ORIGINAL ARTICLE
Increased Serum Interleukin-6 and Tumor Necrosis Factor Alpha Levels in Fabry Disease: Correlation with Disease Burden
Nilton Salles Rosa Neto
, Judith Campos de Barros Bento, Valéria de Falco Caparbo, Rosa Maria Rodrigues Pereira
Corresponding author
Divisao de Reumatologia, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo, SP, BR.
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"Increased Serum Interleukin-6 and Tumor Necrosis Factor Alpha Levels in Fabry Disease: Correlation with Disease Burden" "tieneTextoCompleto" => true "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "Nilton Salles Rosa, Judith Campos de Barros Bento, Valéria de Falco Caparbo, Rosa Maria Rodrigues Pereira" "autores" => array:4 [ 0 => array:5 [ "nombre" => "Nilton Salles" "apellidos" => "Rosa" "sufijo" => "Neto" "email" => array:1 [ 0 => "nsalles@yahoo.com" ] "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "*" "identificador" => "cor1" ] ] ] 1 => array:2 [ "nombre" => "Judith Campos de Barros" "apellidos" => "Bento" ] 2 => array:2 [ "nombre" => "Valéria de Falco" "apellidos" => "Caparbo" ] 3 => array:2 [ "nombre" => "Rosa Maria Rodrigues" "apellidos" => "Pereira" ] ] "afiliaciones" => array:1 [ 0 => array:2 [ "entidad" => "Divisao de Reumatologia, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo, SP, BR." "identificador" => "aff1" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor1" "etiqueta" => "*" "correspondencia" => "Corresponding author." ] ] ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig1" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 522 "Ancho" => 750 "Tamanyo" => 39776 ] ] "descripcion" => array:1 [ "en" => "<p id="spara10" class="elsevierStyleSimplePara elsevierViewall">Serum cytokine levels in FD patients <span class="elsevierStyleItalic">versus</span> those in the control subjects.</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><span id="cesec10" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle60">INTRODUCTION</span><p id="para10" class="elsevierStylePara elsevierViewall">Fabry disease (FD) is an X-linked lysosomal disease caused by pathogenic variants of the <span class="elsevierStyleItalic">GLA</span> gene, which encode a defective alpha-galactosidase A enzyme, contributing to substrate accumulation in several organs, with varying degrees of severity and subsequent loss of organ function (<a class="elsevierStyleCrossRef" href="#bib1">1</a>,<a class="elsevierStyleCrossRef" href="#bib2">2</a>).</p><p id="para20" class="elsevierStylePara elsevierViewall">The natural role of lysosomes includes not only substrate degradation, but also the exocytosis of proteins and vesicles, plasma membrane repair, remodeling and growth, inter-organelle and inter-cellular signaling, metabolic sensing, lipid metabolism, and response to cell injury (<a class="elsevierStyleCrossRef" href="#bib3">3</a>). Moreover, as regulators of the immune system, lysosomes are also active players in antigenic presentation and processing, secretion of perforins, phagocytosis, and release of pro-inflammatory mediators (<a class="elsevierStyleCrossRef" href="#bib4">4</a>). Progressive Gb3 accumulation inside lysosomes is not sufficient to explain all aspects of FD pathology. Nevertheless, it is the starting point for a cascade of cellular events that ultimately disrupts the normal functioning of cells (<a class="elsevierStyleCrossRef" href="#bib1">1</a>,<a class="elsevierStyleCrossRefs" href="#bib4">4-6</a>).</p><p id="para30" class="elsevierStylePara elsevierViewall">After the initial trigger, it is thought that energetic metabolism becomes impaired, oxidative stress attains a pro-oxidative status, and autophagosomes fail to mature efficiently. Eventually, dysfunction of the ionic membrane channels ensues. In due course, injury of small vessels results in tissue ischemia, cell death, and development of irreversible tissue fibrosis, most prominently affecting the heart and kidneys (<a class="elsevierStyleCrossRef" href="#bib1">1</a>,<a class="elsevierStyleCrossRefs" href="#bib7">7-12</a>).</p><p id="para40" class="elsevierStylePara elsevierViewall">In this context, chronic inflammation is thought to contribute to organ damage in patients with FD. Previous reports have evaluated the plasma levels of pro-inflammatory cytokines (<a class="elsevierStyleCrossRef" href="#bib7">7</a>,<a class="elsevierStyleCrossRefs" href="#bib13">13-16</a>), peripheral blood mononuclear cell (PBMC) levels of pro-inflammatory cytokines (<a class="elsevierStyleCrossRef" href="#bib16">16</a>,<a class="elsevierStyleCrossRef" href="#bib17">17</a>), and functional polymorphisms of pro-inflammatory cytokine-encoding genes in patients with FD (<a class="elsevierStyleCrossRef" href="#bib18">18</a>).</p><p id="para50" class="elsevierStylePara elsevierViewall">This study aimed to evaluate the levels of serum inflammatory cytokines in patients with FD, compared to those in healthy controls, and to ascertain their correlations with the Mainz Severity Score Index (MSSI).</p></span><span id="cesec20" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle70">METHODS</span><p id="para60" class="elsevierStylePara elsevierViewall">This is a sub-analysis of the laboratory findings from the protocol “Assessment of Parameters of Bone Metabolism in patients with Fabry Disease,” which was undertaken at the Rheumatology Division, Faculdade de Medicina, Universidade de São Paulo, São Paulo, Brazil. This work was approved by the Faculdade de Medicina da Universidade de São Paulo Ethics Review Board (number 1.464.841) on March 24, 2016. Written informed consent was obtained from all patients. All procedures were in accordance with the ethical standards of the responsible committee on human experimentation and with the Helsinki Declaration of 1975, as revised in 1983.</p><p id="para70" class="elsevierStylePara elsevierViewall">Thirty-six genotype-confirmed classic FD patients - 15 men and 21 women (variants: C142R, A156D, L180F, R227X, W262X, G271A, P293S, and Y264SX) - and 25 age-matched healthy controls (20 men and 8 women) were assessed. The MSSI (<a class="elsevierStyleCrossRef" href="#bib19">19</a>) was established after interviews with the patients and chart review. Because this is a transverse study, it is not possible to have a trend of MSSI values. Data regarding the age at symptom onset, age at diagnosis, and age at treatment initiation have been previously published (<a class="elsevierStyleCrossRef" href="#bib20">20</a>). In summary, the median age at symptom onset was 12.0 years (range, 5-55 years) and 16.5 years (range, 9-66 years) for men and women, respectively. The mean age at diagnosis was 35.5 years for men (range, 8-55 years) and 35.9 years for women (range, 4-71 years). The median time to diagnosis after symptom onset was 20.5 years (range, 1-42 years) for men and 14.0 years (range, 0-52 years) for women (<a class="elsevierStyleCrossRef" href="#bib20">20</a>).</p><p id="para80" class="elsevierStylePara elsevierViewall">Serum interleukin (IL)-6, IL-1β, and tumor necrosis factor (TNF)-α levels were evaluated using the HBNMAG-51K-13 kit. The human Milliplex® assay was performed using the Luminex® technology kit (Millipore Corporation, Billerica, MA, USA), which is a multi-analyte panel used for bone metabolism research and measures the IL-6, IL-1β, and TNF-α levels.</p><span id="cesec30" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle80">Statistical analysis</span><p id="para90" class="elsevierStylePara elsevierViewall">Results are presented as the mean and standard deviation for continuous variables and percentages for categorical variables. Correlations between continuous variables were measured using Pearson's correlation coefficient. Statistical significance was set at <span class="elsevierStyleItalic">p</span><0.05.</p></span></span><span id="cesec40" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle90">RESULTS</span><p id="para100" class="elsevierStylePara elsevierViewall">The mean age (years) of the FD patients was 43.1±15.4 and that of the control subjects was 47.4±12.2 (<span class="elsevierStyleItalic">p</span>>0.05). Twenty-two patients (59.5%) were treated with enzyme replacement therapy (ERT) and no patients were treated with chaperone therapy during the assessment period.</p><p id="para110" class="elsevierStylePara elsevierViewall">When all patients were included in the analysis, the serum levels of IL-6 and TNF-α were significantly higher than those in the healthy controls, as shown in <a class="elsevierStyleCrossRef" href="#fig1">Figure 1</a>. There was no difference in the levels of IL-1β between FD patients and the controls.</p><elsevierMultimedia ident="fig1"></elsevierMultimedia><p id="para120" class="elsevierStylePara elsevierViewall">When patients were separated based on sex, women with FD had significantly higher serum levels of TNF-α than the healthy female controls (<a class="elsevierStyleCrossRef" href="#fig2">Figure 2</a>). In contrast, men with FD had significantly higher serum levels of IL-6 and TNF-α than the healthy male controls (<a class="elsevierStyleCrossRef" href="#fig3">Figure 3</a>).</p><elsevierMultimedia ident="fig2"></elsevierMultimedia><elsevierMultimedia ident="fig3"></elsevierMultimedia><p id="para130" class="elsevierStylePara elsevierViewall">Patients treated with ERT exhibited higher serum IL-6 and TNF-α levels than those not treated with ERT, as shown in <a class="elsevierStyleCrossRef" href="#fig4">Figure 4</a>. No difference was found between the serum IL-1β levels of patients treated with ERT and those that were not. Cytokine levels in patients treated with and without ERT separated based on sex are available in the Appendix.</p><elsevierMultimedia ident="fig4"></elsevierMultimedia><p id="para140" class="elsevierStylePara elsevierViewall">The MSSI separates patients into three categories based on severity: low, moderate, or severe. Six patients showed severe FD (1 women/5 men); 14 patients showed moderate FD (8 women/6 men), and 17 patients showed low-severity FD (12 women/5 men).</p><p id="para150" class="elsevierStylePara elsevierViewall">The mean MSSI score in men with FD who were treated with ERT was not different from that of patients not treated with ERT (29.0±11.1 <span class="elsevierStyleItalic">versus</span> 26.7±14.9, <span class="elsevierStyleItalic">p</span>=0.78). In contrast, in women with FD, the mean score was 25.8±8.2 for those treated with ERT and 10.3±7.1 for those not treated with ERT (<span class="elsevierStyleItalic">p</span>=0.0003).</p><p id="para160" class="elsevierStylePara elsevierViewall">In all patients, the MSSI score correlated with the serum levels of IL-6 (r=0.60, <span class="elsevierStyleItalic">p</span><0.001) and TNF-α levels (r=0.45, <span class="elsevierStyleItalic">p</span><0.001), as shown in <a class="elsevierStyleCrossRef" href="#fig5">Figure 5</a>. However, the serum IL-1β levels did not correlate with the severity score (r=0.02). When separated based on sex, a correlation with the MSSI score was observed only for TNF-α levels (r=0.48, <span class="elsevierStyleItalic">p</span><0.001) in women with FD and only for IL-6 levels (r=0.77, <span class="elsevierStyleItalic">p</span><0.001) in men with FD.</p><elsevierMultimedia ident="fig5"></elsevierMultimedia></span><span id="cesec50" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle100">DISCUSSION</span><p id="para170" class="elsevierStylePara elsevierViewall">The evidence presented in this study reinforces the fact that FD patients have elevated serum levels of pro-inflammatory cytokines. The disease burden, as estimated by the MSSI score, is clearly related to higher levels of IL-6 and TNF-α.</p><p id="para180" class="elsevierStylePara elsevierViewall">The pro-inflammatory state has previously been described in FD. There is an accumulation of Gb3 in leukocytes and subsequent changes in the subpopulations and expression of surface molecules (<a class="elsevierStyleCrossRef" href="#bib4">4</a>,<a class="elsevierStyleCrossRef" href="#bib10">10</a>,<a class="elsevierStyleCrossRef" href="#bib12">12</a>,<a class="elsevierStyleCrossRef" href="#bib21">21</a>). Invariant natural killer T cells (iNKT) recognize the glycosphingolipid Gb3 as an antigen presented by antigen-presenting cells and promote the release of inflammatory cytokines (<a class="elsevierStyleCrossRef" href="#bib22">22</a>,<a class="elsevierStyleCrossRef" href="#bib23">23</a>). In addition, Gb3 may work as an activator of Toll-like receptor 4 (TLR4) and contribute to the deregulation of the activity of both iNKT cells and dendritic cells (<a class="elsevierStyleCrossRef" href="#bib4">4</a>,<a class="elsevierStyleCrossRef" href="#bib16">16</a>,<a class="elsevierStyleCrossRef" href="#bib23">23</a>).</p><p id="para190" class="elsevierStylePara elsevierViewall">Lysosomal deposits of Gb3 may function as damage-associated molecular patterns (DAMPs) or induce the production of DAMPs by damaged cells and induce pro-oxidative and pro-apoptotic patterns (<a class="elsevierStyleCrossRef" href="#bib4">4</a>,<a class="elsevierStyleCrossRef" href="#bib16">16</a>,<a class="elsevierStyleCrossRef" href="#bib23">23</a>).</p><p id="para200" class="elsevierStylePara elsevierViewall">De Francesco et al. (<a class="elsevierStyleCrossRef" href="#bib16">16</a>) reported that unstimulated PBMC from FD patients had higher levels of both IL-1β and TNF-α, whereas IL-6 levels were not different from those of controls. After 24h of cell culture, these authors found the elevated production of IL-1β and IL-6, but not TNF-α. Gb3 was associated with the release of the cytokines IL-1β and TNF-α, but not IL-6, and enhanced the activity of dendritic cells and monocytes. When a TLR4 inhibitor was used, the inflammatory response was blocked, suggesting an interaction between Gb3 and this receptor. The participation of the innate immune system in this response has been highlighted. Our results show elevated serum IL-6 and TNF-α levels in classic FD patients, while the IL-1β levels were unchanged. It is not possible to directly compare cellular expression <span class="elsevierStyleItalic">in vitro</span> to the serum levels <span class="elsevierStyleItalic">in vivo</span> because cytokines are released in a paracrine manner and serum levels may vary depending on several factors, such as receptor binding, temperature-associated degradation, and breakdown within reacting cells when the subject'´s blood sample is drawn (<a class="elsevierStyleCrossRef" href="#bib24">24</a>).</p><p id="para210" class="elsevierStylePara elsevierViewall">Recently, Üçeyler et al. (<a class="elsevierStyleCrossRef" href="#bib17">17</a>) found the increased expression of TNF, IL-1β, and TLR4 in PBMCs from men with FD. However, the secreted TNF levels were only increased in PBMCs from men with FD with pain and classical variants and not in those without pain. Nevertheless, it was demonstrated that heat and lipopolysaccharide stimuli enhance TNF secretion and Gb3 accumulation, linking typical triggers with FD pain.</p><p id="para220" class="elsevierStylePara elsevierViewall">Biancini et al. (<a class="elsevierStyleCrossRef" href="#bib14">14</a>), using a multi-analyte panel similar to that presented herein, evaluated 14 FD patients being treated with ERT and found elevated plasma levels of IL-6 and TNF-α compared to those in controls, and also advocated for the role of Gb3 as a promoter of inflammation. However, the IL-1β levels were not measured in their study. Similarly, we found elevated serum levels of IL-6 and TNF-α, but not IL-1β, in patients with FD. It is noteworthy that both the study by Biancini et al. and our present study included patients being treated with ERT, which may have influenced the observed results. Notably, the elevated levels of IL-6 found in the patients studied herein was largely due to the results from men with FD, but when comparing patients undergoing ERT to those that were not, the elevated IL-6 levels were driven by the women with FD. Sex-associated differences in plasma IL-6 levels are not well understood. The same cut-off value is typically used for both sexes. Pooling of data for men and women with FD may not be accurate, and reference values may require different cut-off values (<a class="elsevierStyleCrossRef" href="#bib25">25</a>). Thus, data from our study were presented as both a pooled analysis and separated based on sex. Overall, our results suggest that patients with more severe disease, and thus, receiving ERT, have higher serum IL-6 and TNF-α levels than untreated patients.</p><p id="para230" class="elsevierStylePara elsevierViewall">The MSSI score is a marker of disease severity. The original work of Whybra et al. (<a class="elsevierStyleCrossRef" href="#bib19">19</a>) showed that FD patients undergoing ERT showed reduced MSSI scores after one year of treatment with agalsidase alfa, particularly on account of general, neurological, and cardiovascular sub-scores. The present study was a transverse assessment of FD patients at different disease time points. The majority of the patients enrolled in this study were cared for by outside providers; they had different access to healthcare and data acquisition at regular time points was not possible for many patients. Although the results for the 24-hour urine protein or microalbumin measurements and echocardiograms were obtained for most patients, we only collected information on broadly defined parameters, as required to calculate the MSSI scores. Thus, it was not possible to assess the trend of how the MSSI scores changed over time, nor to compare the parameters to calculate the Fabry Stabilization Index (FASTEX), a marker of the clinical stabilization of the disease (<a class="elsevierStyleCrossRef" href="#bib26">26</a>).</p><p id="para240" class="elsevierStylePara elsevierViewall">With regard to only women with FD, those undergoing ERT had higher MSSI scores, which reflects the current treatment guidelines, which state that women with FD who are symptomatic or present with organ dysfunction are more likely to be prescribed specific treatment. Therefore, higher serum cytokine levels in this group may be related to a higher disease burden, illustrating the indication for targeted treatment (<a class="elsevierStyleCrossRef" href="#fig6">Figure S1</a>, refer to Appendix). Moreover, the average MSSI scores and serum cytokine levels in men with FD treated with and without ERT were similar. However, only three men with FD were not being treated with ERT, precluding proper analysis (<a class="elsevierStyleCrossRef" href="#fig6">Figure S2</a>, Appendix).</p><elsevierMultimedia ident="fig6"></elsevierMultimedia><p id="para250" class="elsevierStylePara elsevierViewall">Interestingly, Safyan et al. (<a class="elsevierStyleCrossRef" href="#bib17">17</a>) studied polymorphisms of key pro- and anti-inflammatory cytokine-encoding genes in FD, namely, <span class="elsevierStyleItalic">IL-1β</span>, <span class="elsevierStyleItalic">IL-1α</span>, <span class="elsevierStyleItalic">TNF-α</span>, and IL-10. These authors found that most patients had low TNF-α levels and high levels of IL-10, IL-1β, and IL-1α. They described a correlation between the MSSI renal and neurological sub-scores and TNF-α levels (<span class="elsevierStyleItalic">p</span>=0.06) and the MSSI neurological sub-score and IL-10 levels (<span class="elsevierStyleItalic">p</span>=0.03); however, the levels of none of the cytokines were correlated with alpha-galactosidase A levels. Our results indicate the opposite, with patients having elevated serum TNF-α levels and a positive correlation of the levels of this cytokine with higher MSSI scores.</p><p id="para260" class="elsevierStylePara elsevierViewall">Given that patients being treated with ERT in our study presented with elevated levels of pro-inflammatory cytokines, an observation that has not been consistently reported, this raises several questions: a) whether ERT alone fails to adequately suppress the inflammatory process; b) whether ERT itself contributes to the inflammatory process; c) whether specialists should research the possible use of anti-inflammatory or immunomodulatory drugs in the care of patients with FD; and d) whether there is a point of no return after which ERT is no longer beneficial for a specific target organ and the degree of tissue damage (or fibrosis) may contribute to persistent inflammation and lead to organ failure regardless of the treatment. As reviewed by Rozenfeld and Feriozzi (<a class="elsevierStyleCrossRef" href="#bib4">4</a>), fibrosis is a common finding in the heart and kidney of FD patients; it is driven by TGF-β1 and TLR4 activation.</p><p id="para270" class="elsevierStylePara elsevierViewall">This study also has a few limitations. We evaluated the serum levels of these cytokines, while previous publications reported their plasma or PBMC levels; therefore, these values are not directly comparable. As a transverse study, the measurement of cytokine levels was performed only once, and the cytokine levels prior to ERT or at the time of FD diagnosis were not measured. Similarly, the MSSI scores were assessed only once, and it is not possible to evaluate the impact of ERT on these results, to establish a trend of MSSI scores, or to calculate the FASTEX scores. Interpretation of the correlations between the cytokine levels and MSSI scores reported in this study should consider all these aspects. In addition, the low number of untreated men with FD hampered additional comparisons.</p><p id="para280" class="elsevierStylePara elsevierViewall">Nevertheless, a thorough review was performed to ensure the validity of the MSSI scores at the time of assessment. The patients showed different degrees of kidney and heart damage, and several parameters, including hemodialysis, patient status post kidney transplantation, treatment with different immunosuppressive regimens, delayed time to adequate diagnosis, and specific treatment initiation, were considered in this review.</p></span><span id="cesec60" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle110">CONCLUSION</span><p id="para290" class="elsevierStylePara elsevierViewall">In the patient cohort studied herein, FD was associated with elevated serum levels of IL-6 and TNF-α. Patients with FD that received ERT, particularly, women, showed higher levels of serum IL-6 and TNF-α than those that did not receive ERT; the levels of serum IL-6 and TNF-α were correlated with the MSSI scores reflecting a greater disease burden. Further studies assessing the levels of inflammatory cytokines pre- and post-infusion in treatment-naïve patients and comparisons to migalastat-treated patients prior to therapy initiation and after determined timepoints may provide additional information regarding this topic.</p><span id="cesec70" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle120">Conflicts of Interests</span><p id="para300" class="elsevierStylePara elsevierViewall">NSRN declares having received speaker's and advisory board fees from Shire HGT, now Takeda Pharmaceuticals. JCBB declares that their spouse received speaker's and advisory board fees from Shire HGT, now Takeda Pharmaceuticals.</p></span></span><span id="cesec80" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle130">AUTHOR CONTRIBUTIONS</span><p id="para310" class="elsevierStylePara elsevierViewall">Rosa Neto NS designed the study, collected, analyzed, interpreted the data and wrote the manuscript. Bento JCB collected the data. Caparbo VF collected the data. Pereira RMR analyzed and interpreted the data and reviewed the manuscript. All of the authors have read and approved the final version of the manuscript.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:9 [ 0 => array:2 [ "identificador" => "xpalclavsec1577262" "titulo" => "KEYWORDS" ] 1 => array:2 [ "identificador" => "cesec10" "titulo" => "INTRODUCTION" ] 2 => array:3 [ "identificador" => "cesec20" "titulo" => "METHODS" "secciones" => array:1 [ 0 => array:2 [ "identificador" => "cesec30" "titulo" => "Statistical analysis" ] ] ] 3 => array:2 [ "identificador" => "cesec40" "titulo" => "RESULTS" ] 4 => array:2 [ "identificador" => "cesec50" "titulo" => "DISCUSSION" ] 5 => array:3 [ "identificador" => "cesec60" "titulo" => "CONCLUSION" "secciones" => array:1 [ 0 => array:2 [ "identificador" => "cesec70" "titulo" => "Conflicts of Interests" ] ] ] 6 => array:2 [ "identificador" => "cesec80" "titulo" => "AUTHOR CONTRIBUTIONS" ] 7 => array:2 [ "identificador" => "xack636767" "titulo" => "ACKNOWLEDGMENTS" ] 8 => array:1 [ "titulo" => "REFERENCES" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2020-11-25" "PalabrasClave" => array:1 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "KEYWORDS" "identificador" => "xpalclavsec1577262" "palabras" => array:5 [ 0 => "Fabry Disease" 1 => "<span class="elsevierStyleItalic">GLA</span> Gene" 2 => "Inflammation" 3 => "Mainz Severity Score Index" 4 => "Cytokine" ] ] ] ] "tieneResumen" => true "resumen" => array:1 [ "en" => array:2 [ "resumen" => "<span id="ceabs10" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle10">OBJECTIVES:</span><p id="spara60" class="elsevierStyleSimplePara elsevierViewall">Fabry disease (FD) is an X-linked lysosomal disease caused by variants of the <span class="elsevierStyleItalic">GLA</span> gene; the formation of defective alpha-galactosidase A contributes to the accumulation of substrates in several organs. Chronic inflammation is thought to contribute to organ damage in FD patients.</p></span> <span id="ceabs20" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle20">METHODS:</span><p id="spara70" class="elsevierStyleSimplePara elsevierViewall">In total, 36 classic FD patients (15 men/21 women) and 25 healthy controls (20 men/8 women) were assessed. The Mainz Severity Score Index (MSSI) was established after conducting interviews with the patients and chart review. Serum IL-6, IL-1β, and TNF-α levels were evaluated in both groups.</p></span> <span id="ceabs30" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle30">RESULTS:</span><p id="spara80" class="elsevierStyleSimplePara elsevierViewall">The mean age (years) for FD patients was 43.1±15.4 and that for the controls was 47.4±12.2 (<span class="elsevierStyleItalic">p</span>>0.05). Twenty-two patients (59.5%) were treated with enzyme replacement therapy (ERT). Serum IL-6 and TNF-α levels were significantly higher in FD patients than in the controls. Patients treated with ERT had higher serum IL-6 and TNF-α levels than those not treated with ERT. There was no difference in the serum IL-1β levels between patients treated with ERT and those who were not. The MSSI scores in the patients were correlated with serum levels of IL-6 (r=0.60, <span class="elsevierStyleItalic">p</span><0.001) and TNF-α (r=0.45, <span class="elsevierStyleItalic">p</span><0.001).</p></span> <span id="ceabs40" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle40">CONCLUSION:</span><p id="spara90" class="elsevierStyleSimplePara elsevierViewall">FD was associated with elevated serum levels of IL-6 and TNF-α in this cohort. The FD patients treated with ERT, particularly, women, exhibited higher levels of serum IL-6 and TNF-α than those not treated with ERT; the serum IL-6 and TNF-α levels were correlated with the MSSI scores reflecting greater disease burden.</p></span>" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "ceabs10" "titulo" => "OBJECTIVES:" ] 1 => array:2 [ "identificador" => "ceabs20" "titulo" => "METHODS:" ] 2 => array:2 [ "identificador" => "ceabs30" "titulo" => "RESULTS:" ] 3 => array:2 [ "identificador" => "ceabs40" "titulo" => "CONCLUSION:" ] ] ] ] "apendice" => array:1 [ 0 => array:1 [ "seccion" => array:1 [ 0 => array:3 [ "apendice" => "<p id="para330" class="elsevierStylePara elsevierViewall"><elsevierMultimedia ident="fig6"></elsevierMultimedia></p> <p id="para340" class="elsevierStylePara elsevierViewall"><elsevierMultimedia ident="fig7"></elsevierMultimedia></p>" "titulo" => "APPENDIX" "identificador" => "cesec90" ] ] ] ] "multimedia" => array:7 [ 0 => array:7 [ "identificador" => "fig1" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 522 "Ancho" => 750 "Tamanyo" => 39776 ] ] "descripcion" => array:1 [ "en" => "<p id="spara10" class="elsevierStyleSimplePara elsevierViewall">Serum cytokine levels in FD patients <span class="elsevierStyleItalic">versus</span> those in the control subjects.</p>" ] ] 1 => array:7 [ "identificador" => "fig2" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 550 "Ancho" => 750 "Tamanyo" => 39320 ] ] "descripcion" => array:1 [ "en" => "<p id="spara20" class="elsevierStyleSimplePara elsevierViewall">Serum cytokine levels in women with FD <span class="elsevierStyleItalic">versus</span> those in the control subjects.</p>" ] ] 2 => array:7 [ "identificador" => "fig3" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 556 "Ancho" => 750 "Tamanyo" => 37858 ] ] "descripcion" => array:1 [ "en" => "<p id="spara30" class="elsevierStyleSimplePara elsevierViewall">Serum cytokine levels in men with FD <span class="elsevierStyleItalic">versus</span> those in the control subjects.</p>" ] ] 3 => array:7 [ "identificador" => "fig4" "etiqueta" => "Figure 4" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr4.jpeg" "Alto" => 555 "Ancho" => 750 "Tamanyo" => 39943 ] ] "descripcion" => array:1 [ "en" => "<p id="spara40" class="elsevierStyleSimplePara elsevierViewall">Serum cytokine levels in FD patients treated with ERT <span class="elsevierStyleItalic">versus</span> those not treated with ERT.</p>" ] ] 4 => array:7 [ "identificador" => "fig5" "etiqueta" => "Figure 5" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr5.jpeg" "Alto" => 506 "Ancho" => 700 "Tamanyo" => 60419 ] ] "descripcion" => array:1 [ "en" => "<p id="spara50" class="elsevierStyleSimplePara elsevierViewall">Correlations between MSSI scores and serum cytokine levels in men and women with FD.</p>" ] ] 5 => array:7 [ "identificador" => "fig6" "etiqueta" => "Figure S1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => false "mostrarDisplay" => true "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr6.jpeg" "Alto" => 520 "Ancho" => 750 "Tamanyo" => 38466 ] ] "descripcion" => array:1 [ "en" => "<p id="spara100" class="elsevierStyleSimplePara elsevierViewall">Serum cytokine levels in women with FD treated with ERT <span class="elsevierStyleItalic">versus</span> those not treated with ERT.</p>" ] ] 6 => array:7 [ "identificador" => "fig7" "etiqueta" => "Figure S2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => false "mostrarDisplay" => true "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr7.jpeg" "Alto" => 504 "Ancho" => 750 "Tamanyo" => 37815 ] ] "descripcion" => array:1 [ "en" => "<p id="spara110" class="elsevierStyleSimplePara elsevierViewall">Serum cytokine levels in men with FD treated with ERT <span class="elsevierStyleItalic">versus</span> those not treated with ERT.</p>" ] ] ] "bibliografia" => array:2 [ "titulo" => "REFERENCES" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "cebibsec10" "bibliografiaReferencia" => array:26 [ 0 => array:3 [ "identificador" => "bib1" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Fabry disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => """ DP Germain \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1186/1750-1172-5-30" "Revista" => array:5 [ "tituloSerie" => "Orphanet J Rare Dis" "fecha" => "2010" "volumen" => "5" "paginaInicial" => "30" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/21092187" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1186/1750-1172-5-30" "WWW" => array:1 [ "link" => "https://doi.org/10.1186/1750-1172-5-30" ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib2" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Fabry disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => """ R Schiffmann \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1016/B978-0-444-62702-5.00017-2" "Revista" => array:5 [ "tituloSerie" => "Handb Clin Neurol" "fecha" => "2015" "volumen" => "132" "paginaInicial" => "231" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26564084" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1016/B978-0-444-62702-5.00017-2" "WWW" => array:1 [ "link" => "https://doi.org/10.1016/B978-0-444-62702-5.00017-2" ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bib3" "etiqueta" => "3" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Lysosome function in glomerular health and disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => """ C Meyer-Schwesinger \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1007/s00441-012-1441-5" "Revista" => array:3 [ "tituloSerie" => "Cell Tissue Res" "fecha" => "2021" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22729488" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1007/s00441-012-1441-5" "WWW" => array:1 [ "link" => "https://doi.org/10.1007/s00441-020-03375-7" ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bib4" "etiqueta" => "4" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Contribution of inflammatory pathways to Fabry disease pathogenesis" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:2 [ 0 => """ P Rozenfeld \n \t\t\t\t\t\t\t\t """ 1 => """ S Feriozzi \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1016/j.ymgme.2017.09.004" "Revista" => array:7 [ "tituloSerie" => "Mol Genet Metab" "fecha" => "2017" "volumen" => "122" "numero" => "3" "paginaInicial" => "19" "paginaFinal" => "27" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/28947349" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1016/j.ymgme.2017.09.004" "WWW" => array:1 [ "link" => "https://doi.org/10.1016/j.ymgme.2017.09.004" ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bib5" "etiqueta" => "5" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Immune system irregularities in lysosomal storage disorders" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:4 [ 0 => """ JA Castaneda \n \t\t\t\t\t\t\t\t """ 1 => """ MJ Lim \n \t\t\t\t\t\t\t\t """ 2 => """ JD Cooper \n \t\t\t\t\t\t\t\t """ 3 => """ DA Pearce \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1007/s00401-007-0296-4" "Revista" => array:7 [ "tituloSerie" => "Acta Neuropathol" "fecha" => "2008" "volumen" => "115" "numero" => "2" "paginaInicial" => "159" "paginaFinal" => "174" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17924126" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1007/s00401-007-0296-4" "WWW" => array:1 [ "link" => "https://doi.org/10.1007/s00401-007-0296-4" ] ] ] ] ] ] 5 => array:3 [ "identificador" => "bib6" "etiqueta" => "6" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Pathogenic cascades in lysosomal disease-Why so complex?" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:1 [ 0 => """ SU Walkley \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1007/s10545-008-1040-5" "Revista" => array:7 [ "tituloSerie" => "J Inherit Metab Dis" "fecha" => "2009" "volumen" => "32" "numero" => "2" "paginaInicial" => "181" "paginaFinal" => "189" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19130290" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1007/s10545-008-1040-5" "WWW" => array:1 [ "link" => "https://doi.org/10.1007/s10545-008-1040-5" ] ] ] ] ] ] 6 => array:3 [ "identificador" => "bib7" "etiqueta" => "7" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "DNA damage in Fabry patients: An investigation of oxidative damage and repair" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ GB Biancini \n \t\t\t\t\t\t\t\t """ 1 => """ DJ Moura \n \t\t\t\t\t\t\t\t """ 2 => """ PR Manini \n \t\t\t\t\t\t\t\t """ 3 => """ JL Faverzani \n \t\t\t\t\t\t\t\t """ 4 => """ CB Netto \n \t\t\t\t\t\t\t\t """ 5 => """ M Deon \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1016/j.mrgentox.2015.04.012" "Revista" => array:6 [ "tituloSerie" => "Mutat Res Genet Toxicol Environ Mutagen" "fecha" => "2015" "volumen" => "784-785" "paginaInicial" => "31" "paginaFinal" => "36" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26046974" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1016/j.mrgentox.2015.04.012" "WWW" => array:1 [ "link" => "https://doi.org/10.1016/j.mrgentox.2015.04.012" ] ] ] ] ] ] 7 => array:3 [ "identificador" => "bib8" "etiqueta" => "8" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Overview of immune abnormalities in lysosomal storage disorders" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => """ D Rigante \n \t\t\t\t\t\t\t\t """ 1 => """ C Cipolla \n \t\t\t\t\t\t\t\t """ 2 => """ U Basile \n \t\t\t\t\t\t\t\t """ 3 => """ F Gulli \n \t\t\t\t\t\t\t\t """ 4 => """ MC Savastano \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1016/j.imlet.2017.07.004" "Revista" => array:6 [ "tituloSerie" => "Immunol Lett" "fecha" => "2017" "volumen" => "188" "paginaInicial" => "79" "paginaFinal" => "85" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/28687233" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1016/j.imlet.2017.07.004" "WWW" => array:1 [ "link" => "https://doi.org/10.1016/j.imlet.2017.07.004" ] ] ] ] ] ] 8 => array:3 [ "identificador" => "bib9" "etiqueta" => "9" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Biomolecules damage and redox status abnormalities in Fabry patients before and during enzyme replacement therapy" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ GB Biancini \n \t\t\t\t\t\t\t\t """ 1 => """ CE Jacques \n \t\t\t\t\t\t\t\t """ 2 => """ T Hammerschmidt \n \t\t\t\t\t\t\t\t """ 3 => """ HM de Souza \n \t\t\t\t\t\t\t\t """ 4 => """ B Donida \n \t\t\t\t\t\t\t\t """ 5 => """ M Deon \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1016/j.cca.2016.07.016" "Revista" => array:6 [ "tituloSerie" => "Clin Chim Acta" "fecha" => "2016" "volumen" => "461" "paginaInicial" => "41" "paginaFinal" => "46" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/27458128" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1016/j.cca.2016.07.016" "WWW" => array:1 [ "link" => "https://doi.org/10.1016/j.cca.2016.07.016" ] ] ] ] ] ] 9 => array:3 [ "identificador" => "bib10" "etiqueta" => "10" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Globotriaosylceramide induces oxidative stress and up-regulates cell adhesion molecule expression in Fabry disease endothelial cells" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ JS Shen \n \t\t\t\t\t\t\t\t """ 1 => """ XL Meng \n \t\t\t\t\t\t\t\t """ 2 => """ DF Moore \n \t\t\t\t\t\t\t\t """ 3 => """ JM Quirk \n \t\t\t\t\t\t\t\t """ 4 => """ JA Shayman \n \t\t\t\t\t\t\t\t """ 5 => """ R Schiffmann \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1016/j.ymgme.2008.06.016" "Revista" => array:7 [ "tituloSerie" => "Mol Genet Metab" "fecha" => "2008" "volumen" => "95" "numero" => "3" "paginaInicial" => "163" "paginaFinal" => "168" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18707907" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1016/j.ymgme.2008.06.016" "WWW" => array:1 [ "link" => "https://doi.org/10.1016/j.ymgme.2008.06.016" ] ] ] ] ] ] 10 => array:3 [ "identificador" => "bib11" "etiqueta" => "11" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Profile of endothelial and leukocyte activation in Fabry patients" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ T DeGraba \n \t\t\t\t\t\t\t\t """ 1 => """ S Azhar \n \t\t\t\t\t\t\t\t """ 2 => """ F Dignat-George \n \t\t\t\t\t\t\t\t """ 3 => """ E Brown \n \t\t\t\t\t\t\t\t """ 4 => """ B Boutiàre \n \t\t\t\t\t\t\t\t """ 5 => """ G Altarescu \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:1 [ "Revista" => array:7 [ "tituloSerie" => "Ann Neurol" "fecha" => "2000" "volumen" => "47" "numero" => "2" "paginaInicial" => "229" "paginaFinal" => "233" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10665494" "web" => "Medline" ] ] ] ] 1 => array:1 [ "WWW" => array:1 [ "link" => "https://doi.org/10.1002/1531-8249(200002)47:2<229::AID-ANA13>3.0.CO;2-T" ] ] ] ] ] ] 11 => array:3 [ "identificador" => "bib12" "etiqueta" => "12" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Higher apoptotic state in Fabry disease peripheral blood mononuclear cells: effect of globotriaosylceramide" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => """ PN De Francesco \n \t\t\t\t\t\t\t\t """ 1 => """ JM Mucci \n \t\t\t\t\t\t\t\t """ 2 => """ R Ceci \n \t\t\t\t\t\t\t\t """ 3 => """ CA Fossati \n \t\t\t\t\t\t\t\t """ 4 => """ PA Rozenfeld \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1016/j.ymgme.2011.06.007" "Revista" => array:8 [ "tituloSerie" => "Mol Genet Metab" "fecha" => "2011" "volumen" => "104" "numero" => "3" "paginaInicial" => "319" "paginaFinal" => "324" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/21724436" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S0923753419377907" "estado" => "S300" "issn" => "09237534" ] ] ] 1 => array:2 [ "doi" => "10.1016/j.ymgme.2011.06.007" "WWW" => array:1 [ "link" => "https://doi.org/10.1016/j.ymgme.2011.06.007" ] ] ] ] ] ] 12 => array:3 [ "identificador" => "bib13" "etiqueta" => "13" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Elevated Inflammatory Plasma Biomarkers in Patients With Fabry Disease: A Critical Link to Heart Failure With Preserved Ejection Fraction" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ H Yogasundaram \n \t\t\t\t\t\t\t\t """ 1 => """ A Nikhanj \n \t\t\t\t\t\t\t\t """ 2 => """ BN Putko \n \t\t\t\t\t\t\t\t """ 3 => """ M Boutin \n \t\t\t\t\t\t\t\t """ 4 => """ S Jain-Ghai \n \t\t\t\t\t\t\t\t """ 5 => """ A Khan \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1161/JAHA.118.008528" "Revista" => array:5 [ "tituloSerie" => "J Am Heart Assoc" "fecha" => "2018" "volumen" => "7" "numero" => "21" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/29674334" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1161/JAHA.118.008528" "WWW" => array:1 [ "link" => "https://doi.org/10.1161/JAHA.118.009098" ] ] ] ] ] ] 13 => array:3 [ "identificador" => "bib14" "etiqueta" => "14" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Globotriaosylceramide is correlated with oxidative stress and inflammation in Fabry patients treated with enzyme replacement therapy" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ GB Biancini \n \t\t\t\t\t\t\t\t """ 1 => """ CS Vanzin \n \t\t\t\t\t\t\t\t """ 2 => """ DB Rodrigues \n \t\t\t\t\t\t\t\t """ 3 => """ M Deon \n \t\t\t\t\t\t\t\t """ 4 => """ GS Ribas \n \t\t\t\t\t\t\t\t """ 5 => """ AG Barschak \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1016/j.bbadis.2011.11.001" "Revista" => array:7 [ "tituloSerie" => "Biochim Biophys Acta" "fecha" => "2012" "volumen" => "1822" "numero" => "2" "paginaInicial" => "226" "paginaFinal" => "232" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22085605" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1016/j.bbadis.2011.11.001" "WWW" => array:1 [ "link" => "https://doi.org/10.1016/j.bbadis.2011.11.001" ] ] ] ] ] ] 14 => array:3 [ "identificador" => "bib15" "etiqueta" => "15" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Evaluation of Proinflammatory Prognostic Biomarkers for Fabry Cardiomyopathy With Enzyme Replacement Therapy" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ KH Chen \n \t\t\t\t\t\t\t\t """ 1 => """ Y Chien \n \t\t\t\t\t\t\t\t """ 2 => """ KL Wang \n \t\t\t\t\t\t\t\t """ 3 => """ HB Leu \n \t\t\t\t\t\t\t\t """ 4 => """ CY Hsiao \n \t\t\t\t\t\t\t\t """ 5 => """ YH Lai \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1016/j.cjca.2016.01.010" "Revista" => array:7 [ "tituloSerie" => "Can J Cardiol" "fecha" => "2016" "volumen" => "32" "numero" => "10" "paginaInicial" => "1221.e1" "paginaFinal" => "1221.e9" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/26975225" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1016/j.cjca.2016.01.010" "WWW" => array:1 [ "link" => "https://doi.org/10.1016/j.cjca.2015.10.033" ] ] ] ] ] ] 15 => array:3 [ "identificador" => "bib16" "etiqueta" => "16" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Fabry disease peripheral blood immune cells release inflammatory cytokines: role of globotriaosylceramide" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => """ PN De Francesco \n \t\t\t\t\t\t\t\t """ 1 => """ JM Mucci \n \t\t\t\t\t\t\t\t """ 2 => """ R Ceci \n \t\t\t\t\t\t\t\t """ 3 => """ CA Fossati \n \t\t\t\t\t\t\t\t """ 4 => """ PA Rozenfeld \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1016/j.ymgme.2013.02.003" "Revista" => array:8 [ "tituloSerie" => "Mol Genet Metab" "fecha" => "2013" "volumen" => "109" "numero" => "1" "paginaInicial" => "93" "paginaFinal" => "99" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23452955" "web" => "Medline" ] ] "itemHostRev" => array:3 [ "pii" => "S0165587619303866" "estado" => "S300" "issn" => "01655876" ] ] ] 1 => array:2 [ "doi" => "10.1016/j.ymgme.2013.02.003" "WWW" => array:1 [ "link" => "https://doi.org/10.1016/j.ymgme.2013.02.003" ] ] ] ] ] ] 16 => array:3 [ "identificador" => "bib17" "etiqueta" => "17" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Tumor necrosis factor-α links heat and inflammation with Fabry pain" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => """ N Üçeyler \n \t\t\t\t\t\t\t\t """ 1 => """ D Urlaub \n \t\t\t\t\t\t\t\t """ 2 => """ C Mayer \n \t\t\t\t\t\t\t\t """ 3 => """ S Uehlein \n \t\t\t\t\t\t\t\t """ 4 => """ M Held \n \t\t\t\t\t\t\t\t """ 5 => """ C Sommer \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1016/j.ymgme.2019.05.009" "Revista" => array:7 [ "tituloSerie" => "Mol Genet Metab" "fecha" => "2019" "volumen" => "127" "numero" => "3" "paginaInicial" => "200" "paginaFinal" => "206" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/31221509" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1016/j.ymgme.2019.05.009" "WWW" => array:1 [ "link" => "https://doi.org/10.1016/j.ymgme.2019.05.009" ] ] ] ] ] ] 17 => array:3 [ "identificador" => "bib18" "etiqueta" => "18" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "An association study of inflammatory cytokine gene polymorphisms in Fabry disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => """ R Safyan \n \t\t\t\t\t\t\t\t """ 1 => """ C Whybra \n \t\t\t\t\t\t\t\t """ 2 => """ M Beck \n \t\t\t\t\t\t\t\t """ 3 => """ D Elstein \n \t\t\t\t\t\t\t\t """ 4 => """ G Altarescu \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:1 [ "Revista" => array:7 [ "tituloSerie" => "Eur Cytokine Netw" "fecha" => "2006" "volumen" => "17" "numero" => "4" "paginaInicial" => "271" "paginaFinal" => "275" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17353161" "web" => "Medline" ] ] ] ] 1 => array:1 [ "WWW" => array:1 [ "link" => "https://doi.org/10.1684/ecn.2006.0043" ] ] ] ] ] ] 18 => array:3 [ "identificador" => "bib19" "etiqueta" => "19" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The Mainz Severity Score Index: a new instrument for quantifying the Anderson-Fabry disease phenotype, and the response of patients to enzyme replacement therapy" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ C Whybra \n \t\t\t\t\t\t\t\t """ 1 => """ C Kampmann \n \t\t\t\t\t\t\t\t """ 2 => """ F Krummenauer \n \t\t\t\t\t\t\t\t """ 3 => """ M Ries \n \t\t\t\t\t\t\t\t """ 4 => """ E Mengel \n \t\t\t\t\t\t\t\t """ 5 => """ E Miebach \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1111/j.1399-0004.2004.00219.x" "Revista" => array:7 [ "tituloSerie" => "Clin Genet" "fecha" => "2004" "volumen" => "65" "numero" => "4" "paginaInicial" => "299" "paginaFinal" => "307" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15025723" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1111/j.1399-0004.2004.00219.x" "WWW" => array:1 [ "link" => "https://doi.org/10.1111/j.1399-0004.2004.00219.x" ] ] ] ] ] ] 19 => array:3 [ "identificador" => "bib20" "etiqueta" => "20" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Higher rate of rheumatic manifestations and delay in diagnosis in Brazilian Fabry disease patients" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => """ NS Rosa Neto \n \t\t\t\t\t\t\t\t """ 1 => """ JCB Bento \n \t\t\t\t\t\t\t\t """ 2 => """ RMR Pereira \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1186/s42358-019-0111-7" "Revista" => array:6 [ "tituloSerie" => "Adv Rheumatol" "fecha" => "2020" "volumen" => "60" "numero" => "1" "paginaInicial" => "7" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/31907047" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1186/s42358-019-0111-7" "WWW" => array:1 [ "link" => "https://doi.org/10.1186/s42358-019-0111-7" ] ] ] ] ] ] 20 => array:3 [ "identificador" => "bib21" "etiqueta" => "21" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Leukocyte perturbation associated with Fabry disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => """ P Rozenfeld \n \t\t\t\t\t\t\t\t """ 1 => """ E Agriello \n \t\t\t\t\t\t\t\t """ 2 => """ N De Francesco \n \t\t\t\t\t\t\t\t """ 3 => """ P Martinez \n \t\t\t\t\t\t\t\t """ 4 => """ C Fossati \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1007/s10545-009-1060-9" "Revista" => array:7 [ "tituloSerie" => "J Inherit Metab Dis" "fecha" => "2009" "volumen" => "32" "numero" => "Suppl 1" "paginaInicial" => "S67" "paginaFinal" => "S77" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19259782" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1007/s10545-009-1060-9" "WWW" => array:1 [ "link" => "https://doi.org/10.1007/s10545-009-1060-9" ] ] ] ] ] ] 21 => array:3 [ "identificador" => "bib22" "etiqueta" => "22" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Invariant natural killer T cells are phenotypically and functionally altered in Fabry disease" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => """ CS Pereira \n \t\t\t\t\t\t\t\t """ 1 => """ O Azevedo \n \t\t\t\t\t\t\t\t """ 2 => """ ML Maia \n \t\t\t\t\t\t\t\t """ 3 => """ AF Dias \n \t\t\t\t\t\t\t\t """ 4 => """ C Sa-Miranda \n \t\t\t\t\t\t\t\t """ 5 => """ MF Macedo \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1016/j.ymgme.2013.01.018" "Revista" => array:7 [ "tituloSerie" => "Mol Genet Metab" "fecha" => "2013" "volumen" => "108" "numero" => "4" "paginaInicial" => "241" "paginaFinal" => "248" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/23433711" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1016/j.ymgme.2013.01.018" "WWW" => array:1 [ "link" => "https://doi.org/10.1016/j.ymgme.2013.01.018" ] ] ] ] ] ] 22 => array:3 [ "identificador" => "bib23" "etiqueta" => "23" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Innate and Adaptive Immune Response in Fabry Disease" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ W Mauhin \n \t\t\t\t\t\t\t\t """ 1 => """ O Lidove \n \t\t\t\t\t\t\t\t """ 2 => """ E Masat \n \t\t\t\t\t\t\t\t """ 3 => """ F Mingozzi \n \t\t\t\t\t\t\t\t """ 4 => """ K Mariampillai \n \t\t\t\t\t\t\t\t """ 5 => """ JM Ziza \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1007/8904_2014_371" "Revista" => array:6 [ "tituloSerie" => "JIMD Rep" "fecha" => "2015" "volumen" => "22" "paginaInicial" => "1" "paginaFinal" => "10" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/25690728" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1007/8904_2014_371" "WWW" => array:1 [ "link" => "https://doi.org/10.1007/978-3-662-47453-2" ] ] ] ] ] ] 23 => array:3 [ "identificador" => "bib24" "etiqueta" => "24" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Comparison of serum and cell-specific cytokines in humans" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ J Jason \n \t\t\t\t\t\t\t\t """ 1 => """ LK Archibald \n \t\t\t\t\t\t\t\t """ 2 => """ OC Nwanyanwu \n \t\t\t\t\t\t\t\t """ 3 => """ MG Byrd \n \t\t\t\t\t\t\t\t """ 4 => """ PN Kazembe \n \t\t\t\t\t\t\t\t """ 5 => """ H Dobbie \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1128/CDLI.8.6.1097-1103.2001" "Revista" => array:7 [ "tituloSerie" => "Clin Diagn Lab Immunol" "fecha" => "2001" "volumen" => "8" "numero" => "6" "paginaInicial" => "1097" "paginaFinal" => "1103" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/11687446" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1128/CDLI.8.6.1097-1103.2001" "WWW" => array:1 [ "link" => "https://doi.org/10.1128/CDLI.8.6.1097-1103.2001" ] ] ] ] ] ] 24 => array:3 [ "identificador" => "bib25" "etiqueta" => "25" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Gender difference in the effects of interleukin-6 on grip strength - a systematic review and meta-analysis" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ A Mikó \n \t\t\t\t\t\t\t\t """ 1 => """ L Pótó \n \t\t\t\t\t\t\t\t """ 2 => """ P Mátrai \n \t\t\t\t\t\t\t\t """ 3 => """ P Hegyi \n \t\t\t\t\t\t\t\t """ 4 => """ N Füredi \n \t\t\t\t\t\t\t\t """ 5 => """ A Garami \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1186/s12877-018-0798-z" "Revista" => array:6 [ "tituloSerie" => "BMC Geriatr" "fecha" => "2018" "volumen" => "18" "numero" => "1" "paginaInicial" => "107" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/29739343" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1186/s12877-018-0798-z" "WWW" => array:1 [ "link" => "https://doi.org/10.1186/s12877-018-0798-z" ] ] ] ] ] ] 25 => array:3 [ "identificador" => "bib26" "etiqueta" => "26" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "FAbry STabilization indEX (FASTEX): an innovative tool for the assessment of clinical stabilization in Fabry disease" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ R Mignani \n \t\t\t\t\t\t\t\t """ 1 => """ F Pieruzzi \n \t\t\t\t\t\t\t\t """ 2 => """ F Berri \n \t\t\t\t\t\t\t\t """ 3 => """ A Burlina \n \t\t\t\t\t\t\t\t """ 4 => """ B Chinea \n \t\t\t\t\t\t\t\t """ 5 => """ M Gallieni \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1093/ckj/sfw082" "Revista" => array:7 [ "tituloSerie" => "Clin Kidney J" "fecha" => "2016" "volumen" => "9" "numero" => "5" "paginaInicial" => "739" "paginaFinal" => "747" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/27679722" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1093/ckj/sfw082" "WWW" => array:1 [ "link" => "https://doi.org/10.1093/ckj/sfw082" ] ] ] ] ] ] ] ] ] ] "agradecimientos" => array:1 [ 0 => array:4 [ "identificador" => "xack636767" "titulo" => "ACKNOWLEDGMENTS" "texto" => "<p id="para320" class="elsevierStylePara elsevierViewall">The authors would like to acknowledge Rachel Sayuri Honjo Kawahira, Jaelson Guilhem Gomes, Ana Carolina de Paula, and Rosiane Lacerda for the referral of the FD patients. This study was funded by the Brazilian Society of Rheumatology Research Fund. The Brazilian Society of Rheumatology had no role in the design of the study, collection, analysis, and interpretation of data, or in writing the manuscript.</p>" "vista" => "all" ] ] ] "idiomaDefecto" => "en" "url" => "/18075932/000000760000000C/v1_202211191031/S180759322200134X/v1_202211191031/en/main.assets" "Apartado" => null "PDF" => "https://static.elsevier.es/multimedia/18075932/000000760000000C/v1_202211191031/S180759322200134X/v1_202211191031/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S180759322200134X?idApp=UINPBA00004N" ]
| Year/Month | Html | Total | |
|---|---|---|---|
| 2024 November | 2 | 2 | 4 |
| 2024 October | 54 | 23 | 77 |
| 2024 September | 41 | 20 | 61 |
| 2024 August | 40 | 18 | 58 |
| 2024 July | 38 | 18 | 56 |
| 2024 June | 29 | 25 | 54 |
| 2024 May | 28 | 11 | 39 |
| 2024 April | 49 | 22 | 71 |
| 2024 March | 42 | 16 | 58 |
| 2024 February | 53 | 16 | 69 |
| 2024 January | 23 | 12 | 35 |
| 2023 December | 17 | 19 | 36 |
| 2023 November | 26 | 22 | 48 |
| 2023 October | 32 | 31 | 63 |
| 2023 September | 28 | 22 | 50 |
| 2023 August | 41 | 9 | 50 |
| 2023 July | 20 | 29 | 49 |
| 2023 June | 38 | 16 | 54 |
| 2023 May | 54 | 1 | 55 |
| 2023 April | 40 | 1 | 41 |
| 2023 March | 33 | 3 | 36 |
| 2023 February | 25 | 0 | 25 |
| 2023 January | 21 | 2 | 23 |
| 2022 December | 7 | 4 | 11 |
| 2022 November | 7 | 2 | 9 |
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