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Vol. 22. Issue 2.
Pages 62-67 (May - August 2021)
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Vol. 22. Issue 2.
Pages 62-67 (May - August 2021)
Original article
Identification of Helicobacter pylori in tumor biopsies obtained from patients with colorectal cancer: Indication for a prophylactic vaccine?
Identificación de Helicobacter pylori en biopsias tumorales obtenidas de pacientes con cáncer colorrectal: ¿indicación de una vacuna profiláctica?
N. Kamalia, A. Talebi Bezmin Abadia,?
Corresponding author
Amin.talebi@modares.ac.ir

Corresponding author.
, F. Rahimib, M. Forootanc
a Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
b Research School of Biology, The Australian National University, Canberra, Australia
c Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Tehran, Iran
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Table 1. Distribution of levels of active inflammation in the 39 biopsy samples.
Abstract

The association between H. pylori infection and colorectal cancer (CRC) has been controversial, and despite the undisputed roles of H. pylori in the etiology of gastric cancer, its involvement as a causative agent of CRC is unknown. We aimed to investigate the potential associations between H. pylori infection and CRC in biopsy samples taken from tumors of Iranian patients. Patients (n=49; aged 38–79 years, mean age=49) with diagnosed CRC were enrolled. Biopsies from control (n=10) and suspected CRC patients were collected from March 2017 to 1 August 2019 at the Mehrad Hospital, Tehran, Iran. The diagnosis of CRC was confirmed by pathology and colonoscopic examinations. An expert gastroenterologist performed the colonoscopic surgery and sample collection. DNA was extracted from the samples, and H. pylori infection was detected and confirmed by PCR using the glmM sequence as the species-specific gene. SPSS version 18 was used to determine the significant associations by using the two-tailed Fisher's exact test. Eighteen (46.1%) and 6 (60%) patients were female in the CRC and control groups, respectively. No significant associations were observed between H. pylori infection and family history of cancer, age, or gender. Thirty-one (79%) CRC samples showed severe inflammation. Out of the 31 CRC samples with severe inflammation, 18 (58%) were positive for H. pylori (P<0.05). We conclude that H. pylori may be involved in the CRC pathogenesis or progression. Our findings suggest that a prophylactic vaccine against H. pylori may be promising in reducing the CRC incidence.

Keywords:
Colorectal cancer
Helicobacter pylori
PCR
Prevention
Screening program
Tumor
Vaccine
Resumen

La asociación entre la infección por H. pylori y el cáncer colorrectal (CCR) ha sido controversial y, a pesar del papel indiscutible de H. pylori en la etiología del cáncer gástrico, se desconoce su participación como agente causante del CCR. Nuestro objetivo consistió en investigar las posibles asociaciones entre la infección por H. pylori y el CCR en muestras de biopsia tomadas de tumores de pacientes iraníes. Pacientes (n = 49) de 38 a 79 años de edad (edad media = 49) con diagnóstico de CCR fueron admitidos. Las biopsias de los pacientes de control (n = 10) y sospechosos de CCR se recolectaron desde marzo de 2017 hasta el 1 de agosto de 2019 en el Hospital Mehrad, Teherán, Irán. El diagnóstico de CCR se confirmó mediante exámenes anatomopatológicos y colonoscópicos. Un gastroenterólogo experto realizó la cirugía colonoscópica y la toma de muestras. Se extrajo ADN de las muestras y se detectó y confirmó la infección por H. pylori mediante PCR utilizando la secuencia glmM como gen específico de la especie. Se utilizó la versión 18 de SPSS para determinar las asociaciones significativas mediante la prueba exacta de Fisher de dos colas. Dieciocho (46,1%) y 6 (60%) pacientes en el grupo de CCR y de control, respectivamente, fueron mujeres. No se observaron asociaciones significativas entre la infección por H. pylori y los antecedentes familiares de cáncer, la edad o el sexo. Treinta y una (79%) muestras de CRC mostraron inflamación severa. De las 31 muestras de CCR con inflamación severa, 18 (58%) fueron positivas para H. pylori (P < 0,05). Concluimos que H. pylori puede estar involucrado en la patogénesis o progresión del CCR. Nuestros hallazgos sugieren que una vacuna profiláctica contra H. pylori puede ser prometedora para reducir la incidencia de CCR.

Palabras clave:
Cáncer colorrectal
Helicobacter pylori
PCR
Prevención
Programa de cribado
Tumor
Vacuna

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