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"https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/13052758?idApp=UINPBA00004N" ] ] "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/13052329?idApp=UINPBA00004N" "url" => "/0213005X/0000002100000009/v0_201607121151/13052329/v0_201607121151/es/main.assets" ] "itemAnterior" => array:14 [ "pii" => "13052328" "issn" => "0213005X" "estado" => "S300" "fechaPublicacion" => "2003-11-01" "documento" => "article" "subdocumento" => "fla" "cita" => "Enferm Infecc Microbiol Clin. 2003;21:469-71" "abierto" => array:3 [ "ES" => false "ES2" => false "LATM" => false ] "gratuito" => false "lecturas" => array:2 [ "total" => 3236 "formatos" => array:3 [ "EPUB" => 12 "HTML" => 2747 "PDF" => 477 ] ] "es" => array:9 [ "idiomaDefecto" => true "titulo" => "¿Tienen utilidad las técnicas moleculares para la vigilancia y el control de la aspergilosis?" "tienePdf" => "es" "tieneTextoCompleto" => "es" "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "469" "paginaFinal" => "471" ] ] "titulosAlternativos" => array:1 [ "en" => array:1 [ "titulo" => "Are molecular techniques useful in aspergillosis surveillance and control?" ] ] "contieneTextoCompleto" => array:1 [ "es" => true ] "contienePdf" => array:1 [ "es" => true ] "autores" => array:1 [ 0 => array:2 [ "autoresLista" => "Manuel Cuenca-Estrella, Emilia Mellado" "autores" => array:2 [ 0 => array:2 [ "nombre" => "Manuel" "apellidos" => "Cuenca-Estrella" ] 1 => array:2 [ "nombre" => "Emilia" "apellidos" => "Mellado" ] ] ] ] ] "idiomaDefecto" => "es" "EPUB" => "https://multimedia.elsevier.es/PublicationsMultimediaV1/item/epub/13052328?idApp=UINPBA00004N" "url" => "/0213005X/0000002100000009/v0_201607121151/13052328/v0_201607121151/es/main.assets" ] "en" => array:14 [ "idiomaDefecto" => true "titulo" => "Combined use of Random Amplified Polymorphic DNA (RAPD) and touchdown Polymerase Chain Reaction (PCR) for Aspergillus fumigatus epidemiologic studies" "tieneTextoCompleto" => true "paginas" => array:1 [ 0 => array:2 [ "paginaInicial" => "472" "paginaFinal" => "476" ] ] "autores" => array:1 [ 0 => array:3 [ "autoresLista" => "Mercedes Treviño-Castellano, Sonia Rodríguez-Novoa, José Llovo-Taboada, Ángeles García-Zabarte, Carlos García-Riestra, Benito José Regueiro-García" "autores" => array:6 [ 0 => array:3 [ "nombre" => "Mercedes" "apellidos" => "Treviño-Castellano" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "affa" ] ] ] 1 => array:3 [ "nombre" => "Sonia" "apellidos" => "Rodríguez-Novoa" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "affa" ] ] ] 2 => array:3 [ "nombre" => "José" "apellidos" => "Llovo-Taboada" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "affa" ] ] ] 3 => array:3 [ "nombre" => "Ángeles" "apellidos" => "García-Zabarte" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "affa" ] ] ] 4 => array:3 [ "nombre" => "Carlos" "apellidos" => "García-Riestra" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "affa" ] ] ] 5 => array:3 [ "nombre" => "Benito José" "apellidos" => "Regueiro-García" "referencia" => array:1 [ 0 => array:2 [ "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "affa" ] ] ] ] "afiliaciones" => array:1 [ 0 => array:3 [ "entidad" => "Servicio de Microbiología. Complejo Hospitalario Universitario de Santiago de Compostela (CHUS). A Coruña. España." "etiqueta" => "<span class="elsevierStyleSup">a</span>" "identificador" => "affa" ] ] ] ] "titulosAlternativos" => array:1 [ "en" => array:1 [ "titulo" => "Uso combinado de amplificacion aleatoria de polimorfismo del ADN (RAPD) y reacción en cadena de la polimerasa (touchdown PCR) en el estudio epidemiológico de Aspergillus fumigatus" ] ] "textoCompleto" => "<p class="elsevierStylePara">Introduction</p><p class="elsevierStylePara"><span class="elsevierStyleItalic">Aspergillus fumigatus</span>, a filamentous fungus which causes several pulmonary conditions such as allergic bronchopulmonary aspergillosis, aspergilloma and invasive aspergillosis, has emerged as a major problem in immunosupressed patients<span class="elsevierStyleSup">1</span>. Nosocomial outbreaks of aspergillosis are being more frequent, and identification of the isolates may be delayed because of waiting for diagnostically appropiate structures. Furthermore, inexperience in microscopy may lead to misidentification.</p><p class="elsevierStylePara">Randomly amplified polymorphic DNA (RAPD) analysis assays DNA sequence variation in short regions using one short primer and a low annealing temperature to generate several fragments in one amplification reaction<span class="elsevierStyleSup">2</span>. Moreover, RAPD analysis is technically simple and often detects variation among isolates that are invariant with Restriction Fragment Length Polymorphism (RFLP) analysis<span class="elsevierStyleSup">3,4</span>. However, this method is gradually being abandoned because of poor reproducibility<span class="elsevierStyleSup">5</span>. Recently, Ellinghaus et al. have communicated increased efficiency and reproducibility of arbitrarily primed PCR by prolonging ramp times<span class="elsevierStyleSup">6</span>.</p><p class="elsevierStylePara">Otherwise, touchdown PCR with Z19-276 and Z19-660 primers described by Brandt et al.<span class="elsevierStyleSup">7</span> is a useful tool in molecular identification of <span class="elsevierStyleItalic"> Aspergillus fumigatus</span>. This shortens the time, substantially, needed for fungal identification.</p><p class="elsevierStylePara">The present report describes the use of RAPD analysis as a reproducible genotyping method for <span class="elsevierStyleItalic">A. fumigatus</span>.</p><p class="elsevierStylePara">Methods</p><p class="elsevierStylePara">A total of 16 fungal strains isolated from the clinical and hospital environment were tested in this study. American Type Culture Collection (ATCC) 13073 <span class="elsevierStyleItalic">A. fumigatus</span> and ATCC 1004 <span class="elsevierStyleItalic">A. niger</span> were used as positive and negative control, respectively. Isolates were stored at ­70 <span class="elsevierStyleSup">o</span>C in brain heart infusion broth containing 10% glycerol. Working stocks were mantained on Sabouraud agar medium at 4 <span class="elsevierStyleSup">o</span>C, and species identifications were made on the basis of standard morphological features<span class="elsevierStyleSup">8</span>.</p><p class="elsevierStylePara">DNA extraction</p><p class="elsevierStylePara">DNA was extracted by a rapid small-scale method that allows several samples to be processed simultaneously. Briefly, a Sabouraud-dextrose agar in a petri dish was inoculated with conidia and incubated at 37<span class="elsevierStyleSup">o</span>C during 24 to 48h. Mycelial growth was peeled off from the agar surface with a sterile scalpel and transferred to a 1.5 ml polypropylene screw-cap tube containing six glass beads. The tube was immersed in liquid nitrogen for 10 s and vortexed vigorously for 30s. Then ground fungal tissue was processed by E.Z.N.A. system (a purifying-column based method) (Omega Biotek, USA) according to the manufacturer´s instructions. The DNA concentrations were estimated spectrophotometrically.</p><p class="elsevierStylePara">Touchdown PCR</p><p class="elsevierStylePara">It was performed in a model 9600 DNA thermal cycler (Perkin-Elmer Corp., Applied Biosystems). PCR core reagents and polymerase were purchased from Perkin-Elmer. PCR reaction and amplification conditions were made as previously described<span class="elsevierStyleSup">7</span>. Two primers were chosen to amplify the 864-bp <span class="elsevierStyleItalic">A. fumigatus</span>-specific fragment: primer Z19-276 (5'-TTGATCTGGCCCTGGCTTGGG) and primer Z19-660 (5'-CAACATTGAAATCCAAGAGGC). Amplification reactions were made in 50 µ L volumes containing 1 x PCR buffer (Perkin-Elmer), 2.5 mM MgCl<span class="elsevierStyleInf">2</span>, 0.2 mM each deoxinucleotide triphosphate, 10 pmol each primer, 1 U AmpliTaq and 50 ng DNA. Amplification protocol was: 31 cycles of 1 minute at 94 ºC for denaturation, 1 minute at 65 ºC for the three begining cycles and, after, it was decreasing one grade each three cycles, and two grades from 59 ºC to 55 ºC, temperature used to perform the last 10 cycles. All cycles were finished with 1 minute at 72 ºC for elongation.</p><p class="elsevierStylePara">RAPD analysis</p><p class="elsevierStylePara">DNA typing by RAPD was assayed with two oligonucleotide primers, OPZ19 (5'-GTGCGAGCAA) and R108 (5'-GTATTGCCCT). Amplification reactions were made in 50 µ L volumes containing 50 mM KCl, 10 mM Tris-Cl (pH 8.0); 1.5 mM MgCl<span class="elsevierStyleInf">2</span>; 100 µM each dATP, dCTP, dTTP, and dGTP (Perkin-Elmer), 0.2 µM each primer?; 50 ng of genomic DNA; and 2.5 U of Taq polymerase (Perkin-Elmer). Amplifications were carried out by two different protocols. The first one at 1 cycle of 5 minutes at 95 <span class="elsevierStyleSup">o</span>C to denature followed by 45 cycles of 1 minute at 95 <span class="elsevierStyleSup">o</span>C, 1 minute at 34 <span class="elsevierStyleSup">o</span>C and 2 minutes at 72 <span class="elsevierStyleSup">o</span>C (temperature/time rate as minimal as possible) and, finally, 10-minutes final extension at 72 <span class="elsevierStyleSup"> o</span>C. The second one was performed prolonging ramp times drastically between annealing and extension temperatures, 5 and 7 minutes, respectively. Amplification products were fractionated by electrophoresis through 1.8% agarose NuSieve 3:1 (FMC Bioproducts, USA) gels run in 0.5x Tris-borate-EDTA in the presence of ethidium bromide (0.5 mg/ml), photographed and analyzed. Lanes corresponding to the same sample in different runs were compared by superimposing their intensity profiles (the Rf values in X axis and the pixel intensity values in Y axis) using Quantity one vs. 4.0 software (Bio-Rad, USA). Afterwards, the similarity coefficient (S) was calculated according to S= 2 N<span class="elsevierStyleInf">AB</span>/ N<span class="elsevierStyleInf">A</span> + N<span class="elsevierStyleInf">B</span><span class="elsevierStyleSup">2</span></p><p class="elsevierStylePara">Results</p><p class="elsevierStylePara">Phenotypical identification of the fungal strains</p><p class="elsevierStylePara">A total of 16 fungal strains were isolated by culture techniques. Four <span class="elsevierStyleItalic">A. fumigatus</span> strains (strains number 1, 2, 3, 5) and 1 <span class="elsevierStyleItalic">A. niger</span> strain from clinical source. The following strains were recovered from the hospital environment: 5 <span class="elsevierStyleItalic">A. fumigatus</span> (strains number 7, 8, 9, 11, 13), 2 <span class="elsevierStyleItalic">A. niger</span> (strains number 14, 16), 1 <span class="elsevierStyleItalic">Emericella quadrilineata</span> (strains number 10), 1 <span class="elsevierStyleItalic">Emericella nidulans</span> (strain number 15), 1 <span class="elsevierStyleItalic">Neosartorya pseudofischeri</span> (strain number 4) and 1 <span class="elsevierStyleItalic">Eurotium repens</span> (strain number 17).</p><p class="elsevierStylePara">Touchdown PCR</p><p class="elsevierStylePara">A 864 bp amplification product was obtained with the touchdown PCR protocol using Z19-276/Z19-660 primer pair from fungal strains number 1, 2, 3, 5, 7, 8, 9, 11, 13, and <span class="elsevierStyleItalic">A. fumigatus</span> from ATCC according, exactly, to the strains identified as <span class="elsevierStyleItalic">A. fumigatus</span> by phenotypical methods. No amplification band was obtained for the other strains which were morphologically classified as other different species than <span class="elsevierStyleItalic">A. fumigatus</span> (figure 1).</p><p class="elsevierStylePara"><img src="28v21n9-13052758tab01.gif"></img></p><p class="elsevierStylePara"><span class="elsevierStyleBold">Figure 1.</span> DNA from clinical and environmental fungal isolates amplified with primers Z19-276 and Z19-660 by touchdown protocol. Lanes: <span class="elsevierStyleBold">1</span>, PCR marker (base pair); <span class="elsevierStyleBold">2, 3, 4, 6:</span><span class="elsevierStyleItalic">A. fumigatus</span> from clinical samples (strains nº 1, 2, 3, 5); <span class="elsevierStyleBold">8, 9, 10, 12, 14:</span><span class="elsevierStyleItalic">A. fumigatus</span> from environmental samples (strains nº 7, 8, 9, 11, 13); <span class="elsevierStyleBold">11</span>: <span class="elsevierStyleItalic">E. quadrilineata</span> (strain nº 10), <span class="elsevierStyleBold">13</span>: <span class="elsevierStyleItalic">A. niger</span>, (ATCC1004) (strain nº 12); <span class="elsevierStyleBold">15, 17:</span><span class="elsevierStyleItalic">A. niger</span> de origen ambiental (cepas nº 14, 16); <span class="elsevierStyleBold">16:</span><span class="elsevierStyleItalic">E. nidulans</span> (cepa nº 15); <span class="elsevierStyleBold">18</span>: <span class="elsevierStyleItalic">E. repens</span> (cepa nº 17); <span class="elsevierStyleBold">19:</span><span class="elsevierStyleItalic">A. fumigatus</span> from ATCC.</p><p class="elsevierStylePara">RAPD analysis</p><p class="elsevierStylePara">DNAs from a set of isolates comprising clinical and environmental <span class="elsevierStyleItalic">A. fumigatus</span> strains were screened with OPZ19 and R108 primers. The most discriminatory power was obtained for R108 primer which rendered six different types of patterns of bands (figure 2). The reproducibility and number of bands in RAPD analysis with R108 primer increased prolonging ramp times (table 1, figure 3). On the contrary, RAPD with OPZ19 primer had an acceptable reproducibility but very little resolution showing two different pattern of bands only, at any of the tested assay conditions (figure 4).</p><p class="elsevierStylePara"><img src="28v21n9-13052758tab02.gif"></img></p><p class="elsevierStylePara"><img src="28v21n9-13052758tab03.gif"></img></p><p class="elsevierStylePara"><img src="28v21n9-13052758tab04.gif"></img></p><p class="elsevierStylePara"><span class="elsevierStyleBold">Figure 2.</span> RAPD patterns produced with R108 primer. A: Ramp time = 0 minutes. Lanes: <span class="elsevierStyleBold">1</span>: PCR marker (base pair), lanes <span class="elsevierStyleBold">2-10:</span><span class="elsevierStyleItalic">A. fumigatus</span> (strains nº 1, 2, 3, 5, 7, 8, 9, 11, 13), <span class="elsevierStyleBold"> 11:</span><span class="elsevierStyleItalic">A. fumigatus</span> from ATCC. B: Ramp time = 5 minutes. Lanes: <span class="elsevierStyleBold">1-9</span>: <span class="elsevierStyleItalic">A. fumigatus</span> (strain nº 1, 2, 3, 5, 7, 8, 9, 11, 13); <span class="elsevierStyleBold">10<span class="elsevierStyleItalic">:</span></span><span class="elsevierStyleItalic">A. fumigatus</span> from ATCC; <span class="elsevierStyleBold">11:</span> Size marker (bp). C: Ramp time = 7 minutes. Lanes distribution as in figure 2 A.</p><p class="elsevierStylePara"><img src="28v21n9-13052758tab05.gif"></img></p><p class="elsevierStylePara"><img src="28v21n9-13052758tab06.gif"></img></p><p class="elsevierStylePara"><span class="elsevierStyleBold">Figure 3.</span> Patterns of bands obtained in different runs with different DNA extractions. A and B: Two different runs using the first DNA extraction. Figures C and D: Two different runs using the second DNA extraction. Lanes: <span class="elsevierStyleBold">1:</span> Size marker; <span class="elsevierStyleBold">2-10:</span><span class="elsevierStyleItalic">A. fumigatus</span> (strain nº 1, 2, 3, 5, 7, 8, 9, 11, 13), <span class="elsevierStyleBold"> 11:</span><span class="elsevierStyleItalic">A. fumigatus</span> from ATCC.</p><p class="elsevierStylePara"><img src="28v21n9-13052758tab07.gif"></img></p><p class="elsevierStylePara"><span class="elsevierStyleBold">Figure 4.</span> DNA from <span class="elsevierStyleItalic">A. fumigatus</span> strains amplified by RAPD-PCR using the OPZ-19 primer. Lanes: <span class="elsevierStyleBold">1:</span> PCR marker (bp); <span class="elsevierStyleBold">2-10:</span><span class="elsevierStyleItalic">A. fumigatus</span> (strains number 1, 2, 3, 5, 7, 8, 9, 11, 13); 11: <span class="elsevierStyleItalic"> A. fumigatus</span> from ATCC.</p><p class="elsevierStylePara">Discussion</p><p class="elsevierStylePara">By using touchdown annealing conditions, the Z19-276/660 primer-probe combination successfully amplified and detected DNAs from all the strains that were morphologically identified as <span class="elsevierStyleItalic">A. fumigatus.</span> So, this result confirms the great utility for accurate and timely identification of <span class="elsevierStyleItalic">A. fumigatus</span> from clinical and environmental samples. This detection is extremily important for the diagnosis and management of the diseases as well as for surveillance and epidemiologic studies. However, some uncommon varieties <span class="elsevierStyleItalic">as A. fumigatus var. sclerotium</span> and non pigmented strains may show faint or no reactivity under these conditions<span class="elsevierStyleSup">7</span>.</p><p class="elsevierStylePara">Among several techniques that have been applied successfully to study fungal epidemiology, RAPD analysis is the most technically simple and often detects variation between isolates that are invariant with RFLP analysis<span class="elsevierStyleSup">10-12</span>. However, its poor reproducibility has been considered to be a serious disadvantage. R108, a primer used successfully in RAPD analysis of <span class="elsevierStyleItalic">A. fumigatus</span> by other authors<span class="elsevierStyleSup">4,13</span>, demonstrated a high discriminatory power, which is consistent with the results obtained by other authors<span class="elsevierStyleSup">4,14</span>. Only two clinical strains were not distinguished by R108 protocol. These ones were from patients who stayed at the same operating theater and postsurgery care floor, at the same time. Therefore, they were, probably, infected by the same strain.</p><p class="elsevierStylePara">Although it is advisable that RAPD analysis be made with several primers, the use, only, of one of them with high discriminatory power, may be enough to manage the outbreak, avoiding more assays money and time-consuming<span class="elsevierStyleSup">15</span>. In our study, prolonged ramp times between the annealing and the extension temperatures lead to an important increase in reproducibility of the patterns of bands obtained with R108 primer, according to the results obtained by P. Ellinghaus et al. with <span class="elsevierStyleItalic">Candida sp.</span> genome<span class="elsevierStyleSup">6</span>. Also, the number, yield and reproducibility of the DNA fingerprint obtained was improved, significantly. A potential mechanism might be that the lower heating rates stabilize the primer/template complexes by avoiding premature detachment of the primer from the template. Otherwise, RAPD with OPZ19, a primer reactive with all strains of <span class="elsevierStyleItalic">A. fumigatus</span>, except non pigmented variants, had acceptable repetitivity with all the ramp times assayed, perhaps related with their own sequence because it is known that reproducibility and intensity of the bands in a fingerprint should be a function of several parameters including primer length and primer sequence<span class="elsevierStyleSup">16</span>. However, prolonged ramp times reported an increase in number and yield of the DNA bands obtained.</p><p class="elsevierStylePara">In conclusion, RAPD analysis is a truly rapid and reliable tool in DNA fingerprinting. Patterns may be easier to repeat and interpret when drastically prolonged ramp times between annealing and extension are used. Combining touchdown PCR and RAPD analysis is a sensible and accurate method for epidemiologic studies of clinical outbreaks of <span class="elsevierStyleItalic">A. fumigatus</span> making use of the habitual techniques available in a current clinical microbiology laboratory.</p><p class="elsevierStylePara"> Acknowledgments</p><p class="elsevierStylePara">We thank A.M. Commons for translation review of the manuscript.</p>" "pdfFichero" => "28v21n9a13052758pdf001.pdf" "tienePdf" => true "PalabrasClave" => array:2 [ "en" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Keywords" "identificador" => "xpalclavsec693625" "palabras" => array:4 [ 0 => "Aspergillus fumigatus" 1 => "RAPD" 2 => "Touchdown PCR" 3 => "Aspergillus fingerprint" ] ] ] "es" => array:1 [ 0 => array:4 [ "clase" => "keyword" "titulo" => "Palabras clave" "identificador" => "xpalclavsec693624" "palabras" => array:4 [ 0 => "Aspergillus fumigatus" 1 => "RAPD" 2 => "Touchdown PCR" 3 => "Aspergillus fingerprint" ] ] ] ] "tieneResumen" => true "resumen" => array:2 [ "en" => array:1 [ "resumen" => "Introduction. Aspergillus fumigatus is a filamentous fungus that acts as an opportunistic pathogen and has emerged as a major problem in immunosuppressed patients. Nosocomial outbreaks of aspergillosis are becoming more frequent, but their identification and epidemiological characterization is slow and difficult. Objective. Description of a fast, sensitive, specific method to identify and fingerprint A. fumigatus using methodology available in clinical laboratories. Methods. We studied several strains of A. fumigatus isolated from patients with invasive aspergillosis (n 5 4), the hospital environment (n 5 5) and reference cultures (n 5 1), as well as other close phylogenetic fungal species from patients (n 5 1), hospital environment (n 5 6) and reference cultures (n 5 1). A. fumigatus was identified by both touchdown PCR and conventional phenotyping methods. Genotyping was performed with random amplification of polymorphic DNA (RAPD) analysis, comparing the results from two primers (OPZ-19 and R-108) and different amplification protocols with regard to band resolution and reproducibility. Results. Touchdown PCR and phenotype results were identical. Best RAPD results were obtained with the R-108 primer and considerably longer ramp times between annealing and extension. Conclusion. RAPD analysis is a fast, reliable tool for DNA fingerprinting. Patterns may be easier to repeat and interpret when longer ramp times are used. Touchdown PCR combined with RAPD analysis is a sensitive, accurate method for managing clinical outbreaks of Aspergillus fumigatus." ] "es" => array:1 [ "resumen" => "Introducción. Aspergillus fumigatus es un hongo filamentoso que se comporta como patógeno oportunista y constituye una de las complicaciones infecciosas más importantes en los pacientes inmunocomprometidos. Los brotes nosocomiales de aspergilosis son cada vez más frecuentes, pero su identificación y caracterización epidemiológica es lenta y laboriosa. Objetivo. Describir un método rápido, sensible y específico para la identificación de A. fumigatus y su caracterización genotípica dentro de las posibilidades diagnósticas habituales en un laboratorio clínico de microbiología. Métodos. Se utilizaron cepas de A. fumigatus procedentes de pacientes con aspergilosis invasivas (n 5 4), medio ambiente hospitalario (n 5 5) y colecciones de referencia (n 5 1), así como otras especies fúngicas filogenéticamente próximas aisladas de pacientes (n 5 1), del medio hospitalario (n 5 6) o de colecciones de referencia (n 5 1). La identificación de A. fumigatus se realizó tanto por métodos fenotípicos clásicos como mediante touchdown PCR (reacción en cadena de la polimerasa). La caracterización genotípica se llevó a cabo por RAPD (polimorfismo derivado de la amplificación aleatoria de ADN), comparando distintos protocolos de amplificación y tipos de primer (OPZ-19 y R-108) en relación con su poder resolutivo y reproducibilidad. Resultados. Los resultados de la identificación fenotípica y molecular coincidieron plenamente. La caracterización molecular por RAPD presentó los mejores resultados, en cuanto a reproducibilidad y resolución se refiere, con el primer R-108 y tiempo prolongado de transición entre hibridación y elongación. Conclusión. El análisis por RAPD es un método seguro y rápido para la caracterización genotípica de A. fumigatus cuyos patrones de bandas son fáciles de interpretar y reproducir cuando se prolonga drásticamente el tiempo de transición entre la hibridación y la extensión. El uso combinado de touchdown PCR y análisis por RAPD constituye un método sensible y exacto para la resolución de brotes nosocomiales por A. fumigatus." ] ] "multimedia" => array:14 [ 0 => array:8 [ "identificador" => "tbl1" "etiqueta" => "Figure 1" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "tabla" => array:1 [ "tablatextoimagen" => array:1 [ 0 => array:1 [ "tablaImagen" => array:1 [ 0 => array:4 [ "imagenFichero" => "28v21n9-13052758tab01.gif" "imagenAlto" => 309 "imagenAncho" => 730 "imagenTamanyo" => 25468 ] ] ] ] ] "descripcion" => array:1 [ "en" => "DNA from clinical and environmental fungal isolates amplified with primers Z19-276 and Z19-660 by touchdown protocol. Lanes: 1, PCR marker (base pair); 2, 3, 4, 6: A. fumigatus from clinical samples (strains nº 1, 2, 3, 5); 8, 9, 10, 12, 14: A. fumigatus from environmental samples (strains nº 7, 8, 9, 11, 13); 11: E. quadrilineata (strain nº 10), 13: A. niger, (ATCC1004) (strain nº 12); 15, 17: A. niger de origen ambiental (cepas nº 14, 16); 16: E. nidulans (cepa nº 15); 18: E. repens (cepa nº 17); 19: A. fumigatus from ATCC." ] ] 1 => array:8 [ "identificador" => "tbl2" "etiqueta" => "TABLE 1" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "tabla" => array:1 [ "tablatextoimagen" => array:1 [ 0 => array:1 [ "tablaImagen" => array:1 [ 0 => array:4 [ "imagenFichero" => "28v21n9-13052758tab02.gif" "imagenAlto" => 171 "imagenAncho" => 416 "imagenTamanyo" => 6477 ] ] ] ] ] "descripcion" => array:1 [ "en" => "Similarity coefficients values between different runs at different ramp time RAPD protocols" ] ] 2 => array:8 [ "identificador" => "tbl3" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "tabla" => array:1 [ "tablatextoimagen" => array:1 [ 0 => array:1 [ "tablaImagen" => array:1 [ 0 => array:4 [ "imagenFichero" => "28v21n9-13052758tab03.gif" "imagenAlto" => 208 "imagenAncho" => 750 "imagenTamanyo" => 36338 ] ] ] ] ] "descripcion" => array:1 [ "en" => "RAPD patterns produced with R108 primer. A: Ramp time = 0 minutes. Lanes: 1: PCR marker (base pair), lanes 2-10: A. fumigatus (strains nº 1, 2, 3, 5, 7, 8, 9, 11, 13), 11: A. fumigatus from ATCC. B: Ramp time = 5 minutes. Lanes: 1-9: A. fumigatus (strain nº 1, 2, 3, 5, 7, 8, 9, 11, 13); 10: A. fumigatus from ATCC; 11: Size marker (bp). C: Ramp time = 7 minutes. Lanes distribution as in figure 2 A." ] ] 3 => array:8 [ "identificador" => "tbl4" "etiqueta" => "Figure 2" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "tabla" => array:1 [ "tablatextoimagen" => array:1 [ 0 => array:1 [ "tablaImagen" => array:1 [ 0 => array:4 [ "imagenFichero" => "28v21n9-13052758tab04.gif" "imagenAlto" => 593 "imagenAncho" => 730 "imagenTamanyo" => 51347 ] ] ] ] ] "descripcion" => array:1 [ "en" => "RAPD patterns produced with R108 primer. A: Ramp time = 0 minutes. Lanes: 1: PCR marker (base pair), lanes 2-10: A. fumigatus (strains nº 1, 2, 3, 5, 7, 8, 9, 11, 13), 11: A. fumigatus from ATCC. B: Ramp time = 5 minutes. Lanes: 1-9: A. fumigatus (strain nº 1, 2, 3, 5, 7, 8, 9, 11, 13); 10: A. fumigatus from ATCC; 11: Size marker (bp). C: Ramp time = 7 minutes. Lanes distribution as in figure 2 A." ] ] 4 => array:8 [ "identificador" => "tbl5" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "tabla" => array:1 [ "tablatextoimagen" => array:1 [ 0 => array:1 [ "tablaImagen" => array:1 [ 0 => array:4 [ "imagenFichero" => "28v21n9-13052758tab05.gif" "imagenAlto" => 250 "imagenAncho" => 830 "imagenTamanyo" => 45655 ] ] ] ] ] "descripcion" => array:1 [ "en" => "Patterns of bands obtained in different runs with different DNA extractions. A and B: Two different runs using the first DNA extraction. Figures C and D: Two different runs using the second DNA extraction. Lanes: 1: Size marker; 2-10: A. fumigatus (strain nº 1, 2, 3, 5, 7, 8, 9, 11, 13), 11: A. fumigatus from ATCC." ] ] 5 => array:8 [ "identificador" => "tbl6" "etiqueta" => "Figure 3" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "tabla" => array:1 [ "tablatextoimagen" => array:1 [ 0 => array:1 [ "tablaImagen" => array:1 [ 0 => array:4 [ "imagenFichero" => "28v21n9-13052758tab06.gif" "imagenAlto" => 355 "imagenAncho" => 730 "imagenTamanyo" => 32267 ] ] ] ] ] "descripcion" => array:1 [ "en" => "Patterns of bands obtained in different runs with different DNA extractions. A and B: Two different runs using the first DNA extraction. Figures C and D: Two different runs using the second DNA extraction. Lanes: 1: Size marker; 2-10: A. fumigatus (strain nº 1, 2, 3, 5, 7, 8, 9, 11, 13), 11: A. fumigatus from ATCC." ] ] 6 => array:8 [ "identificador" => "tbl7" "etiqueta" => "Figure 4" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" "tabla" => array:1 [ "tablatextoimagen" => array:1 [ 0 => array:1 [ "tablaImagen" => array:1 [ 0 => array:4 [ "imagenFichero" => "28v21n9-13052758tab07.gif" "imagenAlto" => 656 "imagenAncho" => 730 "imagenTamanyo" => 66057 ] ] ] ] ] "descripcion" => array:1 [ "en" => "DNA from A. fumigatus strains amplified by RAPD-PCR using the OPZ-19 primer. Lanes: 1: PCR marker (bp); 2-10: A. fumigatus (strains number 1, 2, 3, 5, 7, 8, 9, 11, 13); 11: A. fumigatus from ATCC." ] ] 7 => array:5 [ "identificador" => "tbl8" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" ] 8 => array:5 [ "identificador" => "tbl9" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" ] 9 => array:5 [ "identificador" => "tbl10" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" ] 10 => array:5 [ "identificador" => "tbl11" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" ] 11 => array:5 [ "identificador" => "tbl12" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" ] 12 => array:5 [ "identificador" => "tbl13" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" ] 13 => array:5 [ "identificador" => "tbl14" "tipo" => "MULTIMEDIATABLA" "mostrarFloat" => true "mostrarDisplay" => false "copyright" => "Elsevier España" ] ] "bibliografia" => array:2 [ "titulo" => "Bibliography" "seccion" => array:1 [ 0 => array:1 [ "bibliografiaReferencia" => array:16 [ 0 => array:3 [ "identificador" => "bib1" "etiqueta" => "1" "referencia" => array:1 [ 0 => array:3 [ "referenciaCompleta" => "Apergillosis. 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Original language: English
Year/Month | Html | Total | |
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2024 October | 52 | 21 | 73 |
2024 September | 58 | 17 | 75 |
2024 August | 67 | 14 | 81 |
2024 July | 63 | 16 | 79 |
2024 June | 53 | 9 | 62 |
2024 May | 72 | 11 | 83 |
2024 April | 68 | 5 | 73 |
2024 March | 59 | 3 | 62 |
2024 February | 72 | 7 | 79 |
2024 January | 68 | 9 | 77 |
2023 December | 88 | 9 | 97 |
2023 November | 79 | 10 | 89 |
2023 October | 150 | 12 | 162 |
2023 September | 46 | 1 | 47 |
2023 August | 56 | 8 | 64 |
2023 July | 76 | 10 | 86 |
2023 June | 89 | 6 | 95 |
2023 May | 106 | 12 | 118 |
2023 April | 98 | 66 | 164 |
2023 March | 90 | 8 | 98 |
2023 February | 79 | 15 | 94 |
2023 January | 69 | 10 | 79 |
2022 December | 83 | 15 | 98 |
2022 November | 116 | 16 | 132 |
2022 October | 63 | 10 | 73 |
2022 September | 69 | 24 | 93 |
2022 August | 73 | 22 | 95 |
2022 July | 69 | 12 | 81 |
2022 June | 50 | 18 | 68 |
2022 May | 81 | 15 | 96 |
2022 April | 67 | 9 | 76 |
2022 March | 82 | 24 | 106 |
2022 February | 67 | 12 | 79 |
2022 January | 63 | 13 | 76 |
2021 December | 55 | 16 | 71 |
2021 November | 48 | 9 | 57 |
2021 October | 69 | 54 | 123 |
2021 September | 64 | 11 | 75 |
2021 August | 44 | 6 | 50 |
2021 July | 31 | 12 | 43 |
2021 June | 32 | 10 | 42 |
2021 May | 39 | 7 | 46 |
2021 April | 80 | 28 | 108 |
2021 March | 62 | 9 | 71 |
2021 February | 39 | 12 | 51 |
2021 January | 48 | 11 | 59 |
2020 December | 40 | 18 | 58 |
2020 November | 34 | 10 | 44 |
2020 October | 28 | 8 | 36 |
2020 September | 30 | 17 | 47 |
2020 August | 37 | 14 | 51 |
2020 July | 31 | 10 | 41 |
2020 June | 19 | 10 | 29 |
2020 May | 57 | 12 | 69 |
2020 April | 39 | 5 | 44 |
2020 March | 48 | 12 | 60 |
2020 February | 41 | 5 | 46 |
2020 January | 57 | 10 | 67 |
2019 December | 56 | 23 | 79 |
2019 November | 45 | 12 | 57 |
2019 October | 54 | 11 | 65 |
2019 September | 72 | 17 | 89 |
2019 August | 35 | 4 | 39 |
2019 July | 71 | 12 | 83 |
2019 June | 125 | 52 | 177 |
2019 May | 265 | 134 | 399 |
2019 April | 127 | 78 | 205 |
2019 March | 25 | 9 | 34 |
2019 February | 33 | 18 | 51 |
2019 January | 28 | 8 | 36 |
2018 December | 16 | 14 | 30 |
2018 November | 18 | 9 | 27 |
2018 October | 36 | 10 | 46 |
2018 September | 16 | 6 | 22 |
2018 August | 9 | 10 | 19 |
2018 July | 19 | 9 | 28 |
2018 June | 10 | 6 | 16 |
2018 May | 10 | 18 | 28 |
2018 April | 3 | 3 | 6 |
2018 March | 15 | 4 | 19 |
2018 February | 10 | 6 | 16 |
2018 January | 21 | 8 | 29 |
2017 December | 10 | 2 | 12 |
2017 November | 19 | 5 | 24 |
2017 October | 8 | 7 | 15 |
2017 September | 16 | 2 | 18 |
2017 August | 10 | 7 | 17 |
2017 July | 8 | 3 | 11 |
2017 June | 13 | 4 | 17 |
2017 May | 26 | 6 | 32 |
2017 April | 12 | 6 | 18 |
2017 March | 18 | 1 | 19 |
2017 February | 23 | 2 | 25 |
2017 January | 16 | 0 | 16 |
2016 December | 18 | 4 | 22 |
2016 November | 21 | 5 | 26 |
2016 October | 59 | 4 | 63 |
2016 September | 38 | 2 | 40 |
2016 August | 30 | 3 | 33 |
2016 July | 11 | 1 | 12 |
2016 June | 23 | 6 | 29 |
2016 May | 24 | 17 | 41 |
2016 April | 22 | 5 | 27 |
2016 March | 18 | 10 | 28 |
2016 February | 14 | 11 | 25 |
2016 January | 24 | 18 | 42 |
2015 December | 15 | 10 | 25 |
2015 November | 20 | 10 | 30 |
2015 October | 26 | 13 | 39 |
2015 September | 23 | 6 | 29 |
2015 August | 12 | 4 | 16 |
2015 July | 12 | 3 | 15 |
2015 June | 9 | 2 | 11 |
2015 May | 16 | 5 | 21 |
2015 April | 21 | 14 | 35 |
2015 March | 32 | 9 | 41 |
2015 February | 22 | 3 | 25 |
2015 January | 25 | 4 | 29 |
2014 December | 21 | 3 | 24 |
2014 November | 24 | 1 | 25 |
2014 October | 22 | 1 | 23 |
2014 September | 21 | 2 | 23 |
2014 August | 14 | 2 | 16 |
2014 July | 22 | 3 | 25 |
2014 June | 23 | 2 | 25 |
2014 May | 11 | 1 | 12 |
2014 April | 16 | 3 | 19 |
2014 March | 17 | 2 | 19 |
2014 February | 14 | 3 | 17 |
2014 January | 19 | 1 | 20 |
2013 December | 20 | 2 | 22 |
2013 November | 17 | 7 | 24 |
2013 October | 23 | 4 | 27 |
2013 September | 21 | 8 | 29 |
2013 August | 37 | 8 | 45 |
2013 July | 36 | 1 | 37 |
2003 November | 1083 | 0 | 1083 |