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This figure describes the cumulative mortality stratified by the D-dimer cut-off (greater or lower than 4,000 ng/mL FEU), with higher cumulative mortality observed in patients with high D-dimer levels. DD=N[0, 4,000]: D-dimer levels up to 4,000 ng/mL FEU; DD=A(4,000, +): D-dimer levels greater than 4,000 ng/mL FEU.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Fabio Augusto Rodrigues Gonçalves, Bruno Adler Maccagnan Pinheiro Besen, Clarice Antunes de Lima, Aline Pivetta Corá, Antônio José Rodrigues Pereira, Sandro Félix Perazzio, Christiane Pereira Gouvea, Luiz Augusto Marcondes Fonseca, Evelinda Marramon Trindade, Nairo Massakazu Sumita, Alberto José da Silva Duarte, Arnaldo Lichtenstein" "autores" => array:13 [ 0 => array:2 [ "nombre" => "Fabio Augusto Rodrigues" "apellidos" => "Gonçalves" ] 1 => array:2 [ "nombre" => "Bruno Adler Maccagnan Pinheiro" "apellidos" => "Besen" ] 2 => array:2 [ "nombre" => "Clarice Antunes de" "apellidos" => "Lima" ] 3 => array:2 [ "nombre" => "Aline Pivetta" "apellidos" => "Corá" ] 4 => array:2 [ "nombre" => "Antônio José Rodrigues" "apellidos" => "Pereira" ] 5 => array:2 [ "nombre" => "Sandro Félix" "apellidos" => "Perazzio" ] 6 => array:2 [ "nombre" => "Christiane Pereira" "apellidos" => "Gouvea" ] 7 => array:2 [ "nombre" => "Luiz Augusto Marcondes" "apellidos" => "Fonseca" ] 8 => array:2 [ "nombre" => "Evelinda Marramon" "apellidos" => "Trindade" ] 9 => array:2 [ "nombre" => "Nairo Massakazu" "apellidos" => "Sumita" ] 10 => array:2 [ "nombre" => "Alberto José da Silva" "apellidos" => "Duarte" ] 11 => array:2 [ "nombre" => "Arnaldo" "apellidos" => "Lichtenstein" ] 12 => array:1 [ "colaborador" => "HCFMUSP COVID-19 Study Group" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1807593222002630?idApp=UINPBA00004N" "url" => "/18075932/000000760000000C/v1_202211191031/S1807593222002630/v1_202211191031/en/main.assets" ] "itemAnterior" => array:19 [ "pii" => "S1807593222002617" "issn" => "18075932" "doi" => "10.6061/clinics/2021/e3540" "estado" => "S300" "fechaPublicacion" => "2021-01-01" "aid" => "261" "copyright" => "CLINICS" "documento" => "article" "crossmark" => 0 "licencia" => "https://creativecommons.org/licenses/by/4.0/" "subdocumento" => "fla" "cita" => "Clinics. 2021;76C:" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:1 [ "total" => 0 ] "en" => array:11 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">ORIGINAL ARTICLE</span>" "titulo" => "Physical and pulmonary capacities of individuals with severe coronavirus disease after hospital discharge: A preliminary cross-sectional study based on cluster analysis" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => "en" "contieneResumen" => array:1 [ "en" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig2" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 840 "Ancho" => 1712 "Tamanyo" => 141840 ] ] "descripcion" => array:1 [ "en" => "<p id="spara20" class="elsevierStyleSimplePara elsevierViewall">Box plot of tests by Cluster with indication of means (in red circles) and <span class="elsevierStyleItalic">p</span>-values for the Student's t-test. SPPB, short physical performance battery; BMI, body mass index; D, dominant; ND, no dominant; TUG, Timed Up and Go; TUG-COG, Timed Up and Go Cognitive; FEV1, forced expiratory volume in 1 second; FVC, forced vital capacity.</p>" ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Angelica Castilho Alonso, Paulo Roberto Silva-Santos, Marília Simões Lopes Quintana, Vanderlei Carneiro da Silva, Guilherme Carlos Brech, Lorena Gonçalves Barbosa, José Eduardo Pompeu, Erika Christina Gouveia e Silva, Elizabeth Mendes da Silva, Caroline Gil de Godoy, Julia Maria D'Andréa Greve" "autores" => array:11 [ 0 => array:2 [ "nombre" => "Angelica Castilho" "apellidos" => "Alonso" ] 1 => array:2 [ "nombre" => "Paulo Roberto" "apellidos" => "Silva-Santos" ] 2 => array:2 [ "nombre" => "Marília Simões Lopes" "apellidos" => "Quintana" ] 3 => array:2 [ "nombre" => "Vanderlei Carneiro da" "apellidos" => "Silva" ] 4 => array:2 [ "nombre" => "Guilherme Carlos" "apellidos" => "Brech" ] 5 => array:2 [ "nombre" => "Lorena Gonçalves" "apellidos" => "Barbosa" ] 6 => array:2 [ "nombre" => "José Eduardo" "apellidos" => "Pompeu" ] 7 => array:2 [ "nombre" => "Erika Christina Gouveia e" "apellidos" => "Silva" ] 8 => array:2 [ "nombre" => "Elizabeth Mendes da" "apellidos" => "Silva" ] 9 => array:2 [ "nombre" => "Caroline Gil de" "apellidos" => "Godoy" ] 10 => array:2 [ "nombre" => "Julia Maria D'Andréa" "apellidos" => "Greve" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1807593222002617?idApp=UINPBA00004N" "url" => "/18075932/000000760000000C/v1_202211191031/S1807593222002617/v1_202211191031/en/main.assets" ] "en" => array:19 [ "idiomaDefecto" => true "cabecera" => "<span class="elsevierStyleTextfn">ORIGINAL ARTICLE</span>" "titulo" => "Extended minimally invasive autopsy: Technical improvements for the investigation of cardiopulmonary events in COVID-19" "tieneTextoCompleto" => true "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "Jair Theodoro-Filho, Renata Aparecida de Almeida Monteiro, Amaro Nunes Duarte-Neto, Thais Mauad, Luiz Fernando Ferraz da Silva, Paulo Hilário Nascimento Saldiva, Marisa Dolhnikoff" "autores" => array:7 [ 0 => array:2 [ "nombre" => "Jair" "apellidos" => "Theodoro-Filho" ] 1 => array:2 [ "nombre" => "Renata Aparecida de Almeida" "apellidos" => "Monteiro" ] 2 => array:2 [ "nombre" => "Amaro Nunes" "apellidos" => "Duarte-Neto" ] 3 => array:2 [ "nombre" => "Thais" "apellidos" => "Mauad" ] 4 => array:2 [ "nombre" => "Luiz Fernando Ferraz da" "apellidos" => "Silva" ] 5 => array:2 [ "nombre" => "Paulo Hilário Nascimento" "apellidos" => "Saldiva" ] 6 => array:4 [ "nombre" => "Marisa" "apellidos" => "Dolhnikoff" "email" => array:1 [ 0 => "maridol@usp.br" ] "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "*" "identificador" => "cor1" ] ] ] ] "afiliaciones" => array:1 [ 0 => array:2 [ "entidad" => "Laboratorio de Investigacao Medica (LIM-05), Departamento de Patologia, Faculdade de Medicina FMUSP, Universidade de Sao Paulo, Sao Paulo, SP, BR" "identificador" => "aff1" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "cor1" "etiqueta" => "*" "correspondencia" => "Corresponding author." ] ] ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig1" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 1179 "Ancho" => 1753 "Tamanyo" => 432948 ] ] "descripcion" => array:1 [ "en" => "<p id="spara10" class="elsevierStyleSimplePara elsevierViewall">Example of a non-COVID-19, preserved lung, fixed with gaseous formaldehyde at 30 cm H<span class="elsevierStyleInf">2</span>O for 48h. Lung parenchyma shows preserved architecture and normal histology, except for areas of anthracosis (black dots in upper left panel).</p>" ] ] ] "textoCompleto" => "<span class="elsevierStyleSections"><span id="cesec10" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle60">INTRODUCTION</span><p id="para10" class="elsevierStylePara elsevierViewall">Minimally invasive autopsy (MIA) is an alternative to conventional autopsies in some situations. In areas devoid of autopsy facilities, MIA is used to establish the cause of death, especially in the context of infectious conditions (<a class="elsevierStyleCrossRef" href="#bib1">1</a>,<a class="elsevierStyleCrossRef" href="#bib2">2</a>). The portability of MIA offers a unique opportunity to conduct autopsy in distant settings such as that in Brazil since the 1920s, a period in which a special device, the viscerotome, was employed in the investigation of the pathogenesis of yellow fever and other infectious diseases, such as leishmaniasis and schistosomiasis (<a class="elsevierStyleCrossRef" href="#bib3">3</a>).</p><p id="para20" class="elsevierStylePara elsevierViewall">Briefly, MIA involves obtaining tissue samples from different organs via percutaneous puncturing (<a class="elsevierStyleCrossRef" href="#bib1">1</a>,<a class="elsevierStyleCrossRef" href="#bib2">2</a>). It can be performed using external anatomical references or under image guidance. Imaging—magnetic resonance, computed tomography (CT), or ultrasound—increases the sensitivity of MIA (<a class="elsevierStyleCrossRef" href="#bib2">2</a>,<a class="elsevierStyleCrossRef" href="#bib4">4</a>). In our institution, ultrasound-guided MIA (MIA-US) has been employed since 2016 in selected cases (<a class="elsevierStyleCrossRef" href="#bib5">5</a>). When the COVID-19 pandemic emerged, MIA-US was the only option available to conduct autopsies, since conventional autopsies were interrupted owing to the risk of contagion. From the beginning of the pandemic, our team performed 252 MIA-US procedures; 117 of these patients died of COVID-19 (confirmed by RT-PCR and/or immunohistochemistry). The remaining 135 cases comprised 71 with other clinical conditions and 64 of intrauterine or neonatal deaths. The study of COVID-19 cases provided information on different aspects of the pathophysiology of the severe form of the disease, such as the characterization of pulmonary injury (<a class="elsevierStyleCrossRef" href="#bib6">6</a>), thrombogenic potential of COVID-19 (<a class="elsevierStyleCrossRef" href="#bib7">7</a>), timeline of pulmonary remodeling (<a class="elsevierStyleCrossRef" href="#bib8">8</a>), systemic distribution of the viral infection (<a class="elsevierStyleCrossRef" href="#bib6">6</a>,<a class="elsevierStyleCrossRef" href="#bib9">9</a>), and peculiar characteristics of severe COVID-19 in children (<a class="elsevierStyleCrossRef" href="#bib9">9</a>).</p><p id="para30" class="elsevierStylePara elsevierViewall">Owing to limitations of MIA-US for evaluating specific clinical conditions, such as cardiopulmonary events involving large vessels, during the pandemic, we continuously improved the technique to refine the investigation of thromboembolic events and myocardial infarction, important presentations and complications of severe COVID-19. We have developed an alternative approach, in which conventional percutaneous tissue sampling is combined with a small thoracic incision, allowing sampling of a larger amount of tissue than that in post-mortem biopsies, or even the removal of the entire organ. In the present report, we exemplify the usefulness of this extended MIA-US (EMIA-US) technique for the study of thoracic involvement in COVID-19.</p></span><span id="cesec20" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle70">METHODS</span><p id="para40" class="elsevierStylePara elsevierViewall">The images presented herein were obtained from autopsies of patients who died of COVID-19 at HC-FMUSP in 2021, in the city of Sao Paulo, Brazil. This protocol was approved by the HC-FMUSP Ethical Committee (protocol no. 3951.904). The procedures were performed in accordance with the ethical standards of the Ethical Committee on Human Experimentation and with the Helsinki Declaration.</p><p id="para50" class="elsevierStylePara elsevierViewall">The conventional MIA-US protocol is based on the percutaneous tissue sampling of several organs under ultrasound guidance (<a class="elsevierStyleCrossRef" href="#bib10">10</a>). Briefly, images obtained using a portable ultrasound (SonoSite M-Turbo R, Fujifilm, Bothell, WA, USA) were used to localize and orient sampling of several organs and to select the most affected areas within each organ. Tissue sampling was performed using Tru-Cut semi-automatic coaxial needles (14G; 20 cm in length) (<a class="elsevierStyleCrossRef" href="#bib6">6</a>,<a class="elsevierStyleCrossRef" href="#bib9">9</a>,<a class="elsevierStyleCrossRef" href="#bib10">10</a>). In EMIA-US, we complement the conventional MIA-US approach by performing a small (≤5 cm) thoracotomy between the left 3<span class="elsevierStyleSup">rd</span> and 4<span class="elsevierStyleSup">th</span> intercostal spaces. Subcutaneous tissue was separated from the rib cage, and two to three ribs were dissected and extracted from the costal cartilage up to 4 cm before reaching the subcostal groove. After opening the rib cage, the pericardium was opened, and the heart was exposed. Subsequently, the left mediastinal pleura was carefully sectioned to expose the left lung. The pulmonary hilum was exposed, and the main bronchus, pulmonary artery, and veins were sectioned 2 cm away from the heart, allowing the removal of the whole organ. The left lung was then gently pulled from the rib cage to be removed entirely. Using the same left incision and dissection procedures, the right lung was removed as well. The thorax was then filled with sawdust as in conventional autopsies, and a subcutaneous dermal suture was performed by approximating the two incision borders, and the skin was approximated and fixed with methacrylate glue.</p><p id="para60" class="elsevierStylePara elsevierViewall">The heart was sectioned to expose its chamber lumen and immersed in 10% buffered formalin solution for inspection the next day. One of the lungs (the lung with better preservation of the hilar structures) was fixed with gaseous formaldehyde for 2 consecutive days at 30 cm H<span class="elsevierStyleInf">2</span>O of transpulmonary pressure. The lung was then subjected to conventional CT (ex-situ CT). After macroscopic inspection and photographic documentation, representative samples were subjected to routine histological processing. The other lung was sampled in the autopsy room for molecular studies (samples stored at -80<span class="elsevierStyleSup">o</span>C), and then fixed with an intratracheal injection of formalin for 24h for conventional histological procedures.</p></span><span id="cesec30" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle80">RESULTS</span><p id="para70" class="elsevierStylePara elsevierViewall"><a class="elsevierStyleCrossRef" href="#fig1">Figure 1</a> shows a non-COVID-19 preserved lung, fixed with gaseous formaldehyde at 30 cm H<span class="elsevierStyleInf">2</span>O for 48h. EMIA-US was performed in eight patients who died of severe COVID-19. <a class="elsevierStyleCrossRefs" href="#fig2">Figures 2-6</a> show examples of cardiopulmonary events that could be evaluated using EMIA-US in these patients.</p><elsevierMultimedia ident="fig1"></elsevierMultimedia><elsevierMultimedia ident="fig2"></elsevierMultimedia><elsevierMultimedia ident="fig3"></elsevierMultimedia><elsevierMultimedia ident="fig4"></elsevierMultimedia><elsevierMultimedia ident="fig5"></elsevierMultimedia><elsevierMultimedia ident="fig6"></elsevierMultimedia></span><span id="cesec40" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle90">DISCUSSION</span><p id="para80" class="elsevierStylePara elsevierViewall">This study presents a descriptive report of a new technical development for conducting non-conventional clinical autopsies. EMIA-US combines transcutaneous image-guided post-mortem biopsies with reduced thoracotomy to investigate cardiopulmonary involvement in the disease. EMIA-US was created owing to the need to investigate thrombotic and ischemic cardiopulmonary events, which are important aspects of the pathogenesis of severe COVID-19. The set of images presented in this report illustrates the potential of this procedure.</p><p id="para90" class="elsevierStylePara elsevierViewall">EMIA-US can be safely conducted in spaces without a conventional autopsy facility. Bleeding is minimal and can be efficiently controlled using a simple surgical aspirator to reduce the risk of contagion. Thus, EMIA-US fulfills one of the main advantages of MIA, that is, the ability to conduct comprehensive post-mortem investigation of human diseases in areas or sites devoid of autopsy facilities.</p><p id="para100" class="elsevierStylePara elsevierViewall">The possibility of larger tissue sampling than that in post-mortem biopsies performed using conventional MIA-US was useful to provide a clearer picture of the magnitude and severity of COVID-19, as exemplified in <a class="elsevierStyleCrossRefs" href="#fig2">Figures 2-6</a>. In addition to the original objective—a better evaluation of thromboembolic events and myocardial infarction—EMIA-US was useful for identifying the magnitude of pulmonary damage induced by mechanical ventilation (<a class="elsevierStyleCrossRef" href="#fig6">Figure 6</a>) and the various aspects of COVID-19 progression (<a class="elsevierStyleCrossRef" href="#fig4">Figure 4</a>). While conducting EMIA-US, we improved the technical skills of our team up to the point of being able to extract the entire lung with intact pleural coverage, creating the possibility of performing lung fixation with formaldehyde vapor. Having an air-filled and fixed lung, we made it possible to obtain, to the best of our knowledge, the first ex-situ lung CT images of a patient with COVID-19 (<a class="elsevierStyleCrossRef" href="#fig5">Figure 5</a>). The possibility of combining ex-situ CT, macroscopic, and microscopic sets of images creates conditions to elaborate a pictorial atlas of lung diseases, designed to improve medical education.</p></span><span id="cesec50" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle100">CONCLUSIONS</span><p id="para110" class="elsevierStylePara elsevierViewall">Minimally invasive image-guided post-mortem tissue sampling is a flexible and practical method to conduct post-mortem studies of human diseases, mainly in areas devoid of autopsy facilities or, alternatively, when autopsy is not possible owing to financial constraints, cultural and religious values, or for safety reasons, as in the case of highly contagious infectious diseases. Because of its practicality and mobility, it can be expanded to other areas of Brazil with very scarce resources (central and northern regions of the country), expanding the tools for sanitary surveillance of high-impact infectious diseases. We present evidence that an Extended MIA-US technique is feasible and can be used as an alternative to increase the accuracy of MIA-US in detecting cardiopulmonary events involving large vessels, which may not be assessed through post-mortem biopsies. Notably, MIA may revive the practice of clinical-pathological grounds in medical schools, which is, in our best judgment, essential for solid medical education.</p></span><span id="cesec60" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle110">AUTHOR CONTRIBUTIONS</span><p id="para120" class="elsevierStylePara elsevierViewall">Theodoro-Filho J, Monteiro RAA and Saldiva PHN performed the autopsies. Duarte-Neto AN, Mauad T, Silva LFF, Saldiva PHN and Dolhnikoff M performed microscopic analysis and diagnostic interpretation. Saldiva PHN and Dolhnikoff M drafted the manuscript. All authors approved the final version of the manuscript. The authors participated sufficiently in the work to take public responsibility for appropriate portions of the content.</p></span></span>" "textoCompletoSecciones" => array:1 [ "secciones" => array:9 [ 0 => array:2 [ "identificador" => "xpalclavsec1577356" "titulo" => "KEYWORDS" ] 1 => array:2 [ "identificador" => "cesec10" "titulo" => "INTRODUCTION" ] 2 => array:2 [ "identificador" => "cesec20" "titulo" => "METHODS" ] 3 => array:2 [ "identificador" => "cesec30" "titulo" => "RESULTS" ] 4 => array:2 [ "identificador" => "cesec40" "titulo" => "DISCUSSION" ] 5 => array:2 [ "identificador" => "cesec50" "titulo" => "CONCLUSIONS" ] 6 => array:2 [ "identificador" => "cesec60" "titulo" => "AUTHOR CONTRIBUTIONS" ] 7 => array:2 [ "identificador" => "xack636832" "titulo" => "ACKNOWLEDGMENTS" ] 8 => array:1 [ "titulo" => "REFERENCES" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2021-09-29" "fechaAceptado" => "2021-10-20" "PalabrasClave" => array:1 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "KEYWORDS" "identificador" => "xpalclavsec1577356" "palabras" => array:3 [ 0 => "Autopsy" 1 => "Minimally Invasive Autopsy" 2 => "COVID-19" ] ] ] ] "tieneResumen" => true "resumen" => array:1 [ "en" => array:2 [ "resumen" => "<span id="ceabs10" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle10">OBJECTIVES:</span><p id="spara70" class="elsevierStyleSimplePara elsevierViewall">Ultrasound-guided minimally invasive autopsies (MIA-US) are an alternative to conventional autopsies and have been used in our institution to investigate the pathophysiology of COVID-19 since the beginning of the pandemic. Owing to the limitations of post-mortem biopsies for evaluating cardiopulmonary events involving large vessels, we continuously improved the technique during this period. Objectives: To demonstrate the usefulness of an extended MIA-US technique (EMIA-US) for the study of thoracic involvement in COVID-19.</p></span> <span id="ceabs20" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle20">METHOD:</span><p id="spara80" class="elsevierStyleSimplePara elsevierViewall">US-guided percutaneous tissue sampling was combined with a small thoracic incision (≤5 cm), allowing for the sampling of larger tissue samples or even the entire organ (lungs and heart).</p></span> <span id="ceabs30" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle30">RESULTS:</span><p id="spara90" class="elsevierStyleSimplePara elsevierViewall">EMIA-US was performed for eight patients who died of COVID-19 in 2021. We demonstrate cardiopulmonary events, mainly thromboembolism and myocardial infarction, that could be evaluated using EMIA-US.</p></span> <span id="ceabs40" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle40">CONCLUSIONS:</span><p id="spara100" class="elsevierStyleSimplePara elsevierViewall">Minimally invasive image-guided post-mortem tissue sampling is a flexible and practical method to conduct post-mortem studies of human diseases, mainly in areas that do not have autopsy facilities or, alternatively, when autopsy is not possible owing to financial constraints, cultural and religious values, or for safety reasons, such as in the case of highly contagious infectious diseases. We present evidence that EMIA-US is feasible and can be used as an alternative to increase the accuracy of MIA-US in detecting cardiopulmonary events involving large vessels, which may not be assessed through post-mortem biopsies.</p></span>" "secciones" => array:4 [ 0 => array:2 [ "identificador" => "ceabs10" "titulo" => "OBJECTIVES:" ] 1 => array:2 [ "identificador" => "ceabs20" "titulo" => "METHOD:" ] 2 => array:2 [ "identificador" => "ceabs30" "titulo" => "RESULTS:" ] 3 => array:2 [ "identificador" => "ceabs40" "titulo" => "CONCLUSIONS:" ] ] ] ] "NotaPie" => array:1 [ 0 => array:1 [ "nota" => "<p class="elsevierStyleNotepara" id="cenpara10">No potential conflict of interest was reported.</p>" ] ] "multimedia" => array:6 [ 0 => array:7 [ "identificador" => "fig1" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 1179 "Ancho" => 1753 "Tamanyo" => 432948 ] ] "descripcion" => array:1 [ "en" => "<p id="spara10" class="elsevierStyleSimplePara elsevierViewall">Example of a non-COVID-19, preserved lung, fixed with gaseous formaldehyde at 30 cm H<span class="elsevierStyleInf">2</span>O for 48h. Lung parenchyma shows preserved architecture and normal histology, except for areas of anthracosis (black dots in upper left panel).</p>" ] ] 1 => array:7 [ "identificador" => "fig2" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 1661 "Ancho" => 1753 "Tamanyo" => 459391 ] ] "descripcion" => array:1 [ "en" => "<p id="spara20" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleBold">A</span> and <span class="elsevierStyleBold">B</span>: Male, 56 years old, 30 days of hospitalization. <span class="elsevierStyleBold">A</span>: Cross section of the heart at the atrium-ventricle transition. Bottom view of an organized thrombus in the right atrium (arrow). <span class="elsevierStyleBold">B</span>: Upper view of the organized thrombus in the right atrium (arrow). <span class="elsevierStyleBold">C</span> and <span class="elsevierStyleBold">D</span>: Male, 76 years old, 22 days of hospitalization. Gross section <span class="elsevierStyleBold">(C)</span> and histology <span class="elsevierStyleBold">(D)</span> of the left ventricle showing scarring areas of ischemic heart disease (arrow in <span class="elsevierStyleBold">C</span> and asterisk in <span class="elsevierStyleBold">D</span>).</p>" ] ] 2 => array:7 [ "identificador" => "fig3" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 1202 "Ancho" => 1621 "Tamanyo" => 430080 ] ] "descripcion" => array:1 [ "en" => "<p id="spara30" class="elsevierStyleSimplePara elsevierViewall"><span class="elsevierStyleBold">A</span> and <span class="elsevierStyleBold">B</span>: Male, 89 years old, 7 days of hospitalization. Gross section <span class="elsevierStyleBold">(A)</span> and histology <span class="elsevierStyleBold">(B)</span> of thromboembolism in a medium-sized (3.0 mm in diameter) pulmonary artery (arrow in <span class="elsevierStyleBold">A</span> and <span class="elsevierStyleBold">B</span>). <span class="elsevierStyleBold">C</span>: Male, 76 years old, 24 days of hospitalization. Same patient as in <a class="elsevierStyleCrossRef" href="#fig2">Figure <span class="elsevierStyleBold">2C and 2D</span></a>. Thromboembolism in a medium-sized (4.0 mm in diameter) pulmonary artery (arrow).</p>" ] ] 3 => array:7 [ "identificador" => "fig4" "etiqueta" => "Figure 4" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr4.jpeg" "Alto" => 1052 "Ancho" => 1663 "Tamanyo" => 448836 ] ] "descripcion" => array:1 [ "en" => "<p id="spara40" class="elsevierStyleSimplePara elsevierViewall">Female, 42 years old, 38 days of hospitalization and mechanical ventilation. <span class="elsevierStyleBold">A</span>: Left inferior lung lobe shows marked transition between upper and lower regions, representing two different patterns of lung injury. Upper region corresponds to acute lung injury and lower region to advanced lung remodeling with fibrosis. <span class="elsevierStyleBold">B</span>: Acute lung injury, with predominance of exudative changes, corresponds to the upper left inferior lobe shown in <span class="elsevierStyleBold">A</span>. <span class="elsevierStyleBold">C</span>: Remodeling of lung parenchyma with extensive fibrosis corresponds to the lower left inferior lobe shown in <span class="elsevierStyleBold">A</span>.</p>" ] ] 4 => array:7 [ "identificador" => "fig5" "etiqueta" => "Figure 5" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr5.jpeg" "Alto" => 1666 "Ancho" => 1753 "Tamanyo" => 358380 ] ] "descripcion" => array:1 [ "en" => "<p id="spara50" class="elsevierStyleSimplePara elsevierViewall">Male, 89 years old, 7 days of hospitalization, same patient as in <a class="elsevierStyleCrossRef" href="#fig3">Figure <span class="elsevierStyleBold">3A</span> and <span class="elsevierStyleBold">3B</span></a>. Ex-situ computed tomography <span class="elsevierStyleBold">(B)</span> showing perfect correlation with the macroscopic evaluation of the lung <span class="elsevierStyleBold">(A)</span>, ground-glass opacities, peripheral reticular pattern, and an area of lung cavitation (arrow in <span class="elsevierStyleBold">A</span> and <span class="elsevierStyleBold">B</span>), likely resulting from a previous abscess, with fistula formation toward the pleura.</p>" ] ] 5 => array:7 [ "identificador" => "fig6" "etiqueta" => "Figure 6" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr6.jpeg" "Alto" => 2454 "Ancho" => 1654 "Tamanyo" => 864108 ] ] "descripcion" => array:1 [ "en" => "<p id="spara60" class="elsevierStyleSimplePara elsevierViewall">Female, 17 years old, 20 days of hospitalization, 16 days of mechanical ventilation. Bilateral pneumothorax and subcutaneous emphysema in the chest and face reported 9 days before death. <span class="elsevierStyleBold">A</span>: Multiple areas of pulmonary interstitial emphysema secondary to barotrauma. Note a ruptured subpleural bubble (arrow). <span class="elsevierStyleBold">B</span>: Pulmonary interstitial emphysema; air dissects along connective tissue sheaths of the bronchovascular bundles and interlobular septa. Arrow: arteriolar thrombus.</p>" ] ] ] "bibliografia" => array:2 [ "titulo" => "REFERENCES" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "cebibsec10" "bibliografiaReferencia" => array:10 [ 0 => array:3 [ "identificador" => "bib1" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Needle autopsy to establish the cause of death in HIV-infected hospitalized adults in Uganda: a comparison to complete autopsy" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ JA Cox \n \t\t\t\t\t\t\t\t """ 1 => """ RL Lukande \n \t\t\t\t\t\t\t\t """ 2 => """ S Kalungi \n \t\t\t\t\t\t\t\t """ 3 => """ E Van Marck \n \t\t\t\t\t\t\t\t """ 4 => """ K Van de Vijver \n \t\t\t\t\t\t\t\t """ 5 => """ A Kambugu \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1097/QAI.0000000000000290" "Revista" => array:7 [ "tituloSerie" => "J Acquir Immune Defic Syndr" "fecha" => "2014" 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] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1186/s12931-021-01628-9" "Revista" => array:6 [ "tituloSerie" => "Respir Res" "fecha" => "2021" "volumen" => "22" "numero" => "1" "paginaInicial" => "32" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/33514373" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1186/s12931-021-01628-9" "WWW" => array:1 [ "link" => "https://doi.org/10.1186/s12931-021-01628-9" ] ] ] ] ] ] 8 => array:3 [ "identificador" => "bib9" "etiqueta" => "9" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "An autopsy study of the spectrum of severe COVID-19 in children: From SARS to different phenotypes of MIS-C" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ AN Duarte-Neto \n \t\t\t\t\t\t\t\t """ 1 => """ EG Caldini \n \t\t\t\t\t\t\t\t """ 2 => """ MS Gomes-Gouvêa \n \t\t\t\t\t\t\t\t """ 3 => """ CT Kanamura \n \t\t\t\t\t\t\t\t """ 4 => """ RA de Almeida Monteiro \n \t\t\t\t\t\t\t\t """ 5 => """ JF Ferranti \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:1 [ "Revista" => array:3 [ "tituloSerie" => "EClinicalMedicine" "fecha" => "2021" "volumen" => "35" ] ] 1 => array:1 [ "WWW" => array:1 [ "link" => "https://doi.org/10.1016/j.eclinm.2021.100850" ] ] ] ] ] ] 9 => array:3 [ "identificador" => "bib10" "etiqueta" => "10" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Ultrasound-guided minimally invasive autopsies: A protocol for the study of pulmonary and systemic involvement of COVID-19" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ RAA Monteiro \n \t\t\t\t\t\t\t\t """ 1 => """ AN Duarte-Neto \n \t\t\t\t\t\t\t\t """ 2 => """ LFFD Silva \n \t\t\t\t\t\t\t\t """ 3 => """ EP Oliveira \n \t\t\t\t\t\t\t\t """ 4 => """ JT Filho \n \t\t\t\t\t\t\t\t """ 5 => """ GABD Santos \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:1 [ "Revista" => array:4 [ "tituloSerie" => "Clinics (Sao Paulo)" "fecha" => "2020" "volumen" => "75" "itemHostRev" => array:3 [ "pii" => "S0015028207043075" "estado" => "S300" "issn" => "00150282" ] ] ] 1 => array:1 [ "WWW" => array:1 [ "link" => "https://doi.org/10.6061/clinics/2020/e1972" ] ] ] ] ] ] ] ] ] ] "agradecimientos" => array:1 [ 0 => array:4 [ "identificador" => "xack636832" "titulo" => "ACKNOWLEDGMENTS" "texto" => "<p id="para130" class="elsevierStylePara elsevierViewall">The authors would like to thank Reginaldo Silva do Nascimento, Kely Cristina Soares Bispo, and Cássia Arruda for their technical support. We are also grateful to all healthcare workers involved in COVID-19 patient care; to everyone at the Coronavirus Crisis Committee of our institution; and to all legal representatives who agreed to the post-mortem examination of their relatives. Funding: Bill and Melinda Gates Foundation [INV-002396]; Conselho Nacional de Desenvolvimento Científico e Tecnológico [401825/2020-5, 304987/2017-4 for MD and 304277/2019-3 for TM]; Fundação de Amparo è Pesquisa do Estado de São Paulo 2013/17159-2; Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo - HC Convida [HC-02.18/2020 and HC-01.29/2020].</p>" "vista" => "all" ] ] ] "idiomaDefecto" => "en" "url" => "/18075932/000000760000000C/v1_202211191031/S1807593222002629/v1_202211191031/en/main.assets" "Apartado" => null "PDF" => "https://static.elsevier.es/multimedia/18075932/000000760000000C/v1_202211191031/S1807593222002629/v1_202211191031/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1807593222002629?idApp=UINPBA00004N" ]
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