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Inicio Brazilian Journal of Microbiology Complete genome sequence of the aerobically denitrifying thermophilic bacterium ...
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Vol. 48. Núm. 4.
Páginas 615-616 (octubre - diciembre 2017)
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Vol. 48. Núm. 4.
Páginas 615-616 (octubre - diciembre 2017)
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Complete genome sequence of the aerobically denitrifying thermophilic bacterium Chelatococcus daeguensis TAD1
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Yunlong Yanga, Ershu Lina, Shaobin Huangb,c,
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hshbyyl@126.com

Corresponding author.
a College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, China
b The Key Laboratory of Environmental Protection and Eco-Remediation of Guangdong Regular Higher Education Institutions, Guangzhou, China
c School of Environment and Energy, South China University of Technology, Guangzhou, China
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Table 1. Features of Chelatococcus daeguensis TAD1 genome.
Abstract

Chelatococcus daeguensis TAD1 is a themophilic bacterium isolated from a biotrickling filter used to treat NOx in Ruiming Power Plant, located in Guangzhou, China, which shows an excellent aerobic denitrification activity at high temperature. The complete genome sequence of this strain was reported in the present study. Genes related to the aerobic denitrification were identified through whole genome analysis. This work will facilitate the mechanism of aerobic denitrification and provide evidence for its potential application in the nitrogen removal.

Keywords:
Chelatococcus daeguensis TAD1
Complete genome sequencing
Aerobic denitrification
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Denitrification conducted by many organisms plays a critical role in the nitrogen cycle, which is conventionally considered to be occurred anaerobically. However, some microorganisms were found to have the capacity of aerobic denitrification, such as Klebsiella pneumoniae CF-S9,1Vibrio diabolicus SF16,2Anoxybacillus contaminans HA,3Pseudomonas tolaasii Y-11,4Cupriavidus sp. S15 and so on. Due to much higher growth rate than autotrophs and capability of utilizing various substrates, these bacteria have attracted more and more attention. Nevertheless, the aerobic denitrification mechanism with respect to functional genes has not been clarified. In addition, the research on the aerobic denitrification mechanism at high temperature was seldom reported. Chelatococcus daeguensis TAD1 that can use a variety of carbon sources to denitrify aerobically at high temperature was isolated from a biotrickling filter used to treat NOx in Ruiming Power Plant, located in Guangzhou, China.6 In order to gain insights about genetic elements involved in aerobic denitrification properties and to further identify the nitrogen removal activity of this strain, we performed genome sequencing and hereby present the complete genome sequence of C. daeguensis TAD1.

Genomic DNA from TAD1 was extracted with standard genomic DNA isolation methods. Sequencing was performed using the PacBio RS II (Pacific Biosciences, USA) by constructing a 20kb insert library at NOVOGENE (Beijing, China), and 366,577,965bp were generated from 57,631 subreads. The N50 subread sequence length was 8695bp. For sequence assemblies, SMRT Analysis Software (Pacific Biosciences, USA) was used. One complete circular chromosome and one complete circular plasmid were generated. Genome annotation was performed using GeneMarkS software (http://topaz.gatech.edu/).

The complete genome sequence of TAD1 consists of a circular 3.56Mb chromosome and a circular 0.5Mb plasmid, with GC contents of 67.85% and 68.12%, respectively (Table 1). The count of predicted protein-coding sequences is 3889, and the genome comprises 48 tRNA genes and 6 tRNA operons (Table 1). We identified four genes (napA, nirK, norB and nosZ) involved in the aerobic denitrification, encoding nitrate reductase, nitrite reductase, nitric oxide reductase and nitrous oxide reductase, respectively. As a facultative thermophile, the strain TAD1 grows optimally at 45–50, and possesses a good performance of nitrogen removal.7,8 It is well known that emissions from some industries, especially in Power Plant where the temperature of flue gas (NOx) is high and a certain concentration of oxygen exists in NOx, are harmful to those microorganisms at room temperature, indicating that thermophilic bacteria are necessary for the efficient nitrogen removal under aerobic conditions. Therefore, the genes relating to the aerobic denitrification activity of this strain were enough to evaluate its application in those industries with high temperature of emissions.

Table 1.

Features of Chelatococcus daeguensis TAD1 genome.

Attributes  Values 
Genome size (bp)  4,095,174 
GC content (%)  68.34 
Plasmid 
rRNAs 
tRNAs  48 
ncRNA  55 
CDSs  3889 

The complete genome sequence of C. daeguensis TAD1 will enrich the sources of thermostable denitrifying enzymes, facilitate the aerobic denitrification mechanism and eventually exhibit basic information for wider exploitation of this strain in nitrogen removal.

The complete genome sequence of C. daeguensis TAD1 was deposited at GenBank under accession number CP018095CP018096. This strain has been deposited in China General Microbiological Culture Collection Center (CGMCC no. 5226).

Conflict of interest

We declare that we have no conflict of interest.

Acknowledgements

This work was supported by the National Natural Science Foundation of China (no. 21407024) and the Natural Science Foundation of Fujian Province (no. 2015J01594).

References
[1]
S.K. Padhi, S. Tripathy, R. Sen, A.S. Mahapatra, S. Mohanty, N.K. Maiti.
Characterisation of heterotrophic nitrifying and aerobic denitrifying Klebsiella pneumoniae CF-S9 strain for bioremediation of wastewater.
Int Biodeter Biodegr, 78 (2013), pp. 67-73
[2]
J.M. Duan, H.D. Fang, B. Su, J.F. Chen, J.M. Lin.
Characterization of a halophilic heterotrophic nitrification–aerobic denitrification bacterium and its application on treatment of saline wastewater.
Bioresourc Technol, 179 (2015), pp. 421-428
[3]
J. Chen, J. Zheng, Y. Li, H.H. Hao, J.M. Chen.
Characteristics of a novel thermophilic heterotrophic bacterium, Anoxybacillus contaminans HA, for nitrification-aerobic denitrification.
Appl Microbiol Biotechnol, 99 (2015), pp. 10695-10702
[4]
T.X. He, Z.L. Li, Q. Sun, Y. Xu, Q. Ye.
Heterotrophic nitrification and aerobic denitrification by Pseudomonas tolaasii Y-11 without nitrite accumulation during nitrogen conversion.
Bioresourc Technol, 200 (2016), pp. 493-499
[5]
Z. Sun, Y. Lv, Y. Liu, R. Ren.
Removal of nitrogen by heterotrophic nitrification-aerobic denitrification of a novel metal resistant bacterium Cupriavidus sp. S1.
Bioresourc Technol, 220 (2016), pp. 142-150
[6]
W. Liang, S. Huang, J. Liu, R. Zhang, F. Yan.
Removal of nitric oxide in a biotrickling filter under thermophilic condition using Chelatococcus daeguensis.
J Air Waste Manage Assoc, 62 (2012), pp. 509-516
[7]
Y. Yang, S. Huang, W. Liang, Y. Zhang, H. Huang, F. Xu.
Microbial removal of NOx at high temperature by a novel aerobic strain Chelatococcus daeguensis TAD1 in a biotrickling filter.
J Hazard Mater, 203 (2012), pp. 326-332
[8]
W. Liang, S. Huang, Y. Yang, R. Jiang.
Experimental and modeling study on nitric oxide removal in a biotrickling filter using Chelatococcus daeguensis under thermophilic condition.
Bioresourc Technol, 125 (2012), pp. 82-87
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