P-20 OXIDATIVE STRESS MARKERS IN ALCOHOLIC LIVER DISEASE

Introduction and Objectives

Several mechanisms participate in the physiopathology of Alcoholic Liver Disease (ALD), such as deregulation in the immune system and oxidative stress. Aim: To analyze markers of lipoperoxidation and oxidative proteins in patients with ALD: Alcohol hepatitis (AH) and liver cirrhosis (CiR).

Materials and methods

This transversal study included 220 individuals divided into three groups: the control group (n=100), individuals with alcohol consumption 10 g/day and AUDIT score 7, the group of AH patients (n=45) and CiR patients (n=75). We measured the serum levels of MDA (thiobarbituric acid method) and carbonylated proteins (DNPH reaction). The statistical analysis was performed by the SPSS v25 software. Data expressed as mean values ± SEM, p-value <0.05 was considered statistically significant.

Results

The control group (CT), with 30.47±0.52 years old, alcohol consumption of 2.32±0.21 gOH/day and AUDIT 2.24±0.10. The ALD patients, had 41.68±6.3 years old, consumption of 354.25±139.54 gOH/day and AUDIT 30±5.45. The AST, ALT, GGT, total and indirect bilirubin serum were higher in AH and CiR compared to CT (p<0.001), ratio of AST/ALT 2. The albumin levels were lower (p<0.001) in AH vs. CT. The carbonylated proteins serum concentrations were higher in patients with AH compared to CT and CiR (p<0.001). Differences in MDA serum levels were found between CiR versus HA and CT groups (p< 0.005).

Conclusions

Our results suggest that carbonylated proteins and MDA are markers of oxidative damage in the alcohol hepatitis and liver cirrhosis Mexican patients. This damage may increase the risk of malnutrition, susceptibility to infections and sepsis, deficient coagulation factors production, gastrointestinal bleeding, among other complications that increase mortality. According these results is necessary to counteract oxidative damage for improving and complementing the actual treatment of alcoholic liver disease.