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Objetivo: Determinar las manifestaciones de lupus eritematoso sistémico (LES) relacionadas con los anticuerpos anti-U1-RNP no precipitantes en geles de agarosa.
Pacientes y métodos: Hemos utilizado técnicas de ELISA y contrainmunoelectroforesis (CIE) para detectar anticuerpos anti-U1-RNP en sueros de 154 pacientes diagnosticados de LES. Se consideran anticuerpos no precipitantes los detectados por ELISA que no precipitan en geles de agarosa (CIE negativa).
Resultados: Se detectan anticuerpos anti-U1-RNP precipitantes (ELISA y CIE positivos) en 28 pacientes (18,1%), todos de clase IgG, y anticuerpos anti-U1-RNP no precipitantes (ELISA positivo y CIE negativo) en 69 (44,8%), aunque sólo en 38 (20,1%) son de clase IgG. Los anticuerpos anti-U1-RNP precipitantes se asocian con edema en las manos, fenómeno de Raynaud, miositis y pericarditis. Los pacientes con anticuerpos anti-U1-RNP no precipitantes presentan una frecuencia significativamente menor de pericarditis, nefropatía e insuficiencia renal crónica. En particular, los pacientes con anticuerpos anti-U1-RNP no precipitantes presentan menos manifestaciones cardíacas y renales que los pacientes con anticuerpos precipitantes.
Conclusiones: El significado clínico de los anticuerpos anti-U1-RNP en el LES es diferente según precipiten o no en geles de agarosa. Los pacientes con anticuerpos anti-U1-RNP no precipitantes presentan una enfermedad moderada, con una menor frecuencia de nefropatía grave.
Pacientes y métodos: Hemos utilizado técnicas de ELISA y contrainmunoelectroforesis (CIE) para detectar anticuerpos anti-U1-RNP en sueros de 154 pacientes diagnosticados de LES. Se consideran anticuerpos no precipitantes los detectados por ELISA que no precipitan en geles de agarosa (CIE negativa).
Resultados: Se detectan anticuerpos anti-U1-RNP precipitantes (ELISA y CIE positivos) en 28 pacientes (18,1%), todos de clase IgG, y anticuerpos anti-U1-RNP no precipitantes (ELISA positivo y CIE negativo) en 69 (44,8%), aunque sólo en 38 (20,1%) son de clase IgG. Los anticuerpos anti-U1-RNP precipitantes se asocian con edema en las manos, fenómeno de Raynaud, miositis y pericarditis. Los pacientes con anticuerpos anti-U1-RNP no precipitantes presentan una frecuencia significativamente menor de pericarditis, nefropatía e insuficiencia renal crónica. En particular, los pacientes con anticuerpos anti-U1-RNP no precipitantes presentan menos manifestaciones cardíacas y renales que los pacientes con anticuerpos precipitantes.
Conclusiones: El significado clínico de los anticuerpos anti-U1-RNP en el LES es diferente según precipiten o no en geles de agarosa. Los pacientes con anticuerpos anti-U1-RNP no precipitantes presentan una enfermedad moderada, con una menor frecuencia de nefropatía grave.
Palabras clave:
Lupus eritematoso sistémico
Contrainmunoelectroforesis
ELISA
Anticuerpos anti-snRNP
Anticuerpos anti-U1-RNP
Objective: To determine the clinical features associated with non-precipitating anti-U1-RNP antibodies on agarose gels in patients with systemic lupus erythematosus (SLE).
Patients and methods: Anti-U1-RNP antibodies were detected by ELISA and by counterimmunoelectrophoresis (CIE) in sera samples of 154 patients with SLE. Non-precipitating antibodies were defined as antibodies detected by ELISA without precipitation on agarose gels.
Results: Precipitating anti-U1-RNP antibodies (positive ELISA and CIE) were detected in 28 patients (18.1%), all of them were of IgG class. Non-precipitating anti-U1-RNP antibodies (positive ELISA and negative CIE) were detected in 69 patients (44.8%), but only in 38 (20.1%) these antibodies were of IgG class. The presence of precipitating anti-U1-RNP antibodies was associated with swollen hands, Raynaud's phenomenon, myositis and pericarditis. Renal, disease, chronic renal failure and pericarditis were significantly less common in patients with non-precipitating anti-U1-RNP antibodies. Particularly, patients with non-precipitating anti-U1-RNP antibodies had less cardiac and renal manifestations than patients with precipitating anti-U1-RNP antibodies.
Conclusions: SLE patients with non-precipitating anti-U1-RNP antibodies have different clinical findings than patients with precipitating anti-U1-RNP antibodies or patients without these antibodies. SLE patients with non-precipitating anti-U1-RNP antibodies may represent a subset of patients with mild disease, with less severe renal manifestations.
Patients and methods: Anti-U1-RNP antibodies were detected by ELISA and by counterimmunoelectrophoresis (CIE) in sera samples of 154 patients with SLE. Non-precipitating antibodies were defined as antibodies detected by ELISA without precipitation on agarose gels.
Results: Precipitating anti-U1-RNP antibodies (positive ELISA and CIE) were detected in 28 patients (18.1%), all of them were of IgG class. Non-precipitating anti-U1-RNP antibodies (positive ELISA and negative CIE) were detected in 69 patients (44.8%), but only in 38 (20.1%) these antibodies were of IgG class. The presence of precipitating anti-U1-RNP antibodies was associated with swollen hands, Raynaud's phenomenon, myositis and pericarditis. Renal, disease, chronic renal failure and pericarditis were significantly less common in patients with non-precipitating anti-U1-RNP antibodies. Particularly, patients with non-precipitating anti-U1-RNP antibodies had less cardiac and renal manifestations than patients with precipitating anti-U1-RNP antibodies.
Conclusions: SLE patients with non-precipitating anti-U1-RNP antibodies have different clinical findings than patients with precipitating anti-U1-RNP antibodies or patients without these antibodies. SLE patients with non-precipitating anti-U1-RNP antibodies may represent a subset of patients with mild disease, with less severe renal manifestations.
Keywords:
Systemic lupus erythematosus
Counterimmunoelectrophoresis
ELISA
Anti-snRNP antibodies
Anti-U1-RNP antibodies