Triple-negative breast carcinomas are a heterogeneous entity that differs between young and old patients

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The interval between the administration of ketamin until the loss of the righting reflex was recorded as onset of sleep. The time from loss of righting reflex to regaining of the righting reflex was recorded as duration of sleep. Results represent mean (± S.E.M.) from six mice. ∗P < 0.01 compared with vehicle-treated control." ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Valiollah Hajhashemi, Mohammad Rabbani, Alireza Ghanadi, Elahe Davari" "autores" => array:4 [ 0 => array:2 [ "nombre" => "Valiollah" "apellidos" => "Hajhashemi" ] 1 => array:2 [ "nombre" => "Mohammad" "apellidos" => "Rabbani" ] 2 => array:2 [ "nombre" => "Alireza" "apellidos" => "Ghanadi" ] 3 => array:2 [ "nombre" => "Elahe" "apellidos" => "Davari" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1807593222029325?idApp=UINPBA00004N" "url" => "/18075932/0000006500000010/v1_202212010724/S1807593222029325/v1_202212010724/en/main.assets" ] "itemAnterior" => array:19 [ "pii" => "S1807593222029301" "issn" => "18075932" "doi" => "10.1590/S1807-59322010001000018" "estado" => "S300" "fechaPublicacion" => "2010-01-01" "aid" => "2930" "copyright" => "CLINICS" "documento" => "article" "crossmark" => 0 "licencia" => "https://creativecommons.org/licenses/by-nc/3.0/" "subdocumento" => "fla" "cita" => "Clinics. 2010;65:1027-32" "abierto" => array:3 [ "ES" => true "ES2" => true "LATM" => true ] "gratuito" => true "lecturas" => array:1 [ "total" => 0 ] "en" => array:12 [ "idiomaDefecto" => true "cabecera" => "Clinical Science" "titulo" => "Increased frequency of anti-retina antibodies in asymptomatic patients with chronic t. gondii infection" "tienePdf" => "en" "tieneTextoCompleto" => "en" "tieneResumen" => "en" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "1027" "paginaFinal" => "1032" ] ] "contieneResumen" => array:1 [ "en" => true ] "contieneTextoCompleto" => array:1 [ "en" => true ] "contienePdf" => array:1 [ "en" => true ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig3-cln_65p1027" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr3.jpeg" "Alto" => 395 "Ancho" => 442 "Tamanyo" => 19875 ] ] "descripcion" => array:1 [ "en" => "Antibodies against bovine interphotoreceptor retinoid-binding protein (IRBP) antigen in selected patients. Non-Tg uveitis: samples from patients with uveitis other than toxoplasmosis (n = 50; see Methods). Tg uveitis: samples from Toxoplasma uveitis patients (n = 30). Tg IgG-: samples from patients who were seronegative for T. gondii (n = 250). Tg IgG+: samples from patients who were seropositive for T. gondii. Bars represent mean values. The y-axis was interrupted at cut-off using the mean minus 2 SD of samples from all uveitis patients." ] ] ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Sylvia Regina Temer Cursino, Thaís Boccia da Costa, Joyce Hisae Yamamoto, Luciana Regina Meireles, Maria Antonieta Longo Galvão Silva, Heitor Franco de Andrade Junior" "autores" => array:6 [ 0 => array:2 [ "nombre" => "Sylvia Regina Temer" "apellidos" => "Cursino" ] 1 => array:2 [ "nombre" => "Thaís Boccia" "apellidos" => "da Costa" ] 2 => array:2 [ "nombre" => "Joyce Hisae" "apellidos" => "Yamamoto" ] 3 => array:2 [ "nombre" => "Luciana Regina" "apellidos" => "Meireles" ] 4 => array:2 [ "nombre" => "Maria Antonieta Longo Galvão" "apellidos" => "Silva" ] 5 => array:2 [ "nombre" => "Heitor Franco" "apellidos" => "de Andrade Junior" ] ] ] ] ] "idiomaDefecto" => "en" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1807593222029301?idApp=UINPBA00004N" "url" => "/18075932/0000006500000010/v1_202212010724/S1807593222029301/v1_202212010724/en/main.assets" ] "en" => array:19 [ "idiomaDefecto" => true "cabecera" => "Clinical Science" "titulo" => "Triple-negative breast carcinomas are a heterogeneous entity that differs between young and old patients" "tieneTextoCompleto" => true "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "1033" "paginaFinal" => "1036" ] ] "autores" => array:1 [ 0 => array:4 [ "autoresLista" => "Filomena M Carvalho, Livia M Bacchi, Priscila P C Santos, Carlos E Bacchi" "autores" => array:4 [ 0 => array:4 [ "nombre" => "Filomena M" "apellidos" => "Carvalho" "email" => array:1 [ 0 => "filomena@usp.br" ] "referencia" => array:2 [ 0 => array:2 [ "etiqueta" => "I" "identificador" => "af1-cln_65p1033" ] 1 => array:2 [ "etiqueta" => "*" "identificador" => "c1-cln_65p1033" ] ] ] 1 => array:3 [ "nombre" => "Livia M" "apellidos" => "Bacchi" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "I" "identificador" => "af1-cln_65p1033" ] ] ] 2 => array:3 [ "nombre" => "Priscila P C" "apellidos" => "Santos" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "I" "identificador" => "af1-cln_65p1033" ] ] ] 3 => array:3 [ "nombre" => "Carlos E" "apellidos" => "Bacchi" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "II" "identificador" => "af2-cln_65p1033" ] ] ] ] "afiliaciones" => array:2 [ 0 => array:3 [ "entidad" => "Department of Pathology, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil" "etiqueta" => "I" "identificador" => "af1-cln_65p1033" ] 1 => array:3 [ "entidad" => "Consultoria em Patologia, Botucatu, SP, Brazil" "etiqueta" => "II" "identificador" => "af2-cln_65p1033" ] ] "correspondencia" => array:1 [ 0 => array:3 [ "identificador" => "c1-cln_65p1033" "etiqueta" => "*" "correspondencia" => "Tel.: 55 11 3061-7254" ] ] ] ] "resumenGrafico" => array:2 [ "original" => 0 "multimedia" => array:7 [ "identificador" => "fig1-cln_65p1033" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 340 "Ancho" => 498 "Tamanyo" => 61741 ] ] "descripcion" => array:1 [ "en" => "A high-grade invasive ductal carcinoma (A) with intense, diffuse CK5/6 immunoexpression (B)." ] ] ] "textoCompleto" => "INTRODUCTIONBreast cancer detected in younger patients has been associated with a more aggressive phenotype.<a class="elsevierStyleCrossRefs" href="#bib1">1-3</a> To improve treatment outcomes and to reduce mortality from this disease, a greater understanding of this aggressive phenotype is needed. Gene-expression profiling using DNA microarrays has identified five subtypes of breast cancer (i.e., luminal A, luminal B, normal breast-like, HER2-overexpression, and basal-like). Each of these subtypes is associated with a distinct prognosis, but the different subtypes share similarities. For example, immunohistochemistry has been used to evaluate the expression of estrogen receptors (ERs), progesterone receptors (PRs), HER2, and Ki-67 to characterize the different subtypes.<a class="elsevierStyleCrossRef" href="#bib4">4</a> The basal-like and HER2+ subtypes both have a shorter relapse-free survival and overall survival than luminal tumors.<a class="elsevierStyleCrossRefs" href="#bib5">5,6</a> Triple-negative (TN) breast carcinomas (i.e., ER-negative, PR-negative, and HER2-negative tumors) have been shown to be related to a basal-like phenotype and, accordingly, exhibit more aggressive clinical and pathologic features.<a class="elsevierStyleCrossRef" href="#bib7">7</a> Triple-negative carcinomas are also more prevalent among specific subgroups of women, particularly younger patients, and have been found to be associated with BRCA1 germline mutations.<a class="elsevierStyleCrossRefs" href="#bib8">8-11</a> Although significant overlap in criteria of classification of TN, basal-like carcinomas, and BRCA1-related tumors has been shown, these three tumor types are not synonymous. Therefore, no consensus exists regarding the criteria that identify basal-like subgroups of TN carcinomas. Currently, the most useful criteria have been the expression of basal-cytokeratin (CK5/6) and/or epidermal growth factor receptor (EGFR);<a class="elsevierStyleCrossRef" href="#bib12">12</a> however, recent studies have indicated that TN carcinomas are not as homogeneous as they were first thought to be.<a class="elsevierStyleCrossRefs" href="#bib7">7,13,14</a> In this study, the frequencies and phenotypic characteristics of TN and basal-like carcinomas in patients who were 35 years old or younger were analyzed and compared with those of TN carcinomas diagnosed in patients who were 60 years old and older.METHODS AND MATERIALSThis project was approved by the Scientific Committee of the Department of Pathology of the Faculdade de Medicina da Universidade de Sao Paulo and by the Ethical Committee for Research Projects of the Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo (CAPPesq) (protocol 563/07). Between 1997 and 2007, 213 tumor specimens from patients who were 35 years old or younger were registered at the Consultoria em Patologia, a large reference laboratory located in Botucatu, Sao Paulo, Brazil. An additional 117 tumor samples from patients who were 60 years old or older were also analyzed by the same laboratory in 2006. All tumor samples from the two age groups were reviewed and classified according to histological type, histological grade, nuclear grade, the presence of tumor necrosis, the presence of an in situ component, and any vascular involvement.For each tumor, representative areas were selected to construct tissue microarrays (TMAs). Briefly, three cylinders, each 1.5 mm in diameter, were removed from selected areas of donor blocks and mounted into paraffin blocks at 1-mm intervals using a precision microarray instrument (Beecher Instruments, Silver Spring, MD). A grid system was established, and each core had a coordinate reference (i.e., x-axis, y-axis) for sample identification. Blocks were sealed at 60°C for 10 min, and 5-µm sections of the resulting TMA blocks were prepared using standard techniques and mounted on Starfrost® slides.Histological sections from the TMA blocks were immunostained for ER, PR, HER2, Ki-67 antigen, EGFR, CK5/6, p53, vimentin, CD117, and p63. The sources and dilutions of the antibodies and the epitope retrieval methods that were used are listed in <a class="elsevierStyleCrossRef" href="#t1-cln_65p1033">Table 1</a>. Bound antibodies were detected using Novolink system (Leica, USA).<elsevierMultimedia ident="t1-cln_65p1033"></elsevierMultimedia>Positive immunohistochemistry staining was used to identify cytoplasmic localization of CK5/6, vimentin, and CD117; nuclear localization of ER, PR, Ki-67, p63, and p53; and membranous-pattern staining of EGFR and HER2. In this study, only cases that had no ER- or PR-positive cells, were negative for HER2, and were scored as 0 or 1+ according to the guidelines of the American Society of Clinical Oncology (ASCO) and College of American Pathologists (CAP)<a class="elsevierStyleCrossRef" href="#bib15">15</a> were included. Ki-67 expression was scored as either < 25% or > 25%. For the other markers, at least 1% of the positive cells with a moderate to strong intensity were considered positive.Statistical analysis was performed using a two-way contingency table and the chi-square test.RESULTSTriple-negative breast carcinomas were diagnosed in 74/213 (34.7%) patients who were 35 years old or younger and in 19/117 (16.2%) patients who were 60 years old or older (p = 0.002). The median ages of the younger and older patients who were included in the study were 32 years (mean ± SD: 30.9 ± 3.94; range: 21-35 years) and 73 years (mean ± SD: 73.7 ± 7.53; range: 61-91 years), respectively. In the younger group, 67/74 (90.5%) tumors were of a ductal histological type; other histological types identified included metaplastic (2 cases), pleomorphic lobular (2 cases), secretory (1 case), mucinous (1 case), and medullary (1 case). Regarding the older patients, 15/19 (78.9%) tumors were of a ductal histological type, and 4 (21.1%) were of a pleomorphic lobular type. The pathological features of the tumors that were identified in each patient group are summarized in <a class="elsevierStyleCrossRef" href="#t2-cln_65p1033">Table 2</a>. The histological and nuclear grades and the angiovascular involvement were not different between the two patient groups. Tumoral necrosis was more frequently observed in tumors that were derived from younger patients than in those from older patients (52.7% vs. 31.6%, respectively). However, the difference was not statistically significant. The basal phenotype, which was determined by the expression of EGFR and/or CK5/6 according to the criteria of Nielsen et al.,<a class="elsevierStyleCrossRef" href="#bib12">12</a> was similar for both groups (74.3% for younger patients vs. 68.4% for older patients). However, the two markers showed differences in the frequency between the two groups (<a class="elsevierStyleCrossRefs" href="#fig1-cln_65p1033">Figures 1 and 2</a>). Tumors that were derived from the younger patients were more frequently EGFR positive (71.6% vs. 47.3%, respectively) and exhibited a lower frequency of CK5/6 expression (9.4% vs. 42.1%, respectively). Regarding the basal-like tumors of younger patients, 53/55 (96.4%) were associated with EGFR expression, whereas in the older patient group, 9/13 (69.2%) expressed EGFR (p = 0.0018). c-KIT expression was also observed more frequently in tumors that occurred in older patients (36.8%) than in younger patients (21.2%), although the difference was not significant. The analysis of other markers, including Ki-67, p63, p53, and vimentin, did not show any significant differences between the two groups.<elsevierMultimedia ident="t2-cln_65p1033"></elsevierMultimedia><elsevierMultimedia ident="fig1-cln_65p1033"></elsevierMultimedia><elsevierMultimedia ident="fig2-cln_65p1033"></elsevierMultimedia>DISCUSSIONBreast cancer that is detected in younger women is typically associated with aggressive behavior and results in a poor prognosis. Unfortunately, the specific mechanisms responsible for this tumor phenotype remain unclear. This poor understanding is further complicated by the controversial results of many studies. Currently, breast cancers that are diagnosed in younger patients are characterized by reduced hormone sensitivity and high HER2/EGFR expression,<a class="elsevierStyleCrossRefs" href="#bib2">2,16</a> although ER-positive carcinomas are still a prevalent subgroup of tumors in this age group.<a class="elsevierStyleCrossRefs" href="#bib1">1,16</a> Furthermore, luminal carcinomas in younger women are mostly subtype B, which is determined by their frequent co-expression of HER2 and/or their high proliferative activity.<a class="elsevierStyleCrossRef" href="#bib16">16</a> Triple-negative carcinomas tend to occur less frequently than luminal carcinomas; however, triple-negative carcinomas are more prevalent in patients who are younger than 35 years old.<a class="elsevierStyleCrossRef" href="#bib17">17</a>TN carcinomas are considered to be a group of biologically distinct neoplasias that mostly exhibit a basal phenotype and an aggressive biology. Unlike the other subtypes, targeted agents that are specifically aimed at triple-negative breast tumors are not yet available; thus, this deficiency intensifies the need and interest in advancing novel therapeutic strategies beyond chemotherapy for this subset of high-risk patients. Triple-negative is a term based on clinical assays for ER, PR, and HER2, whereas basal-like is a molecular phenotype that was initially defined using cDNA microarrays. However, low-grade tumors, such as adenoid cystic and medullary carcinomas, are also considered TN carcinomas.<a class="elsevierStyleCrossRefs" href="#bib13">13,18</a> Therefore, the best criteria to characterize the phenotype of basal carcinomas have remained somewhat controversial. Nielsen et al. proposed that the best immunohistochemistry predictors of basal-like gene expression are the positive expression of CK 5/6 and EGFR and the negative expression of HER2 and ER; these criteria were associated with a sensitivity of 76% and a specificity 100%.<a class="elsevierStyleCrossRef" href="#bib12">12</a> In this study, we analyzed these markers; however, it should be noted that other studies have considered the expression patterns of CK5, CK14, CK 17, p-cadherin, p63, c-kit, and vimentin to characterize a basal-like phenotype.<a class="elsevierStyleCrossRefs" href="#bib19">19-22</a> Differences in the molecular profile of basal-like carcinomas have also been associated with protein expression patterns of physiological stem/progenitor cells during breast development.<a class="elsevierStyleCrossRef" href="#bib23">23</a> In this histogenetic model, adult CK5-positive stem cells are hypothesized to differentiate into neoplastic stem cells as a result of EGFR amplification, a mutation of p53, or BRCA inactivation, and this differentiation leads to the generation of basal-like carcinomas. In general, these types of tumors express high levels of p-cadherin, EGFR, and CK5 and an absence or low levels of luminal markers, such as CK 8/18.<a class="elsevierStyleCrossRef" href="#bib23">23</a> Given the number of markers involved, the possibility that different expression profiles could define distinct subsets of basal-like carcinomas should be considered. For example, Rakha et al. compared tumors with a basal phenotype that were characterized by the expression of basal cytokeratins (CK5/6 or CK14) with cells with a myoepithelial phenotype (p63 or smooth muscle actin).<a class="elsevierStyleCrossRef" href="#bib24">24</a> The basal phenotype was associated with a worse outcome, which suggested that although basal and myoepithelial phenotypes share many features, including a similar genetic profile, they possible are distinct tumors.<a class="elsevierStyleCrossRef" href="#bib24">24</a>Very few studies exist that have examined molecular differences in breast tumors according to age.<a class="elsevierStyleCrossRefs" href="#bib1">1-3,9,16,25</a> In the present study, a higher prevalence of TN carcinoma was observed in younger patients (34.3% for younger patients vs. 16.3% for older patients), and a basal-like phenotype associated with positive expression of CK5/6 and/or EGFR was present in 74.3% vs. 68.4%, respectively. More importantly, the molecular profiles of the TN carcinomas from younger patients were different from the profiles of the tumors from older patients. Triple negative carcinomas in the younger age group were more frequently EGFR positive (71.6% vs. 47.3%, respectively) and less frequently CK5/6 positive (9.4% vs. 42.1%, respectively). The role of EGFR expression in breast cancer has been investigated by many authors.<a class="elsevierStyleCrossRefs" href="#bib11">11,14,26,27</a> Viale et al. reported a worse prognosis for patients with TN carcinomas that had EGFR-immunoreactivity present in more than 50% of neoplastic cells, which suggested a possible prognostic role for quantification.<a class="elsevierStyleCrossRef" href="#bib14">14</a> Similarly, Rimawi et al. used radioligand-binding assays to quantify EGFR expression in frozen sections of breast tissue carcinomas and found that higher levels of EGFR were associated with younger and black women, a more aggressive outcome, and lower levels of hormone receptors.<a class="elsevierStyleCrossRef" href="#bib27">27</a> The relationship between EGFR and BRCA1 has been investigated by different groups, and BRCA1-germline mutations have been associated with a basal-like phenotype and younger age.<a class="elsevierStyleCrossRefs" href="#bib8">8-11</a> In a study by Collins et al. that analyzed BRCA1 in 144 TN carcinomas, independent of the patient's age,<a class="elsevierStyleCrossRef" href="#bib26">26</a> they found a higher prevalence of basal CK and EGFR among TN breast cancers; however, the frequency of expression of these markers was similar in women with and without BRCA1 mutations.<a class="elsevierStyleCrossRef" href="#bib26">26</a> In contrast, Arnes et al. found that EGFR was a predictor of BRCA1 status, followed by patient age and ER status.<a class="elsevierStyleCrossRef" href="#bib11">11</a> In the same study, a worse prognosis was associated with a mutation of BRCA1 and positive expression of EGFR.<a class="elsevierStyleCrossRef" href="#bib11">11</a>As a receptor tyrosine kinase that plays essential roles in both normal physiological conditions and cancerous conditions, EGFR can affect many important characteristics of a cancer's phenotype, including evasion of apoptosis, proliferation, invasion, and metastasis. In a previous study, the frequency of EGFR-positive tumors among young patients was found to be higher than among older women (5.9% vs. 3.3%, respectively), although the difference was not statistically significant.<a class="elsevierStyleCrossRef" href="#bib16">16</a> Based on the results of that study and the data presented in this report, EGFR expression appears to play an important role in the early onset of breast cancer, and we hypothesize that this role is related to intrinsic genetic differences that result in an adverse outcome that is associated with the aggressive breast carcinomas. Furthermore, we propose that EGFR immunostaining and/or amplification should be further investigated as predictor of a patient's response to targeted therapy." "textoCompletoSecciones" => array:1 [ "secciones" => array:7 [ 0 => array:2 [ "identificador" => "xpalclavsec1581289" "titulo" => "KEYWORDS" ] 1 => array:2 [ "identificador" => "cesec10" "titulo" => "INTRODUCTION" ] 2 => array:2 [ "identificador" => "cesec20" "titulo" => "METHODS AND MATERIALS" ] 3 => array:2 [ "identificador" => "cesec30" "titulo" => "RESULTS" ] 4 => array:2 [ "identificador" => "cesec40" "titulo" => "DISCUSSION" ] 5 => array:2 [ "identificador" => "xack638827" "titulo" => "ACKNOWLEDGEMENTS" ] 6 => array:1 [ "titulo" => "REFERENCES" ] ] ] "pdfFichero" => "main.pdf" "tienePdf" => true "fechaRecibido" => "2010-09-15" "fechaAceptado" => "2010-09-20" "PalabrasClave" => array:1 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "KEYWORDS" "identificador" => "xpalclavsec1581289" "palabras" => array:5 [ 0 => "Breast cancer" 1 => "Triple-negative carcinomas" 2 => "Basal-like Carcinomas" 3 => "Young women" 4 => "Immunohistochemistry" ] ] ] ] "tieneResumen" => true "resumen" => array:1 [ "en" => array:2 [ "resumen" => "OBJECTIVE:To compare the frequency and immunohistochemical profiles of triple-negative breast carcinomas in younger and older women. METHODS AND RESULTS:We selected patients diagnosed with triple-negative breast carcinomas. The groups examined were women who were 35 years old or younger between 1997 and 2007 (n = 74) and, for comparison, women who were 60 years old or older (n = 19, consecutive cases). All formalin-fixed and paraffin-embedded tumor samples were reviewed and immunohistochemically stained for ER, PR, HER2, Ki-67 antigen, epidermal growth factor receptor, cytokeratin 5/6, p53, vimentin, CD117, and p63 using tissue microarrays blocks. Triple-negative breast carcinomas corresponded to 34.6% (74/213) of the carcinomas from the younger patients and 16.2% (19/117) of the carcinomas from the older patients (p = 0.002). No significant differences in the frequency of the basal phenotype were observed in the two patient groups based on CK5/6 and/or epidermal growth factor receptor expression (74.3% vs. 68.4%). However, triple-negative breast carcinomas in the older patients presented a higher frequency of CK5/6 expression compared to those of younger patients (42.1% vs. 9.6%; p = 0.005), whereas triple-negative breast carcinomas of younger patients had a higher expression level of epidermal growth factor receptor (71.6% vs. 47.3%). CONCLUSIONS:These results show that there were significant molecular differences between the triple-negative basal-like breast carcinomas that were diagnosed in younger women and those that were diagnosed in older women. These findings may provide a basis for describing the more aggressive phenotype of the triple-negative breast carcinomas observed in younger women." "secciones" => array:3 [ 0 => array:2 [ "identificador" => "ceabs10" "titulo" => "OBJECTIVE:" ] 1 => array:2 [ "identificador" => "ceabs20" "titulo" => "METHODS AND RESULTS:" ] 2 => array:2 [ "identificador" => "ceabs30" "titulo" => "CONCLUSIONS:" ] ] ] ] "multimedia" => array:4 [ 0 => array:7 [ "identificador" => "fig1-cln_65p1033" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr1.jpeg" "Alto" => 340 "Ancho" => 498 "Tamanyo" => 61741 ] ] "descripcion" => array:1 [ "en" => "A high-grade invasive ductal carcinoma (A) with intense, diffuse CK5/6 immunoexpression (B)." ] ] 1 => array:7 [ "identificador" => "fig2-cln_65p1033" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIAFIGURA" "mostrarFloat" => true "mostrarDisplay" => false "figura" => array:1 [ 0 => array:4 [ "imagen" => "gr2.jpeg" "Alto" => 332 "Ancho" => 498 "Tamanyo" => 61916 ] ] "descripcion" => array:1 [ "en" => "A high-grade invasive ductal carcinoma with numerous mitotic figures (A) and EGFR immunoexpression (B)." ] ] 2 => array:7 [ "identificador" => "t1-cln_65p1033" "etiqueta" => "Table 1" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "tabla" => array:2 [ "leyenda" => "ER: Estrogen receptor; PR: Progesterone receptor; EGFR: Epidermal growth factor receptor; CK: cytokeratinPressure cooker: citrate buffer (pH 6) (Tender Cooker, Nordic Wave, USA)Microwave: citrate buffer (pH 6), 15 min (Eletrolux, 900 W)" "tablatextoimagen" => array:1 [ 0 => array:1 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="bottom" scope="col" style="border-bottom: 2px solid black">Antigen \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="center" valign="bottom" scope="col" style="border-bottom: 2px solid black">Clone/Source \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="center" valign="bottom" scope="col" style="border-bottom: 2px solid black">Dilution \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="center" valign="bottom" scope="col" style="border-bottom: 2px solid black">Epitope retrieval method \t\t\t\t\t\t\n \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">ER \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">R; SP1/Thermo Scientific \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">1 ∶ 500 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Pressure cooker, 9 min \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">PR \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">M; PgR636/Dako \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">1 ∶ 1000 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Pressure cooker, 9 min \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">HER2 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">R; SP3/Thermo Scientific \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">1 ∶ 100 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Microwave oven \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">p53 protein \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">M; DO-7/Dako \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">1 ∶ 2700 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Pressure cooker, 8 min \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Ki-67 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">M; MIB1/Dako \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">1 ∶ 600 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Pressure cooker, 8 min \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">EGFR \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">M; 31G7/Zymed \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">1 ∶ 200 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">0.1% Pronase, RT, 15 min \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">p63 protein \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">M; 4A4/Dako \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">1 ∶ 300 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Pressure cooker, 8 min \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Vimentin \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">M; V9/Dako \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">1 ∶ 200 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Microwave oven \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">c-kit (CD117) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">R; polyclonal/Dako \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">1 ∶ 50 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Microwave oven \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">CK5/6 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">M; D5/16B4/Dako \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">1 ∶ 100 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Microwave oven \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] ] ] ] "descripcion" => array:1 [ "en" => "Reagents and methods used for immunohistochemical analysis." ] ] 3 => array:7 [ "identificador" => "t2-cln_65p1033" "etiqueta" => "Table 2" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "tabla" => array:2 [ "tablatextoimagen" => array:1 [ 0 => array:1 [ "tabla" => array:1 [ 0 => """ <table border="0" frame="\n \t\t\t\t\tvoid\n \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="left" valign="bottom" scope="col" style="border-bottom: 2px solid black">Variable \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="center" valign="bottom" scope="col" style="border-bottom: 2px solid black">≤ 35 years (n = 213) N (%) \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="center" valign="bottom" scope="col" style="border-bottom: 2px solid black">≥ 60 years (n = 117) N (%) \t\t\t\t\t\t\n \t\t\t\t\t\t</th><th class="td" title="\n \t\t\t\t\ttable-head\n \t\t\t\t " align="center" valign="bottom" scope="col" style="border-bottom: 2px solid black">p-value<a class="elsevierStyleCrossRef" href="#tfn4-cln_65p1033">1</a> \t\t\t\t\t\t\n \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">TN carcinomas \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">74 (34.7) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">19 (16.2) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">0.0004 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Histological grade 3 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">56 (75.7) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">14 (73.7) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="center" valign="top">NS<a class="elsevierStyleCrossRef" href="#tfn5-cln_65p1033">2</a> \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Nuclear grade 3 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">55 (74.3) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">14 (73.7) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="center" valign="top">NS \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">LVI<a class="elsevierStyleCrossRef" href="#tfn6-cln_65p1033">3</a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">12 (16.2) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">2 (10.5) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="center" valign="top">NS \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Tumoral necrosis \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">39 (52.7) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">6 (31.6) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="center" valign="top">NS \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Basal-like molecular profile<a class="elsevierStyleCrossRef" href="#tfn7-cln_65p1033">4</a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">55 (74.3) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">13 (68.4) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="center" valign="top">NS \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">CK5/6 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">7 (9.4) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">8 (42.1) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">0.0005 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">EGFR \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">53 (71.6) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">9 (47.3) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">0.0045 \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Ki-67 (>25%) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">51 (68.9) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">12 (63.1) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="center" valign="top">NS \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">p63 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">1 (1.3) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="center" valign="top">0 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="center" valign="top">NS \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">p53 \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">26 (35.1) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">7 (36.8) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="center" valign="top">NS \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">Vimentin \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">31/71 (43.7)<a class="elsevierStyleCrossRef" href="#tfn8-cln_65p1033">5</a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">5/18 (27.8)<a class="elsevierStyleCrossRef" href="#tfn8-cln_65p1033">5</a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="center" valign="top">NS \t\t\t\t\t\t\n \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="left" valign="top">c-kit \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">15/71 (21.2)<a class="elsevierStyleCrossRef" href="#tfn8-cln_65p1033">5</a> \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="char" valign="top">7 (36.8) \t\t\t\t\t\t\n \t\t\t\t</td><td class="td" title="\n \t\t\t\t\ttable-entry\n \t\t\t\t " align="center" valign="top">NS \t\t\t\t\t\t\n \t\t\t\t</td></tr></tbody></table> """ ] ] ] "notaPie" => array:5 [ 0 => array:3 [ "identificador" => "tfn4-cln_65p1033" "etiqueta" => "1" "nota" => "Pearson's chi-square test" ] 1 => array:3 [ "identificador" => "tfn5-cln_65p1033" "etiqueta" => "2" "nota" => "Non-significant" ] 2 => array:3 [ "identificador" => "tfn6-cln_65p1033" "etiqueta" => "3" "nota" => "Lymphovascular invasion" ] 3 => array:3 [ "identificador" => "tfn7-cln_65p1033" "etiqueta" => "4" "nota" => "EGFR and/or CK5/6 positive" ] 4 => array:3 [ "identificador" => "tfn8-cln_65p1033" "etiqueta" => "5" "nota" => "Two cases in the younger group and one case in the older group could not be assessed" ] ] ] "descripcion" => array:1 [ "en" => "Pathological and immunohistochemical features of TN Breast carcinomas in younger (≤ 35 years) vs. older (≥ 60 years) patients." ] ] ] "bibliografia" => array:2 [ "titulo" => "REFERENCES" "seccion" => array:1 [ 0 => array:2 [ "identificador" => "cebibsec10" "bibliografiaReferencia" => array:27 [ 0 => array:3 [ "identificador" => "bib1" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Effects of young age at presentation on survival in breast cancer" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ NS El Saghir \n \t\t\t\t\t\t\t\t """ 1 => """ M Seoud \n \t\t\t\t\t\t\t\t """ 2 => """ MK Khalil \n \t\t\t\t\t\t\t\t """ 3 => """ M Charafeddine \n \t\t\t\t\t\t\t\t """ 4 => """ ZK Salem \n \t\t\t\t\t\t\t\t """ 5 => """ FB Geara \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:1 [ "Revista" => array:4 [ "tituloSerie" => "BMC Cancer" "fecha" => "2006" "volumen" => "6" "paginaInicial" => "194" ] ] 1 => array:1 [ "WWW" => array:1 [ "link" => "10.1186/1471-2407-6-194" ] ] ] ] ] ] 1 => array:3 [ "identificador" => "bib2" "etiqueta" => "2" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Young age at diagnosis correlates with worse prognosis and defines a subset of breast cancers with shared patterns of gene expression" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ CK Anders \n \t\t\t\t\t\t\t\t """ 1 => """ DS Hsu \n \t\t\t\t\t\t\t\t """ 2 => """ G Broadwater \n \t\t\t\t\t\t\t\t """ 3 => """ CR Acharya \n \t\t\t\t\t\t\t\t """ 4 => """ JA Foekens \n \t\t\t\t\t\t\t\t """ 5 => """ Y Zhang \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1200/JCO.2007.14.2471" "Revista" => array:6 [ "tituloSerie" => "J Clin Oncol" "fecha" => "2008" "volumen" => "26" "paginaInicial" => "3324" "paginaFinal" => "3330" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18612148" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1200/JCO.2007.14.2471" "WWW" => array:1 [ "link" => "10.1200/JCO.2007.14.2471" ] ] ] ] ] ] 2 => array:3 [ "identificador" => "bib3" "etiqueta" => "3" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Breast cancer in young women: poor survival despite intensive treatment" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => """ H Fredholm \n \t\t\t\t\t\t\t\t """ 1 => """ S Eaker \n \t\t\t\t\t\t\t\t """ 2 => """ J Frisell \n \t\t\t\t\t\t\t\t """ 3 => """ L Holmberg \n \t\t\t\t\t\t\t\t """ 4 => """ I Fredriksson \n \t\t\t\t\t\t\t\t """ 5 => """ H Lindman \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:1 [ "Revista" => array:3 [ "tituloSerie" => "PLoS ONE" "fecha" => "2009" "volumen" => "4" ] ] 1 => array:1 [ "WWW" => array:1 [ "link" => "10.1371/journal.pone.0007695" ] ] ] ] ] ] 3 => array:3 [ "identificador" => "bib4" "etiqueta" => "4" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Prevalence, morphologic features and proliferation indices of breast carcinoma molecular classes using immunohistochemical surrogate markers" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ R Bhargava \n \t\t\t\t\t\t\t\t """ 1 => """ J Striebel \n \t\t\t\t\t\t\t\t """ 2 => """ S Beriwal \n \t\t\t\t\t\t\t\t """ 3 => """ JC Flickinger \n \t\t\t\t\t\t\t\t """ 4 => """ A Onisko \n \t\t\t\t\t\t\t\t """ 5 => """ G Ahrendt \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Int J Clin Exp Pathol" "fecha" => "2009" "volumen" => "2" "paginaInicial" => "444" "paginaFinal" => "455" ] ] ] ] ] ] 4 => array:3 [ "identificador" => "bib5" "etiqueta" => "5" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Distinct molecular mechanisms underlying clinically relevant subtypes of breast cancer: gene expression analyses across three different platforms" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ T Sorlie \n \t\t\t\t\t\t\t\t """ 1 => """ Y Wang \n \t\t\t\t\t\t\t\t """ 2 => """ C Xiao \n \t\t\t\t\t\t\t\t """ 3 => """ H Johnsen \n \t\t\t\t\t\t\t\t """ 4 => """ B Naume \n \t\t\t\t\t\t\t\t """ 5 => """ RR Samaha \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1186/1471-2164-7-127" "Revista" => array:5 [ "tituloSerie" => "BMC Genomics" "fecha" => "2001" "volumen" => "7" "paginaInicial" => "127" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16729877" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1186/1471-2164-7-127" "WWW" => array:1 [ "link" => "10.1186/1471-2164-7-127" ] ] ] ] ] ] 5 => array:3 [ "identificador" => "bib6" "etiqueta" => "6" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Molecular portraits of human breast tumours" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ CM Perou \n \t\t\t\t\t\t\t\t """ 1 => """ T Sorlie \n \t\t\t\t\t\t\t\t """ 2 => """ MB Eisen \n \t\t\t\t\t\t\t\t """ 3 => """ M van de Rijn \n \t\t\t\t\t\t\t\t """ 4 => """ SS Jeffrey \n \t\t\t\t\t\t\t\t """ 5 => """ CA Rees \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1038/35021093" "Revista" => array:6 [ "tituloSerie" => "Nature" "fecha" => "2000" "volumen" => "406" "paginaInicial" => "747" "paginaFinal" => "752" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10963602" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1038/35021093" "WWW" => array:1 [ "link" => "10.1038/35021093" ] ] ] ] ] ] 6 => array:3 [ "identificador" => "bib7" "etiqueta" => "7" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Prognostic markers in triple-negative breast cancer" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => """ EA Rakha \n \t\t\t\t\t\t\t\t """ 1 => """ ME El-Sayed \n \t\t\t\t\t\t\t\t """ 2 => """ AR Green \n \t\t\t\t\t\t\t\t """ 3 => """ AH Lee \n \t\t\t\t\t\t\t\t """ 4 => """ JF Robertson \n \t\t\t\t\t\t\t\t """ 5 => """ IO Ellis \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1002/cncr.22381" "Revista" => array:6 [ "tituloSerie" => "Cancer" "fecha" => "2007" "volumen" => "109" "paginaInicial" => "25" "paginaFinal" => "32" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17146782" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1002/cncr.22381" "WWW" => array:1 [ "link" => "10.1002/cncr.22381" ] ] ] ] ] ] 7 => array:3 [ "identificador" => "bib8" "etiqueta" => "8" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Race and triple negative threats to breast cancer survival: a population-based study in Atlanta, GA" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ MJ Lund \n \t\t\t\t\t\t\t\t """ 1 => """ KF Trivers \n \t\t\t\t\t\t\t\t """ 2 => """ PL Porter \n \t\t\t\t\t\t\t\t """ 3 => """ RJ Coates \n \t\t\t\t\t\t\t\t """ 4 => """ B Leyland-Jones \n \t\t\t\t\t\t\t\t """ 5 => """ OW Brawley \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1007/s10549-008-9926-3" "Revista" => array:6 [ "tituloSerie" => "Breast Cancer Res Treat" "fecha" => "2009" "volumen" => "113" "paginaInicial" => "357" "paginaFinal" => "370" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18324472" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1007/s10549-008-9926-3" "WWW" => array:1 [ "link" => "10.1007/s10549-008-9926-3" ] ] ] ] ] ] 8 => array:3 [ "identificador" => "bib9" "etiqueta" => "9" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Variation in breast cancer subtypes with age and race/ethnicity" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:3 [ 0 => """ CA Parise \n \t\t\t\t\t\t\t\t """ 1 => """ KR Bauer \n \t\t\t\t\t\t\t\t """ 2 => """ V Caggiano \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:1 [ 0 => array:1 [ "Revista" => array:2 [ "tituloSerie" => "Crit Rev Oncol Hematol" "fecha" => "2009" ] ] ] ] ] ] 9 => array:3 [ "identificador" => "bib10" "etiqueta" => "10" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Descriptive analysis of estrogen receptor (ER)-negative, progesterone receptor (PR)-negative, and HER2-negative invasive breast cancer, the so-called triple-negative phenotype: a population-based study from the California cancer Registry" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:5 [ 0 => """ KR Bauer \n \t\t\t\t\t\t\t\t """ 1 => """ M Brown \n \t\t\t\t\t\t\t\t """ 2 => """ RD Cress \n \t\t\t\t\t\t\t\t """ 3 => """ CA Parise \n \t\t\t\t\t\t\t\t """ 4 => """ V Caggiano \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:1 [ "Revista" => array:5 [ "tituloSerie" => "Cancer" "fecha" => "2007" "volumen" => "109" "paginaInicial" => "1721" "paginaFinal" => "1728" ] ] 1 => array:1 [ "WWW" => array:1 [ "link" => "10.1002/cncr.22618" ] ] ] ] ] ] 10 => array:3 [ "identificador" => "bib11" "etiqueta" => "11" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Expression of epidermal growth factor receptor in relation to BRCA1 status, basal-like markers and prognosis in breast cancer" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ JB Arnes \n \t\t\t\t\t\t\t\t """ 1 => """ LR Begin \n \t\t\t\t\t\t\t\t """ 2 => """ I Stefansson \n \t\t\t\t\t\t\t\t """ 3 => """ JS Brunet \n \t\t\t\t\t\t\t\t """ 4 => """ TO Nielsen \n \t\t\t\t\t\t\t\t """ 5 => """ WD Foulkes \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1136/jcp.2008.056291" "Revista" => array:6 [ "tituloSerie" => "J Clin Pathol" "fecha" => "2009" "volumen" => "62" "paginaInicial" => "139" "paginaFinal" => "146" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18682421" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1136/jcp.2008.056291" "WWW" => array:1 [ "link" => "10.1136/jcp.2008.056291" ] ] ] ] ] ] 11 => array:3 [ "identificador" => "bib12" "etiqueta" => "12" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Immunohistochemical and clinical characterization of the basal-like subtype of invasive breast carcinoma" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ TO Nielsen \n \t\t\t\t\t\t\t\t """ 1 => """ FD Hsu \n \t\t\t\t\t\t\t\t """ 2 => """ K Jensen \n \t\t\t\t\t\t\t\t """ 3 => """ M Cheang \n \t\t\t\t\t\t\t\t """ 4 => """ G Karaca \n \t\t\t\t\t\t\t\t """ 5 => """ Z Hu \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1158/1078-0432.CCR-04-0220" "Revista" => array:6 [ "tituloSerie" => "Clin Cancer Res" "fecha" => "2004" "volumen" => "10" "paginaInicial" => "5367" "paginaFinal" => "5374" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15328174" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1158/1078-0432.CCR-04-0220" "WWW" => array:1 [ "link" => "10.1158/1078-0432.CCR-04-0220" ] ] ] ] ] ] 12 => array:3 [ "identificador" => "bib13" "etiqueta" => "13" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Is 'basal-like’ carcinoma of the breast a distinct clinicopathological entity? 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invasive breast carcinoma" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ CA Livasy \n \t\t\t\t\t\t\t\t """ 1 => """ G Karaca \n \t\t\t\t\t\t\t\t """ 2 => """ R Nanda \n \t\t\t\t\t\t\t\t """ 3 => """ MS Tretiakova \n \t\t\t\t\t\t\t\t """ 4 => """ OI Olopade \n \t\t\t\t\t\t\t\t """ 5 => """ DT Moore \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1038/modpathol.3800528" "Revista" => array:6 [ "tituloSerie" => "Mod Pathol" "fecha" => "2006" "volumen" => "19" "paginaInicial" => "264" "paginaFinal" => "271" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16341146" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1038/modpathol.3800528" "WWW" => array:1 [ "link" => "10.1038/modpathol.3800528" ] ] ] ] ] ] 21 => array:3 [ "identificador" => "bib22" "etiqueta" => "22" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "The phenotypic 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"autores" => array:6 [ 0 => """ E Korsching \n \t\t\t\t\t\t\t\t """ 1 => """ SS Jeffrey \n \t\t\t\t\t\t\t\t """ 2 => """ W Meinerz \n \t\t\t\t\t\t\t\t """ 3 => """ T Decker \n \t\t\t\t\t\t\t\t """ 4 => """ W Boecker \n \t\t\t\t\t\t\t\t """ 5 => """ H Buerger \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1136/jcp.2008.055475" "Revista" => array:6 [ "tituloSerie" => "J Clin Pathol" "fecha" => "2008" "volumen" => "61" "paginaInicial" => "553" "paginaFinal" => "560" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18326009" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1136/jcp.2008.055475" "WWW" => array:1 [ "link" => "10.1136/jcp.2008.055475" ] ] ] ] ] ] 23 => array:3 [ "identificador" => "bib24" "etiqueta" => "24" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Morphological and immunophenotypic analysis of breast carcinomas with basal and myoepithelial differentiation" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ EA Rakha \n \t\t\t\t\t\t\t\t """ 1 => """ TC Putti \n \t\t\t\t\t\t\t\t """ 2 => """ DM Abd El-Rehim \n \t\t\t\t\t\t\t\t """ 3 => """ C Paish \n \t\t\t\t\t\t\t\t """ 4 => """ AR Green \n \t\t\t\t\t\t\t\t """ 5 => """ DG Powe \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1002/path.1916" "Revista" => array:6 [ "tituloSerie" => "J Pathol" "fecha" => "2006" "volumen" => "208" "paginaInicial" => "495" "paginaFinal" => "506" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16429394" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1002/path.1916" "WWW" => array:1 [ "link" => "10.1002/path.1916" ] ] ] ] ] ] 24 => array:3 [ "identificador" => "bib25" "etiqueta" => "25" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Breast cancer in women under 40years of age: A series of 57 cases from Northern Ireland" "autores" => array:1 [ 0 => array:2 [ "etal" => false "autores" => array:6 [ 0 => """ B McAree \n \t\t\t\t\t\t\t\t """ 1 => """ ME O'Donnell \n \t\t\t\t\t\t\t\t """ 2 => """ A Spence \n \t\t\t\t\t\t\t\t """ 3 => """ TF Lioe \n \t\t\t\t\t\t\t\t """ 4 => """ DT McManus \n \t\t\t\t\t\t\t\t """ 5 => """ RA Spence \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1016/j.breast.2009.12.002" "Revista" => array:6 [ "tituloSerie" => "Breast" "fecha" => "2010" "volumen" => "19" "paginaInicial" => "97" "paginaFinal" => "104" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20060718" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1016/j.breast.2009.12.002" "WWW" => array:1 [ "link" => "10.1016/j.breast.2009.12.002" ] ] ] ] ] ] 25 => array:3 [ "identificador" => "bib26" "etiqueta" => "26" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Basal cytokeratin and epidermal growth factor receptor expression are not predictive of BRCA1 mutation status in women with triple-negative breast cancers" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ LC Collins \n \t\t\t\t\t\t\t\t """ 1 => """ A Martyniak \n \t\t\t\t\t\t\t\t """ 2 => """ MJ Kandel \n \t\t\t\t\t\t\t\t """ 3 => """ ZK Stadler \n \t\t\t\t\t\t\t\t """ 4 => """ S Masciari \n \t\t\t\t\t\t\t\t """ 5 => """ A Miron \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1097/PAS.0b013e31819c1c93" "Revista" => array:6 [ "tituloSerie" => "Am J Surg Pathol" "fecha" => "2009" "volumen" => "33" "paginaInicial" => "1093" "paginaFinal" => "1097" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19390427" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1097/PAS.0b013e31819c1c93" "WWW" => array:1 [ "link" => "10.1097/PAS.0b013e3181b7cb7a" ] ] ] ] ] ] 26 => array:3 [ "identificador" => "bib27" "etiqueta" => "27" "referencia" => array:1 [ 0 => array:2 [ "contribucion" => array:1 [ 0 => array:2 [ "titulo" => "Epidermal growth factor receptor expression in breast cancer association with biologic phenotype and clinical outcomes" "autores" => array:1 [ 0 => array:2 [ "etal" => true "autores" => array:6 [ 0 => """ MF Rimawi \n \t\t\t\t\t\t\t\t """ 1 => """ PB Shetty \n \t\t\t\t\t\t\t\t """ 2 => """ HL Weiss \n \t\t\t\t\t\t\t\t """ 3 => """ R Schiff \n \t\t\t\t\t\t\t\t """ 4 => """ CK Osborne \n \t\t\t\t\t\t\t\t """ 5 => """ GC Chamness \n \t\t\t\t\t\t\t\t """ ] ] ] ] ] "host" => array:2 [ 0 => array:2 [ "doi" => "10.1002/cncr.24816" "Revista" => array:6 [ "tituloSerie" => "Cancer" "fecha" => "2010" "volumen" => "116" "paginaInicial" => "1234" "paginaFinal" => "1242" "link" => array:1 [ 0 => array:2 [ "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20082448" "web" => "Medline" ] ] ] ] 1 => array:2 [ "doi" => "10.1002/cncr.24816" "WWW" => array:1 [ "link" => 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"vista" => "all" ] ] ] "idiomaDefecto" => "en" "url" => "/18075932/0000006500000010/v1_202212010724/S1807593222029313/v1_202212010724/en/main.assets" "Apartado" => null "PDF" => "https://static.elsevier.es/multimedia/18075932/0000006500000010/v1_202212010724/S1807593222029313/v1_202212010724/en/main.pdf?idApp=UINPBA00004N&text.app=https://www.elsevier.es/" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/S1807593222029313?idApp=UINPBA00004N"]

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Filomena M CarvalhoI,

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, Livia M BacchiI, Priscila P C SantosI, Carlos E BacchiII

I Department of Pathology, Faculdade de Medicina, Universidade de São Paulo, São Paulo, SP, Brazil

II Consultoria em Patologia, Botucatu, SP, Brazil

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            "en" => "<p id="spara40" class="elsevierStyleSimplePara elsevierViewall">Effects of diazepam and <span class="elsevierStyleItalic">D&#46; anethifolia</span> extract on &#40;A&#41; the latency to loss of righting reflex and &#40;B&#41; total sleep time&#46; The interval between the administration of ketamin until the loss of the righting reflex was recorded as onset of sleep&#46; The time from loss of righting reflex to regaining of the righting reflex was recorded as duration of sleep&#46; Results represent mean &#40;&#177; S&#46;E&#46;M&#46;&#41; from six mice&#46; &#8727;P &#60; 0&#46;01 compared with vehicle-treated control&#46;</p>"
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          "en" => "<p id="spara10" class="elsevierStyleSimplePara elsevierViewall">A high-grade invasive ductal carcinoma &#40;A&#41; with intense&#44; diffuse CK5&#47;6 immunoexpression &#40;B&#41;&#46;</p>"
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    "textoCompleto" => "<span class="elsevierStyleSections"><span id="cesec10" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle50">INTRODUCTION</span><p id="para10" class="elsevierStylePara elsevierViewall">Breast cancer detected in younger patients has been associated with a more aggressive phenotype&#46;<a class="elsevierStyleCrossRefs" href="#bib1">1-3</a> To improve treatment outcomes and to reduce mortality from this disease&#44; a greater understanding of this aggressive phenotype is needed&#46; Gene-expression profiling using DNA microarrays has identified five subtypes of breast cancer &#40;i&#46;e&#46;&#44; luminal A&#44; luminal B&#44; normal breast-like&#44; HER2-overexpression&#44; and basal-like&#41;&#46; Each of these subtypes is associated with a distinct prognosis&#44; but the different subtypes share similarities&#46; For example&#44; immunohistochemistry has been used to evaluate the expression of estrogen receptors &#40;ERs&#41;&#44; progesterone receptors &#40;PRs&#41;&#44; HER2&#44; and Ki-67 to characterize the different subtypes&#46;<a class="elsevierStyleCrossRef" href="#bib4">4</a> The basal-like and HER2&#43; subtypes both have a shorter relapse-free survival and overall survival than luminal tumors&#46;<a class="elsevierStyleCrossRefs" href="#bib5">5&#44;6</a> Triple-negative &#40;TN&#41; breast carcinomas &#40;i&#46;e&#46;&#44; ER-negative&#44; PR-negative&#44; and HER2-negative tumors&#41; have been shown to be related to a basal-like phenotype and&#44; accordingly&#44; exhibit more aggressive clinical and pathologic features&#46;<a class="elsevierStyleCrossRef" href="#bib7">7</a> Triple-negative carcinomas are also more prevalent among specific subgroups of women&#44; particularly younger patients&#44; and have been found to be associated with <span class="elsevierStyleItalic">BRCA1</span> germline mutations&#46;<a class="elsevierStyleCrossRefs" href="#bib8">8-11</a> Although significant overlap in criteria of classification of TN&#44; basal-like carcinomas&#44; and <span class="elsevierStyleItalic">BRCA1</span>-related tumors has been shown&#44; these three tumor types are not synonymous&#46; Therefore&#44; no consensus exists regarding the criteria that identify basal-like subgroups of TN carcinomas&#46; Currently&#44; the most useful criteria have been the expression of basal-cytokeratin &#40;CK5&#47;6&#41; and&#47;or epidermal growth factor receptor &#40;EGFR&#41;&#59;<a class="elsevierStyleCrossRef" href="#bib12">12</a> however&#44; recent studies have indicated that TN carcinomas are not as homogeneous as they were first thought to be&#46;<a class="elsevierStyleCrossRefs" href="#bib7">7&#44;13&#44;14</a> In this study&#44; the frequencies and phenotypic characteristics of TN and basal-like carcinomas in patients who were 35 years old or younger were analyzed and compared with those of TN carcinomas diagnosed in patients who were 60 years old and older&#46;</p></span><span id="cesec20" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle60">METHODS AND MATERIALS</span><p id="para20" class="elsevierStylePara elsevierViewall">This project was approved by the Scientific Committee of the Department of Pathology of the Faculdade de Medicina da Universidade de Sao Paulo and by the Ethical Committee for Research Projects of the Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo &#40;CAPPesq&#41; &#40;protocol 563&#47;07&#41;&#46; Between 1997 and 2007&#44; 213 tumor specimens from patients who were 35 years old or younger were registered at the Consultoria em Patologia&#44; a large reference laboratory located in Botucatu&#44; Sao Paulo&#44; Brazil&#46; An additional 117 tumor samples from patients who were 60 years old or older were also analyzed by the same laboratory in 2006&#46; All tumor samples from the two age groups were reviewed and classified according to histological type&#44; histological grade&#44; nuclear grade&#44; the presence of tumor necrosis&#44; the presence of an <span class="elsevierStyleItalic">in situ</span> component&#44; and any vascular involvement&#46;</p><p id="para30" class="elsevierStylePara elsevierViewall">For each tumor&#44; representative areas were selected to construct tissue microarrays &#40;TMAs&#41;&#46; Briefly&#44; three cylinders&#44; each 1&#46;5 mm in diameter&#44; were removed from selected areas of donor blocks and mounted into paraffin blocks at 1-mm intervals using a precision microarray instrument &#40;Beecher Instruments&#44; Silver Spring&#44; MD&#41;&#46; A grid system was established&#44; and each core had a coordinate reference &#40;i&#46;e&#46;&#44; x-axis&#44; y-axis&#41; for sample identification&#46; Blocks were sealed at 60&#176;C for 10 min&#44; and 5-&#181;m sections of the resulting TMA blocks were prepared using standard techniques and mounted on Starfrost&#174; slides&#46;</p><p id="para40" class="elsevierStylePara elsevierViewall">Histological sections from the TMA blocks were immunostained for ER&#44; PR&#44; HER2&#44; Ki-67 antigen&#44; EGFR&#44; CK5&#47;6&#44; p53&#44; vimentin&#44; CD117&#44; and p63&#46; The sources and dilutions of the antibodies and the epitope retrieval methods that were used are listed in <a class="elsevierStyleCrossRef" href="#t1-cln_65p1033">Table 1</a>&#46; Bound antibodies were detected using Novolink system &#40;Leica&#44; USA&#41;&#46;</p><elsevierMultimedia ident="t1-cln_65p1033"></elsevierMultimedia><p id="para50" class="elsevierStylePara elsevierViewall">Positive immunohistochemistry staining was used to identify cytoplasmic localization of CK5&#47;6&#44; vimentin&#44; and CD117&#59; nuclear localization of ER&#44; PR&#44; Ki-67&#44; p63&#44; and p53&#59; and membranous-pattern staining of EGFR and HER2&#46; In this study&#44; only cases that had no ER- or PR-positive cells&#44; were negative for HER2&#44; and were scored as 0 or 1&#43; according to the guidelines of the American Society of Clinical Oncology &#40;ASCO&#41; and College of American Pathologists &#40;CAP&#41;<a class="elsevierStyleCrossRef" href="#bib15">15</a> were included&#46; Ki-67 expression was scored as either &#60; 25&#37; or &#62; 25&#37;&#46; For the other markers&#44; at least 1&#37; of the positive cells with a moderate to strong intensity were considered positive&#46;</p><p id="para60" class="elsevierStylePara elsevierViewall">Statistical analysis was performed using a two-way contingency table and the chi-square test&#46;</p></span><span id="cesec30" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle70">RESULTS</span><p id="para70" class="elsevierStylePara elsevierViewall">Triple-negative breast carcinomas were diagnosed in 74&#47;213 &#40;34&#46;7&#37;&#41; patients who were 35 years old or younger and in 19&#47;117 &#40;16&#46;2&#37;&#41; patients who were 60 years old or older &#40;p &#61; 0&#46;002&#41;&#46; The median ages of the younger and older patients who were included in the study were 32 years &#40;mean &#177; SD&#58; 30&#46;9 &#177; 3&#46;94&#59; range&#58; 21-35 years&#41; and 73 years &#40;mean &#177; SD&#58; 73&#46;7 &#177; 7&#46;53&#59; range&#58; 61-91 years&#41;&#44; respectively&#46; In the younger group&#44; 67&#47;74 &#40;90&#46;5&#37;&#41; tumors were of a ductal histological type&#59; other histological types identified included metaplastic &#40;2 cases&#41;&#44; pleomorphic lobular &#40;2 cases&#41;&#44; secretory &#40;1 case&#41;&#44; mucinous &#40;1 case&#41;&#44; and medullary &#40;1 case&#41;&#46; Regarding the older patients&#44; 15&#47;19 &#40;78&#46;9&#37;&#41; tumors were of a ductal histological type&#44; and 4 &#40;21&#46;1&#37;&#41; were of a pleomorphic lobular type&#46; The pathological features of the tumors that were identified in each patient group are summarized in <a class="elsevierStyleCrossRef" href="#t2-cln_65p1033">Table 2</a>&#46; The histological and nuclear grades and the angiovascular involvement were not different between the two patient groups&#46; Tumoral necrosis was more frequently observed in tumors that were derived from younger patients than in those from older patients &#40;52&#46;7&#37; vs&#46; 31&#46;6&#37;&#44; respectively&#41;&#46; However&#44; the difference was not statistically significant&#46; The basal phenotype&#44; which was determined by the expression of EGFR and&#47;or CK5&#47;6 according to the criteria of Nielsen et al&#46;&#44;<a class="elsevierStyleCrossRef" href="#bib12">12</a> was similar for both groups &#40;74&#46;3&#37; for younger patients <span class="elsevierStyleItalic">vs&#46;</span> 68&#46;4&#37; for older patients&#41;&#46; However&#44; the two markers showed differences in the frequency between the two groups &#40;<a class="elsevierStyleCrossRefs" href="#fig1-cln_65p1033">Figures 1 and 2</a>&#41;&#46; Tumors that were derived from the younger patients were more frequently EGFR positive &#40;71&#46;6&#37; <span class="elsevierStyleItalic">vs&#46;</span> 47&#46;3&#37;&#44; respectively&#41; and exhibited a lower frequency of CK5&#47;6 expression &#40;9&#46;4&#37; <span class="elsevierStyleItalic">vs&#46;</span> 42&#46;1&#37;&#44; respectively&#41;&#46; Regarding the basal-like tumors of younger patients&#44; 53&#47;55 &#40;96&#46;4&#37;&#41; were associated with EGFR expression&#44; whereas in the older patient group&#44; 9&#47;13 &#40;69&#46;2&#37;&#41; expressed EGFR &#40;p &#61; 0&#46;0018&#41;&#46; c-KIT expression was also observed more frequently in tumors that occurred in older patients &#40;36&#46;8&#37;&#41; than in younger patients &#40;21&#46;2&#37;&#41;&#44; although the difference was not significant&#46; The analysis of other markers&#44; including Ki-67&#44; p63&#44; p53&#44; and vimentin&#44; did not show any significant differences between the two groups&#46;</p><elsevierMultimedia ident="t2-cln_65p1033"></elsevierMultimedia><elsevierMultimedia ident="fig1-cln_65p1033"></elsevierMultimedia><elsevierMultimedia ident="fig2-cln_65p1033"></elsevierMultimedia></span><span id="cesec40" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle80">DISCUSSION</span><p id="para80" class="elsevierStylePara elsevierViewall">Breast cancer that is detected in younger women is typically associated with aggressive behavior and results in a poor prognosis&#46; Unfortunately&#44; the specific mechanisms responsible for this tumor phenotype remain unclear&#46; This poor understanding is further complicated by the controversial results of many studies&#46; Currently&#44; breast cancers that are diagnosed in younger patients are characterized by reduced hormone sensitivity and high HER2&#47;EGFR expression&#44;<a class="elsevierStyleCrossRefs" href="#bib2">2&#44;16</a> although ER-positive carcinomas are still a prevalent subgroup of tumors in this age group&#46;<a class="elsevierStyleCrossRefs" href="#bib1">1&#44;16</a> Furthermore&#44; luminal carcinomas in younger women are mostly subtype B&#44; which is determined by their frequent co-expression of HER2 and&#47;or their high proliferative activity&#46;<a class="elsevierStyleCrossRef" href="#bib16">16</a> Triple-negative carcinomas tend to occur less frequently than luminal carcinomas&#59; however&#44; triple-negative carcinomas are more prevalent in patients who are younger than 35 years old&#46;<a class="elsevierStyleCrossRef" href="#bib17">17</a></p><p id="para90" class="elsevierStylePara elsevierViewall">TN carcinomas are considered to be a group of biologically distinct neoplasias that mostly exhibit a basal phenotype and an aggressive biology&#46; Unlike the other subtypes&#44; targeted agents that are specifically aimed at triple-negative breast tumors are not yet available&#59; thus&#44; this deficiency intensifies the need and interest in advancing novel therapeutic strategies beyond chemotherapy for this subset of high-risk patients&#46; Triple-negative is a term based on clinical assays for ER&#44; PR&#44; and HER2&#44; whereas basal-like is a molecular phenotype that was initially defined using cDNA microarrays&#46; However&#44; low-grade tumors&#44; such as adenoid cystic and medullary carcinomas&#44; are also considered TN carcinomas&#46;<a class="elsevierStyleCrossRefs" href="#bib13">13&#44;18</a> Therefore&#44; the best criteria to characterize the phenotype of basal carcinomas have remained somewhat controversial&#46; Nielsen et al&#46; proposed that the best immunohistochemistry predictors of basal-like gene expression are the positive expression of CK 5&#47;6 and EGFR and the negative expression of HER2 and ER&#59; these criteria were associated with a sensitivity of 76&#37; and a specificity 100&#37;&#46;<a class="elsevierStyleCrossRef" href="#bib12">12</a> In this study&#44; we analyzed these markers&#59; however&#44; it should be noted that other studies have considered the expression patterns of CK5&#44; CK14&#44; CK 17&#44; p-cadherin&#44; p63&#44; c-kit&#44; and vimentin to characterize a basal-like phenotype&#46;<a class="elsevierStyleCrossRefs" href="#bib19">19-22</a> Differences in the molecular profile of basal-like carcinomas have also been associated with protein expression patterns of physiological stem&#47;progenitor cells during breast development&#46;<a class="elsevierStyleCrossRef" href="#bib23">23</a> In this histogenetic model&#44; adult CK5-positive stem cells are hypothesized to differentiate into neoplastic stem cells as a result of EGFR amplification&#44; a mutation of p53&#44; or BRCA inactivation&#44; and this differentiation leads to the generation of basal-like carcinomas&#46; In general&#44; these types of tumors express high levels of p-cadherin&#44; EGFR&#44; and CK5 and an absence or low levels of luminal markers&#44; such as CK 8&#47;18&#46;<a class="elsevierStyleCrossRef" href="#bib23">23</a> Given the number of markers involved&#44; the possibility that different expression profiles could define distinct subsets of basal-like carcinomas should be considered&#46; For example&#44; Rakha et al&#46; compared tumors with a basal phenotype that were characterized by the expression of basal cytokeratins &#40;CK5&#47;6 or CK14&#41; with cells with a myoepithelial phenotype &#40;p63 or smooth muscle actin&#41;&#46;<a class="elsevierStyleCrossRef" href="#bib24">24</a> The basal phenotype was associated with a worse outcome&#44; which suggested that although basal and myoepithelial phenotypes share many features&#44; including a similar genetic profile&#44; they possible are distinct tumors&#46;<a class="elsevierStyleCrossRef" href="#bib24">24</a></p><p id="para100" class="elsevierStylePara elsevierViewall">Very few studies exist that have examined molecular differences in breast tumors according to age&#46;<a class="elsevierStyleCrossRefs" href="#bib1">1-3&#44;9&#44;16&#44;25</a> In the present study&#44; a higher prevalence of TN carcinoma was observed in younger patients &#40;34&#46;3&#37; for younger patients <span class="elsevierStyleItalic">vs&#46;</span> 16&#46;3&#37; for older patients&#41;&#44; and a basal-like phenotype associated with positive expression of CK5&#47;6 and&#47;or EGFR was present in 74&#46;3&#37; vs&#46; 68&#46;4&#37;&#44; respectively&#46; More importantly&#44; the molecular profiles of the TN carcinomas from younger patients were different from the profiles of the tumors from older patients&#46; Triple negative carcinomas in the younger age group were more frequently EGFR positive &#40;71&#46;6&#37; <span class="elsevierStyleItalic">vs&#46;</span> 47&#46;3&#37;&#44; respectively&#41; and less frequently CK5&#47;6 positive &#40;9&#46;4&#37; <span class="elsevierStyleItalic">vs&#46;</span> 42&#46;1&#37;&#44; respectively&#41;&#46; The role of EGFR expression in breast cancer has been investigated by many authors&#46;<a class="elsevierStyleCrossRefs" href="#bib11">11&#44;14&#44;26&#44;27</a> Viale et al&#46; reported a worse prognosis for patients with TN carcinomas that had EGFR-immunoreactivity present in more than 50&#37; of neoplastic cells&#44; which suggested a possible prognostic role for quantification&#46;<a class="elsevierStyleCrossRef" href="#bib14">14</a> Similarly&#44; Rimawi et al&#46; used radioligand-binding assays to quantify EGFR expression in frozen sections of breast tissue carcinomas and found that higher levels of EGFR were associated with younger and black women&#44; a more aggressive outcome&#44; and lower levels of hormone receptors&#46;<a class="elsevierStyleCrossRef" href="#bib27">27</a> The relationship between EGFR and BRCA1 has been investigated by different groups&#44; and <span class="elsevierStyleItalic">BRCA1</span>-germline mutations have been associated with a basal-like phenotype and younger age&#46;<a class="elsevierStyleCrossRefs" href="#bib8">8-11</a> In a study by Collins et al&#46; that analyzed BRCA1 in 144 TN carcinomas&#44; independent of the patient&#39;s age&#44;<a class="elsevierStyleCrossRef" href="#bib26">26</a> they found a higher prevalence of basal CK and EGFR among TN breast cancers&#59; however&#44; the frequency of expression of these markers was similar in women with and without <span class="elsevierStyleItalic">BRCA1</span> mutations&#46;<a class="elsevierStyleCrossRef" href="#bib26">26</a> In contrast&#44; Arnes et al&#46; found that EGFR was a predictor of <span class="elsevierStyleItalic">BRCA1</span> status&#44; followed by patient age and ER status&#46;<a class="elsevierStyleCrossRef" href="#bib11">11</a> In the same study&#44; a worse prognosis was associated with a mutation of <span class="elsevierStyleItalic">BRCA1</span> and positive expression of EGFR&#46;<a class="elsevierStyleCrossRef" href="#bib11">11</a></p><p id="para110" class="elsevierStylePara elsevierViewall">As a receptor tyrosine kinase that plays essential roles in both normal physiological conditions and cancerous conditions&#44; EGFR can affect many important characteristics of a cancer&#39;s phenotype&#44; including evasion of apoptosis&#44; proliferation&#44; invasion&#44; and metastasis&#46; In a previous study&#44; the frequency of EGFR-positive tumors among young patients was found to be higher than among older women &#40;5&#46;9&#37; vs&#46; 3&#46;3&#37;&#44; respectively&#41;&#44; although the difference was not statistically significant&#46;<a class="elsevierStyleCrossRef" href="#bib16">16</a> Based on the results of that study and the data presented in this report&#44; EGFR expression appears to play an important role in the early onset of breast cancer&#44; and we hypothesize that this role is related to intrinsic genetic differences that result in an adverse outcome that is associated with the aggressive breast carcinomas&#46; Furthermore&#44; we propose that EGFR immunostaining and&#47;or amplification should be further investigated as predictor of a patient&#39;s response to targeted therapy&#46;</p></span></span>"
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        0 => array:2 [
          "identificador" => "xpalclavsec1581289"
          "titulo" => "KEYWORDS"
        ]
        1 => array:2 [
          "identificador" => "cesec10"
          "titulo" => "INTRODUCTION"
        ]
        2 => array:2 [
          "identificador" => "cesec20"
          "titulo" => "METHODS AND MATERIALS"
        ]
        3 => array:2 [
          "identificador" => "cesec30"
          "titulo" => "RESULTS"
        ]
        4 => array:2 [
          "identificador" => "cesec40"
          "titulo" => "DISCUSSION"
        ]
        5 => array:2 [
          "identificador" => "xack638827"
          "titulo" => "ACKNOWLEDGEMENTS"
        ]
        6 => array:1 [
          "titulo" => "REFERENCES"
        ]
      ]
    ]
    "pdfFichero" => "main.pdf"
    "tienePdf" => true
    "fechaRecibido" => "2010-09-15"
    "fechaAceptado" => "2010-09-20"
    "PalabrasClave" => array:1 [
      "en" => array:1 [
        0 => array:4 [
          "clase" => "keyword"
          "titulo" => "KEYWORDS"
          "identificador" => "xpalclavsec1581289"
          "palabras" => array:5 [
            0 => "Breast cancer"
            1 => "Triple-negative carcinomas"
            2 => "Basal-like Carcinomas"
            3 => "Young women"
            4 => "Immunohistochemistry"
          ]
        ]
      ]
    ]
    "tieneResumen" => true
    "resumen" => array:1 [
      "en" => array:2 [
        "resumen" => "<span id="ceabs10" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle10">OBJECTIVE&#58;</span><p id="spara80" class="elsevierStyleSimplePara elsevierViewall">To compare the frequency and immunohistochemical profiles of triple-negative breast carcinomas in younger and older women&#46;</p></span> <span id="ceabs20" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle20">METHODS AND RESULTS&#58;</span><p id="spara90" class="elsevierStyleSimplePara elsevierViewall">We selected patients diagnosed with triple-negative breast carcinomas&#46; The groups examined were women who were 35 years old or younger between 1997 and 2007 &#40;n &#61; 74&#41; and&#44; for comparison&#44; women who were 60 years old or older &#40;n &#61; 19&#44; consecutive cases&#41;&#46; All formalin-fixed and paraffin-embedded tumor samples were reviewed and immunohistochemically stained for ER&#44; PR&#44; HER2&#44; Ki-67 antigen&#44; epidermal growth factor receptor&#44; cytokeratin 5&#47;6&#44; p53&#44; vimentin&#44; CD117&#44; and p63 using tissue microarrays blocks&#46; Triple-negative breast carcinomas corresponded to 34&#46;6&#37; &#40;74&#47;213&#41; of the carcinomas from the younger patients and 16&#46;2&#37; &#40;19&#47;117&#41; of the carcinomas from the older patients &#40;p &#61; 0&#46;002&#41;&#46; No significant differences in the frequency of the basal phenotype were observed in the two patient groups based on CK5&#47;6 and&#47;or epidermal growth factor receptor expression &#40;74&#46;3&#37; <span class="elsevierStyleItalic">vs&#46;</span> 68&#46;4&#37;&#41;&#46; However&#44; triple-negative breast carcinomas in the older patients presented a higher frequency of CK5&#47;6 expression compared to those of younger patients &#40;42&#46;1&#37; <span class="elsevierStyleItalic">vs&#46;</span> 9&#46;6&#37;&#59; p &#61; 0&#46;005&#41;&#44; whereas triple-negative breast carcinomas of younger patients had a higher expression level of epidermal growth factor receptor &#40;71&#46;6&#37; <span class="elsevierStyleItalic">vs&#46;</span> 47&#46;3&#37;&#41;&#46;</p></span> <span id="ceabs30" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="cestitle30">CONCLUSIONS&#58;</span><p id="spara100" class="elsevierStyleSimplePara elsevierViewall">These results show that there were significant molecular differences between the triple-negative basal-like breast carcinomas that were diagnosed in younger women and those that were diagnosed in older women&#46; These findings may provide a basis for describing the more aggressive phenotype of the triple-negative breast carcinomas observed in younger women&#46;</p></span>"
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            "titulo" => "METHODS AND RESULTS&#58;"
          ]
          2 => array:2 [
            "identificador" => "ceabs30"
            "titulo" => "CONCLUSIONS&#58;"
          ]
        ]
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          "en" => "<p id="spara10" class="elsevierStyleSimplePara elsevierViewall">A high-grade invasive ductal carcinoma &#40;A&#41; with intense&#44; diffuse CK5&#47;6 immunoexpression &#40;B&#41;&#46;</p>"
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          "en" => "<p id="spara20" class="elsevierStyleSimplePara elsevierViewall">A high-grade invasive ductal carcinoma with numerous mitotic figures &#40;A&#41; and EGFR immunoexpression &#40;B&#41;&#46;</p>"
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          "leyenda" => "<p id="spara40" class="elsevierStyleSimplePara elsevierViewall">ER&#58; Estrogen receptor&#59; PR&#58; Progesterone receptor&#59; EGFR&#58; Epidermal growth factor receptor&#59; CK&#58; cytokeratin</p><p id="spara50" class="elsevierStyleSimplePara elsevierViewall">Pressure cooker&#58; citrate buffer &#40;pH 6&#41; &#40;<span class="elsevierStyleItalic">Tender Cooker&#44; Nordic Wave</span>&#44; USA&#41;</p><p id="spara60" class="elsevierStyleSimplePara elsevierViewall">Microwave&#58; citrate buffer &#40;pH 6&#41;&#44; 15 min &#40;Eletrolux&#44; 900 W&#41;</p>"
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                  \t\t\t\t  " align="left" valign="bottom" scope="col" style="border-bottom: 2px solid black">Antigen&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t  " align="center" valign="bottom" scope="col" style="border-bottom: 2px solid black">Clone&#47;Source&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t  " align="center" valign="bottom" scope="col" style="border-bottom: 2px solid black">Dilution&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="center" valign="bottom" scope="col" style="border-bottom: 2px solid black">Epitope retrieval method&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">ER&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">R&#59; SP1&#47;Thermo Scientific&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">1 &#8758; 500&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Pressure cooker&#44; 9 min&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">PR&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">M&#59; PgR636&#47;Dako&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">1 &#8758; 1000&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Pressure cooker&#44; 9 min&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">HER2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">R&#59; SP3&#47;Thermo Scientific&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">1 &#8758; 100&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Microwave oven&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">p53 protein&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">M&#59; DO-7&#47;Dako&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
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                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Pressure cooker&#44; 8 min&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Ki-67&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">M&#59; MIB1&#47;Dako&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">1 &#8758; 600&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Pressure cooker&#44; 8 min&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">EGFR&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">M&#59; 31G7&#47;Zymed&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">1 &#8758; 200&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">0&#46;1&#37; Pronase&#44; RT&#44; 15 min&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">p63 protein&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">M&#59; 4A4&#47;Dako&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">1 &#8758; 300&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Pressure cooker&#44; 8 min&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Vimentin&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">M&#59; V9&#47;Dako&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">1 &#8758; 200&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Microwave oven&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">c-kit &#40;CD117&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">R&#59; polyclonal&#47;Dako&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">1 &#8758; 50&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Microwave oven&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">CK5&#47;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">M&#59; D5&#47;16B4&#47;Dako&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">1 &#8758; 100&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Microwave oven&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
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          "en" => "<p id="spara30" class="elsevierStyleSimplePara elsevierViewall">Reagents and methods used for immunohistochemical analysis&#46;</p>"
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                  <table border="0" frame="\n
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                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="\n
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                  \t\t\t\t  " align="left" valign="bottom" scope="col" style="border-bottom: 2px solid black">Variable&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t  " align="center" valign="bottom" scope="col" style="border-bottom: 2px solid black">&#8804; 35 years &#40;n &#61; 213&#41; N &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t  " align="center" valign="bottom" scope="col" style="border-bottom: 2px solid black">&#8805; 60 years &#40;n &#61; 117&#41; N &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th><th class="td" title="\n
                  \t\t\t\t\ttable-head\n
                  \t\t\t\t  " align="center" valign="bottom" scope="col" style="border-bottom: 2px solid black"><span class="elsevierStyleItalic">p</span>-value<a class="elsevierStyleCrossRef" href="#tfn4-cln_65p1033">1</a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">TN carcinomas&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">74 &#40;34&#46;7&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">19 &#40;16&#46;2&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">0&#46;0004&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Histological grade 3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">56 &#40;75&#46;7&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">14 &#40;73&#46;7&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="top">NS<a class="elsevierStyleCrossRef" href="#tfn5-cln_65p1033">2</a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Nuclear grade 3&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">55 &#40;74&#46;3&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">14 &#40;73&#46;7&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="top">NS&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">LVI<a class="elsevierStyleCrossRef" href="#tfn6-cln_65p1033">3</a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">12 &#40;16&#46;2&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">2 &#40;10&#46;5&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="top">NS&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Tumoral necrosis&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">39 &#40;52&#46;7&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">6 &#40;31&#46;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="top">NS&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Basal-like molecular profile<a class="elsevierStyleCrossRef" href="#tfn7-cln_65p1033">4</a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">55 &#40;74&#46;3&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">13 &#40;68&#46;4&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="top">NS&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">CK5&#47;6&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">7 &#40;9&#46;4&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">8 &#40;42&#46;1&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">0&#46;0005&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">EGFR&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">53 &#40;71&#46;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">9 &#40;47&#46;3&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">0&#46;0045&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Ki-67 &#40;&#62;25&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">51 &#40;68&#46;9&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">12 &#40;63&#46;1&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="top">NS&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">p63&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">1 &#40;1&#46;3&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="top">0&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="top">NS&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">p53&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">26 &#40;35&#46;1&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">7 &#40;36&#46;8&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="top">NS&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">Vimentin&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">31&#47;71 &#40;43&#46;7&#41;<a class="elsevierStyleCrossRef" href="#tfn8-cln_65p1033">5</a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">5&#47;18 &#40;27&#46;8&#41;<a class="elsevierStyleCrossRef" href="#tfn8-cln_65p1033">5</a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="top">NS&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="left" valign="top">c-kit&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">15&#47;71 &#40;21&#46;2&#41;<a class="elsevierStyleCrossRef" href="#tfn8-cln_65p1033">5</a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="char" valign="top">7 &#40;36&#46;8&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="\n
                  \t\t\t\t\ttable-entry\n
                  \t\t\t\t  " align="center" valign="top">NS&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
              ]
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          ]
          "notaPie" => array:5 [
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              "identificador" => "tfn4-cln_65p1033"
              "etiqueta" => "1"
              "nota" => "<p class="elsevierStyleNotepara" id="cenpara10">Pearson&#39;s chi-square test</p>"
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            1 => array:3 [
              "identificador" => "tfn5-cln_65p1033"
              "etiqueta" => "2"
              "nota" => "<p class="elsevierStyleNotepara" id="cenpara20">Non-significant</p>"
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            2 => array:3 [
              "identificador" => "tfn6-cln_65p1033"
              "etiqueta" => "3"
              "nota" => "<p class="elsevierStyleNotepara" id="cenpara30">Lymphovascular invasion</p>"
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            3 => array:3 [
              "identificador" => "tfn7-cln_65p1033"
              "etiqueta" => "4"
              "nota" => "<p class="elsevierStyleNotepara" id="cenpara40">EGFR and&#47;or CK5&#47;6 positive</p>"
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              "etiqueta" => "5"
              "nota" => "<p class="elsevierStyleNotepara" id="cenpara50">Two cases in the younger group and one case in the older group could not be assessed</p>"
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        "descripcion" => array:1 [
          "en" => "<p id="spara70" class="elsevierStyleSimplePara elsevierViewall">Pathological and immunohistochemical features of TN Breast carcinomas in younger &#40;&#8804; 35 years&#41; vs&#46; older &#40;&#8805; 60 years&#41; patients&#46;</p>"
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    ]
    "bibliografia" => array:2 [
      "titulo" => "REFERENCES"
      "seccion" => array:1 [
        0 => array:2 [
          "identificador" => "cebibsec10"
          "bibliografiaReferencia" => array:27 [
            0 => array:3 [
              "identificador" => "bib1"
              "etiqueta" => "1"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Effects of young age at presentation on survival in breast cancer"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              NS El Saghir \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              M Seoud \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              MK Khalil \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              M Charafeddine \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              ZK Salem \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              FB Geara \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:1 [
                      "Revista" => array:4 [
                        "tituloSerie" => "BMC Cancer"
                        "fecha" => "2006"
                        "volumen" => "6"
                        "paginaInicial" => "194"
                      ]
                    ]
                    1 => array:1 [
                      "WWW" => array:1 [
                        "link" => "10&#46;1186&#47;1471-2407-6-194"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            1 => array:3 [
              "identificador" => "bib2"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Young age at diagnosis correlates with worse prognosis and defines a subset of breast cancers with shared patterns of gene expression"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              CK Anders \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              DS Hsu \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              G Broadwater \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              CR Acharya \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              JA Foekens \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              Y Zhang \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1200/JCO.2007.14.2471"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Oncol"
                        "fecha" => "2008"
                        "volumen" => "26"
                        "paginaInicial" => "3324"
                        "paginaFinal" => "3330"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18612148"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1200/JCO.2007.14.2471"
                      "WWW" => array:1 [
                        "link" => "10&#46;1200&#47;JCO&#46;2007&#46;14&#46;2471"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib3"
              "etiqueta" => "3"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Breast cancer in young women&#58; poor survival despite intensive treatment"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => """
                              H Fredholm \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              S Eaker \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              J Frisell \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              L Holmberg \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              I Fredriksson \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              H Lindman \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:1 [
                      "Revista" => array:3 [
                        "tituloSerie" => "PLoS ONE"
                        "fecha" => "2009"
                        "volumen" => "4"
                      ]
                    ]
                    1 => array:1 [
                      "WWW" => array:1 [
                        "link" => "10&#46;1371&#47;journal&#46;pone&#46;0007695"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            3 => array:3 [
              "identificador" => "bib4"
              "etiqueta" => "4"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Prevalence&#44; morphologic features and proliferation indices of breast carcinoma molecular classes using immunohistochemical surrogate markers"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              R Bhargava \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              J Striebel \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              S Beriwal \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              JC Flickinger \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              A Onisko \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              G Ahrendt \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Int J Clin Exp Pathol"
                        "fecha" => "2009"
                        "volumen" => "2"
                        "paginaInicial" => "444"
                        "paginaFinal" => "455"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            4 => array:3 [
              "identificador" => "bib5"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Distinct molecular mechanisms underlying clinically relevant subtypes of breast cancer&#58; gene expression analyses across three different platforms"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              T Sorlie \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              Y Wang \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              C Xiao \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              H Johnsen \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              B Naume \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              RR Samaha \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1186/1471-2164-7-127"
                      "Revista" => array:5 [
                        "tituloSerie" => "BMC Genomics"
                        "fecha" => "2001"
                        "volumen" => "7"
                        "paginaInicial" => "127"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16729877"
                            "web" => "Medline"
                          ]
                        ]
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                    1 => array:2 [
                      "doi" => "10.1186/1471-2164-7-127"
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                        "link" => "10&#46;1186&#47;1471-2164-7-127"
                      ]
                    ]
                  ]
                ]
              ]
            ]
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              "identificador" => "bib6"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Molecular portraits of human breast tumours"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              CM Perou \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              T Sorlie \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              MB Eisen \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              M van de Rijn \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              SS Jeffrey \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              CA Rees \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1038/35021093"
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                        "tituloSerie" => "Nature"
                        "fecha" => "2000"
                        "volumen" => "406"
                        "paginaInicial" => "747"
                        "paginaFinal" => "752"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10963602"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1038/35021093"
                      "WWW" => array:1 [
                        "link" => "10&#46;1038&#47;35021093"
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                ]
              ]
            ]
            6 => array:3 [
              "identificador" => "bib7"
              "etiqueta" => "7"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Prognostic markers in triple-negative breast cancer"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => """
                              EA Rakha \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              ME El-Sayed \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              AR Green \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              AH Lee \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              JF Robertson \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              IO Ellis \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1002/cncr.22381"
                      "Revista" => array:6 [
                        "tituloSerie" => "Cancer"
                        "fecha" => "2007"
                        "volumen" => "109"
                        "paginaInicial" => "25"
                        "paginaFinal" => "32"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17146782"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1002/cncr.22381"
                      "WWW" => array:1 [
                        "link" => "10&#46;1002&#47;cncr&#46;22381"
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                ]
              ]
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              "identificador" => "bib8"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Race and triple negative threats to breast cancer survival&#58; a population-based study in Atlanta&#44; GA"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              MJ Lund \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              KF Trivers \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              PL Porter \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              RJ Coates \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              B Leyland-Jones \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              OW Brawley \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1007/s10549-008-9926-3"
                      "Revista" => array:6 [
                        "tituloSerie" => "Breast Cancer Res Treat"
                        "fecha" => "2009"
                        "volumen" => "113"
                        "paginaInicial" => "357"
                        "paginaFinal" => "370"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18324472"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1007/s10549-008-9926-3"
                      "WWW" => array:1 [
                        "link" => "10&#46;1007&#47;s10549-008-9926-3"
                      ]
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                  ]
                ]
              ]
            ]
            8 => array:3 [
              "identificador" => "bib9"
              "etiqueta" => "9"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Variation in breast cancer subtypes with age and race&#47;ethnicity"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => """
                              CA Parise \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              KR Bauer \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              V Caggiano \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:2 [
                        "tituloSerie" => "Crit Rev Oncol Hematol"
                        "fecha" => "2009"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            9 => array:3 [
              "identificador" => "bib10"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Descriptive analysis of estrogen receptor &#40;ER&#41;-negative&#44; progesterone receptor &#40;PR&#41;-negative&#44; and HER2-negative invasive breast cancer&#44; the so-called triple-negative phenotype&#58; a population-based study from the California cancer Registry"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => """
                              KR Bauer \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              M Brown \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              RD Cress \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              CA Parise \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              V Caggiano \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Cancer"
                        "fecha" => "2007"
                        "volumen" => "109"
                        "paginaInicial" => "1721"
                        "paginaFinal" => "1728"
                      ]
                    ]
                    1 => array:1 [
                      "WWW" => array:1 [
                        "link" => "10&#46;1002&#47;cncr&#46;22618"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            10 => array:3 [
              "identificador" => "bib11"
              "etiqueta" => "11"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Expression of epidermal growth factor receptor in relation to BRCA1 status&#44; basal-like markers and prognosis in breast cancer"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              JB Arnes \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              LR Begin \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              I Stefansson \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              JS Brunet \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              TO Nielsen \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              WD Foulkes \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1136/jcp.2008.056291"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Pathol"
                        "fecha" => "2009"
                        "volumen" => "62"
                        "paginaInicial" => "139"
                        "paginaFinal" => "146"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18682421"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1136/jcp.2008.056291"
                      "WWW" => array:1 [
                        "link" => "10&#46;1136&#47;jcp&#46;2008&#46;056291"
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                  ]
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              ]
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            11 => array:3 [
              "identificador" => "bib12"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Immunohistochemical and clinical characterization of the basal-like subtype of invasive breast carcinoma"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              TO Nielsen \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              FD Hsu \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              K Jensen \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              M Cheang \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              G Karaca \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              Z Hu \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1158/1078-0432.CCR-04-0220"
                      "Revista" => array:6 [
                        "tituloSerie" => "Clin Cancer Res"
                        "fecha" => "2004"
                        "volumen" => "10"
                        "paginaInicial" => "5367"
                        "paginaFinal" => "5374"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15328174"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1158/1078-0432.CCR-04-0220"
                      "WWW" => array:1 [
                        "link" => "10&#46;1158&#47;1078-0432&#46;CCR-04-0220"
                      ]
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                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib13"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Is &#39;basal-like&#8217; carcinoma of the breast a distinct clinicopathological entity&#63; A critical review with cautionary notes"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => """
                              F Moinfar \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1159/000123850"
                      "Revista" => array:6 [
                        "tituloSerie" => "Pathobiology"
                        "fecha" => "2008"
                        "volumen" => "75"
                        "paginaInicial" => "119"
                        "paginaFinal" => "131"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18544967"
                            "web" => "Medline"
                          ]
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                      ]
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                    1 => array:2 [
                      "doi" => "10.1159/000123850"
                      "WWW" => array:1 [
                        "link" => "10&#46;1159&#47;000123850"
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                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib14"
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              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Invasive ductal carcinoma of the breast with the &#8220;triple-negative&#8221; phenotype&#58; prognostic implications of EGFR immunoreactivity"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              G Viale \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              N Rotmensz \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              P Maisonneuve \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              L Bottiglieri \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              E Montagna \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              A Luini \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1007/s10549-008-0206-z"
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                        "tituloSerie" => "Breast Cancer Res Treat"
                        "fecha" => "2009"
                        "volumen" => "116"
                        "paginaInicial" => "317"
                        "paginaFinal" => "328"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18839307"
                            "web" => "Medline"
                          ]
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                    1 => array:2 [
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              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "American Society of Clinical Oncology&#47;College of American Pathologists guideline recommendations for human epidermal growth factor receptor 2 testing in breast cancer"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              AC Wolff \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              ME Hammond \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              JN Schwartz \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              KL Hagerty \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              DC Allred \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              RJ Cote \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1200/JCO.2006.09.2775"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Oncol"
                        "fecha" => "2007"
                        "volumen" => "25"
                        "paginaInicial" => "118"
                        "paginaFinal" => "145"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17159189"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
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                        "link" => "10&#46;1200&#47;JCO&#46;2006&#46;09&#46;2775"
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                ]
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            ]
            15 => array:3 [
              "identificador" => "bib16"
              "etiqueta" => "16"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Estrogen receptor-positive breast carcinomas in younger women are different from those of older women&#58; A pathological and immunohistochemical study"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => """
                              LM Bacchi \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              M Corpa \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              PP Santos \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              CE Bacchi \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              FM Carvalho \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1016/j.breast.2010.01.002"
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                        "tituloSerie" => "Breast"
                        "fecha" => "2010"
                        "volumen" => "19"
                        "paginaInicial" => "137"
                        "paginaFinal" => "141"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20117934"
                            "web" => "Medline"
                          ]
                        ]
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                    ]
                    1 => array:2 [
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                        "link" => "10&#46;1016&#47;j&#46;breast&#46;2010&#46;01&#46;002"
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                    ]
                  ]
                ]
              ]
            ]
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              "identificador" => "bib17"
              "etiqueta" => "17"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The clinicopathologic characteristics and prognostic significance of triple-negativity in node-negative breast cancer"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:5 [
                            0 => """
                              J Rhee \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              SW Han \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              DY Oh \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              JH Kim \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              SA Im \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1186/1471-2407-8-307"
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                        "tituloSerie" => "BMC Cancer"
                        "fecha" => "2008"
                        "volumen" => "8"
                        "paginaInicial" => "307"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18947390"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1186/1471-2407-8-307"
                      "WWW" => array:1 [
                        "link" => "10&#46;1186&#47;1471-2407-8-307"
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                    ]
                  ]
                ]
              ]
            ]
            17 => array:3 [
              "identificador" => "bib18"
              "etiqueta" => "18"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Triple negative breast carcinoma and the basal phenotype&#58; from expression profiling to clinical practice"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => """
                              LK Diaz \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              VL Cryns \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              WF Symmans \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              N Sneige \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
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                        "link" => array:1 [
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              "etiqueta" => "19"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Specific morphological features predictive for the basal phenotype in grade 3 invasive ductal carcinoma of breast"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              LG Fulford \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              DF Easton \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              JS Reis-Filho \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              A Sofronis \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              CE Gillett \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              SR Lakhani \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Histopathology"
                        "fecha" => "2006"
                        "volumen" => "49"
                        "paginaInicial" => "22"
                        "paginaFinal" => "34"
                      ]
                    ]
                    1 => array:1 [
                      "WWW" => array:1 [
                        "link" => "10&#46;1111&#47;j&#46;1365-2559&#46;2006&#46;02453&#46;x"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            19 => array:3 [
              "identificador" => "bib20"
              "etiqueta" => "20"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "p63&#44; cytokeratin 5&#44; and P-cadherin&#58; three molecular markers to distinguish basal phenotype in breast carcinomas"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:5 [
                            0 => """
                              I Matos \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              R Dufloth \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              M Alvarenga \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              LC Zeferino \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              F Schmitt \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1007/s00428-005-0010-7"
                      "Revista" => array:6 [
                        "tituloSerie" => "Virchows Arch"
                        "fecha" => "2005"
                        "volumen" => "447"
                        "paginaInicial" => "688"
                        "paginaFinal" => "694"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16012853"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1007/s00428-005-0010-7"
                      "WWW" => array:1 [
                        "link" => "10&#46;1007&#47;s00428-005-0010-7"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            20 => array:3 [
              "identificador" => "bib21"
              "etiqueta" => "21"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Phenotypic evaluation of the basal-like subtype of invasive breast carcinoma"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              CA Livasy \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              G Karaca \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              R Nanda \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              MS Tretiakova \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              OI Olopade \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              DT Moore \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1038/modpathol.3800528"
                      "Revista" => array:6 [
                        "tituloSerie" => "Mod Pathol"
                        "fecha" => "2006"
                        "volumen" => "19"
                        "paginaInicial" => "264"
                        "paginaFinal" => "271"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16341146"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1038/modpathol.3800528"
                      "WWW" => array:1 [
                        "link" => "10&#46;1038&#47;modpathol&#46;3800528"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            21 => array:3 [
              "identificador" => "bib22"
              "etiqueta" => "22"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The phenotypic spectrum of basal-like breast cancers&#58; a critical appraisal"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => """
                              O Fadare \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              FA Tavassoli \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1097/PAP.0b013e31814b26fe"
                      "Revista" => array:6 [
                        "tituloSerie" => "Adv Anat Pathol"
                        "fecha" => "2007"
                        "volumen" => "14"
                        "paginaInicial" => "358"
                        "paginaFinal" => "373"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/17717437"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1097/PAP.0b013e31814b26fe"
                      "WWW" => array:1 [
                        "link" => "10&#46;1097&#47;PAP&#46;0b013e31814b26fe"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            22 => array:3 [
              "identificador" => "bib23"
              "etiqueta" => "23"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Basal carcinoma of the breast revisited&#58; an old entity with new interpretations"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => """
                              E Korsching \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              SS Jeffrey \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              W Meinerz \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              T Decker \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              W Boecker \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              H Buerger \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1136/jcp.2008.055475"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Pathol"
                        "fecha" => "2008"
                        "volumen" => "61"
                        "paginaInicial" => "553"
                        "paginaFinal" => "560"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18326009"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1136/jcp.2008.055475"
                      "WWW" => array:1 [
                        "link" => "10&#46;1136&#47;jcp&#46;2008&#46;055475"
                      ]
                    ]
                  ]
                ]
              ]
            ]
            23 => array:3 [
              "identificador" => "bib24"
              "etiqueta" => "24"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Morphological and immunophenotypic analysis of breast carcinomas with basal and myoepithelial differentiation"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              EA Rakha \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              TC Putti \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              DM Abd El-Rehim \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              C Paish \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              AR Green \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              DG Powe \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1002/path.1916"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Pathol"
                        "fecha" => "2006"
                        "volumen" => "208"
                        "paginaInicial" => "495"
                        "paginaFinal" => "506"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16429394"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1002/path.1916"
                      "WWW" => array:1 [
                        "link" => "10&#46;1002&#47;path&#46;1916"
                      ]
                    ]
                  ]
                ]
              ]
            ]
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              "identificador" => "bib25"
              "etiqueta" => "25"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Breast cancer in women under 40years of age&#58; A series of 57 cases from Northern Ireland"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => """
                              B McAree \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              ME O&#39;Donnell \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              A Spence \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              TF Lioe \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              DT McManus \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              RA Spence \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1016/j.breast.2009.12.002"
                      "Revista" => array:6 [
                        "tituloSerie" => "Breast"
                        "fecha" => "2010"
                        "volumen" => "19"
                        "paginaInicial" => "97"
                        "paginaFinal" => "104"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20060718"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1016/j.breast.2009.12.002"
                      "WWW" => array:1 [
                        "link" => "10&#46;1016&#47;j&#46;breast&#46;2009&#46;12&#46;002"
                      ]
                    ]
                  ]
                ]
              ]
            ]
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              "identificador" => "bib26"
              "etiqueta" => "26"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Basal cytokeratin and epidermal growth factor receptor expression are not predictive of BRCA1 mutation status in women with triple-negative breast cancers"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              LC Collins \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              A Martyniak \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              MJ Kandel \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              ZK Stadler \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              S Masciari \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              A Miron \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1097/PAS.0b013e31819c1c93"
                      "Revista" => array:6 [
                        "tituloSerie" => "Am J Surg Pathol"
                        "fecha" => "2009"
                        "volumen" => "33"
                        "paginaInicial" => "1093"
                        "paginaFinal" => "1097"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19390427"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1097/PAS.0b013e31819c1c93"
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                        "link" => "10&#46;1097&#47;PAS&#46;0b013e3181b7cb7a"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Epidermal growth factor receptor expression in breast cancer association with biologic phenotype and clinical outcomes"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => """
                              MF Rimawi \n
                              \t\t\t\t\t\t\t\t
                              """
                            1 => """
                              PB Shetty \n
                              \t\t\t\t\t\t\t\t
                              """
                            2 => """
                              HL Weiss \n
                              \t\t\t\t\t\t\t\t
                              """
                            3 => """
                              R Schiff \n
                              \t\t\t\t\t\t\t\t
                              """
                            4 => """
                              CK Osborne \n
                              \t\t\t\t\t\t\t\t
                              """
                            5 => """
                              GC Chamness \n
                              \t\t\t\t\t\t\t\t
                              """
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:2 [
                    0 => array:2 [
                      "doi" => "10.1002/cncr.24816"
                      "Revista" => array:6 [
                        "tituloSerie" => "Cancer"
                        "fecha" => "2010"
                        "volumen" => "116"
                        "paginaInicial" => "1234"
                        "paginaFinal" => "1242"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20082448"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                    1 => array:2 [
                      "doi" => "10.1002/cncr.24816"
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        "texto" => "<p id="para120" class="elsevierStylePara elsevierViewall">This work was supported by FAPESP &#40;Funda&#231;&#227;o de Amparo a Pesquisa do Estado de Sao Paulo&#41;&#58; grant &#35;2007-03139-9 &#40;FMC&#41; and scientific initiation scholarship &#35;07&#47;51613-1 &#40;LMB&#41;&#46;</p>"
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Article information

ISSN: 18075932
Original language: English

DOI:10.1590/S1807-59322010001000019

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Year/Month	Html	Pdf	Total

2024 November	4	0	4
2024 October	40	27	67
2024 September	30	42	72
2024 August	35	40	75
2024 July	35	40	75
2024 June	34	16	50
2024 May	55	22	77
2024 April	43	12	55
2024 March	49	23	72
2024 February	34	41	75
2024 January	29	19	48
2023 December	21	26	47
2023 November	26	28	54
2023 October	33	29	62
2023 September	24	31	55
2023 August	18	10	28
2023 July	7	15	22
2023 June	23	13	36
2023 May	39	4	43
2023 April	38	0	38
2023 March	24	13	37
2023 February	27	3	30
2023 January	20	2	22
2022 December	14	0	14

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