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Vol. 22. Issue 7.
Pages 390-391 (August 2004)
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Vol. 22. Issue 7.
Pages 390-391 (August 2004)
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Assessment of three rapid methods for the detection of methicillin-resistant Staphylococcus aureus
Evaluación de tres métodos rápidos para la detección de Staphylococcus aureus resistente a la meticilina
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Maria João Soares1,a, Carlos Soaresa, Ana Constança Mendesb, Maria Luís Guimarãesb, José Manuel Cabedab, José Manuel Amorimaa
a Microbiology Service
b Molecular Biology Unit. Hospital Geral de Santo António. Porto. Portugal
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We evaluated three rapid methods to detect methicillin-resistant Staphylococcus aureus (MRSA) and compared them with PCR amplification of mecA. A total of 103 S. aureus strains were studied by MRSA-Screen, BBL Crystal, Velogene Genomic and mecA PCR. All the methods detected the 61 MRSA strains having the mecA gene, showing 100% sensitivity and specificity. Despite the correlation between all the rapid methods and PCR, the ease of use and shorter turnaround time of MRSA-Screen were important factors leading to the selection of this method as the routine screening technique for MRSA.

Key words:
MRSA
MRSA-Screen
BBL Crystal
Velogene
mecA-PCR

Se han evaluado tres métodos rápidos para detectar la presencia de Staphylococcus aureus resistente a la meticilina (SARM) y se han comparado con la amplificación del gen mecA mediante reacción en cadena de la polimerasa (PCR). Se estudiaron un total de 103 cepas de S. aureus mediante MRSA-Screen, BBL Crystal, Velogene Genomics y PCR para mecA. Con todos estos métodos se detectaron 61 cepas de SARM que presentaban el gen mecA, con una sensibilidad y especificidad del 100%. A pesar de la correlación entre todos los métodos rápidos y la PCR, la facilidad de uso y el poco tiempo que lleva a la realización de MRSA-Screen fueron factores importantes para la selección de este método como técnica sistemática de detección de SARM.

Palabras clave:
SARM
MRSA-Screen
BBL Crystal
Velogene
mecA-PCR
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Copyright © 2004. Elsevier España, S.L.. All rights reserved
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