Association of IFNL3 gene rs4803217 with spontaneous clearance of Hepatitis C virus in patients from West Mexico

Ochoa-Cervantes, Andrea Y.; Torres-Reyes, Luis A.; Panduro, Arturo; Roman, Sonia; Gonzalez-Aldaco, Karina

doi:10.1016/j.aohep.2024.101393

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The identity of the patients is protected. Consentment was obtained.Declaration of interestsNoneFundingPrograma para el Desarrollo Profesional Docente supported this work toGonzalez-Aldaco K, No. UDG-PTC-1422.IGF" "pdfFichero" => "main.pdf" "tienePdf" => true "tieneResumen" => true "resumen" => array:1 [ "en" => array:2 [ "resumen" => "Introduction and ObjectivesCytokines are crucial in modulating immune responses and the outcome of hepatitis C virus (HCV) infection. Polymorphisms in the IL10 and IFNL3 genes have been associated with spontaneous clearance (SC) in populations worldwide. However, their effect on the Mexican population remains unknown. This study aimed to analyze the association of polymorphisms in the IL10 (rs1800871, rs1800872, and rs1800896) and IFNL3 (rs4803217) genes with SC in HCV patients from West Mexico. Materials and PatientsA total of 203 treatment-naïve, anti-HCV-positive patients were included in the study. Among them, 60 had undetectable viral load, and 143 had a detectable viral load. Genotyping was performed using Real-Time PCR. Biochemical and serological analyses were conducted. Comparative statistical analysis was performed using SPSSv24 software. Written informed consent was obtained from all participants. The Institutional Review Board approved this study. ResultsThe CC genotype of the IFNL3 rs4803217 gene was associated with SC (OR=2.046, p=0.026). In SC patients, the CC genotype increased total cholesterol (TChol) compared to AA+AC genotypes (209.76 vs. 176.86 mg/dL, p=0.061). The IL10 rs1800871, rs1800872, and rs1800896 polymorphisms were not associated with SC. In SC patients, the CC+CT genotypes of IL10 rs1800871 and CC+CA genotypes of IL10 rs1800872 were associated with higher TChol levels compared to the TT genotype (198.68 vs. 177.85 mg/dL, p=0.010) and the AA genotype (196.81 vs. 178.58 mg/dL, p=0.006), respectively. In chronic patients, the GG+GA genotypes of IL10 rs1800896 were associated with high insulin levels compared to the AA genotype (17.22 vs. 12.04 IU/mL, p=0.021). ConclusionsThe CC genotype of the IFNL3 rs4803217 gene was associated with SC in patients from West Mexico. IL10 and IFNL3 polymorphisms increased TChol in SC patients. These results suggest an interaction between metabolic and immune factors in the outcome of HCV infection." 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ISSN: 1665-2681

Annals of Hepatology (AoH) is an international, open access journal published bi-monthly with funds from the Fundación Clínica Médica Sur. It is the official journal of the Mexican Association of Hepatology (AMH), the Latin American Association for the Study of the Liver (ALEH), the Canadian Association for the Study of the Liver (CASL) and the Czech Society of Hepatology (CSH). AoH publishes editorials, opinions, concise reviews, original articles, brief reports, letters to the editor, news from affiliated associations, clinical practice guidelines and summaries of congresses in the field of Hepatology.

Topics covered by AoH include alcoholic liver disease, autoimmune hepatitis, biliary diseases, drug-induced liver injury, genetic liver diseases, NAFLD/NASH and viral hepatitis (HAV, HBV, HCV, HDV, HEV). Our journal seeks to publish articles on basic clinical care and translational research focused on preventing rather than treating the complications of end-stage liver disease.

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Cytokines are crucial in modulating immune responses and the outcome of hepatitis C virus (HCV) infection. Polymorphisms in the IL10 and IFNL3 genes have been associated with spontaneous clearance (SC) in populations worldwide. However, their effect on the Mexican population remains unknown. This study aimed to analyze the association of polymorphisms in the IL10 (rs1800871, rs1800872, and rs1800896) and IFNL3 (rs4803217) genes with SC in HCV patients from West Mexico.

Materials and Patients

A total of 203 treatment-naïve, anti-HCV-positive patients were included in the study. Among them, 60 had undetectable viral load, and 143 had a detectable viral load. Genotyping was performed using Real-Time PCR. Biochemical and serological analyses were conducted. Comparative statistical analysis was performed using SPSSv24 software. Written informed consent was obtained from all participants. The Institutional Review Board approved this study.

Results

The CC genotype of the IFNL3 rs4803217 gene was associated with SC (OR=2.046, p=0.026). In SC patients, the CC genotype increased total cholesterol (TChol) compared to AA+AC genotypes (209.76 vs. 176.86 mg/dL, p=0.061). The IL10 rs1800871, rs1800872, and rs1800896 polymorphisms were not associated with SC. In SC patients, the CC+CT genotypes of IL10 rs1800871 and CC+CA genotypes of IL10 rs1800872 were associated with higher TChol levels compared to the TT genotype (198.68 vs. 177.85 mg/dL, p=0.010) and the AA genotype (196.81 vs. 178.58 mg/dL, p=0.006), respectively. In chronic patients, the GG+GA genotypes of IL10 rs1800896 were associated with high insulin levels compared to the AA genotype (17.22 vs. 12.04 IU/mL, p=0.021).

Conclusions

The CC genotype of the IFNL3 rs4803217 gene was associated with SC in patients from West Mexico. IL10 and IFNL3 polymorphisms increased TChol in SC patients. These results suggest an interaction between metabolic and immune factors in the outcome of HCV infection.

Full Text

Ethical statement

The protocol was registered and approved by the Ethics Committee. The identity of the patients is protected. Consentment was obtained.

Declaration of interests

None

Funding

Programa para el Desarrollo Profesional Docente supported this work to

Gonzalez-Aldaco K, No. UDG-PTC-1422.IGF