
Role of MT1-MMP in the Remodeling of the Periodontal Ligament During Tooth Movement
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2719
P. Rey Droghetti1, F. Cruzat2, P. Smith Ferrer3,
Autor para correspondencia
psmith@med.puc.cl

Correspondencia autor: Laboratorio de Fisiología Periodontal, Carrera de Odontología. Facultad de Medicina, Pontificia Universidad Católica de Chile. Marcoleta 391, Santiago. Chile.
, A. Oyarzún Droguett4
1 Cirujano Dentista. Integramédica. Estudiante Postítulo en Periodoncia, Universidad Mayor. Chile
2 Cirujano Dentista. Centro Médico San Joaquín. Pontificia Universidad Católica de Chile. Chile
3 Carrera de Odontología. Facultad de Medicina, Pontificia Universidad Católica de Chile. Chile
4 Facultad de Odontología. Universidad Finis Terrae. Chile
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Resumen

La movilización dentaria involucra una serie de cambios en los tejidos de soporte caracterizados por la activa remodelación de estos. La MT1-MMP o MMP-14 es una potente enzima proteolítica capaz de degradar colágeno tipo I, la principal molécula estructural del ligamento periodontal. La migración dentaria requiere de la degradación controlada del colágeno constituyente del ligamento periodontal. Sin embargo, no existen evidencias de la participación de MT1-MMP en la remodelación del tejido periodontal durante este proceso. En el presente estudio hemos analizado la expresión de MT1-MMP y del marcador de actividad osteoclástica Fosfatasa Ácida Tartrato Resistente (TRAP) en un modelo de migración dentaria en ratas. La migración dentaria fue activada mediante la inserción de una banda separadora entre los incisivos superiores. La expresión y distribución de TRAP y MT1-MMP fue evaluada a través de citoquímica e inmunohistoquímica a los días 1, 3, 5 y 7. La producción de TRAP fue identificada principalmente en osteoclastos ubicados en la zona de compresión del ligamento periodontal. La producción de MT1-MMP fue observada en fibroblastos de la zona de compresión del ligamento periodontal y osteoclastos ubicados en esta misma región. Nuestros resultados permiten proponer que tanto MT1-MMP como TRAP participan en la remodelación de los tejidos de soporte periodontal durante la migración dentaria.

Palabras clave:
MT1-MMP
remodelación tisular
ligamento periodontal
osteoclasto
movimiento dentario
Abstract

Tooth movement involves a series of changes of the supporting periodontal tissues characterized by the active connective tissue remodeling. MT1- MMP or MMP-14 belongs to the family of matrix metalloproteinases that are able to degrade type I collagen, the main molecule involved in periodontal attachment. Tooth migration requires the controlled degradation of periodontal ligament collagen fibers. However, evidences linking MT1-MMP expression with periodontal tissue remodeling are lacking. In the present study, we have evaluated the expression of MT1-MMP and of the osteoclast marker Tartrate Resistant Acid Phosphatase (TRAP) in a model of tooth migration in rats. Tooth migration was induced after the insertion of a rubber band between the upper incisors. The distribution of TRAP and MT1-MMP was evaluated by means of cytochemistry and immunohistochemistry respectively at days 1, 3, 5 and 7. TRAP production was identified in osteoclasts at the area of compression of the periodontal ligament. MT1-MMP distribution was observed in fibroblasts at the compressed area of the periodontal ligament and also in osteoclasts of the same region. Our findings allow us to propose that MT1-MMP and TRAP take part of the tissue remodeling events observed during tooth movement.

Key words:
MT1-MMP
tissue remodeling
periodontal ligament
osteoclast
tooth movement
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Copyright © 2010. Sociedad de Periodoncia de Chile, Sociedad de Implantología Oral de Chile y Sociedad de Prótesis y Rehabilitación Oral de Chile
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