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New procedure for rapid identification of microorganisms causing urinary tract infection from urine samples by mass spectrometry (MALDI-TOF)
Nuevo procedimiento para la identificación rápida en muestras de orina de organismos causantes de infecciones del tracto urinario por espectometría de masas (MALDI-TOF)
Gabriel Alberto March Rossellóa,
Corresponding author
gmr810@hotmail.com

Corresponding author.
, María Purificación Gutiérrez Rodrígueza, Raúl Ortiz de Lejarazu Leonardoa,b, Antonio Orduña Domingoa,b, Miguel Ángel Bratos Péreza,b
a Departmento de Microbiología, Facultad de Medicina, Universidad de Valladolid, Spain
b Servicio de Microbiología, Hospital Clínico Universitario de Valladolid, Spain
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        "titulo" => "Nuevo procedimiento para la identificaci&#243;n r&#225;pida en muestras de orina de organismos causantes de infecciones del tracto urinario por espectometr&#237;a de masas &#40;MALDI-TOF&#41;"
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    "textoCompleto" => "<span class="elsevierStyleSections"><span id="sec0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0065">Introduction</span><p id="par0005" class="elsevierStylePara elsevierViewall">Urinary tract infections &#40;UTIs&#41; can manifest in a wide clinical range from bacteriuria with limited clinical symptoms to sepsis&#44; severe sepsis or septic shock<a class="elsevierStyleCrossRef" href="#bib0005"><span class="elsevierStyleSup">1</span></a>&#46; In 20&#8211;30&#37; of all septic patients the infection focus is localized in the urogenital tract<a class="elsevierStyleCrossRef" href="#bib0010"><span class="elsevierStyleSup">2</span></a> and an adequate initial antibiotic therapy is essential since it ensures improved outcome<a class="elsevierStyleCrossRef" href="#bib0015"><span class="elsevierStyleSup">3</span></a>&#46; Moreover&#44; inappropriate antimicrobial therapy in severe UTI is linked to a higher mortality rate<a class="elsevierStyleCrossRef" href="#bib0020"><span class="elsevierStyleSup">4</span></a>&#46; For election of the empiric treatment in these cases&#44; identification directly from urine samples of bacteria causing infection can be useful&#46;</p><p id="par0010" class="elsevierStylePara elsevierViewall">Matrix assisted laser desorption&#47;ionization-time of flight &#40;MALDI-TOF&#41; technology is a technique which allows microbial identification in clinical microbiology laboratories based on microbial protein profiles<a class="elsevierStyleCrossRef" href="#bib0025"><span class="elsevierStyleSup">5</span></a>&#46; A promising application of the MALDI-TOF technique consists of carrying out identification from direct urine samples&#44; without the need to cultivate them&#46; In this way&#44; a result at microbiological species level can be obtained in less than one hour&#46;</p><p id="par0015" class="elsevierStylePara elsevierViewall">Validation criteria of the results indicated by the manufacturers are restrictive&#44; such that only high scoring identification is accepted&#46; On the other hand&#44; when microbial identification is carried out by MALDI-TOF from direct samples&#44; the identification scores obtained are lower than scores obtained from culture&#44; as a result of possible analytical interferences&#44; which were not eliminated&#46; So&#44; in order to accept a higher rate of identification&#44; the required level of validation criterion from the manufacturer should be lowered&#46; In fact&#44; various criteria have been published for validation of results that provided different rates of acceptable direct identifications<a class="elsevierStyleCrossRefs" href="#bib0030"><span class="elsevierStyleSup">6&#8211;9</span></a>&#46;</p><p id="par0020" class="elsevierStylePara elsevierViewall">The objectives of this study were &#40;i&#41; to develop a differential centrifugation protocol which allows the identification of microorganisms by MALDI-TOF from direct urine samples&#44; &#40;ii&#41; to propose a new validating criterion for the results that allows to increase the number of acceptable direct identifications&#44; and &#40;iii&#41; to examine the influence of bacterial concentration in urine on the accuracy of direct identification by MALDI-TOF&#46;</p></span><span id="sec0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0070">Material and methods</span><span id="sec0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0075">Determination of sample size</span><p id="par0025" class="elsevierStylePara elsevierViewall">Assuming that the population was made up of positive urine samples in the period of one year &#40;3272 in 2010 at Microbiology Service at the University Clinic Hospital of Valladolid&#44; Spain&#41; and accepting an alpha risk of 0&#46;95 giving a precision of &#177;0&#46;1 units in bilateral contrast to a proportion estimated at 0&#46;5&#44; for the validation of the differential centrifugation protocol&#44; the study group would need to be made up of 95 monobacterial positive urine samples with counts of &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#46; A hundred samples were randomly processed to facilitate calculation&#46; The samples were obtained through midstream clean catch and only monobacterial samples were selected because more than 95&#37; UTIs are caused by single bacterial species<a class="elsevierStyleCrossRef" href="#bib0050"><span class="elsevierStyleSup">10</span></a>&#46;</p><p id="par0030" class="elsevierStylePara elsevierViewall">In order to study the relationship between bacterial concentration in urine samples and the accuracy of identification by MALDI-TOF from direct samples&#44; 37 urines with counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml were processed &#40;14 samples with counts between 5<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span>and 1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#44; 17 samples with counts between 1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span> and 5<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#44; and six samples with counts &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#41;&#46; Additionally&#44; four samples with yeast were processed&#46; Briefly&#44; a total of 141 monomicrobial positive urine samples were processed&#46;</p></span><span id="sec0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0080">Working procedure</span><p id="par0035" class="elsevierStylePara elsevierViewall">In order to obtain direct identification&#44; the urine samples were processed as follows&#58; samples were homogenized by agitation&#44; and 5<span class="elsevierStyleHsp" style=""></span>ml was centrifuged for 5<span class="elsevierStyleHsp" style=""></span>min at 700<span class="elsevierStyleHsp" style=""></span>rpm&#46; The supernatant obtained was centrifuged for 15<span class="elsevierStyleHsp" style=""></span>min at 6000<span class="elsevierStyleHsp" style=""></span>rpm&#46; The sediment was resuspended in 1<span class="elsevierStyleHsp" style=""></span>ml of HPLC quality distilled water and was centrifuged again for 15<span class="elsevierStyleHsp" style=""></span>min at 6000<span class="elsevierStyleHsp" style=""></span>rpm&#46; The proteins of the sediment were extracted using the ethanol&#47;formic acid technique&#46; In order to carry out this extraction the sample was mixed with 300<span class="elsevierStyleHsp" style=""></span>&#956;l of HPLC quality distilled water and 900<span class="elsevierStyleHsp" style=""></span>&#956;l of absolute ethanol&#44; and vortexed until a homogeneous suspension was achieved&#46; The mixture was centrifuged for 2<span class="elsevierStyleHsp" style=""></span>min at 13&#44;000<span class="elsevierStyleHsp" style=""></span>rpm and the supernatant was eliminated&#46; Finally&#44; 25<span class="elsevierStyleHsp" style=""></span>&#956;l of 70&#37; formic acid and 25<span class="elsevierStyleHsp" style=""></span>&#956;l of acetonitrile were added&#44; vortexed and centrifuged for 1<span class="elsevierStyleHsp" style=""></span>min at 13&#44;000<span class="elsevierStyleHsp" style=""></span>rpm&#46; Using the supernatant obtained&#44; four 1<span class="elsevierStyleHsp" style=""></span>&#956;l spots were dropped on four different areas of the metal sample plate &#40;MSP 96 target polished steel&#59; Bruker&#44; Bremen&#44; Germany&#41;&#46; They were allowed to dry and 1<span class="elsevierStyleHsp" style=""></span>&#956;l of the matrix solution &#40;saturated solution of cyano-4-hydroxy cinnamic acid in 50&#37; acetonitrile with 2&#46;5&#37; trifluoroacetic acid&#41; was added to each spot to be analyzed&#46; They were allowed to dry again and then&#44; a reading of the four spots from each sample was taken&#46; All chemicals were supplied by Sigma&#8211;Aldrich &#40;St Louis&#44; MO&#44; USA&#41;&#46;</p><p id="par0040" class="elsevierStylePara elsevierViewall">Furthermore&#44; at the same time&#44; urine samples were plated&#44; using a calibrated loop so as to determine the number or CFU&#47;ml present in the urine&#44; on Columbia agar plates supplemented with 5&#37; sheep&#39;s blood &#40;bioM&#233;rieux&#44; Marcy l&#8217;Etoile&#44; France&#41; and MacConkey medium &#40;bioM&#233;rieux&#44; Marcy l&#8217;Etoile&#44; France&#41;&#59; in addition a Sabouraud<span class="elsevierStyleHsp" style=""></span>&#43;<span class="elsevierStyleHsp" style=""></span>Chloramphenicol agar plate &#40;Difco&#44; Maryland&#44; USA&#41; was added in those cases where fungal analysis was requested&#46; The plates were incubated at 37<span class="elsevierStyleHsp" style=""></span>&#176;C&#47;24<span class="elsevierStyleHsp" style=""></span>h in aerobic atmosphere and identification was done by MALDI technique&#46; Samples showing growth of two or more microorganisms were not included in the study&#46;</p><p id="par0045" class="elsevierStylePara elsevierViewall">Protein analysis was carried out using a mass spectrometer MALDI Microflex LT &#40;BrukerDaltoniK GmbH&#44; Bremen&#44; Germany&#41; with FLexControl v&#46; 3&#46;0 software &#40;Bruker DaltoniK GmbH&#44; Bremen&#44; Germany&#41;&#46; The spectrum was obtained in linear mode &#40;N<span class="elsevierStyleInf">2</span> laser frequency and wavelength&#58; 60<span class="elsevierStyleHsp" style=""></span>Hz and 337<span class="elsevierStyleHsp" style=""></span>nm&#44; voltage of the ion source I&#58; 20<span class="elsevierStyleHsp" style=""></span>kV&#44; voltage of the ion source II&#58; 16&#46;7<span class="elsevierStyleHsp" style=""></span>kV&#59; detector mass range&#58; 2000&#8211;20&#44;137<span class="elsevierStyleHsp" style=""></span>Da&#41; and 240 shots from different positions of the metal sample plate were recorded&#46; Once the association is completed&#44; the Biotyper v&#46;3&#46;0 software &#40;Bruker DaltoniK GmbH&#44; Bremen&#44; Germany&#41; suggests a list of ten most likely microorganism identifications&#44; each with a score that indicates its accuracy<a class="elsevierStyleCrossRefs" href="#bib0055"><span class="elsevierStyleSup">11&#44;12</span></a>&#46; Adopting the manufacturer&#39;s criteria&#44; valid identifications may be deemed as those in which a score of &#8805;2&#46;000 is obtained for the first microorganism on the list&#46; The software also offered a consistency index&#44; which provides information concerning the quality of the identification that does not affect the validity of the results&#46; This consistency index depends on the concordance of the microbial identification obtained on the ten microorganisms on the list&#46;</p><p id="par0050" class="elsevierStylePara elsevierViewall">Results of the identification were interpreted in accordance with previously published criteria<a class="elsevierStyleCrossRefs" href="#bib0030"><span class="elsevierStyleSup">6&#8211;9</span></a>&#59; in those cases in which the criteria of the different authors were based on a single reading&#44; the first of the four replicates carried out was chosen and the remaining three replicates were rejected&#46; For this&#44; urine samples were divided into two subgroups&#44; one including the study group of 100 monobacterial samples with counts of &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#44; and another including the 37 urine samples with counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#46; Moreover&#44; for each urine sample&#44; MALDI-TOF scores from direct sample and from colonies grown on plates were compared and&#44; taking the identification obtained by MALDI-TOF from culture using the criteria of the manufacturer as the gold standard&#44; a validation criterion for direct identification was defined&#46;</p><p id="par0055" class="elsevierStylePara elsevierViewall">In order to compare the identification results obtained using the different validation criteria&#44; the McNemar test was used&#46; In order to compare the frequency distribution in the population of the study group&#44; the Chi-squared test was used&#46; All these calculations were carried out using the SPSS software v&#46; 15&#46;0&#46;</p></span></span><span id="sec0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0085">Results</span><p id="par0060" class="elsevierStylePara elsevierViewall">No statistically significant difference was observed &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#62;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#41; when comparing isolates obtained from the study group with those from the University Clinic Hospital of Valladolid &#40;Spain&#41; during 2010 &#40;<a class="elsevierStyleCrossRef" href="#tbl0005">Table 1</a>&#41;&#46; Moreover&#44; the four species most frequently isolated in the urine samples from the Hospital were the same as those in the study group&#59; these were&#44; in order of greater to lesser prevalence&#44; <span class="elsevierStyleItalic">Escherichia coli</span> with isolate percentages of 56&#37; in the Hospital and 51&#37; in the study group&#44; <span class="elsevierStyleItalic">Enterococcus faecalis</span> with percentages of 18&#37; and 11&#37; respectively&#44; <span class="elsevierStyleItalic">Klebsiella pneumoniae</span> with percentages of 7&#46;9&#37; and 11&#37; respectively and <span class="elsevierStyleItalic">Proteus mirabilis</span> with percentages of 4&#46;5&#37; and 4&#37; respectively&#46; Of the 100 urine samples that comprised the study group&#44; 63 were from women and 37 from men&#46; The age of the patients was between 4 and 94 years&#44; with an average age of 69 years&#59; and 23 of them were hospitalized&#46;</p><elsevierMultimedia ident="tbl0005"></elsevierMultimedia><p id="par0065" class="elsevierStylePara elsevierViewall">Urine samples with counts of &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml produced higher identification scores &#40;close to or above 2&#46;000&#41; in the majority of cases&#46; On the other hand&#44; the identification scores from urine with counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml were lower&#44; such that identifications with scores &#60;1&#46;400 corresponded with microorganisms which are rarely etiological agents of urinary infection and&#44; in numerous occasions&#44; in one or more determinations of the four microorganisms carried out from the samples no peaks were obtained&#46; Moreover&#44; when scores were &#8805;1&#46;400&#44; despite the fact that the proposed microorganisms were included among the frequent UTIs source&#44; and the identification proposed for each spot was different&#44; the score was not a sufficient criterion for determining the identification of the bacteria&#44; given that&#44; in many cases&#44; the suggestion with a higher score did not coincide with the identification obtained from culture&#46; In this way&#44; we observed that taking as valid the identification that was repeated at least in two of the four spots with counts of &#8805;1&#46;400&#44; we obtained 100&#37; concordance in respect of the identifications carried out from culture&#46;</p><p id="par0070" class="elsevierStylePara elsevierViewall">Based on these data&#44; we proposed the following validation criterion&#58; a microorganism was correctly identified using MALDI-TOF when&#44; after having taken four readings of the same sample&#44; at least two readings offer the same identification proposal at species level for the first microorganism in the list&#44; both with a score of &#8805;1&#46;400&#46;</p><p id="par0075" class="elsevierStylePara elsevierViewall">Identification results obtained from all urine samples processed in accordance with the validation criteria adopted are shown in <a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#46; When the identification results obtained from the study group by applying the criterion proposed in this study were compared with those obtained by applying the validation criteria of different authors<a class="elsevierStyleCrossRefs" href="#bib0030"><span class="elsevierStyleSup">6&#8211;9</span></a>&#44; no statistically significant differences were observed &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#62;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#41;&#46; On the other hand&#44; when the identification results from the 37 urine samples with counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml by applying the criterion proposed in this study were compared&#44; we obtained statistically significant differences with respect to those obtained using the criteria of Moussaoui et al&#46;<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">6</span></a> &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#41;&#44; Schubert et al&#46;<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a> &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#41; and Stevenson et al&#46;<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">8</span></a> &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#60;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#41;&#59; however&#44; no statistically significant differences were observed &#40;<span class="elsevierStyleItalic">p</span><span class="elsevierStyleHsp" style=""></span>&#62;<span class="elsevierStyleHsp" style=""></span>0&#46;05&#41; with respect to the criterion of La Scola and Raoult<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">9</span></a>&#46;</p><elsevierMultimedia ident="tbl0010"></elsevierMultimedia><p id="par0080" class="elsevierStylePara elsevierViewall">Bacterial identification results obtained by MALDI-TOF from 137 urine samples by applying the validation criterion proposed in this study are shown in <a class="elsevierStyleCrossRef" href="#tbl0015">Table 3</a>&#46; Of the 51 samples with counts of &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml in which <span class="elsevierStyleItalic">E&#46; coli</span> was identified from culture&#44; there was only one failed identification from direct urine&#46; The eleven isolates of <span class="elsevierStyleItalic">K&#46; pneumoniae</span>&#44; the four of <span class="elsevierStyleItalic">P&#46; mirabilis</span> and <span class="elsevierStyleItalic">Klebsiella oxytoca</span>&#44; the three of <span class="elsevierStyleItalic">Citrobacter freundii</span>&#44; and the one of <span class="elsevierStyleItalic">Enterobacter aerogenes</span> and <span class="elsevierStyleItalic">Serratia marcescens</span> identified from culture were correctly identified from urine&#46; Finally&#44; of the two samples in which <span class="elsevierStyleItalic">Enterobacter cloacae</span> was isolated&#44; correct identification was obtained in one of them&#46; Of the four samples in which non-fermenting Gram-negative rods &#40;NFGNR&#41; were identified &#40;two urine samples with <span class="elsevierStyleItalic">Pseudomonas aeruginosa</span>&#44; one with <span class="elsevierStyleItalic">Acinetobacter baumannii</span> and one with <span class="elsevierStyleItalic">Acinetobacter genomospecies 3</span>&#41;&#44; there was only one failed identification&#44; which corresponded to a urine with <span class="elsevierStyleItalic">P&#46; aeruginosa</span>&#46; <span class="elsevierStyleItalic">Staphylococci</span> were isolated in four samples &#40;two urine samples with <span class="elsevierStyleItalic">Staphylococcus aureus</span>&#44; one with <span class="elsevierStyleItalic">Staphylococcus epidermidis</span> and one with <span class="elsevierStyleItalic">Staphylococcus saprophyticus</span>&#41;&#59; in this set of four samples&#44; only one correct direct identification was obtained&#44; which corresponded to one isolate of <span class="elsevierStyleItalic">S&#46; aureus</span>&#46; <span class="elsevierStyleItalic">E&#46; faecalis</span> was isolated from eleven samples&#44; and from ten of them correct identification was obtained from urine directly&#46; In the same way&#44; two correct identifications were obtained from a set of five samples with <span class="elsevierStyleItalic">Streptococcus agalactiae</span>&#46; In this way&#44; a 90&#37; sensitivity was obtained &#40;confidence interval of 95&#37;&#58; 81&#46;96&#8211;94&#46;84&#41;&#46;</p><elsevierMultimedia ident="tbl0015"></elsevierMultimedia><p id="par0085" class="elsevierStylePara elsevierViewall">Of the 37 urine samples with counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml direct identification was achieved in seven of them&#46; Of the 14 urine samples with counts between 5<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span> and 1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml there were ten with Gram-negative bacterial isolates&#44; and from these three correct identifications were obtained&#44; which were two isolates of <span class="elsevierStyleItalic">E&#46; coli</span> and one of <span class="elsevierStyleItalic">K&#46; pneumoniae</span>&#46; Three isolates of <span class="elsevierStyleItalic">E&#46; coli</span>&#44; one of <span class="elsevierStyleItalic">P&#46; mirabilis</span> and one of <span class="elsevierStyleItalic">Morganella morganii</span> were not identified from the sample directly&#46; On the other hand&#44; of the four samples with Gram-positive bacterial isolates &#40;two urines with an isolate of <span class="elsevierStyleItalic">E&#46; faecalis</span>&#44; one with <span class="elsevierStyleItalic">Enterococcus faecium</span> and one with <span class="elsevierStyleItalic">S&#46; agalactiae</span>&#41; only one identification of <span class="elsevierStyleItalic">E&#46; faecalis</span> was obtained&#46;</p><p id="par0090" class="elsevierStylePara elsevierViewall">Of the 17 urine samples with counts between 1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span> and 5<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml there were 11 with Gram-negative bacterial isolates which provided three acceptable identifications of <span class="elsevierStyleItalic">E&#46; coli</span> from a direct sample&#59; three isolates of <span class="elsevierStyleItalic">E&#46; coli</span>&#44; two of <span class="elsevierStyleItalic">P&#46; mirabilis</span> and three of <span class="elsevierStyleItalic">P&#46; aeruginosa</span> were not identified&#46; In the six samples with Gram-positive bacterial isolates no correct identification was obtained&#46; In this case&#44; the strains identified from culture were one of <span class="elsevierStyleItalic">S&#46; aureus</span>&#44; four of <span class="elsevierStyleItalic">E&#46; faecalis</span> and one of <span class="elsevierStyleItalic">S&#46; agalactiae</span>&#46;</p><p id="par0095" class="elsevierStylePara elsevierViewall">Of the six urine samples processed with counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#44; no valid identification was obtained&#46; Five strains of <span class="elsevierStyleItalic">E&#46; coli</span> and one of <span class="elsevierStyleItalic">P&#46; mirabilis</span> were identified from culture&#46;</p><p id="par0100" class="elsevierStylePara elsevierViewall">As can be seen in <a class="elsevierStyleCrossRef" href="#tbl0015">Table 3</a>&#44; as the bacterial concentration was reduced in the samples&#44; the percentages of identification and average scores obtained were also reduced&#46; For each urinary bacterial concentration&#44; the bacteria belonging to the family Enterobacteriaceae showed the greater percentage of correct identifications and higher average score&#44; and in the same way&#44; the percentage of correct identifications and the average score in Gram-negative bacteria were greater than that of Gram-positive bacteria&#46;</p><p id="par0105" class="elsevierStylePara elsevierViewall">In the identification of yeasts by MALDI-TOF from four urine samples&#44; the species identified from culture was <span class="elsevierStyleItalic">Candida albicans</span>&#46; In the two urine samples with counts of &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml identification was obtained with an average score of 1&#46;989&#44; and in the two with counts between 1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span> and 5<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml no valid identification was obtained&#46;</p></span><span id="sec0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0090">Discussion</span><p id="par0110" class="elsevierStylePara elsevierViewall">This paper was focused on the direct microbial identification in urine samples regardless of their concentration because&#44; apart from the classic criterion described by Kass<a class="elsevierStyleCrossRef" href="#bib0065"><span class="elsevierStyleSup">13</span></a>&#44; it had demonstrated that counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml of bacteria and yeasts might be indicative of UTI in samples obtained through midstream clean catch<a class="elsevierStyleCrossRefs" href="#bib0070"><span class="elsevierStyleSup">14&#8211;18</span></a>&#46;</p><p id="par0115" class="elsevierStylePara elsevierViewall">The criteria for validation of results obtained using MALDI-TOF can be classified into two groups&#58; one takes into account the score and consistency index obtained in each reading and the other is based only on the score of the first microorganism in the list&#46; Belonging to the first group two proposals have been reported&#46; So&#44; Moussaoui et al&#46;<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">6</span></a> established that an identification is valid if a score of &#8805;1&#46;400 is obtained for the first microorganism in the list and the species in the first four identification proposals coincides&#59; similarly&#44; Schubert et al&#46;<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a> advocated that an identification is valid if a score of &#8805;1&#46;500 is obtained for the first microorganism and the species is the same in the first three identification proposals in the list&#46; Based on the score obtained for the first microorganism in the list given by the software&#44; two criteria can be found in the literature&#44; i&#46;e&#46;&#44; the one proposed by Stevenson et al&#46;<a class="elsevierStyleCrossRef" href="#bib0040"><span class="elsevierStyleSup">8</span></a>&#44; and subsequently by Prod&#8217;hom et al&#46;<a class="elsevierStyleCrossRef" href="#bib0095"><span class="elsevierStyleSup">19</span></a>&#44; Juiz et al&#46;<a class="elsevierStyleCrossRef" href="#bib0100"><span class="elsevierStyleSup">20</span></a> and Ferreira et al&#46;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a>&#44; establishing that a score of &#8805;2&#46;000 in an identification indicates a species level identification&#44; a score between 1&#46;700 and 1&#46;900 indicates a genus level identification&#44; and a score of &#60;1&#46;700 indicates non-identification&#46; Finally&#44; the standard proposed by La Scola and Raoult<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">9</span></a> established that a microorganism is identified correctly using MALDI-TOF when&#44; after having performed four identifications of a single sample&#44; the bacterial species with the highest score &#40;the first on the list&#41; coincides in all of them and a score of &#8805;1&#46;900 is obtained in at least two identifications&#44; or when a score of &#8805;1&#46;200 is obtained in the four identifications&#46;</p><p id="par0120" class="elsevierStylePara elsevierViewall">Urine samples with counts of &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml of Gram-negative bacteria and <span class="elsevierStyleItalic">Enterococcus</span> spp&#46; provided high identification scores &#40;close to 2&#46;000&#41; and therefore high correct identification percentages were obtained&#44; regardless of the validation criteria used&#46; It is worth mentioning that the proposed criterion produced more direct identification percentages in the study group &#40;<a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#41;&#46; However&#44; for the urine samples with counts of &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml of <span class="elsevierStyleItalic">Staphylococcus</span> spp&#46; or <span class="elsevierStyleItalic">Streptococcus</span> spp&#46; and urine with counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#44; low identification scores &#40;close to 1&#46;400&#41; were obtained&#44; and as a consequence&#44; low identification percentages were obtained&#46; For these urine samples with low scores&#44; the identification percentage obtained depended on the criterion used in validating results and our criterion also produced higher percentages of correct direct identification &#40;<a class="elsevierStyleCrossRef" href="#tbl0010">Table 2</a>&#41;&#46; The criteria of Moussaoui et al&#46;<a class="elsevierStyleCrossRef" href="#bib0030"><span class="elsevierStyleSup">6</span></a> and Schubert et al&#46;<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a>&#44; which take into account the score and the consistency index&#44; were not suitable for the validation of these urine samples that provided low identification scores&#46; It was due to the lack of concordance at species level on the list of the ten microorganisms proposed by the software&#44; despite the fact that the first option in the list could coincide with the identification obtained from culture&#46; La Scola and Raoult<a class="elsevierStyleCrossRef" href="#bib0045"><span class="elsevierStyleSup">9</span></a>&#44; who used lower scores than all those published to date&#44; proposed substituting the consistency index obtained in a reading with the concordance at species level obtained in the first identification of the four readings carried out from the same sample&#59; which is to say&#44; this criterion is based on the score and the reproducibility of the equipment&#46; A limitation of this criterion consists in that proposing valid identification from scores of &#8805;1&#46;200 is not useful for validating of the results obtained from direct urine samples with low bacterial counts&#44; since we have observed that scores of &#60;1&#46;400 suggested microorganisms which are rarely etiological agents in UTI&#46; Using the criterion proposed in this study&#44; the higher identification score obtained in one of the four readings carried out from one sample was not a determining factor&#44; given that if an identification with a higher score than any other of those repeated was obtained&#44; this should be discarded&#46; So&#44; with the proposed criterion&#44; the weighting of the score was reduced in the validation of results&#46;</p><p id="par0125" class="elsevierStylePara elsevierViewall">When 5<span class="elsevierStyleHsp" style=""></span>ml of urine with counts of &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml of <span class="elsevierStyleItalic">Staphylococcus</span> spp&#46; and <span class="elsevierStyleItalic">Streptococcus</span> spp&#46; or with counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml was processed&#44; the bacterial concentration was insufficient for carrying out direct identification by MALDI-TOF from urine&#46; This suggested that in order to achieve the direct identification successfully it would be better to process greater sample volumes in order to process a greater amount of bacteria&#46;</p><p id="par0130" class="elsevierStylePara elsevierViewall">No previous studies on direct identifications of yeasts from urine samples have been found in the literature&#46; Although the number of samples studied in which a yeast was isolated is very low&#44; the results obtained coincided with those from bacteria positive urine with regard to the microbial concentration in the sample&#59; which is to say&#44; the two strains of <span class="elsevierStyleItalic">C&#46; albicans</span> present in two urine samples with counts of &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml were correctly identified by applying our criterion and the two strains isolated in two samples with counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml were not&#46;</p><p id="par0135" class="elsevierStylePara elsevierViewall">Results obtained in this study concur with those of Ferreira et al&#46;<a class="elsevierStyleCrossRef" href="#bib0105"><span class="elsevierStyleSup">21</span></a> and with those of Kohling et al&#46;<a class="elsevierStyleCrossRef" href="#bib0110"><span class="elsevierStyleSup">22</span></a>&#44; which also observed that counts of &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml produced higher percentage of direct identification in urine samples&#44; and that concentrations &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml produced low percentages of direct identification&#46; Our results also matched with those published by Christner et al&#46;<a class="elsevierStyleCrossRef" href="#bib0115"><span class="elsevierStyleSup">23</span></a>&#44; who pointed out that with greater bacterial concentration in the sample&#44; greater is the quantity of microorganisms deposited on the metal sample plate and consequently a higher score was obtained&#46; In this way&#44; when the bacterial concentration in the urine sample is high &#40;&#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#41; satisfactory identification percentages are obtained&#44; above all in Gram-negative bacteria&#46; In urine with counts between 5<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span> and 1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml identification percentages were considerably reduced&#59; in urine with counts between 1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span> and 5<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml the percentages were reduced even further&#59; and in urine with counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">4</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#44; no direct identification was achieved&#46; Moreover&#44; for each urine bacterial concentration the average score for Gram-negative bacteria obtained is superior to that of Gram-positive bacteria &#40;<a class="elsevierStyleCrossRef" href="#tbl0015">Table 3</a>&#41;&#59; these data coincides with that published by Schubert et al&#46;<a class="elsevierStyleCrossRef" href="#bib0035"><span class="elsevierStyleSup">7</span></a> who asserted that the MALDI-TOF system offers greater identification capacity for Gram-negative than Gram-positive bacteria&#46;</p><p id="par0140" class="elsevierStylePara elsevierViewall">For performing bacterial identification directly from urine samples&#44; the clinician must request it in patients with suspected severe UTI&#46; Direct bacterial identification cannot be applied in all urine samples because they are those most commonly processed in clinical microbiology laboratories<a class="elsevierStyleCrossRef" href="#bib0120"><span class="elsevierStyleSup">24</span></a>&#46;</p><p id="par0145" class="elsevierStylePara elsevierViewall">Direct identification is reported in less than one hour from the arrival of the urine to the laboratory and only includes the bacterial species present in urine&#46; For confirmation of direct identification and clinical significance&#44; it is necessary to perform bacterial identification and calculate bacterial counts from colonies obtained on isolation plates&#46; Therefore&#44; the clinician must assess clinical significance of direct bacterial identification and adopt appropriate measures regarding antimicrobial therapy&#46;</p><p id="par0150" class="elsevierStylePara elsevierViewall">A problem of direct identification by MALDI-TOF is that polymicrobial samples could provide wrong identifications since the spectra obtained include peaks of two or more microorganisms&#46; This problem has been partially solved with the use of software &#40;Bruker DaltoniK GmbH&#44; Bremen&#44; Germany&#41; which&#44; in some cases&#44; is able to report two bacterial identifications if both microorganisms are in correct proportions&#46;</p><p id="par0155" class="elsevierStylePara elsevierViewall">To sum up&#44; applying the differential centrifugation protocol together with the validation criterion proposed in this study&#44; it was possible to achieve identification by MALDI-TOF of the microorganisms that most frequently cause UTI from direct monomicrobial urine samples with bacterial counts of &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#59; and&#44; it was also possible to improve correct identifications in urine samples with bacterial counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#46;</p></span><span id="sec0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0095">Conflict of interest</span><p id="par0160" class="elsevierStylePara elsevierViewall">The authors declare no conflict of interest&#46;</p></span></span>"
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        "resumen" => "<span id="abst0005" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0010">Introduction</span><p id="spar0005" class="elsevierStyleSimplePara elsevierViewall">Matrix assisted laser desorption&#47;ionization-time of flight &#40;MALDI-TOF&#41; mass spectrometry is widely established as a technique in clinical microbiology laboratories for the identification of microorganisms&#46; Using this technique&#44; it is also possible to obtain the identification of microorganisms from untreated urine samples&#46;</p></span> <span id="abst0010" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0015">Methods</span><p id="spar0010" class="elsevierStyleSimplePara elsevierViewall">In this study&#44; a differential centrifugation protocol and a criterion for validation of the results in order to achieve microbial identification from untreated urine samples are proposed&#46; Additionally&#44; the sensitivity of the analytical procedure in monobacterial urine samples has been evaluated&#46;</p></span> <span id="abst0015" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0020">Results</span><p id="spar0015" class="elsevierStyleSimplePara elsevierViewall">A 90&#37; sensitivity &#40;confidence interval of 81&#46;96&#37;&#8211;94&#46;84&#37;&#41; was obtained in urine samples with bacterial counts of &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#44; and it was possible to improve the percentages of direct identifications from urine samples with bacterial counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#46;</p></span> <span id="abst0020" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0025">Conclusion</span><p id="spar0020" class="elsevierStyleSimplePara elsevierViewall">It is concluded that the MALDI-TOF system is both fast and reliable in the identification of individual microorganisms from untreated urine samples with counts of &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml&#46;</p></span>"
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        "resumen" => "<span id="abst0025" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0035">Introducci&#243;n</span><p id="spar0025" class="elsevierStyleSimplePara elsevierViewall">El uso de la espectrometr&#237;a de masas Matrix Assisted Laser Desorption&#47;Ionization-Time of Flight &#40;MALDI-TOF&#41; es una t&#233;cnica implantada en los laboratorios de Microbiolog&#237;a Cl&#237;nica que permite llevar a cabo la identificaci&#243;n de microorganismos&#46; Una de las aplicaciones de esta t&#233;cnica es la identificaci&#243;n a partir de muestra directa de orina&#46;</p></span> <span id="abst0030" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0040">M&#233;todos</span><p id="spar0030" class="elsevierStyleSimplePara elsevierViewall">En este estudio se propone un protocolo de centrifugaci&#243;n diferencial y un criterio de validaci&#243;n de los resultados para alcanzar la identificaci&#243;n microbiana a partir de muestra directa de orina&#46; Adicionalmente se estudia la sensibilidad del procedimiento anal&#237;tico en muestras de orina monomicrobianas&#46;</p></span> <span id="abst0035" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0045">Resultados</span><p id="spar0035" class="elsevierStyleSimplePara elsevierViewall">Las orinas con recuentos &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>UFC&#47;ml mostraron un 90&#37; de sensibilidad &#40;Intervalo de Confianza de 81&#46;96&#37;&#8211;94&#46;84&#37;&#41; y se logr&#243; mejorar los porcentajes de identificaci&#243;n directa a partir de orinas con recuentas bacterianos &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>UFC&#47;ml&#46;</p></span> <span id="abst0040" class="elsevierStyleSection elsevierViewall"><span class="elsevierStyleSectionTitle" id="sect0050">Conclusiones</span><p id="spar0040" class="elsevierStyleSimplePara elsevierViewall">La espectrometr&#237;a de masas MALDI-TOF es un sistema r&#225;pido y fiable para la identificaci&#243;n de microorganismos a partir de orinas monomicrobianas con recuentos &#8805;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>UFC&#47;ml&#46;</p></span>"
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                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="" valign="top" scope="col" style="border-bottom: 2px solid black">&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Positive urines year 2010 &#40;<span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>3272&#41; &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Study group &#40;<span class="elsevierStyleItalic">N</span><span class="elsevierStyleHsp" style=""></span>&#61;<span class="elsevierStyleHsp" style=""></span>100&#41; &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">Enterobacteriaceae&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">68&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">76&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">NFGNR<a class="elsevierStyleCrossRef" href="#tblfn0005"><span class="elsevierStyleSup">a</span></a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">7&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleItalic">Staphylococcus</span> spp&#46;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">6&#46;5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleItalic">Streptococcus</span> spp&#46;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&#46;9&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">5&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleItalic">Enterococcus</span> spp&#46;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">13&#46;2&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">11&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">Other microorganisms&nbsp;\t\t\t\t\t\t\n
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          "en" => "<p id="spar0045" class="elsevierStyleSimplePara elsevierViewall">Results of identification obtained by MALDI-TOF from culture of positive urine samples from 2010 and from culture in the study group of 100 urine samples&#46;</p>"
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                  \t\t\t\t" class=""><thead title="thead"><tr title="table-row"><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Author &#40;reference&#41;&#44; kind of criterion&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Correct identifications in 100 urines that form the study group &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Correct identifications in 37 urines with counts of &#60;1<span class="elsevierStyleHsp" style=""></span>&#215;<span class="elsevierStyleHsp" style=""></span>10<span class="elsevierStyleSup">5</span><span class="elsevierStyleHsp" style=""></span>CFU&#47;ml <span class="elsevierStyleItalic">n</span> &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th></tr></thead><tbody title="tbody"><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">Moussaoui et al&#46;<span class="elsevierStyleSup">11</span>&#44; score and consistency index&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">84&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;2&#46;7&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">Schubert et al&#46;<span class="elsevierStyleSup">12</span>&#44; score and consistency index&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">84&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;2&#46;7&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">Stevenson et al&#46;<span class="elsevierStyleSup">13</span>&#44; score&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">84&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1 &#40;2&#46;7&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">La Scola and Raoult<span class="elsevierStyleSup">14</span>&#44; score&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">86&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2 &#40;5&#46;4&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">Criterion proposed&#44; score&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">90&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">7 &#40;18&#46;9&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
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                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Mean scores&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Mean scores&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Correct identifications <span class="elsevierStyleItalic">n</span>&#47;<span class="elsevierStyleItalic">N</span> &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Mean scores&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Correct identifications <span class="elsevierStyleItalic">n</span>&#47;<span class="elsevierStyleItalic">N</span> &#40;&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</th><th class="td" title="table-head  " align="left" valign="top" scope="col" style="border-bottom: 2px solid black">Mean scores&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">77&#47;80 &#40;96&#46;3&#41;&nbsp;\t\t\t\t\t\t\n
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                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&#47;10 &#40;30&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;650&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&#47;11 &#40;27&#46;3&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;456&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#47;6 &#40;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>Enterobacteriaceae&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">74&#47;76 &#40;97&#46;4&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&#46;070&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&#47;8 &#40;37&#46;5&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;650&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&#47;8 &#40;37&#46;5&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;456&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span>NFGNR<a class="elsevierStyleCrossRef" href="#tblfn0010"><span class="elsevierStyleSup">a</span></a>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&#47;4 &#40;75&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;809&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#47;2 &#40;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#47;3 &#40;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">Gram-positive bacteria&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">13&#47;20 &#40;65&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;885&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#47;4 &#40;25&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;608&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#47;6 &#40;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Staphylococcus</span> spp&#46;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#47;4 &#40;25&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;528&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#47;1 &#40;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">S&#46; agalactiae</span>&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&#47;5 &#40;40&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;536&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#47;1 &#40;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#47;4 &#40;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top"><span class="elsevierStyleHsp" style=""></span><span class="elsevierStyleItalic">Enterococcus</span> spp&#46;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">10&#47;11 &#40;90&#46;9&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;985&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#47;3 &#40;33&#46;3&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;608&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#47;1 &#40;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr><tr title="table-row"><td class="td" title="table-entry  " align="left" valign="top">Total&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">90&#47;100 &#40;90&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">2&#46;037&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">4&#47;14 &#40;28&#46;6&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;640&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">3&#47;17 &#40;17&#46;6&#37;&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">1&#46;456&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="char" valign="top">0&#47;6 &#40;0&#41;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td><td class="td" title="table-entry  " align="left" valign="top">&#8211;&nbsp;\t\t\t\t\t\t\n
                  \t\t\t\t</td></tr></tbody></table>
                  """
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          "en" => "<p id="spar0055" class="elsevierStyleSimplePara elsevierViewall">Frequency of microorganisms identified by MALDI-TOF from direct urine samples related with bacterial concentration applying the criterion proposed in this study&#46;</p>"
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      "titulo" => "References"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Therapeutic challenges of urosepsis"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "F&#46;M&#46; Wagenlehner"
                            1 => "A&#46; Pilatz"
                            2 => "K&#46;G&#46; Naber"
                            3 => "W&#46; Weidner"
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                    0 => array:2 [
                      "doi" => "10.1111/j.1365-2362.2008.02008.x"
                      "Revista" => array:7 [
                        "tituloSerie" => "Eur J Clin Invest"
                        "fecha" => "2008"
                        "volumen" => "38"
                        "numero" => "Suppl&#46; 2"
                        "paginaInicial" => "45"
                        "paginaFinal" => "49"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18826481"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
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              "identificador" => "bib0010"
              "etiqueta" => "2"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "The epidemiology of the systemic inflammatory response"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "C&#46; Brun-Buisson"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:7 [
                        "tituloSerie" => "Intensive Care Med"
                        "fecha" => "2000"
                        "volumen" => "26"
                        "numero" => "Suppl&#46; 1"
                        "paginaInicial" => "S64"
                        "paginaFinal" => "S74"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10786961"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
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                ]
              ]
            ]
            2 => array:3 [
              "identificador" => "bib0015"
              "etiqueta" => "3"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Surviving sepsis campaign&#58; international guidelines for management of severe sepsis and septic shock&#58; 2008"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "R&#46;P&#46; Dellinger"
                            1 => "M&#46;M&#46; Levy"
                            2 => "J&#46;M&#46; Carlet"
                            3 => "J&#46; Bion"
                            4 => "M&#46;M&#46; Parker"
                            5 => "R&#46; Jaeschke"
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                    0 => array:2 [
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                        "tituloSerie" => "Intensive Care Med"
                        "fecha" => "2008"
                        "volumen" => "34"
                        "paginaInicial" => "17"
                        "paginaFinal" => "60"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18058085"
                            "web" => "Medline"
                          ]
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              ]
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              "identificador" => "bib0020"
              "etiqueta" => "4"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Empiric antibiotic treatment and the misuse of culture results and antibiotic sensitivities in patients with community-acquired bacteraemia due to urinary tract infection"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "G&#46; Elhanan"
                            1 => "M&#46; Sarhat"
                            2 => "R&#46; Raz"
                          ]
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                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:7 [
                        "tituloSerie" => "J Infect"
                        "fecha" => "1997"
                        "volumen" => "35"
                        "paginaInicial" => "283"
                        "paginaFinal" => "288"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/9459403"
                            "web" => "Medline"
                          ]
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            4 => array:3 [
              "identificador" => "bib0025"
              "etiqueta" => "5"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Classification and identification of bacteria by mass spectrometry and computational analysis"
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                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "S&#46; Sauer"
                            1 => "A&#46; Freiwald"
                            2 => "T&#46; Maier"
                            3 => "M&#46; Kube"
                            4 => "R&#46; Reinhardt"
                            5 => "M&#46; Kostrzewa"
                          ]
                        ]
                      ]
                    ]
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                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1371/journal.pone.0002843"
                      "Revista" => array:5 [
                        "tituloSerie" => "PLoS ONE"
                        "fecha" => "2008"
                        "volumen" => "3"
                        "paginaInicial" => "e2843"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/18665227"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                  ]
                ]
              ]
            ]
            5 => array:3 [
              "identificador" => "bib0030"
              "etiqueta" => "6"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Matrix-assisted laser desorption ionization time-of-flight mass spectrometry identifies 90&#37; of bacteria directly from blood culture vials"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "W&#46; Moussaoui"
                            1 => "B&#46; Jaulhac"
                            2 => "A&#46;M&#46; Hoffmann"
                            3 => "B&#46; Ludes"
                            4 => "M&#46; Kostrzewa"
                            5 => "P&#46; Riegel"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1111/j.1469-0691.2010.03356.x"
                      "Revista" => array:6 [
                        "tituloSerie" => "Clin Microbiol Infect"
                        "fecha" => "2010"
                        "volumen" => "16"
                        "paginaInicial" => "1631"
                        "paginaFinal" => "1638"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20825442"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                  ]
                ]
              ]
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            6 => array:3 [
              "identificador" => "bib0035"
              "etiqueta" => "7"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Novel&#44; improved sample preparation for rapid&#44; direct identification from positive blood cultures using matrix-assisted laser desorption&#47;ionization time-of-flight &#40;MALDI-TOF&#41; mass spectrometry"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "S&#46; Schubert"
                            1 => "K&#46; Weinert"
                            2 => "C&#46; Wagner"
                            3 => "B&#46; Gunzl"
                            4 => "A&#46; Wieser"
                            5 => "T&#46; Maier"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.jmoldx.2011.07.004"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Mol Diagn"
                        "fecha" => "2011"
                        "volumen" => "13"
                        "paginaInicial" => "701"
                        "paginaFinal" => "706"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/21889611"
                            "web" => "Medline"
                          ]
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                ]
              ]
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            7 => array:3 [
              "identificador" => "bib0040"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:3 [
                            0 => "L&#46;G&#46; Stevenson"
                            1 => "S&#46;K&#46; Drake"
                            2 => "P&#46;R&#46; Murray"
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                      ]
                    ]
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                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1128/JCM.01541-09"
                      "Revista" => array:6 [
                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "2010"
                        "volumen" => "48"
                        "paginaInicial" => "444"
                        "paginaFinal" => "447"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19955282"
                            "web" => "Medline"
                          ]
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                ]
              ]
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            8 => array:3 [
              "identificador" => "bib0045"
              "etiqueta" => "9"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Direct identification of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "B&#46; La Scola"
                            1 => "D&#46; Raoult"
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                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1371/journal.pone.0008041"
                      "Revista" => array:5 [
                        "tituloSerie" => "PLoS ONE"
                        "fecha" => "2009"
                        "volumen" => "4"
                        "paginaInicial" => "e8041"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/19946369"
                            "web" => "Medline"
                          ]
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                ]
              ]
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            9 => array:3 [
              "identificador" => "bib0050"
              "etiqueta" => "10"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Urinary tract infections"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:2 [
                            0 => "J&#46;D&#46; Sobel"
                            1 => "D&#46; Kaye"
                          ]
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                    0 => array:1 [
                      "LibroEditado" => array:5 [
                        "titulo" => "Mandell&#44; Douglas&#44; and Bennett&#39;s principles and practice of infectious disease"
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                        "serieFecha" => "2005"
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            10 => array:3 [
              "identificador" => "bib0055"
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              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Microorganism identification by mass spectrometry and protein database searches"
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                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "P&#46;A&#46; Demirev"
                            1 => "Y&#46;P&#46; Ho"
                            2 => "V&#46; Ryzhov"
                            3 => "C&#46; Fenselau"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Anal Chem"
                        "fecha" => "1999"
                        "volumen" => "71"
                        "paginaInicial" => "2732"
                        "paginaFinal" => "2738"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/10424165"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                  ]
                ]
              ]
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            11 => array:3 [
              "identificador" => "bib0060"
              "etiqueta" => "12"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Compilation of a MALDI-TOF mass spectral database for the rapid screening and characterisation of bacteria implicated in human infectious diseases"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
                            0 => "C&#46;J&#46; Keys"
                            1 => "D&#46;J&#46; Dare"
                            2 => "H&#46; Sutton"
                            3 => "G&#46; Wells"
                            4 => "M&#46; Lunt"
                            5 => "T&#46; McKenna"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1016/j.meegid.2004.02.004"
                      "Revista" => array:6 [
                        "tituloSerie" => "Infect Genet Evol"
                        "fecha" => "2004"
                        "volumen" => "4"
                        "paginaInicial" => "221"
                        "paginaFinal" => "242"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15450202"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            12 => array:3 [
              "identificador" => "bib0065"
              "etiqueta" => "13"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Asymptomatic infections of the urinary tract"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:1 [
                            0 => "E&#46;H&#46; Kass"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Trans Assoc Am Physicians"
                        "fecha" => "1956"
                        "volumen" => "69"
                        "paginaInicial" => "56"
                        "paginaFinal" => "64"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/13380946"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            13 => array:3 [
              "identificador" => "bib0070"
              "etiqueta" => "14"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Clinical significance of bacteriuria with low colony counts of <span class="elsevierStyleItalic">Enterococcus</span> species"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "R&#46; Colodner"
                            1 => "T&#46; Eliasberg"
                            2 => "B&#46; Chazan"
                            3 => "R&#46; Raz"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1007/s10096-006-0132-0"
                      "Revista" => array:6 [
                        "tituloSerie" => "Eur J Clin Microbiol Infect Dis"
                        "fecha" => "2006"
                        "volumen" => "25"
                        "paginaInicial" => "238"
                        "paginaFinal" => "241"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/16596356"
                            "web" => "Medline"
                          ]
                        ]
                      ]
                    ]
                  ]
                ]
              ]
            ]
            14 => array:3 [
              "identificador" => "bib0075"
              "etiqueta" => "15"
              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Urinary tract infections associated with <span class="elsevierStyleItalic">Candida albicans</span>"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:6 [
                            0 => "P&#46; Behzadi"
                            1 => "E&#46; Behzadi"
                            2 => "H&#46; Yazdanbod"
                            3 => "R&#46; Aghapour"
                            4 => "M&#46; Akbari Cheshmeh"
                            5 => "D&#46; Salehian Omran"
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                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:5 [
                        "tituloSerie" => "Maedica &#40;Buchar&#41;"
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                        "paginaInicial" => "277"
                        "paginaFinal" => "279"
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                    ]
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              ]
            ]
            15 => array:3 [
              "identificador" => "bib0080"
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              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Urinary tract infection in men"
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                            0 => "E&#46; Hummers-Pradier"
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                            2 => "M&#46; Koch"
                            3 => "W&#46;R&#46; Heizmann"
                            4 => "M&#46;M&#46; Kochen"
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                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:7 [
                        "tituloSerie" => "Int J Clin Pharmacol Ther"
                        "fecha" => "2004"
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                        "paginaInicial" => "360"
                        "paginaFinal" => "366"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/15605687"
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              "identificador" => "bib0085"
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                    0 => array:2 [
                      "titulo" => "Urinary tract infections with low and high colony counts in young women&#46; Spontaneous remission and single-dose vs multiple-day treatment"
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                        0 => array:2 [
                          "etal" => false
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                      ]
                    ]
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                  "host" => array:1 [
                    0 => array:1 [
                      "Revista" => array:6 [
                        "tituloSerie" => "Arch Intern Med"
                        "fecha" => "1994"
                        "volumen" => "154"
                        "paginaInicial" => "300"
                        "paginaFinal" => "304"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/8297196"
                            "web" => "Medline"
                          ]
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                      ]
                    ]
                  ]
                ]
              ]
            ]
            17 => array:3 [
              "identificador" => "bib0090"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Fungal urinary tract infection in burn patients with long-term Foley catheterization"
                      "autores" => array:1 [
                        0 => array:2 [
                          "etal" => false
                          "autores" => array:4 [
                            0 => "J&#46; Kim"
                            1 => "D&#46;S&#46; Kim"
                            2 => "Y&#46;S&#46; Lee"
                            3 => "N&#46;G&#46; Choi"
                          ]
                        ]
                      ]
                    ]
                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.4111/kju.2011.52.9.626"
                      "Revista" => array:6 [
                        "tituloSerie" => "Korean J Urol"
                        "fecha" => "2011"
                        "volumen" => "52"
                        "paginaInicial" => "626"
                        "paginaFinal" => "631"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/22025959"
                            "web" => "Medline"
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                    ]
                  ]
                ]
              ]
            ]
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              "identificador" => "bib0095"
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              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Matrix-assisted laser desorption ionization-time of flight mass spectrometry for direct bacterial identification from positive blood culture pellets"
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                        "tituloSerie" => "J Clin Microbiol"
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                        "paginaInicial" => "1481"
                        "paginaFinal" => "1483"
                        "link" => array:1 [
                          0 => array:2 [
                            "url" => "https://www.ncbi.nlm.nih.gov/pubmed/20164269"
                            "web" => "Medline"
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                ]
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              "identificador" => "bib0100"
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                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "A comparative study of two different methods of sample preparation for positive blood cultures for the rapid identification of bacteria using MALDI-TOF MS"
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                        0 => array:2 [
                          "etal" => true
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                        "paginaFinal" => "1358"
                        "link" => array:1 [
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                          ]
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                ]
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            20 => array:3 [
              "identificador" => "bib0105"
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              "referencia" => array:1 [
                0 => array:2 [
                  "contribucion" => array:1 [
                    0 => array:2 [
                      "titulo" => "Direct identification of urinary tract pathogens from urine samples by matrix-assisted laser desorption ionization-time of flight mass spectrometry"
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                        0 => array:2 [
                          "etal" => true
                          "autores" => array:6 [
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                            1 => "F&#46; Sanchez-Juanes"
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                            5 => "J&#46;M&#46; Gonzalez-Buitrago"
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                  ]
                  "host" => array:1 [
                    0 => array:2 [
                      "doi" => "10.1128/JCM.02215-09"
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                        "tituloSerie" => "J Clin Microbiol"
                        "fecha" => "2010"
                        "volumen" => "48"
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es en pt

¿Es usted profesional sanitario apto para prescribir o dispensar medicamentos?

Are you a health professional able to prescribe or dispense drugs?

Você é um profissional de saúde habilitado a prescrever ou dispensar medicamentos